WO2008075864A1 - Quality determination method of muskrat musk obtained from muskrat - Google Patents
Quality determination method of muskrat musk obtained from muskrat Download PDFInfo
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- WO2008075864A1 WO2008075864A1 PCT/KR2007/006585 KR2007006585W WO2008075864A1 WO 2008075864 A1 WO2008075864 A1 WO 2008075864A1 KR 2007006585 W KR2007006585 W KR 2007006585W WO 2008075864 A1 WO2008075864 A1 WO 2008075864A1
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- WIPO (PCT)
- Prior art keywords
- muskrat
- musk
- muskrat musk
- civetone
- normuscone
- Prior art date
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- 241000699700 Ondatra zibethicus Species 0.000 title claims abstract description 70
- 241000402754 Erythranthe moschata Species 0.000 title claims abstract description 59
- 238000000034 method Methods 0.000 title claims abstract description 16
- OSOIQJGOYGSIMF-UHFFFAOYSA-N cyclopentadecanone Chemical compound O=C1CCCCCCCCCCCCCC1 OSOIQJGOYGSIMF-UHFFFAOYSA-N 0.000 claims abstract description 42
- KBQDZEMXPBDNGH-UHFFFAOYSA-N cycloheptadecanone Chemical compound O=C1CCCCCCCCCCCCCCCC1 KBQDZEMXPBDNGH-UHFFFAOYSA-N 0.000 claims abstract description 40
- ZKVZSBSZTMPBQR-UHFFFAOYSA-N Civetone Natural products O=C1CCCCCCCC=CCCCCCCC1 ZKVZSBSZTMPBQR-UHFFFAOYSA-N 0.000 claims abstract description 23
- ZKVZSBSZTMPBQR-UPHRSURJSA-N civetone Chemical compound O=C1CCCCCCC\C=C/CCCCCCC1 ZKVZSBSZTMPBQR-UPHRSURJSA-N 0.000 claims abstract description 23
- 238000002290 gas chromatography-mass spectrometry Methods 0.000 claims abstract description 7
- 239000003960 organic solvent Substances 0.000 claims description 8
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 7
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 6
- 238000002137 ultrasound extraction Methods 0.000 claims description 5
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 claims description 4
- 239000007788 liquid Substances 0.000 claims description 4
- 239000012528 membrane Substances 0.000 claims description 4
- 238000001914 filtration Methods 0.000 claims description 2
- 238000005259 measurement Methods 0.000 claims description 2
- 238000005374 membrane filtration Methods 0.000 claims 1
- 239000011148 porous material Substances 0.000 claims 1
- 241001416180 Moschidae Species 0.000 description 7
- 238000004458 analytical method Methods 0.000 description 7
- 241001465754 Metazoa Species 0.000 description 6
- 230000014759 maintenance of location Effects 0.000 description 6
- 241000282375 Herpestidae Species 0.000 description 4
- 241000699705 Ondatra Species 0.000 description 4
- 239000002994 raw material Substances 0.000 description 4
- ALHUZKCOMYUFRB-OAHLLOKOSA-N Muscone Chemical group C[C@@H]1CCCCCCCCCCCCC(=O)C1 ALHUZKCOMYUFRB-OAHLLOKOSA-N 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- ALHUZKCOMYUFRB-UHFFFAOYSA-N muskone Natural products CC1CCCCCCCCCCCCC(=O)C1 ALHUZKCOMYUFRB-UHFFFAOYSA-N 0.000 description 3
- 230000000144 pharmacologic effect Effects 0.000 description 3
- 239000010421 standard material Substances 0.000 description 3
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 2
- 238000009395 breeding Methods 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- 239000002537 cosmetic Substances 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 239000003205 fragrance Substances 0.000 description 2
- 238000004817 gas chromatography Methods 0.000 description 2
- 238000002347 injection Methods 0.000 description 2
- 239000007924 injection Substances 0.000 description 2
- 238000007689 inspection Methods 0.000 description 2
- 244000144972 livestock Species 0.000 description 2
- 238000004949 mass spectrometry Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 239000002304 perfume Substances 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 241000251468 Actinopterygii Species 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 241000282326 Felis catus Species 0.000 description 1
- 241000699729 Muridae Species 0.000 description 1
- 241000283984 Rodentia Species 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 235000013402 health food Nutrition 0.000 description 1
- 230000008676 import Effects 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 239000012286 potassium permanganate Substances 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000003307 slaughter Methods 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 239000012086 standard solution Substances 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 238000012795 verification Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
- G01N33/6848—Methods of protein analysis involving mass spectrometry
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2430/00—Assays, e.g. immunoassays or enzyme assays, involving synthetic organic compounds as analytes
Definitions
- the present invention relates to a method of determining the quality of muskrat musk collected from muskrat. More specifically, the present invention relates to a method of determining the quality of muskrat must collected from muskrat which comprises measuring the content of the three components of normuscone, civetone and dihydrocivetone from the muskrat musk with a GC-MC.
- the muskrat (Ondatra zibethicus) is a mammalian which belongs to order Rodentia and family Muridae, is oval-shaped, has a body length of about 35cm and a tail length of 25cm and weighs about lkg.
- Muskrats are common near marsh and lake which is full of weed. They act from spring to late autumn and have a limited action in winter. They are herbivorous, but they also eat fish or aquatic animals. They practice monogamy and have a fine breeding.
- muskrat musk Since muskrat musk has a similar pharmacological effect similar to that of the musk from musk deer, it has a high potential to be developed as a medicinal raw material to replace the musk from a musk deer. Also, it's use in the cosmetic and perfume industry is rapidly increasing. As discussed above, muskrat is an animal which has a high potential to be developed as a new animal source of livestock industry.
- Muscone which is a major component of musk fragrance.
- Muscone has a chemical formula of C H O, is a r & 16 30 colorless and viscous oily liquid, has a molecular weight of 238 and a boiling point of
- muskrat musk is digested at 33O 0 C. It is insoluble in water, but soluble in alcohol and is stable in acid, alkaline and potassium permanganate.
- the major components of muskrat musk are normuscone which is a standard material of musk, civetone which is a standard material of civet and dihydrocivetone which is contained in muskrat musk in the highest amount.
- Muskrat musk has been developed to replace musk and its use as a raw material of medicines, health food supplements, cosmetics and perfumes is rapidly increasing. Accordingly, establishment of a method of assaying the quality of muskrat musk is urgently needed to rectify the distribution system.
- an object of the present invention is to provide a method of determining the quality of muskrat musk collected from muskrat based on the three components of normuscone, civetone and dihydrocivetone.
- the present invention provides a method of determining the quality of muskrat musk collected from muskrat (Ondatra zibethicus) which comprises the steps of [12] (a) measuring the content of normuscone from the muskrat musk to identify whether it is over 0.5% by weight based on the total weight of muskrat musk; at the same time or sequentially [13] (b) measuring the content of civetone in muskrat musk to identify whether it is over
- muskrat Ondatra zibithicus
- muskrat musk is treated with one or more organic solvents selected from ethanol, acetone and methanol for an ultrasonic extraction, the exact volume of extract is adjusted by the organic solvent and the extract is filtered to prepare a specimen to be assayed.
- organic solvents selected from ethanol, acetone and methanol
- the amount of organic solvent is 10 to 50% by weight of 100% by weight of muskrat musk.
- the amount is less than the lower limit, the extraction of muscone or all the major components is difficult.
- the ultrasonic extraction is carried out, but not limited to, for 10 to 30 minutes.
- the volume of extract is adjusted with the organic solvent. For example, as described in Example 1, Ig of muskrat musk is put into 50ml volumetric flask, and then about 45ml of organic solvent is added to the flask. After extracting, the final volume of the extract is exactly adjusted to 50ml for injection before assaying the extract with analysis apparatus.
- Filtration with a 0.45D membrane is preferably carried out to remove the substances which are insoluble to the organic solvent and impurities.
- GC-MS gas chromatography-mass spectrometry
- the temperature program carries out, but not limited to, as shown in Examples below, the following: holding the temperature of 7O 0 C for 3 minutes, raising it up to 28O 0 C at a ratio of 15°C/minutes and holding the temperature of 28O 0 C for 15 minutes.
- the quality and authenticity of muskrat musk can be easily and accurately determined by measuring the contents of the three components of normuscone, civetone and dihydrocivetone in muskrat musk and presenting certain specific content values for the three components as a standard for the determination of quality.
- FIG. 1 is a graphic diagram showing the analysis chart (Retention time 10.59) of the standard normuscone according to an embodiment of the present invention.
- FIG. 2 is a graphic diagram showing the analysis chart (Retention time for dihy- drocivetone standard: 11.95, Retention time for civetone standard: 12.01) of the standards civetone and dihydrocivetone according to an embodiment of the present invention.
- FIG. 3 is a graphic diagram showing the analysis chart (Retention time for normuscone: 10.59, Retention time for dihydrocivetone: 11.95, Retention time for civetone: 12.01) of the three components according to an embodiment of the present invention.
- FIG. 4 is a flow chart showing the process for the preparation of specimens for the analysis of the major components from muskrat musk in order. Best Mode for Carrying Out the Invention
- a gas chromatography-mass spectrometry (GC-MS) was used.
- the GC-MS was carried out by using HP-6890 for gas chromatography, HP-5973 for mass spectrometry and FFAP column with a temperature program.
- the temperature program carried out the following: holding the temperature of 7O 0 C for 3 minutes, raising it up to 28O 0 C at a ratio of 15°C/minutes and holding the temperature of 28O 0 C for 15 minutes.
- Example 1 Preparation of test liquid from muskrat musk for quality inspection
- the quality inspection of 10 kinds of muskrat musk collected from muskrat was carried out by measuring the content of normuscone, civetone and dihydrocivetone.
- the quality and authenticity of muskrat musk can be easily and accurately detremined by measuring the contents of the three components of normuscone, civetone and dihydrocivetone from muskrat must.
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- Bioinformatics & Cheminformatics (AREA)
- Bioinformatics & Computational Biology (AREA)
- Chemical & Material Sciences (AREA)
- Urology & Nephrology (AREA)
- Immunology (AREA)
- Biomedical Technology (AREA)
- Hematology (AREA)
- Cell Biology (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Food Science & Technology (AREA)
- Biotechnology (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
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Abstract
Disclosed herein is a method of determining the quality of muskrat musk collected from muskrat, wherein the contents of normuscone, civetone and dihydrocivetone in the muskrat musk are measured by a GC-MS. According to the present invention, the quality and authenticity of muskrat musk can be easily and accurately determined by measuring the contents of the three components of normuscone, civetone and dihydrocivetone in muskrat musk and presenting certain specific content values for the three components as a standard for the determination of quality.
Description
Description
QUALITY DETERMINATION METHOD OF MUSKRAT MUSK
OBTAINED FROM MUSKRAT
Technical Field
[1] The present invention relates to a method of determining the quality of muskrat musk collected from muskrat. More specifically, the present invention relates to a method of determining the quality of muskrat must collected from muskrat which comprises measuring the content of the three components of normuscone, civetone and dihydrocivetone from the muskrat musk with a GC-MC. Background Art
[2] The muskrat (Ondatra zibethicus) is a mammalian which belongs to order Rodentia and family Muridae, is oval-shaped, has a body length of about 35cm and a tail length of 25cm and weighs about lkg. Muskrats are common near marsh and lake which is full of weed. They act from spring to late autumn and have a limited action in winter. They are herbivorous, but they also eat fish or aquatic animals. They practice monogamy and have a fine breeding. Female muskrats breed from the 4 month to the 9' month and have 2 or 3 litters a year.
[3] Muskrat has a high potential to be developed as a new animal source of livestock industry. Particularly, male muskrats have two sachets and produce 5 to 8g per year. Muskrat musk has a similar pharmacological effect to that of the musk from a musk deer and, thus, has a high potential to be developed as a material to replace the musk from a musk deer.
[4] Up to date, most of natural musk has been obtained from musk deer and used as a medicinal raw material. However, in a treaty of CITES (Convention on International Trade in Endangered Species), musk deer is classified into a threatened animal and designated as a first class animal for protection, and thus the slaughter and breeding of musk deer for harvesting musk is prohibited. Thus, civet obtained from civet cat is utilized as a medicinal raw material, but the obtainable amount is extremely small and, since civet was known as a source of SARS, it has been now slaughtered and disinfected.
[5] Since muskrat musk has a similar pharmacological effect similar to that of the musk from musk deer, it has a high potential to be developed as a medicinal raw material to replace the musk from a musk deer. Also, it's use in the cosmetic and perfume industry is rapidly increasing. As discussed above, muskrat is an animal which has a high potential to be developed as a new animal source of livestock industry.
[6] The use of must in the medicine and fragrance material industries is increasing at
home. However, supply of musk depends on import and artificial synthesis. Further, since musk is expensive and the verification of quality and authenticity is difficult, problems happen frequently. Therefore, developing the replacement which has a similar pharmacological effect is needed above all things.
[7] The standard material for assaying the quality of musk is muscone which is a major component of musk fragrance. Muscone has a chemical formula of C H O, is a r & 16 30 colorless and viscous oily liquid, has a molecular weight of 238 and a boiling point of
3280C, and is digested at 33O0C. It is insoluble in water, but soluble in alcohol and is stable in acid, alkaline and potassium permanganate. [8] The major components of muskrat musk are normuscone which is a standard material of musk, civetone which is a standard material of civet and dihydrocivetone which is contained in muskrat musk in the highest amount. Muskrat musk has been developed to replace musk and its use as a raw material of medicines, health food supplements, cosmetics and perfumes is rapidly increasing. Accordingly, establishment of a method of assaying the quality of muskrat musk is urgently needed to rectify the distribution system.
Disclosure of Invention
Technical Problem [9] The present inventors have found that the authenticity of muskrat musk quality can be estimated by presenting the basis for the contents of the three components of normuscone, civetone and dihydrocivetone. [10] Therefore, an object of the present invention is to provide a method of determining the quality of muskrat musk collected from muskrat based on the three components of normuscone, civetone and dihydrocivetone.
Technical Solution [11] To accomplish the object, the present invention provides a method of determining the quality of muskrat musk collected from muskrat (Ondatra zibethicus) which comprises the steps of [12] (a) measuring the content of normuscone from the muskrat musk to identify whether it is over 0.5% by weight based on the total weight of muskrat musk; at the same time or sequentially [13] (b) measuring the content of civetone in muskrat musk to identify whether it is over
0.1% by weight based on the total weight of muskrat musk; at the same time or sequentially [14] (c) measuring the content of dihydrocivetone in muskrat musk to identify whether it is over 0.8% by weight based on the total weight of muskrat musk; at the same time or sequentially; and
[15] (d) identifying the object which is satisfied with all of the requirements in the three steps as a genuine product.
[16]
[17] Hereinafter, the present invention is further described in detail.
[18] First, the contents of normuscone, civetone and dihydrocivetone of muskrat must from muskrat (Ondatra zibithicus) are measured simultaneously or sequentially. To accurately measure the contents, preferably muskrat musk is treated with one or more organic solvents selected from ethanol, acetone and methanol for an ultrasonic extraction, the exact volume of extract is adjusted by the organic solvent and the extract is filtered to prepare a specimen to be assayed.
[19] Preferably, the amount of organic solvent is 10 to 50% by weight of 100% by weight of muskrat musk. When the amount is less than the lower limit, the extraction of muscone or all the major components is difficult.
[20] In the aspect of extraction, it is preferred that the ultrasonic extraction is carried out, but not limited to, for 10 to 30 minutes. Next, the volume of extract is adjusted with the organic solvent. For example, as described in Example 1, Ig of muskrat musk is put into 50ml volumetric flask, and then about 45ml of organic solvent is added to the flask. After extracting, the final volume of the extract is exactly adjusted to 50ml for injection before assaying the extract with analysis apparatus.
[21] Filtration with a 0.45D membrane is preferably carried out to remove the substances which are insoluble to the organic solvent and impurities.
[22] In the aspect of work convenience, it is preferred to use a gas chromatography-mass spectrometry (GC-MS) for the measurement of the contents of normuscone, civetone and dihydrocivetone. The GC-MS is carried out, but not limited to, as defined in Examples below, by using HP-6890 for gas chromatography, HP-5973 for mass spectrometry and FFAP column with a temperature program.
[23] The temperature program carries out, but not limited to, as shown in Examples below, the following: holding the temperature of 7O0C for 3 minutes, raising it up to 28O0C at a ratio of 15°C/minutes and holding the temperature of 28O0C for 15 minutes.
[24] As shown in FIG. 4, in case that the contents of normuscone, civetone and dihydrocivetone are more than 0.5% by weight, more than 0.1% by weight and more than 0.8% by weight, respectively, the tested muskrat must is genuine.
Advantageous Effects
[25] According to the present invention, the quality and authenticity of muskrat musk can be easily and accurately determined by measuring the contents of the three components of normuscone, civetone and dihydrocivetone in muskrat musk and presenting certain specific content values for the three components as a standard for
the determination of quality.
Brief Description of the Drawings
[26] FIG. 1 is a graphic diagram showing the analysis chart (Retention time 10.59) of the standard normuscone according to an embodiment of the present invention.
[27] FIG. 2 is a graphic diagram showing the analysis chart (Retention time for dihy- drocivetone standard: 11.95, Retention time for civetone standard: 12.01) of the standards civetone and dihydrocivetone according to an embodiment of the present invention.
[28] FIG. 3 is a graphic diagram showing the analysis chart (Retention time for normuscone: 10.59, Retention time for dihydrocivetone: 11.95, Retention time for civetone: 12.01) of the three components according to an embodiment of the present invention.
[29] FIG. 4 is a flow chart showing the process for the preparation of specimens for the analysis of the major components from muskrat musk in order. Best Mode for Carrying Out the Invention
[30] To analyze the content of each component, a gas chromatography-mass spectrometry (GC-MS) was used. The GC-MS was carried out by using HP-6890 for gas chromatography, HP-5973 for mass spectrometry and FFAP column with a temperature program. The temperature program carried out the following: holding the temperature of 7O0C for 3 minutes, raising it up to 28O0C at a ratio of 15°C/minutes and holding the temperature of 28O0C for 15 minutes.
[31] Ig of the standard normuscone was purchased from Woorichemtech Corporation
(Korea). 200mg of normuscone was put into a volumetric flask. After adjusting the volume with chloroform, it was used as a standard solution. ID of the solution was injected and the content was calculated on the basis of area. The result is shown in FIG. 1.
[32] The standard civetone was obtained from Dr. Ehrenstorfer GmbH (Germany). As for ID injection of civetone at a concentration of 100D/ml, the content was calculated on the basis of area. The result is shown in FIG. 2.
[33] The content of dihydrocivetone in muskrat musk was calculated in term of the same amount of the standard civetone. The result is shown in FIG. 2.
[34] The analysis was repeated three times by using the above standards. As a result of the analysis, as shown in FIG. 3, the numerical values are 10.59 for normuscone, 11.95 for dihydrocivetone and 12.01 for civetone, which was calculated based on the following equation:
[35] [Equation 1]
[36] (Area of specimen X Amount of standard / Area of standard) X 100
[37] In terms of the total weight of muskrat musk, the numerical values correspond to more than 0.5% of normuscone, more than 0.1% of civetone and more than 0.8% of di- hydrocivetone in muskrat musk. [38] From the above result, it is induced that the three components have to be comprised in an amount over the above content in muskrat musk. Mode for the Invention
[39] Example 1 : Preparation of test liquid from muskrat musk for quality inspection [40] The quality inspection of 10 kinds of muskrat musk collected from muskrat (muskrat bred in Pusan University attached farm, muskrat purchased from China for a research use, muskrat bred at a muskrat-run in Jochiwon) was carried out by measuring the content of normuscone, civetone and dihydrocivetone.
[41] As depicted in FIG. 4, Ig of muskrat musk was put into 50ml volumetric flask, and then about 45ml of ethanol was added to the flask. After ultrasonic extraction for 20 minutes, the final volume of the extract is exactly adjusted to 50ml. The volume- adjusted extract was filtered through 0.45D membrane (Millipore Inc., USA) to thereby obtain a test liquid.
[42] According to the same GC-MS method as that described in the above Example, the contents of normuscone, civetone and dihydrocivetone were measured. The result is given in Table 1.
[43] Table 1
[44]
Industrial Applicability
[45] According to the present invention, the quality and authenticity of muskrat musk can be easily and accurately detremined by measuring the contents of the three components of normuscone, civetone and dihydrocivetone from muskrat must.
Claims
[1] A method of determining the quality of muskrat musk collected from muskrat (
Ondatra zibethicus) which comprises the steps of
(a) measuring the content of normuscone in muskrat musk to identify whether it is over 0.5% by weight based on the total weight of muskrat musk; simultaneously or sequentially
(b) measuring the content of civetone in muskrat musk to identify whether it is over 0.1% by weight based on the total weight of muskrat musk; simultaneously or sequentially
(c) measuring the content of dihydrocivetone in muskrat musk to identify whether it is over 0.8% by weight based on the total weight of muskrat musk; simultaneously or sequentially; and
(d) identifying the object which is satisfied with all of the requirements in the three steps as a genuine product.
[2] The method of claim 1, wherein the measurement in steps (a), (b) and (c) is carried out by a gas chromatography - Mass spectrometry (GC-MS).
[3] The method of claim 1, wherein muskrat musk used in steps (a), (b) and (c) is a liquid which is prepared by treating the muskrat musk with one or more organic solvents selected from ethanol, acetone and methanol for a ultrasonic extraction, adjusting the volume of extract and filtering the volume-adjusted extract through a membrane.
[4] The method of claim 3, wherein the organic solvent is used in an amount of 10 to
50% by weight based on 100% by weight of muskrat musk.
[5] The method of claim 3, wherein the ultrasonic extraction is carried out for 10 to
30 minutes.
[6] The method of claim 3, wherein the pore size of membrane for the membrane filtration is about 0.45D.
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| KR10-2006-0129062 | 2006-12-16 | ||
| KR20060129062 | 2006-12-16 |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101160217B1 (en) | 2009-01-23 | 2012-06-26 | 부산대학교 산학협력단 | A method of purification for improving quality of musk obtained from Ondatra zibethicus |
| CN102914597A (en) * | 2011-08-02 | 2013-02-06 | 漳州片仔癀药业股份有限公司 | Quality testing method for fingerprint of herbal medicine musk |
| CN108037216A (en) * | 2018-01-15 | 2018-05-15 | 北京林业大学 | A kind of method that definite woods musk deer takes the fragrant time |
| CN113984917A (en) * | 2021-03-01 | 2022-01-28 | 天津中医药大学 | Method for detecting volatile chemical components in Weichang' an pills |
-
2007
- 2007-12-17 WO PCT/KR2007/006585 patent/WO2008075864A1/en active Application Filing
Non-Patent Citations (4)
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