WO2008093828A1 - Method and member for analysis of molecule on the surface of living cell - Google Patents

Method and member for analysis of molecule on the surface of living cell Download PDF

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Publication number
WO2008093828A1
WO2008093828A1 PCT/JP2008/051638 JP2008051638W WO2008093828A1 WO 2008093828 A1 WO2008093828 A1 WO 2008093828A1 JP 2008051638 W JP2008051638 W JP 2008051638W WO 2008093828 A1 WO2008093828 A1 WO 2008093828A1
Authority
WO
WIPO (PCT)
Prior art keywords
cells
molecule
substrate
disclosed
analysis
Prior art date
Application number
PCT/JP2008/051638
Other languages
French (fr)
Japanese (ja)
Inventor
Jun Hirabayashi
Hiroaki Tateno
Noboru Uchiyama
Original Assignee
National Institute Of Advanced Industrial Science And Technology
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by National Institute Of Advanced Industrial Science And Technology filed Critical National Institute Of Advanced Industrial Science And Technology
Publication of WO2008093828A1 publication Critical patent/WO2008093828A1/en

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54386—Analytical elements
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6452—Individual samples arranged in a regular 2D-array, e.g. multiwell plates
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/648—Specially adapted constructive features of fluorimeters using evanescent coupling or surface plasmon coupling for the excitation of fluorescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966—Animal cells
    • G01N33/56972—White blood cells

Abstract

Disclosed is a method for observing the information on the structure of a molecule located on the surface of a cell of interest while the cell lives. The method is achieved by contacting an array substrate having, immobilized thereon, multiple molecules capable of binding to the cells with a solution containing the cells in a fluorescently-labeled and living state, removing redundant cells which are non-specifically adsorbed on the surface of the substrate, and observing the intensity of fluorescence emitted from the cells. Also disclosed is a method for precipitating and removing the redundant cells. Further disclosed are an array substrate and an apparatus for immersing the substrate both for use in the methods.
PCT/JP2008/051638 2007-02-02 2008-02-01 Method and member for analysis of molecule on the surface of living cell WO2008093828A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007023754A JP4982793B2 (en) 2007-02-02 2007-02-02 Live cell surface molecule analysis method and member
JP2007-023754 2007-02-02

Publications (1)

Publication Number Publication Date
WO2008093828A1 true WO2008093828A1 (en) 2008-08-07

Family

ID=39674131

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2008/051638 WO2008093828A1 (en) 2007-02-02 2008-02-01 Method and member for analysis of molecule on the surface of living cell

Country Status (2)

Country Link
JP (1) JP4982793B2 (en)
WO (1) WO2008093828A1 (en)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010131641A1 (en) * 2009-05-11 2010-11-18 独立行政法人国立成育医療研究センター Method for determination of condition of cell
WO2013065302A1 (en) * 2011-11-01 2013-05-10 独立行政法人産業技術総合研究所 Undifferentiated cell detection method and complex carbohydrate detection method
WO2013186946A1 (en) * 2012-06-11 2013-12-19 国立大学法人北海道大学 Method for selecting pluripotent stem cell
WO2021011712A1 (en) * 2019-07-16 2021-01-21 Case Western Reserve University Methods for microscopy with ultraviolet surface excitation(muse) imaging

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2331934B1 (en) * 2008-09-16 2020-01-01 Pacific Biosciences of California, Inc. Analytic device including a zero mode waveguide substrate
JP5152917B2 (en) * 2008-11-04 2013-02-27 富士フイルム株式会社 Detection method, detection sample cell and detection kit
JP5095663B2 (en) * 2009-03-31 2012-12-12 独立行政法人科学技術振興機構 Fluorescence microscope, fluorescence observation method
JP5773415B2 (en) * 2011-04-19 2015-09-02 学校法人 東洋大学 Method for detecting glycated protein and biosensor chip for detecting glycated protein
JP7445966B2 (en) * 2020-03-24 2024-03-08 国立研究開発法人産業技術総合研究所 How to analyze sugar chains

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2007003357A (en) * 2005-06-23 2007-01-11 National Institute Of Advanced Industrial & Technology Analyzer for sugar chain or glycoconjugate

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2007003357A (en) * 2005-06-23 2007-01-11 National Institute Of Advanced Industrial & Technology Analyzer for sugar chain or glycoconjugate

Non-Patent Citations (10)

* Cited by examiner, † Cited by third party
Title
DISNEY M.D. ET AL.: "The use of carbohydrate microarrays to study carbohydrate-cell interactions and to detect pathogens", CHEMISTRY & BIOLOGY, vol. 11, 2004, pages 1701 - 1707, XP004689694, DOI: doi:10.1016/j.chembiol.2004.10.011 *
EBE Y. ET AL.: "Application of lectin microarray to crude samples: Differential glycan profiling of Lec mutants", J. BIOCHEM., vol. 139, no. 3, 2006, pages 323 - 327 *
EBE Y. ET AL.: "Lectin Microarray no Kaihatsu; Evanescent-field Reikigata Keiko Scanner de High Through-put na Tosa Kaiseki", BIONICS, vol. 3, no. 5, 2006, pages 58 - 59 *
HSU K.-L. ET AL.: "A lectin microarray approach for the rapid analysis of bacterial glycans", NATURE PROTOCOLS, vol. 1, no. 2, 2006, pages 543 - 549 *
HSU K.-L. ET AL.: "Analyzing the dynamic bacterial glycome with a lectin microarray approach", NATURE CHEMICAL SOCIETY, vol. 2, no. 3, 2006, pages 153 - 157 *
KUNO A. ET AL.: "Evanescent-field fluorescence-assisted lectin microarra: a new strategy for glycan profiling", NATURE METHODS, vol. 2, no. 11, 2005, pages 851 - 856, XP002449064 *
KUNO S. ET AL.: "Evanescentfield Reikiho ni yoru Lectin Microarray System", NEW SCIENCE CO., LTD., MEDICAL SCIENCE DIGEST, vol. 32, no. 11, 2006, pages 473 - 474 *
PILOBELLO K.T. ET AL.: "A ratiometric lectin microarray approach to analysis of the dynamic mammalian glycome", PNAS, vol. 104, no. 28, 10 July 2007 (2007-07-10), pages 11534 - 11539 *
TATENO H. ET AL.: "A novel strategy for mammalian cell surface glycome profiling using lectin microarray", GLYCOBIOLOGY, vol. 17, no. 10, October 2007 (2007-10-01), pages 1138 - 1146, XP055185875, DOI: doi:10.1093/glyco/cwm084 *
UCHIYAMA N. ET AL.: "Development of a lectin microarray based on an evanescent-field fluorescence principle", METHODS IN ENZYMOLOGY, vol. 415, 2006, pages 341 - 351 *

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010131641A1 (en) * 2009-05-11 2010-11-18 独立行政法人国立成育医療研究センター Method for determination of condition of cell
JPWO2010131641A1 (en) * 2009-05-11 2012-11-01 独立行政法人国立成育医療研究センター How to determine cell status
WO2013065302A1 (en) * 2011-11-01 2013-05-10 独立行政法人産業技術総合研究所 Undifferentiated cell detection method and complex carbohydrate detection method
US9500650B2 (en) 2011-11-01 2016-11-22 National Institute Of Advanced Industrial Science And Technology Undifferentiated cell detection method and complex carbohydrate detection method
WO2013186946A1 (en) * 2012-06-11 2013-12-19 国立大学法人北海道大学 Method for selecting pluripotent stem cell
JPWO2013186946A1 (en) * 2012-06-11 2016-02-01 国立大学法人北海道大学 Method for sorting pluripotent stem cells
WO2021011712A1 (en) * 2019-07-16 2021-01-21 Case Western Reserve University Methods for microscopy with ultraviolet surface excitation(muse) imaging

Also Published As

Publication number Publication date
JP4982793B2 (en) 2012-07-25
JP2008187932A (en) 2008-08-21

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