WO2007109045A1 - Heterocyclic organic compounds for the treatment of in particular melanoma - Google Patents

Abstract

The present invention relates to the discovery that certain compounds inhibit, regulate and/or modulate tyrosine and serine/threonine kinase and kinase-like proteins, such as RAF kinase, a serine/threonine kinase that functions in the MAP kinase signaling pathway, and is concerned with compositions which contain these compounds, and methods of using them to treat tyrosine and serine/threonine kinase and kinase-like dependent diseases, such as angiogenesis, cancer and cardiac hypertrophy. (Formula I).

Description

HETEROCYCLIC ORGANIC COMPOUNDS FOR THE TREATMENT OF IN PARTICULAR MELANOMA

Summary

[001] The present invention relates to the discovery that certain compounds inhibit, regulate and/or modulate tyrosine and serine/threonine kinase and kinase-like proteins, such as RAF kinase, a serine/threonine kinase that functions in the MAP kinase signaling pathway, and is concerned with compositions which contain these compounds, and methods of using them to treat tyrosine and serine/threonine kinase and kinase-like dependent diseases, such as angiogenesis, cancer and cardiac hypertrophy.

Background

[002] Cells communicate various aspects of their extracellular environment to the nucleus by using various signal transduction pathways. Many of these signals are transmitted by protein kinases which activate various factors through the transfer of phosphate groups Disruption of signal transduction by inhibiting appropriate kinase activity can have a clinical benefit as has been demonstrated by imatinib, an inhibitor of bcr-abl kinase, which is marketed as its mesylate salt under the brand GLEEVEC (in the United States) or GLIVEC.

[003] - The MAP kinase signaling pathway is known in the art as one of the pathways for growth factors to send their signal to proliferate from the extracellular environment to the cell nucleus. The growth factors activate transmembrane receptors located on the cell surface which in turn start a cascade whereby RAS is activated and recruits RAF kinase to the membrane where it is activated and in turn activates MEK kinase which then activates ERK kinase. Activated ERK kinase can move to the nucleus where it activates various gene transcription factors. Aberrations in this pathway can lead to altered gene transcription, cellular growth and contribute to tumorogenicity by negatively regulating apoptosis and transmitting proliferative and angiogenic signals. Inhibitors of RAF kinase have been shown to block signaling through the MAP kinase signaling pathway.

[004] The RAF kinase family is known to have three members designated C-RAF, also known as RAF- 1, B-RAF and A-RAF. It has been reported that B-RAF kinase is commonly activated by one of several somatic point mutations in human cancer, including 59% of the melanoma cell lines tested. See, Davies, H. et al, Nature 417, 949-954 (2002). This invention relates to the discovery of a class of compounds that efficiently inhibit one or more members of the RAF kinase family. [005] The RAF kinase inhibiting property of the compounds makes them useful as therapeutic agents for the treatment for proliferative diseases characterized by an aberrant MAP kinase signaling pathway, particularly many cancers characterized by overexpression of RAF kinase or an activating mutation of RAF kinase, such as melanoma having mutated B-RAF, especially wherein the mutated B-RAF is the V599E mutant. The present invention also provides a method of treating other conditions characterized by an aberrant MAP kinase signaling pathway, particularly where B-RAF is mutated, for example benign Nevi moles having mutated B-RAF, with the compounds.

Description

[006] A first aspect of the present invention provides a compound of formula (I)

or a pharmaceutically acceptable salt, ester or prodrug thereof for use as a pharmaceutical wherein each of Ai, A₂, A₃, A₄ is independently selected from N or C-R³ where R³ represents H or a substituent moiety of C and where at least one Of A₁, A₂ and A₄ is N;

X is a linking moiety selected from N-H, substituted amino, O or S;

R¹ is a substituent of the aromatic ring and n is an integer from 0 to 4;

Y and D are independently selected from O, S, CH₂, NH, R^e-substituted C, or R⁶- substituted N,

R⁶ is a substituent of the ring which contains Y and D and r is an integer from 0 to the maximum number of available valencies of the ring;

R² is a substituted or unsubstituted moiety selected from hydrocarbyl and heterocyclic;

T is selected from H, halogen, O-R^θ, S-R⁸, SO-R⁸ SO₂-R⁸, SO₂-N(R⁸)₂, SO₂-N³R¹⁰ and SO₂- halogen, where R⁸ is selected from hydrogen, substituted or unsubstituted aliphatic, cycloaliphatic, heterocyclyl or aryl; and R⁹ is substituted or unsubstituted aliphatic, cycloaliphatic, or aryl, and N^a and R¹⁰ together represent a 4, 5, 6 ,7 or 8-membered heterocyclic ring including the nitrogen N^a; and p is an integer from 0 to 5. [007] A second aspect of the invention provides a compound of formula (I)

or a pharmaceutically acceptable salt, ester or prodrug thereof wherein each of A₁, A₂, A₃, A₄ is independently selected from N or C-R³ where R³ represents H or a substituent moiety of C and where at least one of Ai, A₂ and A₄ is N; X is a linking moiety selected from N-H, substituted amino, O or S; R¹ is a substituent of the aromatic ring and π is an integer from 0 to 4; Y and D are independently selected from O, S, CH₂, NH, R⁶-substituted C, or R⁶- substituted N, R⁶ is a substituent of the ring which contains Y and D and r is an integer from 0 to the maximum number of available valencies of the ring;

R² is a substituted or unsubstituted moiety selected from hydrocarbyl and heterocyclic; T is selected from H, halogen, O-R^β, S-R^B, SO-R⁸ SO₂-R⁸, SO₂-N(R⁸)₂, SO₂-NR¹⁰ and SO₂- halogen, where R⁸ is selected from hydrogen, substituted or unsubstituted aliphatic, cycloaliphatic, heterocyclyl or aryl; and R⁹ is substituted or unsubstituted aliphatic, cycloaliphatic, or aryl, and NR¹⁰ represents a heterocyclic ring including the nitrogen; and p is an integer from O to 5 and wherein the compound is not:

[008] Preferably R¹ is present (n is not 0) and is independently selected from halogen, lower alkyl, halo-lower alkyl, carboxy, esterified carboxy, hydroxy, etherified or esterrfied hydroxy, lower alkoxy, phenyl, substituted phenyl, lower alkanoyl, substituted or unsubstituted amine, amino, mono- or di- substituted amino, amidino, ureido, mercapto, N- hydroxy-amidino, guanidino, amidino-lower alkyl, sulfo, sulfamoyl, carbamoyl, cyano, cyano-lower alkyl, azo (N=N=N) and nitro.

[009] R¹, or each R¹ independently, is preferably selected from OH, O-alkyl, SH, S- alkyl, halogen, substituted or unsubstituted amine, CF₃ and C₁-C₄ alkyl. Most preferably n is 1.^'

[0010] R² is preferably selected from substituted or unsubstituted aliphatic, alicyclic, or aromatic moieties such as cycloalkyl, heterocyclylalkyl, phenyl, pyrrole, imidazole, pyrazole, isoxazole, oxazole, thiazole, pyridazine, pyrimidine, pyrazine, pyridyl, indole, isoindole, indazole, purine, indolizidine, quinoline, isoquinoline, quinazoline, pteridine, quiπolizidiπe. Preferably R² is aromatic. In particular R² is selected from substituted or unsubstituted phenyl, imidazolyl, pyrrolyl, oxazolyl and isoxazolyl, and especially R² is phenyl or substituted phenyl, wherein the substituent include lower alkyl(Ci-C₆), halogen, OH, lower alkoxy, NH₂, SH, S-alkyl, SO-alkyl, SO₂-alkyl, NH-alkyl, N-dialkyl, carboxyl or CF₃

[0011] Thus preferably X-R2-(T)_P represents

T may preferably be selected from halogen, O-alkyl, O-alkyl-halogen, SO₂-R⁸, SO₂-NHR⁸, SO₂-NR¹⁰ and SO₂-halogen where halogen is preferably chlorine.

[0012] R⁸ and R⁹ may preferably be independently selected from lower alkyl, especially C_L C₂, C₃ or C₄ alkyl, cycloalkyl, heterocyclolalkyl, lower alkenyl, lower alkynyl, lower alkoxy, especially methoxy or ethoxy, lower-alkanoyl, carboxy, amino, mono- or di- substituted amino, a cyclic group, for example phenyl, pyrrole, imidazole, pyrazole, isoxazole, oxazole, thiazole, pyridazine, pyrimidine, pyrazine, pyridyl, indole, isoindole, indazole, purine, indolizidine, quinoline, isoquinoline, quinazoline, piperidyl, pteridine, quinolizidine piperazinyl, pyrollidine, morpholinyl and thiomorpholinyl. [0013] Preferably R8 and R9 are substituted or unsubstituted alkyl or substituted or unsubstituted aryl. In particular R8 may represent linear or branched alkyl, cycloalkyl, linear or branched halo-alkyl, alkoxy, carboxyalkyl, or alkylamino.

[0014] Most preferably R2 is phenyl and T is located para to the linking group X.

[0015] Where T is O-R9 and R2 is phenyl, preferably T is located meta to the linking group X.

[0016] Preferably p is 1. [0017] Particularly preferably T is a moiety selected from the formulae (ι) to (x):

(where q is an integer from 1 to 4 and s is an integer from 0 to 4):

[0018] Preferably Ai and A₂ are N, and A₃ and A₄ are C-R³. It is especially preferred that A₃ and A₄ are C-H.

[0019] Preferably X is N-H. [0020] Preferably R¹ is OH. [0021] Preferably Y is CH₂. Preferably D is CH₂. [0022] Preferably each R³ and R⁶ (where present) respectively are independently selected from hydrogen, halogen, lower aliphatic (especially lower alkyl), halo-lower alkyl, carboxy, lower alkoxycarbonyl, hydroxy, etherified or esterified hydroxy, lower alkoxy, optionally substituted alicyclic group or an optionally substituted aromatic group, lower alkanoyloxy, lower alkanoyl, amino, mono- or di- substituted amino, amidino, ureido, mercapto, N-hydroxy-amidino, guanidino, amidino-lower alkyl, sulfo, sulfamoyl, carbamoyl, cyano, cyano-lower alkyl, azo (N=N=N) and nitro. Most preferably r is 0. However, where r is not 0, preferably R⁶, or each R⁶ independently, is preferably selected from OH, O-alkyl, SH, S-alkyl, halogen, CF₃ and C₁-C₄ alkyl.

[0023] In preferred embodiments of the invention the moiety of formula (I). Il

is a tetrahydroquinoline moiety wherein preferably r = 0. Preferably n = 1. Preferably R¹ is OH so that the moiety is a hydroxy tetrahydroquinoline, most especially 1 ,2,3,4- tetrahydroquinolin-5-ol (formula (I. Ill):

[0024] Preferred compounds include compounds of the formulae (II), (III) and (IV):

preferably wherein X is NH, R² is phenyl n is 1 and/or p is 1. [0025] Other preferred compounds have the formula (V):

preferably wherein A₁ and A₂ are N, and A₃ and A₄ are C-R³.

[0026] Further preferred compounds have the formulae (Vl), (VII) and (VIM):

preferably wherein n is 1.

[0027] Still further preferred compounds have the formula (IX)

[0028] For compounds of formulae (Vl) to (IX) preferably p is 1 and X is NH. [0029] Other preferred compounds include: formula (X)

wherein G represents R^B, NHR⁶ or NR¹⁰ and preferably wherein X is NH; formula (Xl);

and formula (XII)

[0030] Preferably in the compound of formula (XlI) T is O-R⁹. [0031] Preferably in compounds (Xl) and (XII) X is NH.

[0032] A third aspect of the invention provides a process for the preparation of a compound of the formula:

which process comprises the following reaction scheme:

where step 2 is optional and where carried out T' is a precursor of T, and R^1' is a precursor of R¹ or is R¹ and X, R¹, R², T and P are as defined in claim 1. [0033] Preferably X is NH.

[0034] Preferably R² is phenyl. [0035] Preferably p is 1. [0036] Preferably R^{1 '} is OH.

[0037] In one preferred embodiment T represents SO₂-G where G represents R⁸, NHR⁸ or NR¹⁰ and R⁸ and R¹⁰ are as defined in the first aspect of the invention.

[0038] In another preferred embodiment T represents O-R⁹ where R⁹ is as defined in the first aspect of the invention.

[0039] Preferably in the latter embodiment X-R²-(T)_P represents

[0040] Another aspect of the present invention is a compound of formula (I)₁ wherein

A₁ , A₂, A₃ and A₄ are N or CR₃, and where at least one of Ai , A₂ and A₄ is N; X is N-R₅, O or S;

R¹ is OH, -O-alkyl, -SH, -S-alkyl, halogen, substituted or unsubstituted amines, -CF₃ or -C-ι-C₄-alkyl;

Y is O, S, CR₅ or NR⁵; D is O, S, CR₅ or NR⁵;

R² is an alkyl, alicycle, heterocycle, aliaromatic, hetereoaromatic all of which may be substituted or unsubstituted; T is H, -SO₂-NH-R⁴ Or -SO₂-R⁴;

R⁴ is H, alkyl or aryl, which may be substituted or unsubstituted; R⁵ is H or alky or C(O)-O-C-Ph; n is 0-4; or a tautomer thereof, or a salt thereof. [0041] Special preference is given to a compound of formula (I), wherein

A₃ and A₄ are CH;

Y is CH₂ or NR⁵ such as -NH-C(O)-O-C-Ph; X is NH;

D is CH₂;

R¹ is OH, Cl, Me or F;

R² is phenyl, imidazolyl, pyrrolyl, oxazolyl or isoxzole, where phenyl may be unsubstituted or substituted with 1 , 2 or 3 -OMe groups, Cl, CF₃, -SMe, OH, -O-[CH₂]₂-pyπ^'dine,

-O-[CH₂]₃-CI or -O-[CH₂]₃-morphoiino; and

R⁴ is H₁ C₂NMe, C₂OH, -Npipeidinyl, Me, Me(t-butyl), C₂COOH or ethyl(isopropyl); R⁵ is H; n is O or 1 ; or a tautomer thereof, or a salt thereof.

K) [0042] More generally, within the context of the present disclosure, the general terms used herein to describe compounds of formulae (I to XII) have the following meanings, unless indicated otherwise.

[0043] Hydrocarbyl may. be defined as having preferably up to 20 carbon atoms, especially up to 12 carbon atoms. Hydrocarbyl groups may be linear or branched aliphatic, e.g. alkyl, alkenyl or alkynyl; they may be alicyclic (i.e. aliphatic-cyclic), e.g. cycloalkyl; they may be aromatic, e.g. phenyl. Hydrocarbyl groups may contain a combination of two or more moieties selected from aliphatic, alicyclic and aromatic moieties, e.g. a combination of at least one alkyl group and an aromatic group. In some instances, hydrocarbyl groups may be optionally interrupted by one or more in-chain heteroatoms, for example -O-, thus forming, for example, an ether linkage.

[0044] A mono- or di- substituted amino moiety may be defined where the amino is optionally substituted by a hydrocarbyl moiety, the hydrocarbyl moiety being, for example, selected from lower alkyl, especially C₁ , C₂, C₃ or C₄ alkyl, cycloalkyl, especially cyclohexyl, alkyl-carboxy, carboxy, lower alkanoyl, especially acetyl, a carbocyclic group, for example cyclohexyl or phenyl, a heterocyclic group; where the hydrocarbyl moiety is unsubstituted or substituted by, for example lower alkyl (Ci, C₂, C₃, C₄, C₅ , C₅ or C₇), halogen, OH, lower alkoxy, NH₂, SH, S-alkyl, SO-alkyl, SO₂-alkyl, NH-alkyl, N-dialkyl, carboxyl, CF₃, wherein alkyl may be optionally substituted branched, unbranched or cyclic C_1-6, interrupted 0-3 times by O, S, N.

[0045] As used herein, the term mercapto defines moieties of the general structure -S- R_θ wherein R_e is selected from H, alkyl, a carbocylic group and a heterocyclic group as described herein.

[0046] As used herein, the term guanidino defines moieties of the general structure - NHR-C(NH)NH₂ and derivatives thereof, in particular, where hydrogen is replaced by alkyl, e.g. methyl or ethyl.

[0047] As used herein, the term amidino defines moieties of the general structure - C(NH)NH₂ and derivatives thereof, in particular, where hydrogen is replaced by alkyl, e.g. methyl or ethyl.

[0048] Alkyl preferably has up to 20, more preferably up to 12 carbon atoms and is linear or branched one or more times; preferred is lower alkyl, especially preferred is C₁-C₄- alkyl, in particular methyl, ethyl or i-propyl or t-butyl. Where alkyl may be substituted by one or more substituents. Unsubstituted alkyl, preferably lower alkyl, is especially preferred.

[0049] Aikyl may be optionally interrupted by one or more in-chain heteroatoms, for example -O-, thus forming, for example, an ether linkage. [0050] Substituted alkyl is alkyl as last defined, especially lower alkyl, preferably methyl; where one or more, especially up to three, substitueπts may be present, primarily from the group selected from halogen, especially fluorine, amino, N-lower alkylamino, N₁N- di-lower alkylamino, N-lower alkanoylamino, hydroxy, cyaπo, carboxy, lower alkoxycarbonyl, and phenyl-lower alkoxycarbonyl. Trifluoromethyl is especially preferred. One class of compounds includes a substituted alkyl where the alkyl is substituted with a heterocyclic ring, for example a pyrazine ring, thus forming an alkylene-het group, i.e. - CH₂-Het, the alkyl group effectively acting as a linker between the heterocycle and a second moiety.

[0051] The term "lower" when referring to substituents such as alkyl, alkoxy, alkyl amine, alkylthio and the like denotes a radical having up to and including a maximum of 7, especially from 1 up to and including a maximum of 4, carbon atoms, the radicals in question being unbranched or branched one or more times.

[0052] The alkyl portion of lower alkyl, lower alkoxy, mono- or di-lower alkyl amino, lower alkyl thio and other substituents with an alkyl portion is especially C₁-C₄alkyl, for example n-butyl, sec-butyl, tert-butyl, n-propyl, isopropyl, methyl or ethyl. Such alkyl substituents are unsubstituted or substituted by halogen, hydroxy, nitro, cyano, lower alkoxy, C₃, C₄, C₅, C₆ or C₇ cycloalkyl, amino, or mono- or di-lower alkyl amino, unless otherwise indicated.

[0053] Halo-lower alkyl, halo-lower alkyloxy, halo-lower alkylthio and the like refer to substituents having an alkyl portion wherein the alkyl portion is mono- to completely substituted by halogen. Halo-lower alkyl, halo-lower alkyloxy, halo-lower alkylthio and the like are included within substituted lower alkyl, substituted lower alkoxy, substituted lower alkylthio and the like.

[0054] Among the moieties corresponding to substituted alkyl, hydroxy-lower alkyl, especially 2-hydroxyethyl, and/or halo-lower alkyl, especially trifluoromethyl or 2,2,2- trifluoroethyl, are especially preferred.

[0055] An alicyclic group is a carbocyclic group especially which comprises 3, 4, 5, 6 or 7 in ring carbon atoms and is non aromatic, but may be saturated or unsaturated. Preferred alicyclic groups comprise cycloalkyl groups, which are preferably C3-C10- cycloalkyl, especially cyclopropyl, dimethylcyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl or cycloheptyl, cycloalkyl being unsubstituted or substituted by one or more, especially 1 , 2 or 3, substituents.

[0056] An aromatic group is heterocyclic or carbocyclic and is bound via a bond located at an aromatic ring carbon atom of the radical (or optionally bound via a linking group, such as -O- or -CH₂-). Preferably the aromatic group is carbocyclic and has a ring system of not more than 16 carbon atoms and is preferably mono- bi- or tri- cyclic and may be fully or partially substituted, for example substituted by at least two substituents. Preferably, the aromatic group is selected from phenyl, naphthyl, iπdenyl, azulenyl and anthryl, and is preferably in each case unsubstituted or substituted by lower alkyl, especially methyl, ethyl or n-propyl, halo (especially fluoro, chloro, bromo or iodo), halo- lower alkyl (especially trifluoromethyl), hydroxy, tower alkoxy (especially methoxy), halo- lower alkoxy (especially 2,2,2-trifluoroethoxy), amino-lower alkoxy (especially 2-amino- ethoxy), lower alkyl (especially methyl or ethyl) carbamoyl, N-(hydroxy-lower alkyl)- carbamoyl (especially N-(2-hydroxyethyl)-carbamoyl) and/or sulfamoyl-substituted aryl, especially a corresponding substituted or unsubstituted phenyl.

[0057] A substituted aromatic group is generally an aromatic group that is substituted with from 1-5, preferably 1 or 2, substituents. Appropriate substituents include, but are not limited to, amino, mono- or di-lower alkyl substituted amino, wherein the lower alkyl substituents may be unsubstituted or further substituted by those substitutents listed above for alkyl groups, halogen, lower alkyl, substituted lower alkyl, hydroxy, lower alkoxy, substituted lower alkoxy, nitro, cyano, mercapto, lower alkylthio, halo-lower alkylthio, heterocyclyl, heteroaryl, heterocyclylalkyl, heteroarylalkyl, lower alkanoyl, carbamoyl, and N-mono- or N,N-di-lower alkyl substituted carbamoyl, wherein the lower alkyl substitents may be unsubstituted or further substituted.

[0058] A heterocycle is an aromatic ring or ring system having 16 or fewer members, preferably a ring of 5 to 7 members. Heterocycle also includes a three to ten membered non-aromatic ring or ring system and preferably a five- or six-membered non-aromatic ring, which may be fully or partially saturated. In each case the rings may have 1 , 2 or 3 hetero atoms selected from the group consisting of nitrogen, oxygen and sulfur. The heterocycle is unsubstituted or substituted by one or more, especially from one to three, for example one, identical or different substituents. Important substituents on heterocycle are those selected from the group consisting of halogen, for example, fluorine or chlorine; mono- or di-lower alkyl-substituted amino wherein the alkyl groups are unsubstituted or substituted by halogen, hydroxy, nitro, cyano, lower alkoxy, C₃-C₇ cycloalkyl, a heterocyclic radical or a heteroaryl radical; lower alkyl, such as methyl or ethyl; halo-lower alkyl, such as trifluoromethyl; lower alkoxy, such as methoxy or ethoxy; halo-lower alkoxy, for example, trifluoromethoxy; lower alkylthio, such as methylmercapto, halo-lower alkylthio, such as trifluoromethylthio, a heteroaryl radical, heteroaryl-lower-alkylene, a heterocyclic radical or heterocyclic-lower-alkylene. [0059] Heterocycle especially is a radical selected from the group consisting of oxiranyl, azirinyl, 1,2-oxathiolanyl, imidazolyl, thienyl, furyl, tetrahydrofuryl, pyranyl, thiopyranyl, thianthrenyl, isobenzofuranyl, benzofuranyl, chromenyl, 2/-/-pyrrolyl, pyrrolyl, pyrrolinyl, pyrrolidinyl, imidazolyl, imidazolidinyl, benzimidazolyl, pyrazolyl, pyrazinyl, pyrazolidinyl, pyranyol, thiazolyl, isothiazolyl, dithiazolyl, oxazolyl, isoxazolyl, pyridyl, pyrazinyl, pyrimidinyl, piperidyl, especially piperidiπ-1-yl, piperazinyl, especially piperazin-1-yl, pyridazinyl, morpholinyl, especially morpholino, thiomorpholiπyl, especially thiomorpholino, indoliziπyl, isoindolyl, 3H-indolyl, indolyl, benzimidazolyl, cumaryl, indazolyl, triazolyl, tetrazolyl, purinyl, 4H-quinolizinyl, isoquinolyl, quinolyl, tetrahydroquinolyl, tetrahydroisoquinolyl, decahydroquinolyl, octahydroisoquinolyl, benzofuranyl, dibenzofuranyl, benzothiophenyl, dibenzothiophenyl, phthalazinyl, naphthyridinyl, quinoxalyt, quinazolinyl, quinazolinyl, cinnoliny!, pteridinyl, carbazolyl, β-carbolinyl, phenanthridinyl, acridinyl, perimidinyl, phenanthrolinyl, furazanyl, phenazinyl, phenothiazinyl, phenoxazinyl, chromenyl, isochromanyl and chromanyl, each of these radicals being unsubstituted or substituted by one to two radicals selected from the group consisting of lower alkyl, especially methyl or tert-butyl, lower alkoxy, especially methoxy, and halo, especially bromo or chloro. Unsubstituted heterocyclyl, especially piperidyl, piperazinyl, thiomorpholino or morpholino, is preferred.

[0060] Halogen is especially fluorine, chlorine, bromine or iodine, more especially fluorine, chlorine or bromine, in particular fluorine.

[0061] Cycloalkyl is preferably C₃-C₁₀-cycloalkyl, especially cyclopropyl, dimethylcyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl or cycloheptyl, cycloalkyl being unsubstituted or substituted by one or more, especially 1 to 3, substituents.

[0062] Heterocyclylalkyl is as cycloalkyl but containing one or more in-ring heteroatoms and may be exemplified by piperidyl, piperazinyl, pyrollidine, morpholinyl.

[0063] Esterified carboxy is especially lower alkoxycarbonyl, such as tert- butoxycarbonyl, iso-propoxycarbonyl, methoxycarbonyl or ethoxycarbonyl, phenyl-lower alkoxycarbonyl, or phenyloxycarbonyl.

[0064] Alkanoyl is primarily alkylcarbonyl, especially lower alkanoyl, e.g. acetyl. In particular, the alkanoyl group may be substituted by substituents, e.g. CO-R

[0065] Any reference to compounds, salts and the like in the plural is always to be understood as including one compound, one salt or the like.

[0066] Throughout the description and claims of this specification, the words "comprise" and "contain" and variations of the words, for example "comprising" and "comprises", means "including but not limited to", and is not intended to (and does not) exclude other moieties, additives, components, integers or steps.

[0067] Any asymmetric carbon atoms may be present in the (R)-, (S)- or (Reconfiguration, preferably in the (R)- or (S)-configuration. Radicals having any unsaturation are present in cis-, trans- or (cis, trans) form. The compounds may thus be present as mixtures of isomers or as pure isomers, preferably as enantiomer-pure diastereomers.

[0068] The invention relates also to possible tautomers of the disclosed compounds.

[0069] Stereoisomeric mixtures, e.g. mixtures of diastereomers, can be separated into their corresponding isomers in a manner known per se by means of suitable separation methods. Diastereomeric mixtures for example may be separated into their individual diastereomers by means of fractionated crystallization, chromatography, solvent distribution, and similar procedures. This separation may take place either at the level of a starting compound or in a compound of Formula I or formulae Il to XII respectively. Enantiomers may be separated through the formation of diastereomeric salts, for example by salt formation with an enantiomer-pure chiral acid, or by means of chromatography, for example by HPLC, using chromatographic substrates with chiral ligands.

[0070] Salts are especially the pharmaceutically acceptable acid addition salts of compounds of formula (I). Such salts are formed, for example, by compounds of formula (I) having a basic nitrogen atom as acid addition salts, preferably with organic or inorganic acids, especially the pharmaceutically acceptable salts. Suitable inorganic acids are, for example, hydrohalic acids, such as hydrochloric acid; sulfuric acid; or phosphoric acid. Suitable organic acids are, for example, carboxylic, phosphonic, sulfonic or sulfamic acids, for example acetic acid, propionic acid, octanoic acid, decanoic acid, dodecanoic acid, glycoiic acid, lactic acid, 2-hydroxybutyric acid, gluconic acid, glucosemonocarboxylic acid, fumaric acid, succinic acid, adipic acid, pimelic acid, suberic acid, azelaic acid, malic acid, tartaric acid, citric acid, glucaric acid, galactaric acid, amino acids, such as glutamic acid, aspartic acid, N-methylglycine, acetylaminoacetic acid, N-acetylasparagine, N- acetylcysteine, pyruvic acid, acetoacetic acid, phosphoserine, 2- or 3-glycerophosphoric acid, maleic acid, hydroxymaleic acid, methylmaleic acid, cyclohexanecarboxylic acid, benzoic acid, salicylic acid, 1- or 3-hydroxynaphthyl-2-carboxylic acid, 3,4,5- trimethoxybenzoic acid, 2-phenoxybeπzoic acid, 2-acetoxybenzoic acid, 4-aminosalicylic acid, phthalic acid, phenylacetic acid, glucuronic acid, galacturonic acid, methane- or ethane-sulfonic acid, 2-hydroxyethanesulfonic acid, ethane-1 ,2-disulfonic acid, benzenesulfonic acid, 2-πaphthaleπesulfoπic acid, 1 ,5-naphthalenedisulfoπic acid, N- cyclohexylsulfamic acid, N-methyl-, N-ethyl- or N-propyl-sulfamic acid, or other organic protonic acids, such as ascorbic acid. [0071] For isolation or purification it is also possible to use pharmaceutically unacceptable salts, for example picrates or perchlorates. Only the pharmaceutically acceptable salts or the free compounds (optionally in the form of pharmaceutical compositions) are used therapeutically, and those are therefore preferred.

[0072] In view of the close relationship between the novel compounds in free form and in the form of their salts, including also those salts which can be used as intermediates, for example in the purification of the novel compounds or for their identification, hereinbefore and hereinafter any reference to the free compounds is also to be understood as including the corresponding salts, as appropriate and expedient.

[0073] The compounds of the present invention are found to inhibit, regulate and/or modulate tyrosine and serine/threonine kinase and kinase-like proteins involved in signal transduction, and compositions containing the compounds are used in the treatment of tyrosine and serine/threonine kinase and kinase-like-dependent diseases, such as angiogenesis, cancer, tumour growth, atherosclerosis, age related macular degeneration, diabetic retinopathy, inflammatory diseases, πeurotraumatic diseases, chronic neurodegeneration, pain, migraine or cardiac hypertrophy, and the like in mammals.

[0074] Specifically, the compounds of the present invention inhibit IKK, PDGF-R, Kdr, c-Src, HeM ₁ Her-2, c-Klt, C-AbI, Ins-r, Tek, FIt-I , Flt-3, Flt-4, c-Abi and FGFR-1 , Eph receptors (e.g. EphB4), CDK1 , CDK2 and RET at >70% inhibition at 10 micromole. More specifically, the compounds inhibit the RAF family of kinases including mutations with IC50 values in the range of 1 -1000 nM.

[0075] Typically, the patient is a mammal, generally a human, suffering from a disease that is characterized by excessive signaling through the MAP kinase pathway. This can be measured by activation state specific antibodies to pathway members by methods such as Western blot analysis or immunohistochemistry. Such methods are known to those of skill in the art.

[0076] In general, the disease characterized by excessive signaling through the MAP kinase signaling pathway is a proliferative disease, particularly a cancer characterized by increased RAF kinase activity, for example one which overexpresses wild-type B- or C- RAF kinase, or that expresses an activating mutant RAF kinase, for example a mutant B- RAF kinase. Cancers wherein a mutated RAF kinase has been detected include melanoma, colorectal cancer, ovarian cancer, gliomas, adenocarcinomas, sarcomas, breast cancer and liver cancer. Mutated B-RAF kinase is especially prevalent in many melanomas. [0077] In accordance with the present invention, a sample of diseased tissue may taken from the patient, for example, as a result of a biopsy or resection, and tested to determine whether the tissue produces a mutant RAF kinase, such as a mutant B-RAF kinase or overexpresses a wild-type RAF kinase, such as wild-type B- or C-RAF kinase. If the test indicates that mutant RAF kinase is produced or that a RAF kinase is overproduced in the diseased tissue, the patient is treated by administration of an effective RAF-inhibiting amount of a RAF inhibitor compound described herein.

[0078] However, it is also possible to downregulate the MAP kinase signaling pathway with a RAF kinase inhibiting compound if another kinase in the cascade is the cause of excessive signaling in the pathway. Thus, the present invention further relates to the treatment of a disease characterized by excessive signaling in the MAP kinase signaling pathway attributed to a cause other than an activating mutation in or overexpression of a RAF kinase.

[0079] Tissue samples are tested by methods generally known in the art. For example, B-RAF mutations are detected by allele specific PCR, DHPLC, mass spectropscopy and overexpression of wild-type B- or C-RAF detected by immunohistochemistry, immunofluorescense, or Western blot analysis. A particularly useful method of detecting B- RAF mutations is a polymerase chain reaction based method. Similar methods are used to determine whether other kinases in the cascade are mutant or overexpressed.

[0080] A particularly important aspect of this invention relates to a method of treating melanoma, which comprises (a) testing melanoma tissue from a patient to determine whether the melanoma tissue expresses mutant RAF kinase or overexpresses a wild-type RAF kinase and (b) treating the patient with an effective RAF kinase inhibiting amount of a RAF-iπhibitiπg compound described herein if the melanoma tissue is found to overexpress a wild type RAF kinase or express an activating mutant B-RAF kinase.

[0081] An important aspect of this embodiment relates to a method of treating melanoma, which comprises (a) testing melanoma tissue from a patient to determine whether the melanoma tissue overexpresses B-RAF kinase or C-RAF kinase activity and (b) treating the patient with an effective RAF kinase inhibiting amount of a RAF inhibiting compound described herein if the melanoma tissue is found to overexpress the B-RAF kinase or C-RAF kinase activity.

[0082] Another important aspect of this embodiment relates to a method of treating melanoma, which comprises (a) testing melanoma tissue from a patient to determine whether the melanoma tissue expresses mutant B-RAF kinase and (b) treating the patient with an effective RAF kinase inhibiting amount of a RAF inhibiting compound described herein if the melanoma tissue is found to express mutant B-RAF kinase. [0083] Generally, the B-RAF kinase mutation is one of those described in the Davies et al article cited. These mutations are summarized in Table 1.

[0084] Thus, the present invention particularly relates to a method of treating a disease characterized by an activated mutant B-RAF kinase, which comprises detecting a mutation in the B-RAF kinase gene or protein in a tissue sample from a patient and treating the patient with an effective B-RAF kinase inhibiting compound, especially a compound described herein.

[0085] Hence, the present invention additionally relates to a compound (for example of formulae I to XII) for use in the treatment of melanoma. More particularly, the invention relates to a compound for use in the treatment of a disease characterized by an activated mutant B-RAF kinase.

Table 1

[0086] Further, the invention provides for the use of a compound (for example of formulae I to XII) in the manufacture of a medicament for use in the treatment of melanoma. More specifically, the invention provides for the use of a compound in the manufacture of a medicament for use in the treatment of a disease characterized by an activated mutant B-RAF kinase.

[0087] An important aspect of this invention includes those instances wherein the mutant B-RAF kinase exhibits a mutation described in Table 1 , especially the V599E mutation.

[0088] A particularly important aspect of this invention includes those instances wherein disease is melanoma and the mutant B-RAF kinase exhibits a mutation described in Table 1 , especially the V599E mutation. [0089] Accordingly, this invention includes a method of treating a disease characterized by mutant B-RAF kinase, which comprises detecting a mutation in the B-RAF kinase gene selected from G1388A, G1388T, G1394C, G1394A, G1394T, G1403C, G1403A, G1753A, T1782G, G1783C, C1786G, T1787G, T1796A and TG1796-97AT, or corresponding mutation in the RAF. kinase protein, in a tissue sample from a patient and treating the patient with an effective B-RAF kinase inhibiting compound described herein.

[0090] The present invention further relates to a method of inhibiting RAF kinase, which comprises contacting the RAF kinase with a compound of formula (I), or more specifically with any one of the compounds of formulae (II) to (XII). Preferably, the RAF kinase is B- or C-RAF kinase, or a mutant RAF kinase, especially a mutant B-RAF kinase, particularly the V599E mutant. The RAF kinase may be isolated or in a cellular environment.

[0091] The compounds of formula (I), and more specifically the compounds of formulae (II) to (XII) have valuable pharmacological properties, as described above.

[0092] The compounds of the present invention may be administered alone or in combination with other anticancer agents, such as compounds that inhibit tumor angiogenesis, for example, the protease inhibitors, epidermal growth factor receptor kinase inhibitors, vascular endothelial growth factor receptor kinase inhibitors and the like; cytotoxic drugs, such as antimetabolites, like purine and pyrimidine analog antimetabolites; antimitotic agents like microtubule stabilizing drugs and antimitotic alkaloids; platinum coordination complexes; anti-tumor antibiotics; alkylating agents, such as nitrogen mustards and nitrosoureas; endocrine agents, such as adrenocorticosteroids, androgens, anti-androgens, estrogens, anti-estrogens, aromatase inhibitors, gonadotropin-releasing hormone agonists and somatostatin analogues and compounds that target an enzyme or receptor that is overexpressed and/or otherwise involved a specific metabolic pathway that is upregulated in the tumor cell, for example ATP and GTP phosphodiesterase inhibitors, protein kinase inhibitors, such as serine, threonine and tyrosine kinase inhibitors, for example, Abelson protein tryosine kinase and the various growth factors, their receptors and kinase inhibitors therefore, such as, epidermal growth factor receptor kinase inhibitors, vascular endothelial growth factor receptor kinase inhibitors, fibroblast growth factor inhibitors, insulin-like growth factor receptor inhibitors and platelet-derived growth factor receptor kinase inhibitors and the like; methionine aminopeptidase inhibitors, proteasome inhibitors, cyclooxygenase inhibitors, for example, cyclooxygenase-1 or -2 inhibitors, and histone deacetylase inhibitors.

[0093] The compound of the present invention may also be administered together with radiotherapy, immunotherapy, surgical treatment or combinations thereof. Treatment to maintain the status of a patient after tumor remission or even chemopreventive treatment, for example in the case of at-risk patients, is also possible.

[0094] Compounds according to the invention are intended not only for the (prophylactic and, preferably, therapeutic) treatment of human beings, but also for the treatment of other warm-blooded animals, for example of commercially useful animals, for example rodents, such as mice, rabbits or rats, or guinea pigs.

[0095] In general, the invention relates also to the use of a compound of formula (I), and more specifically to the use of compounds of formulae (II) to (XII), in inhibiting RAF kinase activity.

[0096] The compounds of the present invention are preferably administered as an active ingredient in a pharmaceutical composition. Preference is given to a pharmaceutical composition which is suitable for administration to a warm-blooded animal, especially a human being or a commercially useful mammal, which is suffering from a disease characterized by an aberrant MAP kinase signaling pathway especially, a tumor disease, most particularly melanoma, comprising a compound of formula (I), or a pharmaceutically acceptable salt thereof where salt-forming groups are present, in an amount that is effective in inhibiting RAF kinase, particularly a mutant RAF kinase, together with at least one pharmaceutically acceptable carrier.

[0097] Preference is given also to a pharmaceutical composition for the prophylactic or, especially, therapeutic treatment of tumor diseases and other proliferative diseases in a warm-blooded animal, especially a human being or a commercially useful mammal, which requires such treatment, especially which is suffering from such a disease, comprising a novel compound of formula (I), or a pharmaceutically acceptable salt thereof, as active ingredient in an amount that is effective prophylactically or, especially, therapeutically against the mentioned diseases.

[0098] Pharmaceutical compositions comprise from approximately 1 % to approximately 95 % active ingredient, dosage forms that are in single dose form preferably comprising from approximately 20 % to approximately 90 % active ingredient, and dosage forms that are not in single dose form preferably comprising from approximately 5 % to approximately 20 % active ingredient. Unit dose forms are, for example, dragees, tablets, ampoules, vials, suppositories or capsules. Other dosage forms are, for example, ointments, creams, pastes, foams, tinctures, lipsticks, drops, sprays, dispersions, etc. Examples are capsules comprising from approximately 0.05 g to approximately 1.0 g of the active ingredient. [0099] The pharmaceutical compositions of the present invention are prepared in a manner employing steps which may individually be known per se, for example by means of conventional mixing, granulating, confectioning, dissolving or lyophilising processes.

[00100] Solutions of the active ingredient are preferably used, in addition also suspensions or dispersions, especially isotonic aqueous solutions, dispersions or suspensions, which, in the case of, for example, lyophilised compositions which contain the active substance alone or together with a carrier, for example mannitol, can be prepared prior to use. The pharmaceutical compositions may be sterilised and/or comprise excipients, for example preservatives, stabilisers, wetting agents and/or emulsifiers, solubilisers, salts for regulating the osmotic pressure and/or buffers, and are prepared in a manner known per se, for example by means of conventional dissolving or lyophilising processes. The mentioned solutions or suspensions may comprise viscosity-increasing substances, such as sodium carboxymethylcellulose, carboxymethylcellulose, dextran, polyvinylpyrrolidone or gelatin, or solubilisers, for example Tween 80 [polyoxyethylene(20)sorbitan monooleate, trade mark of ICI Americas, Inc, USA].

[00101] Suspensions in oil comprise as the oily component the vegetable, synthetic or semi-synthetic oils customary for injection purposes. There may be mentioned as such especially liquid fatty acid esters, which comprise as the acid component a long-chained fatty acid having from 8 to 22, especially from 12 to 22, carbon atoms, for example lauric acid, tridecylic acid, myristic acid, peπtadecylic acid, palmitic acid, margaric acid, stearic acid, arachidic acid, behenic acid or corresponding unsaturated acids, for example oleic acid, elaidic acid, erucic acid, brassidic acid or linoleic acid, optionally with the addition of antioxidants, for example vitamin E, β-carotene or 3,5-di-tert-butyl-4-hydroxytoluene. The alcohol component of those fatty acid esters has a maximum of 6 carbon atoms and is a mono- or poly-hydric, for example mono-, di- or tri-hydric, alcohol, for example methanol, ethanol, propanol, butanol or pentanol or their isomers, but especially glycol and glycerol. Examples of fatty acid esters which may be mentioned are, therefore: ethyl oleate, isopropyl myristate, isopropyl palmitate, "Labrafil M 2375" (polyoxyethyleneglycerol trioleate from Gattefosse, Pans), "Labrafil M 1944 CS" (unsaturated polyglycolised glycerides prepared by alcoholysis of apricot kernel oil and composed of glycerides and polyethylene glycol ester; Gattefosse, France), "Labrasol" (saturated polyglycolised glycerides prepared by alcoholysis of TCM and composed of glycerides and polyethylene glycol ester; Gattefosse, France) and/or "Miglyol 812" (triglyceride of saturated fatty acids having a chain length of from C₈ to C₁₂ from HCiIs AG, Germany), but especially vegetable oils, such as cottonseed oil, almond oil, olive oil, castor oil, sesame oil, soybean oil and, more especially, groundnut oil. [00102] The preparation of the injection compositions is carried out in customary manner under sterile conditions, as are also the introduction thereof, for example, into ampoules or vials and the sealing of the containers.

[00103] Pharmaceutical compositions for oral administration can be obtained, for example, by combining the active ingredient with one or more solid carriers, granulating a resulting mixture, where appropriate, and processing the mixture or granules, if desired, where appropriate by addition of additional excipients, to tablets or dragee cores.

[00104] Suitable carriers are especially fillers, such as sugars, for example lactose, saccharose, mannitol or sorbitol, cellulose preparations and/or calcium phosphates, for example tricalcium phosphate or calcium hydrogen phosphate, also binders, such as starches, for example corn, wheat, rice or potato starch, methylcellulose, hydroxypropylmethylcellulose, sodium carboxymethylcellulose and/or polyvinylpyrrolidone, and/or, if desired, disintegrators, such as the above-mentioned starches, also carboxymethyl starch, crosslinked polyvinylpyrrolidone, alginic acid or a salt thereof, such as sodium alginate. Additional excipients are especially flow conditioners and lubricants, for example silicic acid, talc, stearic acid or salts thereof, such as magnesium or calcium stearate, and/or polyethylene glycol, or derivatives thereof

[00105] Dragee cores can be provided with suitable, optionally enteric, coatings, there being used inter alia concentrated sugar solutions which may contain gum arabic, talc, polyvinylpyrrolidone, polyethylene glycol and/or titanium dioxide, or coating solutions in suitable organic solvents or solvent mixtures or, for the preparation of enteric coatings, solutions of suitable cellulose preparations, such as acetylcellulose phthalate or hydroxypropylmethylcellulose phthalate. Colourings or pigments may be added to the tablets or dragee coatings, for example for identification purposes or to indicate different doses of active ingredient.

[00106] Pharmaceutical compositions for oral administration are also hard gelatin capsules and soft sealed capsules consisting of gelatin and a plasticiser, such as glycerol or sorbitol. The hard gelatin capsules may contain the active ingredient in the form of granules, for example in admixture with fillers, such as corn starch, binders and/or glidants, such as talc or magnesium stearate, and optionally stabilisers. In soft capsules the active ingredient is preferably dissolved or suspended in suitable liquid excipients, such as fatty oils, paraffin oil or liquid polyethylene glycols or fatty acid esters of ethylene glycol or propylene glycol, it likewise being possible to add stabilisers and detergents, for example of the polyoxyethylenesorbitan fatty acid ester type.

[00107] Suitable rectally administrable pharmaceutical compositions are, for example, suppositories that consist of a combinatioⁿ of the active ingredient with a suppository base. Suitable suppository bases are, for example, natural or synthetic triglycerides, paraffin hydrocarbons, polyethylene glycols or higher alkanols.

[00108] For parenteral administration there are suitable, especially, aqueous solutions of an active ingredient in water-soluble form, for example in the form of a water-soluble salt, or aqueous injection suspensions that comprise viscosity-increasing substances, for example sodium carboxymethylcellulose, sorbitol and/or dextran and, if desired, stabilisers. The active ingredient, optionally together with excipients, can also be in the form of a lyophilisate and can be made into a solution prior to parenteral administration by the addition of suitable solvents.

[00109] Solutions used, for example, for parenteral administration can also be used as infusion solutions.

[00110] Preferred preservatives are, for example, antioxidants, such as ascorbic acid, or microbicides, such sorbic acid or benzoic acid.

[00111] The invention relates especially to a process or a method for treating one of the pathological conditions that is characterized by an aberrant MAP kinase signaling pathway, especially a disease responsive to inhibition of RAF kinase, especially a corresponding tumor disease. The compounds of formula (I) can be administered prophylactically or therapeutically as such or in the form of pharmaceutical compositions, preferably in an amount that is effective against the mentioned diseases, to a warm-blooded animal, for example a human being, requiring such treatment, the compounds being used especially in the form of pharmaceutical compositions. In the case of a body weight of approximately 70 kg, a daily dose of from approximately 0.1 g to approximately 5 g, preferably from approximately 0.5 g to approximately 2 g, of a compound of the present invention is administered

[00112] The preferred dosage, composition and preparation of pharmaceutical formulations (medicaments) to be used in each particular case are described above.

[00113] The compounds of the present invention are prepared utilizing methods preferably according to the exemplary reaction schemes described below, individual steps of the said methods being known in general terms to those skilled in the art. [00114] A general scheme showing a process of the present invention is as described above. A more specific variation of the above scheme is given below (Scheme G):

Scheme G

[00115] An example of an R group is one containing sulphur, as illustrated below in Schemes 1 and 2:

2-Phβnylamlno-pyrimldin-4-ot

Synthetic Communications (1997), 27 11 1943-1949

Scheme 1 [00116] A particular example of a reaction of Scheme 1 is shown below:

2-Phenylamlno-pyrlmldlπ-4-ol

Synthetic Communications (1997), 27 11 1943-1949

Scheme 2

[00117] A third reaction scheme according to the present invention is shown below:

Scheme 3 [00118] A particular example of reaction Scheme 3 is shown below in Scheme 4.

Scheme 4

Examples

[00119] The preparative method will now be illustrated by reference to the specific preparations of 1 -{2-[3-(3-Chloro-propoxy)-phenylamino]-pyrimidin-4-yl}-1 ,2,3,4-tetrahydro- quinolιn-5-ol and various 1-{2-[3-(sulphonyi, sulphanyl and sulphonamιno)-phenylamιno]- pyrimidin-4-yl}-1 ,2,3,4-tetrahydro-quinolin-5-ol derivatives.

[00120] Results of melting point tests and mass spectrometry evaluations are also presented.

[00121] 1-{2-[3-(3-Chloro-propoxy)-phenylamino]-pyrimidin-4-yl}-1, 2,3,4- tetrahydro-quinolin-5-ol

[00122] Intermediate synthesis:

[00123] [3-(3-Chloro-propoxy)-phenyl]-(4-chloro-pyrimidin-2-yl)-amine:

2-Mθthylsυlfanyl- 3-{3-Chloro-propoxy)- 2-[3-<3-Chloro-propo)cy) [3-<3-ChlorQ-propo)cy)-phenyIl- ρyrimldin-4-ol phθnylamlπθ -phenylamlno]-pyrlmldln-4-ol (4-chloro-pyr)mldln-2-yl)-amlπe

[00124] Heating 22.98 g (161.64 mmol) 2-methylsulfanyl-pyrimidin-4-ol in 90 mL DMEU to 100° C results in a clear solution. Now, 30 g (161 64 mmol) 3-(3-chloro-propoxy)- phenylamine are added. Heating at 100° C is continued for 15 h. A 10 mL fraction of this reaction mixture is poured onto aqueous sodium bicarbonate and extracted with ethyl acetate. After evaporation of the solvent the brown oil is dissolved in 1 O mL DMEU and 35 mL POCI₃ are added. After heating the reaction mixture at 70° C for 2 h it is carefully poured onto an aqueous bicarbonate solution. Extraction with ethyl acetate followed by flash chromatography on silica (eluent: hexanes ethyl acetate 1 :1) affords 1 .60 g (yield ca. 50%) of the title compound as brown oil.

¹H NMR: (DMSO d₆, 400 MHz): 10.03 (s, 1 H), 8.45 (d, 1 H), 7.44 (t, 1 H), 7.29 (dd, 1 H), 7.21 (t, 1 H), 6.97 (d, 1 H), 6.61 (dd, 1 H), 4.07 (t, 2H), 3 80 (t, 2H), 2.18 (quint, 2H).

[00125] 1-{2-[3-(3-Chloro-propoxy)-phenylamino]-pyrimidin-4-y!}-1 ,2,3,4-tetrahydro- quinolin-5-ol:

3-<3-Chloro-propoxy)- [3^3.chloropropoxy)-phenyf]- 1-{2-[3-{3-Chloro-propoxy)-phβnylamlno] phenylamine (4-chloro-pyrimidin-2-yl)-amine -pyrimidln-4-yl}-1 ,2,3,4-tβtrahydro-quinolιn-5-ol

[00126] A mixture of 200 mg (0.617 mmol) [3-(3-chloro-propoxy)-pheπyl]-(4-chloro- pyrimidin-2-yl)-amine and 100 mg (0.617 mmol) 3-(3-chloro-propoxy)-phenylamiπe is heated neat at 100° C for 20 minutes. With the help of sonication the resulting resin is dissolved in a mixture of ethyl acetate and aqueous sodium bicarbonate. The organic layer is dried over sodium sulfate and evaporated. Chromatography on silica using dichloromethane / ethyl acetate (10:1 ) as eluent afforded 160 mg (yield 58%) of the title compound as yellow foam.

¹H NMR (DMSO d₆, 400 MHz): 9.43 (s, 1 H)₁ 9.13 (s, br, 1 H), 7.91 (d, 1 H), 7.47 (m, 1 H), 7.17 (d, 1 H), 7.04 (t, 1 H), 6.90 (t, 1 H), 6.72 (d, 1 H), 6.51 (d, 1 H), 6.42 - 6.38 (m, 2H), 3.96 (t, 2H), 3.88 (dd, 2H)₁ 3.71 (t, 2H), 2.53 (t, 2H), 2.09 (quint, 2H), 1.78 (m, 2H). MS: ES+ : 41 1 (M+1)⁺ isotop pattern for 1 chlorine atom.

[00127] 1-{2-[3-(sulphonyl, sulphanyl and sulphonamino)-phenylamino]-pyrimidin- 4-yl}-1 ,2,3,4-tetrahydro-quinolin-5-ol derivatives

[00128] Intermediate synthesis:

2-Phenylamino-pyrimidin-4-ol

Synthetic Communications (1997), 27(11), 1943-1949

4-(4-Chloro-pyrimidin-2-ylamino)- benzenesulfonyl chloride

[00129] (4-Chloro-pyrimidιne-2-yl)-phenyl-amine

[00130] 2-Phenylamino-pyrimidine-4-ol (1.309 g, 7 mmol) is suspended in 35 ml. of acetonitrile and treated with 3.5 mL (14 mmol) of a 4 m solution of hydrochloric acid in dioxane (Aldπch) and 1 6 mL (17.5 mmol) phosphorous oxychloride under nitrogen and at room temperature. The mixture is heated under reflux for 3 hours, cooled and diluted with ethyl acetate. The resulting solution is washed with saturated sodium bicarbonate solution and brine, dried over sodium sulfate and evaporated. The residue is purified by flash chromatography on silica gel using ethyl acetate/hexane 2:8 The title compound is obtained in 86% yield (1.5 g). m.p. 134-135 ⁰C; MS (ES+) m/z (M + H)⁺¹ 206.

[00131] 4-(4-Chloro-pyrimidine-2-ylamino)-benzenesulfonyl chloride

[00132] 3.2 mL (48 mmol) Chlorosulfonic acid are cooled to 0 ⁰C under nitrogen. To this is added (4-chloro-pyrimidine-2-yl)-phenyl-amine (1.15 g, 5.6 mmol) in small portions under stirring. After complete addition, the mixture is stirred 15 minutes at 0 ⁰C, 2 hours at room temperature and 15 minutes at 60 ⁰C. The yellow solution is cooled and added slowly onto 100 g of crashed ice. After the ice has completely melted the solid is filtered off, washed with water and dried under vacuum. The title compound is obtained in 74% yield (1.26 g): m.p. 192-195 ⁰C; MS (ES+) m/z (M + H)⁺¹ 300 (mass of the corresponding methyl sulfonate since the MS solution was made up in methanol).

4-{4-Chloro-pyrimidin-2-ylamino)-be nzenesulfonyl chloride

[00133] N-Cyclohexyl-4-[4-(5-hydroxy-3,4-dιhydro-2H-quinolin-1 -yl)-pyrimidιne-2- ylamino]-beπzenesulfonamide

[00134] A mixture of 560 mg (1.5 mmol) 4-(4-chloro-pyrimidine-2-ylamino)-N-cyclohexyl- benzenesulfonamide and 225 mg (1.52 mmol) 1 ,2,3,4-tetrahydro-quinoline-5-ol is heated without solvent in an oil bath for 15 minutes at 200 ⁰C. The brown viscous mixture is cooled first to room temperature, then with dry ice, and the solidified material is pulverized. This solid is stirred with 5% citric acid solution, filtered, re-suspended in saturated sodium bicarbonate solution, filtered again and finally washed with water. This material is subjected to a flash chromatography on silica gel using ethyl acetate/hexane 8:2. Pure fractions are pooled and evaporated, stirred a few minutes in methanol, filtered, re-suspended in a mixture of toluene and diispropyl ether, filtered again and dried under vacuum. The title compound is obtained in 20% yield (150 mg): m.p. 236-238 ⁰C; MS (ES+) m/z (M + H)⁺¹ 480.

[00135] Starting material 4-(4-chloro-pyrimidine-2-ylamino)-N-cyclohexyl- benzenesulfonamide [00136] 600 mg (2 mmol) 4-(4-Chloro-pyrimidine-2-ylamino)-benzeπesulfonyl chloride are suspended in 60 ml_ of dichloromethane and treated at room temperature with 0.57 ml_ (5 mmol) cyclohexylamine. All the material goes slowly into solution and after stirring for about 15 minutes fine needles start to appear. The stirring is continued for a total of 2 hours then the mixture is diluted with dichloromethane and washed with 5% citric acid and brine. The organic phase is dried (Na₂SO₄) and evaporated. The title compound is obtained in 99% yield (706 mg): m.p. 202-204 ⁰C, MS (ES+) m/z (M + H)⁺¹ 367.

[00137] The following examples are synthesized using an analogous sequence as described for N-cyclohexyl-4-[4-(5-hydroxy-3,4-dihydro-2H-quinolin~1 -yl)-pyrimidine-2- ylaminoj-benzeπesulfoπamide:

Ino]-N-

-yl) lfo

In O]-N - ldo

[00138] (3-Chloropheπyl)-[4-(3,4-dihydro-2H-quinolin-1 -yl)-pyrimidin-2-yl]-amine hydrochloride:

2-Methyl

(3-Chloro-phenyl)-[4-(3,4-dihydro-2 H-quinolin-1-yl)-pyrimidin-2-yl]-amine

[00139] 2-(3-Chloro-pheπylamiπo)-pyrimidine-4-ol

[00140] 2-Methylsulfaπyl-pyrimidiπe-4-ol (568 mg, 4 mmol) and 3-chloronaniliπe (0.47 mL, 4 mL) are mixed and heated for 30 minutes to 170 ⁰C. The resulting solution is cooled and triturated with 0.1 m hydrochloric acid, filtered, washed with water and dried under vacuum. The title compound is obtained in 59% yield (520 mg): m.p. 250-252 ⁰C; MS (ES+) m/z (M + H)⁺¹ 222. [00141] (3-Chloro-pheny])-(4-chloro-pyrimidine-2-yl)-amine

[00142] 2-(3-Chloro-phenylamino)-pyrimidine-4-ol (444 mg, 2 mmol) is added in portions to 6 mL phosphorous oxychloride at room temperature. The mixture is heated to 70 ⁰C for 1 hour, cooled and the excess phosphorous oxychloride evaporated under reduced pressure. The residue is dissolved in ethyl acetate washed with saturated sodium carbonate solution and brine, dried over sodium sulfate and evaporated. The title compound is obtained in 91 % yield (440 mg): m.p. 112-1 14 ⁰C; MS (ES+) m/z (M + H)⁺¹ 240,242.

[00143] (3-Chloro-phenyl)-[4-(3,4-dihydro-2H-quinoline-1-yl)-pyrimidin-2-yl]-amine hydrochloride

[00144] (3-Chloro-phenyl)-(4-chloro-pyrimidine-2-yl)-amine (360 mg, 1.5 mmol) in 1 mL of dioxane is treated with 223 mg (1.5 mmol) 1 ,2,3,4-tetrahydro-quinoltne-5-ol. The mixture is heated 2 hours at 80 ⁰C and then 18 hours at 100 ⁰C. The solvent was evaporated and the residue suspended in ethyl acetate/hexane 1 :1 stirred for a few minutes and filtered. The title compound is obtained in 29% yield (150 mg): m.p. 250-252 ⁰C; MS (ES+) m/z (M + H)⁺¹ 353.

[00145] The following examples are synthesized using an analogous sequence as described for (3-Chlorophenyl)-[4-(3,4-dihydro-2H-quinolin-1-yl)-pyrιmιdιn-2-yl]-amine hydrochloride. The compounds in the table are isolated as the free bases.

lam Ino]-

[00146] 1 -[2-(4-Methanesulfoπyl-phenylamiπo)-pyrimidin-4-yl]-1 ,2,3,4-tetrahydro- quinolin-5-ol (oxidation product of 1-[2-(4-methylsulfanyl-phenylamino)-pyrimidin-4-yl]- 1 ,2,3,4-tetrahydro-quinoline-5-ol)

1 -[2-(4-Methanesulfonyl- prϊenylamiπo)-pyrimidiπ-4-yl]-1,2_I3,4- tθtrahydro-quiπol i π-5-ol

[00147] 1-[2-(4-Methy!sulfanyl-phenylamiπo)-pyrimidin-4-yl]-1 ,2,3,4-tetrahydro-quinoline- 5-ol (364 mg, 1 mmol) is suspended in 10 mL of dichloromethane at 0 ⁰C. m- chloroperbenzoic acid (FLUKA 25800, 590 mg, 2.4 mmol) is added and the mixture stirred at 0 ⁰C for 45 minutes. 100 mg of Na₂SO₃ are added and the reaction mixture is then partitioned between dichloromethane and water. The organic layer is separated and washed with saturated sodium bicarbonate, water and brine, dried over sodium sulfate and evaporated. The crude material is purified first by flash chromatography on silica gel using ethyl acetate and then by MPLC on a reverse phase column using an acetonitrile/water gradient containing 0.5% TFA. The title compound is obtained in 6% yield (25 mg): m.p. 242-245 ⁰C; MS (ES+) m/z (M + H)⁺¹ 353.

[00148] Additional compounds within the scope of the present invention include the following:

1-[2-(3,4,5-trimethoxy-phenylamino)-pyrimidin-4-yl]-1 ,2,3,4-tetrahydro-quinolin-5-ol

4-[4-(5-hydroxy-3,4-dihydro-2H-quinolin-1-yl)-pyrimidin~2-ylamino]-benzenesulfonamide

1-[2-(3,4,5-trimethoxy-phenylamino)-pyrir^i;din-4-yl]-1 ,2,3,4-tetrahydrσ-quiπoliπ-6-ol 1-[2-(3,5-dimethoxy-phenylamino)-pyrimidin-4-yl]-1 ,2,3,4-tetrahydro-quinolin-5-ol

1-[2-(3,4,5-trimethoxy-phenylamiπo)-pyrimidin-4-yl]-1 ,2,3,4-tetrahydro-quinolin-7-ol

4-[4-(6-hydroxy-3,4-dihydro-2H-quinolin-1-yl)-pyrimidin-2-ylamino]-Λ/-(2-hydroxy-ethyl)- benzenesulfonamide {4-[4.(5-hydroxy-3,4-dihydro-2/-/-quiπoliπ-1-yl)-pyrimidin-2-ylamino]-beπzenesulfonylamino}- acetic acid

1-[2-(3,5-dimethoxy-phenylamino)-pyrimidin-4-yl]-1 ,2,3,4-tetrahydro-quinolin-6-ol 4-[4-(6-hydroxy-3,4-dihydro-2H-quinolin-1-yl)-pyrimidin-2-ylamino]-benzenesulfonamide 4-[4-(5-hydroxy-3,4-dihydro-2H-quinolin-1-yl)-pyrimidin-2-ylamino]-Λ/-(2-hydroxy-ethyl)-3- methyl-benzenesulfonamide

1-[2-(3-chloro-phenylamino)-pyrimidin-4-yl]-1 ,2,3,4-tetrahydro-quinolin-5-ol [4-(3,4-dihydro-2/-/-quinolin-1-yl)-pyrimidin-2-yl]-(3,4,5-trimethoxy-phenyl)-amine 1-[2-(4-methanesulfoπyl-phenylamino)-pyπmidin-4-yl]-1 ,2,3,4-tetrahydro-quinolin-7-ol 1-[2-(4-methylsulfaπyl-phenylamino)-pyrimidin-4-yl]-1 ,2,3,4-tetrahydro-quinolin-5-ol 1-(2-phenylamino-pyrimidin-4-yl)-1 ,2,3,4-tetrahydro-quinoliπ-5-ol 1-[2-(3-hydroxy-phenylamino)-pyrimidin-4-yl]-1 ,2,3,4-tetrahydro-quinolin-5-ol 4-[4-(5-hydroxy-3,4-dihydro-2H-quinolin-1-yl)-pyrimidin-2-ylamino]-3-methyl-Λ/-(3-nnethyl- butyl)-benzenesulfonamide 4-[4-(5-hydroxy-3,4-dihydro-2H-quinolin-1-yl)-pyrimidin-2-ylamino]-Λ/-pyridiπ-4-ylmethyl- benzenesulfonamide 1-{2-[3-(2-imidazol-1 -yl-ethoxy)-phenylamino]-pyrimidin-4-yl}-1 ,2_I3_l4-tetrahydro-quinolin-5- ol

1-{2-[3-(3-chloro-propoxy)-phenylamino]-pyrimidin-4-yl}-1 ,2,3,4-tetrahydro-quinolin-6-ol 4-[4-(5-chloro-3,4-dihydro-2H-quinolin-1;yt)-pyrimidin-2-ylamino]-benzenesulfonamide 1-{2-[3-(3-morpholin-4-yl-propoxy)-phenylamiπo]-pyrimidιn-4-yl}-1 ,2,3,4-tetrahydro-quinolin-

5-ol

4-[4-(3,4-dihydro-2H-quinolin-1 -yl)-pyrimidin-2-ylamino]-benzenesulfonamide 4-[4-(6-hydroxy-3,4-dihydro-2H-quinolin-1-yl)-pyπmidin-2-ylamino]-Λ/-(3-methyl-butyl)- benzenesulfonamide

[4-(6-methyl-3_l4-dihydro-2/-/-quinolin-1-yl)-pyrimidin-2-yl]-(3,4_l5-trimethoxy-phenyl)-amine 1-{2-[3-(3-chloro-propoxy)-phenylamino]-pyrimidin-4-yl}-1 ,2,3,4-tetrahydro-quinolin-7-ol [4-(7-methyl-3,4-dihydro-2H-quinolin-1-yl)-pyrimidin-2-yl]-(3,4,5-trimethoxy-phenyl)-amine 4-[4-(6-methyl-3,4-dihydro-2H-quinolin-1-yl)-pyrimidin-2-ylamino]-benzenesulfonamide [4-(3,4-dihydro-2/-/-quinolin-1-yl)-pyrimidin-2-yl]-(2,3-dimethoxy-benzyl)-amine 4-[4-(5-hydroxy-3,4-dihydro-2H-quinolin-1-yl)-6-methyl-pyrimidin-2-yIamino]- beπzenesulfonamide S-H-CS^-dihydro^Z-Z-quinolin-i-yO-pyrimidin^-ylaminoJ-phenol 4-[4-(6-fluoro-2-methyl-3,4-dihydro-2/-/-quinolin-1-yl)-pyrimidin-2-ylamino]-Λ/-(3-methyl- butyl)-benzenesulfonamide

1-[2-(3,4,5-trimethoxy-phenylamino)-pyrιmidin-4-yl]-1 ,2,3,4-tetrahydro-quinolin-8-ol [1-(2-phenylamiπo-pyrimidiπ-4-yl)-1 ,2,3,4-tetrahydro-quinolin-3-yl]-carbamic acid benzyl ester 1-[2-(4-trifluoromethyl-phenylamino)-pyrimidin-4-yl]-1 ,2,3,4-tetrahydro-quinolin-5-ol

[00149] Dry-filled capsules

[00150] 5000 capsules, each comprising as active ingredient 0.25 g of one of the compounds of formula I mentioned above, are prepared as follows:

[00151] Composition active ingredient 1250 g talcum 18O g wheat starch 12O g magnesium stearate 80 g lactose 20 g [00152] Preparation process

[00153] The mentioned substances are pulverized and forced through a sieve of 0.6 mm mesh size. 0.33 g portions of the mixture are introduced into gelatin capsules using a capsule-filling machine.

[00154] Soft capsules

[00155] 5000 soft gelatin capsules, each comprising as active ingredient 0.05 g of one of the compounds of formula (I) mentioned above, are prepared as follows:

[00156] Composition active ingredient 25O g PEG 400 1 L Tween 80 1 L

[00157] Preparation process

[00158] The active ingredient is pulverized and suspended in PEG 400 (polyethylene glycol having an Mr of from approximately 380-420, Fluka, Switzerland) and Tween^®80 (polyoxyethylene sorbitan monolaurate, Atlas Chem. Ind. Inc., USA, supplied by Fluka, Switzerland) and ground in a wet pulverizer to a particle size of approximately from 1 -3 μm. 0.43 g portions of the mixture are then introduced into soft gelatin capsules using a capsule-filling machine.

[00159] EQUIVALENTS

[00160] While the invention has been described in connection with what is presently considered to be the most practical and preferred embodiment, it is to be understood that the invention is not to be limited to the disclosed embodiment, but on the contrary, is intended to cover various modifications and equivalent arrangements included within the spirit and scope of the appended claims.

Claims

1. A compound of formula (I)

or a pharmaceutically acceptable salt, ester or prodrug thereof for use as a pharmaceutical wherein each of A₁, A₂, A₃, A₄ is independently selected from N or C-R³ where R³ represents H or a substituent moiety of C and where at least one Of A₁, A₂ and A₄ is N; X is a linking moiety selected from N-H, substituted amino, O or S; R¹ is a substituent of the aromatic ring and n is an integer from 0 to 4; Y and D are independently selected from O, S, CH₂, NH, R⁶-substituted C, or R⁶- substituted N, R⁶ is a substituent of the ring which contains Y and D and r is an integer from 0 to the maximum number of available valencies of the ring;

R² is a substituted or unsubstituted moiety selected from hydrocarbyl and heterocyclic; T is selected from H, halogen, O-R⁹, S-R^a, SO-R⁸ SO₂-R⁸, SO₂-N(R⁸)₂, SO₂-NR¹⁰ and SO₂- halogen, where R^e is selected from hydrogen, substituted or unsubstituted alkyl, cycloalkyl, heterocyclyl or aryl; and R⁹ is substituted or unsubstituted alkyl, cycloalkyl, or aryl, and NR¹⁰ represents a heterocyclic ring including the nitrogen; and p is an integer from O to 5.

2. A compound of formula (I)

or a pharmaceutically acceptable salt, ester or prodrug thereof wherein ' each Of A₁, A₂, A₃, A₄ is independently selected from N or C-R³ where R³ represents H or a substituent moiety of C and where at least one of A-i, A₂ and A₄ is N; X is a linking moiety selected from N-H, substituted amino, O or S; R¹ is a substituent of the aromatic ring and n is an integer from 0 to 4; Y and D are independently selected from O₁ S, CH₂, NH, R⁶-substituted C₁ or R⁸- substituted N, R^θ is a substituent of the ring which contains Y and D and r is an integer from 0 to the maximum number of available valencies of the ring;

R² is a substituted or unsubstituted moiety selected from hydrocarbyl and heterocyclic; T is selected from H, halogen, O-R⁹, S-R⁸, SO-R⁸ SO₂-R⁸, SO₂-N(R⁸J₂, SO₂-NR¹⁰ and SO₂- halogen, where R⁸ is selected from hydrogen, substituted or unsubstituted alkyl, cycloalkyl, heterocyclyl or aryl; and R⁹ is substituted or unsubstituted alkyl, cycloalkyl, or aryl, and NR¹⁰ represents a heterocyclic ring including the nitrogen; and p is an integer from O to 5 and wherein the compound is not:

3. A compound of claim 1 or 2 wherein A₁ and A₂ are N, and A₃ and A₄ are C-R³

4. A compound of claim 1 , 2 or 3 wherein and A₃ and A₄ are C-H.

5. A compound of any of claims 1 to 4 wherein X is N-H.

6. A compound of any one of claims 1 to 5 wherein R¹, or each R¹ independently, is selected from OH, O-alkyl, SH, S-alkyl, halogen, substituted or unsubstituted amine, CF₃ and Ci-C₄ alkyl.

7. A compound as claimed in claim 6 wherein R¹ is OH.

8. A compound of any preceding claim wherein n is 1.

9. A compound of any preceding claim wherein Y is CH₂.

10. A compound of any preceding claim wherein D is CH₂.

1 1. A compound of any preceding claim wherein R² is selected from substituted or unsubstituted aliphatic, alicyclic, or aromatic moieties.

12. A compound of any preceding claim wherein R² is aromatic.

13. A compound as claimed in claim 12 wherein R² is selected from substituted or unsubstituted phenyl, imidazolyl, pyrrolyl, oxazolyl and isoxazolyl.

14. A compound as claimed in claim 12 wherein R² phenyl.

15. A compound as claimed in any preceding claim wherein p is 1.

16. A compound as claimed in claim 14 wherein p is 1 and T is located para- to the linking group X.

17. A compound as claimed in any preceding claim wherein T is selected from halogen, O-alkyl, O-alkyl-halogeπ, SO₂-R⁸, SO₂-NHR⁸, SO₂-NR¹⁰ and SO₂-halogen.

18. A compound as claimed in claim 17 wherein halogen is chlorine.

19. A compound as claimed in claim 17 wherein R⁸ is substituted or unsubstituted alkyl or substituted or unsubstituted aryl.

20. A compound as claimed in claim 17 wherein R⁸ represents linear or branched alkyl, cycloalkyl, linear or branched halo-alkyl, alkoxy, carboxyalkyl, or alkylamino.

21. A compound as claimed in claim 17 wherein T is a moiety selected from the formulae (i) to (x):

where q is an integer from 1 to 4 and s is an integer from 0 to 4

22. A compound as claimed in any preceding claim wherein r is 0.

23. A compound as claimed in claim 1 or 2 selected from compounds of the formulae (II), (III) and (IV):

24. A compound as claimed in claim 23 wherein X is NH.

25. A compound as claimed in claim 23 or 24 wherein R² is phenyl.

26. A compound as claimed in claim 23, 24 or 25 wherein n is 1.

27. A compound as claimed in claim 23, 24, 25 or 26 wherein p is 1.

28. A compound as claimed in claim 1 or 2 of the formula (V):

29. A compound as claimed in claim 28 wherein A₁ and A₂ are N, and A₃ and A₄ are C-R²

30. A compound as claimed in claim 1 or 2 selected from compounds for the formulae (Vl), (VII) and (VIII):

31. A compound as claimed in claim 30 wherein n is 1.

32. A compound as claimed in claim 1 or 2 of the formula (IX)

33. A compound as claimed in claim 30, 31 or 32 wherein p is 1.

34. A compound as claimed in claim 30, 31 , 32 or 33 wherein X is NH.

35. A compound as claimed in claim 1 or 2 of the formula (X)

wherein G represents R 8 , M NLHJDRS _Λ or, N M ERJ 10

36. A compound as claimed in claim 35 wherein X is NH.

37. A compound as claimed in claim 1 or 2 of the formula (Xl)

38. A compound as claimed in claim 1 or 2 of the formula (XII)

39. A compound as claimed in claim 38 wherein T is O-R⁹

40. A compound as claimed in claim 37, 38 or 39 wherein X is NH.

41. A compound as claimed in any preceding claim for use in the treatment of one or more of tyrosine and serine/threonine kinase and kinase-like dependant diseases.

42. The compound of any one of claims 1 to 40 for use in inhibiting IKK, PDGF-R, Kdr, c- Src, HeM , Her-2, c-Kit, c-Abl, Ins-r, Tek, FIt-I , Flt-3, Flt-4, c-Abi and FGFR-1 , Eph

^• receptors (e.g. EphB4), CDK1 , CDK2 and RET activity in a warm-blooded animal.

43. A compound as claimed in claim 42 for use in inhibiting RAF kinase activity in a warm blooded animal.

44. A compound as claimed in claim 41 wherein said diseases are selected from one or more of angiogenesis, cancer, tumour growth, atherosclerosis, age related macular degeneration, diabetic retinopathy, inflammatory diseases, neurotraumatic diseases, chronic neurodegeneration, pain, migraine or cardiac hypertrophy.

45. A compound of any preceding claim for use in the treatment of melanoma.

46. A compound of any preceding claim for use in the treatment of a disease characterized by an activated mutant B-RAF kinase.

47. The use of a compound of any of claims 1 to 40 for the manufacture of a medicament for use in the treatment of tyrosine and serine/threonine kinase and kinase-like- dependent diseases.

48. The use of a compound of any one of claims 1 to 40 for the manufacture of a medicament for use in the treatment of angiogenesis, cancer, tumour growth, atherosclerosis, age related macular degeneration, diabetic retinopathy, inflammatory diseases, neurotraumatic diseases, chronic neurodegeneration, pain, migraine or cardiac hypertrophy.

49. The use of a compound of any one of claims 1 to 40 for the manufacture of a medicament for use in the treatment of melanoma.

50. The use of a compound of any one of claims 1 to 40 for the manufacture of a medicament for use in the treatment of a disease characterized by an activated mutant B-RAF kinase.

51. The use as claimed in any one of claims 47 to 50 wherein said compound is administered alone.

52. The use as claimed in any one of claims 47 to 50 wherein said compound is administered in combination with at least one other anticancer agent.

53. The use as claimed in claim 52 wherein said other at least one other anticancer agent is chosen from protease inhibitors, epidermal growth factor receptor kinase inhibitors, vascular endothelial growth factor receptor kinase inhibitors, cytotoxic drugs, antimitotic agents, platinum coordination complexes, anti-tumor antibiotics, alkylating agents, endocrine agents, androgens, anti-androgens, estrogens, anti-estrogens, aromatase inhibitors, gonadotropin-releasing hormone agonists and somatostatin analogues and compounds that target an enzyme or receptor that is overexpressed and/or otherwise involved a specific metabolic pathway that is upregulated in the tumor cell, protein kinase inhibitors, threonine and tyrosine kinase inhibitors, epidermal growth factor receptor kinase inhibitors, vascular endothelial growth factor receptor kinase inhibitors, fibroblast growth factor inhibitors, insulin-like growth factor receptor inhibitors, platelet-derived growth factor receptor kinase inhibitors, methionine aminopeptidase inhibitors, proteasome inhibitors, cyclooxygenase inhibitors, and histone deacetylase inhibitors.

54. A pharmaceutical composition comprising a compound of any one of claims 1 to 40.

55. A pharmaceutical composition as claimed in claim 54 comprising from approximately 1% to approximately 95% of a compound of any one of claims 1 to 40.

56. A pharmaceutical composition of claim 54 or 55 comprising from approximately 20% to approximately 90% of a compound of any one of claims 1 to 40.

57. A pharmaceutical composition of claim 54, 55 or 56 comprising from approximately 5% to approximately 20% active ingredient.

58. A pharmaceutical composition of any one of claims 54 to 57 for administration by injection.

59. A pharmaceutical composition of claim 58 comprising a solution, suspension or dispersion of a compound of any one of claims 1 to 40.

60. A pharmaceutical composition of claim 58 or 59 additionally comprising a carrier.

61. A pharmaceutical composition of claim 60 wherein said carrier comprises mannitol.

62. A pharmaceutical composition of claim 59, 60 or 61 comprising a suspension in oil.

63. A pharmaceutical composition of any one of claims 54 to 57 for oral administration.

64. A pharmaceutical composition of claim 63 additionally comprising a solid carrier.

65. A pharmaceutical composition of claim 64 additionally comprising gelatin and a plasticiser.

66. A pharmaceutical composition of any one of claims 54 to 57 for rectal administration.

67. A pharmaceutical composition of claim 66 additionally comprising a suppository base.

68. A pharmaceutical composition comprising a compound of any of claims 1 to 40 and at least one anticancer agent.

69. The combination of the compound of any of claims 1 to 40 with at least one anticancer agent.

70. A pharmaceutical composition as claimed in claim 68 or a combination as claimed in claim 69 wherein the anticancer agent is selected from protease inhibitors, epidermal growth factor receptor kinase inhibitors, vascular endothelial growth factor receptor kinase inhibitors, cytotoxic drugs, antimitotic agents, platinum coordination complexes, anti-tumor antibiotics, alkylating agents, endocrine agents, androgens, anti-aπdrogens, estrogens, anti-estrogens, aromatase inhibitors, gonadotropin- releasing hormone agonists and somatostatin analogues and compounds that target an enzyme or receptor that is overexpressed and/or otherwise involved a specific metabolic pathway that is upregulated in the tumor cell, protein kinase inhibitors, threonine and tyrosine kinase inhibitors, epidermal growth factor receptor kinase inhibitors, vascular endothelial growth factor receptor kinase inhibitors, fibroblast growth factor inhibitors, insulin-like growth factor receptor inhibitors, platelet-derived growth factor receptor kinase inhibitors, methionine aminopeptidase inhibitors, proteasome inhibitors, cyclooxygenase inhibitors, and histone deacetylase inhibitors.

71. A method of treating a tyrosine, serine/threonine kinase or kinase-like-dependent . disease comprising administering to a warm-blooded animal, for example a human, a therapeutically effective amount of a compound as claimed in any one of claims 1 to 40.

72. A method of claim 71 which further comprises administering at the same time, or at a separate time, one or more anti-cancer agents.

73. A method of treating melanoma, which method comprises

(a) testing melanoma tissue from a patient to determine whether the melanoma tissue expresses mutant RAF kinase or overexpresses a wild-type RAF kinase, and

(b) treating the patient with an effective RAF kinase inhibiting amount of a RAF inhibiting compound as claimed in any one of claims 1 to 40 if the melanoma is found to overexpresses a wild type RAF kinase or express an activating mutant B- RAF kinase

74. A method of treating melanoma, which method comprises

(a) testing melanoma tissue from a patient and determining whether the melanoma tissue overexpresses B-RAF kinase or C-RAF kinase activity, and

(b) treating the patient with an effective RAF kinase inhibiting amount of a RAF inhibiting compound as claimed in any one of claims 1 to 40 if the melanoma tissue is found to overexpress B-RAF kinase or C-RAF kinase activity.

75. A method of treating melanoma, which method comprises

(a) testing melanoma tissue from a patient and determining whether the melanoma tissue expresses mutant B-RAF kinase or C-RAF kinase activity, and

(b) treating the patient with an effective RAF kinase inhibiting amount of a RAF inhibiting compound as claimed in any one of claims 1 to 40 if the melanoma tissue is found to express mutant B-RAF kinase.

76. A method of treating a disease characterized by an activated mutant B-RAF kinase, which method comprises detecting a mutation in the B-RAF kinase gene or protein in a tissue sample from a patient and treating the patient with an effective B-RAF kinase inhibiting amount of a compound as claimed in any of claims 1 to 40.

77. A method of treating a patient having a disease characterized by excessive signaling through the MAP kinase signaling pathway, which comprises administering to the patient an effective RAF kinase inhibiting amount of a compound as claimed in any one of claims 1 to 40.

78. A process for the preparation of a compound of the formula

which process comprises the following reaction scheme:

where step 2 is optional and where carried out T' is a precursor of T₁ and R^{1 '} is a precursor of R¹ or is R¹ and X, R¹, R², T and P are as defined in claim 1.

79. A process as claimed in claim 78 wherein X is NH.

80. A process as claimed in claim 78 or 79 wherein R² is phenyl.

81. A process as claimed in claim 78, 79 or 80 wherein p is 1.

82. A process as claimed in any one of claims 78 to 81 wherein R^1' is OH.

83. A process as claimed in any one of claims 78 to 82 wherein T represents SO₂-G where G represents R⁸, NHR⁸ or NR¹⁰ and R⁸ and R¹⁰ are as defined in claim 1.

84. A process as claimed in any one of claims 78 to 83 wherein T represents O-R⁹ where R⁹ is as defined in claim 1.

85. A process as claimed in claim 84 wherein X-R²-(T)_P represents