WO2007135367A3 - Screen for inflammatory response molulators - Google Patents
Screen for inflammatory response molulators Download PDFInfo
- Publication number
- WO2007135367A3 WO2007135367A3 PCT/GB2007/001766 GB2007001766W WO2007135367A3 WO 2007135367 A3 WO2007135367 A3 WO 2007135367A3 GB 2007001766 W GB2007001766 W GB 2007001766W WO 2007135367 A3 WO2007135367 A3 WO 2007135367A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- inflammatory response
- granulocytes
- molulators
- screen
- zebrafish
- Prior art date
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
- G01N33/5082—Supracellular entities, e.g. tissue, organisms
- G01N33/5088—Supracellular entities, e.g. tissue, organisms of vertebrates
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K49/00—Preparations for testing in vivo
- A61K49/0004—Screening or testing of compounds for diagnosis of disorders, assessment of conditions, e.g. renal clearance, gastric emptying, testing for diabetes, allergy, rheuma, pancreas functions
- A61K49/0008—Screening agents using (non-human) animal models or transgenic animal models or chimeric hosts, e.g. Alzheimer disease animal model, transgenic model for heart failure
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Immunology (AREA)
- Urology & Nephrology (AREA)
- Cell Biology (AREA)
- Pathology (AREA)
- Toxicology (AREA)
- General Health & Medical Sciences (AREA)
- Hematology (AREA)
- Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Diabetes (AREA)
- Food Science & Technology (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- General Physics & Mathematics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Microbiology (AREA)
- Endocrinology (AREA)
- Gastroenterology & Hepatology (AREA)
- Rheumatology (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
Screening method for the identification of agents modulating an inflammatory response. The method is based on transgenic aquatic organisms (zebrafish) expressing a reporter gene such as GFP in granulocytes, which allows direct assessment of apoptosis at injury sites in vivo. Alternatively, granulocytes from a transgenic aquatic organism (zebrafish) are used, whereby the granulocytes express a caspase cleavage site, the cleavage of which is assessed by FRET.
Priority Applications (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP07732790A EP2032981A2 (en) | 2006-05-19 | 2007-05-14 | Screen for inflammatory response molulators |
US12/301,314 US20090324500A1 (en) | 2006-05-19 | 2007-05-14 | Screen for inflammatory response modulators |
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB0609946A GB0609946D0 (en) | 2006-05-19 | 2006-05-19 | Apoptosis screen |
GB0609946.9 | 2006-05-19 | ||
GB0623120.3 | 2006-11-21 | ||
GB0623120A GB0623120D0 (en) | 2006-11-21 | 2006-11-21 | Screen for inflammatory response modulators |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2007135367A2 WO2007135367A2 (en) | 2007-11-29 |
WO2007135367A3 true WO2007135367A3 (en) | 2008-03-20 |
Family
ID=38283069
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/GB2007/001766 WO2007135367A2 (en) | 2006-05-19 | 2007-05-14 | Screen for inflammatory response molulators |
Country Status (3)
Country | Link |
---|---|
US (1) | US20090324500A1 (en) |
EP (1) | EP2032981A2 (en) |
WO (1) | WO2007135367A2 (en) |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107164457B (en) * | 2017-04-26 | 2021-01-26 | 北京工商大学 | High-throughput screening method of broad-spectrum lysozyme |
CN110402893B (en) * | 2019-06-20 | 2021-01-22 | 中山大学 | Preparation and application of Nrf2 gene-deleted zebra fish mutant |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2000073437A1 (en) * | 1999-05-27 | 2000-12-07 | Merck Frosst Canada & Co. | Assays for caspase activity using green fluorescent proteins |
FR2858177A1 (en) * | 2003-07-28 | 2005-02-04 | Genethon | USE OF FREQUENT PHENOMENA, DETECTED BY MPLSM, FOR IN VIVO MONITORING OF BIOLOGICAL EVENTS |
-
2007
- 2007-05-14 US US12/301,314 patent/US20090324500A1/en not_active Abandoned
- 2007-05-14 WO PCT/GB2007/001766 patent/WO2007135367A2/en active Application Filing
- 2007-05-14 EP EP07732790A patent/EP2032981A2/en not_active Withdrawn
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2000073437A1 (en) * | 1999-05-27 | 2000-12-07 | Merck Frosst Canada & Co. | Assays for caspase activity using green fluorescent proteins |
FR2858177A1 (en) * | 2003-07-28 | 2005-02-04 | Genethon | USE OF FREQUENT PHENOMENA, DETECTED BY MPLSM, FOR IN VIVO MONITORING OF BIOLOGICAL EVENTS |
Non-Patent Citations (6)
Title |
---|
HSU KARL ET AL: "Expression of Green Fluorescent Protein (GFP) in Transgenic Zebrafish Embryos under the Control of the Zebrafish MPO Promoter", BLOOD, W.B.SAUNDERS COMPANY, ORLANDO, FL, US, vol. 100, no. 11, 16 November 2002 (2002-11-16), pages 931, XP009087507, ISSN: 0006-4971 * |
HSU KARL ET AL: "Lessons from transgenic zebrafish expressing the green fluorescent protein (GFP) in the myeloid lineage", METHODS IN CELL BIOLOGY ELSEVIER ACADEMIC PRESS INC, 525 B STREET, SUITE 1900, SAN DIEGO, CA 92101-4495 USA SERIES : METHODS IN CELL BIOLOGY (ISSN 0091-679X(PRINT)), 2004, pages 333 - 347, XP009087508, ISSN: 0-12-564172-9(H) * |
REDD MICHAEL J ET AL: "Wound healing and inflammation: embryos reveal the way to perfect repair", PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY OF LONDON B BIOLOGICAL SCIENCES, vol. 359, no. 1445, 29 May 2004 (2004-05-29), pages 777 - 784, XP002447424, ISSN: 0962-8436 * |
RENSHAW STEPHEN A ET AL: "A transgenic zebrafish model of neutrophilic inflammation.", BLOOD 15 DEC 2006, vol. 108, no. 13, 15 December 2006 (2006-12-15), pages 3976 - 3978, XP002447426, ISSN: 0006-4971 * |
RHODES JENNIFER ET AL: "A role for fbxo5 in zebrafish myelopoiesis", BLOOD, vol. 106, no. 11, Part 1, November 2005 (2005-11-01), & 47TH ANNUAL MEETING OF THE AMERICAN-SOCIETY-OF-HEMATOLOGY; ATLANTA, GA, USA; DECEMBER 10 -13, 2005, pages 1005A - 1006A, XP002447425, ISSN: 0006-4971 * |
TYAS LORRAINE ET AL: "Rapid caspase-3 activation during apoptosis revealed using fluorescence-resonance energy transfer", EMBO REPORTS, vol. 1, no. 3, September 2000 (2000-09-01), pages 266 - 270, XP002462252, ISSN: 1469-221X * |
Also Published As
Publication number | Publication date |
---|---|
US20090324500A1 (en) | 2009-12-31 |
EP2032981A2 (en) | 2009-03-11 |
WO2007135367A2 (en) | 2007-11-29 |
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