WO2008067651B1 - Gender selection with the use of antibodies - Google Patents

Gender selection with the use of antibodies

Info

Publication number
WO2008067651B1
WO2008067651B1 PCT/CA2007/002172 CA2007002172W WO2008067651B1 WO 2008067651 B1 WO2008067651 B1 WO 2008067651B1 CA 2007002172 W CA2007002172 W CA 2007002172W WO 2008067651 B1 WO2008067651 B1 WO 2008067651B1
Authority
WO
WIPO (PCT)
Prior art keywords
sperm
epitope polypeptide
antibody
species
chromosome
Prior art date
Application number
PCT/CA2007/002172
Other languages
French (fr)
Other versions
WO2008067651A1 (en
Inventor
Lionel Filion
Azam Akhavien-Mohammadi
Original Assignee
Biotech Inc Ab
Lionel Filion
Azam Akhavien-Mohammadi
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biotech Inc Ab, Lionel Filion, Azam Akhavien-Mohammadi filed Critical Biotech Inc Ab
Publication of WO2008067651A1 publication Critical patent/WO2008067651A1/en
Publication of WO2008067651B1 publication Critical patent/WO2008067651B1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P15/00—Drugs for genital or sexual disorders; Contraceptives
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/22—Immunoglobulins specific features characterized by taxonomic origin from camelids, e.g. camel, llama or dromedary

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Public Health (AREA)
  • Reproductive Health (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Endocrinology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Animal Behavior & Ethology (AREA)
  • Engineering & Computer Science (AREA)
  • General Chemical & Material Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

Epitope polypeptides, corresponding to antigenic regions of hydrophilic sequences selected from within mammalian gene Y-chromosome protein sequences, have been prepared and used to generate antibodies in vivo. The antibodies have been found to bind preferentially to the Y-chromosomal sperm component in semen. The binding has been found sufficient to decrease motility of such Y-chromosomal sperm component leading to substantial increases in female offspring when using the treated semen in artificial insemination.

Claims

38AMENDED CLAIMS received by the International Bureau on 26 May 2008 (26.05.2008)WHAT IS CLAIMED IS:
1. Epitope polypeptide corresponding to antigenic regions of hydrophilic protein sequences, selected from within mammalian gene Y-chromosome protein sequences.
2. The epitope polypeptide of claim 1 , wherein the Y-chromosome protein sequences are selected from genes from the group having accession numbers D30811 , AB027133, BC074923 and G49470.
3. The epitope polypeptide of claimi , selected to be common to more than one mammalian species.
4r The epitope polypeptide of claim 3, wherein the species are porcine and bovine.
5. The epitope polypeptide of claim 1 , comprising an amino acid sequence selected from the group 1 , 4, 5, 6 and 9 in Table 2.
6. The epitope polypeptide of claim 1 , comprising an amino acid sequence comprising one of the underlined sequences in Table 2.
7. Antibody to the epitope polypeptide of claim 1 ,
8. The antibody of claim 7, selected to react with Y-chromosomal sperm from more than one species.
9. A composition for artificial insemination comprising sperm and antibody of claim 7 or 8 selected to bind to the surface of only the Y-chromosomal sperm component. 39
10. A method of selecting epitope polypeptides from Y-chromosome genes comprising:
obtaining an H-Y protein sequence from a male chromosome of a mammalian species of interest; determining at least one hydrophilic region of the sequence; and preparing epitope polypeptide corresponding to the hydrophilic region.
11. The method of claim 1G followed by immunizing at least one mammalian species with at least one prepared epitope polypeptide to generate corresponding antibodies and recovering the antibodies.
12. The method of claim 11 including the additional step of selecting antibody able to react with sperm of at least two species.
13. A method of treating sperm to increase the incidence of female offspring therefrom, comprising contacting the sperm with at least one antibody recovered according to claim 11.
14. A method of decreasing motility of Y-chromosomal sperm comprising treating the sperm with antibody of claim 7.
15. A pharmaceutical composition comprising as the active ingredient the antibody of claim 7 or 8, together with a carrier therefor.
PCT/CA2007/002172 2006-12-04 2007-12-04 Gender selection with the use of antibodies WO2008067651A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CA002568512A CA2568512A1 (en) 2006-12-04 2006-12-04 Gender selection with the use of antibodies
CA2,568,512 2006-12-04

Publications (2)

Publication Number Publication Date
WO2008067651A1 WO2008067651A1 (en) 2008-06-12
WO2008067651B1 true WO2008067651B1 (en) 2008-07-31

Family

ID=39491607

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CA2007/002172 WO2008067651A1 (en) 2006-12-04 2007-12-04 Gender selection with the use of antibodies

Country Status (2)

Country Link
CA (1) CA2568512A1 (en)
WO (1) WO2008067651A1 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2361967A1 (en) * 2010-02-26 2011-08-31 Assistance Publique - Hôpitaux de Paris Gametes separation methods, compositions and uses thereof
AU2015219008B2 (en) * 2014-02-19 2020-10-01 University Of Tennessee Research Foundation Antibody for skewing sex ratio and methods of use thereof
KR101916959B1 (en) 2015-07-13 2018-11-08 김동구 Antibody for sperm sexing and use thereof
CN111566203A (en) * 2017-12-04 2020-08-21 克罗莫依安私人有限公司 Including materials and methods for gender selection
US11760793B2 (en) 2020-12-18 2023-09-19 University Of Tennessee Research Foundation Antibody for skewing sex ratio and methods of use thereof

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK0894498T3 (en) * 1997-07-01 2006-08-28 Vlp Watertown Ltd Partnership Method for determining the sex of mammalian offspring

Also Published As

Publication number Publication date
WO2008067651A1 (en) 2008-06-12
CA2568512A1 (en) 2008-06-04

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