WO2008068480A3 - Method for identifying substrates of transporter proteins expressed in the lung - Google Patents

Method for identifying substrates of transporter proteins expressed in the lung Download PDF

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Publication number
WO2008068480A3
WO2008068480A3 PCT/GB2007/004647 GB2007004647W WO2008068480A3 WO 2008068480 A3 WO2008068480 A3 WO 2008068480A3 GB 2007004647 W GB2007004647 W GB 2007004647W WO 2008068480 A3 WO2008068480 A3 WO 2008068480A3
Authority
WO
WIPO (PCT)
Prior art keywords
lung
agent
identifying
proteins expressed
transporter proteins
Prior art date
Application number
PCT/GB2007/004647
Other languages
French (fr)
Other versions
WO2008068480A2 (en
Inventor
Paul Andersson
Lena Gustavsson
Christina Eriksson
Original Assignee
Astrazeneca Ab
Astrazeneca Uk Ltd
Paul Andersson
Lena Gustavsson
Christina Eriksson
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Astrazeneca Ab, Astrazeneca Uk Ltd, Paul Andersson, Lena Gustavsson, Christina Eriksson filed Critical Astrazeneca Ab
Publication of WO2008068480A2 publication Critical patent/WO2008068480A2/en
Publication of WO2008068480A3 publication Critical patent/WO2008068480A3/en

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6872—Intracellular protein regulatory factors and their receptors, e.g. including ion channels
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Cell Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Toxicology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

The present invention relates to an assay method for identifying an agent that is taken up and retained intracellularly in lung tissue comprising the steps of: (a) contacting a test sample comprising a lung transporter protein with an agent that acts on an intracellular target in the lung; (b) identifying an agent that is a substrate for the lung transporter protein; and (c) measuring the retention time of the agent in the lung tissue, wherein a retention time of at least 0.5 hours is indicative of an agent that is retained in the lung tissue.
PCT/GB2007/004647 2006-12-06 2007-12-05 Method for identifying substrates of transporter proteins expressed in the lung WO2008068480A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US86873506P 2006-12-06 2006-12-06
US60/868,735 2006-12-06

Publications (2)

Publication Number Publication Date
WO2008068480A2 WO2008068480A2 (en) 2008-06-12
WO2008068480A3 true WO2008068480A3 (en) 2008-12-18

Family

ID=39144297

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2007/004647 WO2008068480A2 (en) 2006-12-06 2007-12-05 Method for identifying substrates of transporter proteins expressed in the lung

Country Status (1)

Country Link
WO (1) WO2008068480A2 (en)

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005098444A2 (en) * 2003-07-03 2005-10-20 Xenoport, Inc. Monocarboxylate transporters expressed in cancer cells

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005098444A2 (en) * 2003-07-03 2005-10-20 Xenoport, Inc. Monocarboxylate transporters expressed in cancer cells

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
GRONEBERG DAVID A ET AL: "Molecular mechanisms of pulmonary peptidomimetic drug and peptide transport.", AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY, vol. 30, no. 3, March 2004 (2004-03-01), pages 251 - 260, XP002473765, ISSN: 1044-1549 *
HERMANN KOEPSELL ET AL: "Polyspecific Organic Cation Transporters: Structure, Function, Physiological Roles, and Biopharmaceutical Implications", PHARMACEUTICAL RESEARCH, KLUWER ACADEMIC PUBLISHERS-PLENUM PUBLISHERS, NE, vol. 24, no. 7, 1 May 2007 (2007-05-01), pages 1227 - 1251, XP019507238, ISSN: 1573-904X *
HORVATH GABOR ET AL: "Epithelial organic cation transporters ensure pH-dependent drug absorption in the airway.", AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY JAN 2007, vol. 36, no. 1, 17 August 2006 (2006-08-17), pages 53 - 60, XP002472543, ISSN: 1044-1549 *
LIPS KATRIN SUSANNE ET AL: "POLYSPECIFIC CATION TRANSPORTERS MEDIATE LUMINAL RELEASE OF ACETYLCHOLINE FROM BRONCHIAL EPITHELIUM", AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY, AMERICAN LUNG ASSOCIATION, NEW YORK, NY, US, vol. 33, no. 1, July 2005 (2005-07-01), pages 79 - 88, XP009082702, ISSN: 1044-1549 *
MINDERMAN HANS ET AL: "VX-710 (biricodar) increases drug retention and enhances chemosensitivity in resistant cells overexpressing P-glycoprotein, multidrug resistance protein, and breast cancer resistance protein.", CLINICAL CANCER RESEARCH : AN OFFICIAL JOURNAL OF THE AMERICAN ASSOCIATION FOR CANCER RESEARCH 1 MAR 2004, vol. 10, no. 5, 1 March 2004 (2004-03-01), pages 1826 - 1834, XP002472838, ISSN: 1078-0432 *
NAVE ET AL: "Formation of fatty acid conjugates of ciclesonide active metabolite in the rat lung after 4-week inhalation of ciclesonide", PULMONARY PHARMACOLOGY AND THERAPEUTICS, ACADEMIC PRESS, NEW YORK, NY, US, vol. 18, no. 6, December 2005 (2005-12-01), pages 390 - 396, XP005078759, ISSN: 1094-5539 *
OHASHI R ET AL: "Molecular and physiological evidence for multifunctionality of carnitine/organic cation transporter OCTN2.", MOLECULAR PHARMACOLOGY FEB 2001, vol. 59, no. 2, February 2001 (2001-02-01), pages 358 - 366, XP002472541, ISSN: 0026-895X *

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Publication number Publication date
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