WO2008000511A3 - Pektin methyltransferases and their applications - Google Patents

Pektin methyltransferases and their applications Download PDF

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Publication number
WO2008000511A3
WO2008000511A3 PCT/EP2007/005794 EP2007005794W WO2008000511A3 WO 2008000511 A3 WO2008000511 A3 WO 2008000511A3 EP 2007005794 W EP2007005794 W EP 2007005794W WO 2008000511 A3 WO2008000511 A3 WO 2008000511A3
Authority
WO
WIPO (PCT)
Prior art keywords
methyltransferases
pektin
applications
methods
producing
Prior art date
Application number
PCT/EP2007/005794
Other languages
French (fr)
Other versions
WO2008000511A2 (en
Inventor
Thomas Schmuelling
Eva Krupkova
Original Assignee
Univ Berlin Freie
Thomas Schmuelling
Eva Krupkova
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Berlin Freie, Thomas Schmuelling, Eva Krupkova filed Critical Univ Berlin Freie
Publication of WO2008000511A2 publication Critical patent/WO2008000511A2/en
Publication of WO2008000511A3 publication Critical patent/WO2008000511A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8245—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
    • C12N15/8246—Non-starch polysaccharides, e.g. cellulose, fructans, levans
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1003—Transferases (2.) transferring one-carbon groups (2.1)
    • C12N9/1007—Methyltransferases (general) (2.1.1.)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Nutrition Science (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

The invention relates to transgenic plants with reduced or enhanced pectin methyltransferases activity, methods for producing such plants and to their use as well as methods of producing modified pections and to compositions comprising such modified pectins.
PCT/EP2007/005794 2006-06-30 2007-06-29 Pektin methyltransferases and their applications WO2008000511A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US81774206P 2006-06-30 2006-06-30
US60/817,742 2006-06-30

Publications (2)

Publication Number Publication Date
WO2008000511A2 WO2008000511A2 (en) 2008-01-03
WO2008000511A3 true WO2008000511A3 (en) 2008-02-21

Family

ID=38512474

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2007/005794 WO2008000511A2 (en) 2006-06-30 2007-06-29 Pektin methyltransferases and their applications

Country Status (1)

Country Link
WO (1) WO2008000511A2 (en)

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997010328A2 (en) * 1995-07-13 1997-03-20 Ribozyme Pharmaceuticals, Inc. Compositions and method for modulation of gene expression in plants
WO2001059137A1 (en) * 2000-02-10 2001-08-16 Biologic A/S Method for remodelling cell wall polysaccharide structures in plants
US20030014775A1 (en) * 1994-09-02 2003-01-16 Ribozyme Pharmaceuticals, Inc. Compositions and method for modulation of gene expression in plants
US20030093837A1 (en) * 1999-03-23 2003-05-15 James Keddie Polynucleotides for seed trait alteration
US20030101481A1 (en) * 1998-09-22 2003-05-29 James Zhang Plant gene sequences I
US20040045049A1 (en) * 1998-09-22 2004-03-04 James Zhang Polynucleotides and polypeptides in plants

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030014775A1 (en) * 1994-09-02 2003-01-16 Ribozyme Pharmaceuticals, Inc. Compositions and method for modulation of gene expression in plants
WO1997010328A2 (en) * 1995-07-13 1997-03-20 Ribozyme Pharmaceuticals, Inc. Compositions and method for modulation of gene expression in plants
US20030101481A1 (en) * 1998-09-22 2003-05-29 James Zhang Plant gene sequences I
US20040045049A1 (en) * 1998-09-22 2004-03-04 James Zhang Polynucleotides and polypeptides in plants
US20030093837A1 (en) * 1999-03-23 2003-05-15 James Keddie Polynucleotides for seed trait alteration
WO2001059137A1 (en) * 2000-02-10 2001-08-16 Biologic A/S Method for remodelling cell wall polysaccharide structures in plants

Non-Patent Citations (10)

* Cited by examiner, † Cited by third party
Title
BOURLARD T ET AL: "Purification of several pectin methyltransferases from cell suspension cultures of flax (Linum usitatissimum L.)", COMPTES RENDUS DES SEANCES DE L'ACADEMIE DES SCIENCES. SERIE III: SCIENCES DE LA VIE, ELSEVIER, AMSTERDAM, NL, vol. 324, no. 4, April 2001 (2001-04-01), pages 335 - 343, XP004330734, ISSN: 0764-4469 *
BOUTON SOPHIE ET AL: "Quasimodo1 encodes a putative membrane-bound glycosyltransferase required for normal pectin synthesis and cell adhesion in Arabidopsis", PLANT CELL, vol. 14, no. 10, October 2002 (2002-10-01), pages 2577 - 2590, XP002452281, ISSN: 1040-4651 *
BRUMMELL D A ET AL: "Cell wall metabolism in fruit softening and quality and its manipulation in transgenic plants", PLANT MOLECULAR BIOLOGY, SPRINGER, DORDRECHT, NL, vol. 47, no. 1-2, September 2001 (2001-09-01), pages 311 - 340, XP002366736, ISSN: 0167-4412 *
DATABASE REFSEQ [online] NCBI; 9 June 2006 (2006-06-09), ANONYMOUS: "Arabidopsis thaliana unknown protein (AT1G78240) mRNA, complete cds.", XP002452306, Database accession no. NM_106474 *
DATABASE REFSEQ [online] NCBI; 9 June 2006 (2006-06-09), ANONYMOUS: "unknown protein [Arabidopsis thaliana]", XP002452305, Database accession no. NP_177948 *
FRANK MARKUS ET AL: "Tumorous shoot development (TSD) genes are required for co-ordinated plant shoot development", PLANT JOURNAL, vol. 29, no. 1, January 2002 (2002-01-01), pages 73 - 85, XP002452220, ISSN: 0960-7412 *
IWAI H ET AL: "A pectin glucuronyltransferase gene is essential for intercellular attachment in the plant meristem", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, NATIONAL ACADEMY OF SCIENCE, WASHINGTON, DC, US, vol. 99, no. 25, 10 December 2002 (2002-12-10), pages 16319 - 16324, XP002904270, ISSN: 0027-8424 *
KRUPKOVA EVA ET AL: "The TUMOROUS SHOOT DEVELOPMENT2 gene of Arabidopsis encoding a putative methyltransferase is required for cell adhesion and co-ordinated plant development", PLANT JOURNAL, vol. 50, no. 4, May 2007 (2007-05-01), pages 735 - 750, XP002452219, ISSN: 0960-7412 *
KRUPKOVÁ, EVA: "Characterisation of tumorous shoot development (tsd) mutants of Arabidopsis thaliana and identification of the TSD1 and TSD2 genes", THESIS, 2004, Freie Universität Berlin, Berlin, pages 1 - 134, XP008085905 *
VOGEL JOHN P ET AL: "Mutations in PMR5 result in powdery mildew resistance and altered cell wall composition", PLANT JOURNAL, vol. 40, no. 6, December 2004 (2004-12-01), pages 968 - 978, XP002452284, ISSN: 0960-7412 *

Also Published As

Publication number Publication date
WO2008000511A2 (en) 2008-01-03

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