WO2008025316A3 - Sample preparation method for quantitative analysis of samples containing amino acids - Google Patents

Sample preparation method for quantitative analysis of samples containing amino acids Download PDF

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Publication number
WO2008025316A3
WO2008025316A3 PCT/DE2007/001339 DE2007001339W WO2008025316A3 WO 2008025316 A3 WO2008025316 A3 WO 2008025316A3 DE 2007001339 W DE2007001339 W DE 2007001339W WO 2008025316 A3 WO2008025316 A3 WO 2008025316A3
Authority
WO
WIPO (PCT)
Prior art keywords
amino acids
quantitative analysis
sample preparation
samples containing
containing amino
Prior art date
Application number
PCT/DE2007/001339
Other languages
German (de)
French (fr)
Other versions
WO2008025316A2 (en
Inventor
Bernd Kastenholz
Bjoern Thiele
Original Assignee
Forschungszentrum Juelich Gmbh
Bernd Kastenholz
Bjoern Thiele
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Forschungszentrum Juelich Gmbh, Bernd Kastenholz, Bjoern Thiele filed Critical Forschungszentrum Juelich Gmbh
Publication of WO2008025316A2 publication Critical patent/WO2008025316A2/en
Publication of WO2008025316A3 publication Critical patent/WO2008025316A3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/42—Low-temperature sample treatment, e.g. cryofixation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6806—Determination of free amino acids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/286—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
    • G01N2001/2866—Grinding or homogeneising
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N1/4055—Concentrating samples by solubility techniques
    • G01N2001/4061—Solvent extraction

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Biochemistry (AREA)
  • Hematology (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • Cell Biology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Sampling And Sample Adjustment (AREA)

Abstract

The invention relates to a sample preparation method for quantitative analysis of samples containing amino acids, comprising the following steps: a) comminution and homogenisation of the sample, b) extraction of amino acids, c) separation of the liquid extraction phase containing the amino acids from possible remaining solids, insofar as there are solids remaining, d) separation of the amino acids from the extraction medium, e) transfer of the amino acids to an organic phase and f) drying the organic phase, with, at least in steps a), b) and c), cooling being carried out. The cooling is preferably carried out using liquid nitrogen as refrigerant.
PCT/DE2007/001339 2006-08-29 2007-07-28 Sample preparation method for quantitative analysis of samples containing amino acids WO2008025316A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE200610040444 DE102006040444A1 (en) 2006-08-29 2006-08-29 Sample preparation procedure for the quantitative analysis of samples containing amino acids
DE102006040444.0 2006-08-29

Publications (2)

Publication Number Publication Date
WO2008025316A2 WO2008025316A2 (en) 2008-03-06
WO2008025316A3 true WO2008025316A3 (en) 2008-06-05

Family

ID=39032332

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DE2007/001339 WO2008025316A2 (en) 2006-08-29 2007-07-28 Sample preparation method for quantitative analysis of samples containing amino acids

Country Status (2)

Country Link
DE (1) DE102006040444A1 (en)
WO (1) WO2008025316A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB201310214D0 (en) * 2013-06-07 2013-07-24 Medical Res Council Separation and Analysis Systems and Methods

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3856471A (en) * 1968-08-05 1974-12-24 Nasa Amino acid analysis

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2142767B1 (en) * 1998-07-14 2000-12-01 Consejo Superior Investigacion METHOD FOR DETERMINING THE TOTAL TIME OF THE MANUFACTURING PROCESS OF CURED WHITE PORK HAM (GLUJAM).
CZ9900770A3 (en) * 1999-03-04 2000-10-11 Petr Ing. Drsc. Hušek Process for preparing samples for analysis of amino acids and kit for this preparation
DE19914792A1 (en) * 1999-03-31 2000-10-05 Max Planck Gesellschaft Production of transgenic plants by transforming metabolically defective plants with DNA that can complement the defect, eliminating need for e.g. antibiotic resistance markers
US20040176293A1 (en) * 2002-12-18 2004-09-09 Wyeth Methods for screening, treating and diagnosing inflammatory bowel disease and compositions thereof

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3856471A (en) * 1968-08-05 1974-12-24 Nasa Amino acid analysis

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
"EZ:Faast(TM) User's Manual: Amino acid analysis of protein hydrolysates by GC-FID or GC-NPD", 25 June 2004, PHENOMENEX INC., TORRANCE, CA, US, XP002472775 *
BATTAGLIA A ET AL: "High-performance liquid chromatographic analysis of physiological amino acids in human brain tumors by pre-column derivatization with phenylisothiocyanate", JOURNAL OF CHROMATOGRAPHY B : BIOMEDICAL APPLICATIONS, ELSEVIER SCIENCE PUBLISHERS, NL, vol. 730, no. 1, 25 June 1999 (1999-06-25), pages 81 - 93, XP004172059, ISSN: 0378-4347 *
MUHLING J ET AL: "Quantitative determination of free intracellular amino acids in single human polymorphonuclear leucocytes - Recent developments in sample preparation and high-performance liquid chromatography", JOURNAL OF CHROMATOGRAPHY B : BIOMEDICAL APPLICATIONS, ELSEVIER SCIENCE PUBLISHERS, NL, vol. 728, no. 2, 28 May 1999 (1999-05-28), pages 157 - 166, XP004169145, ISSN: 0378-4347 *

Also Published As

Publication number Publication date
WO2008025316A2 (en) 2008-03-06
DE102006040444A1 (en) 2008-03-13

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