WO2007112377A3 - Three-dimensional structure of hdhd4 complexed with magnesium and a phosphate mimetic - Google Patents

Three-dimensional structure of hdhd4 complexed with magnesium and a phosphate mimetic Download PDF

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Publication number
WO2007112377A3
WO2007112377A3 PCT/US2007/064983 US2007064983W WO2007112377A3 WO 2007112377 A3 WO2007112377 A3 WO 2007112377A3 US 2007064983 W US2007064983 W US 2007064983W WO 2007112377 A3 WO2007112377 A3 WO 2007112377A3
Authority
WO
WIPO (PCT)
Prior art keywords
hdhd4
complexed
magnesium
dimensional structure
present
Prior art date
Application number
PCT/US2007/064983
Other languages
French (fr)
Other versions
WO2007112377A2 (en
WO2007112377A9 (en
Inventor
Patricia A Mcdonnell
Keith L Constantine
Herbert E Klei
Stephen R Johnson
Valentina Goldfarb
Kevin Kish
Soong-Hoon Kim
Original Assignee
Bristol Myers Squibb Co
Patricia A Mcdonnell
Keith L Constantine
Herbert E Klei
Stephen R Johnson
Valentina Goldfarb
Kevin Kish
Soong-Hoon Kim
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bristol Myers Squibb Co, Patricia A Mcdonnell, Keith L Constantine, Herbert E Klei, Stephen R Johnson, Valentina Goldfarb, Kevin Kish, Soong-Hoon Kim filed Critical Bristol Myers Squibb Co
Publication of WO2007112377A2 publication Critical patent/WO2007112377A2/en
Publication of WO2007112377A9 publication Critical patent/WO2007112377A9/en
Publication of WO2007112377A3 publication Critical patent/WO2007112377A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/573—Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2299/00—Coordinates from 3D structures of peptides, e.g. proteins or enzymes

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

The present invention relates to a three-dimensional crystalline form of a HDHD4, which was solved to provide a three-dimensional structure of a HDHD4. In one aspect of the present invention, HDHD4 is complexed with magnesium and a phosphate mimetic, such as vanadate. Methods of using the crystalline form, such as screening methods and rational modulator design methods, are also aspects of the present invention and are described. The structures of the present invention also provide insight into observed properties of HDHD4 and other polypeptides.
PCT/US2007/064983 2006-03-27 2007-03-27 Three-dimensional structure of hdhd4 complexed with magnesium and a phosphate mimetic WO2007112377A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US78632306P 2006-03-27 2006-03-27
US60/786,323 2006-03-27

Publications (3)

Publication Number Publication Date
WO2007112377A2 WO2007112377A2 (en) 2007-10-04
WO2007112377A9 WO2007112377A9 (en) 2007-11-15
WO2007112377A3 true WO2007112377A3 (en) 2008-01-10

Family

ID=38541859

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2007/064983 WO2007112377A2 (en) 2006-03-27 2007-03-27 Three-dimensional structure of hdhd4 complexed with magnesium and a phosphate mimetic

Country Status (1)

Country Link
WO (1) WO2007112377A2 (en)

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003006991A1 (en) * 2001-07-12 2003-01-23 Exelixis, Inc. HADHs AS MODIFIERS OF THE p21 PATHWAY AND METHODS OF USE

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003006991A1 (en) * 2001-07-12 2003-01-23 Exelixis, Inc. HADHs AS MODIFIERS OF THE p21 PATHWAY AND METHODS OF USE

Non-Patent Citations (8)

* Cited by examiner, † Cited by third party
Title
ALLEN K N ET AL: "Phosphoryl group transfer: evolution of a catalytic scaffold", TRENDS IN BIOCHEMICAL SCIENCES, ELSEVIER, HAYWARDS, GB, vol. 29, no. 9, September 2004 (2004-09-01), pages 495 - 503, XP004546959, ISSN: 0968-0004 *
ALLEN KAREN N: "Structural enzymology in the haloalkanoic acid dehalogenase superfamily", FASEB JOURNAL, vol. 21, no. 5, April 2007 (2007-04-01), & EXPERIMENTAL BIOLOGY 2007 ANNUAL MEETING; WASHINGTON, DC, USA; APRIL 28 -MAY 02, 2007, pages A43, XP009091238, ISSN: 0892-6638 *
ARAVIND L ET AL: "The HD domain defines a new superfamily of metal-dependent phosphohydrolases.", TRENDS IN BIOCHEMICAL SCIENCES DEC 1998, vol. 23, no. 12, December 1998 (1998-12-01), pages 469 - 472, XP002455935, ISSN: 0968-0004 *
BLUNDELL T L ET AL: "HIGH-THROUGHPUT CRYSTALLOGRAPHY FOR LEAD DISCOVERY IN DRUG DESIGN", NATURE REVIEWS. DRUG DISCOVERY, NATURE PUBLISHING GROUP, BASINGSTOKE, GB, vol. 1, no. 1, January 2002 (2002-01-01), pages 45 - 54, XP009023187, ISSN: 1474-1784 *
CALDERONE V ET AL: "The First Structure of a Bacterial Class B Acid Phosphatase Reveals Further Structural Heterogeneity Among Phosphatases of the Haloacid Dehalogenase Fold", JOURNAL OF MOLECULAR BIOLOGY, LONDON, GB, vol. 335, no. 3, 16 January 2004 (2004-01-16), pages 761 - 773, XP004480546, ISSN: 0022-2836 *
DATABASE PDB (PROTEIN DATA BANK) [online] 18 April 2006 (2006-04-18), JOINT CENTER FOR STRUCTURAL GENOMICS (JCSG): "CRYSTAL STRUCTURE OF PROTEIN C20ORF147 HOMOLOG (17391249) FROM MUS MUSCULUS AT 1.90 A RESOLUTION", XP002455937, retrieved from EBI Database accession no. 2GFH (PDB ID) *
GOHLA ANTJE ET AL: "Chronophin, a novel HAD-type serine protein phosphatase, regulates cofilin-dependent actin dynamics.", NATURE CELL BIOLOGY JAN 2005, vol. 7, no. 1, January 2005 (2005-01-01), pages 21 - 29, XP002455936, ISSN: 1465-7392 *
PEISACH EZRA ET AL: "X-ray crystal structure of the hypothetical phosphotyrosine phosphatase MDP-1 of the haloacid dehalogenase superfamily.", BIOCHEMISTRY 12 OCT 2004, vol. 43, no. 40, 12 October 2004 (2004-10-12), pages 12770 - 12779, XP002455788, ISSN: 0006-2960 *

Also Published As

Publication number Publication date
WO2007112377A2 (en) 2007-10-04
WO2007112377A9 (en) 2007-11-15

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