WO2008097828A8 - Method for isolating mesenchymal stromal cells - Google Patents

Method for isolating mesenchymal stromal cells Download PDF

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Publication number
WO2008097828A8
WO2008097828A8 PCT/US2008/052759 US2008052759W WO2008097828A8 WO 2008097828 A8 WO2008097828 A8 WO 2008097828A8 US 2008052759 W US2008052759 W US 2008052759W WO 2008097828 A8 WO2008097828 A8 WO 2008097828A8
Authority
WO
WIPO (PCT)
Prior art keywords
stromal cells
mesenchymal stromal
isolating mesenchymal
isolating
mscs
Prior art date
Application number
PCT/US2008/052759
Other languages
French (fr)
Other versions
WO2008097828A3 (en
WO2008097828A2 (en
Inventor
Edwin M. Horwitz
Original Assignee
St. Jude Children's Research Hospital
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by St. Jude Children's Research Hospital filed Critical St. Jude Children's Research Hospital
Publication of WO2008097828A2 publication Critical patent/WO2008097828A2/en
Publication of WO2008097828A3 publication Critical patent/WO2008097828A3/en
Publication of WO2008097828A8 publication Critical patent/WO2008097828A8/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0667—Adipose-derived stem cells [ADSC]; Adipose stromal stem cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/35—Fat tissue; Adipocytes; Stromal cells; Connective tissues
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0663—Bone marrow mesenchymal stem cells (BM-MSC)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0665—Blood-borne mesenchymal stem cells, e.g. from umbilical cord blood

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Cell Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Rheumatology (AREA)
  • Hematology (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Virology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The present invention relates to an isolated population of non-cancerous cells which have been enriched for ganglioside GD2 mesenchymal stromal cells (MSCs). Methods for isolating GD2+ MSCs and using the same in therapeutic applications are also provided.
PCT/US2008/052759 2007-02-02 2008-02-01 Method for isolating mesenchymal stromal cells WO2008097828A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US88784407P 2007-02-02 2007-02-02
US60/887,844 2007-02-02

Publications (3)

Publication Number Publication Date
WO2008097828A2 WO2008097828A2 (en) 2008-08-14
WO2008097828A3 WO2008097828A3 (en) 2008-11-13
WO2008097828A8 true WO2008097828A8 (en) 2009-09-03

Family

ID=39682340

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2008/052759 WO2008097828A2 (en) 2007-02-02 2008-02-01 Method for isolating mesenchymal stromal cells

Country Status (1)

Country Link
WO (1) WO2008097828A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EA029577B1 (en) 2011-07-06 2018-04-30 Селл Терапи Лимитед Progenitor cells of mesodermal lineage
EP3807398A4 (en) * 2018-06-14 2022-03-09 Abraham J and Phyllis Katz Cord Blood Foundation Isolation of mesenchymal stromal cells from umbilical cord blood

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2444706C (en) * 2001-04-20 2015-06-30 Children's Hospital Of Orange County Isolation of neural stem cells using gangliosides and other surface markers

Also Published As

Publication number Publication date
WO2008097828A3 (en) 2008-11-13
WO2008097828A2 (en) 2008-08-14

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