Classifications

• • G—PHYSICS
  • G01—MEASURING; TESTING
  • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
  • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
  • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
  • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
  • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
  • G01N33/6893—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids related to diseases not provided for elsewhere
• • G—PHYSICS
  • G01—MEASURING; TESTING
  • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
  • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
  • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
  • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
• • G—PHYSICS
  • G01—MEASURING; TESTING
  • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
  • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
  • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
• • G—PHYSICS
  • G01—MEASURING; TESTING
  • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
  • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
  • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
  • G01N33/483—Physical analysis of biological material
  • G01N33/487—Physical analysis of biological material of liquid biological material
• • G—PHYSICS
  • G01—MEASURING; TESTING
  • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
  • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
  • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
  • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
  • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
  • G01N33/575—Immunoassay; Biospecific binding assay; Materials therefor for cancer
• • G—PHYSICS
  • G01—MEASURING; TESTING
  • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
  • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
  • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
  • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
  • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
  • G01N33/575—Immunoassay; Biospecific binding assay; Materials therefor for cancer
  • G01N33/5758—Immunoassay; Biospecific binding assay; Materials therefor for cancer involving compounds serving as markers for tumours, cancers or neoplasias, e.g. cellular determinants, receptors, heat shock/stress proteins, A-protein, oligosaccharides or metabolites
  • G01N33/57585—Immunoassay; Biospecific binding assay; Materials therefor for cancer involving compounds serving as markers for tumours, cancers or neoplasias, e.g. cellular determinants, receptors, heat shock/stress proteins, A-protein, oligosaccharides or metabolites involving compounds identifiable in body fluids
• • G—PHYSICS
  • G01—MEASURING; TESTING
  • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
  • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
  • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
  • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
  • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
  • G01N33/689—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids related to pregnancy or the gonads
• • G—PHYSICS
  • G01—MEASURING; TESTING
  • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
  • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
  • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
  • G01N2333/52—Assays involving cytokines
  • G01N2333/53—Colony-stimulating factor [CSF]
  • G01N2333/535—Granulocyte CSF; Granulocyte-macrophage CSF

Definitions

• This invention relates to a naturally occurring soluble fragment of the receptor for human M-CSF which is capable of binding M-CSF, methods of using this soluble fragment for therapy, and methods of diagnosing diseases and conditions by detecting this soluble fragment in patient samples.
• Macrophage colony-stimulating factor regulates the survival, proliferation, and differentiation of macrophages and their precursors and osteoclasts and their precursors.
• M-CSF activity is tightly controlled through mechanisms regulating gene expression of M-CSF and its membrane-bound receptor (M-CSFR), as well as by receptor-mediated endocytosis, metabolic processing, and inhibition of downstream signaling.
• M-CSF is expressed in stromal cells, osteoblasts, and other cells. It is also expressed in breast, uterine, and ovarian tumor cells. The extent of expression in these tumors correlates with high grade and poor prognosis (Kacinski Ann. Med. 27: 79-85 (1995); Smith et al., Clin. Cancer Res. 1 : 313-25 (1995)). In breast carcinomas, M-CSF expression is prevalent in invasive tumor cells as opposed to the intraductal (pre-invasive) cancer (Scholl et al., J. Natl. Cancer Inst. 86: 120-6 (1?>94)). In addition, M-CSF is shown to promote progression of mammary tumors to malignancy (Lin et al., J. Exp. Med. 93: 727-39 (2001)).
• Human membrane-bound M-CSF receptor is encoded by the c-fms proto- oncogene and is a protein tyrosine-kinase transmembrane receptor, a Type I membrane protein, expressed in bone marrow and in differentiated blood mononuclear cells.
• the M- CSF receptor belongs to the Tyr protein kinase family and the CSF-I /PDGF receptor subfamily. It contains five Ig-like C2-type (immunoglobulin-like) domains, one •transmembrane domain, and an intracellular interrupted Src-related domain with two kinase domains.
• M-CSF typically binds to its receptor in order to exert a biological effect.
• M-CSF binding to c-fmsleads to homo-dimerization of the receptor which activates the cytoplasmic kinase domain, causing autophosphorylation and phosphorylation of other cellular proteins.
• These events initiate a cascade of signal transduction pathways including the JAK/STAT, P13K, and ERK pathways that result in a variety of cellular responses: mitosis, secretion of cytokines, membrane ruffling, and regulation of transcription of M-CSFR.
• the materials and methods of the present invention fulfill the aforementioned and other related needs in the art.
• CSF-I human M-CSF
• the discovery of naturally occurring soluble fragment(s) of human M-CSF (CSF-I) receptor makes possible the recombinant production of such naturally occurring fragments for therapeutic uses where neutralization of M-CSF activity is desired.
• the invention provides a fusion protein comprising the aforementioned polypeptide and a second polypeptide, wherein the aforementioned polypeptide and the second polypeptide are linked such that the normal biological activity of the aforementioned polypeptide is not compromised.
• fusion proteins include immunoglobulin Fc fusions.
• a pharmaceutical composition comprising a specific naturally occurring form of soluble M-CSF receptor or fusion protein, as well as methods of using such pharmaceutical compositions to treat cancer or metabolic bone diseases associated with increased osteoclast activity.
• the invention provides a method of diagnosing cancer comprising the step of: (a) analyzing a fluid sample from a patient for level of soluble M- CSF receptor, wherein a level of soluble M-CSF receptor above a threshold is correlated with the presence of cancer and a level below said threshold indicates that the patient is unlikely to have cancer.
• a method of determining prognosis in a subject afflicted with cancer comprising the step of: (a) analyzing
• a fluid sample from a patient for level of soluble M-CSF receptor wherein a level of soluble M-CSF receptor above a threshold indicates that the patient is likely to have a poor prognosis and a level below said threshold indicates that the patient likely has a good prognosis.
• a method of monitoring cancer therapy in a subject afflicted with cancer comprising the steps of (a) analyzing a fluid sample from a patient for level of
• soluble M-CSF receptor prior to the initiation of treatment with a cancer therapeutic; and (b) analyzing the fluid sample after the initiation of the treatment with the cancer therapeutic, wherein a reduction in the level of soluble M-CSF receptor after the initiation of the treatment with the cancer therapeutic indicates the patient is receiving a therapeutically effective dose of the cancer therapeutic.
• cancers include breast, lung, renal, multiple myeloma, thyroid, prostate, adenocarcinoma, blood cell malignancies, including leukemia and lymphoma; head and neck cancers; gastrointestinal cancers, including esophageal cancer, stomach cancer, colon cancer, '.0 intestinal cancer, colorectal cancer, rectal cancer, pancreatic cancer, liver cancer, cancer of the bile duct or gall bladder; malignancies of the female genital tract, including ovarian carcinoma, uterine endometrial cancers, vaginal cancer, and cervical cancer; bladder cancer; brain cancer, including neuroblastoma; sarcoma, osteosarcoma; and skin cancer, including malignant melanoma or squamous cell cancer.
• a method of monitoring menstrual cycle in a female comprising the step of: (a) analyzing a fluid sample from a female patient for level of soluble M-CSF receptor, wherein a level of soluble M-CSF receptor above a threshold indicates that the patient is likely fertile and a level below the threshold indicates that the patient is likely not fertile.
• JO detecting endometrial proliferation in a female comprising similar steps, wherein a level of soluble M-CSF receptor above a threshold is correlated with abnormal endometrial proliferation, e.g. endometriosis, and a level below the threshold indicates that the patient likely has a normal endometrial proliferative state.
• exemplary fluid samples that may be analyzed according to any of the preceding methods regarding cancer or endometrial proliferation include urine, plasma, or serum.
• the invention also provides a processor, a computer readable memory, and a routine stored on the computer readable memory and adapted to be executed on the processor to perform any of the preceding methods, and/or to generate as output the detected level of soluble M-CSF receptor and a threshold or range of threshold levels considered "normal", such that levels outside the "normal" range correlate with one or more of the conditions described above.
• the invention further provides computer readable media containing programs or routines to perform similar functions.
• suitable computing systems, environments, and/or configurations include personal computers, server computers, hand-held or laptop devices, multiprocessor systems, microprocessor-based systems, set top boxes, programmable consumer electronics, network PCs, minicomputers, mainframe computers, distributed computing environments that include any of the above systems or devices, or any other systems known in the art.
• a kit comprising: (a) a first antibody that specifically binds to soluble M-CSF receptor; and (b) an M-CSF ' receptor standard containing a known quantity of M-CSF receptor.
• the aforementioned kit is provided wherein the first antibody is linked to a detectable label.
• the label is an enzyme.
• the kit further comprises a substrate from which the enzyme releases a detectable signal.
• the aforementioned kit further comprises a second antibody that binds to soluble M-CSF receptor.
• the aforementioned kit further comprises a second antibody that binds to the first antibody.
• the aforementioned kit further comprises a second antibody that binds to soluble M-CSF receptor.
• the soluble M-CSF receptor levels detect the presence of, predict prognosis or severity of, or monitor the progression of a disease selected from the group consisting of osteolytic diseases associated with relatively increased osteoclast activity or the osteoclastic/osteoblastic process required for bone remodeling, including metabolic bone diseases, endocrinopathies (hypercortisolism, hypogonadism, primary or secondary hyperparathyroidism, hyperthyroidism), hypercalcemia, deficiency states (rickets/osteomalacia, scurvy, malnutrition), chronic diseases (malabsorption syndromes, chronic renal failure (renal osteodystrophy), chronic liver disease (hepatic osteodystrophy)), drugs (glucocorticoids (glucocorticoid-induced osteoporosis), heparin, alcohol), and hereditary diseases (osteogenesis imperfecta, homocystinuria), cancer, osteoporosis, periprosthetic bone loss
• osteolytic diseases associated with
• Figure 1 shows the standard curve used to quantify the concentration of soluble human M-CSF receptor.
• Figure 2 shows the detection of soluble human M-CSF receptor in human serum samples.
• Figure 3 shows the binding of human M-CSF by soluble c-fins in human [5 serum.
• Figure 4 shows the affinity analysis of soluble human M-CSF receptor.
• FIG. 5 shows the immunoprecipitation (IP) of soluble human M-CSF receptor.
• Figure 6 shows the deglycosylation of soluble human M-CSF receptor.
• Figure 7 shows the SDS-PAGE analysis of soluble human M-CSF receptor.
• Figure 8 shows the sequence analysis of a peptide (SEQ ID NO: 1) from Trypsin digestion of deglycosylated soluble human M-CSF receptor.
• Figure 9 shows the detection of soluble human M-CSF receptor in human urine samples.
• Figure 10 shows the soluble human M-CSF receptor level in urine samples during menstrual cycle.
• Figure 11 shows the level of soluble human M-CSF receptor in breast cancer patient samples.
• Figure 12 shows the correlation between soluble human M-CSF receptor and M-CSF level in breast cancer patient serum samples.
• Figure 13 shows that soluble M-CSF receptor was found in Cynomulgus and rhesus monkeys.
• Figure 14 shows the expression of soluble human M-CSF receptor during osteoclast differentiation depends on M-CSF activity.
• Figure 15 shows that soluble human M-CSF receptor expression follows TRAP activity of osteoclast.
• Figure 16 shows that removal of M-CSF from culture media stimulates soluble human M-CSF receptor expression of differentiated osteoclasts.
• Figure 17 shows the effect of Zometa and M-CSF neutralizing antibody on the expression of soluble human M-CSF receptor in differentiated osteoclasts.
• the present invention is based on the discovery of a naturally occurring soluble form of human M-CSF receptor, which is normally a membrane-bound receptor. Concentrations of this soluble form of the receptor (shM-CSFR) have been shown to be correlated with cancer, osteoclast differentiation and menstrual cycle. Increasing concentrations of shM-CSFR have been shown to be correlated with severity of cancer and cellular proliferative states.
• the invention provides methods of detecting or monitoring cellular proliferative states, particularly proliferative states found in cancer, endometriosis, and the buildup of uterine lining during a female's fertile period. The methods include methods of diagnosing, staging, predicting prognosis or severity, and monitoring progress or regression of such proliferative states, e.g. during and/or after cancer chemotherapy, endometriosis therapy or during the menstrual cycle.
• the invention also provides methods of detecting or monitoring osteoclast proliferative states, particularly those involving osteoclast differentiation.
• the methods include methods of diagnosing, staging, predicting prognosis or severity, and monitoring progress or regression of metabolic bone diseases during and/or after therapy.
• the invention also provides a purified shM-CSFR.
• the shM-CSFR is purified from human serum or urine and sequenced according to methods known in the art. For example, affinity chromatography with a specific antibody against c- fms is used for affinity purification.
• the antibodies Duo Set Elisa development system
• Any methods of detecting a soluble protein in a patient sample may be used to detect the presence or relative quantity of shM-CSFR associated with any of the above-
• Particularly preferred methods include any antibody-based immunoassays known in the art, such as ELISA (Enzyme-linked immunosorbent assay), RIA. (Radioimmunoassay), LIA (luminescent immunoassay), and FIA (fluorescent immunoassay).
• the antibodies - either primary or secondary - may be labelled with any labels known in the art, including radioisotopes (e.g., 125 I), fluorescent dyes (e.g., FITC) or enzymes (e.g., HRP or
• Steps of such methods include analyzing a fluid sample from a patient for the presence of or relative level of shM-CSF receptor, wherein a level of soluble M-CSF receptor above a threshold indicates that the patient is likely to have a disease state or condition and a level below said threshold indicates that the patient is unlikely to have such disease state or 25 condition.
• Sensitivity and specificity can each be set at any desired level, e.g. 855, 90%, 95%, 96% 97%, 98%, 99% or higher.
• diagnosis means detecting a disease or disorder or determining the stage or degree of a disease or disorder.
• diagnosis also encompasses detecting a predisposition to a disease or disorder, determining the therapeutic effect of a drug therapy, or predicting the pattern of response to a drug therapy.
• the diagnosis methods of the present invention may be used independently, or in combination with other diagnosing and/or staging methods known in the medical art for a particular disease or disorder.
• the method of diagnosis is conducted by detecting, in a patient, the concentrations of shM-CSFR of the present invention using any one of the methods described herein, and determining whether the patient has an aberrant concentration of the protein.
• the diagnostic methods are applied to a subset of patients suspected of having the disease or condition because of other clinical signs or symptoms of disease. "Prognosis" is a forecast as to the probable outcome of an attack or disease, and/or the prospect as to recovery from a disease as indicated by the nature and symptoms of the disease. By way of example, a poor prognosis forecasts an unlikely recovery and possibly death, while a good prognosis forecasts a likely recovery and possibly complete return of health.
• threshold means a quantitative or qualitative measure (e.g., concentration, level, activity, etc.) of a known substance (e.g., a molecule, compound, etc.) under defined conditions that correlates with presence or absence of a condition. In this way, the measure of the known substance under various conditions can be compared to the threshold measure to assist in determining whether a patient is suffering from the condition.
• standard refers to a set range of specific measures (e.g., concentration) of a known substance (e.g., M-CSFR) under defined conditions.
• the term “analyze” means to detect or measure a component in a complex substance (e.g., a fluid such as serum).
• Monitoring is the process of periodically observing or testing the effect of a procedure (e.g., a procedure for treating a patient afflicted with cancer).
• Staging refers to prediction of the clinical stage of a cancer or other disease (e.g. early stage or advanced stage) by analyzing a marker level that has been correlated with clinical stage as determined by accepted medical practice.
• Tumor refers to all neoplastic cell growth and proliferation, whether malignant or benign, and all pre-cancerous and cancerous cells and tissues.
• cancer and “cancerous” refer to or describe the physiological condition in mammals that is typically characterized by unregulated cell growth. Examples of cancer include but are not limited to, carcinoma,; lymphoma, blastoma, sarcoma, and leukemia.
• cancers include breast cancer, prostate cancer, colon cancer, squamous cell cancer, small-cell lung cancer, non-small cell lung cancer, gastrointestinal cancer, pancreatic cancer, glioblastoma, cervical cancer, ovarian cancer, liver cancer, bladder cancer, hepatoma, colorectal cancer, endometrial carcinoma, salivary gland carcinoma, kidney cancer, liver cancer, vulval cancer, thyroid cancer, hepatic carcinoma and various types of head and neck cancer.
• Treatment is an intervention performed with the intention of preventing the development or altering the pathology of a disorder. Accordingly, “treatment” refers to both therapeutic treatment and prophylactic or preventative measures. Those in need of treatment include those already with the disorder as well as those in which the disorder is to be prevented.
• a therapeutic agent may directly decrease the pathology of tumor cells, or render the tumor cells more susceptible to treatment by other therapeutic agents, e.g., radiation and/or chemotherapy.
• Treatment of patients suffering from clinical, biochemical, radiological or subjective symptoms of the disease, such as osteolysis may include alleviating some or all of such symptoms or reducing the predisposition to the disease.
• the "pathology" of cancer includes all phenomena that compromise the well being of the patient.
• mammal for purposes of treatment refers to any animal classified as a mammal, including humans, domestic and farm animals, and zoo, sports, or pet animals, such as dogs, horses, cats, cows, etc. Preferably, the mammal is human.
• metastatic cancer is defined as cancers that have potential to spread to other areas of the body, particularly bone.
• a variety of cancers can metastasize to the bone, but the most common metastasizing cancers are breast, lung, renal, multiple myeloma, thyroid and prostate.
• cancers that have the potential to metastasize to bone include but are not limited to adenocarcinoma, blood cell malignancies, including leukemia and lymphoma; head and neck cancers; gastrointestinal cancers, including esophageal cancer, stomach cancer, colon cancer, intestinal cancer, colorectal cancer, rectal cancer, pancreatic cancer, liver cancer, cancer of the bile duct or gall bladder; malignancies of the female genital tract, including ovarian carcinoma, uterine endometrial cancers, vaginal cancer, and cervical cancer; bladder cancer; brain cancer, including neuroblastoma; sarcoma, osteosarcoma; and skin cancer, including malignant melanoma and squamous cell cancer.
• the present invention especially contemplates prevention and treatment of tumor-induced osteolytic lesions in bone.
• the phrase "therapeutically effective amount” refers to is meant to refer to an amount of therapeutic or prophylactic soluble fragment of the M-CSF receptor that would be appropriate for an embodiment of the present invention, that will elicit the desired therapeutic or prophylactic effect or response when administered in accordance with the desired treatment regimen.
• M-CSF refers to a human polypeptide having substantially the same amino acid sequence as the mature human M-CSFa, M-CSF ⁇ , or M- CSF ⁇ polypeptides described in Kawasaki et al., Science 230:291 (1985), Cerretti et al., Molecular Immunology, 25:761 (1988), or Ladner et al., EMBO Journal 6:2693 (1987), each of which are incorporated herein by reference.
• M-CSF dimer refers to two M-CSF polypeptide monomers that have dimerized and includes both homodimers
• M-CSF monomers may be converted to M-CSF dimers in vitro as described in U.S. Pat. No. 4,929,700, which is incorporated herein by reference.
• Soluble human M-CSF receptor means a polypeptide having substantially the same amino acid sequence as the extracellular portion of M-CSFR of SEQ ID NO: 2.
• Monoclonal or polyclonal antibodies may be used in the detection methods of the present invention, although monoclonal antibodies are advantageously used because of their consistency and specificity.
• Rodent antibodies such as murine monoclonal antibodies are suitable for use in diagnostic methods.
• the modifier "monoclonal” indicates the character of the antibody as being obtained from a substantially homogeneous population of antibodies, and is not to be construed as requiring production of the antibody by any particular method.
• the antigen to be used for production of antibodies may be, e.g., intact shM- CSFR or a fragment of shM-CSFR that retains the desired epitope, optionally fused to another polypeptide that allows the epitope to be displayed in its native conformation.
• cells expressing shM-CSFR can be used to generate antibodies. Such cells can be transformed to express M-CSF or may be other naturally occurring cells that express shM- CSFR. Other forms of shM-CSFR useful for generating antibodies will be apparent to those skilled in the art.
• the nucleic acid encoding it is 0 isolated and inserted into a replicable vector for further cloning (amplification of the DNA) or for expression as is well known in the art.
• DNA encoding the monoclonal antibody is readily isolated and sequenced using conventional procedures (e.g., by using oligonucleotide probes that are capable of binding specifically to genes encoding the heavy and light chains of the antibody).
• Many vectors are available.
• the vector components generally include, but are not L 5 limited to, one or more of the following: a signal sequence, an origin of replication, one or more selective marker genes, an enhancer element, a promoter, and a transcription . termination sequence.
• the antigen is used to generate monoclonal antibodies using any methods known in the art, e.g. the hybridoma method first described by Kohler et al., Nature, 256:495 >0 [19751, or recombinant DNA methods (see, e.g., U.S. Patent No. 4,816,567), or isolated from phage antibody libraries using the techniques described in Clackson et al., Nature, 352:624628[1991] and Marks et al., J. MoI. Biol., 222:581-597 (1991).
• a mouse or other appropriate host animal such as a hamster or macaque monkey
• lymphocytes that 25 produce or are capable of producing antibodies that will specifically bind to the protein used for immunization.
• lymphocytes maybe immunized in vitro. Lymphocytes then are fused with myeloma cells using a suitable fusing agent, such as polyethylene glycol, to form a hybridoma cell (Goding, Monoclonal Antibodies: Principles and Practice, pp.59- 103 (Academic Press, 1986)).
• the hybridoma cells thus prepared are seeded and grown in a suitable culture medium that preferably contains one or more substances that inhibit the growth or survival of the unfused, parental myeloma cells.
• a suitable culture medium that preferably contains one or more substances that inhibit the growth or survival of the unfused, parental myeloma cells.
• the culture medium for the hybridomas typically will include hypoxanthine, aminopterin, and thymidine (HAT medium), which substances prevent the growth of HGPRT-deficient cells.
• Preferred myeloma cells are those that fuse efficiently, support stable high- level production of antibody by the selected antibody-producing cells, and are sensitive to a medium.
• Human myeloma and mouse-human heteromyeloma cell lines also have been described for the production of human monoclonal antibodies (Kozbor, J. Immunol., 133: 3001 (1984) ;Brodeur et al., Monoclonal Antibody Production Techniques and Applications, pp. 51-63 (Marcel Dekker, Inc., New York, 1987)).
• Exemplary murine myeloma lines include those derived from MOP-21 and M.C.-l 1 mouse tumors available from the SaIk Institute Cell Distribution Center, San Diego, Calif. USA, and SP-2 or X63-Ag8-653 cells available from the American Type Culture Collection, Rockville, Md. USA.
• Culture medium in which hybridoma cells are growing is assayed for production of monoclonal antibodies directed against the antigen.
• the binding specificity of monoclonal antibodies produced by hybridoma cells is determined by immunoprecipitation or by an in vitro binding assay, such as radioimmunoassay (RIA) or enzyme-linked immunoabsorbent assay (ELISA).
• RIA radioimmunoassay
• ELISA enzyme-linked immunoabsorbent assay
• the binding affinity of the monoclonal antibody can, for example, be determined by Scatchard analysis (Munson et al., Anal. Biochem., 107:220 (1980)).
• the clones may be subcloned by limiting dilution procedures and grown by standard methods (Goding, Monoclonal Antibodies: Principles and Practice, pp.59-103 (Academic Press, 1986)). Suitable culture media for this purpose include, for example, D-MEM or RPMI-1640 medium.
• the hybridoma cells may be grown in vivo as ascites tumors in an animal.
• the monoclonal antibodies secreted by the subclones are suitably separated from the culture medium, ascites fluid, or serum by conventional immunoglobulin purification procedures such as, for example, protein A- Sepharose, hydroxylapatite chromatography, gel electrophoresis, dialysis, or affinity chromatography.
• antibody is used in the broadest sense and includes fully assembled antibodies, monoclonal antibodies, polyclonal antibodies, multispecific antibodies (e.g., bispecific antibodies), antibody fragments that can bind antigen (e.g., Fab', F'(ab)2, Fv, single chain antibodies, diabodies), chimeric antibodies, humanized or human-derived antibodies, and recombinant peptides comprising the forgoing as long as they exhibit the desired biological activity.
• antigen e.g., Fab', F'(ab)2, Fv, single chain antibodies, diabodies
• chimeric antibodies e.g., humanized or human-derived antibodies, and recombinant peptides comprising the forgoing as long as they exhibit the desired biological activity.
• chimeric or humanized antibodies are less immunogenic in humans than the parental mouse monoclonal antibodies, they can be used for the treatment of humans with far less risk of anaphylaxis. Thus, these antibodies may be preferred in therapeutic applications that involve in vivo administration to a human.
• Chimeric monoclonal antibodies in which the variable Ig domains of a mouse monoclonal antibody are fused to human constant Ig domains, can be generated using standard procedures known in the art (See Morrison, S. L., et al. (1984) Chimeric Human Antibody Molecules; Mouse Antigen Binding Domains with Human Constant Region Domains, Proc. Natl. Acad. Sci. USA 81, 6841-6855; and, Boulianne, G. L., et al, Nature 312, 643-646. (1984)). Although some chimeric monoclonal antibodies have proved less immunogenic in humans, the mouse variable Ig domains can still lead to a significant human anti-mouse response.
• Humanized antibodies may be achieved by a variety of methods including, for example: (1) grafting the non-human complementarity determining regions (CDRs) onto a human framework and constant region (a process referred to in the art as humanizing through “CDR grafting"), or, alternatively, (2) transplanting the entire non-human variable domains, but “cloaking" them with a human-like surface by replacement of surface residues (a process referred to in the art as “veneering”).
• CDRs complementarity determining regions
• the invention provides a method for detecting shM-CSFR in a biological sample comprising contacting the sample with an antibody that specifically binds to shM- CSFR and detecting either the antibody bound to shM-CSFR or the unbound antibody.
• the relative amounts of either correlate to the amount of shM-CSFR in the sample.
• the antibody is directly or indirectly labeled with a detectable label.
• Suitable detectable labels include various enzymes, binding groups, fluorescent materials, luminescent materials and radioactive materials.
• Exemplary enzyme labels include horseradish peroxidase, alkaline phosphatase, beta-galactosidase, or acetylcholinesterase;
• exemplary binding group complexes include streptavidin/biotin and avidin/biotin;
• exemplary fluorescent labels include umbel liferone, fluorescein, fluorescein isothiocyanate, rhodamine, dichlorotriazinylamine fluorescein, dansyl chloride or phycoerythrin;
• an exemplary luminescent label includes luminol; and exemplary radioactive labels include 125 1, 131 1, 35 S or 3 H.
• shM-CSFR can be detected by a competition immunoassay utilizing shM-CSFR standards linked to a detectable label and an unlabeled anti-shM-CSFR antibody.
• the biological sample, the labeled shM-CSFR standards and the anti-shM-CSFR antibody are combined and the amount of labeled anti-shM-CSFR standard bound to the unlabeled antibody is determined.
• the amount of shM-CSFR in the biological sample is inversely proportional to the amount of labeled shM-CSFR standard bound to the anti-shM-CSFR antibody.
• An ELISA assay initially comprises preparing an antibody, specific to shM- CSFR, preferably a monoclonal antibody.
• a reporter antibody generally is prepared which binds specifically to shM-CSFR.
• the reporter antibody is attached to a detectable reagent such as radioactive, fluorescent or enzymatic reagent, for example horseradish peroxidase enzyme (HRP) or alkaline phosphatase (AP).
• HRP horseradish peroxidase enzyme
• AP alkaline phosphatase
• antibody specific to shM-CSFR is incubated on a solid support, e.g. a polystyrene dish, that binds the antibody. Any free protein binding sites on the dish are then covered by incubating with a non-specific protein such as bovine serum albumin (BSA).
• BSA bovine serum albumin
• the sample to be analyzed is incubated in the dish, during which time shM-CSFR binds to the specific antibody attached to the polystyrene dish. Unbound sample is washed out with buffer. A reporter antibody specifically directed to shM-CSFR and linked to horseradish peroxidase is placed in the dish resulting in binding of the reporter antibody to any monoclonal antibody bound to shM-CSFR. Unattached reporter antibody is then washed out. Reagents for peroxidase activity, including a colorimetric substrate, are then added to the dish. Immobilized peroxidase, linked to anti-shM-CSFR antibodies, generate a colored reaction product. The amount of color developed in a given time period is proportional to the amount of shM-CSFR protein present in the sample. Quantitative results typically are obtained by reference to a standard curve.
• the assay device is a lateral flow test strip, optionally encased in a housing.
• a first labeled antibody to shM-CSFR is in solution, while a second antibody to shM-CSFR is immobilized on the test strip.
• an antibody-target-antibody sandwich complex is formed, and the resulting complex, which is immobilized on the solid support, is detectable by virtue of the label.
• the test strip is then inserted into a reader, where the signal from the label in the complex is measured.
• the outcome may be either a positive or negative result, or a quantitative determination of the concentration of shM-CSFR in the sample, which is correlated with a result indicative of a risk or presence of a disease or disorder.
• the entire procedure may be automated and/or computer-controlled.
• the test strip may be read visually by comparison to a visual standard of the appropriate color. This test provides similar clinically relevant information as a shM-CSFR ELISA, but in significantly less time and at the point of care.
• the device is a test strip for use in the rapid detection of shM-CSFR, using immunoassay methods in which shM-CSFR in a fluid sample compete with immobilized shM-CSFR for limited labeled antibody binding sites.
• the body fluid sample mixes with labeled antibody-dye conjugate and migrates along a porous membrane.
• concentration of shM-CSFR is below the detection limit of the test, unbound antibody-dye conjugate binds to shM-CSFR conjugate immobilized on the membrane, producing a color band in the "negative" test zone.
• a threshold by which disease states, prognoses and the like are measured are well known in the art.
• levels of shM-CSFR protein in a fluid sample from a sufficient representative number of normal subjects e.g. healthy population without the condition to be detected
• shM-CSFR protein level from a sufficient representative number of diseased subjects e.g. population confirmed to have the disease or condition.
• a threshold cutoff can be determined that differentiates most of the normal population from most the diseased population.
• useful end point values for negative, uncertain and positive results can be determined from the data.
• a normal range (indicative of a negative result) can be determined, which includes shM-CSFR levels of most of the normal population but which exclude almost all of the diseased population.
• a range indicative of a positive result can be determined, which includes shM-CSFR levels of most of the diseased population but which exclude almost all of the normal population.
• Measured levels of shM-CSFR protein either above (or below, as appropriate) the predetermined threshold are indicative of an association of shM-CSFR protein with the disease.
• samples from the normal population should be subjected to identical protocols (i.e., preparation and storage) as the samples from the unhealthy population.
• thresholds take account of overall medical and epidemiological factors. Factors to be considered include the clinical objective of the laboratory test and whether it is necessary to have a high positive predictive value, or a high negative predictive value, as well as prevalence of the disease in the test population.
• Kits are also contemplated within the scope of the invention.
• a typical kit can comprise a first antibody that specifically binds to shM-CSFR, optionally linked to a detectable label, and an M-CSFR standard containing a known quantity of M-CSFR.
• Other components may optionally include reagents for carrying out an immunoassay such as a second antibody linked to a detectable label that either binds to shM-CSFR or to the first antibody; if the label is an enzyme, the kit may also include a substrate from which the enzyme releases a detectable signal.
• compositions comprising one or more M-CSF antagonists may be administered to persons or mammals suffering from, or predisposed to suffer from, cancer metastasis and/or bone loss associated with cancer metastasis.
• Concurrent administration of two therapeutic agents does not require that the agents be administered at the same time or by the same route, as long as there is an overlap in the time period during which the agents are exerting their therapeutic effect.
• Simultaneous or sequential administration is contemplated, as is administration on different days or weeks.
• Combining the shM-CSFR therapy method with a chemotherapeutic or radiation regimen may also be carried out.
• Administration and preparation may be carried out.
• the shM-CSFR used in the practice of a method of the invention maybe formulated into a pharmaceutical composition comprising a carrier suitable for the desired delivery method.
• Suitable carriers include any material which, when combined with the shM-CSFR. retains the anti-tumor function of the shM-CSFR and is non-reactive with the subject's immune systems. Examples include, but are not limited to, any of a number of standard pharmaceutical carriers such as sterile phosphate buffered saline solutions, bacteriostatic water, and the like.
• aqueous carriers may be used, e.g., water, buffered water, 0.4% saline, 0.3% glycine and the like, and may include other proteins for enhanced stability, such as albumin, lipoprotein, globulin, etc., subjected to mild chemical modifications or the like.
• Therapeutic formulations of the drug are prepared for storage by mixing the drug having the desired degree of purity with optional physiologically acceptable carriers, excipients or stabilizers (Remington's Pharmaceutical Sciences 16th edition, Osol, A. Ed. (1980)), in the form of lyophilized formulations or aqueous solutions.
• Acceptable carriers, excipients, or stabilizers are nontoxic to recipients at the dosages and concentrations employed, and include buffers such as phosphate, citrate, and other organic acids; antioxidants including ascorbic acid and methionine; preservatives (such as octadecyldimethylbenzyl ammonium chloride; hexamethonium chloride; benzalkonium chloride, benzethonium chloride; phenol, butyl or benzyl alcohol; alkyl parabens such as methyl or propyl paraben; catechol; resorcinol; cyclohexanol; 3-pentanol; and m-cresol); low molecular weight (less than about 10 residues) polypeptides; proteins, such as serum albumin,
• hydrophilic polymers such as polyvinylpyrrolidone
• amino acids such as glycine, glutar ⁇ ine, asparagine, histidine, arginine, or lysine
• chelating agents such as EDTA
• sugars such as sucrose, mannitol, trehalose or sorbitol
• salt-forming counter-ions such as sodium
• metal complexes e.g., Zn-protein complexes
• surfactants such as TWEENTM, PLURONICSTM or polyethylene glycol (PEG).
• compositions of the invention are administered to a mammal in an amount sufficient to prevent or at least partially arrest the progression of cancer, cancer metastasis and/or osteolysis.
• An amount adequate to accomplish this is defined as a "therapeutically effective dose.”
• Single or multiple administrations of the compositions can be carried out 5 with the dose levels and pattern being selected by the treating physician.
• the appropriate dosage will depend on the type of disease to be treated, as defined above, the severity and course of the disease, whether drug is administered for preventive or therapeutic purposes, previous therapy, the patient's clinical history and response to the drug, and the discretion of the attending physician.
• the shM-CSFR of the invention may also be used as affinity purification agent for M-CSF or in diagnostic assays for M-CSF protein, e.g., detecting its expression in specific cells, tissues, or serum.
• the shM-CSFR may also be used for in vivo diagnostic assays.
• the shM-CSFR is labeled with a radionuclide (such as >5 l ' 1 In, 99 Tc, 14 C, 131 I, 125 I, 3 H, 32 P or 35 S) so that the tumor can be localized using immunoscintiography.
• the shM-CSFR of the present invention may be employed in any known assay method, such as competitive binding assays, direct and indirect sandwich assays, such as ELISAs, and immunoprecipitation assays. Zola, Monoclonal Antibodies: A Manual of 50 Techniques, pp.147-158 (CRC Press, Inc. 1987).
• the shM-CSFR of the present invention when used to detect M-CSF can be provided in a kit, i.e., a packaged combination of reagents in predetermined amounts with instructions for performing the diagnostic assay, optionally including an M-CSF standard containing a known quantity of M-CSF.
• a kit i.e., a packaged combination of reagents in predetermined amounts with instructions for performing the diagnostic assay, optionally including an M-CSF standard containing a known quantity of M-CSF.
• other additives may be included such as stabilizers, buffers (e.g., a block buffer or lysis buffer) and the like.
• the relative amounts of the various reagents may be varied widely to provide for concentrations in solution of the reagents which substantially optimize the sensitivity of the assay.
• the reagents may be provided as dry powders, usually lyophilized, including excipients which on dissolution will provide a reagent solution having the appropriate concentration.
• This example describes the identification of a soluble receptor for human M- CSF and shows that this soluble receptor is capable of binding M-CSF.
• Micro titer plates (R&D systems, Cat# CPOOl 1) were coated with 100 ⁇ l/well capture antibody (Duo Set Elisa development system hM-CSF R ( R&D systems, Cat# DY329)) at a working dilution of 100 ng/ml in PBS. The plates were sealed and incubated at room temperature overnight. After washing the wells 3 times with wash buffer (0.05% Tween in PBS, pH 7.2-7.4) with a manifold dispenser/washer, plates were blocked with 300 ⁇ l/well of blocking buffer (1% BSA, 5% Sucrose in PBS, pH 7.2-7 '.4) at room temperature for at least 1 hour. After repeating the wash step, the plates were ready for sample addition.
• wash buffer 0.05% Tween in PBS, pH 7.2-7.4
• Streptadvidin HRP Duo Set Elisa development system hM-CSFR (R&D systems, Cat# DY329)
• Streptadvidin HRP Duo Set Elisa development system hM-CSFR (R&D systems, Cat# DY329)
• the plates were developed for 5 minutes with 100 ⁇ l substrate solution (1:1 Mixture of Reagent A and B (R&D systems, Cat# DY999)) per well, followed by addition of 50 ⁇ l stop solution (2N H 2 SO 4 ), and gentle tapping of the plate to ensure thorough mixing.
• the final readout was the optical density at 450-540 nm immediately after addition of stop solution.
• a strong positive signal was detected in human serum samples when the ELISA assay was used to determine the presence of soluble human M-CSF receptor in serum samples. Both the capture antibody and detection antibody used in this ELISA assay were specific for human M-CSF receptor extra-cellular domain. As shown in Figure 2, a strong positive signal was detected in human serum samples. The signal is dependent on the concentration of human serum. There is no signal when no capture antibody was used.
• Micro titer plates (R&D systems, Cat# CPOOIl) were coated with 100 ⁇ l /well capture antibody (Duo Set Elisa development system hM-CSF R ( R&D systems, Cat# DY329)) at a working dilution of 100 ng/ml in PBS. The plates were sealed and incubated at room temperature overnight. After washing the wells 3 times with wash buffer (0.05% Tween in PBS, pH 7.2-7.4) with manifold dispenser/washer, plates were blocked with 300 ⁇ l/well blocking buffer (1% BSA, 5% Sucrose in PBS, pH 7.2-7.4) at room temperature for at least 1 hour.
• wash buffer 0.05% Tween in PBS, pH 7.2-7.4
• manifold dispenser/washer plates were blocked with 300 ⁇ l/well blocking buffer (1% BSA, 5% Sucrose in PBS, pH 7.2-7.4) at room temperature for at least 1 hour.
• a serial dilution of human serum sample was applied to an ELISA plate coated with a mouse monoclonal anti-human c-fms antibody.
• Either exogenous human M-CSF at 1 ⁇ g/ml (squares) or PBS. (diamonds) was added before detection of human M- CSF with an alkaline phosphatase-conjugated polyclonal anti-human M-CSF antibody. Distinct binding of M-CSF was found above 5% human serum.
• the soluble receptor in human serum was found to have lower binding affinity as compared with recombinant c-fms Fc fusion protein ( Figure 4) [The binding affinity of the recombinant c- fms Fc fusion protein is considered to be close to that of the native membrane-bound c-fms because of the ligand-induced dimerization].
• One interpretation for the low affinity of the soluble receptor is that it has only single binding site for the ligand, while the recombinant c- frns Fc fusion protein has two binding sites for avidity effect.
• the sample was spun for 5 minutes at 400 rpm and the supernatant was transferred to a fresh tube, and the supernatant was saved at the same time.
• the beads were transferred to an Eppendorf tube and washed once with 1 ml each of wash buffers #1(0.5 M LiCL 2 ), #2(0.5 M LiCL 2 / 0.5% Triton) and #3(10 tnM TRIS pH 7.4). After the last spin, most of the wash buffer was removed (until the beads were almost dry). The beads were then re-suspended in 500 ⁇ l PBS. Before running the gel (10% Novex), 5x SDS sample buffer with DTT was added, and the 5 samples were boiled for 2 minutes at 100 0 C.
• frozen beads from the above immunoprecipitation procedure ( ⁇ 200 ⁇ l) were diluted with 200 ⁇ l PBS at a 1 :1 ratio.
• the diluted sample was incubated in Ix denaturation buffer (PNGase F, NEB# P0705S) at 100 0 C for 10 minutes.
• G7 reaction buffer i 0 PNGase F, NEB # P0705S
• Ix final concentration NP40
• the soluble human M-CSF receptor was found to be a glycosylated protein with a molecular weight around 97 KD ( Figure 5). After deglycosylation, the molecular 15 weight of the soluble human M-CSF receptor is about 60 KD ( Figure 6).
• the soluble human M-CSF receptor was found to share, at least partially, the protein sequence of the human c-fins protein, which is the membrane-bound receptor for human M-CSF. As shown in above figures, the soluble human M-CSF receptor can be recognized by multiple antibodies that also recognizes human c-fins protein. To further 0 confirm the protein sequence of the soluble human M-CSF receptor, a protein analysis was performed as follows: A protein band was identified on a SDS-PAGE gel (band #4 in Figure 7) that corresponded to the soluble receptor after deglycosylation. The protein in this band was digested with trypsin. The product of this digestion, a mixture of peptides, was analyzed.
• EXAMPLE 2 This Example shows that the soluble M-CSF receptor was found in human urine samples. This Example further shows that this receptor is present in serum from both normal subjects and breast cancer patients, and the soluble M-CSF receptor level correlated with the M-CSF level. Finally, this Example shows that the soluble M-CSF receptor is also found in primates.
• Soluble human M-CSF receptor was also found in urine samples Using the same ELISA setup as described in Example 1, multiple urine samples from healthy human volunteers were collected and assayed for the presence of soluble human M-CSF receptor. Varying levels of soluble human M-CSF receptor were detected among adult female (age from 25 to 66), adult male (age from 35 to 70) and children from age 3 to 9. Quantification of the concentration of soluble human M-CSF receptor was determined with recombinant c-fms-human Fc fusion protein as reference ( Figure 9).
• Urine samples were collected daily throughout the menstrual cycle of one female subject. The samples were collected as first void in the morning and frozen at — 20 0 C. All samples were assayed on the same date. Soluble receptor was assayed as described herein. As shown in Figure 10, soluble receptor level urine samples fluctuates during the menstrual cycle. This finding makes the soluble receptor a potential diagnostic marker for the menstrual cycle, or for use in pregnancy tests.
• Soluble human M-CSF receptor concentrations were analyzed in breast cancer serum samples as follows. A serial dilution of human serum sample was assayed in an ELISA for the presence of soluble human M-CSF receptor as described above. A strong positive signal was detected when the human serum was assayed in this ELISA format (referred to as "regular"). As controls, removal of either the capture antibody or detection antibody eliminated the signal, indicating that the signal is specific for these anti-human c- fms antibodies. Quantification of the concentration of soluble human M-CSF receptor was determined to be 0.5 ug/ml, with recombinant c-fms-human Fc fusion protein as reference.
• the level of soluble receptor varies among patients. It is noted that the soluble receptor concentrations in the patients' samples are much lower than described above ( Figure 2). likely due to different protocols used for serum sample preparation and storage. Consequently, the comparison of M-CSF and M-CSF soluble receptor concentration is valid only among samples collected and treated according to the same protocol.
• Figure 12 shows a linear correlation between M-CSF levels in the breast cancer patients versus M-CSF soluble receptor concentration in the same sample. This finding makes M-CSF soluble receptor a potential biomarker for cancer progression. Moreover, M-CSF soluble receptor becomes a more useful biomarker when direct measurement of M-CSF becomes difficult (e.g., when a M-CSF neutralizing antibody is used as a treatment of cancer). *
• the ELISA assay described above for human M-CSF soluble receptor was also used to test serum samples from other animal species ( Figure 13). Cynomulgus monkey and Rhesus monkey serum samples show a strong positive signal, indicating the presence of soluble receptors.
• the protein sequences of the membrane-bound M-CSF receptor for Cyno and Rhesus monkeys are not available. However, it is believed that they share a high level of protein homology with their human counterpart. Therefore, the antibodies used in the ELISA assay, which recognize the extracellular domain of human membrane-bound M-CSF receptor, also recognize that of the Cyno and Rhesus monkeys. It should be noted that the lack of positive signal from non-primate animal serum does not indicate the lack of M-CSF soluble receptor. Rather, it is most likely due to the antibody specificity of the ELISA assay used to detect the presence of soluble receptor.
• EXAMPLE 3 This Example shows that the expression of soluble human M-CSF receptor is tightly coupled with osteoclast differentiation. This Example further shows that only differentiated osteoclasts express the soluble human M-CSF receptor, the level of which increases upon removal of M-CSF.
• M-CSF soluble receptor was studied in an in vitro system with human osteoclast celts, where the expression of the membrane-bound M-CSF receptor was found. In particular, the expression of the soluble receptor was examined in the context of osteoclast differentiation.
• Primary human Osteoclast Precursors (Cambrex Bio Science Walkersville, Inc. Cat# 2T- 110) were seeded at 10,000 cells/well at 0.2 ml/well in the cell culture media containing 30 ng/ml human M-CSF and 100 ng/ml RANKL. Test antibodies were added to the wells at 1 ⁇ g/mL on the same day of cell plating.
• the cells were incubated at 37 0 C, in a humidified atmosphere of 5% CO 2 .
• osteoclasts were identified by phase microscopy as unusually large multinucleate cells.
• the culture can be continued for an additional week, with feeding, during which time the osteoclasts will continue to increase in size.
• cells were stained for tartrate-resistant acid phosphatase, the 5 positive staining of which indicates the presence of osteoclasts.
• the number of osteoclasts in each well was counted under the light microscope and shown in the graph as an average of osteoclasts under the viewing field of the microscope.
• BoneTRAP® Assay is a solid-phase 0 immuno fixed enzyme activity assay for the rapid and specific determination bf bone resorption rate from human serum samples. It can be used in human osteoclast cultures.
• the M-CSF soluble receptor was expressed in osteoclast cells.
• the concentration of the soluble receptor in the conditioned media of L 5 . differentiated osteoclasts was measured.
• the expression of soluble receptor was specific to osteoclasts. Inhibition of osteoclast differentiation by a M-CSF neutralizing antibody, Chir- RXl in this case, resulted in the suppression of soluble receptor.
• Soluble receptor is expressed only in differentiated osteoclasts, not osteoclast precursors. As shown by a time course experiment shown in Figure 15a, soluble receptor .0 expression starts on Day 10 in the osteoclast differentiation assay and reaches plateau on Day 12. This expression pattern parallels exactly that of osteoclast differentiation, as followed by TRAP activity and shown in Figure 15b.
• M-CSF soluble receptor expression is up-regulated by the removal of M-CSF in differentiated osteoclasts.
• Figure 16 shows the expression of M-CSF 5 soluble receptor within 24 hours after the addition of either an IgGl control antibody or M- CSF neutralizing antibody, Chir-RXl .
• the data clearly show that soluble receptor expression goes up when M-CSF activity is neutralized. The increase of expression starts at Day 8 and reaches the highest level on Day 12, which indicates that the increased expression happens in differentiated osteoclasts.
• Zometa an inhibitor of osteoclast activity, has no effect on the expression of

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