WO2008099451A1 - 網羅的アミノ酸置換による高機能蛋白の設計方法 - Google Patents

網羅的アミノ酸置換による高機能蛋白の設計方法 Download PDF

Info

Publication number
WO2008099451A1
WO2008099451A1 PCT/JP2007/052342 JP2007052342W WO2008099451A1 WO 2008099451 A1 WO2008099451 A1 WO 2008099451A1 JP 2007052342 W JP2007052342 W JP 2007052342W WO 2008099451 A1 WO2008099451 A1 WO 2008099451A1
Authority
WO
WIPO (PCT)
Prior art keywords
prion
prion protein
amino acid
type
acid substitution
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/JP2007/052342
Other languages
English (en)
French (fr)
Inventor
Tetsuyuki Kitamoto
Atsushi Kobayashi
Masahiro Asano
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Tohoku University
Central Institute for Experimental Medicine and Life Science
Original Assignee
Tohoku University
Central Institute for Experimental Animals
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Tohoku University, Central Institute for Experimental Animals filed Critical Tohoku University
Priority to PCT/JP2007/052342 priority Critical patent/WO2008099451A1/ja
Publication of WO2008099451A1 publication Critical patent/WO2008099451A1/ja
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2790/00—Viroids or subviral agents
    • C12N2790/00011—Details
    • C12N2790/10011—Prions
    • C12N2790/10022—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Virology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

プリオン蛋白に点変異を導入して、異常型になりやすいプリオン蛋白の分子設計を行うことを目的とする。プリオン蛋白の正常型から異常型への変換能を促進するアミノ酸置換を有するプリオン蛋白を設計する方法であって、プリオン蛋白のアミノ酸を網羅的に置換した複数の変異プリオン蛋白から構成される変異プリオン蛋白を得て、該変異プリオン蛋白の正常型から異常型への変換能を測定し、正常型から異常型への変換能が野生型プリオン蛋白に比較して促進された変異プリオン蛋白を選択することを含む、プリオン蛋白の正常型から異常型への変換能を促進するアミノ酸置換を有するプリオン蛋白を設計する方法。
PCT/JP2007/052342 2007-02-09 2007-02-09 網羅的アミノ酸置換による高機能蛋白の設計方法 Ceased WO2008099451A1 (ja)

Priority Applications (1)

Application Number Priority Date Filing Date Title
PCT/JP2007/052342 WO2008099451A1 (ja) 2007-02-09 2007-02-09 網羅的アミノ酸置換による高機能蛋白の設計方法

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/JP2007/052342 WO2008099451A1 (ja) 2007-02-09 2007-02-09 網羅的アミノ酸置換による高機能蛋白の設計方法

Publications (1)

Publication Number Publication Date
WO2008099451A1 true WO2008099451A1 (ja) 2008-08-21

Family

ID=39689711

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2007/052342 Ceased WO2008099451A1 (ja) 2007-02-09 2007-02-09 網羅的アミノ酸置換による高機能蛋白の設計方法

Country Status (1)

Country Link
WO (1) WO2008099451A1 (ja)

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998036059A2 (de) * 1997-02-14 1998-08-20 Deutsches Krebsforschungszentrum Stiftung des öffentlichen Rechts Hemmung der prion-vermehrung durch dominant-negative prionprotein-mutanten
JPH1156365A (ja) * 1997-08-21 1999-03-02 Seibutsu Bunshi Kogaku Kenkyusho:Kk 変異体ケクスタチン
WO2001027152A1 (en) * 1999-10-15 2001-04-19 Isis Innovation Limited Method of altering polypeptide aggregation
WO2001042453A1 (fr) * 1999-12-06 2001-06-14 Biomolecular Engineering Research Institute Coordonnees structurales et decalage chimique rmn de proteine; utilisation
WO2004007546A1 (en) * 2002-07-11 2004-01-22 Eidgenoessische Technische Hochschule Zuerich Mutant proteins and use thereof for the manufacture of medicaments and the treatment of humans or animals suffering from conformational diseases

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998036059A2 (de) * 1997-02-14 1998-08-20 Deutsches Krebsforschungszentrum Stiftung des öffentlichen Rechts Hemmung der prion-vermehrung durch dominant-negative prionprotein-mutanten
JPH1156365A (ja) * 1997-08-21 1999-03-02 Seibutsu Bunshi Kogaku Kenkyusho:Kk 変異体ケクスタチン
WO2001027152A1 (en) * 1999-10-15 2001-04-19 Isis Innovation Limited Method of altering polypeptide aggregation
WO2001042453A1 (fr) * 1999-12-06 2001-06-14 Biomolecular Engineering Research Institute Coordonnees structurales et decalage chimique rmn de proteine; utilisation
WO2004007546A1 (en) * 2002-07-11 2004-01-22 Eidgenoessische Technische Hochschule Zuerich Mutant proteins and use thereof for the manufacture of medicaments and the treatment of humans or animals suffering from conformational diseases

Similar Documents

Publication Publication Date Title
TW200726845A (en) Biomarker molecular of renal illness and detecting method for the same
GB2509284A (en) Endoglycosidase from streptococcus pyogenes and methods using it
CA2783820C (en) Protease variants
WO2010135836A8 (en) Novel beta-glucosidase enzymes
BRPI0715243A2 (pt) "método de aumento da produção de etanol e linhagem de z. mobilis"
ZA200809973B (en) Glycolic acid production by fermentation from renewable resources
WO2011100541A3 (en) Compositions and methods for the detection of small rnas
WO2006128685A3 (en) Process for making a low molecular weight gelatine hydrolysate and gelatine hydrolysate compositions
WO2008153925A3 (en) Methods for improving multiple protein properties
WO2010088411A3 (en) Conformationally dynamic peptides
WO2009114702A3 (en) Recombinant production of authentic human proteins using human cell expression systems
WO2011026906A3 (en) Novel microorganism and its use in lignocellulose detoxification
WO2011044458A8 (en) Compositions and methods for diagnosing genome related diseases and disorders
WO2007109441A3 (en) Polypeptides having endoglucanase activity and polynucleotides encoding same
WO2007115201A8 (en) Polypeptides having endoglucanase activity and polynucleotides encoding same
WO2007106738A3 (en) Acrylated hyaluronic acid
WO2008126784A1 (ja) グルタチオンおよびγ-グルタミルシステインの製造法
WO2010115745A3 (en) Biomarkers, methods and kits for the diagnosis of rheumatoid arthritis
IL202327A (en) An isolated cyclic protein, an isolated nucleic acid encoded for this protein, a host cell containing this nucleic acid sequence and a process for making this cyclic protein
WO2010015899A3 (en) Novel method to generate meganucleases with altered characteristics
EP1983048A4 (en) NEW PROTEIN EXPRESSION SYSTEM
WO2007144508A3 (fr) Arn de transfert chimerique et son utilisation pour la production d'arn par une cellule
WO2009051109A1 (ja) 異種タンパク質を高生産する細胞の作製方法
WO2008091948A3 (en) Galectin-3-binding, protein as a biomarker of cardiovascular disease
WO2009147103A3 (en) Method for producing a wheat protein hydrolysate

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 07713998

Country of ref document: EP

Kind code of ref document: A1

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 07713998

Country of ref document: EP

Kind code of ref document: A1

NENP Non-entry into the national phase

Ref country code: JP