WO2007117852A3 - Targeted deletions using retroelement-mediated placement of recombination sites - Google Patents

Targeted deletions using retroelement-mediated placement of recombination sites Download PDF

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Publication number
WO2007117852A3
WO2007117852A3 PCT/US2007/064031 US2007064031W WO2007117852A3 WO 2007117852 A3 WO2007117852 A3 WO 2007117852A3 US 2007064031 W US2007064031 W US 2007064031W WO 2007117852 A3 WO2007117852 A3 WO 2007117852A3
Authority
WO
WIPO (PCT)
Prior art keywords
recombination sites
retroelement
mediated
placement
nucleic acid
Prior art date
Application number
PCT/US2007/064031
Other languages
French (fr)
Other versions
WO2007117852A2 (en
Inventor
Melissa Spears
Greg Davis
Kevin Kayser
Original Assignee
Sigma Aldrich Co
Melissa Spears
Greg Davis
Kevin Kayser
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sigma Aldrich Co, Melissa Spears, Greg Davis, Kevin Kayser filed Critical Sigma Aldrich Co
Publication of WO2007117852A2 publication Critical patent/WO2007117852A2/en
Publication of WO2007117852A3 publication Critical patent/WO2007117852A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

The present invention provides methods, nucleic acid constructs, and kits for selectively deleting a region of a nucleic acid sequence. Specifically, it utilizes retargeting retroelements to place site-specific recombination sites at targeted locations in the nucleic acid sequence. The region between the recombination sites is then deleted using a site-specific recombination system.
PCT/US2007/064031 2006-03-29 2007-03-15 Targeted deletions using retroelement-mediated placement of recombination sites WO2007117852A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US11/391,972 2006-03-29
US11/391,972 US7919322B2 (en) 2006-03-29 2006-03-29 Targeted deletions using retroelement-mediated placement of recombination sites

Publications (2)

Publication Number Publication Date
WO2007117852A2 WO2007117852A2 (en) 2007-10-18
WO2007117852A3 true WO2007117852A3 (en) 2007-12-06

Family

ID=38559595

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2007/064031 WO2007117852A2 (en) 2006-03-29 2007-03-15 Targeted deletions using retroelement-mediated placement of recombination sites

Country Status (2)

Country Link
US (1) US7919322B2 (en)
WO (1) WO2007117852A2 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0612301D0 (en) * 2006-06-21 2006-08-02 Morvus Technology Ltd DNA molecules and methods
WO2013134499A1 (en) * 2012-03-07 2013-09-12 The Johns Hopkins University Anastasis biosensor
EP3542162B1 (en) 2016-11-16 2021-09-01 The Johns Hopkins University Anastasis biosensor
US11649509B2 (en) 2017-11-16 2023-05-16 The Johns Hopkins University Anastasis biosensor caspase tracker

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4959317A (en) * 1985-10-07 1990-09-25 E. I. Du Pont De Nemours And Company Site-specific recombination of DNA in eukaryotic cells

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5872241A (en) 1995-01-25 1999-02-16 The Trustees Of Columbia University In The City Of New York Multiple component RNA catalysts and uses thereof
US6027895A (en) 1995-09-12 2000-02-22 The Ohio State University Research Foundation Methods for cleaving DNA with nucleotide integrases
US5698421A (en) 1995-09-12 1997-12-16 The Ohio State Research Foundation Ribonucleoprotein particles for cleaving double-stranded DNA and inserting an RNA/DNA molecule into the cleavage site
US5804418A (en) 1996-11-19 1998-09-08 The Ohio State University Research Foundation Methods for preparing nucleotide integrases
US6140129A (en) 1997-09-17 2000-10-31 Wisconsin Alumni Research Foundation Chromosomal targeting in bacteria using FLP recombinase
US6306596B1 (en) 1998-02-26 2001-10-23 The Ohio State University Research Foundation Methods for cleaving single-stranded and double-stranded DNA substrates with nucleotide integrase
KR100505797B1 (en) * 2002-10-11 2005-08-04 씨제이 주식회사 A Mutated Microorganism Having A Knockout fadR Gene On Chromosome And A Process For Producing L-Threonine Using Said Mutant

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4959317A (en) * 1985-10-07 1990-09-25 E. I. Du Pont De Nemours And Company Site-specific recombination of DNA in eukaryotic cells

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
ZHONG ET AL.: "Targeted and random bacterial gene disruption using a group Ii intron (targetron) vector containing a retrotransposition-activated selectable marker", NUCLEIC ACIDS RES., vol. 31, no. 6, 2003, pages 1656 - 1664, XP002456213 *

Also Published As

Publication number Publication date
US7919322B2 (en) 2011-04-05
WO2007117852A2 (en) 2007-10-18
US20070231865A1 (en) 2007-10-04

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