WO2007147064A3 - Modified soy proteins and methods of use - Google Patents

Modified soy proteins and methods of use Download PDF

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Publication number
WO2007147064A3
WO2007147064A3 PCT/US2007/071235 US2007071235W WO2007147064A3 WO 2007147064 A3 WO2007147064 A3 WO 2007147064A3 US 2007071235 W US2007071235 W US 2007071235W WO 2007147064 A3 WO2007147064 A3 WO 2007147064A3
Authority
WO
WIPO (PCT)
Prior art keywords
calcium
proteins
modified
binding
protein
Prior art date
Application number
PCT/US2007/071235
Other languages
French (fr)
Other versions
WO2007147064A2 (en
Inventor
Jian G Dong
Shirong Zhang
A Gururaj Rao
Original Assignee
Pioneer Hi Bred Int
Jian G Dong
Shirong Zhang
A Gururaj Rao
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pioneer Hi Bred Int, Jian G Dong, Shirong Zhang, A Gururaj Rao filed Critical Pioneer Hi Bred Int
Publication of WO2007147064A2 publication Critical patent/WO2007147064A2/en
Publication of WO2007147064A3 publication Critical patent/WO2007147064A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
    • A23J3/00—Working-up of proteins for foodstuffs
    • A23J3/14—Vegetable proteins
    • A23J3/16—Vegetable proteins from soybean
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/16—Inorganic salts, minerals or trace elements
    • A23L33/165—Complexes or chelates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8251—Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Nutrition Science (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Food Science & Technology (AREA)
  • Plant Pathology (AREA)
  • Polymers & Plastics (AREA)
  • Botany (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Inorganic Chemistry (AREA)
  • Mycology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)

Abstract

Compositions and methods for preparing modified proteins are provided. In one embodiment, calcium-modified proteins are provided by operably linking a calcium-binding extension region capable of binding calcium to a protein of interest. The link of the extension can additionally cause increased solubility of the resulting protein and facilitate its formation of translucent gels. In this manner calcium enrich proteins can be obtained for use as food and beverage additives. Nucleotide and amino acid sequences for calcium-binding extension regions are provided and can be used to modify proteins, including soy proteins to provide calcium fortified proteins. Commercial varieties of soybeans modified to express particular soy protein, particular β-conglycinin subunits are provided. The soy proteins can be modified to contain at least one calcium-binding extension region.
PCT/US2007/071235 2006-06-14 2007-06-14 Modified soy proteins and methods of use WO2007147064A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US81353206P 2006-06-14 2006-06-14
US60/813,532 2006-06-14

Publications (2)

Publication Number Publication Date
WO2007147064A2 WO2007147064A2 (en) 2007-12-21
WO2007147064A3 true WO2007147064A3 (en) 2008-05-15

Family

ID=38832864

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2007/071235 WO2007147064A2 (en) 2006-06-14 2007-06-14 Modified soy proteins and methods of use

Country Status (1)

Country Link
WO (1) WO2007147064A2 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK2264055T3 (en) * 2009-06-17 2014-04-07 Indena Spa Cloning, yeast expression, purification and biological activity of the soybean 7S globulin alpha subunit elongation region involved in Hep-G2 cell cholesterol homeostasis
EP2264054A1 (en) * 2009-06-17 2010-12-22 Indena S.p.A. Cloning, yeast expression, purification and biological activity of a truncated form of the soybean 7S globulin alfa ' subunit involved in Hep G2 cell cholesterol homeostasis
UY37482A (en) * 2016-11-16 2018-05-31 Cellectis METHODS TO CHANGE THE CONTENT OF AMINO ACIDS IN PLANTS THROUGH FRAMEWORK DISPLACEMENT MUTATIONS
WO2019111043A1 (en) * 2017-12-08 2019-06-13 Industrias Nutrigrains, S.A.P.I. De C.V. Protein compositions of vegetable-origin and method for obtaining the same

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
DATABASE UniProt [online] 1 December 2001 (2001-12-01), "Beta-conglycinin alpha subunit.", XP002472519, retrieved from EBI accession no. UNIPROT:Q94LX2 Database accession no. Q94LX2 *
DATABASE UniProt [online] 1 March 2001 (2001-03-01), "Alpha' subunit of beta-conglycinin (Fragment).", XP002472520, retrieved from EBI accession no. UNIPROT:Q9FZP9 Database accession no. Q9FZP9 *
MARUYAMA N ET AL: "The roles of the N-linked glycans and extension regions of soybean beta-conglycinin in folding, assembly and structural features", EUROPEAN JOURNAL OF BIOCHEMISTRY, BERLIN, DE, vol. 258, 1998, pages 854 - 862, XP003004727, ISSN: 0014-2956 *
RAO A G A ET AL: "BINDING OF CALCIUM MAGNESIUM II AND ZINC II BY 7S FRACTION OF SOYBEAN PROTEINS", JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, vol. 24, no. 3, 1976, pages 490 - 494, XP001538100, ISSN: 0021-8561 *
TANDANG MARY ROSE G ET AL: "Evaluation of the solubility and emulsifying property of soybean proglycinin and rapeseed procruciferin in relation to structure modified by protein engineering", JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, vol. 53, no. 22, November 2005 (2005-11-01), pages 8736 - 8744, XP009097163, ISSN: 0021-8561 *

Also Published As

Publication number Publication date
WO2007147064A2 (en) 2007-12-21

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