WO2007136693A3 - Prediction of relative polypeptide solubility by polyethylene glycol precipitation - Google Patents

Prediction of relative polypeptide solubility by polyethylene glycol precipitation Download PDF

Info

Publication number
WO2007136693A3
WO2007136693A3 PCT/US2007/011818 US2007011818W WO2007136693A3 WO 2007136693 A3 WO2007136693 A3 WO 2007136693A3 US 2007011818 W US2007011818 W US 2007011818W WO 2007136693 A3 WO2007136693 A3 WO 2007136693A3
Authority
WO
WIPO (PCT)
Prior art keywords
solubility
relative
polyethylene glycol
peg
prediction
Prior art date
Application number
PCT/US2007/011818
Other languages
French (fr)
Other versions
WO2007136693A2 (en
Inventor
Li Li
Angela Kantor
Nicholas W Warne
Original Assignee
Wyeth Corp
Li Li
Angela Kantor
Nicholas W Warne
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Wyeth Corp, Li Li, Angela Kantor, Nicholas W Warne filed Critical Wyeth Corp
Priority to AU2007254259A priority Critical patent/AU2007254259A1/en
Priority to CA002652237A priority patent/CA2652237A1/en
Priority to JP2009512051A priority patent/JP2009537846A/en
Priority to MX2008014687A priority patent/MX2008014687A/en
Priority to EP07794977A priority patent/EP2018567A2/en
Publication of WO2007136693A2 publication Critical patent/WO2007136693A2/en
Publication of WO2007136693A3 publication Critical patent/WO2007136693A3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Immunology (AREA)
  • Urology & Nephrology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Biophysics (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

A method is described for predicting the relative solubility of a polypeptide using polyethylene glycol (PEG) based volume exclusion precipitation. Different polypeptides can be tested for their solubilities relative to each other or relative to a reference. A single polypeptide can be tested for its relative solubility under different experimental conditions. The solubility determinations can be made by comparison based on graphs plotting the log solubility of the polypeptide against a range of PEG concentrations. Additionally, a method is provided for the high throughput visual or automated screening of multiple polypeptides for relative solubility differences, in a method that can omit the step of measuring the actual solubility or actual amount of precipitation of each sample at each PEG concentration.
PCT/US2007/011818 2006-05-19 2007-05-17 Prediction of relative polypeptide solubility by polyethylene glycol precipitation WO2007136693A2 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
AU2007254259A AU2007254259A1 (en) 2006-05-19 2007-05-17 Prediction of relative polypeptide solubility by polyethylene glycol precipitation
CA002652237A CA2652237A1 (en) 2006-05-19 2007-05-17 Prediction of relative polypeptide solubility by polyethylene glycol precipitation
JP2009512051A JP2009537846A (en) 2006-05-19 2007-05-17 Prediction of polypeptide specific solubility by polyethylene glycol precipitation.
MX2008014687A MX2008014687A (en) 2006-05-19 2007-05-17 Prediction of relative polypeptide solubility by polyethylene glycol precipitation.
EP07794977A EP2018567A2 (en) 2006-05-19 2007-05-17 Prediction of relative polypeptide solubility by polyethylene glycol precipitation

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US80186206P 2006-05-19 2006-05-19
US60/801,862 2006-05-19

Publications (2)

Publication Number Publication Date
WO2007136693A2 WO2007136693A2 (en) 2007-11-29
WO2007136693A3 true WO2007136693A3 (en) 2008-01-17

Family

ID=38561748

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2007/011818 WO2007136693A2 (en) 2006-05-19 2007-05-17 Prediction of relative polypeptide solubility by polyethylene glycol precipitation

Country Status (8)

Country Link
US (1) US20080003686A1 (en)
EP (1) EP2018567A2 (en)
JP (1) JP2009537846A (en)
CN (1) CN101449166A (en)
AU (1) AU2007254259A1 (en)
CA (1) CA2652237A1 (en)
MX (1) MX2008014687A (en)
WO (1) WO2007136693A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2121753A2 (en) * 2007-02-14 2009-11-25 Amgen, Inc Method of isolating antibodies by precipitation
EP3769082A4 (en) * 2018-03-20 2021-12-15 Sanofi Pasteur Limited Methods for determining adjuvanted protein concentration and percent adsorption using intrinsic fluorescence
CN111171141A (en) * 2020-01-20 2020-05-19 苏州药明康德新药开发有限公司 Application of polyethylene glycol in preclinical safety evaluation

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000059627A1 (en) * 1999-04-05 2000-10-12 Millennium Pharmaceuticals, Inc. Formulation arrays and use thereof

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000059627A1 (en) * 1999-04-05 2000-10-12 Millennium Pharmaceuticals, Inc. Formulation arrays and use thereof

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
ANTIPOVA A S ET AL: "Effect of sucrose on the thermodynamic incompatibility of different biopolymers", CARBOHYDRATE POLYMERS, APPLIED SCIENCE PUBLISHERS, LTD. BARKING, GB, vol. 28, no. 4, December 1995 (1995-12-01), pages 359 - 365, XP004034379, ISSN: 0144-8617 *
ATHA D H ET AL: "MECHANISM OF PRECIPITATION OF PROTEINS BY POLY ETHYLENE GLYCOLS ANALYSIS IN TERMS OF EXCLUDED VOLUME", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 256, no. 23, 1981, pages 12108 - 12117, XP002454548, ISSN: 0021-9258 *
HAIRE R N ET AL: "ON THE PRECIPITATION OF PROTEINS BY POLYMERS: THE HEMOGLOBIN-POLYETHYLENE GLYCOL SYSTEM", BIOPOLYMERS, NEW YORK, NY, US, vol. 23, no. 12, December 1984 (1984-12-01), pages 2761 - 2779, XP000676364, ISSN: 0006-3525 *
HASKO F ET AL: "SOLUBILITY OF PLASMA PROTEINS IN THE PRESENCE OF POLY ETHYLENE GLYCOL", BIOTECHNOLOGY AND BIOENGINEERING, vol. 24, no. 9, 1982, pages 1931 - 1939, XP002454549, ISSN: 0006-3592 *
MAHADEVAN HARI ET AL: "Experimental analysis of protein precipitation by polyethylene glycol and comparison with theory", FLUID PHASE EQUILIB; FLUID PHASE EQUILIBRIA OCT 1992, vol. 78, October 1992 (1992-10-01), pages 297 - 231, XP002454547 *
MIDDAUGH C R ET AL: "DETERMINATION OF THE APPARENT THERMODYNAMIC ACTIVITIES OF SATURATED PROTEIN SOLUTIONS", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 254, no. 2, 1979, pages 367 - 370, XP002454550, ISSN: 0021-9258 *
SHULGIN IVAN L ET AL: "Preferential hydration and solubility of proteins in aqueous solutions of polyethylene glycol", BIOPHYSICAL CHEMISTRY, vol. 120, no. 3, April 2006 (2006-04-01), pages 188 - 198, XP005357281, ISSN: 0301-4622 *

Also Published As

Publication number Publication date
EP2018567A2 (en) 2009-01-28
MX2008014687A (en) 2008-11-27
WO2007136693A2 (en) 2007-11-29
US20080003686A1 (en) 2008-01-03
CN101449166A (en) 2009-06-03
JP2009537846A (en) 2009-10-29
AU2007254259A1 (en) 2007-11-29
CA2652237A1 (en) 2007-11-29

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