WO2008011070A3 - A method of oocyte cryopreservation including piercing the zona pellucida prior to vitrification - Google Patents

A method of oocyte cryopreservation including piercing the zona pellucida prior to vitrification Download PDF

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Publication number
WO2008011070A3
WO2008011070A3 PCT/US2007/016308 US2007016308W WO2008011070A3 WO 2008011070 A3 WO2008011070 A3 WO 2008011070A3 US 2007016308 W US2007016308 W US 2007016308W WO 2008011070 A3 WO2008011070 A3 WO 2008011070A3
Authority
WO
WIPO (PCT)
Prior art keywords
oocyte
vitrification
zona pellucida
including piercing
oocyte cryopreservation
Prior art date
Application number
PCT/US2007/016308
Other languages
French (fr)
Other versions
WO2008011070A2 (en
Inventor
Geoffery Sher
Levent Keskintepe
Tetsunori Mukaida
Original Assignee
Reprocure Llc
Geoffery Sher
Levent Keskintepe
Tetsunori Mukaida
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Reprocure Llc, Geoffery Sher, Levent Keskintepe, Tetsunori Mukaida filed Critical Reprocure Llc
Publication of WO2008011070A2 publication Critical patent/WO2008011070A2/en
Publication of WO2008011070A3 publication Critical patent/WO2008011070A3/en

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Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02—Preservation of living parts
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02—Preservation of living parts
    • A01N1/0205—Chemical aspects
    • A01N1/021—Preservation or perfusion media, liquids, solids or gases used in the preservation of cells, tissue, organs or bodily fluids
    • A01N1/0221—Freeze-process protecting agents, i.e. substances protecting cells from effects of the physical process, e.g. cryoprotectants, osmolarity regulators like oncotic agents

Abstract

The present invention relates to the cryopreservation and preparation of warmed oocytes for fertilization. Cryopreservation of oocytes using vitrification methods is disclosed. Exemplary techniques include making a vent in the zona pellucida surrounding the oocyte; contacting the oocyte with a vitrification solution comprising at least one cryoprotectant; and vitrifying the oocyte. Further methods of warming the vitrified oocyte, in preparation for intracellular sperm injection are disclosed, allowing for the assessment of successful fertilization.
PCT/US2007/016308 2006-07-19 2007-07-19 A method of oocyte cryopreservation including piercing the zona pellucida prior to vitrification WO2008011070A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US83168706P 2006-07-19 2006-07-19
US60/831,687 2006-07-19

Publications (2)

Publication Number Publication Date
WO2008011070A2 WO2008011070A2 (en) 2008-01-24
WO2008011070A3 true WO2008011070A3 (en) 2008-03-20

Family

ID=38860334

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2007/016308 WO2008011070A2 (en) 2006-07-19 2007-07-19 A method of oocyte cryopreservation including piercing the zona pellucida prior to vitrification

Country Status (1)

Country Link
WO (1) WO2008011070A2 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102009022580A1 (en) * 2009-05-25 2010-12-02 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Method and device for preserving cell nuclei
JP6012164B2 (en) * 2011-11-25 2016-10-25 住友化学株式会社 Method for cryopreserving tissue derived from pluripotent stem cells
US11617365B2 (en) 2015-10-13 2023-04-04 Fertilesafe Ltd. Devices and methods for preparation of a biological sample for a cryoprocedure
WO2018069756A1 (en) * 2016-10-13 2018-04-19 Fertilesafe Ltd. Devices and methods for warming of a cryopreserved biological sample
CN110367241A (en) * 2019-07-26 2019-10-25 佛山科学技术学院 A kind of carp spermatozoa preservative fluid and its store method and Activiation method

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001000795A1 (en) * 1999-06-30 2001-01-04 Hwang, Woo-Suk A method for producing cloned cows
WO2001068815A1 (en) * 2000-03-14 2001-09-20 Es Cell International Pte Ltd Embryonic stem cells and neural progenitor cells derived therefrom
WO2001093676A1 (en) * 2000-06-16 2001-12-13 The United States Of America, As Represented By The Secretary Of Agriculture Cryopreservation of swine embryos
WO2004098285A2 (en) * 2003-05-08 2004-11-18 Cellartis Ab Cryopreservation of human blastocyst-derived stem cells by use of a closed straw vitrification method

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001000795A1 (en) * 1999-06-30 2001-01-04 Hwang, Woo-Suk A method for producing cloned cows
WO2001068815A1 (en) * 2000-03-14 2001-09-20 Es Cell International Pte Ltd Embryonic stem cells and neural progenitor cells derived therefrom
WO2001093676A1 (en) * 2000-06-16 2001-12-13 The United States Of America, As Represented By The Secretary Of Agriculture Cryopreservation of swine embryos
WO2004098285A2 (en) * 2003-05-08 2004-11-18 Cellartis Ab Cryopreservation of human blastocyst-derived stem cells by use of a closed straw vitrification method

Also Published As

Publication number Publication date
WO2008011070A2 (en) 2008-01-24

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