WO2008030995A3 - Use of novel pepc to produce succinate - Google Patents

Use of novel pepc to produce succinate Download PDF

Info

Publication number
WO2008030995A3
WO2008030995A3 PCT/US2007/077806 US2007077806W WO2008030995A3 WO 2008030995 A3 WO2008030995 A3 WO 2008030995A3 US 2007077806 W US2007077806 W US 2007077806W WO 2008030995 A3 WO2008030995 A3 WO 2008030995A3
Authority
WO
WIPO (PCT)
Prior art keywords
pepc
novel
succinate
produce succinate
engineered bacteria
Prior art date
Application number
PCT/US2007/077806
Other languages
French (fr)
Other versions
WO2008030995A8 (en
WO2008030995A2 (en
Inventor
Ka-Yiu San
George N Bennett
Mary Lou Harrison
Original Assignee
Rice University
Ka-Yiu San
George N Bennett
Mary Lou Harrison
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Rice University, Ka-Yiu San, George N Bennett, Mary Lou Harrison filed Critical Rice University
Publication of WO2008030995A2 publication Critical patent/WO2008030995A2/en
Publication of WO2008030995A3 publication Critical patent/WO2008030995A3/en
Publication of WO2008030995A8 publication Critical patent/WO2008030995A8/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/44—Polycarboxylic acids
    • C12P7/46—Dicarboxylic acids having four or less carbon atoms, e.g. fumaric acid, maleic acid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

A Leuconostoc mesenteroides phosphoenolpyruvate carboxylase enzyme has been identified that improves succinate production in engineered bacteria. Expression of the novel PEPC enzyme by engineered bacteria increases production and conversion of carbon to product is improved. Methods are described for making succinate using bacterial cultures expressing L. mesenteroides PEPC.
PCT/US2007/077806 2006-09-06 2007-09-06 Use of novel pepc to produce succinate WO2008030995A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US82471706P 2006-09-06 2006-09-06
US60/824,717 2006-09-06

Publications (3)

Publication Number Publication Date
WO2008030995A2 WO2008030995A2 (en) 2008-03-13
WO2008030995A3 true WO2008030995A3 (en) 2008-10-23
WO2008030995A8 WO2008030995A8 (en) 2009-07-16

Family

ID=39158070

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2007/077806 WO2008030995A2 (en) 2006-09-06 2007-09-06 Use of novel pepc to produce succinate

Country Status (1)

Country Link
WO (1) WO2008030995A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7569380B2 (en) 2004-12-22 2009-08-04 Rice University Simultaneous anaerobic production of isoamyl acetate and succinic acid
WO2011163128A1 (en) 2010-06-21 2011-12-29 William Marsh Rice University Engineered bacteria produce succinate from sucrose

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
LIN ET AL.: "Effect of Sorghum Vulgare Phosphoenolpyruvate Carboxylase and Lactococcus Lactis Pyruvate Carboxylase Coexpression of Succinate Production in Mutant Strains of Escherichia Coli", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 67, June 2005 (2005-06-01), pages 515 - 523, XP019331828 *

Also Published As

Publication number Publication date
WO2008030995A8 (en) 2009-07-16
WO2008030995A2 (en) 2008-03-13

Similar Documents

Publication Publication Date Title
MX2009010224A (en) Microorganisms and methods for production of 1,2-propanediol and acetol.
WO2007130521A3 (en) Fermentive production of four carbon alcohols
WO2008130437A3 (en) Modified cyanobacteria
WO2008133161A1 (en) Method for production of acidic substance having carboxyl group
WO2009064200A3 (en) Novel bacteria and methods of use thereof
WO2010080407A3 (en) Methods for increasing hydrolysis of cellulosic material
NZ589632A (en) Production of butanediol by anaerobic microbial fermentation
WO2007002136A3 (en) Differentiation of primate pluripotent stem cells to cardiomyocyte-lineage cells
UA103363C2 (en) Method for enzymatic production of alcohols with four carbon atoms
EP2017332A4 (en) Microorganism capable of producing l-amino acid, and process for production of l-amino acid
WO2008143704A3 (en) Engineered microorganisms for producing n-butanol and related methods
WO2007055984A3 (en) Enzyme and methodology for the treatment of a biomass
WO2008021141A3 (en) Re-engineering bacteria for ethanol production
EP1760143A4 (en) Succinic acid-producing bacterium and process for producing succinic acid
NZ597774A (en) Bacterial expression of an artificial gene for the production of cross reactive material 197 and its derivatives
MY158628A (en) Fermentation product containing equol-producing microorganism having maintained equol-producing ability, and method for producing same
WO2009135898A3 (en) Fermentation of a lignocellulose-containing material
IL186224A0 (en) Fermented protein product
WO2007143117A3 (en) Differentiation of primate pluripotent cells to hepatocyte-lineage cells
WO2009067471A3 (en) Processes of producing fermentation products
WO2008093829A1 (en) Method for production of l-amino acid
WO2011115439A3 (en) Microorganism with improved production of 5'-xanthosine monophosphate and 5'-guanine monophosphate, and production method of 5'-xanthosine monophosphate and 5'-guanine monophosphate using same
WO2009005123A1 (en) INTERFERON-β PRODUCTION PROMOTER, AND METHOD FOR PRODUCTION THEREOF
WO2008030995A3 (en) Use of novel pepc to produce succinate
WO2008105201A1 (en) Method for production of vinegar, and vinegar produced by the method

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 07842010

Country of ref document: EP

Kind code of ref document: A2

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 07842010

Country of ref document: EP

Kind code of ref document: A2