WO2007131538A1 - Composés de pyrazoline substitués par azépane ou azocane, leur préparation et leur utilisation comme médicaments - Google Patents

Composés de pyrazoline substitués par azépane ou azocane, leur préparation et leur utilisation comme médicaments Download PDF

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WO2007131538A1
WO2007131538A1 PCT/EP2006/007412 EP2006007412W WO2007131538A1 WO 2007131538 A1 WO2007131538 A1 WO 2007131538A1 EP 2006007412 W EP2006007412 W EP 2006007412W WO 2007131538 A1 WO2007131538 A1 WO 2007131538A1
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alkyl
independently
group
ocf
chf
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PCT/EP2006/007412
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Antonio Torrens Jover
Susana Yenes Minguez
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Laboratorios Del Dr. Esteve, S.A.
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Priority claimed from EP05384018A external-priority patent/EP1743888A1/fr
Priority claimed from EP06008612A external-priority patent/EP1849776A1/fr
Application filed by Laboratorios Del Dr. Esteve, S.A. filed Critical Laboratorios Del Dr. Esteve, S.A.
Publication of WO2007131538A1 publication Critical patent/WO2007131538A1/fr

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D231/00—Heterocyclic compounds containing 1,2-diazole or hydrogenated 1,2-diazole rings
    • C07D231/02—Heterocyclic compounds containing 1,2-diazole or hydrogenated 1,2-diazole rings not condensed with other rings
    • C07D231/06—Heterocyclic compounds containing 1,2-diazole or hydrogenated 1,2-diazole rings not condensed with other rings having one double bond between ring members or between a ring member and a non-ring member
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/18—Antipsychotics, i.e. neuroleptics; Drugs for mania or schizophrenia
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
    • A61P3/10—Drugs for disorders of the metabolism for glucose homeostasis for hyperglycaemia, e.g. antidiabetics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P39/00—General protective or antinoxious agents

Definitions

  • Azepane- or Azocane-substituted pyrazoline compounds their preparation and use as medicaments
  • the present invention relates to Azepane- or Azocane-substituted substituted _ pyrazoline compounds, methods for their preparation, medicaments comprising these compounds as well as their use for the preparation of a medicament for the treatment of humans and animals.
  • Cannabinoids are compounds, which are derived from the cannabis sativa plant which is commonly known as marijuana.
  • the most active chemical compound of the naturally occurring cannabinoids is tetrahydrocannabinol (THC), particularly ⁇ 9 -THC.
  • cannabinoids as well as their synthetic analogues promote their physiological effects via binding to specific G-coupled receptors, the so-called cannabinoid-receptors.
  • CBi and CB2 are involved in a variety of physiological or pathophysiological processes in humans and animals, e.g. processes related to the central nervous system, immune system, cardiovascular system, endocrinous system, respiratory system, the gastrointestinal tract or to reproduction, as described for example, in Hollister, Pharm. Rev. 38, 1986, 1-20; Reny and Singha, Prog. Drug. Res., 36, 71-114, 1991 ; Consroe and Sandyk, in Marijuana/Cannabinoids, Neurobiology and Neurophysiology, 459, Murphy L. and Barthe A. Eds., CRC Press, 1992.
  • the CBi-Receptor is involved in many different food-intake related disorders such as bulimia or obesity, including obesity associated with type Il diabetes (non-insulin-dependent diabetes) and thus, compounds suitable for regulating this receptor may be used in the prophylaxis and/or treatment of these disorders.
  • these compounds have a high affinity for cannabinoid receptors, particularly for the CBi-receptor, and that they act as modulators e.g. antagonists, inverse agonists or agonists on these receptors. They are therefore suitable for the prophylaxis and/or treatment of various disorders related to the central nervous system, the immune system, the cardiovascular system, the endocrinous system, the respiratory system, the gastrointestinal tract or reproduction in humans and/or animals, preferably humans including infants, children and grownups.
  • the present invention relates to Azepane-or Azocane substituted pyrazoline compounds of general formula I,
  • X and Y independently represent phenyl, thienyl, naphtyl or pyridyl which groups may be substituted with 1 , 2 or 3 substituents W, which can be the same or different, selected from the group _ branched or linear or branched or linear Ci -3 -alkoxy, phenyl, hydroxy, chloro, bromo, fluoro, iodo, SH, trifluoromethyl, CHF 2 , CH 2 F, OCHF 2 , trifluoromethylthio, trifluoromethoxy, methylsulfonyl, carboxyl, trifluoromethylsulfonyl, cyano, carbamoyl, sulfamoyl and acetyl; O-P, with P denominating a prodrug group consisting of aryl, C 8-2 o-alkyl, heteroaryl, C(O)-aryl, C(O)-heteroaryl I C(
  • R 8 representing a hydrogen atom or a branched or linear d- 3 -alkyl group
  • R 9 is representing
  • R 5 , R 6 and R 7 being independently from one another selected from H, F, Cl, Br, I, OH, SH, Ci -4 alkyl, Ci -4 alkoxy, CF 3 , CHF 2 , CH 2 F, OCF 3 , a keto-group, NO 2 or NH 2 ; and n being 1 or 2; or
  • R 5 , R 6 and R 7 being independently from one another selected from H, F, Cl, Br, I, OH, SH 1 C ⁇ alkyl, Ci -4 alkoxy, CF 3 , CHF 2 , CH 2 F, OCF 3 , a keto-group, NO 2 or NH 2 ; and n being 1 or 2;
  • alkyl radical or group is understood as meaning saturated and unsaturated, linear or branched hydrocarbons, which can be unsubstituted or mono- or polysubstituted.
  • saturated alkyl encompasses e.g. -CH 3 and -CH 2 -CH 3 .
  • Ci_ 2 -alkyl represents C1- or C2-alkyl
  • Ci -3 -alkyl represents C1-, C2- or C3-alkyl
  • d-4-alkyl represents C1-, C2-, C3- or C4-alkyl
  • Ci -5 -alkyl represents C1-, C2-, C3-, C4-, or C5- alkyl
  • Ci -6 -alkyl represents C1-, C2-, C3-, C4-, C5- or C6-alkyl
  • Ci -7 -alkyl represents C1-, C2-, C3-, C4-, C5-, C6- or C7-alkyl
  • C 1-8 -alkyl represents C1-, C2-, C3-, C4-, C5- , C6-, C7- or C8-alkyl
  • Ci.io-alkyl represents C1-, C2-, C3-, C4-, C5-, C
  • the alkyl radicals are preferably methyl, ethyl, vinyl (ethenyl), propyl, allyl (2-propenyl), 1-propinyl, methylethyl, butyl, 1-methylpropyl, 2-methylpropyl, 1 ,1 -dimethyl ethyl, pentyl, 1 ,1- dimethylpropyl, 1 ,2-dimethylpropyl, 2,2-dimethylpropyl, hexyl, 1 -methyl pentyl, if substituted also CHF 2 , CF 3 or CH 2 OH etc.
  • cycloalkyl radical or group is understood as meaning saturated and unsaturated (but not aromatic) cyclic hydrocarbons (without a heteroatom in the ring), which can be unsubstituted or mono- or polysubstituted.
  • C 3- 4-cycloalkyl represents C3- or C4-cycloalkyl
  • C 3-5 -cycloalkyl represents C3-, C4- or C5-cycloalkyl
  • C 3-6 -cycloalkyl represents C3-, C4-, C5- or C6- cycloalkyl
  • C 3-7 -cycloalkyl represents C3-, C4-, C5-, C6- or C7-cycloalkyl
  • C 3-8 - cycloalkyl represents C3-, C4-, C5-, C6-, C7- or C8-cycloalkyl
  • C 4-5 -cycloalkyl represents CA- or C5-cycloalkyl
  • C 4 -rcycloalkyl represents C4-, C5-, C6- or C7-cycloalkyl
  • C ⁇ -s-cycloalkyl represents C4-
  • alkyl and cycloalkyl radicals are preferably methyl, ethyl, vinyl (ethenyl), propyl, allyl (2- propenyl), 1-propinyl, methylethyl, butyl, 1-methylpropyl, 2-methylpropyl, 1,1- dimethylethyl, pentyl, 1,1-dimethylpropyl, 1,2-dimethylpropyl, 2,2-dimethylpropyl, hexyl, 1 -methyl pentyl, cyclopropyl, 2-methylcyclopropyl, cyclopropyl methyl, cyclobutyl, cyclopentyl, cyclopentyl methyl, cyclohexyl, cycloheptyl,
  • (CH2)3-6 is to be understood as meaning -CH 2 -CHa-CH 2 -, -CH 2 -CH 2 -CH 2 - CH 2 -, -CH 2 -CH 2 -CH 2 -CH 2 - and -CH 2 -CH 2 -CH 2 -CH 2 -CH 2 -, (CH 2 )I -4 is to be understood as meaning -CH 2 -, -CH 2 -CH 2 -, -CH 2 -CH 2 -CH 2 - and -CH 2 -CH 2 -CH 2 -CH 2 -CH 2 -, (CH 2 J 4-5 is to be understood as meaning -CH 2 -CH 2 -CH 2 -CH 2 - and -CH 2 -CH 2 -CH 2 - CH 2 -CH 2 -, etc.
  • aryl radical or group is understood as meaning ring systems with at least one aromatic ring but without heteroatoms even in only one of the rings. Examples are phenyl, naphthyl, fluoranthenyl, fluorenyl, tetralinyl or indanyl, in particular 9H- fluorenyl or anthracenyl radicals, which can be unsubstituted or monosubstituted or polysubstituted.
  • alkyl-aryl is understood as meaning an aryl group (see above) being connected to another atom through an alkyl-group (see above) (preferably a C-M-alkyl), whereas the alkyl is always saturated and linear or branched always refers to the alkyl.
  • alkyl-group preferably a C-M-alkyl
  • a heterocyclyl radical or group is understood as meaning heterocyclic ring systems, saturated or unsaturated ring which contains one or more heteroatoms from the group consisting of nitrogen, oxygen and/or sulfur in the ring and can also be mono- or polysubstituted.
  • heterocyclyls examples include furan, benzofuran, thiophene, benzothiophene, pyrrole, pyridine, pyrimidine, pyrazine, quinoline, isoquinoline, phthalazine, benzo-1 ,2,5-thiadiazole, benzothiazole, indole, benzotriazole, benzodioxolane, benzodioxane, carbazole and quinazoline.
  • alkyl-heterocylyl is understood as meaning a heterocyclyl group (see above) being connected to another atom through an alkyl group (see above) (preferably a C- M -alkyl), whereas the alkyl is always saturated and linear or branched always refers to the alkyl.
  • salt is to be understood as meaning any form of the active compound used according to the invention in which it assumes an ionic form or is charged and is coupled with a counter-ion (a cation or anion) or is in solution.
  • a counter-ion a cation or anion
  • complexes of the active compound with other molecules and ions in particular complexes which are complexed via ionic interactions.
  • physiologically acceptable salt means in the context of this invention any. salt that is physiologically tolerated (most of the time meaning not being toxic- especially not caused by the counter-ion) if used appropriately for a treatment especially if used on or applied to humans and/or mammals.
  • physiologically acceptable salts can be formed with cations or bases and in the context of this invention is understood as meaning salts of at least one of the compounds used according to the invention - usually a (deprotonated) acid - as an anion with at least one, preferably inorganic, cation which is physiologically tolerated - especially if used on humans and/or mammals.
  • the salts of the alkali metals and alkaline earth metals are particularly preferred, and also those with NH4, but in particular (mono)- or (di)sodium, (mono)- or (di)potassium, magnesium or calcium salts.
  • physiologically acceptable salts can also be formed with anions or acids in the context of this invention is understood as meaning salts of at least one of the compounds used according to the invention - usually protonated, for example on the nitrogen - as the cation with at least one anion which are physiologically tolerated - especially if used on humans and/or mammals.
  • the salt formed with a physiologically tolerated acid that is to say salts of the particular active compound with inorganic or organic acids which are physiologically tolerated - especially if used on humans and/or mammals.
  • physiologically tolerated salts of particular acids are salts of: hydrochloric acid, hydrobromic acid, sulfuric acid, methanesulfonic acid, formic acid, acetic acid, oxalic acid, succinic acid, malic acid, tartaric acid, mandelic acid, fumaric acid, lactic acid or citric acid.
  • the compounds of the invention may be in crystalline form or either as free compounds or as solvates and it is intended that those forms are within the scope of the present invention.
  • Methods of solvation are generally known within the art. Suitable solvates are pharmaceutically acceptable solvates.
  • the term "solvate" according to this invention is to be understood as meaning any form of the active compound according to the invention in which this compound has attached to it via non-covalent binding another molecule (most likely a polar solvent) especially including hydrates and alcoholates, e.g. methanolate.
  • the compounds of the invention are also meant to include compounds which differ only in the presence of one or more isotopically enriched atoms.
  • compounds having the present structures except for the replacement of a hydrogen by a deuterium or tritium, or the replacement of a carbon by 13 C- or 14 C-enriched carbon or 15 N-enriched nitrogen are within the scope of this invention.
  • the compounds of formula (I) or their salts or solvates are preferably in pharmaceutically acceptable or substantially pure form.
  • pharmaceutically acceptable form is meant, inter alia, having a pharmaceutically acceptable level of purity excluding normal pharmaceutical additives such as diluents and carriers, and including no material considered toxic at normal dosage levels.
  • Purity levels for the drug substance are preferably above 50%, more preferably above 70%, most preferably above 90%. In a preferred embodiment it is above 95% of the compound of formula (I) or, or of its salts, solvates or prodrugs.
  • the Azepane-or Azocane substituted pyrazoline compounds according to the invention are compounds of general formulas Ia or Ib,
  • X and Y independently represent phenyl, thienyl, naphtyl or pyridyl which groups may be substituted with 1 , 2 or 3 substituents W, which can be the same or different, from the group: branched or linear d- 3 -alkyl or branched or linear d- 3 -alkoxy, phenyl, hydroxy, chloro, bromo, fluoro, iodo, SH, trifluoromethyl, CHF2, CH 2 F, OCHF2, trifluoromethylthio, trifluoromethoxy, methylsulfonyl, carboxyl, trifluoromethylsulfonyl, cyano, carbamoyl, sulfamoyl and acetyl; O-P, with P denominating a prodrug group consisting of aryl, C 8-2 o-alkyl, heteroaryl, C(O)-aryl, C(O)-heteroaryl
  • R 5 , R 6 and R 7 being independently from one another selected from H, F, Cl, Br, I, OH 1 SH, C 1-4 alkyl, C ⁇ alkoxy, CF 3 , CHF 2 , CH 2 F, OCF 3 , a keto-group, NO 2 or NH 2 ; and n being 1 or 2; or
  • R 5 , R 6 and R 7 being independently from one another selected from H, F, Cl, Br, I, OH, SH, C ⁇ alkyl, C ⁇ alkoxy, CF 3 , CHF 2 , CH 2 F, OCF 3 , a keto-group, NO 2 or NH 2 ; and being 1 or 2;
  • the Azepane-or Azocane substituted pyrazoline compounds according to the invention are compounds of general formula M,
  • R 11 , R 12 , R 13 and R 14 independently of one another represent:
  • R 18 representing a hydrogen atom or a branched or linear Ci- 3 -alkyl group
  • R 19 is representing -
  • R 15 , R 16 and R 17 are independently from one another selected from, H, F, Cl, Br, I, OH, SH, CF 3 , CHF 2 , CH 2 F, OCF 3 , a keto-group, NO 2 or NH 2 ; and m being 1 or 2;
  • R 15 , R 16 and R 17 are independently from one another selected from, H 1 F, Cl, Br, I, OH, SH, C 1-4 alkyl, C 1-4 alkoxy, CF 3 , CHF 2 , CH 2 F, OCF 3 , a keto-group, NO 2 or NH 2 ; and m being 1 or 2;
  • the Azepane-or Azocane substituted pyrazoline compounds according to the invention are compounds of general formulas Ma or lib,
  • R 11 , R 12 , R 13 and R 14 independently of one another represent:
  • H branched or linear d- 3 -alkyl or branched or linear d- 3 -alkoxy, phenyl, hydroxy, chloro, bromo, fluoro, iodo, SH, trifluoromethyl, trifluoromethylthio, trifluoromethoxy, CHF 2 , CH 2 F, OCHF 2 , methylsulfonyl, carboxyl, trifluoromethylsulfonyl, cyano, carbamoyl, sulfamoyl and acetyl; O-P, with P denominating a prodrug group consisting of aryl, C 8-2 o-alkyl, heteroaryl, C(O)-aryl, C(O)-heteroaryl, C(O)-Ci -20 -alkyl; - R 18 representing a hydrogen atom or a branched or linear Ci. 3 -alkyl group, while R 19 is representing
  • R 15 , R 16 and R 17 are independently from one another selected from, H, F, Cl, Br, I 1 OH, SH, C 1-4 alkyl, C 1-4 alkoxy, CF 3 , CHF 2 , CH 2 F, OCF 3 , a keto-group, NO 2 or NH 2 ; and m being 1 or 2;
  • R 15 , R 16 and R 17 are independently from one another selected from, H, F, Cl, Br, I, OH, SH, d ⁇ alkyl, C 1-4 alkoxy, CF 3 , CHF 2 , CH 2 F 1 OCF 3 , a keto-group, NO 2 or NH 2 ; and m being 1 or 2;
  • the Azepane-or Azocane substituted pyrazoline compounds of general formulas II, Ma or Mb, according to the invention are characterized in that R 11 , R 12 , R 13 and R 14 independently of one another represent H, CH 3 , C 2 H 5 ,
  • R 11 , R 12 , R 13 and R 14 independently of one another represent H, OH, OCH 3 , £,
  • the Azepane-or Azocane substituted pyrazoline compounds of general formulas II, Ha or Hb, according to the invention are characterized in that
  • R 18 represents H.
  • the Azepane-or Azocane substituted pyrazoline compounds of general formulas II, Ha or Mb, according to the invention are characterized in that
  • R 15 , R 16 and R 17 are independently from one another selected from, H, F, Cl, Br, I, OH, CH 3 , C 2 H 5 , OCH 3 , OCF 3 , Or CF 3 .
  • R 21 , R 22 , R 23 and R 24 independently of one another represent:
  • H branched or linear d- 3 -alkyl or branched or linear Ci -3 -alkoxy, phenyl, hydroxy, chloro, bromo, fluoro, iodo, SH 1 trifluoromethyl, trifluoromethylthio, trifluoromethoxy, methylsulfonyl, carboxyl, trifluoromethylsulfonyl, cyano, carbamoyl, sulfamoyl and acetyl; O-P, with P denominating a prodrug group consisting of aryl, C 8 - 2 o-alkyl, heteroaryl, C(O)-aryl, C(O)-heteroaryl, C(0)-Ci.2o-alkyl;
  • R 28 representing a hydrogen atom or a branched or linear d- 3 -alkyl group
  • R 29 is representing with R 25 , R 26 and R 27 are independently from one another selected from, H, F, Cl, Br, I, OH, SH, CF 3 , CHF 2 , CH 2 F 1 OCF 3 , a keto-group, NO 2 or NH 2 ; p being 1 or 2;
  • R 25 , R 26 and R 27 are independently from one another selected from, H, F, Cl, Br, I, OH, SH 1 Ci -4 alkyl, C ⁇ alkoxy, CF 3 . CHF 2 , CH 2 F, OCF 3 , a keto-group, NO 2 or NH 2 ; p being 1 or 2;
  • the Azepane-or Azocane substituted pyrazoline compounds according to the invention are compounds of general formulas Ilia or IMb,
  • R 21 , R 22 , R 23 and R 24 independently of one another represent:
  • H branched or linear d- 3 -alkyl or branched or linear Ci -3 -alkoxy, phenyl, hydroxy, chloro, bromo, fluoro, iodo, SH, trifluoromethyl, trifluoromethylthio, trifluoromethoxy, methylsulfonyl, carboxyl, trifluoromethylsulfonyl, cyano, carbamoyl, sulfamoyl and acetyl; O-P, with P denominating a prodrug group consisting of aryl, C 8- 2o-alkyl, heteroaryl, C(O)-aryl, C(O)-heteroaryl ) C(O)-Ci -20 -alkyl;
  • R 28 representing a hydrogen atom or a branched or linear Ci -3 -alkyl group
  • R 29 is representing with R 25 , R 26 and R 27 are independently from one another selected from, H, F, Cl, Br, I, OH, SH, C ⁇ alkyl, C 1-4 alkoxy, CF 3 , CHF 2 , CH 2 F, 0CF3, a keto-group, NO 2 or NH 2 ; p being 1 or 2;
  • R 25 , R 26 and R 27 are independently from one another selected from, H, F, Cl 1 Br, I, OH, SH, Ci -4 alkyl, Ci -4 alkoxy, CF 3 , CHF 2 , CH 2 F, OCF 3 , a keto-group, NO 2 or NH 2 ; p being 1 or 2;
  • the Azepane-or Azocane substituted pyrazoline compounds of general formulas III, Ilia or MIb, according to the invention are characterized in that
  • R 21 represents ; O-P, with P denominating a prodrug group consisting of aryl, C 8-2 o-alkyl, heteroaryl, C(O)-aryl, C(O)-heteroaryl, C(O)-Ci -20 -alkyl, while R 22 , R 23 and R 24 independently of one another represent H, CH 3 , C 2 H 5 , C 3 H 7 , OCH 3 , OC 2 H 5 , OH, SH, F, Cl, Br 1 I CF 3 , CHF 2 , CH 2 F, OCF 3 , OCHF 2 ; preferably
  • R 21 represents ; O-P, with P denominating a prodrug group consisting of aryl,
  • R 22 , R 23 and R 24 independently of one another represent H, OH, OCH 3 , F, Cl,
  • R 21 represents ; O-P, with P denominating a prodrug group consisting of aryl, C ⁇ - 20 -alkyl, heteroaryl, CfOJ-aryl, C(O)-heteroaryl, C(O)-C 1-20 -BlKyI, while R 22 , R 23 and R 24 independently of one another represent OH, OCH 3 , F, Cl, Br, I or OCF 3 .
  • the Azepane-or Azocane substituted pyrazoline compounds of general formulas III, Ilia or UIb, according to the invention are characterized in that
  • R 21 , R 22 , R 23 and R 24 independently of one another represent H, CH 3 , C 2 H 5 ,
  • R 21 , R 22 , R 23 and R 24 independently of one another represent H, OH, OCH 3 , F 1
  • R 21 represents H 1 while R 22 , R 23 and R 24 independently of one another represent OH, OCH 3 , F 1 Cl 1 Br, I or OCF 3 .
  • the Azepane-or Azocane substituted pyrazoline compounds of general formulas III, Ilia or 1Mb, according to the invention are characterized in that
  • R 28 represents H.
  • the Azepane-or Azocane substituted pyrazoline compounds of general formulas III, IMa or 1Mb, according to the invention are characterized in that
  • R 25 , R 26 and R 27 are independently from one another selected from, H, F, Cl, Br, I, OH, CH 3 , C 2 H 5 , OCH 3 , OCF 3 , or CF 3 .
  • the Azepane-or Azocane substituted pyrazoline compounds according to the invention are compounds of general formulas IV, IVa or IVb,
  • R 31 , R 32 , R 33 and R 34 independently of one another represent:
  • Ci -3 -alkyl or branched or linear Ci -3 -alkoxy phenyl, hydroxy, chloro, bromo, fluoro, iodo, SH, trifluoromethyl, trifluoromethylthio, trifluoromethoxy, methylsulfonyl, carboxyl, trifluoromethylsulfonyl, cyano, carbamoyl, sulfamoyl and acetyl
  • OP with P denominating a prodrug group consisting of aryl, C ⁇ - 20 -alkyl, heteroaryl, C(O)-aryl, CfOJ-heteroaryl, C(O)-Ci -2 o-alkyl;
  • R 35 , R 36 and R 37 are independently from one another selected from, H, F, Cl,
  • R 38 representing a hydrogen atom or a branched or linear Ci -3 -alkyl group
  • the Azepane-or Azocane substituted pyrazoline compounds according to the invention are compounds of general formulas IVc, IVd or IVe,
  • R 31 , R 32 , R 33 and R 34 independently of one another represent:
  • H branched or linear d- 3 -alkyl or branched or linear Ci -3 -alkoxy, phenyl, hydroxy, chloro, bromo, fluoro, iodo, SH, trifluoromethyl, trifluoromethylthio, trifluoromethoxy, methylsulfonyl, carboxyl, trifluoromethylsulfonyl, cyano, carbamoyl, sulfamoyl and acetyl; O-P, with P denominating a prodrug group consisting of aryl, C 8-2 o-alkyl, heteroaryl, C(O)-aryl, C(O)-heteroaryl, C(O)-Ci.
  • R 35 , R 36 and R 37 are independently from one another selected from, H, F, Cl, Br, I 1 OH 1 SH, d ⁇ alkyl, C 1 ⁇ aIkOXy, CF 3 , CHF 2 , CH 2 F, OCF 3 , a keto-group, NO 2 or NH 2 ;
  • the Azepane-or Azocane substituted pyrazoline compounds of general formulas IV, IVa, IVb, IVc, IVd or IVe, according to the invention are characterized in that
  • R 31 represents ; O-P, with P denominating a prodrug group consisting of aryl, C ⁇ -2 0 -alkyl, heteroaryl, C(O)-aryl, C(O)-heteroaryl, C(O)-Ci -2 o-alkyl > while R 32 , R 33 and R 34 independently of one another represent H, CH 3 , C 2 H 5 , C 3 H 7 , OCH 3 , OC 2 H 5 , OH, SH, F, Cl, Br, I CF 3 , CHF 2 , CH 2 F, OCF 3 , OCHF 2 ;
  • R 31 represents ; O-P, with P denominating a prodrug group consisting of aryl, C 8- 2o-alkyl, heteroaryl, CfOJ-aryl, C(O)-heteroaryl, CfOJ-C ⁇ o-alkyl, while R 32 , R 33 and R 34 independently of one another represent H, OH, OCH 3 , F, Cl, Br, 1, CF 31 CHF 2 Or OCF 3 ;
  • R 31 represents ; O-P, with P denominating a prodrug group consisting of aryl, C 8-2 o-alkyl, heteroaryl, C(O)-aryl, C(O)-heteroaryl, C(O)-Ci -2 o-alkyl, while R 32 , R 33 and R 34 independently of one another represent OH, OCH 3 , F, Cl, Br, I or OCF 3 .
  • the Azepane-or Azocane substituted pyrazoline compounds of general formulas IV, IVa, IVb, IVc, IVd or IVe, according to the invention are characterized in that R 31 , R 32 , R 33 and R 34 independently of one another represent H, CH 3 , C 2 H 5 ,
  • R 31 , R 32 , R 33 and R 34 independently of one another represent H, OH, OCH 31 JE,
  • R 31 represents H, while R 32 , R 33 and R 34 independently of one another represent OH, OCH 3 , F, Cl, Br, I or OCF 3 .
  • the Azepane-or Azocane substituted pyrazoline compounds of general formulas IV 1 IVa, IVb, IVc, IVd or IVe, according to the invention are characterized in that
  • R 38 represents H.
  • the Azepane-or Azocane substituted pyrazoline compounds of general formulas IV, IVa, IVb, IVc, IVd or IVe, according to the invention are characterized in that
  • R 35 , R 36 and R 37 are independently from one another selected from, H, F, Cl, Br, I, OH, CH 3 , C 2 H 5 , OCH 3 , OCF 3 , or CF 3 .
  • Azepane-or Azocane substituted pyrazoline compounds of general formulas IV, IVa, IVb according to the invention are selected from the group consisting of:
  • the Azepane-or Azocane substituted pyrazoline compounds of general formulas IVc, IVd or IVe, according to the invention are selected from the group consisting of:
  • the present invention also provides a process for the manufacture of substituted pyrazoline compounds of general formula I according to the invention, characterized in that at least one benzaldehyde compound of general formula V
  • G represents an OR group with R being a branched or unbranched Ci- 6 alkyl radical or G represents an O K group with K being a cation, to yield a compound of general formula (VII)
  • X and Y have the meaning as given above, which is optionally isolated and/or optionally purified, and optionally transferred under inert atmosphere to a compound of general formula (Xl) via the reaction with an activating agent
  • substituents X and Y have the meaning given above and A represents a leaving group, said compound being optionally isolated and/or optionally purified, and at least one compound of general formula (Xl) is reacted with a compound of general formula XII or XIIa
  • the reaction of the benzaldehyde compound of general formula V with a pyruvate compound of general formula Vl is preferably carried out in the presence of at least one base, more preferably in the presence of an alkali metal hydroxide such as sodium hydroxide or potassium hydroxide or an alkali metal methoxide such as sodium methoxide, as described, for example, in Synthetic communications, 26(11 ), 2229-33, (1996).
  • an alkali metal hydroxide such as sodium hydroxide or potassium hydroxide
  • an alkali metal methoxide such as sodium methoxide
  • sodium pyruvate may be used as the pyruvate compound.
  • said reaction is carried out in a protic reaction medium such as a Ci -4 alkyl alcohol or mixtures of these.
  • reaction temperature as well as the duration of the reaction may vary over a broad range. Preferred reaction temperatures range from -10 0 C to the boiling point of the reaction medium. Suitable reaction times may vary for example from several minutes to several hours. _
  • reaction of the benzaldehyde compound of general formula V with a pyruvate compound of general formula Vl is carried out under acid catalysed conditions, more preferably by refluxing the mixture in dichloromethane in the presence of copper(ll)trifluoromethanesulfonate as described, for example, in Synlett, (1 ), 147-149, 2001.
  • acid catalysed conditions more preferably by refluxing the mixture in dichloromethane in the presence of copper(ll)trifluoromethanesulfonate as described, for example, in Synlett, (1 ), 147-149, 2001.
  • the respective description is hereby incorporated by reference and forms part of the disclosure.
  • reaction of the compound of general formula (VII) with an optionally substituted phenyl hydrazin of general formula (VIII) is preferably carried out in a suitable reaction medium such as Ci-4-alcohols or ethers such as dioxane or tetrahydrofurane or mixtures of at least two of these afore mentioned compounds.
  • a suitable reaction medium such as Ci-4-alcohols or ethers such as dioxane or tetrahydrofurane or mixtures of at least two of these afore mentioned compounds.
  • said reaction may be carried out in the presence of an acid, whereby the acid may be organic such as acetic acid and/or inorganic such as hydrochloric acid.
  • reaction may also be carried out in the presence of a base such as piperidine, piperazine, sodium hydroxide, potassium hydroxide, sodium methoxide or sodium ethoxide, or a mixture of at least two of these bases may also be used.
  • a base such as piperidine, piperazine, sodium hydroxide, potassium hydroxide, sodium methoxide or sodium ethoxide, or a mixture of at least two of these bases may also be used.
  • Reaction temperature as well as the duration of the reaction may vary over a broad range. Suitable reaction temperatures range from room temperature, i.e. approximately 25 0 C to the boiling point of the reaction medium. Suitable reaction times may vary for example from several minutes to several hours.
  • the carboxylic group of the compound of general formula (IX) may be activated for further reactions by the introduction of a suitable leaving group according to conventional methods well known to those skilled in the art.
  • the compounds of general formula (IX) are transferred into an acid chloride, an acid anhydride, a mixed anhydride, a Ci -4 alkyl ester, an activated ester such as p- nitrophenylester.
  • Other well known methods for the activation of acids include the activation with N.N-dicyclohexylcarbodiimide or benzotriazol-N- oxotris(dimethylamino) phosphonium hexafluorophosphate (BOP)).
  • said activated compound of general formula (Xl) is an acid chloride, it is preferably prepared by reaction of the corresponding acid of general formula (IX) with thionyl chloride or oxalyl chloride, whereby said chlorinating agent is also used as the solvent.
  • an additional solvent may be used.
  • Suitable solvents include hydrocarbons such as benzene, toluene or xylene, halogenated hydrocarbons such as dichloromethane, chloroform or carbon tetrachloride, ethers such as diethyl ether, dioxane, tetrahydrofurane or dimethoxyethane. Mixtures of two or more solvents from one class or two or more solvents from different classes may also be used.
  • Preferred reaction temperature range from 0° C to the boiling point of the solvent and reaction times from several minutes to several hours.
  • said activated compound of general formula (Xl) is a mixed anhydride
  • said anhydride may preferably be prepared, for example, by reaction of the corresponding acid of general formula (IX) with ethyl chloroformiate in the presence of a base such as triethylamine or pyridine, in a suitable solvent.
  • substituted pyrazoline compounds of general formula (I) themselves are obtained in form of a mixture of stereoisomers, particularly enantiomers or diastereomers, said mixtures may be separated by standard procedures known to those skilled in the art, e.g. chromatographic methods or fractunalized crystallization with chiral reagents. It is also possible to obtain pure stereoisomers via stereoselective synthesis.
  • the present invention also provides a process for the preparation, of salts of substituted pyrazoline compounds of general formula (I) and stereoisomers thereof, wherein at least one compound of general formula (I) having at least one basic group is reacted with at least one inorganic and/or organic acid, preferably in the presence of a suitable reaction medium.
  • suitable reaction media include, for example, any of the ones given above.
  • Suitable inorganic acids include hydrochloric acid, hydrobromic acid, phosphoric acid, sulfuric acid, nitric acid
  • suitable organic acids are e.g. citric acid, maleic acid, fumaric acid, tartaric acid, or derivatives thereof, p-toluenesulfonic acid, methanesulfonic acid or camphersulfonic acid.
  • the present invention also provides a process for the preparation of salts of substituted pyrazoline compounds of general formula (I) or stereoisomers thereof, wherein at least one compound of general formula (I) having at least one acidic group is reacted with one or more suitable bases, preferably in the presence of a suitable reaction medium.
  • suitable bases are e.g. hydroxides, carbonates or alkoxides, which include suitable cations, derived e.g. from alkaline metals, alkaline earth metals or organic cations, e.g. [NH n R-Mi] + , wherein n is 0, 1 , 2, 3 or 4 and R represents a branched or unbranched Ci -4 -alkyl-radical.
  • suitable reaction media are, for example, any of the ones given above.
  • Solvates preferably hydrates, of the substituted pyrazoline compounds of general formula (I), of corresponding stereoisomers, of corresponding N-oxides or of corresponding salts thereof may also be obtained by standard procedures known to those skilled in the art.
  • Substituted pyrazoline compounds of general formula I which comprise nitrogen- atom containing saturated, unsaturated or aromatic rings may also be obtained in the form of their N-oxides by methods well known to those skilled in the art.
  • substituted pyrazoline compounds as used herein is to be understood as encompassing derivatives such as ethers, esters and complexes of these compounds as well.
  • derivatives as used in this application is defined here as meaning a chemical compound having undergone a chemical derivation starting from an acting (active) compound to change (ameliorate for pharmaceutical use) any of its physico-chemical properties, especially a so-called prodrug, e.g. their esters and ethers. Examples of well known methods of producing a prodrug of a given acting compound are known to those skilled in the art and can be found e.g. in Krogsgaard-Larsen et al., Textbook of Drugdesign and Discovery, Taylor & Francis (April 2002). The respective description is hereby incorporated by reference and forms part of the disclosure.
  • Azepane- or Azocane-substituted pyrazoline compounds of general formula I given above, their stereoisomers, corresponding N-oxides, corresponding salts thereof and corresponding solvates are toxicologically acceptable and are therefore suitable as pharmaceutical active substances for the preparation of medicaments.
  • the Azepane- or Azocane-substituted pyrazoline compounds of general formula I given above, stereoisomers thereof, N-oxides thereof, corresponding salts and corresponding solvates have a high affinity to cannabinoid receptors, particularly cannabinoid 1 (CBi)-receptors, i.e. they are selective ligands for the (CBi)-receptor and act as modulators, e.g. antagonists, inverse agonists or agonists, on these receptors.
  • these pyrazoline compounds show little or no development of tolerance during treatment, particularly with respect to food intake, i.e.
  • inventively used pyrazoline compounds will again show the desired effect.
  • the positive influence on the body weight is found to continue.
  • these Azepane- or Azocane-substituted pyrazoline compounds show relatively weak Herg channel affinity, thus a low risk of prolongation of the QT-interval is to be expected for these compounds.
  • the inventively used 4-subsituted pyrazoline compounds are distinguished by a broad spectrum of beneficial effects, while at the same time showing relatively little undesired effects, i.e. effects which do not positively contribute to or even interfere with the well being of the patient.
  • an other aspect of the present invention relates to a medicament comprising at least one Azepane- or Azocane-substituted pyrazoline compound of general formula I, optionally in form of one of its stereoisomers, preferably enantiomers or diastereomers, a racemate or in form of a mixture of at least two of its stereoisomers, preferably enantiomers and/or diastereomers, in any mixing ratio, or a corresponding N-oxide thereof, or a physiologically acceptable salt thereof, or a corresponding solvate thereof, and optionally at least one physiologically acceptable auxiliary agent.
  • said medicament is suitable for the modulation (regulation) of cannabinoid-receptors, preferably cannabinoid 1 (CBi) receptors, for the prophylaxis and/or treatment of disorders of the central nervous system, disorders of the immune system, disorders of the cardiovascular system, disorders of the endocrinous system, disorders of the respiratory system, disorders of the gastrointestinal tract or reproductive disorders.
  • cannabinoid-receptors preferably cannabinoid 1 (CBi) receptors
  • Particularly preferably said medicament is suitable for the prophylaxis and/or treatment of psychosis.
  • said medicament is suitable for the prophylaxis and/or treatment of food intake disorders, preferably bulimia, anorexia, cachexia, obesity and/or type Il diabetus mellitus (non-insuline dependent diabetes mellitus), more preferably obesity.
  • the inventive medicament also seems to be active in the prophylaxis and/or treatment of appetency disorders, e.g. the pyrazoline compounds of general formula I also reduce the desire for sweets.
  • said medicament is suitable for the prophylaxis and/or treatment of cancer, preferably for the prophylaxis and/or treatment of one or more types of cancer selected from the group consisting of brain cancer, bone cancer, lip cancer, mouth cancer, esophageal cancer, stomach cancer, liver cancer, bladder _ cancer, pancreas cancer, ovary cancer, cervical cancer, lung cancer, breast cancer, skin cancer, colon cancer, bowel cancer and prostate cancer, more preferably for the prophylaxis and/or treatment of one or more types of cancer selected from the group consisting of colon cancer, bowel cancer and prostate cancer.
  • Particularly preferably said medicament is suitable for the prophylaxis and/or treatment of alcohol abuse and/or alcohol addiction, nicotine abuse and/or nicotine addiction, drug abuse and/or drug addiction and/or medicament abuse and/or medicament addiction, preferably drug abuse and/or drug addiction and/or nicotine abuse and/or nicotine addiction.
  • Medicaments and/or drugs which are frequently the subject of misuse include opioids, barbiturates, cannabis, cocaine, amphetamines, phencyclidine, hallucinogens and benzodiazepines.
  • the medicament is also suitable for the prophylaxis and/or treatment of one or more disorders selected from the group consisting of bone disorders, preferably osteoporosis (e.g. osteoporosis associated with a genetic predisposition, sex hormone deficiency, or ageing), cancer-associated bone disease or Paget ' s disease of bone; schizophrenia, anxiety, depression, epilepsy, neurodegenerative disorders, cerebellar disorders, spinocerebellar disorders, cognitive disorders, cranial trauma, head trauma, stroke, panic attacks, peripheric neuropathy, inflammation, glaucoma, migraine, Morbus Parkinson, Morbus Huntington, Morbus Alzheimer, Raynaud's disease, tremblement disorders, compulsive disorders, senile dementia, thymic disorders, tardive dyskinesia, bipolar disorders, medicament-induced movement disorders, dystonia, endotoxemic shock, hemorrhagic shock, hypotension, insomnia, immunologic disorders, sclerotic plaques, vomiting, diarr
  • Another aspect of the present invention is the use of at least one Azepane- or Azocane-substituted pyrazoline compound of general formula I given above as suitable active substances, optionally in form of one of the stereoisomers, preferably enantiomers or diastereomers, a racemate or in form of a mixture of at least two of- the stereoisomers, preferably enantiomers and/or diastereomers, in any mixing ratio, or a corresponding N-oxide thereof, or a corresponding salt thereof, or a corresponding solvate thereof, and optionally one or more pharmaceutically acceptable excipients, for the preparation of a medicament for the modulation of cannabinoid-receptors, preferably cannabinoid 1 (CBi) receptors, for the prophylaxis and/or treatment of disorders of the central nervous system, disorders of the immune system, disorders of the cardiovascular system, disorders of the endocrinous system, disorders of the respiratory system, disorders of the gastrointestinal tract or reproductive disorders.
  • At least one of the respective Azepane- or Azocane-substituted pyrazoline compounds optionally in form of one of the stereoisomers, preferably enantiomers or diastereomers, a racemate or in form of a mixture of at least two of the stereoisomers, preferably enantiomers and/or diastereomers, in any mixing ratio, or a corresponding N-oxide thereof, or a corresponding salt thereof, or a corresponding solvate thereof, and optionally one or more pharmaceutically acceptable excipients, for the preparation of a medicament for the prophylaxis and/or treatment of psychosis.
  • At least one of the respective Azepane- or Azocane-substituted pyrazoline compounds optionally in form of one of the stereoisomers, preferably enantiomers or diastereomers, a racemate or in form of a mixture of at least two of the stereoisomers, preferably enantiomers and/or diastereomers, in any mixing ratio, or a corresponding N-oxide thereof, or a corresponding salt thereof, or a corresponding solvate thereof, and optionally one or more pharmaceutically acceptable excipients, for the preparation of a medicament for the prophylaxis and/or treatment of food intake disorders, preferably bulimia, anorexia, cachexia, obesity and/or type Il diabetus mellitus (non-insuline dependent diabetes mellitus), more preferably obesity. Also particularly preferred is the use of at least one of the pyrazoline compounds as defined herein and optionally one or more pharmaceutically acceptable excipients, for the preparation of
  • the metabolic syndrome and definitions thereof are described in detail by Eckel et al., The Lancet, Vol. 365 (2005), 1415-1428, included herewith by reference.
  • One of the respective definitions was established by the WHO in 1998 (as described in Alberti et al., Diabet. Med. 1998, 15, pages 539-53, the respective description thereof is herewith incorporated by reference and forms part of the present disclosure).
  • the other, more widely accepted, definition of the metabolic syndrome was established by the Adult Treatment Panel (ATP III) of the US National Cholesterol Education Program (NCEP) in 2001, as described in JAMA 2001; 285; 2486-97, the respective description thereof is herewith incorporated by reference and forms part of the present disclosure.
  • the metabolic syndrome is characterized by an interaction of several physiological parameters such as triglycerides, lipids, blood pressure, glucose levels and insuline levels.
  • Another aspect of the invention is the use of one or more pyrazoline compounds as defined herein for the manufacture of a medicament for improvent of cardiovascular and/or metabolic risk factors, such as one or more of the following factors:
  • Elevated triglycerides whereby elevated levels of triglycerides are preferably understood as being > 150 mg/dl,
  • Low HDL cholesterol whereby low levels of HDL cholesterol are preferably understood as being ⁇ 40 mg/dl in men and ⁇ 50 mg/dl in women
  • Hypertension whereby hypertension is preferably understood as being > 130/85 mmHg
  • Impaired fasting glucose whereby impaired fasting glucose levels are preferably understood as being > 110 mg/dl,
  • Another aspect of the invention is the use of one or more pyrazoline compounds as defined herein for the manufacture of a medicament for the treatment of the weight independent aspects of metabolic syndrome.
  • Another aspect of the invention is a method for improving cardiovascular and/or metabolic risk factors, such as one or more of the following factors:
  • Elevated triglycerides whereby elevated levels of triglycerides are preferably understood as being > 150 mg/dl,
  • Low HDL cholesterol whereby low levels of HDL cholesterol are preferably understood as being ⁇ 40 mg/dl in men and ⁇ 50 mg/dl in women,
  • Hypertension whereby hypertension is preferably understood as being > 130/85 mmHg,
  • Impaired fasting glucose whereby impaired fasting glucose levels are preferably understood as being > 110 mg/dl,
  • Dyslipidemia in a subject, preferably a human.
  • Another aspect of the invention is a method for treating of the weight independent aspects of metabolic syndrome.
  • At least one of the respective Azepane- or Azocane-substituted pyrazoline compounds optionally in form of one of the stereoisomers, preferably enantiomers or diastereomers, a racemate or in form of a mixture of at least two of the stereoisomers, preferably enantiomers and/or diastereomers, in any mixing ratio, or a corresponding N-oxide thereof, or a corresponding salt thereof, or a corresponding solvate thereof, and optionally one or more pharmaceutically acceptable excipients, for the preparation of a medicament for the prophylaxis and/or treatment of cancer, preferably for the prophylaxis and/or treatment of one or more types of cancer selected from the group consisting of brain cancer, bone cancer, lip cancer, mouth cancer, esophageal cancer, stomach cancer, liver cancer, bladder cancer, pancreas cancer, ovary cancer, cervical cancer, lung cancer, breast cancer, skin cancer, colon cancer, bowel cancer and
  • At least one of the respective Azepane- or Azocane-substituted pyrazoline compounds optionally in form of one of the stereoisomers, preferably enantiomers or diastereomers, a racemate or in form of a mixture of at least two of the stereoisomers, preferably enantiomers and/or diastereomers, in any mixing ratio, or a corresponding N-oxide thereof, or a corresponding salt thereof, or a corresponding solvate thereof, and optionally one or more pharmaceutically acceptable excipients, for the preparation of a medicament for the prophylaxis and/or treatment of alcohol abuse and/or alcohol addiction, nicotine abuse and/or nicotine addiction, drug abuse and/or drug addiction and/or medicament abuse and/or medicament addiction, preferably drug abuse and/or drug addiction and/or nicotine abuse and/or nicotine addiction.
  • Medicaments/drugs which are frequently the subject of misuse include opioids, barbiturates, cannabis, cocaine, amphetamines,
  • At least one of the respective Azepane- or Azocane- substituted pyrazoline compounds optionally in form of one of the stereoisomers, preferably enantiomers or diastereomers, a racemate or in form of a mixture of at least two of the stereoisomers, preferably enantiomers and/or diastereomers, in any mixing ratio, or a corresponding N-oxide thereof, or a corresponding salt thereof, or a corresponding solvate thereof, and optionally one or more pharmaceutically acceptable excipients, for the preparation of a medicament for the prophylaxis and/or treatment of one or more disorders selected from the group consisting of bone disorders, preferably osteoporosis (e.g.
  • the medicament according to the present invention may be in any form suitable for the application to humans and/or animals, preferably humans including infants, children and adults and can be produced by standard procedures known to those skilled in the art.
  • the medicament can be produced by standard procedures known to those skilled in the art, e.g. from the table of contents of "Pharmaceutics: The Science of Dosage Forms", Second Edition, Aulton, M. E. (ED. Churchill Livingstone, Edinburgh (2002); “Encyclopedia of Pharmaceutical Technology", Second Edition, Swarbrick, J. and Boylan J.C. (Eds.), Marcel Dekker, Inc. New York (2002); "Modern Pharmaceutics", Fourth Edition, Banker G. S. and Rhodes CT.
  • composition of the medicament may vary depending on the route of administration.
  • the medicament of the present invention may for example be administered parentally in combination with conventional injectable liquid carriers, such as water or suitable alcohols.
  • conventional pharmaceutical excipients for injection such as stabilizing agents, solubilizing agents, and buffers, may be included in such injectable compositions.
  • These medicaments may for example be injected intramuscularly, intraperitoneally, or intravenously.
  • Medicaments according to the present invention may also be formulated into orally administrable compositions containing one or more physiologically compatible carriers or excipients, in solid or liquid form.
  • These compositions may contain conventional ingredients such as binding agents, fillers, lubricants, and acceptable wetting agents.
  • the compositions may take any convenient form, such as tablets, pellets, granules, capsules, lozenges, aqueous or oily solutions, suspensions, emulsions, or dry powdered forms suitable for reconstitution with water or other suitable liquid medium before use, for immediate or retarded release.
  • the multiparticulate forms, such as pellets or granules may e.g. be filled into a capsule, compressed into tablets or suspended in a suitable liquid.
  • Suitable controlled release formulations, materials and methods for their preparation are known from the prior art, e.g. from the table of contents of "Modified-Release Drug Delivery Technology", Rathbone, M.J. Hadgraft, J. and Roberts, M.S. (Eds.), Marcel Dekker, Inc., New York (2002); "Handbook of Pharmaceutical Controlled Release Technology”, Wise, D.L. (Ed.), Marcel Dekker, Inc. New York, (2000); "Controlled Drug Delivery", VoI, I, Basic Concepts, Bruck, S.D. (Ed.), CRD Press Inc., Boca Raton (1983) y de Takada, K.
  • Medicaments according to the present invention may also comprise an enteric « coating, so that their dissolution is dependent on pH-value. Due to said coating the medicament can pass the stomach undissolved and the respective nitro-subsituted phenyl-piperazine compound is liberated in the intestinal tract.
  • the enteric coating is soluble at a pH value of 5 to 7.5. Suitable materials and methods for the preparation are known from the prior art.
  • the medicaments according to the present invention may contain 1-60 % by weight of one or more Azepane- or Azocane-substituted pyrazoline compounds as defined herein and 40-99 % by weight of one or more auxiliary substances (additives).
  • liquid oral forms for administration may also contain certain additives such as sweeteners, flavoring, preservatives, and emulsifying agents.
  • Non-aqueous liquid compositions for oral administration may also be formulated, containing edible oils. Such liquid compositions may be conveniently encapsulated in e.g., gelatin capsules in a unit dosage amount.
  • compositions of the present invention may also be administered topically or via a suppository.
  • the daily dosage for humans and animals may vary depending on factors that have their basis in the respective species or other factors, such as age, sex, weight or degree of illness and so forth.
  • the daily dosage for humans may preferably be in the range fromi to 2000, preferably 1 to 1500, more preferably 1 to 1000, even more preferably 1 to 150 milligrams of active substance to be administered during one or several intakes per day.
  • the present invention is illustrated below with the aid of examples. These illustrations are given solely by way of example and do not limit the general spirit of the present invention.
  • step a) 4-(4-substituted-phenyl)-2-oxo-3-butenoic acid obtained according to step a) (60 mmoles), 2,4-dichlorophenylhydrazine or 2-chlorophenylhydrazine hydrochloride (60 mmoles) and glacial acetic acid (200 ml_) are mixed under a nitrogen atmosphere and heated to reflux for 4 hours, cooled down to room temperature (approximately 25 0 C) and given into ice-water, whereby a sticky mass is obtained, which is extracted with methylene chloride.
  • step (b) 5-(4-substituted-phenyl)-1-(2,4-dichlorophenyl)-4,5-dihydro-pyrazole-3-carboxy- Hc acid (15 mmols) obtained according to step (b) is dissolved in 120 ml_ of dry toluene and thionyl chloride (18 mmols) is added. The mixture is heated to 80 0 C for 2.5 hours. The solvent is removed under reduced pressure and the resulting crude residue is used without any further purification.
  • the compound can be purified by crystallization of JJs hydrochloride form, prepared with a solution of 2 N HCI in ether or 2.8 N HCI in ethanol.
  • step c) If the starting material in step c) is a chiral acid.
  • inventive pyrazoline compounds are particularly suitable for regulating the CBrReceptor.
  • Substances with affinity for cannabinoid receptors are known to produce a wide range of pharmacological effects. It is also known that intravenous administration of a substance with affinity for cannabinoid receptors in mice produces analgesia , hypothermia, sedation and catalepsy. Individually, none of these effects can be considered as proof that a tested substance has affinity for cannabinoid-receptors, since all of these effects are common for various classes of centrally active agents. However, substances, which show all of these effects, i.e. substances that are active in this so-called tetrad model are considered to have affinity for the cannabinoid receptors. It has further been shown that cannabinoid receptor antagonists are higly effective in blocking the effects of a cannabinoid agonist in the mouse tetrad model s
  • mice with a weight of 20-30 g Male NMRI mice with a weight of 20-30 g (Harian, Barcelona, Spain) are used in all of the following experiments.
  • mice are acclimatized to the experimental setting.
  • Pre-Treatment control values are determined for analgesia hot plate latency (in seconds), rectal temperature, sedation and catalepsy.
  • mice In order to determine the agonistic activty of the substance to be tested, the mice are injected intravenously with the substance to be tested or the vehicle alone. 15 minutes after injection, latency in hot plate analgesia is measured. Rectal temperature, sedation and catalepsy are measured 20 minutes after injection.
  • Hot plate analgesia The hot plate analgesia is determined according to the method described in Woolfe D. et al. ,,The evaluation of analgesic action of pethidine hydrochloride (Demerol)", J. Pharmacol. Exp. Ther. 80, 300-307,1944. The respective description is hereby incorporated by reference and forms part of the present disclosure. _
  • mice are placed on a hot plate (Harvard Analgesimeter) at 55 ⁇ 0.5 0 C until they show a painful sensation by licking their paws or jumping and the time for these sensations to occur is recorded. This reading is considered the basal value (B).
  • B basal value
  • PC cut-off time
  • mice Fifteen minuts after the administration of the substance to be tested, the mice are again placed on the hot plate and the afore described procedure is repeated. This period is called the post-treatment reading (PT).
  • PT post-treatment reading
  • the degree of analgesia is calculated from the formula :
  • % MPE of Analgesia ( PT- B) / (PC-B) x 100
  • Sedation and ataxia is determined according to the method described in Desmet L. K. C. et al. ..Anticonvulsive properties of Cinarizine and Flunarizine in Rats and Mice", Arzneim. -Forsch. (Frug Res) 25, 9, 1975.
  • the respective description is hereby incorporated by reference and forms part of the present disclosure.
  • the chosen scoring system is
  • the percentage of sedation is determined according to the formula:
  • % of sedation arithmetic mean / 3 X 100
  • the base-line rectal temperatures are determined with a thermometer (YeIIo Springs Instruments Co., Panlabs) and a thermistor probe inserted to 25mm before the administration of the substance to be tested. Rectal temperature is again measured 20 minutes after the administration of the substances to be tested. The temperature difference is calculated for each animal, whereby differences of ⁇ -2 0 C are considered to represent activity.
  • Catalepsy is determined according to the method described in Alpermann H. G. et al. ..Pharmacological effets of Hoe 249: A new potential antidepressant", Drugs Dev. Res. 25, 267-282. 1992. The respective description is hereby incorporated by reference and forms part of the present disclosure.
  • the cataleptic effect of the substance to be tested is evaluated according to the duration of catalepsy, whereby the animals are placed head downwards with their kinlegs upon the top of the wooden block.
  • the chosen scoring system is:
  • the percentage of catalepsy is determined according ot the following formula:
  • % Catalepsy arithmetic mean / 6 X 100
  • CB1 Chinese hamster ovary (CHO) cells stably expressing recombinant human cannabinoid 1 receptor (CB1 ) were cultured in nutrient mixture Ham's F 12 supplemented with 10 % heat-inactivated fetal bovine serum, 2 mM L-glutamine, 50 U/ml penicillin, 50 U/ml streptomycin and 0.5 mg/ml geneticin. In order to obtain cells, culture flasks were washed twice with phosphate buffered saline and scraped. Then, cells were collected by centrifugation (200 x g, 10 min) and stored dry at -8O 0 C.
  • Cells were homogenized in ice-cold 20 mM HEPES, 10 mM EDTA (pH 7.5) and centrifuged at 40,000 x g for 15 min at 4°C. The pellet was resuspended in 20 mM HEPES, 0.1 mM EDTA (pH 7.5) and centrifuged for 15 min at 4°C. The final pellet was resuspended in 20 mM HEPES, 0.1 mM EDTA (pH 7.5), and divided in aliquots and stored at -8O 0 C until use.
  • the reaction was performed in 96-well plates. Membranes (15 Dg protein/well) were incubated for 60 min at 30 0 C in buffer (50 mM HEPES, 100 mM KCI, 5 mM MgCI 2 , 1 mM EDTA, 0.1 % wt/vol bovine serum albumin, 5 ⁇ M GDP, saponin (10 ⁇ g/ml), 0.5 nM [ 35 S]GTPyS, pH 7.4) with compound at 1 ⁇ M final concentration in either the absence or presence of dose response curve of agonist WIN 55,212-2 between 3 nM and 3 ⁇ M.
  • buffer 50 mM HEPES, 100 mM KCI, 5 mM MgCI 2 , 1 mM EDTA, 0.1 % wt/vol bovine serum albumin, 5 ⁇ M GDP, saponin (10 ⁇ g/ml), 0.5 nM [ 35 S]GTPyS, pH 7.4
  • Normally handled rats were habituated to a reversed cycle 12/12h, and the ⁇ compound as well as saline was acutely orally administered. After administration the cumulated food intake (g) was measured at 6h and 23 h. Following that the difference in body weight between control and compound treated anmimals was measured.
  • Fig. 1 shows the results for 10 mg/kg of the compound according to example 2.
  • the number of rats used was 11 for saline and 12 for the compound.

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Abstract

La présente invention concerne des composés de pyrazoline substitués par Azépane ou Azocane, des procédés de préparation, des médicaments comprenant ces composés ainsi que leur utilisation dans la préparation d'un médicament pour le traitement des êtres humains et des animaux.
PCT/EP2006/007412 2005-07-15 2006-07-16 Composés de pyrazoline substitués par azépane ou azocane, leur préparation et leur utilisation comme médicaments WO2007131538A1 (fr)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
EP05384018.7 2005-07-15
EP05384018A EP1743888A1 (fr) 2005-07-15 2005-07-15 Composés de pyrazoline subsitués par un groupe carbonyle utiles comme modulateurs du recepteur CB1
US70543305P 2005-08-05 2005-08-05
US60/705,433 2005-08-05
EP06008612.1 2006-04-26
EP06008612A EP1849776A1 (fr) 2006-04-26 2006-04-26 Dérivés de la pyrazoline azépane- ou azocane-substituées, leur préparation et utilisation comme médicaments

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US9714230B2 (en) 2012-12-06 2017-07-25 Celgene Quantical Research, Inc. Histone demethylase inhibitors
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