WO2008084566A1 - Model mouse, method of constructing the same and use thereof - Google Patents

Model mouse, method of constructing the same and use thereof Download PDF

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Publication number
WO2008084566A1
WO2008084566A1 PCT/JP2007/063756 JP2007063756W WO2008084566A1 WO 2008084566 A1 WO2008084566 A1 WO 2008084566A1 JP 2007063756 W JP2007063756 W JP 2007063756W WO 2008084566 A1 WO2008084566 A1 WO 2008084566A1
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WO
WIPO (PCT)
Prior art keywords
gsdmd
mouse
cells
gene
model mouse
Prior art date
Application number
PCT/JP2007/063756
Other languages
French (fr)
Japanese (ja)
Inventor
Toshihiko Shiroishi
Masaru Tamura
Tomoaki Fujii
Original Assignee
Research Organization Of Information And Systems
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Research Organization Of Information And Systems filed Critical Research Organization Of Information And Systems
Priority to JP2008552997A priority Critical patent/JPWO2008084566A1/en
Publication of WO2008084566A1 publication Critical patent/WO2008084566A1/en

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • A01K67/0276—Knock-out vertebrates
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4702—Regulators; Modulating activity
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5082—Supracellular entities, e.g. tissue, organisms
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/07—Animals genetically altered by homologous recombination
    • A01K2217/075—Animals genetically altered by homologous recombination inducing loss of function, i.e. knock out
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/105—Murine
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/03—Animal model, e.g. for test or diseases

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Cell Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Toxicology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Organic Chemistry (AREA)
  • Environmental Sciences (AREA)
  • Zoology (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Veterinary Medicine (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

It is intended to provide a model mouse which is to be used for clarifying the functions of Gsdmd on intestinal epithelial cells and Gsdmd gene encoding the same. A mouse having a homozygote in which Gsdmd gene encoding Gsdmd has been knock-out on chromosome and not expressing Gsdmd is referred to as the model mouse. First, a targeting vector having a fragment which contains the exon 2 of Gsdmd gene in mouse genomic DNA is transferred into mouse embryonic stem cells and chimeric embryonic cells are constructed from the embryonic stem cells having the Gsdmd gene knock-out by homologous recombination. Next, these chimeric cells are transplanted into a host mouse. The Gsdmd gene-knock out chimeric mice thus bred are mated and heterozygous mice are selected. By mating these heterozygous mice with each other, a model mouse of the Gsdmd gene-knockout homozygote can be obtained.
PCT/JP2007/063756 2007-01-11 2007-07-10 Model mouse, method of constructing the same and use thereof WO2008084566A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2008552997A JPWO2008084566A1 (en) 2007-01-11 2007-07-10 Model mouse, manufacturing method thereof and use thereof

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007003398 2007-01-11
JP2007-003398 2007-01-11

Publications (1)

Publication Number Publication Date
WO2008084566A1 true WO2008084566A1 (en) 2008-07-17

Family

ID=39608467

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2007/063756 WO2008084566A1 (en) 2007-01-11 2007-07-10 Model mouse, method of constructing the same and use thereof

Country Status (2)

Country Link
JP (1) JPWO2008084566A1 (en)
WO (1) WO2008084566A1 (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2018214823A1 (en) * 2017-05-25 2018-11-29 朱献军 METHOD FOR CONSTRUCTING MOUSE MODEL WITH CONDITIONAL KNOCKOUT OF TMEM30A GENE FROM PANCREATIC β CELL AND USE
CN114657214A (en) * 2022-02-25 2022-06-24 陕西中医药大学 Construction method of spontaneous kidney-yin deficiency Ptgds gene knockout rat model
CN115725687A (en) * 2022-10-08 2023-03-03 浙江大学 Screening method of pig-derived antibacterial short peptide

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004076666A1 (en) * 2003-02-28 2004-09-10 Toshihiko Shiroishi Gasdermin family
WO2006019153A1 (en) * 2004-08-20 2006-02-23 Inter University Research Institution Corporation, Research Organization Of Information And Systems NONHUMAN ANIMAL WITH INHIBITED GdsmA GENE FUNCTION AND PROMOTED OR INHIBITIED CANCERATION-ASSOCIATED GENE FUNCTION

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004076666A1 (en) * 2003-02-28 2004-09-10 Toshihiko Shiroishi Gasdermin family
WO2006019153A1 (en) * 2004-08-20 2006-02-23 Inter University Research Institution Corporation, Research Organization Of Information And Systems NONHUMAN ANIMAL WITH INHIBITED GdsmA GENE FUNCTION AND PROMOTED OR INHIBITIED CANCERATION-ASSOCIATED GENE FUNCTION

Non-Patent Citations (10)

* Cited by examiner, † Cited by third party
Title
FUJII T. ET AL.: "Expression and functional analyses of GasderminD(GsdmD)", GENES & GENETIC SYSTEMS, vol. 80, no. 6, 2005, pages 462, XP003022943 *
FUJII T. ET AL.: "Gasdermin (Gsdm) is involved in maintaining of homeostasis of intestinal epithelial ceels in intestinal tract", GENES & GENETIC SYSTEMS, vol. 81, no. 6, December 2006 (2006-12-01), pages 450, XP003022935 *
FUJII T. ET AL.: "Gasdermin(Gsdm)family Idenshi no Tanri to sono Hatsugen Kaiseki", JAPAN SOCIETY OF DEVELOPMENT BIOLOGISTS TAIKAI HAPPYO YOSHISHU, vol. 36TH, 2003, pages 55, XP002996880 *
FUJII T. ET AL.: "Shokakan ni Oite, Gasdermin (Gsdm) wa Johi Saibo no Kojosei Iji ni Kanyo suru", 78TH ANNUAL MEETING OF THE GENETICS SOECIETY OF JAPAN, PROGRAMS & ABSTRACTS, 1 September 2006 (2006-09-01), pages 164, 2E08, XP003022936 *
FUJII T. ET AL.: "Shokakan no Oite, Gasdermin (Gsdm) wa Johi Saibo no Kojosei Iji ni Kanyo suru", THE GENETICS SOCIETY OF JAPAN, 26 September 2006 (2006-09-26), XP003022942, Retrieved from the Internet <URL:http://www.brc.riken.jp/iden78/program2.pdf> *
FUJII T.: "Expression profiling and functional analysis of Gasdermin (Gsdm) family genes in mouse intestinal tract", 28 August 2006 (2006-08-28), XP003022937, Retrieved from the Internet <URL:http://www.nig.ac.jp/cgi-bin/seminar.pl?action=display&target=20060828005_&lang=Japanese> *
KATOH M. ET AL.: "Identification and characterization of human DFNA5L, mouse Dfna5l, and rat Dfna5l genes in silico", INTERNATIONAL JOURNAL OF ONCOLOGY, vol. 25, no. 3, 2004, pages 765 - 770, XP003022938 *
SUN Q. ET AL.: "Progress of researches on gene function of GSDMDC family", YI CHUAN, vol. 28, no. 5, May 2006 (2006-05-01), pages 596 - 600, XP003022939 *
TAMURA M. ET AL.: "Gasdermin(Gsdm/GSDM) family no Hatsugen to Shinkateki Sokumen ni Tsuite", JAPAN SOCIETY OF DEVELOPMENT BIOLOGISTS TAIKAI HAPPYO YOSHISHU, vol. 39TH, 2006, pages 174, XP003022940 *
TAMURA M. ET AL.: "Shinki Gsdm family Isenshi no Tanri to sono Hatsugen Kaiseki", JAPAN SOCIETY OF DEVELOPMENT BIOLOGISTS TAIKAI HAPPYO YOSHISHU, vol. 38TH, 2005, pages 243, XP003022941 *

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2018214823A1 (en) * 2017-05-25 2018-11-29 朱献军 METHOD FOR CONSTRUCTING MOUSE MODEL WITH CONDITIONAL KNOCKOUT OF TMEM30A GENE FROM PANCREATIC β CELL AND USE
CN114657214A (en) * 2022-02-25 2022-06-24 陕西中医药大学 Construction method of spontaneous kidney-yin deficiency Ptgds gene knockout rat model
CN114657214B (en) * 2022-02-25 2024-04-16 陕西中医药大学 Construction method of spontaneous kidney-yin deficiency Ptgds gene knockout rat model
CN115725687A (en) * 2022-10-08 2023-03-03 浙江大学 Screening method of pig-derived antibacterial short peptide
CN115725687B (en) * 2022-10-08 2023-12-12 浙江大学 Screening method of pig-derived antibacterial short peptide

Also Published As

Publication number Publication date
JPWO2008084566A1 (en) 2010-04-30

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