WO2008096781A1 - Betacellulin mutein - Google Patents

Betacellulin mutein Download PDF

Info

Publication number
WO2008096781A1
WO2008096781A1 PCT/JP2008/051933 JP2008051933W WO2008096781A1 WO 2008096781 A1 WO2008096781 A1 WO 2008096781A1 JP 2008051933 W JP2008051933 W JP 2008051933W WO 2008096781 A1 WO2008096781 A1 WO 2008096781A1
Authority
WO
WIPO (PCT)
Prior art keywords
amino acid
acid sequence
betacellulin mutein
lying
activity
Prior art date
Application number
PCT/JP2008/051933
Other languages
French (fr)
Japanese (ja)
Inventor
Masaharu Seno
Itaru Kojima
Original Assignee
National University Corporation Okayama University
National University Corporation Gunma University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by National University Corporation Okayama University, National University Corporation Gunma University filed Critical National University Corporation Okayama University
Priority to JP2008557137A priority Critical patent/JPWO2008096781A1/en
Publication of WO2008096781A1 publication Critical patent/WO2008096781A1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
    • A61P3/10—Drugs for disorders of the metabolism for glucose homeostasis for hyperglycaemia, e.g. antidiabetics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475—Growth factors; Growth regulators
    • C07K14/485—Epidermal growth factor [EGF] (urogastrone)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0676—Pancreatic cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/11—Epidermal growth factor [EGF]

Abstract

Disclosed is a betacellulin mutein which is a stable protein, has low antigenicity, retains an activity of causing the differentiation of an undifferentiated pancreatic stem cell into an islet beta-cell, and has a reduced cell proliferation activity. Specifically disclosed is a betacellulin mutein having an amino acid sequence corresponding to an amino acid sequence lying between position-38 and position-62 in the amino acid sequence depicted in SEQ ID NO:1 (provided that Glu at position-57 is substituted by a basic or aromatic amino acid residue, and the amino acid sequence corresponding to an amino acid sequence lying between position-38 and position-50 may have the substitution, insertion and/or deletion of one or more amino acid residues).
PCT/JP2008/051933 2007-02-07 2008-02-06 Betacellulin mutein WO2008096781A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2008557137A JPWO2008096781A1 (en) 2007-02-07 2008-02-06 Betacellulin mutein

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007028186 2007-02-07
JP2007-028186 2007-02-07

Publications (1)

Publication Number Publication Date
WO2008096781A1 true WO2008096781A1 (en) 2008-08-14

Family

ID=39681687

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2008/051933 WO2008096781A1 (en) 2007-02-07 2008-02-06 Betacellulin mutein

Country Status (2)

Country Link
JP (1) JPWO2008096781A1 (en)
WO (1) WO2008096781A1 (en)

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH09188630A (en) * 1995-11-09 1997-07-22 Takeda Chem Ind Ltd Medicine for improving hepatic function
JP2000198744A (en) * 1998-10-30 2000-07-18 Takeda Chem Ind Ltd Beta-cellulin protein-containing formulation
JP2000312591A (en) * 1998-12-09 2000-11-14 Takeda Chem Ind Ltd Betacellulin modifier

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH09188630A (en) * 1995-11-09 1997-07-22 Takeda Chem Ind Ltd Medicine for improving hepatic function
JP2000198744A (en) * 1998-10-30 2000-07-18 Takeda Chem Ind Ltd Beta-cellulin protein-containing formulation
JP2000312591A (en) * 1998-12-09 2000-11-14 Takeda Chem Ind Ltd Betacellulin modifier

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
OGATA T. ET AL.: "Betacellulin-delta4, a novel differentiation factor for pancreatic beta-cells, ameliorates glucose intolerance in streptozotocin-treated rats", ENDOCRINOLOGY, vol. 146, no. 11, 2005, pages 4673 - 4681, XP002540696, DOI: doi:10.1210/en.2005-0456 *

Also Published As

Publication number Publication date
JPWO2008096781A1 (en) 2010-05-20

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