WO2008013454A2 - Immunogenic compounds and protein mimics - Google Patents
Immunogenic compounds and protein mimics Download PDFInfo
- Publication number
- WO2008013454A2 WO2008013454A2 PCT/NL2007/050374 NL2007050374W WO2008013454A2 WO 2008013454 A2 WO2008013454 A2 WO 2008013454A2 NL 2007050374 W NL2007050374 W NL 2007050374W WO 2008013454 A2 WO2008013454 A2 WO 2008013454A2
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- WO
- WIPO (PCT)
- Prior art keywords
- amino acid
- bromomethyl
- acid sequence
- scaffold
- immunogenic
- Prior art date
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Classifications
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/59—Follicle-stimulating hormone [FSH]; Chorionic gonadotropins, e.g.hCG [human chorionic gonadotropin]; Luteinising hormone [LH]; Thyroid-stimulating hormone [TSH]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P35/00—Antineoplastic agents
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
- C07K14/71—Receptors; Cell surface antigens; Cell surface determinants for growth factors; for growth regulators
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
- C07K14/715—Receptors; Cell surface antigens; Cell surface determinants for cytokines; for lymphokines; for interferons
- C07K14/7158—Receptors; Cell surface antigens; Cell surface determinants for cytokines; for lymphokines; for interferons for chemokines
Definitions
- the invention relates to the fields of biology and immunology.
- Immunogenic compounds are used in a wide variety of applications, such as for instance vaccination programs and the production of antibodies, B cells and T cells of interest.
- an immunogenic compound is used in order to evoke an immune response in a host, which immune response is preferably a protective immune response against a disease related to the presence of an antigen of interest such as for instance a pathogen or a tumor.
- Such immunogenic compounds typically comprise a peptide sequence that is wholly or in part derived from said antigen of interest.
- Immunogenic compounds are also widely used for obtaining antibodies, B cells and/or T cells of interest.
- Non-human animals are immunized with an immunogenic compound, where after antibodies, B cells and/or T cells are harvested from the animal for further use.
- mAbs monoclonal antibodies
- the production of monoclonal antibodies (mAbs) starting from an immunogenic compound of interest is an important application.
- the benefits of mAbs is their ability to target specific cells or chemical mediators that could be involved in disease causation. This specificity confers certain clinical advantages on mAbs over more conventional treatments while offering patients an effective, well-tolerated therapy option with generally low side effects.
- immunization not always yields the desired effect.
- immunization against self-antigens is difficult because a host's immune system is in principle not directed against self-antigens.
- An immune response against a self-antigen is however desired in various cases, for instance when a self-antigen is present on tumor cells.
- An immune response against a self-antigen present on a tumor would counteract the tumor.
- tumor growth requires angiogenesis, which involves the formation of new blood vessels, in order to carry nutrients to the site of the tumor and to transport waste material from the tumor.
- Angiogenesis involves the action of endogenous growth hormones such as vascular endothelial growth factor (VEGF), also called vascular permeability factor (VPF).
- VEGF vascular endothelial growth factor
- VPF vascular permeability factor
- immunogenic compounds are often generated which are sufficient different from the self antigen in order to elicit an immune response, yet sufficiently resemble the self antigen such that an elicited immune response is also active against the self antigen.
- immunogenicity is often enhanced with a carrier, such as for instance keyhole limpet hemocyanin (KLH), and/or an adjuvant such as for instance (incomplete) Freund's adjuvant.
- KLH keyhole limpet hemocyanin
- adjuvant such as for instance (incomplete) Freund's adjuvant.
- Another problem encountered in the field of immunology is lack of immunogenic reproducibility. This means that a desired immune response is obtained in one animal, but not, or to a significantly lesser extent, in a second animal of the same species even though both animals are immunized with the same kind of immunogenic compound. This variability in immune responses between animals of the same species is not well understood. Biologic diversity between individuals is generally considered to result in differences between immune systems of different animals.
- the invention in one aspect provides a method for inducing and/or enhancing immunogenic reproducibility and/or immunogenicity of a compound, the method comprising at least in part restricting the conformation of said compound.
- immunogenicity of a compound of interest is induced and/or improved by (further) restricting the conformation of said compound. Immunogenicity is even improved when said compound already has a rather limited conformation, for instance due to a linkage to a carrier. Even then, further restricting the conformation significantly improves immunogenicity. If a compound with (further) restricted conformation is administered to an animal, an immune response against a certain antigen of interest is elicited which is stronger as compared to the situation wherein the same kind of compound with a less restricted conformation is administered to the same kind of animal. The extent of an immune response is for instance determined by measuring antibody titer in a blood sample of an immunized animal.
- an immunogenic reproducibility of an immunogenic compound is induced and/or enhanced by (further) restricting the conformation of said compound.
- a method of the invention it has become possible to generate immunogenic compounds which, when administered to a group of animals of the same species, are capable of inducing the same kind of immune response in a larger part of said group of animals as compared to current immunogenic compounds.
- an immunogenic compound according to the invention with restricted conformation is capable of eliciting a comparable immune response in a higher percentage of a group of animals of the same species as compared to the same kind of compound with a less restricted conformation. Animal to animal variation is reduced.
- an animal capable of eliciting an immune response against an immunogenic compound is encompassed by the term "animal".
- An animal preferably comprises a mammal.
- said animal comprises a human individual.
- said animal comprises a non-human animal, for instance when an immune response is induced in order to harvest antibodies, T cells and/or B cells.
- the conformation of a compound is defined as the number of possible spatial arrangements of a compound. In view of rotation about single covalent bonds, free compounds often adopt many different conformations. Restricting the conformation of a compound involves limiting the number of possible spatial arrangements, thereby forcing the compound to spend more time in a certain conformational state.
- Immunogenic reproducibility is defined herein as the chance that the same kind of immune response which is obtained in one animal is also obtained in a second animal of the same species when both animals are immunized with the same immunogenic compound. If immunogenic reproducibility is enhanced, a larger percentage of a group of animals will exhibit the same kind of immune response. By the same kind of immune response is meant that the specificity and extent of the immune responses are comparable.
- an immune response in a first animal is comparable to an immune response in a second animal if the antibody titers of both animal differ less than hundredfold, preferably less than fifty fold, most preferably less than thirty fold. If antibody titers are given as Log values, an immune response in a first animal is comparable to an immune response in a second animal if the antibody titers of both animals differ less than 2.0, preferably less than 1.5.
- Immunogenicity of a compound is defined as the capability of a compound of eliciting an immune response specifically directed against the compound itself and/or, preferably, against a molecule of interest.
- Said molecule of interest preferably comprises a proteinaceous molecule.
- said compound is capable of eliciting antibodies which strongly bind to, and neutralize the biological activity of, said molecule of interest.
- a proteinaceous molecule is defined as a molecule comprising amino acid residues bound to each other via a peptide bond. Said molecule may comprise one or several non-amino acid moieties.
- a compound's capability of eliciting an immune response specifically directed against a (proteinaceous) molecule of interest is called cross-reactivity.
- a method according to the invention is preferably used for inducing and/or enhancing cross- reactivity of a compound.
- a preferred embodiment therefore provides a method for inducing and/or enhancing cross-reactivity of a compound, the method comprising at least in part restricting the conformation of said compound.
- An immunogenic compound is preferably derived from a proteinaceous molecule of interest, meaning that said immunogenic compound comprises an amino acid sequence which is at least 50% homologous to said proteinaceous molecule of interest.
- An immunogenic compound comprising an amino acid sequence which is at least 50% homologous to a proteinaceous molecule of interest is preferably capable of inducing an immune response which is specific for said proteinaceous molecule of interest.
- An immunogenic compound according to the invention thus preferably comprises an amino acid sequence comprising a sequence which is at least 50% homologous to at least part of the amino acid sequence of a proteinaceous molecule of interest, said part having a length of at least 8 amino acid residues.
- said amino acid sequence comprises a sequence which is at least 60%, preferably at least 70%, more preferably at least 80%, more preferably at least 90%, more preferably at least 95%, most preferably at least 97% homologous to at least part of the amino acid sequence of a proteinaceous molecule of interest, said part having a length of at least 8 amino acid residues.
- Such immunogenic compound is particularly suitable for eliciting an immune response against a proteinaceous molecule of interest.
- said immunogenic compound is capable of eliciting a stronger immune response against said proteinaceous molecule of interest as compared to a situation wherein an animal is immunized with said proteinaceous molecule itself.
- TDK-Alascan involves substitution of an original amino acid residue by alanine.
- a replacement net mapping method an original amino acid residue is replaced by any other amino acid residue.
- a plurality of molecules is generated, wherein different amino acid residues are replaced, either by alanine or by any other amino acid residue.
- immunogenicity preferably comprising cross-reactivity
- a molecule with a desired characteristic is subsequently identified and/or isolated. Said molecule is either used for immunization, or further optimized in another round of substitution and selection method. Of course, other optimization procedures are applicable as well.
- an amino acid sequence is used that is at least 50%, preferably at least 60%, more preferably at least 70, 80, 90 or 95%, homologous to a non immunodominant site of a proteinaceous molecule of interest.
- Immunodominant sites are sites against which an immune response is primarily directed after immunization with a proteinaceous molecule of interest. Such immunodominant sites are for instance easily accessible. However, it is often desired to elicit antibodies against another specific site which is not immunodominant, such as for instance a receptor binding site. In that case the use of a peptide derived from said specific non- immunodominant site is preferred.
- This embodiment is for instance particularly suitable for inducing and/or enhancing an immune response against a receptor binding site of G Protein-Coupled Receptors (GPCR's), such as for instance the chemokine receptors CCR5 and/or CXCR4.
- GPCR's G Protein-Coupled Receptors
- an immunogenic compound with restricted conformation is capable of inducing and/or enhancing an immune response in an animal against a proteinaceous molecule of interest.
- an at least partial protective and/or curative immune response is elicited.
- a protective immune response means that an animal which has been immunized will suffer less - if at all - from a disease related to the presence of said proteinaceous molecule of interest. For instance, if said proteinaceous molecule is present on a pathogen, said animal will suffer less, preferably not suffer at all, from an infection by said pathogen after the animal has been immunized.
- said immunized animal will be better capable of preventing and/or counteracting (growth of) said tumor. As a result the animal will suffer less, or not at all, from a tumor-related disease.
- a curative immune response means that an animal which is already suffering from a disease related to the presence of a proteinaceous molecule of interest will be better capable of counteracting (symptoms of) said disease.
- the conformation of a compound is restricted in various ways.
- the conformation is restricted by the formation of a linkage at at least two different sites of said compound.
- Each of said at least two different sites of said compound is either bound to another compound, such as a scaffold, or to another site within said compound, thus forming an internal bond.
- a linkage is preferably formed at a site outside an epitope. This means that amino acid residues which are part of an epitope of interest that is to be recognized by antibodies and/or T cell receptors generated by the animal's immune system are preferably not used for forming a linkage, because this will often diminish recognition of said epitope by an animal's immune system.
- such epitope of interest comprises an epitope capable of eliciting an immune response against a proteinaceous molecule of interest. If amino acids of such epitope of interest are used for formation of a linkage, the resulting compound will often be less — if at all — capable of eliciting an immune response which is capable of subsequently recognizing a proteinaceous molecule of interest.
- linkage When a linkage is inside an epitope it should preferably be formed between amino acid positions that are not crucial or heavily involved in antibody-binding.
- Crucial amino acids of an epitope are amino acids whose presence is required for eliciting an immune response which is capable of recognizing a proteinaceous molecule of interest.
- a skilled person is well capable of determining experimentally which amino acid residues are suitable for formation of a linkage while preserving epitope recognition, and which amino acid residues should not be used.
- the conformation of a compound is restricted by the formation of at least one internal bond within said compound.
- an immunogenic compound according to the invention comprising two internal bonds is provided.
- a compound according to the invention is attached to a scaffold and/or carrier.
- An immunogenic compound which is bound to a scaffold and/or carrier and which comprises an internal bond is most preferably provided.
- such immunogenic compound is particularly suitable for inducing and/or enhancing immunogenic reproducibility and/or immunogenicity of a compound. It has been found that such compound is often even better capable of inducing and/or enhancing immunogenic reproducibility and/or immunogenicity, as compared to a compound having other types of linkages, such as for instance two internal bonds.
- An immunogenic compound which is bound to a scaffold and/or carrier and which comprises an internal bond is therefore particularly preferred.
- Said compound is preferably attached to a scaffold and/or carrier via at least two linkages because this particularly limits the conformation of said compound. Therefore, an immunogenic compound is preferably provided which is bound to a scaffold and/or carrier via at least two linkages and which additionally comprises at least one internal bond.
- the invention provides the insight that even though the conformation of current immunogenic compounds bound to a carrier or scaffold is already more restricted as compared to free compounds, a significant improvement in immunogenicity and immunogenic reproducibility is still obtained if the conformation of said scaffold-bound compound is further restricted, preferably by the formation of a second linkage.
- Said second linkage is preferably an internal bond, because the formation of an internal bond within a scaffold-bound compound particularly enhances immunogenicity and immunogenic reproducibility of said compound. Since a compound's conformation is already restricted when the compound is bound to a carrier or scaffold, an additional linkage - be it an internal bond or a linkage to another compound - would not be expected to have a significant effect. According to the invention, however, the formation of a second linkage does provide a significant improvement.
- a method according to the invention is particularly applicable for optimizing the three-dimensional structure of an immunogenic compound which is capable of inducing an immune response against a proteinaceous molecule of interest.
- the conformation of such immunogenic compound is restricted in order to force the compound to spend more time in a conformational state which closely resembles the three-dimensional structure of the corresponding epitope in said proteinaceous molecule.
- the native three-dimensional structure of an epitope is more closely mimicked.
- a method according to the invention is therefore particularly suitable for optimizing immunogenic compounds derived from epitopes with a specific three-dimensional structure in a proteinaceous molecule.
- epitopes examples include non-linear epitopes and/or epitopes that are present in a specific three-dimensional structure found in proteins, such as for instance a loop structure.
- a preferred example of such loop structure is a beta-hairpin which often occurs between two antiparallel beta-strands. Beta-hairpins are often relatively easily accessible. As a result, immune responses are often directed against epitopes present in beta-hairpin sequences.
- Loop structures are also present in members of the cystine- knot superfamily. These members have an unusual arrangement of six cysteines linked to form a "cystine-knot" conformation, shown in Figure 4A.
- the cystine-knot structure comprises 2 distorted beta-hairpin loops "above” the knot and a single beta- hairpin loop "below” the knot. Immunodominant epitopes are often found within these loops.
- the three-dimensional structure of epitopes present in such loop structures are preferably mimicked with a method according to the invention.
- the conformation of a peptide sequence derived from a hairpin-loop is preferably restricted via at least two linkages such that the conformation of said peptide sequence closely resemble the native three-dimensional structure of the hairpin-loop.
- immunogenic compound comprises an amino acid sequence which is at least 50%, preferably 60%, more preferably 70, 75, 80, 85, 90 and/or 95%, homologous to a part of an amino acid sequence of a proteinaceous molecule of interest, said part having a length of at least 8 amino acid residues, wherein said part comprises a nonlinear epitope of said proteinaceous molecule and/or wherein said part comprises a sequence of at least 6 amino acid residues, preferably at least 8 amino acid residues, which is present in a loop, preferably a hairpin loop, of said proteinaceous molecule.
- the three-dimensional structure of a native epitope is mimicked by restricting the conformation of an immunogenic compound, comprising a sequence at least partly derived from said epitope, with a method according to the invention.
- the locations of at least two linkages are chosen such that the resulting conformation of the immunogenic compound resembles the native conformation of said epitope in said proteinaceous molecule of interest.
- the position of an internal bond is chosen such that the conformation of the resulting compound closely resembles the native conformation of an epitope of said proteinaceous molecule of interest.
- the position of a linkage between said immunogenic compound and a scaffold is chosen such that the conformation of the resulting compound closely resembles the native conformation of an epitope of said proteinaceous molecule of interest.
- the conformation of an immunogenic compound according to the invention is also influenced by the type of scaffold that is used, since the size and the shape of the scaffold influence the overall structure of the immunogenic compound.
- a skilled person is well capable of designing an immunogenic compound according to the invention with a conformation closely resembling the native conformation of an epitope of a proteinaceous molecule of interest.
- the location of at least two linkages are chosen such that a conformation is obtained which closely resembles the native conformation of an epitope of said proteinaceous molecule of interest.
- a linkage is preferably not located within an epitope of interest, because such linkage would disturb the conformation and/or accessibility of the epitope.
- the kind of scaffold and the location where the scaffold is linked to an amino acid sequence of the immunogenic compound are chosen such that a conformation is obtained which closely resembles the native conformation of an epitope of said proteinaceous molecule of interest.
- a compound with optimal immunogenic properties is preferably selected. This is for instance exemplified in example 1.
- example 1 is shown how an optimal conformation is determined by varying the location of an internal bond.
- the method outlined in this example is not limiting the invention. It is also possible to produce several compounds with different kinds of scaffolds, either linked at identical or different locations of an amino acid sequence, and to experimentally determine the immunogenicity and/or immunogenic reproducibility of the resulting compounds.
- an immunogenic compound encompasses any kind of compound capable of eliciting an immune response in a host.
- an immunogenic compound according to the invention comprises an amino acid sequence.
- the invention is now further described for the preferred embodiments wherein immunogenic compounds comprising an amino acid sequence are used.
- an immunogenic compound comprising an amino acid sequence is preferably restricted by attaching the amino acid sequence to a carrier or scaffold, either directly or indirectly, for instance via a linker, and by the formation of at least one internal bond within said amino acid sequence.
- Said internal bond preferably comprises a disulphide bond (also called an SS-bridge) because disulphide bonds are selectively formed between free cysteine residues without the need to protect other amino acid side chains.
- disulphide bonds are easily formed by incubating in a basic environment.
- a disulphide bond is formed between two cysteine residues, since their sulfhydryl groups are readily available for binding.
- an SS-bridge within an amino acid sequence is easily regulated by regulating the location of free cysteine residues.
- said cysteines are located around the first and last amino acid position of the amino acid sequence, in order to optimally restrict the conformation of the amino acid sequence.
- an immunogenic compound of the invention is also suitable for restricting the conformation of an immunogenic compound of the invention.
- Se-Se diselenium bonds are used.
- An advantage of diselenium bonds is the fact that these bonds are reduction insensitive.
- immunogenic compounds comprising a diselenium bond are better capable of maintaining their conformation under reducing circumstances, for instance present within an animal body.
- a diselenium bond is preferred when a free SH-group is present within the immunogenic compound, which SH-group is for instance used for a subsequent coupling reaction to a carrier.
- Such free SH-group is not capable of reacting with a diselenium bond.
- a metathese reaction is used in order to form an internal bond.
- a metathese reaction two terminal CC-double bonds or triple bonds are connected by means of a Ru-catalysed rearrangement reaction.
- Such terminal CC-double or CC-triple bonds are for instance introduced into a peptide either via alkylation of the peptide NH-groups, for instance with allyl bromide or propargyl bromide, or via incorporating a non-natural amino acid with an alkenyl- or alkynyl-containing side chain into the peptide.
- a metathese reaction does not occur spontaneously, but is performed with a Grubbs-catalyst.
- an internal bond is formed using Br-SH cyclisation.
- an SH moiety of a free cysteine is coupled to a BrAc-moiety which is preferably present at the N-terminus of the peptide or at a lysine (RNH2) side chain.
- a CC ⁇ H-side chain of an aspartate or glutamate residue is coupled to the NEb-side chain of a lysine residue.
- This way an amide bond is formed.
- It is also possible to form an internal bond by coupling the free C ⁇ 2H-end of a peptide to the free NH2-end of the peptide, thereby forming an amide-bond.
- Alternative methods for forming an internal bond within an amino acid sequence are available, which methods are known in the art.
- an internal bond is formed anywhere within an immunogenic amino acid sequence, as long as the primary, secondary and tertiary sequence of at least one epitope of interest is essentially maintained.
- a linkage is formed between any one of the ten N-terminal and ten C-terminal amino acid residues of the amino acid sequence.
- a linkage is formed between any one of the six N-terminal and six C-terminal amino acid residues, preferably between any one of the four N-terminal and four C-terminal amino acid residues, of the amino acid sequence.
- the sites that are suitable for the formation of an internal bond are dependent on the location of the epitope(s) of interest.
- a linkage is formed between the first and the last amino acid residue of an immunogenic amino acid sequence.
- An immunogenic compound according to the invention preferably comprises an amino acid sequence attached to a scaffold and/or a carrier because a scaffold and/or carrier enhances immunogenicity.
- said amino acid sequence comprises an internal bond.
- An immunogenic compound comprising an amino acid sequence bound to a scaffold and/or carrier, wherein said amino acid sequence comprises at least one internal bond is therefore also herewith provided.
- An immunogenic compound is preferably attached to a scaffold and/or carrier via at least two linkages in order to further limit flexibility of the amino acid sequence.
- an amino acid sequence comprising at least one internal bond is attached to a scaffold or carrier via at least two linkages. This way, immunogenicity and/or immunogenic reproducibility is particularly enhanced.
- said scaffold comprises a (hetero)aromatic molecule with at least a first and a second reactive group as disclosed in WO 2004/077062, preferably a (hetero)aromatic molecule comprising at least two benzylic halogen substitutents.
- the two benzylic halogen substitutents are preferably used as first and second reactive group for coupling an amino acid sequence.
- An amino acid sequence is preferably coupled to a scaffold using a method according to WO 2004/077062. Briefly, a scaffold with at least a first and a second reactive group is provided.
- An amino acid sequence capable of reacting with said at least first and second reactive group is contacted with said scaffold under conditions allowing said amino acid sequence to react with said at least first and second reactive group to form at least two linkages between said scaffold and said amino acid sequence, wherein the formation of a first linkage accelerates the formation of a consecutive linkage.
- This way, conformationally constraint loop constructs are formed.
- the coupling reaction using a scaffold as disclosed in WO 2004/077062 is suitable for being performed in solution.
- An amino acid sequence is preferably coupled to a scaffold using a method according to WO 2004/077062 in an aqueous solution, thereby limiting or even avoiding the use of (toxic) organic solvents.
- Water is environment-friendly and easily removed by freeze-drying.
- many unprotected peptides are well soluble in water, as well as most salts, allowing the use of ammonium bicarbonate, one of the few volatile salts, which defines the pH to a slightly basic value of pH 7.8 to 8.0 in aqueous solution.
- first linkage accelerates the formation of a second linkage
- attachment of an amino acid sequence to a scaffold according to WO 2004/077062 takes place in a rapid, concerted process comprising a cascade of reactions.
- the formation of a first linkage, also referred to as (chemical) bond or connection, via a first reactive group increases the reactivity of a second reactive group, and so on, such that the activating effect is being 'handed over' from one reactive group to the next one.
- Said chemical reactions involve changes at functional groups while the molecular skeleton of the scaffold remains essentially unchanged.
- a scaffold molecule as used in WO 2004/077062 comprising at least two reactive groups is capable of reacting with an amino acid sequence such that the reactive groups of the scaffold become involved in the new linkages with the amino acid sequence while the core structure or skeleton of the scaffold does not participate directly in the coupling.
- a synthetic scaffold comprising at least two identical reactive groups is coupled to one or more immunogenic peptides.
- Said one or more immunogenic peptides are further provided with a second linkage, preferably an internal bond, either before or after coupling of the peptide(s) to the scaffold.
- Suitable peptides comprise all possible peptides capable of reacting with at least two reactive groups on a scaffold to form at least two linkages or bonds between said peptide(s) and said scaffold, which typically results in a looped or cyclic peptide on a scaffold.
- the essence of such a bond formation is charge attraction and electron movement.
- the coupling reaction between an amino acid sequence and a scaffold involves a nucleophilic substitution reaction wherein an amino acid sequence with a free nucleophilic functionality reacts with a scaffold.
- a nucleophile typically shares an electron pair with an electrophile in the process of bond formation. In other words, a nucleophile is seeking a center of electron deficiency (an atom) with which to react.
- Nucleophiles, ('nucleus-loving') can be negatively charged or uncharged, and include for example heteroatoms other than carbon bearing a lone pair, or pi electrons in any alkene or alkyne.
- Electrophiles are electrically neutral or positively charged and have some place for the electrons to go, be it an empty orbital (as in BH3) or a potentially empty orbital.
- said nucleophilic functionality comprises a thiol or sulfhydryl group.
- Thiols are effective nucleophiles for substitution at saturated carbon atoms. It is in general not difficult to provide an amino acid sequence with a nucleophilic functionality. For example, an amino acid sequence is easily functionalised with a thiol moiety by incorporating a cysteine residue in the amino acid sequence.
- the leaving group departs with the electron pair by which it was originally bound to the carbon atom.
- a scaffold is used which contains at least two leaving groups in order to facilitate the formation of at least two bonds with at least one amino acid sequence. The ease with which a leaving group departs is related to the basicity of that group; weak bases are in general good leaving groups because they are able to accommodate the electron pair effectively.
- a scaffold comprising at least two reactive groups each comprising a good leaving group is used in a method according to the invention.
- Good leaving groups are in general the conjugate bases of strong acids.
- Important leaving groups are the conjugate bases of acids with pKa values below 5.
- Particularly interesting leaving groups include halide ions such as I-, Br-, and Cl-.
- a carbon-halogen (C-X) bond in an alkyl halide is polarised, with a partial positive charge on the carbon and a partial negative charge on the halogen.
- the carbon atom is susceptible to attack by a nucleophile (a reagent that brings a pair of electrons) and the halogen leaves as the halide ion (X-), taking on the two electrons from the C-X bond. Therefore, in one embodiment an amino acid sequence is coupled to a reactive group of a scaffold, the reactive group comprising a carbon atom susceptible to attack by a nucleophile wherein said reactive group comprises a carbon- halogen bond.
- a scaffold comprising at least two of such reactive groups is used to react with a di-SH functionalised peptide as nucleophile.
- a method for obtaining an immunogenic compound comprising a scaffold with at least one looped peptide structure, said method comprising contacting said scaffold with at least one peptide, wherein said scaffold comprises a halogenoalkane, where after the coupled peptide is provided with a second linkage, preferably an internal bond.
- the peptide is provided with the second linkage, preferably an internal bond, before it is coupled to the halogenoalkane.
- Halogenoalkanes are compounds containing a halogen atom (fluorine, chlorine, bromine or iodine) joined to one or more carbon atoms in a chain.
- a halogen atom fluorine, chlorine, bromine or iodine
- dihaloscaffolds comprising two halogen atoms
- tri-and tetrahaloscaffolds for the synthesis of conformationally constraint compounds, like for example peptide constructs consisting of one or more looped peptide segments bound to said scaffold, wherein the looped peptide segments are further provided with a second linkage, preferably with an internal bond.
- a good leaving group is electronegative to polarize the carbon atom, it is stable with an extra pair of electrons once it has left, and is polarizable, to stabilize the transition state.
- iodine all of the halogens are more electronegative than carbon.
- Chlorine and bromine have fairly similar electronegativities and polarize the bond with the carbon fairly equally.
- Bromide ion is also more polarizable due to its larger size.
- an immunogenic peptide is preferably bound to a scaffold comprising at least two Cl atoms, more preferably bound to a scaffold comprising at least one Cl atom and at least one Br atom and even more preferably bound to a scaffold comprising at least two Br atoms.
- a conformationally restricted amino acid sequence is bound to a scaffold which comprises an allylic system.
- an allylic system there are at least three carbon atoms, two of which are connected through a carbon-carbon double bond.
- the formation of a bond or linkage between a scaffold and an immunogenic peptide occurs via an allylic substitution reaction.
- An allylic substitution reaction refers to a substitution reaction occurring at position 1 of an allylic system, the double bond being between positions 2 and 3.
- the incoming group becomes attached to the same atom 1 as the leaving group, or the incoming group becomes attached at the relative position 3, with movement of the double bond from 2/3 to 1/2.
- the reaction rate of allylic substitutions is very high, because the allyl cation reaction intermediate, a carbon atom ' bearing a positive charge attached to a doubly-bonded carbon, is unusually stable. This is because an allylic cation is a resonance hybrid of two exactly equivalent structures. In either of the contributing structures, there is an empty p orbital with the pi cloud of the electron-deficient carbon. Overlap of this empty p orbital with the pi cloud of the double bond results in delocalisation of the pi electrons, hereby providing electrons to the electron-deficient carbon and stabilizing the cation.
- a scaffold comprising at least two allylic halogen atoms. Due to electrondelocalisation, allyl halides tend to undergo ionization very readily to produce a carbocation and a halide ion, such that breaking the carbon halide bond is rapid.
- a carbon-oxygen double bond i.e. a carbonyl group
- resonance structures are formed which contribute to stabilization of a carbocation.
- a scaffold comprises two or more reactive groups comprising the structure-C(O)-CH2- halogen.
- a nucleophilic substitution reaction the structure of the substrate plays just as important role as the nature of the leaving group. For example, if a nucleophile attacks the backside of the carbon, the reaction proceeds unhindered if the leaving group is bonded to a methyl, where the hydrogens leave enough surface to attack the carbon. As that carbon becomes more substituted, larger groups hinder the path the nucleophile must take to displace the leaving group. For these reasons, it is also advantageous that a scaffold comprise at least two halomethyl groups.
- a scaffold comprises a conjugated polyene, also known as aromatic compound, or arene, which is provided with at least two reactive groups.
- An aromatic compound is flat, with cyclic clouds of delocalised pi electrons above and below the plane of the molecule.
- a molecular scaffold is used which comprises at least two benzylic halogen substituents, like for instance halomethyl groups. Suitable examples include, but are not limited, to di (halomethyl) benzene, tri (halomethyl) benzene or tetra (halomethyl) benzene and derivatives thereof.
- a preferred embodiment of the invention therefore provides a method wherein an amino acid sequence is bound via at least two linkages to a scaffold comprising a (he tero) aromatic molecule with at least two benzylic halogen substituents and wherein the conformation of said amino acid sequence is further restricted, preferably via an internal bond.
- Said internal bond preferably comprises an internal disulfide bond.
- Said amino acid sequence is preferably bound to the (he tero) aromatic molecule using two free cysteine thiols of the amino acid sequence.
- said scaffold is a halomethylarene, preferably selected from the group consisting of bis(bromomethyl)benzene, tris(bromomethyl)benzene and tetra(bromomethyl)benzene, or a derivative thereof. More preferably said scaffold is selected from the group consisting of ortho-, meta- and para- dihaloxyleen and 1,2,4,5 tetra halodurene.
- Said scaffold most preferably comprises meta- 1,3- bis(bromomethyl)benzene (m-T2), ortho- l,2-bis(bromomethyl)benzene (o-T2), para- l,4-bis(bromomethyl)benzene (p-T2), meta-l,3-bis(bromomethyl)pyridine (m-P2), 2,4,6-tris(bromomethyl)mesitylene (T3), meta-l,3-bis(bromomethyl)-5-azidobenzene (m-T3-N3) and/or 1,2,4,5 tetrabromodurene (T4).
- the invention thus provides a method for inducing and/or enhancing the immunogenicity and/or immunogenic reproducibility of an amino acid sequence
- a method for inducing and/or enhancing the immunogenicity and/or immunogenic reproducibility of an amino acid sequence comprising coupling said amino acid sequence, which amino acid sequence preferably comprises two free cysteine thiols, to a (hetero)aromatic molecule with at least two benzylic halogen substituents, preferably a halomethylarene, more preferably bis(bromomethyl)benzene, tris(bromomethyl)benzene and/or tetra(bromomethyl)benzene or a derivative thereof, and forming at least one internal bond within said amino acid sequence.
- Said internal bond is either formed before or after coupling of the amino acid sequence to the scaffold.
- said internal bond is formed after the coupling reaction has been performed.
- Said amino acid sequence is preferably coupled to the (hetero)aromatic molecule via at least two linkages in order to particularly limit the conformation of said amino acid sequence.
- an amino acid sequence is provided with an internal bond and coupled to m-T2, o-T2, p-T2, m-P2, T3, m-T3-N3 and/or T4.
- Suitable molecular scaffolds also include polycyclic aromatic compounds with smaller or larger ring structures.
- suitable scaffolds are not limited to hydrocarbons.
- a heterocyclic aromatic scaffold - a cyclic molecule with at least one atom other than carbon in the ring structure, most commonly nitrogen, oxygen or sulfur - is also suitable. Examples include pyrrole, furan, thiophene, imidazole, oxazole, thiazole, pyrazole, -3-pyrroline, pyridine, pyrimidine and derivatives thereof.
- Preferred heterocyclic aromatic scaffolds include but are not limited to those comprising at least two halomethyl groups.
- a preferred scaffold is meta-dibromo-pyridine .
- an amino acid sequence is coupled to a scaffold that is based on or which consists of multiple ring aromatic structures, such as fused-ring aromatic compounds. Two aromatic rings that share a carbon-carbon bond are said to be fused.
- Suitable fused-ring aromatic scaffolds include for example naphthalene, anthracene or phenanthrene and derivatives thereof, provided that they contain at least two reactive groups.
- a fused-ring aromatic scaffold comprises at least two reactive groups wherein each group contains a highly reactive benzylic halogen atom, for example a halomethyl group.
- a scaffold comprises a multi-ring or fused ring structure, for instance a scaffold wherein aromatic, e. g. benzene, rings are connected directly via a carbon-carbon bond is used. Alternatively, said rings are connected via a linker comprising at least one atom. Examples of suitable scaffolds are given in Figures 1-3. From a commercial point of view, a scaffold according to the invention is preferably commercially available at a relatively low cost and obtainable in large quantities. For example, the dibromoscaffold 1,3-bis (bromomethyl) benzene is currently being sold for only around 5 euro per gram.
- a peptide molecule for use in a method according to the invention is a synthetic peptide, for instance obtained using standard peptide synthesis procedures.
- Synthetic peptides are obtained using various procedures known in the art. These include solid phase peptide synthesis (SPPS) and solution phase organic synthesis (SPOS) technologies. SPPS is a quick and easy approach to synthesize peptides and small proteins.
- the C-terminal amino acid is for instance attached to a cross-linked polystyrene resin via an acid labile bond with a linker molecule. This resin is insoluble in the solvents used for synthesis, making it relatively simple and fast to wash away excess reagents and by-products.
- Suitable peptides comprise peptides of various length.
- oligopeptides ranging from as small as 3 amino acids in length to polypeptides of 27 residues have been successfully used in a method provided.
- Coupling of an amino acid sequence to a scaffold according to WO 2004/077062 allows for the use of unprotected amino acid sequences.
- the only functionality that cannot be present in unprotected form is the cysteine SH, as it will be involved in the coupling reaction.
- an amino acid sequence is used which, besides two free cysteine residues for coupling to a scaffold, comprises at least two more additional cysteine (Cys)residues.
- the Acm or StBu group is not removed during the course of the normal TFA deprotection-cleavage reaction but requires oxidative (I2/VitC) treatment in case of Acm group, or reductive treatment (BME (excess) or 1,4-DTT (excess)) in case of StBu group to give the reduced sulfhydryl form of the peptide, which can either be used directly or subsequently oxidized to the corresponding cystinyl peptide.
- a peptide which contains at least one Cys derivative, such as Cys(Acm) or
- Cys(StBu) to allow selective unmasking of a Cys-thiol group.
- Selective unmasking of a Cys-thiol group allows to make the Cys-thiol group available for reacting at a desired moment, such as following completion of the coupling reaction between a scaffold and a peptide. This is for instance very attractive for forming an internal bond within the peptide after the peptide has been bound to a scaffold.
- a linear peptide is synthesized, comprising two unprotected Cys residues and two protected Cys derivatives at other positions.
- the di-SH functionalized peptide is coupled to a (hetero)aromatic scaffold comprising at least two benzylic halogen substituents, resulting in the structural fixation of a looped peptide on the scaffold. Subsequently, the two Cys-derivatives are unmasked and used for forming an internal disulfide bridge.
- a compound comprising an amino acid sequence bound to a scaffold and/or a carrier via at least two linkages, wherein said amino acid sequence comprises at least one internal bond.
- said internal bond comprises an SS-bridge, preferably between two cysteine residues of said amino acid sequence because their sulfhydryl groups are readily available for binding.
- Said cysteines are preferably located around the first and last amino acid position of the amino acid sequence in order to at least partially avoid the formation of free rotating peptide ends.
- Said compound preferably comprises an immunogenic compound and/or a protein mimic.
- a non-limiting example of a protein mimic according to the invention is a peptide that mimics the binding properties of a protein (for instance receptor activating or receptor inhibiting).
- the sites where a linkage is formed are dependent on the position of at least one epitope of interest within said amino acid sequence. In general, a linkage is not formed at a site within an epitope sequence, because that would diminish immunogenicity.
- An immunogenic compound according to the invention preferably comprises an amino acid sequence that is bound to a scaffold via at least two linkages, because this particularly limits the conformation of said compound.
- said scaffold preferably comprises a (hetero) aromatic molecule comprising at least two benzylic halogen substitutents, preferably a halomethylarene.
- Preferred scaffolds are bis(bromomethyl)benzenes, tris(bromomethyl)benzenes and tetra(bromomethyl)benzenes, or derivatives thereof.
- An immunogenic compound according to the invention preferably comprises a scaffold selected from the group consisting of ortho-, meta- and para- dihaloxyleen and 1,2,4,5 tetra halodurene, preferably selected from the group consisting of meta-l,3-bis(bromomethyl)benzene (m-T2), ortho- l,2-bis(bromomethyl)benzene (o-T2), para-l,4-bis(bromomethyl)benzene (p-T2), meta-l,3-bis(bromomethyl)pyridine (m-P2), 2,4,6-tris(bromomethyl)mesitylene (T3), meta-l,3-bis(bromomethyl)-5-azidobenzene (m-T3-N3) and 1,2,4,5 tetrabromodurene (T4).
- m-T2 meta-l,3-bis(bromomethyl)benzene
- o-T2 ortho- l,2-bis(bromomethyl)
- An immunogenic compound according to the invention is particularly suitable for inducing and/or enhancing a desired immune response.
- an immunogenic compound according to the invention is combined with a pharmaceutically acceptable carrier, adjuvant, diluent and/or excipient in order to enhance antibody production or a humoral response.
- suitable carriers for instance comprise keyhole limpet haemocyanin (KLH), serum albumin (e.g. BSA or RSA) and ovalbumin.
- KLH keyhole limpet haemocyanin
- serum albumin e.g. BSA or RSA
- ovalbumin ovalbumin.
- suitable adjuvants oil-based and water-based, are known to a person skilled in the art.
- said adjuvant comprises Specol.
- said suitable carrier comprises a solution like for example saline.
- An immunogenic composition comprising an immunogenic compound according to the invention and a pharmaceutically acceptable carrier, adjuvant, diluent and/or excipient is therefore also provided.
- Said immunogenic composition preferably comprises a vaccine, capable of inducing a protective immune response.
- an immunogenic compound according to the invention is used for inducing and/or enhancing a curative immune response in order to treat a patient suffering from a disease.
- An immunogenic compound according to the invention for use as a medicament and/or vaccine is also herewith provided.
- Dose ranges of compounds according to the invention to be used in the prophylactic and/or therapeutic applications as described herein are designed on the basis of rising dose studies in the clinic in clinical trials for which rigorous protocol requirements exist. Typically, doses vary between 0.01-1000 ⁇ g/kg body weight, particularly about 0.1-100 ⁇ g/kg body weight.
- An immunogenic compound according to the invention preferably comprises an amino acid sequence derived from a proteinaceous molecule of interest, in order to elicit an immune response against said proteinaceous molecule of interest.
- said proteinaceous molecule of interest is selected from the group consisting of the cystine-knot family, transmembrane proteins, TNF-alpha, HGF/SF, FGF-beta, interleukins, IL-5, chemokines, G-protein-coupled receptors, CCR5,
- an immune response against any of these proteinaceous molecules of interest is preferably elicited and/or enhanced in order to prevent and/or counteract a disorder related to the presence of said proteinaceous molecule of interest.
- an immunogenic compound according to the invention for the preparation of a medicament and/or vaccine against a disorder related to the presence of a member of the cysteine-knot family, a transmembrane protein, TNF-alpha, HGF/SF, FGF-beta, an interleukin, IL-5, a chemokine, a G-protein-coupled receptor, CCR5, CXCR4, IGF, LMF, endothelin-1, VIP, CGRP, PIF, EGF, TGF-alpha, the ErbB family,
- HER1/EGF-R HER2/neu, HER3, HER4, p53, corticotropin RF, ACTH, parathyroid hormone, CCK, substance P, NPY, GRP, neurotrophine, angiotensin-2, angiogenin, angiopoietin, neurotensine, SLCLC, SARS-derived protein, HIV-derived protein, papillomavirus-derived protein and/or FMDV.
- an amino acid sequence of an immunogenic composition according to the invention is derived from a member of the cystine-knot family.
- cystine-knot Members of the cystine-knot (Cys-knot) family have an unusual arrangement of six cysteines linked to form a "cystine-knot" conformation.
- the active forms of these proteins are dimers, either homo- or heterodimers . Because of their shape, there appears to be an intrinsic requirement for the cystine-knot growth factors to form dimers. This extra level of organization increases the variety of structures built around this simple structural motif. Many members of the Cys-knot family are growth factors.
- TGF-beta2 platelet-derived growth factor
- NGF nerve growth factor
- hCG human chorionic gonadotropin
- 6 conserved cysteine residues form 3 disulphide bonds arranged in a knot-like topology.
- the two disulphide bonds between CysII and CysV ([CysII-V]) and between CysIII and CysVT ([CysIII -VI]) form a ring-like structure of 8 amino acids through which the remaining disulphide bond (between Cysl and CysIV) penetrates (see Figure 4A).
- the sulfur (S) atoms of the conserved cysteines I to Vl that are involved in the disulphide bonds are typically referred to as Sl to S6. Cystine knot domains with more than 6 cysteine residues can be found. The "extra" cysteine residues are normally used to create further disulphide bonds within the cystine knot domain or interchain disulphide bonds, during dimerisation. However, based on homology and topology it is always possible to indicate which cysteines represent the six conserved residues Cysl to CysVI (see further below).
- cystine topology differs: Cys[III-VI] penetrates a macrocyclic ring formed by Cys[I-IV] and Cys[II-V].
- cystine-knot proteins fall into 2 structural classes: growth factor type and inhibitor-like cystine knots.
- the cystine-knot growth factor superfamily is divided into subfamilies, which include the glycoprotein hormones (e.g. follicle stimulating hormone (FSH)), the transforming growth factor beta (TGF-beta) proteins (e.g. bone morphogenetic protein 4), the platelet-derived growth factor- like (PDGF-like) proteins (e.g. platelet derived growth factor A), nerve growth factors (NGF) (e.g. brain-derived neurotrophic factor) (see also Tables 1 and 2).
- FSH follicle stimulating hormone
- TGF-beta transforming growth factor beta
- PDGF-like proteins e.g. platelet derived growth factor A
- NGF nerve growth factors
- All growth factor cystine knot structures have a similar topology, with 2 distorted beta-hairpin (beta-1 and beta-3) loops "above” the knot and a single (beta- 2) loop "below” the knot.
- the beta-1 loop is formed by the stretch of amino acids between Cysl and CysII ;
- the beta-2 loop is formed by the amino acids between CysIII and CysIV and
- the beta-3 loop is formed by the amino acids between CysIV and CysV (see Figure 4A).
- the sizes of the hairpin loops i.e. the number of amino acids between the indicated cysteines) can vary significantly between family members.
- an immunogenic compound according to the present invention comprises an amino acid sequence that is capable of inducing and/or enhancing an immune response in an animal against a member of the glycoprotein hormone-beta (GLHB) subfamily, the platelet-derived growth factor (PDGF) subfamily, the transforming growth factor (TGF) subfamily, the nerve growth factor (NGF) subfamily or the glycoprotein hormone-alpha (GLHA) subfamily.
- GLHB glycoprotein hormone-beta
- PDGF platelet-derived growth factor
- TGF transforming growth factor
- NGF nerve growth factor
- GLHA glycoprotein hormone-alpha
- Said amino acid sequence preferably comprises a sequence which is at least 50%, more preferably at least 60%, more preferably at least 70%, more preferably at least 80%, more preferably at least 85%, more preferably at least 90%, more preferably at least 95%, most preferably at least 98% homologous to at least part of said cystine-knot protein family member, said part having a length of at least 8 amino acid residues.
- the higher the homology the more specifically an elicited immune response will be directed against said cystine-knot protein family member.
- These subfamilies play an important role in the formation and proliferation of many different types of cancers. Therefore, eliciting an immune response specifically directed against at least one member of these subfamilies is useful for preventing and/or counteracting this kind of diseases. Moreover, some members of these subfamilies are involved in fertility regulation (hCG, FSH).
- an immunogenic compound capable of inducing an immune response against VEGF is enhanced.
- Eliciting and/or enhancing an immune response against VEGF (in particular VEGF-A/B, VEGF-C and/or VEGF-D) counteracts (lymph)angiogenesis.
- VEGF-A/B, VEGF-C and/or VEGF-D counteracts (lymph)angiogenesis.
- VEGF-A/B, VEGF-C and/or VEGF-D counteracts (lymph)angiogenesis.
- Tumor growth requires (lymph)angiogenesis, which involves the formation of new blood (or lymphatic) vessels, in order to carry nutrients to the site of the tumor, to transport waste material from the tumor, and to spread.
- Counteracting (lymph)angiogenesis therefore hampers tumor growth and spread.
- vascular endothelial growth factor VEGF-A/B, VEGF-C and/or VEGF-D.
- VEGF-A/B, VEGF-C and/or VEGF-D vascular endothelial growth factor
- placental growth factor (PlGF) is involved in angiogenesis. Eliciting an immune response against PlGF therefore counteracts angiogenesis and, indirectly, counteracts tumor growth. Since PlGF is primarily involved in angiogenesis in tumour tissue but not, or to a significantly lower extent, in angiogenesis in normal tissue, therapy involving eliciting and/or enhancing an immune response against PlGF will particularly avoid negative side-effects.
- the immunogenicity and/or immunogenic reproducibility of an immunogenic compound capable of inducing an immune response against hCG is enhanced. hCG is often overexpressed in tumour tissue. Eliciting an immune response against hCG therefore attacks tumour tissue. Since hCG is normally only expressed during pregnancy, negative side effects are at least in part avoided in non-pregnant individuals.
- HER human epidermal growth factor receptor
- HGF/SF hepatocyte growth factor/stimulating factor
- An immune response against these proteins therefore also attacks tumor tissue.
- An immune response against VEGF, hCG, PlGF, HER and/or HGF/SF is preferably elicited with an amino acid sequence that is at least partly derived from said proteins.
- the amino acid sequences are preferably - but not necessarily - optimized using for instance a TDK-Alascan method and/or replacement net mapping. This way immunogenicity is enhanced.
- an immunogenic compound comprising an amino acid sequence comprising a sequence which is at least 50%, preferably at least 60%, more preferably at least 70%, more preferably at least 75%, more preferably at least 80%, more preferably at least 85%, more preferably at least 90%, more preferably at least 95%, most preferably at least 98%, homologous to at least part of the amino acid sequence of VEGF, hCG, PlGF, HER and/or HGF/SF, said part having a length of at least 8 amino acid residues.
- a use of said immunogenic compound for the preparation of a medicament and/or vaccine against a tumor-related disease is also provided, as well as a method for vaccinating an animal against a tumor-related disease, comprising administering to said animal a suitable dose of an immunogenic compound according to the invention, wherein said immunogenic compound comprises an amino acid sequence comprising a sequence which is at least 50%, preferably at least 60%, more preferably at least 70%, more preferably at least 75%, more preferably at least 80%, more preferably at least 85%, more preferably at least 90%, more preferably at least 95%, most preferably at least 98% homologous to at least part of the amino acid sequence of VEGF, hCG, PlGF, HER and/or HGF/SF, said part having a length of at least 8 amino acid residues.
- an immunogenic compound according to the invention comprises an amino acid sequence comprising a sequence which is at least 50%, preferably at least 60%, more preferably at least 70%, more preferably at least 75%, more preferably at least 80%, more preferably at least 85%, more preferably at least 90%, more preferably at least 95%, most preferably at least 98% homologous to at least part of the amino acid sequence of hCG, said part having a length of at least 8 amino acid residues, for the preparation of a medicament and/or vaccine for fertility regulation is also provided.
- VEGF vascular endothelial growth factor
- PlGF primarily involved in angiogenesis in tumour tissue
- VEGF VEGF-A/B, VEGF-C and VEGF-D
- angiogenesis in other (non-tumour) tissue as well.
- non-tumour-related situations it is often desired to counteract angiogenesis, for instance in case of inocular retinopathy, during which undesired angiogenesis in the eye results in loss of sight.
- an immunogenic compound comprises an amino acid sequence comprising a sequence which is at least 50%, preferably at least 60%, more preferably at least 70%, more preferably at least 75%, more preferably at least 80%, more preferably at least 85%, more preferably at least 90%, more preferably at least 95%, most preferably at least 98% homologous to at least part of the amino acid sequence of VEGF, said part having a length of at least 8 amino acid residues, for the preparation of a medicament and/or vaccine against angiogenesis.
- Another embodiment provides a method for vaccinating an animal against angiogenesis, comprising administering to said animal a suitable dose of an immunogenic compound according to the invention, wherein said immunogenic compound comprises an amino acid sequence comprising a sequence which is at least 50%, preferably at least 60%, more preferably at least 70%, more preferably at least 75%, more preferably at least 80%, more preferably at least 85%, more preferably at least 90%, more preferably at least 95%, most preferably at least 98% homologous to at least part of the amino acid sequence of VEGF, said part having a length of at least 8 amino acid residues.
- said immunogenic compound according to the invention is for instance administered to an individual in order to elicit and/or enhance an immune response against VEGF-A or VEGF-B.
- the eye is provided with anti VEGF-A or anti VEGF-B antibodies or T cells derived from the individual or a non-human animal after immunization with said immunogenic compound according to the invention.
- harvested antibodies or T cells are preferably further processed in order to adapt them to human use, using methods known in the art. For instance, the six hypervariable regions from the heavy and light chains of a non-human antibody are incorporated into a human framework sequence and combined with human constant regions.
- Hepatocyte growth factor receptor/stimulating factor is primarily involved with the formation of metastases once a tumour is present. Especially in case of prostate cancer HGF/SF plays an important role. In order to counteract the formation of metastases it is thus particularly preferred to counteract the action of HGF/SF.
- an immunogenic compound comprises an amino acid sequence comprising a sequence which is at least 50%, preferably at least 60%, more preferably at least 70%, more preferably at least 75%, more preferably at least 80%, more preferably at least 85%, more preferably at least 90%, more preferably at least 95%, most preferably at least 98% homologous to at least part of the amino acid sequence of HGF/SF, said part having a length of at least 8 amino acid residues, for the preparation of a medicament and/or vaccine against the formation of metastases during a tumor-related disease, preferably during prostate cancer.
- HER or ErbB Human epidermal growth factor receptor (HER or ErbB (or EGF-R in case of HER or ErbB).
- HER-I or neu in case of HER-2) is overexpressed by a wide variety of tumours, such as for instance breast tumor. Since HER is overexpressed by many tumors, immunization against HER thus provides a general therapy for a broad spectrum of tumor-related diseases.
- an immunogenic compound comprises an amino acid sequence comprising a sequence which is at least 50%, preferably at least 60%, more preferably at least 70%, more preferably at least 75%, more preferably at least 80%, more preferably at least 85%, more preferably at least 90%, more preferably at least 95%, most preferably at least 98% homologous to at least part of the amino acid sequence of HER, said part having a length of at least 8 amino acid residues, for the preparation of a medicament and/or vaccine against a tumor-related disease, preferably breast cancer.
- One embodiment provides a method for preparing a compound according to the invention, the method comprising:
- Said molecule preferably comprises an amino acid sequence.
- Said internal bond preferably comprises an SS-bridge. As stated before, said SS-bridge is preferably a bond between two cysteine residues, which cysteine residues are preferably located around the N-terminal and C-terminal ends of the amino acid sequence.
- said scaffold comprises a (hetero)aromatic molecule, preferably a (hetero)aromatic molecule comprising at least two benzylic halogen substitutents, more preferably a halomethylarene, said halomethylarene preferably being selected from the group consisting of bis(bromomethyl)benzene, tris(bromomethyl)benzene and tetra(bromomethyl)benzene, or a derivative thereof.
- said scaffold is selected from the group consisting of ortho-, meta- and para- dihaloxyleen and
- 1,2,4,5 tetra halodurene preferably meta-l,3-bis(bromomethyl)benzene (m-T2), ortho- l,2-bis(bromomethyl)benzene (o-T2), para-l,4-bis(bromomethyl)benzene (p-T2), meta- l,3-bis(bromomethyl)pyridine (m-P2), 2,4,6-tris(bromomethyl)mesitylene (T3), meta- l,3-bis(bromomethyl)-5-azidobenzene (m-T3-N3) or 1,2,4,5 tetrabromodurene (T4).
- m-T2 meta-l,3-bis(bromomethyl)benzene
- o-T2 ortho- l,2-bis(bromomethyl)benzene
- p-T2 para-l,4-bis(bromomethyl)benzene
- T3 2,4,6-tris(bromomethyl
- Immunogenic compounds according to the invention are particularly suitable for the production of antibodies, T cells and B cells, using a non-human animal. Further provided is therefore a method for producing antibodies, T cells and/or B cells, comprising: - immunizing a non-human animal with an immunogenic compound according to the invention and/or an immunogenic composition according to the invention, and - harvesting antibodies, T cells and/or B cells capable of specifically binding said immunogenic compound from said animal. A preferred embodiment further comprises producing monoclonal antibodies using said antibody obtained from said animal. Methods and protocols for immunizing non-human animals and harvesting antibodies, T cells and/or B cells, as well as isolating antibodies of interest and producing monoclonal antibodies, are well known in the art and need no further explanation here.
- the elicited antibodies, T cells and/or B cells are further used for human benefit.
- the genes encoding the Ig heavy and/or light chains are isolated from a harvested B cell and expressed in a second cell, such as for instance cells of a Chinese hamster ovary (CHO) cell line.
- Said second cell also called herein a producer cell, is preferably adapted to commercial antibody production. Proliferation of said producer cell results in a producer cell line capable of producing antibodies of interest.
- said producer cell line is suitable for producing compounds for use in humans.
- said producer cell line is preferably free of pathogenic agents such as pathogenic micro-organisms.
- nucleic acid encoding the T cell receptor is isolated from a harvested T cell of interest and incorporated into naive (preferably human) T cells.
- the T cells are preferably cultured in order to obtain a T cell line.
- FIG. 1 Aromatic scaffolds with ortho-, meta-, or para-positioning of two halomethyl groups.
- Hal refers to chlorine, bromo, or iodine atoms.
- Figure 2 Aromatic scaffolds with ortho-, meta-, or para-positioning of three halomethyl groups: l,2,3-tris(halomethyl)benzene and other regioisomers
- Figure 4 Schematic representation of the B3-loop of Cys-knot growth factor family members and peptidomimetics thereof.
- Panel A shows the general loop-structure of the various members of the Cys-knot protein family.
- Panel B shows the B3-loop including residues CysIV and CysV.
- Panel C shows the structural design of a peptidomimetic of the invention wherein two cysteines are introduced in the polypeptide such that covalent attachment of the polypeptide via these cysteines to a scaffold (indicated as T) induces the peptide to adopt a conformation which resembles the secondary structure of the B3-loop in the native protein
- Figure 5 Antibody responses from vaccination experiments with FSH-63 loop mT2/SS CLIPS-peptides
- Figure 7 ELISA competition studies with anti-peptide antisera obtained by immunization experiments with linear, single-constrained (T2 only) and double- constrained (T2 and SS) peptides derived from the VEGF-A 65-loop- ⁇ 6 segment.
- FIG. 8 ELISA competition studies with linear, single-constrained (T2 only) and double -constrained (T2 and SS) peptides derived from the ECL2a-loop of the CCR5 co- receptor (GPCR-family).
- Peptides were synthesized by solid-phase peptide synthesis using a 4-(2',4'- dimethoxyphenyl-Fmoc-aminomethyl)-phenoxy (RinkAmide) resin (BACHEM, Germany) on a Syro-synthesizer (MultiSynTech, Germany). All Fmoc-amino acids were purchased from Orpegen Pharma (Heidelberg, Germany) or Senn Chemicals
- N-f-Boc KW
- O- t-Bu DESTY
- N-Trt HNQ
- S-Trt C
- S(Acm) C
- N-Pbf R
- a coupling protocol using a 6.5-fold excess of HBTU/HOBt/amino acid/DIPEA (1:1:1:2) in NMP with a 30 minute activation time using double couplings was employed.
- Acetylation of peptides was performed by reacting the resin with NMP/AC2O/DIEA (10: 1:0.1, v/v/v) for 30 min at room temperature.
- the crude peptides were purified by reversed-phase high performance liquid chromatography (RP-HPLC), either on a "DeltaPack” (25x100 or 40x210 mm inner diameter, 15 urn particle size, 100 A pore size; Waters, USA) or on a "XTERRA” (19 x 100 mm inner diameter, 5 um particle size (Waters, USA) RP- 18 preparative Ci ⁇ column with a lineair AB gradient of 1-2% B/min. where solvent A was 0.05% TFA in water and solvent B was 0.05% TFA in ACN.
- RP-HPLC reversed-phase high performance liquid chromatography
- Micromass ZQ Micromass, The Netherlands
- VG Quattro II VG Organic, UK
- Amino acids are indicated by the single-letter code.
- the asterisk (*) indicates acetylation of the N-terminus and the number sign (#) amidation of the C-terminus.
- T2/SS or P2/SS-peptides Peptides, containing two free cysteines (CT) and two Acm-protected cysteines (Cs), were cyclized onto a T2/P2 CLIPS via reaction with 1.05 equivalent of the corresponding (bisbromomethyl)benzene or pyridine compound in 25% ACN/75% ammonium bicarbonate (20 mM, pH 7.8) for 3 hours at room temperature.
- CT free cysteines
- Cs Acm-protected cysteines
- the T2/P2-peptide constructs were treated with excess (10 equiv.) of I2 in a mixture of MeOH/DMSO (9:1, v/v) at 1 mM (final concentration) for 15 min.
- reaction mixtures were then diluted with 9 volumes of H2O and filtered over a RP Ci ⁇ -eartridge (Sep-Pak® Vac 3cc for HPLC- extraction, Waters Corporation, Massachusetts, USA).
- the peptide-constructs were then collected by elution with ACN/H2O (6 mL, 1: 1 v/v) followed by removal of the solvent by freeze-drying.
- the peptide-constructs were further purified by RP-HPLC, followed by treatment with an anion-exchange resin (BIO-RAD, AG 1-X8 resin, 100- 200 mesh) for 2-3 h at room temperature. Finally, the peptide-constructs were freeze- dried (3x) from ACN/milliQ-H2 ⁇ solution in order to ensure complete removal of traces of TFA and/or ammonium bicarbonate.
- 1,2- (oT2), 1,3- (mT2), l,4-(bisbromomethyl)benzene (pT2), and 1,3- bis(bromomethyl)pyridine (mP2) were purchased from Sigma-Aldrich (Zwijndrecht, The Netherlands).
- Antisera were analyzed in a FSH-binding ELISA (Greiner, PS; GDA-coating with 1 ⁇ g/mL of protein) using 2,2'-azine-di(ethylbenzthiazoline sulfonate) (ABTS) in combination with a peroxidase-labeled Goat-anti-rat serum as second antibody.
- ABTS 2,2'-azine-di(ethylbenzthiazoline sulfonate)
- commercially available anti-protein antibodies were included in the analysis as positive controls.
- FSH was purchased from Biotrend.
- the FSH-inhibiting activity of the anti-FSH anti-sera was measured in a human FSHR cell-line assay, using Yl mouse adrenal cells, stably transfected with the cDNA for the hFSHR as described by Westhoff et al. (Biol. Reprod. 1997, 56, 460-468). This assay measures the ability of anti-FSH antibodies to neutralize the hormonal (bio)activity of native FSH onto its cell-surface receptor (FSHR).
- FSHR cell-surface receptor
- Anti-FSH mAb 6602 was used as positive control. FSH was purchased from Biotrend.
- hFSH-derived peptides were synthesized and their ability to raise hFSH cross-reactive antibodies was assessed:
- the anti-FSH antibody titres obtained with a method according to the invention are significantly higher and more reproducible (2x4.6 i.s.o. 3.5/1.5) than for a corresponding singly-constrained peptide rigidified only via an mT2-CLIPS at position 61 and 74 and lacking an internal disulfide (SS) bond (compound 1).
- Table 4 and Figure 6 summarize data on neutralizing activity (NT) and ELISA-titers (ET) for hFSH of rat antisera raised against these 2 sets of peptides, that mimic the beta3-loop of FSH.
- Table 5 summarizes data on neutralizing activity (NT) and ELISA-titers (ET) for hFSH of rat antisera raised against constrained peptides that mimic the FSH beta3- loop.
- Example 1 is almost not dependent on the type of C ⁇ C-constraint (ortho/meta/para- xylyl; 2,6-dimethylpyridyl) used to fix the peptides conformation. Results are optimal with a w ⁇ e£ ⁇ -oriented xylyl or dimethylpyridyl-based linker at position 61-74, but results with the ortho/para-xylyl-based linkers are still much better (higher hFSH- ET/NT) than with lineair or single -constrained peptides. .
- Table 6 summarizes data on neutralizing activity (NT) and ELISA-titers (ET) for hFSH of rat antisera raised against doubly T2/SS-constrained peptides that mimic the FSH beta3-loop.
- a linkage is preferably formed outside an epitope, although non-essential amino acids inside an epitope can also be used.
- Table 7 summarizes data on neutralizing activity (NT) and ELISA-titers (ET) for hFSH of rat antisera raised against this set of constrained peptides that mimic the FSH beta3-loop.
- CTC and CsC double-constrained peptides derived from the beta5-loop-beta6 region of hVEGF (yet another member of the cys-knot growth factor family) to generate hVEGF-crossreactive antisera.
- CTC and CsC double-constrained peptides derived from the beta5-loop-beta6 region of hVEGF (yet another member of the cys-knot growth factor family) to block the binding of anti-VEGF mAb to surface- immobilized hVEGF.
- a binding competition experiment was carried out with the 6 antipeptide antisera 29.1-31.2 (see example 5B) in order to assess the ability of the sera to bind to hVEGF.
- the wells were incubated for 1 h at 37C with 100 uL of a 1:1 mixture of an anti-VEGF mAb (40 ng/mL) and 1 of the 6 antisera (at 1/10 dilution) in 5% horse-serum.
- the amount of anti-VEGF mAb bound to the surface-immobilized VEGF was then determined using 2,2'-azine-di(ethylbenzthiazoline sulfonate) (ABTS) in combination with a peroxidase-labeled Goat-anti-human serum as second antibody, and used as a measure for the ability of the antipeptide sera to compete for binding with the anti-VEGF mAb. Binding of anti-peptide sera to bind to VEGF and consequently the ability to block niAb-binding translates into low binding levels for the mAb.
- ABTS 2,2'-azine-di(ethylbenzthiazoline sulfonate)
- CTC and CsC double-constrained peptides derived from the dimerization arm in domain-II (CRl) of the ErbB2 (HER2/neu) protein belonging to the epidermal growth factor receptor family (EGFR) to generate ERbB2-crossreactive antisera.
- CTC and CsC double-constrained peptides derived from the extracellular domain loop-2 (ECL-2) of the CCR5-receptor protein belonging to the family of G-protein coupled receptors (GPCR' s) to mimic better the receptor protein.
- mAb 2D7 The ability of peptides to bind to mAb 2D7 was measured in ELISA, with a peptide derived from the ECL2a-loop of CCR5 immobilized to the surface. mAb 2D7 shows strong binding to this peptide in ELISA. Binding of peptides 36-39 to 2D7 in solution can now be studied in competition with binding to the surface-immobilized peptide, and translates into decreased surface binding of mAb 2D7.
- the amount of mAb 2D7 bound to the surface-immobilized constrained peptide was then determined using 2,2'-azine-di(ethylbenzthiazoline sulfonate) (ABTS) in combination with a peroxidase-labeled Rabbit-anti-mouse serum as second antibody.
- ABTS 2,2'-azine-di(ethylbenzthiazoline sulfonate)
- Double-constrained peptides derived from the ECL2-loop of receptor protein CCR5 provide much better mimics of this receptor than the corresponding lineair or single- constrained peptides, as established in a competition ELISA experiments. This will undoubtedly translate into improved ability of these peptides to generate antibodies or antisera that are cross-reactive with native CCR5 upon immunization.
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Abstract
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Priority Applications (6)
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---|---|---|---|
AU2007277556A AU2007277556B2 (en) | 2006-07-26 | 2007-07-26 | Immunogenic compounds and protein mimics |
EP07793875A EP2052253A2 (en) | 2006-07-26 | 2007-07-26 | Immunogenic compounds and protein mimics |
NZ574423A NZ574423A (en) | 2006-07-26 | 2007-07-26 | Immunogenic compounds and protein mimics |
JP2009521718A JP2009544697A (en) | 2006-07-26 | 2007-07-26 | Immunogenic compounds and protein mimetics |
CA002659225A CA2659225A1 (en) | 2006-07-26 | 2007-07-26 | Immunogenic compounds and protein mimics |
US12/309,751 US20100322945A1 (en) | 2006-07-26 | 2007-07-26 | Immunogenic compounds and protein mimics |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
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EP06076478.4 | 2006-07-26 | ||
EP06076478 | 2006-07-26 |
Publications (3)
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WO2008013454A2 true WO2008013454A2 (en) | 2008-01-31 |
WO2008013454A3 WO2008013454A3 (en) | 2008-04-03 |
WO2008013454B1 WO2008013454B1 (en) | 2008-06-05 |
Family
ID=37564322
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PCT/NL2007/050374 WO2008013454A2 (en) | 2006-07-26 | 2007-07-26 | Immunogenic compounds and protein mimics |
Country Status (7)
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US (1) | US20100322945A1 (en) |
EP (1) | EP2052253A2 (en) |
JP (1) | JP2009544697A (en) |
AU (1) | AU2007277556B2 (en) |
CA (1) | CA2659225A1 (en) |
NZ (1) | NZ574423A (en) |
WO (1) | WO2008013454A2 (en) |
Cited By (27)
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JP2011514803A (en) * | 2008-02-05 | 2011-05-12 | メディカル リサーチ カウンシル | Methods and compositions |
WO2012057624A1 (en) | 2010-10-25 | 2012-05-03 | Pepscan Systems B.V. | Novel bicyclic peptide mimetics |
WO2012066058A1 (en) | 2010-11-16 | 2012-05-24 | Boehringer Ingelheim International Gmbh | Agents and methods for treating diseases that correlate with bcma expression |
WO2014136013A1 (en) | 2013-03-04 | 2014-09-12 | Koninklijke Philips N.V. | A microdermabrasion device |
WO2015011467A1 (en) | 2013-07-26 | 2015-01-29 | Isis Innovation Limited | Identification and display of peptide ligands |
US9175056B2 (en) | 2006-12-14 | 2015-11-03 | Alleron Therapeutics, Inc. | Bis-sulfhydryl macrocyclization systems |
US9522947B2 (en) | 2011-10-18 | 2016-12-20 | Aileron Therapeutics, Inc. | Peptidomimetic macrocycles |
US9827291B2 (en) | 2013-01-30 | 2017-11-28 | Ngm Biopharmaceuticals, Inc. | Compositions and methods of use for treating metabolic disorders |
US9828415B2 (en) | 2013-01-30 | 2017-11-28 | Ngm Biopharmaceuticals, Inc. | Compositions and methods of use for treating metabolic disorders |
US9834586B2 (en) | 2014-07-30 | 2017-12-05 | Ngm Biopharmaceuticals, Inc. | Compositions and methods of use for treating metabolic disorders |
US9845287B2 (en) | 2012-11-01 | 2017-12-19 | Aileron Therapeutics, Inc. | Disubstituted amino acids and methods of preparation and use thereof |
US9920118B2 (en) | 2014-10-31 | 2018-03-20 | Ngm Biopharmaceuticals, Inc. | Compositions and methods of use for treating metabolic disorders |
US9957299B2 (en) | 2010-08-13 | 2018-05-01 | Aileron Therapeutics, Inc. | Peptidomimetic macrocycles |
US10023613B2 (en) | 2015-09-10 | 2018-07-17 | Aileron Therapeutics, Inc. | Peptidomimetic macrocycles as modulators of MCL-1 |
US10022422B2 (en) | 2009-01-14 | 2018-07-17 | Alleron Therapeutics, Inc. | Peptidomimetic macrocycles |
US10059741B2 (en) | 2015-07-01 | 2018-08-28 | Aileron Therapeutics, Inc. | Peptidomimetic macrocycles |
US10174119B2 (en) | 2016-03-31 | 2019-01-08 | Ngm Biopharmaceuticals, Inc. | Binding proteins and methods of use thereof |
US10195250B2 (en) | 2012-03-27 | 2019-02-05 | Ngm Biopharmaceuticals, Inc. | Compositions and methods of use for treating metabolic disorders |
US10213477B2 (en) | 2012-02-15 | 2019-02-26 | Aileron Therapeutics, Inc. | Peptidomimetic macrocycles |
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US10227380B2 (en) | 2012-02-15 | 2019-03-12 | Aileron Therapeutics, Inc. | Triazole-crosslinked and thioether-crosslinked peptidomimetic macrocycles |
US10253067B2 (en) | 2015-03-20 | 2019-04-09 | Aileron Therapeutics, Inc. | Peptidomimetic macrocycles and uses thereof |
US10301351B2 (en) | 2007-03-28 | 2019-05-28 | President And Fellows Of Harvard College | Stitched polypeptides |
US10471120B2 (en) | 2014-09-24 | 2019-11-12 | Aileron Therapeutics, Inc. | Peptidomimetic macrocycles and uses thereof |
WO2020169987A1 (en) | 2019-02-22 | 2020-08-27 | Mologic Limited | Treatment stratification for an exacerbation of inflammation |
US10905739B2 (en) | 2014-09-24 | 2021-02-02 | Aileron Therapeutics, Inc. | Peptidomimetic macrocycles and formulations thereof |
AU2017353336B2 (en) * | 2016-11-01 | 2022-06-30 | Matn Scientific Limited | Detection and treatment of demyelinating diseases |
Families Citing this family (4)
Publication number | Priority date | Publication date | Assignee | Title |
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EP1125905A1 (en) * | 2000-02-16 | 2001-08-22 | Pepscan Systems B.V. | Segment synthesis |
EP1452868A2 (en) | 2003-02-27 | 2004-09-01 | Pepscan Systems B.V. | Method for selecting a candidate drug compound |
ES2545614T3 (en) | 2009-02-06 | 2015-09-14 | Pepscan Systems Bv | Truncated proteins with cystine knot |
EP2764140B1 (en) * | 2011-10-07 | 2017-08-30 | Bicycle Therapeutics Limited | Modulation of structured polypeptide specificity |
Family Cites Families (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CA2047042A1 (en) * | 1990-07-19 | 1992-01-20 | John Hannah | Cyclic hiv principal neutralizing determinant peptides |
GB0020717D0 (en) * | 2000-08-22 | 2000-10-11 | Smithkline Beecham Biolog | Novel compounds and process |
FR2839452A1 (en) * | 2002-05-07 | 2003-11-14 | Pf Medicament | USE OF PEPTIDE DERIVED FROM THE HCG BETA SUBUNIT TO GENERATE A CTL ANTITUMOR RESPONSE |
GB0213878D0 (en) * | 2002-06-17 | 2002-07-31 | Protherics Plc | Use |
EP1452868A2 (en) * | 2003-02-27 | 2004-09-01 | Pepscan Systems B.V. | Method for selecting a candidate drug compound |
SI1844337T1 (en) * | 2005-01-24 | 2013-11-29 | Pepscan Systems B.V. | Binding compounds, immunogenic compounds and peptidomimetics |
-
2007
- 2007-07-26 US US12/309,751 patent/US20100322945A1/en not_active Abandoned
- 2007-07-26 EP EP07793875A patent/EP2052253A2/en not_active Withdrawn
- 2007-07-26 WO PCT/NL2007/050374 patent/WO2008013454A2/en active Application Filing
- 2007-07-26 CA CA002659225A patent/CA2659225A1/en not_active Abandoned
- 2007-07-26 NZ NZ574423A patent/NZ574423A/en not_active IP Right Cessation
- 2007-07-26 AU AU2007277556A patent/AU2007277556B2/en not_active Ceased
- 2007-07-26 JP JP2009521718A patent/JP2009544697A/en active Pending
Non-Patent Citations (1)
Title |
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None |
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Also Published As
Publication number | Publication date |
---|---|
NZ574423A (en) | 2012-04-27 |
EP2052253A2 (en) | 2009-04-29 |
CA2659225A1 (en) | 2008-01-31 |
AU2007277556A1 (en) | 2008-01-31 |
AU2007277556B2 (en) | 2012-11-29 |
US20100322945A1 (en) | 2010-12-23 |
JP2009544697A (en) | 2009-12-17 |
WO2008013454A3 (en) | 2008-04-03 |
WO2008013454B1 (en) | 2008-06-05 |
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