WO2007143436A3 - Nouvelle adn polymérase issue de spirochaeta thermophila - Google Patents

Nouvelle adn polymérase issue de spirochaeta thermophila Download PDF

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Publication number
WO2007143436A3
WO2007143436A3 PCT/US2007/069837 US2007069837W WO2007143436A3 WO 2007143436 A3 WO2007143436 A3 WO 2007143436A3 US 2007069837 W US2007069837 W US 2007069837W WO 2007143436 A3 WO2007143436 A3 WO 2007143436A3
Authority
WO
WIPO (PCT)
Prior art keywords
dna
spirochaeta thermophila
dna polymerase
polymerase
spirochaeta
Prior art date
Application number
PCT/US2007/069837
Other languages
English (en)
Other versions
WO2007143436A2 (fr
Inventor
Guolu Hu
Elena Garnova
Haiguang Xiao
Original Assignee
Ge Healthcare Bio Sciences
Guolu Hu
Elena Garnova
Haiguang Xiao
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ge Healthcare Bio Sciences, Guolu Hu, Elena Garnova, Haiguang Xiao filed Critical Ge Healthcare Bio Sciences
Publication of WO2007143436A2 publication Critical patent/WO2007143436A2/fr
Publication of WO2007143436A3 publication Critical patent/WO2007143436A3/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

L'invention concerne une ADN polymérase issue de Spirochaeta thermophila. Cette enzyme se révèle utile pour des procédés qui requièrent une synthèse d'ADN impliquant le déplacement de brins, telle que l'amplification par déplacement de brin (SDA), pour un séquençage de l'ADN, et la réaction en chaîne par polymérase. La présente invention inclut également dans son champ de réalisation divers mutants (délétion et substitution) qui conservent la capacité de répliquer l'ADN sous la forme de la polymérase native de Spirochaeta thermophila.
PCT/US2007/069837 2006-05-30 2007-05-29 Nouvelle adn polymérase issue de spirochaeta thermophila WO2007143436A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US80342406P 2006-05-30 2006-05-30
US60/803,424 2006-05-30

Publications (2)

Publication Number Publication Date
WO2007143436A2 WO2007143436A2 (fr) 2007-12-13
WO2007143436A3 true WO2007143436A3 (fr) 2008-01-31

Family

ID=38654588

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2007/069837 WO2007143436A2 (fr) 2006-05-30 2007-05-29 Nouvelle adn polymérase issue de spirochaeta thermophila

Country Status (1)

Country Link
WO (1) WO2007143436A2 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP7324200B2 (ja) 2018-07-13 2023-08-09 タカラバイオ株式会社 Ｒｎａからの核酸増幅反応に適したｄｎａポリメラーゼ変異体

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104726418A (zh) * 2015-02-10 2015-06-24 江南大学 Spirochaeta thermophila生产耐热超氧化物歧化酶(SOD)的方法

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
WO2003025132A2 (fr) * 2001-09-14 2003-03-27 Invitrogen Corporation Adn polymerases et mutants correspondants

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683195B1 (fr) * 1986-01-30 1990-11-27 Cetus Corp
WO2003025132A2 (fr) * 2001-09-14 2003-03-27 Invitrogen Corporation Adn polymerases et mutants correspondants

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
DATABASE EMBL [online] 25 December 2003 (2003-12-25), "Spirochaeta thermophila strain Z-1203 DNA polymerase I (polA) gene, complete cds.", XP002458203, retrieved from EBI accession no. EMBL:AY247648 Database accession no. AY247648 *
HUIMIN KONG ET AL: "CHARACTERIZATION OF A DNA POLYMERASE FROM THE HYPERTHERMOPHILE ARCHAEA THERMOCOCCUS LITORALIS VENT DNA POLYMERASE, STEADY STATE KINETICS, THERMAL STABILITY, PROCESSIVITY, STRAND DISPLACEMENT, AND EXONUCLEASE ACTIVITIES", JOURNAL OF BIOLOGICAL CHEMISTRY, AMERICAN SOCIETY OF BIOLOCHEMICAL BIOLOGISTS, BIRMINGHAM,, US, vol. 268, no. 3, 25 January 1993 (1993-01-25), pages 1965 - 1975, XP000611227, ISSN: 0021-9258 *
SHANDILYA H ET AL: "Thermophilic bacterial DNA polymerases with reverse-transcriptase activity", EXTREMOPHILES, SPRINGER VERLAG, TOKYO,, JP, vol. 8, no. 3, June 2004 (2004-06-01), pages 243 - 251, XP002340627, ISSN: 1431-0651 *
TERRANCE WALKER G: "ISOTHERMAL IN VITRO AMPLIFICATION OF DNA BY A RESTRICTION ENZYME/ DNA POLYMERASE SYSTEM", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, NATIONAL ACADEMY OF SCIENCE, WASHINGTON, DC, US, vol. 89, no. 1, January 1992 (1992-01-01), pages 392 - 396, XP000368694, ISSN: 0027-8424 *
VIGUERA E ET AL: "In vitro replication slippage by DNA polymerases from thermophilic organisms", JOURNAL OF MOLECULAR BIOLOGY, LONDON, GB, vol. 312, no. 2, 14 September 2001 (2001-09-14), pages 323 - 333, XP004449577, ISSN: 0022-2836 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP7324200B2 (ja) 2018-07-13 2023-08-09 タカラバイオ株式会社 Ｒｎａからの核酸増幅反応に適したｄｎａポリメラーゼ変異体

Also Published As

Publication number Publication date
WO2007143436A2 (fr) 2007-12-13

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