WO2008005246A3 - Systems and methods for monitoring the amplification and dissociation behavior of dna molecules - Google Patents
Systems and methods for monitoring the amplification and dissociation behavior of dna molecules Download PDFInfo
- Publication number
- WO2008005246A3 WO2008005246A3 PCT/US2007/014860 US2007014860W WO2008005246A3 WO 2008005246 A3 WO2008005246 A3 WO 2008005246A3 US 2007014860 W US2007014860 W US 2007014860W WO 2008005246 A3 WO2008005246 A3 WO 2008005246A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- view
- field
- dna molecules
- amplification
- monitoring
- Prior art date
Links
Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502769—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements
- B01L3/502784—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for droplet or plug flow, e.g. digital microfluidics
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
- B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
- B01L7/525—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples with physical movement of samples between temperature zones
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/6452—Individual samples arranged in a regular 2D-array, e.g. multiwell plates
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/06—Fluid handling related problems
- B01L2200/0673—Handling of plugs of fluid surrounded by immiscible fluid
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/14—Process control and prevention of errors
- B01L2200/143—Quality control, feedback systems
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0809—Geometry, shape and general structure rectangular shaped
- B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/18—Means for temperature control
- B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
- B01L2300/1822—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks using Peltier elements
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5025—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures for parallel transport of multiple samples
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Dispersion Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Analytical Chemistry (AREA)
- Clinical Laboratory Science (AREA)
- Hematology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The present invention relates to systems and methods for monitoring the amplification of DNA molecules and the dissociation behavior of the DNA molecules. The present invention in one embodiment provides a system that includes a microfluidic channel comprising a PCR processing zone and an HRTm analysis zone; and an image sensor having a first image sensor region having a first field of view and a second image sensor region having a second field of view, wherein the second field of view is different than the first field of view, wherein at least a portion of the PCR processing zone is within the first field of view; and at least a portion of the HRTm analysis zone is within the second field of view.
Priority Applications (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2009518239A JP2009542209A (en) | 2006-06-30 | 2007-06-27 | System and method for monitoring the amplification and dissociation behavior of DNA molecules |
EP07796480A EP2041260A2 (en) | 2006-06-30 | 2007-06-27 | Systems and methods for monitoring the amplification and dissociation behavior of dna molecules |
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US80644006P | 2006-06-30 | 2006-06-30 | |
US60/806,440 | 2006-06-30 | ||
US11/606,204 | 2006-11-30 | ||
US11/606,204 US7906319B2 (en) | 2006-06-30 | 2006-11-30 | Systems and methods for monitoring the amplification and dissociation behavior of DNA molecules |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2008005246A2 WO2008005246A2 (en) | 2008-01-10 |
WO2008005246A3 true WO2008005246A3 (en) | 2008-12-04 |
Family
ID=38877104
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US2007/014860 WO2008005246A2 (en) | 2006-06-30 | 2007-06-27 | Systems and methods for monitoring the amplification and dissociation behavior of dna molecules |
Country Status (1)
Country | Link |
---|---|
WO (1) | WO2008005246A2 (en) |
Families Citing this family (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US9393566B2 (en) | 2008-06-23 | 2016-07-19 | Canon U.S. Life Sciences, Inc. | System and method for temperature referencing for melt curve data collection |
CN101724295B (en) * | 2009-12-03 | 2013-03-13 | 中国农业大学 | LE Green mixed dye and applications thereof |
JP5574855B2 (en) * | 2010-07-02 | 2014-08-20 | 三菱電機株式会社 | Wireless communication system, wireless base station, wireless terminal station, and wireless communication method |
EP2878374B1 (en) | 2013-11-29 | 2021-05-12 | IMEC vzw | Method for performing digital PCR |
Citations (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5498392A (en) * | 1992-05-01 | 1996-03-12 | Trustees Of The University Of Pennsylvania | Mesoscale polynucleotide amplification device and method |
US20020197603A1 (en) * | 1997-09-02 | 2002-12-26 | Calvin Y.H. Chow | Electrical current for controlling fluid parameters in microchannels |
US6551841B1 (en) * | 1992-05-01 | 2003-04-22 | The Trustees Of The University Of Pennsylvania | Device and method for the detection of an analyte utilizing mesoscale flow systems |
US20030104466A1 (en) * | 1997-04-04 | 2003-06-05 | Caliper Technologies Corporation | Microfluidic sequencing systems |
US20050158725A1 (en) * | 2002-09-24 | 2005-07-21 | Tetsuo Yukimasa | Method of amplifying nucleic acid by electromagnetic induction heating and reaction container and reaction device to be used therein |
US20050202470A1 (en) * | 2000-11-16 | 2005-09-15 | Caliper Life Sciences, Inc. | Binding assays using molecular melt curves |
US20050221373A1 (en) * | 2001-04-06 | 2005-10-06 | California Institute Of Technology | Nucleic acid amplification using microfluidic devices |
US20050244933A1 (en) * | 2004-04-28 | 2005-11-03 | International Business Machines Corporation | Method and apparatus for precise temperature cycling in chemical/biochemical processes |
-
2007
- 2007-06-27 WO PCT/US2007/014860 patent/WO2008005246A2/en active Application Filing
Patent Citations (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5498392A (en) * | 1992-05-01 | 1996-03-12 | Trustees Of The University Of Pennsylvania | Mesoscale polynucleotide amplification device and method |
US6551841B1 (en) * | 1992-05-01 | 2003-04-22 | The Trustees Of The University Of Pennsylvania | Device and method for the detection of an analyte utilizing mesoscale flow systems |
US20030104466A1 (en) * | 1997-04-04 | 2003-06-05 | Caliper Technologies Corporation | Microfluidic sequencing systems |
US20020197603A1 (en) * | 1997-09-02 | 2002-12-26 | Calvin Y.H. Chow | Electrical current for controlling fluid parameters in microchannels |
US20050202470A1 (en) * | 2000-11-16 | 2005-09-15 | Caliper Life Sciences, Inc. | Binding assays using molecular melt curves |
US20050221373A1 (en) * | 2001-04-06 | 2005-10-06 | California Institute Of Technology | Nucleic acid amplification using microfluidic devices |
US20050158725A1 (en) * | 2002-09-24 | 2005-07-21 | Tetsuo Yukimasa | Method of amplifying nucleic acid by electromagnetic induction heating and reaction container and reaction device to be used therein |
US20050244933A1 (en) * | 2004-04-28 | 2005-11-03 | International Business Machines Corporation | Method and apparatus for precise temperature cycling in chemical/biochemical processes |
Also Published As
Publication number | Publication date |
---|---|
WO2008005246A2 (en) | 2008-01-10 |
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