WO2007117331A3 - Novel dna polymerase from thermoanaerobacter tengcongenesis - Google Patents

Novel dna polymerase from thermoanaerobacter tengcongenesis Download PDF

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Publication number
WO2007117331A3
WO2007117331A3 PCT/US2006/062527 US2006062527W WO2007117331A3 WO 2007117331 A3 WO2007117331 A3 WO 2007117331A3 US 2006062527 W US2006062527 W US 2006062527W WO 2007117331 A3 WO2007117331 A3 WO 2007117331A3
Authority
WO
WIPO (PCT)
Prior art keywords
tengcongenesis
thermoanaerobacter
dna polymerase
novel dna
polymerase
Prior art date
Application number
PCT/US2006/062527
Other languages
French (fr)
Other versions
WO2007117331A2 (en
Inventor
Shanmuuga Sundaram
Scott Hamilton
Carl W Fuller
Original Assignee
Ge Healthcare Bio Sciences
Shanmuuga Sundaram
Scott Hamilton
Carl W Fuller
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ge Healthcare Bio Sciences, Shanmuuga Sundaram, Scott Hamilton, Carl W Fuller filed Critical Ge Healthcare Bio Sciences
Publication of WO2007117331A2 publication Critical patent/WO2007117331A2/en
Publication of WO2007117331A3 publication Critical patent/WO2007117331A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1276—RNA-directed DNA polymerase (2.7.7.49), i.e. reverse transcriptase or telomerase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase

Abstract

Disclosed is a DNA polymerase from Thermoanaerobacter tengcongenesis. This enzyme is useful for procedures requiring strand-displacing DNA synthesis such as SDA and for reverse transcription. Included within the scope of the present invention are various mutants (deletion and substitution) that retain the ability to replicate DNA with substantially the same efficiency as the native Thermoanaerobacter tengcongenesis polymerase.
PCT/US2006/062527 2005-12-22 2006-12-22 Novel dna polymerase from thermoanaerobacter tengcongenesis WO2007117331A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US75307505P 2005-12-22 2005-12-22
US60/753,075 2005-12-22

Publications (2)

Publication Number Publication Date
WO2007117331A2 WO2007117331A2 (en) 2007-10-18
WO2007117331A3 true WO2007117331A3 (en) 2008-05-22

Family

ID=38581529

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2006/062527 WO2007117331A2 (en) 2005-12-22 2006-12-22 Novel dna polymerase from thermoanaerobacter tengcongenesis

Country Status (1)

Country Link
WO (1) WO2007117331A2 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2697372B1 (en) 2011-04-11 2015-12-16 Roche Diagnostics GmbH Dna polymerases with improved activity
EP2788481B1 (en) 2011-12-08 2018-03-07 Roche Diagnostics GmbH Dna polymerases with improved activity
JP6140182B2 (en) 2011-12-08 2017-05-31 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ DNA polymerase with improved activity
WO2013083262A1 (en) 2011-12-08 2013-06-13 Roche Diagnostics Gmbh Dna polymerases with improved activity

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997021821A1 (en) * 1995-12-15 1997-06-19 Amersham Life Science, Inc. Thermostable dna polymerase from thermoanaerobacter thermohydrosulfuricus and mutant enzymes thereof with exonuclease activity removed

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997021821A1 (en) * 1995-12-15 1997-06-19 Amersham Life Science, Inc. Thermostable dna polymerase from thermoanaerobacter thermohydrosulfuricus and mutant enzymes thereof with exonuclease activity removed

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
ADAMS M W W: "ENZYMES AND PROTEINS FROM ORGANISMS THAT GROW NEAR AND ABOVE 100 DEG C", ANNUAL REVIEW OF MICROBIOLOGY, ANNUAL REVIEWS INC., PALO ALTO, CA, US, vol. 47, 1993, pages 627 - 658, XP000603156, ISSN: 0066-4227 *
BAO QIYU ET AL: "A complete sequence of the T. tengcongensis genome", GENOME RESEARCH, COLD SPRING HARBOR LABORATORY PRESS, WOODBURY, NY, US, vol. 12, no. 5, May 2002 (2002-05-01), pages 689 - 700, XP002355673, ISSN: 1088-9051 *
DATABASE EMBL-SVA 16 December 2005 (2005-12-16), XP002473539, retrieved from EBI Database accession no. AE13054.1 *
DATABASE UniProt 1 June 2002 (2002-06-01), XP002473538, retrieved from EBI Database accession no. Q8RBE6 *
PAVLOV A R ET AL: "Recent developments in the optimization of thermostable DNA polymerases for efficient applications", TRENDS IN BIOTECHNOLOGY, ELSEVIER PUBLICATIONS, CAMBRIDGE, GB, vol. 22, no. 5, 1 May 2004 (2004-05-01), pages 253 - 260, XP004504365, ISSN: 0167-7799 *

Also Published As

Publication number Publication date
WO2007117331A2 (en) 2007-10-18

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