AU2003205217A1 - Substituted quinazolin-4-ylamine analogues as modulators of capsaicin - Google Patents

Description

SUBSTITUTED QUiNAZOLlN-4-YLAMINE ANALOGUES

FIELD OF THE INVENTION

This invention relates generally to substituted quinazolin-4-ylamine analogues that are modulators of capsaicin receptors, and to the use of such compounds for treating conditions related to capsaicin receptor activation. The invention further relates to the use such compounds as probes for the detection and localization of capsaicin receptors.

BACKGROUND OF THE INVENTION Pain perception, or nociception, is mediated by the peripheral terminals of a group of specialized sensory neurons, termed "nociceptors." A wide variety of physical and chemical stimuli induce activation of such neurons in mammals, leading to recognition of a potentially harmful stimulus. Inappropriate or excessive activation of nociceptors, however, can result in debilitating acute or chronic pain. Neuropathic pain involves pain signal transmission in the absence of stimulus, and typically results from damage to the nervous system. In most instances, such pain is thought to occur because of sensitization in the peripheral and central nervous systems following initial damage to the peripheral system (e.g., via direct injury or systemic disease). Neuropathic pain is typically burning, shooting and unrelenting in its intensity and can sometimes be more debilitating that the initial injury or disease process that induced it.

Existing treatments for neuropathic pain are largely ineffective. Opiates, such as morphine, are potent analgesics, but their usefulness is limited because of adverse side effects, such as physical addictiveness and withdrawal properties, as well as respiratory depression, mood changes, and decreased intestinal motility with concomitant constipation, nausea, vomiting, and alterations in the endocrine and autonomic nervous systems, hi addition, neuropathic pain is frequently non-responsive or only partially responsive to conventional opioid analgesic regimens. Treatments employing the N-methyl-D-aspartate antagonist ketamine or the alpha(2)-adrenergic agonist clonidine can reduce acute or chronic pain, and permit a reduction in opioid consumption, but these agents are often poorly tolerated due to side effects.

Topical treatment with capsaicin has been used to treat chronic and acute pain, including neuropathic pain. Capsaicin is a pungent substance derived from the plants of the Solanaceae family (which includes hot chili peppers) and appears to act selectively on the small diameter afferent nerve fibers (A-delta and C fibers) that are believed to mediate pain. The response to capsaicin is characterized by persistent activation of nociceptors in peripheral tissues, followed by eventual desensitization of peripheral nociceptors to one or more stimuli. From studies in animals, capsaicm appears to trigger C fiber membrane depolarization by opening cation selective channels for calcium and sodium. Capsaicin responses in isolated sensory neurons show dose-dependence.

Such responses are also evoked by stractural analogues of capsaicin that share a common vanilloid moiety. One such analogue is resiniferatoxin (RTX), a natural product of Euphorbia plants. The term vanilloid receptor (VR) was coined to describe the neuronal membrane recognition site for capsaicin and such related irritant compounds. The capsaicin response is competitively inhibited (and thereby antagonized) by another capsaicin analog, capsazepine, and is also inhibited by the non-selective cation channel blocker ruthenium red. These antagonists bind to VR with no more than moderate affinity (typically with Kj values of no lower than 140 μM).

Recently, rat and human receptors for capsaicin were cloned from dorsal root ganglion cells. Such receptors have also been referred to as VRl, and the terms "VRl" and "capsaicm receptor" are used interchangeably herein to refer to rat and/or human receptors of this type, as well as mammalian homologs. The role of VRl in pain sensation has been confirmed using mice lacking this receptor, which exhibit no vanilloid-evoked pain behavior, and impaired responses to heat and inflammation. The capsaicin receptor is a nonselective cation channel with a threshold for opening that is lowered in response to elevated temperatures, low pH, and capsaicin receptor agonists. For example, the channel usually opens at temperatures higher than about 45°C. Opening of the capsaicin receptor channel is generally followed by the release of inflammatory peptides from neurons expressing the receptor and other nearby neurons, increasing the pain response. After initial activation by capsaicin, the capsaicin receptor undergoes a rapid desensitization via phosphorylation by cAMP-dependent protein kinase.

Because of their ability to thus desensitize nociceptors in peripheral tissues, VRl agonist vanilloid compounds have been used as topical anesthetics. However, agonist application may itself cause burning pain, which limits this therapeutic use.

Thus, compounds that interact with VRl but do not elicit the initial painful sensation of VRl agonist vanilloid compounds, are desirable for the treatment of chronic and acute pain, including neuropathic pain. Antagonists of this receptor are particularly desirable for the treatment of pain, as well as conditions such as tear gas exposure, itch and urinary incontinence. The present invention fulfills this need, and provides further related advantages. SUMMARY OF THE INVENTION

The present invention provides capsaicin receptor modulators that modulate, preferably inhibit, capsaicin receptor activation. Within certain aspects, compounds provided herein are characterized by the formula:

Formula I or a pharmaceutically acceptable salt thereof.

Within Formula I, V and X are each independently N or CRι, with the proviso that at least one of V and X is N; U is N or CR₂, with the proviso that if V and X are N, then U is CR₂. W, Y and Z are each independently N or CRi. R_\ is independently selected at each occurrence from hydrogen, halogen, hydroxy, cyano, amino, C_.-Csalkyl, haloCι-C₈alkyl, Ci-Csalkoxy, haloCι-C₈alkoxy and mono- and di-(C₁-C₈alkyl)amino.

R₂ is: (i) hydrogen, halogen, cyano or -COOH; (ii) C₂-C₈alkoxycarbonyl, CrCsalkanoyl, C₂-C₈alkanone, -Csalkanoyloxy, -Cscarbonate or CrCscarbamate, each of which is unsubstituted or substituted with from 1 to 9 substituents independently selected from R ; or (iii) a group ofthe formula -R_c-M-A-R_y, wherein: R_e is C₀-C₃alkyl; M is a bond, N(R_Z), O, S, SO₂, - C(=O)_pN(R_z), N(R_z)C(=O)_p, SO₂N(R_z), or N(R_z)SO₂, wherein p is 0 or 1; A is a bond or - C₈alkyl optionally substituted with from 1 to 3 substituents independently chosen from R ; and R_y and Rz are independently (a) hydrogen, d-Csalkyl, C₂-C₈alkenyl, C₂-C₈alkynyl, C₆- C₁₀arylCι-C₈alkyl, C₂-C₈alkyl ether, C alkoxy, a 4- to 10-membered carbocycle or heterocycle, or joined to R_e to form a 4- to 10-membered carbocycle or heterocycle, wherein each R_y and R_z is independently unsubstituted or substituted with from 1 to 9 substituents independently selected from R ; or (b) joined to form a 4- to 10-membered heterocycle that is unsubstituted or substituted with from 1 to 9 substituents independently selected from R_b.

R_b is independently chosen at each occurrence from hydroxy, halogen, amino, aminocarbonyl, amido, cyano, nitro, oxo, CrCsalkyl, -Csalkoxy, CrCsalkylthio, Cι-C₈alkyl ether, hydroxyC Csalkyl, haloC Csalkyl, phenyl, phenylC -C_.alkyi), mono-and di-(C₁- C₆alkyl)amino, (SO^C Csal yl, 5- to 7-membered heterocycle and (5- to 7-membered heterocycle)(C₁-C₈alkyl).

Ar₁ and Ar₂ are independently selected from 5- to 10-membered aromatic carbocycles and heterocycles, each of which is unsubstituted or substituted with from 1 to 3 substituents independently selected from groups of the formula LR_a. L is independently selected at each occurrence from a bond, -O-, -C(=O)-, -OC(=O)-, -C(=O)O-, -O-C(=0)O-, -S(O)_m-, -NR_X-, -C(=O)NHR_x-, -NHR_xC(=O)-, -NR_xS(O)_m-, -S(O)_mNR_x- and -N[S(O)_mR_x]S(O)_m-; wherem m is independently selected at each occurrence from 0, 1 and 2; and R_x is independently selected at each occurrence from hydrogen and C_\- C₈alkyl. R_a is independently selected at each occurrence from: (i) hydrogen, halogen, cyano and nitro; and (ii) Cι-C₈alkyl, C₂-C₈alkenyl, C -C₈alkynyl, C₂-C₈alkyl ether, 3- to 10-membered heterocycles, mono- and di-(C₁-C₈alkyl)amino and (3- to 10-membered heterocycle)C₁-C₆alkyl, each of which is optionally substituted with from 1 to 9 substituents independently selected from R_b. Within certain compounds of Formula I, Ar₂ is a 5- to 7-membered aromatic heterocycle, optionally substituted as described above. Within further aspects, compounds provided herein are characterized by the formula:

Formula II or a pharmaceutically acceptable salt thereof. Within Formula II, U, V, W, X, Y and Z are as described above. A and Ar₂ of Formula II are each independently chosen from phenyl and 5- and 6-membered aromatic heterocycles, optionally substituted with from 1 to 3 substituents independently selected from groups ofthe formula LRg, as described above. R₇ of Formula II is C C_.alkyl, C₂-C₈alkenyl, C₂-C₈alkynyl, mono- or di-(C₁-C₈alkyl)amino or a 3- to 10-membered heterocycle, each of which is optionally substituted with from 1 to 5 substituents independently selected from hydroxy, halogen, Cι-C₆alkyl, Ci-Csalkoxy, C₂-C₈alkyl ether, haloCi-Csalkyl and haloCι-C₈alkoxy. Within still further aspects, compounds provided herein are substituted 2-aminoalkyl- quinazoline-4ylamine analogues of Formula III:

Formula III or a pharmaceutically acceptable salt or prodrug thereof, wherein:

V, X, W, Y and Z are as described above; Ar, and Ar₂ are independently selected from phenyl and 5- to 7-membered aromatic heterocycles, each of which is unsubstituted or substituted with from 1 to 3 substituents independently selected from groups ofthe formula LR_a as described above; R and R₄ are:

(i) each independently selected from:

(a) hydrogen;

(b) C_.-Csalkyl, C₂-C₈alkenyl, C₂-C₈alkynyl, Ci-Csalkoxy, C₃-C₈alkanone, C₂- C₈alkanoyl, C₂-C₈alkyl ether, C₆-C₁₀arylCo-C₈alkyl, 5- to 10-membered heterocycleC₀-C₈alkyl and -(SO_)Cι-C₈alkyL each of which is optionally substituted with from 1 to 9 substituents independently selected from R_b; and

(c) groups that are joined to an R₅ or R₆ to form a 4- to 10-membered heterocyclic group that is unsubstituted or substituted with from 1 to 6 substituents independently selected from R_b; or

(ii) joined to form, with the N to which they are bound, a 4- to 10-membered heterocyclic group that is unsubstituted or substituted with from 1 to 6 substituents independently selected from R_b, Cι-C₈alkanoyl, 4- to 7-membered heterocycloalkylCo-C₄alkyl, and mono- and di-(C₁-C₆alkyl)aminoC₁-C₆alkyl; R₅ and Re are, independently at each occurrence: (i) each independently selected from:

(a) hydrogen or hydroxy;

(b) Cι-C₈alkyl, unsubstituted or substituted with 1 or 2 substituents independently selected from R_b; and (c) groups that are joined to R₃ or R to form a 4- to 10-membered heterocyclic group that is unsubstituted or substituted with from 1 to 6 substituents independently selected from R_b; (ii) taken together to form a keto group; or

(iii) joined to form a 3- to 7-membered carbocychc or heterocyclic ring, unsubstituted or substituted with from 1 to 4 substituents selected from R_b;

R is as described above; and n is 1, 2 or 3.

Within certain additional aspects, the compounds provided herein are substituted 2- hydroxyalkyl-quinazoline-4ylamine analogues of Formula IV:

Formula TN or a pharmaceutically acceptable salt or prodrug thereof, wherein: N, X, W, Y and Z are as described above;

Ar_ and Ar₂ are independently selected from phenyl and 5- to 7-membered aromatic heterocycles, each of which is unsubstituted or substituted with from 1 to 3 substituents independently selected from groups ofthe formula LR_a as described above; R is selected from:

(i) hydrogen;

(ii) Ci-Csalkyl, C₂-C₈alkenyl, C₂-C₈alkynyl, C₂-C₈alkyl ether, C₆-C₁₀arylC₀-C₈alkyl, and 5- to 10-membered heterocycleC₀-C₈alkyl, each of which is optionally substituted with from 1 to 9 substituents independently selected from R_b; and (iii) groups that are joined to an R₅ or R$ to form a 4- to 10-membered heterocyclic group that is unsubstituted or substituted with from 1 to 6 substituents independently selected from

R ; R₅ and R₆ are, independently at each occurrence: (i) each independently selected from: (a) hydrogen or hydroxy;

(b) Cι-C₈alkyl, unsubstituted or substituted with 1 or 2 substituents independently selected from R_b; and

(c) groups that are joined to R₃ to form a 5- to 10-membered heterocyclic group that is unsubstituted or substituted with from 1 to 6 substituents independently selected

(ii) joined to form a 3- to 7-membered carbocychc or heterocyclic ring, unsubstituted or substituted with from 1 to 4 substituents selected from R_b; R_b is as described above; and n is 1, 2 or 3. Within certain aspects, compounds as described herein exhibit a K; of no greater than 1 micromolar, 100 nanomolar, 10 nanomolar or 1 nanomolar in a capsaicin receptor binding assay and/or have an IC₅₀ value of no greater than 1 micromolar, 100 nanomolar, 10 nanomolar or 1 nanomolar in an assay for determination of capsaicin receptor antagonist activity. Preferred compounds generally include those with higher potency (i.e., lower Kj or lower IC₅₀). Within certain aspects, compounds as described herein are labeled with a detectable marker (e.g., radiolabeled or fluorescein conjugated).

The present invention further provides, within other aspects, pharmaceutical compositions comprising at least one compound or salt as described herein in combination with a physiologically acceptable carrier or excipient. Within further aspects, methods are provided for reducing calcium conductance of a cellular capsaicin receptor, comprising contacting a cell (e.g., neuronal) expressing a capsaicin receptor with an effective amount of at least one compound or salt as described herein. Such contact may occur in vivo or in vitro. Methods are further provided, within other aspects, for inhibiting binding of vanilloid ligand to a capsaicin receptor. Within certain such aspects, the inhibition takes place in vitro. Such methods comprise contacting a capsaicin receptor with at least one compound or salt as described above, under conditions and in an amount sufficient to detectably inhibit vanilloid ligand binding to the capsaicin receptor. Within other such aspects, the capsaicin receptor is in a patient. Such methods comprise contacting cells expressing a capsaicin receptor in a patient with at least one compound or salt as described herein in an amount sufficient to detectably inhibit vanilloid ligand binding to cells expressing a cloned capsaicin receptor in vitro, and thereby inhibiting binding of vanilloid ligand to the capsaicin receptor in the patient.

The present invention provides, within further aspects, methods for treating a condition responsive to capsaicin receptor modulation in a patient, comprising administering to the patient a capsaicin receptor modulatory effective amount of at least one compound or salt as described herein, and thereby alleviating the condition in the patient.

Within other aspects, methods are provided for treating pain in a patient, comprising administering to a patient suffering from pain a capsaicin receptor modulatory amount of at least one compound or salt as described herein, and thereby alleviating pain in the patient.

Methods are further provided, within other aspects, for treating itch, urinary incontinence, cough and/or hiccup in a patient, comprising administering to a patient suffering from one or more ofthe foregoing conditions a capsaicin receptor modulatory amount of at least one compound or salt as described herein, and thereby alleviating the condition(s) in the patient. The present invention further provides methods for promoting weight loss in an obese patient, comprising administering to an obese patient a capsaicin receptor modulatory amount of at least one compound or salt as described herein, and thereby promoting weight loss in the patient.

Within further aspects, the present invention provides methods for determining the presence or absence of capsaicin receptor in a sample, comprising the steps of: (a) contacting a sample with a compound as described herein under conditions that permit binding of the compound to capsaicin receptor; and (b) detecting a level of the compound bound to capsaicin receptor. The present invention further provides packaged pharmaceutical preparation, comprising: (a) a pharmaceutical composition as described herein in a container; and (b) instructions for using the composition to treat one or more conditions responsive to capsaicin receptor modulation, such as pain, itch, urinary incontinence, cough, hiccup, and/or obesity. In yet another aspect, the present invention provides methods of preparing the compounds disclosed herein, including the intermediates.

These and other aspects ofthe present invention will become apparent upon reference to the following detailed description.

DETAILED DESCRIPTION

As noted above, the present invention provides capsaicin receptor modulators comprising substituted quinazolin-4-ylamine analogues. Such modulators may be used in vitro or in vivo, to modulate (preferably inhibit) capsaicin receptor activity in a variety of contexts. Certain compounds of Formula I include those in which: V, X, W, Y and Z are each independently N or CR_, with the proviso that at least one of V and X is N; U is N or CR₂, with the proviso that if V and X are N, then U is CR₂;

Ri is independently selected at each occurrence from hydrogen, halogen, hydroxy, amino, C_- C₈alkyl, haloCι-C₈alkyl, Cι-C₈alkoxy, halo -Csalkoxy and mono- and di-(C_- C₈alkyl)amino;

R₂ is: (i) hydrogen, halogen, cyano or -COOH;

(ii) C_-C₈alkanoyl, C₂-C₈alkanone, or C Cscarbamate, each of which is unsubstituted or substituted with from 1 to 9 substituents independently selected from R_b; or (iii) a group ofthe formula -R_c-M-A-R_y, wherein: _e is C₀-C₃alkyl;

M is a bond, N(R_Z), O, S, SO₂, (C=O)_pN(R_z), N(R_z)(C=O)_p, SO₂N(R_z), or

N(R_z)SO₂, wherein p is 0 or 1; A is a bond or Cι-C₈alkyl, optionally substituted with from 1 to 3 substituents independently selected from R_b; and R_y and R_z, if present, are:

(a) independently hydrogen, Ci-Csalkyl, C₂-C₈alkenyl, C₂-C₈alkynyl, C₆- CioarylCi-Csalkyl, C₂-C₈alkyl ether, Cι-C₈alkoxy, a 4- to 10-membered carbocycle or heterocycle, or joined to R_e to form a 4- to 10-membered carbocycle or heterocycle, wherein each R_y and R_z is independently unsubstituted or substituted with from 1 to 9 substituents independently selected from R ; or (b) joined to form a 4- to 10-membered carbocycle or heterocycle that is unsubstituted or substituted with from 1 to 9 substituents independently selected from R ;

Ar₂ is a 5- to 7-membered aromatic heterocycle, optionally substituted with from 1 to 3 substituents independently selected from groups ofthe formula LR_a; Aτ_\ is a 5- to 10-membered aromatic carbocycle or heterocycle, optionally substituted with from 1 to 3 substituents independently selected from groups ofthe formula LR_a; L is independently selected at each occurrence from a bond, -O-, -C(=O)-, -OC(=O)-, -C(=O)O-, -0-C(=0)O-, -S(O)_m-, -NR_X-, -C(=O)NHR_x-, -NHR_xC(=O)-, -NR_xS(O)_m-, - S(O)_mNR_x- and -N[S(O)_mR_x]S(O)_m-; wherein m is independently selected at each occurrence from 0, 1 and 2; and R_x is independently selected at each occurrence from hydrogen and Cι-C₈alkyl; R_a is independently selected at each occurrence from: (i) hydrogen, halogen, cyano and nitro; and

(ii) Ci-Csalkyl, C₂-C₈alkenyl, C₂-C₈alkynyl, C₂-C₈alkyl ether, 3- to 10-membered heterocycles, mono- and di-(C₁-C₈alkyl)amino and (3- to 10-membered heterocycle)C₁- C₆alkyl, each of which is unsubstituted or substituted with from 1 to 9 substituents independently selected from Rb; and

R_b is independently chosen at each occurrence from hydroxy, halogen, amino, aminocarbonyl, amido, cyano, nitro, Cι-C₈alkyl, CrCsalkoxy, C Csalkylthio, C_-C₈alkyl ether, hydroxyCi-

C₈alkyl, halo -Csalkyl, phenyl, pheny^C Csalkyl), mono-and di-(C₁-C₆alkyl)amino,

(SO₂)Cι-C₈alkyl, 5- to 7-membered heterocycle and (5- to 7-membered heterocycle)(C₁- C₈alkyl).

Such compounds are referred to herein as compounds of Formula lb.

Within certain compounds of Formula lb, Ar₂ is selected from pyridyl, pyridazinyl, pyrimidinyl, pyrazinyl, pyrrolyl, imidazolyl, pyrazolyl, oxazolyl, isoxazolyl, thiazolyl, isothiazolyl and thiadiazolyl, each of which is unsubstituted or substituted with 1 or 2 substituents selected from halogen, cyano, Cι-C₆alkyl, haloCι-C₆alkyl, hydroxyCi-Cβalkyl, C_- C_.alkyl ether, C_-C₆alkanoyl, amino, mono- and di-(C₁-C₆alkyl)amino. In certain embodiments, Ar₂ is pyridyl, isoxazolyl, thiadiazolyl or pyrazolyl, each of which is imsubstituted or substituted with halogen, C₁-C₄alkyl orhaloC₁-C₄alkyl. In certain embodiments, Ar_ is phenyl or pyridyl, optionally substituted with halogen, C_-C₆alkyl, haloCι-C₆alkyl, C_-C₆alkoxy or haloCi-Cβalkoxy.

Within certain compounds of Formula lb, U is CR₂. In certain embodiments, R₂ is: (i) hydrogen or halogen; or (ii) C.-Cβalkyl, -(CH₂)_nNH₂, -(CH₂)_nNH(C₁-C₈alkyl), -(CH₂)„N(Cι-C₈alkyl)2,

-(CH₂)_n(5- to 8-membered heterocycloalkyl), -(CH₂)_nOH or -(CH₂)_nO(C₁-C₈alkyl), each of which is unsubstituted or substituted with from 1 to 4 substituents independently chosen from halogen, cyano, hydroxy, amino, mono- and di-(C₁-C₆alkyl)amino, CrCβalkyl, and haloCr C₆alkyl. In another aspect, the invention provides compounds of Formula lib, i.e., compounds of

Formula II, wherein N, X, W, Y and Z are each independently Ν or CRi, with the proviso that at least one of N and

X is Ν; U is Ν or CR₂, with the proviso that if N and X are Ν, then U is CR₂; Ri is independently selected at each occurrence from hydrogen, halogen, hydroxy, amino, C_- C₈alkyl, haloC_-C₈alkyl, CrCsalkoxy, haloC Csalkoxy and mono- and di-(Cr C₈alkyl)amino; R₂ is: (i) hydrogen, halogen, cyano or -COOH;

(ii) -Csalkanoyl, C₂-C₈alkanone, or Ci-Cscarbamate, each of which is unsubstituted or substituted with from 1 to 9 substituents independently selected from R_b; or

(iii) a group ofthe formula -Rς-M-A-R_y, wherein: R_e is Co-C₃alkyl;

M is a bond, Ν(R_), O, S, SO₂, (C=O)_pN(R_z), N(R_z)(C=O)_p, SO₂N(R₂), or N(R_z)SO₂, wherein p is 0 or 1; A is a bond or Cι-C₈aikyl, optionally substituted with from 1 to 3 substituents independently selected from R_b; and R_y and R_z are:

(a) independently hydrogen, -Csalkyl, C₂-C₈alkenyl, C₂-C₈alkynyl, C₆-

C_₀arylCι-C₈alkyl, C₂-C₈alkyl ether, -Csalkoxy, a 4- to 10-membered carbocycle or heterocycle, or joined to R_e to form a 4- to 10-membered carbocycle or heterocycle, wherein each R_y and R_z is independently unsubstituted or substituted with from 1 to 9 substituents independently selected from R_b; or (b) joined to form a 4- to 10-membered carbocycle or heterocycle that is unsubstituted or substituted with from 1 to 9 substituents independently selected from R_b; Ar_ and Ar₂ are independently chosen from phenyl and 5- and 6-membered aromatic heterocycles, optionally substituted with from 1 to 3 substituents independently selected from groups ofthe formula LR_a; L is independently selected at each occurrence from a bond, -O-, -C(=O)-, -OC(=O)-, -C(=O)O-, -OC(=0)O-, -S(O)_m-, -NR_X-, -C(=O)NHR_X-, -NHR_xC(=O)-, -NR_xS(O)_m-, - S(O)_mNR_x- and -N[S(O)_mR_x]S(O)_m-; wherein m is independently selected at each occurrence firom 0, 1 and 2; and R_x is independently selected at each occurrence from hydrogen and Ci-C₈alkyl; R_a is independently selected at each occurrence from: (i) hydrogen, halogen, cyano and nitro; and

(ii) Cι-C₈alkyl, C₂-C₈alkenyl, C₂-C₈alkynyl, C₂-C₈alkyl ether, 3- to 10-membered heterocycles, mono- and di-(C₁-C₈alkyl)amino and (3- to 10-membered heterocycle)C₁-

C₆alkyl, each of which is unsubstituted or substituted with from 1 to 9 substituents independently selected from R ; R_b is independently chosen at each occurrence from hydroxy, halogen, amino, aminocarbonyl, amido, cyano, nitro, -Csalkyl, -Csalkoxy, CrCsalkylthio, - alkyl ether, hydroxyCr C₈alkyl, haloCrCsalkyl, phenyl, mono-and di-(C₁-C₆alkyl)amino,

(S0₂)C₁-C₈alkyl, 5- to 7-membered heterocycle and (5- to 7-membered heterocycle)(Cι- C₈alkyl); and R₇ is Ci-Cβalkyl, C₂-C₈alkenyl, C₂-C₈alkynyl, mono- or di(C₁-C₈alkyl)amino or a 3- to 10- membered heterocycle, each of which is optionally substituted with from 1 to 5 substituents independently selected from hydroxy, halogen, Ci-Cβalkyl, Cι-C₈alkoxy, C₂-C₈alkyl ether, haloCι-C₈alkyl and haloCrCsalkoxy. i certain compounds of Formula lib Ar₂ is phenyl or pyridyl, each of which is optionally substituted with 1 or 2 substituents selected from halogen, cyano, Ci-Cealkyl and haloCi-C_δalkyl. In certain compounds Ar is phenyl, optionally substituted with halogen, C_- C₄alkyl or haloC_.- alkyl.

In certain compounds of Formula lib Ari is phenyl or pyridyl, optionally substituted with halogen, Cι-C₆alkyl, haloC C_όalkyl, -Cealkoxy or haloCi-Cealkoxy. In certain embodiments, Ari and Ar₂ are each pyridyl, substituted with 1 substituent independently chosen from halogen, CrC₄alkyl, - haloalkyl, and C₁-C₄alkoxy. h certain compounds of Formula lib U is CR₂. In certain embodiments, R₂ is (i) hydrogen or halogen; or

(ii) C.-C₆alkyl, -(CH₂)_nNH₂, -(CH₂)„NH(C₁-C₈alkyl), -(CH2)„N(C₁-C₈alkyl)₂,

-(CH₂)_n(5- to 8-membered heterocycloalkyl), -(CH₂)_nOH or -(CH₂)nO(C₁-C₈alkyl), each of which is unsubstituted or substituted with from 1 to 4 substituents independently chosen from halogen, cyano, hydroxy, amino, mono- and di-(C₁-C₆alkyl)amino, C_-

C₆alkyl and haloC_-C₆alkyl. hi certain embodiments, X and V are each N. h certain compounds of Formula lib R comprises a nitrogen atom directly bonded to the SO₂. hi certain embodiments, R₇ is amino, mono-or di(C₁-C₆alkyl)amino, morpholinyl, piperidinyl, piperazinyl or pyrrolidinyl, each of which is unsubstituted or substituted with from 1 to 3 substituents independently chosen from halogen, Ci-C_όalkyl and haloCι-C₆alkyl. In other compounds of Formula lib, R₇ is Ci-C_δalkyl, haloCi-C₆alkyl, morpholinyl, piperidinyl, piperazinyl or pyrrolidinyl, optionally substituted with from 1 to 5 substituents independently selected from C_-C₆alkyl, -Cealkoxy, Cι-C₆alkyl ether, haloCrCβalkyl andhalo -C_όalkoxy. hi another aspect, the invention provides compounds of Formula Illb, i.e., compounds wherein:

N, X, W, Y and Z are each independently Ν or CR_, with the proviso that at least one of N and

X is Ν; Ri is independently selected at each occurrence from hydrogen, halogen, hydroxy, cyano, amino, -Csalkyl, haloCι-C₈alkyl, CrC₈alkoxy, halo -Csalkoxy and mono-and di-(Cι-

C₆alkyl) amino; Ar₁ and Ar₂ are independently selected from phenyl and 5- to 7-membered aromatic heterocycles, each of which is unsubstituted or substituted with from 1 to 3 substituents independently selected from groups ofthe formula LR_a; L is independently selected at each occurrence from a bond, -O-, -C(=O)-, -OC(=O)-, -C(=O)O-, -O-C(=O)O-, -S(O)_m-, -ΝR_X-, -C(=O)NHR_x-, -NHR_xC(=O)-, -NR_xS(O)_m-, - S(O)_mNR_x- and -N[S(O)_mR_x]S(O)_m-; wherein m is independently selected at each occurrence from 0, 1 and 2; and R_x is independently selected at each occurrence from hydrogen and Cι-C₈alkyl; R_a is independently selected at each occurrence from: (i) hydrogen, halogen, cyano and nitro; and

(ii) CrCsalkyl, C₂-C₈alkenyl, C₂-C₈alkynyl, C₂-C₈alkyl ether, 3- to 10-membered heterocycles, and (3- to 10-membered heterocycle)C₁-C₆alkyl, each of which is unsubstituted or substituted with from 1 to 9 substituents independently selected from R ; R₃ and P_ι are:

(i) each independently selected from:

(a) hydrogen;

(b) d-Cgalkyl, C₂-C₈alkenyl, C₂-C₈alkynyl, Ci-Csalkoxy, C₃-C₈alkanone, C₂- C₈alkanoyl, C₂-C₈alkyl ether, C₆-C₁₀arylCo-C₈alkyl, 5- to 10-membered heterocycleC₀-C₈alkyl and -(SO₂)C₁-C₈alkyl, each of which is optionally substituted with from 1 to 9 substituents independently selected from R_b; and

(c) groups that are joined to an R₅ or R₆ to form a 4- to 10-membered heterocyclic group that is unsubstituted or substituted with from 1 to 6 substituents independently selected from R ; or

(ii) joined to form, with the N to which they are bound, a 4- to 10-membered heterocyclic group that is unsubstituted or substituted with from 1 to 6 substituents independently selected from R_b, Ci-Csalkanoyl, 4- to 7-membered heterocycloalkylCo-C₄alkyl, and mono- and di-(C₁-C₆alkyl)aminoC₁-C₆alkyl; R₅ and Rg are, independently at each occurrence: (i) each independently selected from:

(a) hydrogen or hydroxy;

(b) Ci-C₈alkyl, unsubstituted or substituted with 1 or 2 substituents independently selected from R ; and (c) groups that are joined to R₃ or R₄ to form a 4- to 10-membered heterocyclic group that is unsubstituted or substituted with from 1 to 6 substituents independently selected from R_b; (ii) taken together to form a keto group; or

(iii) joined to form a 3- to 7-membered carbocychc or heterocyclic ring, unsubstituted or substituted with from 1 to 4 substituents selected from R_b;

R_b is independently chosen at each occurrence from hydroxy, halogen, amino, aminocarbonyl, amido, cyano, nitro, Cι-C₈alkyl, C_-C₈alkoxy, Ci-Csalkylthio, C_-C₈alkyl ether, hydroxy -

C₈alkyl, haloCι-C₈alkyl, phenyl, mono-and di-(C₁-C₆alkyl)amino,

(SO₂)Ci-C₈alkyl, 5- to 7-membered heterocycle and (5- to 7-membered heterocycle) (C_- C_.alkyl); and n is 1, 2 or 3.

In certain compounds of Formula Illb, Ar_. and Ar₂ are independently selected from phenyl and 6-membered aromatic heterocycles, each of which is substituted with 0, 1 or 2 substituents. hi certain embodiments, (i) Ar_t is phenyl or pyridyl, each of which is unsubstituted or substituted with 1 or 2 substituents selected from halogen, hydroxy, cyano, amino, nitro, mono- and di-(Cι-C₆alkyl)amino, Cι-C₆alkyl, haloCι-C₆alkyl, d-Cβalkoxy and halod- C₆alkoxy; and (ii) Ar₂ is phenyl or pyridyl, each of which is unsubstituted or substituted with 1 or 2 substituents independently selected from halogen, hydroxy, cyano, amino, mono- and di- (C_-C₆a_-yl)amino, Ci-C₆alkyl, haloCι-C₆alkyl, C_-C₆alkoxy, haloC_-C₆alkoxy, C₂-C6alkyl ether, -C.alkanoyl, -(SO₂)R₂,

-NR_xS(O)_m-, and -N(S(O_m)₂; wherein m is 1 or 2, R_x is hydrogen or Cι-C₆alkyl, and R₂ is C_- C_.alkyl, haloCι-C₆alkyl, amino, mono- or di-(CrC₆alkyl)amino or a 5- to 10-membered, N- linked heterocyclic group, each of which R₂ is optionally substituted with R_b. For example, in some embodiments, (i) Ari is pyridyl, unsubstituted or substituted with halogen, C_-

C alkyl or haloCi-C alkyl; and (ii) Ar₂ is phenyl or pyridyl, each of which is unsubstituted or substituted with halogen, cyano, C₁-C₄alkyl, haloC₁-C₄alkyl, C₂-C alkyl ether, C₁-C alkanoyl or -(SO₂)R_a, wherein R_a is C₁-C alkyl or haloC₁-C₄alkyl. Certain such compounds are those in which (i) Ar_. is pyridin-2-yl, 3-methyl-pyridin-2-yl, 3-trifluoromethyl-pyridin-2-yl or 3-halo- pyridin-2-yl; and (ii) Ar₂ is phenyl, 2-pyridyl or 3-pyridyl, each of which is substituted at the 4- position with trifluoromethanesulfonyl, propanesulfonyl, propane-2-sulfonyl, t-butyl, trifluoromethyl or 2,2,2-trifluoro-l -methyl-ethyl.

In certain compounds of Formula Illb, R₃ and R₄ are each independently: (i) hydrogen; or (ii) Ci-Csalkyl, C₂-C₈alkenyl, phenylC₀-C₄alkyl, indanylC₀-C₄alkyl, 5- to 6-membered heteroarylC₀-C₄alkyl, or 4- to 7-membered heterocycloalkylC₀-C₄alkyl, each of which is unsubstituted or substituted with from 1 to 4 substituents independently selected firom hydroxy, halogen, amino, Cι-C₆alkyl, haloCi-Cealkyl, C_-C₆alkoxy and haloC Cβalkoxy. In certain embodiments, R₃ and _t are each independently (i) hydrogen; or (ii) Ci-C₆alkyl, C₂-C₆alkenyl, 5- to 7-membered heterocycloC₀-C₄alkyl, C₂-C₆alkyl ether, indanyl, benzyl, 1-phenyl-ethyl, 1- phenyl-propyl and 2-phenyl-ethyl, each of which is unsubstituted or substituted with from 1 to 3 substituents independently selected firom hydroxy, halogen and C₁-C₄alkyl. For example, one of R₃ and may be pyridylCo-C alkyl, pyrimidylC₀-C₄alkyl, imidazolylCo-C alkyl or tetrazolylC₀-C₄alkyl, each of which is substituted with 0, 1 or 2 substituents.

In certain compounds of Formula Illb, R₃ and R₄ are joined to form a 5 to 10-membered heterocyclic group that is substituted with from 0 to 4 substituents. hi certain embodiments, the heterocyclic group is substituted with at least one substituent selected from hydroxy, halogen, C_-C₄alkyl, haloCrG_talkyl, C₁-C alkoxy, haloCι-C₄alkoxy, C₁-C₄alkanoyl, and aminocarbonyl. In certain embodiments, the heterocyclic group comprises an aromatic ring. One heterocyclic group is 3,4-dihydro-lH-isoquinolin-2-yl, substituted with 0, 1 or 2 substituents. hi other embodiments, the heterocyclic group is a 5- to 10-membered heterocycloalkyl, substituted with firom 0 to 4 substituents. For example, the heterocycloalkyl may be piperadinyl, piperazinyl, pyrrolidinyl, azepanyl, azocinyl, decahydroquinolinyl or l,4-dioxa-8-aza-spiro[4.5]dec-8-yl, each of which is unsubstituted or substituted with from 1 to 4 substituents independently selected from halogen, hydroxy, Cι-C alkyl, Ci-C₄alkoxy, haloCι-C₄alkyl, haloCi-C₄alkoxy, C_- C alkanoyl and Ci-C₄alkoxycarbonyl. Still further heterocyclic groups include morpholino, thiomorpholino or l,l-dioxo-thiomorpholin-4-yl, each of which is unsubstituted or substituted with from 1 to 4 substituents independently selected from halogen, hydroxy, Cι-C₄alkyl, C_- C₄alkoxy, haloCι-C₄alkyl, haloC₁-C₄alkoxy, Ci-C₄alkanoyl and C₁-C alkoxycarbonyl. In certain compounds, the heterocyclic group is substituted with from 1 to 4 substituents independently selected from methyl and ethyl.

In certain compounds of Formula Illb, R₅ and R₆ are independently selected from hydrogen and Ci-C₆alkyl. In some embodiments, R₅ and Re are hydrogen. In certain compounds of Formula Illb, n is 1. Certain representative compounds of Formula Illb are those compounds wherein:

(i) N and X are Ν;

(ii) Ari is pyridyl, unsubstituted or substituted with halogen, Ci-C₄alkyl or haloCi-C₄alkyl; (iii)Ar2 is phenyl or pyridyl, unsubstituted or substituted with Ci-C₄alkyl, haloCi-C₄alkyl or a group ofthe formula -(SO₂)R₂, wherein R₂ is Ci-C₄alkyl or haloCi-C₄alkyl; (iv)R and _t are each independently selected from Cι-C₆alkyl, C₂-C₆alkenyl, C₂-C₆alkyl ether, 5- to 10-membered heteroarylC₀-C₄alkyl, phenylC₀-C alkyl and indanyl, each of which is substituted with 0, 1 or 2 substituents independently selected from hydroxy, halogen, Ci-C₄alkyl and haloCι-C₄alkyl; and (v) n is 1. Yet other compounds of Formula Illb are those in which:

(i) N and X are Ν;

(ii) Ari is pyridyl, unsubstituted or substituted with halogen, Ci-C₄alkyl or haloCi-C alkyl; (iii)Ar₂ is phenyl or pyridyl, unsubstituted or substituted with Ci-C₄alkyl, haloCi-C alkyl or a group ofthe formula -(SO2)R₂, wherein R₂ is Ci-C₄alkyl or haloC₁-C₄alkyl; (iv)R₃ and R are joined to form a 5- to 10-membered heterocyclic group that is unsubstituted or substituted with from 1 to 3 substituents; and (v) n is 1. In yet another aspect, the invention provides compounds of Formula TNb, i.e. compounds of Formula IN wherein:

N, X, W, Y and Z are each independently Ν or CRi, with the proviso that at least one of N and X is ; Ri is independently selected at each occurrence from hydrogen, halogen, hydroxy, cyano, amino, Cι-C₈alkyl, haloCi-C₈alkyl, Cι-C₈alkoxy, haloCi-C₈alkoxy and mono-and di-(Ci- C₆alkyl)amino; Ari and Ar₂ are independently selected from phenyl and 5- to 7-membered aromatic heterocycles, each of which is unsubstituted or substituted with from 1 to 3 substituents independently selected from groups ofthe formula LR_a;

L is independently selected at each occurrence firom a bond, -O-, -C(=O)-, -OC(=O)-,

-C(=O)O-, -0-C(=0)O-, -S(O)_m-, -ΝR_X-, -C(=O)NHR_x-, -NHR_xC(=O)-, -NR_xS(O)_m-, -

S(O)_mNR_x- and -N[S(O)_mR_x]S(O)_m-; wherein m is independently selected at each occurrence from 0, 1 and 2; and R_x is independently selected at each occurrence from hydrogen and Ci-C₈alkyl;

R_a is independently selected at each occurrence from: (i) hydrogen, halogen, cyano and nitro; and

(ii) Ci-C₈alkyl, C₂-C₈alkenyl, C₂-C₈alkynyl, C₂-C₈alkyl ether, 3- to 10-membered heterocycles, mono- and di-(Ci-C₈alkyl)amino and (3- to 10-membered heterocycle)Ci- C₆alkyl, each of which is unsubstituted or substituted with from 1 to 9 substituents independently selected from R ; R is selected from: (i) hydrogen;

(ii) Ci-C₈alkyl, C₂-C₈alkenyl, C₂-C₈alkynyl, C₂-C₈alkanone, C₂-C₈alkyl ether, C₆-Cι₀arylC₀- C₈alkyl, and 5- to 10-membered heterocycleC₀-C₈alkyl, each of which is optionally substituted with from 1 to 9 substituents independently selected firom R_b; and (iii) groups that are joined to an R₅ or R_ to form a 4- to 10-membered heterocyclic group that is unsubstituted or substituted with from 1 to 6 substituents independently selected from R_b; R₅ and Re are, independently at each occurrence: (i) each independently selected from:

(a) hydrogen or hydroxy;

(b) Ci-C₈alkyl, unsubstituted or substituted with 1 or 2 substituents independently selected from R_b; and (c) groups that are joined to R₃ to form a 4- to 10-membered heterocyclic group that is unsubstituted or substituted with firom 1 to 6 substituents independently selected (ii) joined to form a 3- to 7-membered carbocychc or heterocyclic ring, unsubstituted or substituted with from 1 to 4 substituents selected from R ;

R_b is independently chosen at each occurrence from hydroxy, halogen, amino, aminocarbonyl, amido, cyano, nitro, Cι-C₈alkyl, Cr alkoxy, Ci-C₈alkylthio, Ci-C₈alkyl ether, hydroxyCi-

C₈alkyl, haloCι-C₈alkyl, phenyl, phenyl(Ci-C₈alkyl), mono-and di-(Ci-C₆alkyl)amino,

(SO₂)Ci-C₈alkyl, 5- to 7-membered heterocycle and (5- to 7-membered heterocycle)(Ci- C_.alkyl); and n is 1, 2 or 3.

In certain compounds of Formula IVb, Ari and A_2 are independently selected from phenyl and 6-membered aromatic heterocycles, each of which is substituted with 0, 1 or 2 substituents. hi certain embodiments, (i) Ari is phenyl or pyridyl, each of which is unsubstituted or substituted with 1 or 2 substituents selected from halogen, hydroxy, cyano, amino, nitro, mono- and di-(Ci-C₆alkyl)amino, Ci-C₆alkyl, halo -Cβalkyl, Cι-C₆alkoxy and haloCi- C₆alkoxy; and (ii) Ar₂ is phenyl or pyridyl, each of which is unsubstituted or substituted with 1 or 2 substituents selected from halogen, hydroxy, cyano, amino, nitro, mono- and di-(Ci- C₆alkyl)amino, Ci-C₆alkyl, haloCi-C₆alkyl, Ci-C₆alkoxy, haloCi-C₆alkoxy, C2-C₆alkyl ether, Cι-C₆alkanoyl, -(SO2)R₂, -NR_xS(O)_m-, and -N(S(O_m)2; wherein m is 1 or 2, R_x is hydrogen or Cι-C₆alkyl, and R₂ is Ci-C₆alkyl, haloCi-C₆alkyl, amino, mono- or di-(Ci-C₆alkyl)anτino or a 5- to 10-membered, N-linked heterocyclic group, each of which R₂ is optionally substituted with R_b. hi certain such compounds, (i) Ari is pyridyl, unsubstituted or substituted with halogen, - C alkyl or haloCi-C alkyl; and (ii) Ar₂ is phenyl or pyridyl, unsubstituted or substituted with C_- C alkyl, haloCi-C alkyl or a group of the formula -(SO₂)R2, wherein R2 is Ci-C alkyl or haloCi-C alkyl. In some such compounds, (i) Ari is pyridin-2-yl, 3-methyl-pyridin-2-yl, 3- trifluoromethyl-pyridin-2-yl or 3-halo-pyridin-2-yl; and (ii) Aτ₂ is phenyl, 2-pyridyl or 3- pyridyl, each of which is substituted at the 4-position with trifluoromethanesulfonyl, propanesulfonyl, propane-2-sulfonyl, t-butyl, trifluoromethyl or 2,2,2-trifluoro-l -methyl-ethyl. In certain compounds of Formula IVb, R is: (i) hydrogen; or (ii) Ci-C₈alkyl, C -

C₈alkenyl, phenylC₀-C₄alkyl, 5- to 6-membered heteroarylCo-C alkyl, or 4- to 7-membered heterocycloalkylC₀-C₄alkyl, each of which is unsubstituted or substituted with from 1 to 4 substituents independently selected from hydroxy, halogen, amino, Ci-C₆alkyl, haloCi-C₆alkyl, Cι-C₆alkoxy and haloCi-C₆alkoxy. hi certain embodiments, R₃ is: (i) hydrogen; or (ii) C_- C₆alkyl or benzyl, each of which is unsubstituted or substituted with from 1 to 3 substituents independently selected from hydroxy, halogen and Cι-C₄alkyl.

In certain compounds of Formula IVb, each R₅ and R₆ is independently selected from hydrogen and Ci-C₆alkyl. In certain embodiments, R₅ and R_ are hydrogen. In certain compounds of Formula IVb, n is 1.

Certain representative compounds of Formula IVb are those in which: (i) V and X are N;

(ii) Ari is pyridyl, unsubstituted or substituted with halogen, Cι-C₄alkyl or haloCι-C₄alkyl; (iii) Ar₂ is phenyl or pyridyl, unsubstituted or substituted with Cι-C₄alkyl, haloCι-C₄alkyl or a group ofthe formula -(SO₂)R₂, wherein R₂ is Cι-C₄alkyl or haloCι-C alkyl;

(iv) R₃ is selected firom (a) hydrogen; and (b) Cι-C₆alkyl, C₂-C₆alkenyl and phenylC₀-C₄alkyl, each of which is substituted with 0, 1 or 2 substituents independently selected from hydroxy, halogen, Cι-C₄alkyl and haloCι-C₄alkyl; and (v) n is 1. Preferred compounds of Formulas lb, lib, Illb and IVb have an IC₅₀ value of 100 nanomolar or less, 10 nanomolar or less or 1 nanomolar or less in a capsaicin receptor calcium mobilization assay. hi another aspect, the invention provides pharmaceutical compositions, comprising at least one compound or salt according to Formulae lb, lib, Illb, or INb, in combination with a physiologically acceptable carrier or excipient.

Within each of the methods described herein, the VRl modulator(s) employed may satisfy one or more ofthe following formulas: I, II, III, TV, la, Ila, Ilia, TV a, lb, lib, Illb or IVb. Certain such modulators satisfy Formula I, in which

V, X, W,Y and Z are each independently Ν or CR_, with the proviso that at least one of V and X is Ν;

U is Ν or CR₂, with the proviso that if V and X are Ν, then U is CR₂;

Ri is independently selected at each occurrence from hydrogen, halogen, hydroxy, cyano, amino, Cι-C₈alkyl, haloCι-C₈alkyl, Cι-C₈alkoxy, haloCι-C₈alkoxy and mono- and di-(Cι- C₈alkyl)amino; R₂ is: (i) hydrogen, halogen, cyano or -COOH;

(ii) Cι-C₈alkanoyl, C₂-C₈alkanone, or Ci-C₈carbamate, each of which is unsubstituted or substituted with from 1 to 9 substituents independently selected from R ; or (iii) a group ofthe formula -R_e-M-A-R_y, wherein: R_e is Co-C₃alkyl; M is a bond, N(R_), O, S, SO₂, -N(R_)-, (C=O)_pN(R_z), N(R_z)(C=O)_p, S0₂N(R_z), or N(R_z)SO2, wherein p is 0 or 1; A is a bond or Cι-C₈alkyl, optionally substituted with from 1 to 3 substituents independently selected from R_b; and R_y and R_z are:

(a) independently hydrogen, Cι-C₈alkyl, C₂-C₈alkenyl, C2-C₈ alkynyl, C₆- Cι₀arylCι-C₈alkyl, C₂-C₈alkyl ether, Cι-C₈alkoxy, a 4- to 10-membered carbocycle or heterocycle, or joined to R_e to form a 4- to 10-membered carbocycle or heterocycle, wherein each R_y and R_z is independently unsubstituted or substituted with from 1 to 9 substituents independently selected from R_b; or

(b) joined to form a 4- to 10-membered heterocycle that is unsubstituted or substituted with from 1 to 9 substituents independently selected from R_b;

Ari and Aτ₂ are independently selected from 5- to 10-membered aromatic carbocycles and heterocycles, each of which is unsubstituted or substituted with from 1 to 3 substituents independently selected from groups ofthe formula LR_a; L is independently selected at each occurrence from a bond, -O-, -C(=O)-, -OC(=O)-, -C(=0)O-, -0-C(=O)O-, -S(O)_m-, -NR_X-, -C(=O)NHR_x-, -NHR_xC(=O)-, -NR_xS(O)_m-, - S(O)_mNR_x- and -N[S(O)_mR_x]S(O)_m-; wherein m is independently selected at each occurrence from 0, 1 and 2; and R_x is independently selected at each occurrence from hydrogen and Cι-C₈alkyl; R_a is independently selected at each occurrence from: (i) hydrogen, halogen, cyano and nitro; and

(ii) Cι-C₈alkyl, C₂-C₈alkenyl, C₂-C₈alkynyl, C₂-C₈alkyl ether, 3- to 10-membered heterocycles, mono- and di-(Cι-C₈alkyl)amino and (3- to 10-membered heterocycle)Cι-

C₆alkyl, each of which is unsubstituted or substituted with from 1 to 9 substituents independently selected from R_b; and

R_b is independently chosen at each occurrence from hydroxy, halogen, amino, aminocarbonyl, amido, cyano, nitro, Cι-C₈alkyl, Cι-C₈alkoxy, Cι-C₈alkylthio, Cι-C₈alkyl ether, hydroxyCi- C₈alkyl, haloCι-C₈alkyl, phenyl, phenyl(Cι-C₈alkyl), mono-and di-(Cι-C₆alkyl)amino,

(SO₂)Ci-C₈alkyl, 5- to 7-membered heterocycle and (5- to 7-membered heterocycle)(Cι-

Cgalkyl). TERMINOLOGY

Compounds are generally described herein using standard nomenclature. For compounds having asymmetric centers, it should be understood that (unless otherwise specified) all of the optical isomers and mixtures thereof are encompassed. In addition, compounds with carbon-carbon double bonds may occur in Z- and E- forms, with all isomeric forms of the compounds being included in the present invention unless otherwise specified. Where a compound exists in various tautomeric forms, a recited compound is not limited to any one specific tautomer, but rather is intended to encompass all tautomeric forms. Certain compounds are described herein using a general formula that includes variables (e.g., Ri, n, Ari). Unless otherwise specified, each variable within such a formula is defined independently of other variable, and any variable that occurs more than one time in a formula is defined independently at each occurrence.

The term "quinazolin-4-ylamine analogue," as used herein, encompasses all compounds that satisfy one or more of Formulas I-IN herein, as well as pharmaceutically acceptable salts and hydrates of such compounds. Such compounds include analogues in which the quinazoline core is modified in the number and/or placement of ring nitrogen atoms, as well as analogues in which varied substituents, as described in more detail below, are attached to such a core structure. In other words, compounds that are pyrido[2,3-<i]pyrimidine-4-ylamines, pyrido[3,2- < |pyrimidin-4-ylamines, isoquinolin-1-ylamines and phthalazin-1-ylamines are within the scope of quinazolin-4ylamine analogues.

As used herein, the term "alkyl" refers to a straight chain, branched chain or cyclic saturated aliphatic hydrocarbon. An alkyl group may be bonded to an atom within a molecule of interest via any chemically suitable portion. Alkyl groups include groups having from 1 to 8 carbon atoms (Cι-C₈alkyl), from 1 to 6 carbon atoms (Cι-C₆alkyl) and from 1 to 4 carbon atoms (Ci-C_.alkyl), such as methyl, ethyl, propyl, isopropyl, n-butyl, sec-butyl, tert-butyl, pentyl, 2- pentyl, isopentyl, neopentyl, hexyl, 2-hexyl, 3-hexyl, 3-methylpentyl, cyclopropyl, cyclopropylmethyl, cyclopentyl, cyclop entylmethyl, cyclohexyl, cycloheptyl and norbornyl. "Co-C₄alkyl" refers to a bond or a Cι-C₄alkyl group; "Co-C₈alkyl" refers to a bond or a Ci- C₈alkyl group. Similarly, "alkenyl" refers to straight or branched chain alkene groups or cycloalkene groups. Within an alkenyl group, one or more unsaturated carbon-carbon double bonds are present. Alkenyl groups include C₂-C₈alkenyl, C2-C6alkenyl and C2-C4alkenyl groups, which have from 2 to 8, 2 to 6 or 2 to 4 carbon atoms, respectively, such as ethenyl, allyl or isopropenyl. "Alkynyl" refers to straight or branched chain alkyne groups, which have, one or more unsaturated carbon-carbon bonds, at least one of which is a triple bond. Alkynyl groups include C₂-C₈alkynyl, C2-C6alkynyl and C2-C4alkynyl groups, which have from 2 to 8, 2 to 6 or 2 to 4 carbon atoms, respectively.

By "alkoxy," as used herein, is meant an alkyl, alkenyl or alkynyl group as described above attached via an oxygen bridge. Alkoxy groups include Cι-C₈alkoxy, Cι-C₆alkoxy and - C alkoxy groups, which have from 1 to 8, 1 to 6 or 1 to 4 carbon atoms, respectively. Alkoxy groups include, for example, methoxy, ethoxy, propoxy, isopropoxy, n-butoxy, sec-butoxy, tert- butoxy, n-pentoxy, 2-pentoxy, 3-pentoxy, isopentoxy, neopentoxy, hexoxy, 2-hexoxy, 3-hexoxy, and 3-methylpentoxy. The term "alkanoyl" refers to an acyl group in a linear, branched or cyclic arrangement

(e.g., -(C=O)-alkyl). Alkanoyl groups include C₂-C₈alkanoyl, C2-C6alkanoyl and C2- C4alkanoyl groups, which have from 2 to 8, 2 to 6 or 2 to 4 carbon atoms, respectively. "Ciaikanoyr refers to -(C=O)-H, which is encompassed by the term "Cι-C₈alkanoyl."

An "alkanone" is a ketone group in which carbon atoms are in a linear, branched or cyclic alkyl arrangement. "C -C₈alkanone," "C₃-C₆alkanone" and "C₃-C₄alkanone" refer to an alkanone having from 3 to 8, 6 or 4 carbon atoms, respectively. By way of example, a C₃ alkanone group has the structure -CH₂-(C=O)-CH₃.

Similarly, "alkyl ether" refers to a linear or branched ether substituent linked via a carbon-carbon bond. Alkyl ether groups include C₂-C₈alkyl ether, C₂-C₆alkyl ether and C - C₆alkyl ether groups, which have 2 to 8, 6 or 4 carbon atoms, respectively. By way of example, a C₂ alkyl ether group has the stracture -CH₂-O-CH₃.

The term "alkoxycarbonyl" refers to an alkoxy group linked via a carbonyl (i.e., a group having the general structure -C(=O)-O-alkyl). Alkoxycarbonyl groups include C₂-C₈, C₂-C₆ and C₂-C₄alkoxycarbonyl groups, which have from 2 to 8, 6 or 4 carbon atoms, respectively. "Cialkoxycarbonyl" refers to -C(=O)-OH, which is encompassed by the term "C_-

C₈alkoxycarbonyl. "

"Alkanoyloxy," as used herein, refers to an alkanoyl group linked via an oxygen bridge

(i.e., a group having the general structure -O-C(=O)-alkyl). Alkanoyloxy groups include Q_-

C₈, C₂- and C₂-C₄alkanoyloxy groups, which have from 2 to 8, 6 or 4 carbon atoms, respectively. "Cialkanoyloxy" refers to O-C(=O)-H, which is encompassed by the term "Ci-

C₈alkanoyloxy."

The term "Ci-C₈carbonate" refers to an alkoxycarbonyl group linked via an oxygen bridge, hi other words, a carbonate group has the general structure -O-C(=O)-O-alkyl. C_- C₆carbonate groups are generally preferred, with Ci-C₄carbonate groups particularly preferred. The term "Ci-C₈carbamate," as used herein, refers to a group having the general structure -N-C(=O)-O-alkyl. Cι-C₆carbamate groups are generally preferred, with C_- C₄carbamate groups particularly preferred.

Alkylamino refers to a secondary or tertiary amine having the general structure -NH-alkyl or -N(alkyl)(alkyl), wherein each alkyl may be the same or different. Such groups include, for example, mono- and di-(Cι-C₈alkyl)amino groups, in which each alkyl may be the same or different and may contain from 1 to 8 carbon atoms, as well as mono- and di-(Cι- C₆alkyl)amino groups and mono- and di-(Cι-C₄alkyl)amino groups. Alkylaminoalkyl refers to an alkylamino group linked via an alkyl group (i.e., a group having the general structure -alkyl- NH-alkyl or -alkyl-N(alkyl)(alkyl)). Such groups include, for example, mono- and di-(Cι- C₈alkyl)aminoCι-C₈alkyl, mono- and di-(Cι-C₆alkyl)aminoCι-C₆alkyl and mono- and di-(Cι- C₄alkyl)aminoCι-C₄alkyl, in which each alkyl may be the same or different.

The term "aminocarbonyl" refers to an amide group (i.e., -(C=O)NE_₂).

The term "halogen" includes fluorine, chlorine, bromine and iodine. A "haloalkyl" is a branched, straight-chain or cyclic alkyl group, substituted with 1 or more halogen atoms (e.g., "haloCι-C₈alkyl" groups have from 1 to 8 carbon atoms; "haloCι-C₆alkyl" groups have from 1 to 6 carbon atoms). Examples of haloalkyl groups include, but are not limited to, mono-, di- or tri-fluoromethyl; mono-, di- or tri-chloromethyl; mono-, di-, tri-, terra- or penta-fluoro ethyl; and mono-, di-, tri-, tetra- or penta-chloroethyl. Typical haloalkyl groups are trifluoromethyl and difluoromethyl. Within certain compounds provided herein, not more than 5 or 3 haloalkyl groups are present. The term "haloalkoxy" refers to a haloalkyl group as defined above attached via an oxygen bridge. "HaloCι-C₈alkoxy" groups have 1 to 8 carbon atoms.

A dash ("-") that is not between two letters or symbols is used to indicate a point of attachment for a substituent. For example, -CONH₂ is attached through the carbon atom. A "heteroatom," as used herein, is oxygen, sulfur or nitrogen.

A "carbocycle" or "carbocychc group" comprises at least one ring formed entirely by carbon-carbon bonds (referred to herein as a carbocychc ring), and does not contain a heterocyclic ring. Unless otherwise specified, each carbocyclic ring within a carbocycle may be saturated, partially saturated or aromatic. A carbocycle generally has from 1 to 3 fused, pendant or spiro rings, carbocycles within certain embodiments have one ring or two fused rings. Typically, each ring contains from 3 to 8 ring members (i.e., C₃-C₈); C₅-C₇ rings are recited in certain embodiments. Carbocycles comprising fused, pendant or spiro rings typically contain from 9 to 14 ring members. Certain representative carbocycles are cycloalkyl (i.e., groups that comprise saturated and/or partially saturated rings, such as cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl, cyclooctyl, adamantyl, decahydro-naphthalenyl, octahydro-indenyl, and partially saturated variants of any of the foregoing, such as cyclohexenyl), as well as aromatic groups (i.e., groups that contain at least one aromatic carbocychc ring, such as phenyl, benzyl, naphthyl, phenoxyl, benzoxyl, phenylethanonyl, fluorenyl, indanyl and 1,2,3,4- tetrahydro-naphthyl. Carbon atoms present within a carbocychc ring may, of course, be further bonded to zero, one or two hydrogen atoms and/or any of a variety of ring substituents, such as hydroxy, halogen, cyano, nitro, Cι-C₈alkyl, C₂-C₈alkenyl, C₂-Calkynyl, Cι-C₈alkoxy, C₂- C₈alkyl ether, C₃-C₈alkanone, Cι-C₈alkylthio, amino, mono- or di-(Cι-C₈alkyl)amino, C₃- C cycloalkylCo-C₄alkyl, haloCι-C₈alkyl, haloCι-C₈alkoxy, aminoCι-C₈alkyl, hydroxyCi- C₈alkyl, Cι-C₈alkanoyl, Cι-C₈alkoxycarbonyl, -COOH, -C(=O)NH₂, mono- or di-(Cι- C₈alkyl)carboxamido, -S(O₂)NH₂, and/or mono- or di-(Cι-C₈alkyl)sulfonamido.

Certain carbocycles recited herein include C₆-Cι₀arylCo-C₈alkyl groups (i.e., groups in which a carbocychc group comprising at least one aromatic ring is linked via a direct bond or a Cι-C₈alkyl group). Such groups include, for example, phenyl and indanyl, as well as groups in which either ofthe foregoing is linked via Cι-C₈alkyl, preferably via Cι-C₄alkyl. Phenyl groups linked via a direct bond or alkyl group may be designated phenylC₀-C₈alkyl (e.g., benzyl, 1- phenyl-ethyl, 1 -phenyl-propyl and 2-phenyl-ethyl).

A "heterocycle" or "heterocyclic group" has from 1 to 3 fused, pendant or spiro rings, at least one of which is a heterocyclic ring (i.e., one or more ring atoms is a heteroatom, with the remaining ring atoms being carbon). Typically, a heterocyclic ring comprises 1-4 heteroatoms; within certain embodiments each heterocyclic ring has 1 or 2 heteroatoms per ring. Each heterocyclic ring generally contains from 3 to 8 ring members (rings having from 5 to 7 ring members are recited in certain embodiments), and heterocycles comprising fused, pendant or spiro rings typically contain from 9 to 14 ring members. Heterocycles may be optionally substituted at nitrogen and/or carbon atoms with a variety of substituents, such as those described above for carbocycles. Unless otherwise specified, a heterocycle may be a heterocycloalkyl group (i.e., each ring is saturated or partially saturated) or a heteroaryl group (i.e., at least one ring within the group is aromatic). A heterocyclic group may generally be linked via any ring or substituent atom, provided that a stable compound results. N-linked heterocyclic groups are linked via a component nitrogen atom. A "heterocycleCo-C₈alkyl" is a heterocyclic group linked via a direct bond or Cι-C₈alkyl group. A (3- to 10-membered heterocycle)Cι-C₆aikyl is a heterocyclic group having from 3 to 10 ring members linked via a Cι-C₆alkyl group Heterocyclic groups include, for example, acridinyl, azepanyl, azocinyl, benzimidazolyl, benzimidazolinyl, benzisothiazolyl, benzisoxazolyl, benzofuranyl, benzothiofuranyl, benzothiophenyl, benzoxazolyl, benzothiazolyl, benzotriazolylcarbazolyl, benztetrazolyl, NH-carbazolyl, carbolinyl, chromanyl, chromenyl, cinnolinyl, decahydroquinolinyl, dihydrofuro[2,3-b]tetralιydro furan, dihydroisoquinolinyl, dihydrotetrahydrofuranyl, l,4-dioxa-8- aza-spiro[4.5]dec-8-yl, dithiazinyl, furanyl, furazanyl, imidazolinyl, imidazolidinyl, imidazolyl, indazolyl, indolenyl, indolinyl, indolizinyl, indolyl, isobenzofiiranyl, isochromanyl, isoindazolyl, isoindolinyl, isoindolyl, isothiazolyl, isoxazolyl, isoquinolinyl, morpholinyl, naphthyridinyl, octahydroisoquinolinyl, oxadiazolyl, oxazolidinyl, oxazolyl, phenanthridinyl, phenanthrolinyl, phenazinyl, phenothiazinyl, phenoxathiinyl, phenoxazinyl, phthalazinyl, piperazinyl, piperidinyl, piperidinyl, piperidonyl, pteridinyl, purinyl, pyranyl, pyrazinyl, pyrazolidinyl, pyrazolinyl, pyrazolyl, pyridazinyl, pyridoimidazolyl, pyridooxazolyl, pyridothiazolyl, pyridyl, pyrimidyl, pyrrolidinyl, pyrrolidonyl, pyrrolinyl, pyrrolyl, quinazolinyl, quinolinyl, quinoxahnyl, quinuclidinyl, tetrahydroisoquinolinyl, tetrahydroquinolinyl, tefrazolyl, thiadiazinyl, thiadiazolyl, thianthrenyl, thiazolyl, thienothiazolyl, thienooxazolyl, thienoimidazolyl, thienyl, thiophenyl, thiomorpholinyl and variants thereof in which the sulfur atom is oxidized, triazinyl, xanthenyl and any of the foregoing that are substituted with from 1 to 4 substituents as described above.

Certain heterocyclic groups are 3- to 10-membered or 5- to 10-membered groups that contain 1 heterocyclic ring or 2 fused or spiro rings, optionally substituted as described above. (C₃-Cιo)heterocycloalkyls include, for example, piperidinyl, piperazinyl, pyrrolidinyl, azepanyl, l,4-dioxa-8-aza-spiro[4.5]dec-8-yl, morpholino, thiomorpholino, and 1,1-dioxo-thiomorpholin- 4-yl, as well as groups in which each ofthe foregoing is substituted with from 1 to 6 (preferably from 1 to 4) substituents independently selected from halogen, hydroxy, Cι-C₄alkyl, Ci- C₄alkoxy, haloCι-C₄alkyl, haloCι-C₄alkoxy, Cι-C₄alkylcarbonyl and Cι-C₄alkoxycarbonyl. In certain embodiments, a heterocycloalkyl may be a 4- to 7-membered heterocycloalkylCo-C₄alkyl group. Such groups comprise a 4- to 7-membered heterocycloalkyl group as described above, linked via a direct bond or a C₁-C₄ alkyl group.

Certain aromatic heterocycles include 5- to 10-membered heteroarylCo-C₈alkyl groups (i.e., groups in which the heterocyclic group comprising at least one aromatic ring is linked via a direct bond or a Cι-C₈alkyl group). Such groups include, for example, the heteroaryl groups recited above, as well as groups in which any of the foregoing is linked via Cι-C₈alkyl, C_- C₆alkyl or Cι-C₄alkyl. Representative aromatic heterocycles are azocinyl, pyridyl, pyrimidyl, imidazolyl, tefrazolyl and 3,4-dihydro-lH-isoquinolin-2-yl, as well as groups in which each of the foregoing is linked via Cι-C₄alkyl.

A "substituent," as used herein, refers to a molecular moiety that is covalently bonded to an atom within a molecule of interest. For example, a "ring substituent" may be a moiety such as a halogen, alkyl group, haloalkyl group or other group discussed herein that is covalently bonded to an atom (preferably a carbon or nitrogen atom) that is a ring member. The term "substitution" refers to replacing a hydrogen atom in a molecular structure with a substituent as described above, such that the valence on the designated atom is not exceeded, and such that a chemically stable compound (i.e., a compound that can be isolated, characterized, and tested for biological activity) results from the substitution.

Groups that are "optionally substituted" are unsubstituted or are substituted by other than hydrogen at one or more available positions, typically 1, 2, 3, 4 or 5 positions, by one or more suitable groups (which may be the same or different). Such optional substituents include, for example, hydroxy, halogen, cyano, nitro, Cι-C₈alkyl, C₂-C₈alkenyl, C₂-C₈alkynyl, Cι-C₈alkoxy, C₂-C₈alkyl ether, C₃-C₈alkanone, Cι-C₈alkylthio, amino, mono- or di-(Cι-C₈alkyl)amino, haloCι-C₈alkyl, haloCι-C₈alkoxy, Cι-C₈alkanoyl, Cι-C₈alkanoyloxy, Cι-C₈alkoxycarbonyl, - COOH, -CONH₂, mono- or di-(Cι-C₈alkyl)carboxamido, -SO₂NH₂, and/or mono or di(Cι- C₈alkyl)sulfonamido, as well as carbocychc and heterocyclic groups. Certain optionally substituted groups are substituted with from 0 to 3 independently selected substituents. The tenns "VRl," "type 1 vanilloid receptor" and "capsaicin receptor" are used interchangeably herein. Unless otherwise specified, these terms encompass both rat and human VRl receptors (e.g., GenBank Accession Numbers AF327067, AJ277028 and NM_018727; sequences of certain human VRl cDNAs are provided in SEQ ID NOs:l-3, and the encoded amino acid sequences shown in SEQ ID NOs:4 and 5, of U.S. Patent No. 6,482,611), as well as homologs thereof found in other species.

A "VRl modulator," also refened to herein as a "modulator," is a compound that modulates VRl activation and/or VRl -mediated signal transduction. A VRl modulator may be a VRl agonist or antagonist although, for certain purposes described herein, a VRl modulator preferably inhibits VRl activation resulting from binding of a vanilloid ligand agonist (e.g., capsaicm or a capsaicin analogue such as olvanil or resiniferatoxin) to VRl. A modulator binds with "high affinity" if the Kj at VRl is less than 1 micromolar, preferably less than 100 nanomolar, 10 nanomolar or 1 nanomolar. A representative assay for determining Ki at VRl is provided in Example 5, herein. A modulator is considered an antagonist if it detectably inhibits vanilloid ligand binding to VRl and/or VRl -mediated signal transduction (using, for example, the representative assay provided in Example 6); in general, such an antagonist inhibits VRl activation with a IC₅₀ value of less than 1 micromolar, preferably less than 100 nanomolar, and more preferably less than 10 nanomolar or 1 nanomolar within the assay provided in Example 6. VRl antagonists include neutral antagonists and inverse agonists. Prefened modulators do not substantially inhibit activity of human epidermal growth factor (EGF) receptor tyrosine kinase (i.e., IC₅₀ in an EGF receptor assay is greater than 1 micromolar, preferably greater than 100 micromolar or 10 micromolar). More preferably, a modulator does not detectably inhibit EGF receptor activity. Assays for detecting an inhibitory effect on EGF receptor are well known in the art, and include those described by Carpenter et al. (1979) J. Biol. Chem. 254:4884, as well as U.S. Patent Nos. 5,654,307 and 6,169,091.

An "inverse agonist" of VRl is a compound that inhibits the activity of vanilloid ligand at VRl, and reduces the activity of VRl below its basal activity level in the absence of added vanilloid ligand. Inverse agonists of VRl may also inhibit binding of vanilloid ligand to VRl . The ability of a compound to inhibit the binding of vanilloid ligand to VRl may be measured by a binding assay, such as the binding assay given in Example 5. The basal activity of VRl, as well as the reduction in VRl activity due to the presence of VRl antagonist, may be determined from a calcium mobilization assay, such as the assay of Example 6.

A "neutral antagonist" of VRl is a compound that inhibits the activity of vanilloid ligand at VRl, but does not significantly change the basal activity ofthe receptor (i.e., within a calcium mobilization assay as described in Example 6 performed in the absence of vanilloid ligand, VRl activity is reduced by no more than 10%, 5% or 2%; preferably there is no detectable reduction in activity. Neutral antagonists of VRl may inhibit the binding of vanilloid ligand to VRl.

As used herein an "agonist" of VRl is a compound that elevates the activity of the receptor above the basal activity level ofthe receptor. A "vanilloid ligand" is capsaicin or any capsaicin analogue that comprises a phenyl ring with two oxygen atoms bound to adjacent ring carbons, and that binds to VRl with a Kj (determined as described herein) that is no greater than 10 μM. Vanilloid ligand agonists include capsaicin, olvanil, N-arachidonoyl-dopamine and resiniferatoxin (RTX). Vanilloid ligand antagonists include capsazepine and iodo-resiniferatoxin. A "prodrug" is a compound that may not fully satisfy the structural requirements of the compounds provided herein, but is modified in vivo, following administration to a patient, to produce a quinazolin-4-ylamine analogue. For example, a prodrug may be an acylated derivative of a compound as provided herein. Prodrugs include compounds wherein hydroxy, amine or sulfhydryl groups are bonded to any group that, when administered to a mammalian subject, cleaves to form a free hydroxyl, amino, or sulfhydryl group, respectively. Examples of prodrugs include, but are not limited to, acetate, formate and benzoate derivatives of alcohol and amine functional groups within the compounds provided herein.

A "patient" is any individual treated with a VRl modulator as provided herein. Patients include humans, as well as other animals such as companion animals (e.g., dogs and cats) and livestock. Patients may be experiencing one or more symptoms of a condition responsive to capsaicin receptor modulation (e.g., pain, exposure to vanilloid ligand, itch, urinary incontinence, respiratory disorders, cough and or hiccup), or may be free of such symptom(s) (i.e., treatment may be prophylactic). VRl MODULATORS

As noted above, the present invention provides VRl modulators (i.e., compounds that modulate VRl -mediated signal transduction; preferably compounds that also detectably bind to VRl). VRl modulators may be used to modulate VRl activity in a variety of contexts, including in the treatment of (i) pain (e.g., neuropathic or peripheral nerve-mediated pain), (ii) exposure to capsaicin, (iii) exposure to acid, heat, light, tear gas air pollutants, pepper spray or related agents, (iv) respiratory conditions such as asthma or chronic obstructive pulmonary disease, (v) itch, (vi) urinary incontinence, (vii) cough or hiccup and/or (viii) obesity. VRl modulators may also be used within a variety of in vitro assays (e.g., assays for receptor activity), as probes for detection and localization of VRl and as standards in assays of ligand binding and VRl -mediated signal transduction.

VRl modulators provided herein are substituted quinazolin-4-ylamine analogues that detectably modulate the binding of capsaicin to VRl at nanomolar (i.e., submicromolar) concentrations, preferably at subnanomolar concentrations, more preferably at concentrations below 100 picomolar, or even below 20 picomolar. Such modulators are preferably not capsaicin analogs (i.e., they do not comprise a phenyl ring with two oxygen atoms bound to adjacent ring carbons). Prefened modulators are VRl antagonists and have no detectable agonist activity in the assay described in Example 6. In certain embodiments, such modulators further bind with high affinity to VRl, and do not substantially inhibit activity of human EGF receptor tyrosine kinase. The present invention is based, in part, on the discovery that small molecules having the general Formula I (as well as pharmaceutically acceptable salts, hydrates and prodrugs thereof) modulate VRl activity. In certain embodiments, no more than 2 of W, Y and Z are N. Representative quinazoline-4-ylamine analogues include, but are not limited to, compounds in which U, V, W, X, Y and Z are as indicated for any one ofthe embodiments listed in Table I. Table I

Representative Quinazoline-4-ylamine Analogue Core Stractures

Within Formulas I-1N, Ri is preferably hydrogen, Cι-C₄alkyl or haloCι-C₄alkyl, with hydrogen particularly prefened. For example, N and X may both be Ν. Within certain such embodiments, one of W, Y or Z is Ν and the others are CH, or all three of W, Y and Z are CH.

In certain embodiments, R₂ in Fonnulas I and II, if present, is hydrogen, amino, hydroxy, halogen, or optionally substituted -(CH₂)_nΝH₂, -(CH₂)_nNH(Cι-C₈alkyl),

-(CH₂)_nN(Cι-C₈alkyl)₂, -(CH₂)_n(5- to 8-membered heterocycloalkyl), -(CH₂)_nOH, -(CH₂)_nO(Cι-C₈alkyl). Optionally substituted groups include, for example, unsubstituted groups and groups substituted with from 1 to 4 substituents independently chosen from halogen, cyano, hydroxy, amino, mono- and di-(Cι-C₆alkyl)amino, Cι-C₆alkyl, and haloCι-C₆alkyl. Heterocycloalkyl groups include those in which the heterocycloalkyl comprises a nitrogen or oxygen atom directly linked to the -(CH₂)_. Within certain embodiments, Ari and Ar₂ in compounds of Formulas I-IN are independently selected from 5- to 7-membered aromatic carbocycles and heterocycles, optionally substituted. For example, Ari and Ar₂ may be independently selected from phenyl and 6-membered aromatic heterocycles, each of which is substituted with 0, 1 or 2 substituents. hi certain embodiments, Ar_. is phenyl or pyridyl, each of which is unsubstituted or substituted with 1, 2 or 3 substituents as described above; preferably such substituents, if any, are independently selected from halogen, hydroxy, cyano, amino, nitro, mono- and di-(Cι- C₆alkyl)amino, Cι-C₆alkyl, haloCι-C₆alkyl, Cι-C₆alkoxy and haloCi-C_όalkoxy. For example, Ari may contain one substituent selected firom halogen, Cι-C₆alkyl, Cι-C₆alkoxy, haloCi- C_δalkyl and haloCi-Cealkoxy. If one or more A^ substituents is present, at least one such substituent is preferably located in the ortho position (e.g., Ar_. may be phenyl substituted at the 2-position, or 2-pyridyl substituted at the 3-position). Ari groups include, but are not limited to, pyridin-2-yl, 3-methyl-pyridin-2-yl, 3-trifluoromethyl-pyridin-2-yl and 3-halo-pyridin-2-yl.

AJ"₂, within certain embodiments, is phenyl or pyridyl, each of which is unsubstituted or substituted with 1 or 2 substituents as described above. In certain embodiments, one such substituent is located in the para position of a 6-membered Ar₂; in Formula II the -SO₂-R₇ is preferably in the para position, and in Formulas I, III and IN one of the optional substituents is preferably located in that position. Optional Ar₂ substituents are as described above and include, for example, groups in which R_a is independently selected at each occurrence from: (i) hydrogen, halogen, cyano and nitro; and (ii) Cι-C₈alkyl, C₂-C₈alkenyl, C₂-C₈alkynyl and 3- to 10-membered heterocycles, each of which is optionally substituted with from 1 to 9 substituents independently selected from hydroxy, halogen, Cι-C₆alkyl and haloCι-C₆alkyl. Prefened R_a moieties include halogen, hydroxy, cyano, amino, mono- and di-(Cι-C₆alkyl)amino, Cι-C₆alkyl, haloCi-C_.alkyl, Cι-C₆alkoxy, haloCι-C₆alkoxy, C₂-C₆alkyl ether, Cι-C₆alkanoyl, -(SO₂)R_a, -ΝR_xS(O)_m, and -N(S(O)_m)₂; wherein m is 1 or 2, R_x is hydrogen or Cι-C₆alkyl, and R_a is Cι-C₆alkyl, haloCι-C₆alkyl, or a 5- to 10-membered, N-linked heterocyclic group, each of which R_a is optionally substituted with from 1 to 4 substituents independently chosen from R . Prefened Aτ₂ substituents include Cι-C₄alkyl, haloCι-C₄alkyl and groups of the formula - (SO₂)R_a, wherein R_a is Cι-C₄alkyl or haloCι-C₄alkyl. Ar₂ groups include phenyl, pyridyl, pyridazinyl, pyrimidinyl, pyrazinyl, pynolyl, imidazolyl, pyrazolyl, oxazolyl, isoxazolyl, thiazolyl, isothiazolyl and thiadiazolyl, each of which is optionally substituted with 1 or 2 substituents independently selected from halogen, cyano, Cι-C₆alkyl, haloCι-C₆alkyl, C_- C₆alkoxy, haloCι-C₆alkoxy, -SO₂-R_ and -SO₂NR_x-R_a. Prefened Ar₂ groups are phenyl, pyridyl, isoxazolyl, thiadiazolyl and pyrazolyl, optionally substituted with halogen, Cι-C₄alkyl or haloCι-C₄alkyl. Within certain embodiments, Ar₂ is phenyl or pyridyl, each of which is optionally substituted with 1 or 2 substituents independently chosen from halogen, cyano, Q- C₄alkyl, haloCι-C₄alkyl, C₂-C₄alkyl ether, Cι-C₄alkanoyl and groups of the formula -(SO₂)R_a, wherein R_a is Cι-C₆alkyl or haloCι-C₆alkyl. Ar₂ groups include, but are not limited to, phenyl, 2-pyridyl and 3 -pyridyl, each of which is substituted at the 4-position with halogen, cyano, methyl, ethyl, propyl, isopropyl, t-butyl, trifluoromethyl, 2,2,2-trifluoroethyl, 2,2,2-trifluoro-l- methyl-ethyl, methanesulfonyl, ethanesulfonyl, propanesulfonyl, propane-2-sulfonyl, trifluoromethanesulfonyl or 2,2,2-trifluoroethanesulfonyl.

Within certain embodiments of compounds of Formula II, Ar₂ is phenyl or pyridyl, optionally substituted with 1 or 2 substituents independently chosen from halogen, cyano, C_- C₆alkyl, and haloCι-C₆alkyl; and Ari is phenyl or pyridyl, optionally substituted with halogen, Cι-C₆alkyl, haloCι-C₆alkyl, Cι-C₆alkoxy or haloCι-C₆alkoxy. Within Formula II, the carbon atom of Ar₂ that is linked to the SO₂ is preferably separated from the carbon atom linked to the N by at least one, and preferably two, ring atoms, hi other words, for embodiments in which Ar₂ is phenyl, the SO₂ is preferably located at the para (4) position. In certain embodiments, R₇ is Cι-C₆alkyl, haloCi-C_.alkyl, amino, mono or di(Cι-C₆alkyl)amino or a nonaromatic heterocycle (e.g., morpholinyl, piperidinyl, piperazinyl or pyrrolidinyl), optionally substituted as described above.

Within certain embodiments, R₃ and R₄ of Formula III are each independently selected from (i) hydrogen or (ii) Cι-C₈alkyl, C₂-C₈alkenyl, C₂-C₈alkynyl, C_-C₈alkanone, Ci- C₈alkanoyl, C₂-C₈alkyl ether, C₆-Cι₀arylC₀-C₈alkyl, 5- to 10-membered heterocycleC₀-C₈alkyl and -(SO₂)Cι-C₈alkyl, each of which is optionally substituted with from 1 to 9 substituents independently selected from R_b. Within other embodiments, R and R₄ are each independently selected from (i) hydrogen and (ii) Cι-C₈alkyl, C₂-C₈alkenyl, phenylC₀-C₄alkyl, indanylCo- C₄alkyl, 5- to 6-membered heteroarylC₀-C₄alkyl and 4- to 7-membered heterocycloalkylC₀- C₄alkyl, each of which is optionally substituted with from 1 to 4 substituents independently selected from hydroxy, halogen, amino, Cι-C₆alkyl, haloCι-C₆alkyl, Cι-C₆alkoxy and haloCi- C₆alkoxy. Representative R₃ and R₄ groups include Cι-C₆alkyl, C₂-C₆alkenyl, 5- to 7- membered heterocycloC₀-C₄alkyl, C₂-C₆alkyl ether, indanyl, benzyl, 1-phenyl-ethyl, 1 -phenyl- propyl and 2-phenyl-ethyl, each of which is unsubstituted or substituted with from 1 to 3 substituents independently selected from hydroxy, halogen and Cι-C₄alkyl. For example, at least one of R₃ and _t may be pyridylCo-C alkyl, pyrimidylCo-C₄alkyl, imidazolylC₀-C alkyl or tetrazolylC₀-C₄alkyl, each of which is substituted with 0, 1 or 2 substituents. Alternatively, R₃ and/or may be joined to an R₅ or Rζ group (along with the N to which R and R₄ are bound and any carbon atoms between the N and R₅ or Re) to form an optionally substituted heterocycle, such as a 5- to 10-membered mono- or bi-cyclic group. Within other embodiments, R₃ and or R_. of Formula III may form an optionally substituted heterocycle. For example, R₃ and R₄ may be joined to form, with the N to which they are bound, an optionally substituted heterocycle; or R₃ or _t may be joined to an R₅ or R₆ moiety to from an optionally substituted heterocycle. hi either case, the resulting heterocycle may be, for example, a 4- or 5- to 10-membered, mono- or bi-cyclic group substituted with firom 0 to 4 substituents (e.g., from 1 to 4 substituents or 0, 1 or 2 substituents). hi certain embodiments, each substituent is independently selected from hydroxy, halogen, Cι-C₄alkyl, halo -Gjalkyl, Cι-C alkoxy, haloCι-C₄alkoxy, Cι-C₄alkanoyl, Cι-C₄alkoxycarbonyl, aminocarbonyl, heterocycleC₀-C₈alkyl and heterocycleCι-C₈alkoxycarbonyl. In certain embodiments, such substituents are lower alkyl groups such as methyl and or ethyl.

A heterocyclic group that comprises R and/or R₄ may be a heteroaryl group, which comprises an aromatic ring (e.g., optionally substituted acridinyl, benzimidazolinyl, benzimidazolyl, benzotriazolyl, carbazolyl, cinnolinyl, indazolyl, indolinyl, indolyl, isoquinolinyl, quinoxahnyl, naphthyridinyl, phenanthridinyl, phenazinyl, phenothiazinyl, phenoxazinyl, phthalazinyl, pteridinyl, purinyl, qumolinyl, quinoxahnyl, quinazolinyl, tetrahydroisoquinolinyl or tetrahydroquinolinyl). One such heteroaryl is 3,4-dihydro-lH- isoquinolin-2-yl. Alternatively, the heterocycle may be an optionally substituted heterocycloalkyl group, such as azepanyl, azocinyl, decahydroquinolinyl, l,4-dioxa-8-aza- spiro[4.5]dec-8-yl, imidazolidinyl, imidazolinyl, morpholino, piperadinyl, piperazinyl, pyridazinyl, pyrazohdinyl, pyrazolinyl, pynolidinyl, pynolinyl, thiomorpholino or 1,1-dioxo- thiomorpholin-4-yl. Representative heterocycles that may be formed from R₃ and R₄ include, but are not limited to, optionally substituted azepane, azocane, dihydroisoquinoline, imidazole, morpholine, octahydroquinoline, piperazine, piperidine and pynolidine. Representative heterocycles that may be formed from R or R₄, in combination with an R₅ or Re, include (but are not limited to) optionally substituted piperidine and pyrrolidine.

R₅ and R₆ of Formula III, within certain embodiments, are independently (at each occurrence) hydrogen or optionally substituted Cι-C₆alkyl; in addition, or alternatively, any R₅ or R₆ may be joined with any other R5 or R₆ to form an optionally substituted 5- to 7-membered cycloalkyl, or (as discussed above) joined with R or ₄ to form an optionally substituted heterocycle.

Within Formula IN, R₃ may be (in certain embodiments) (i) hydrogen or (ii) Cι-C₈alkyl, C₂-C_alkenyl, C₂-Csahcynyl, C₃-C₈alkanone, C₂-C₈alkyl ether, C_δ-CioarylCo-Csalkyl, or 5- to 10- membered heterocycleC₀-C₈alkyl, each of which is optionally substituted with from 1 to 9 substituents independently selected firom R . Within other embodiments, R₃ of Formula IN is (i) hydrogen or (ii) C C_.alkyl, C₂-C₆alkyl ether, phenylCo-C alkyl, 5- to 6-membered heteroarylC_-C alkyl, or 4- to 7-membered heterocycloalkylCo-C₄alkyl, each of which is optionally substituted with from 1 to 4 substituents independently selected firom hydroxy, halogen, amino, Cι-C₆alkyl, haloCι-C₆alkyl, Ci-C_δalkoxy and haloC_-C₆alkoxy. Representative R₃ groups include hydrogen, Cι-C₄alkyl, Cι-C alkyl ether and benzyl, each of which is unsubstituted or substituted with from 1 to 3 substituents independently selected firom hydroxy, halogen and Cι-C₄alkyl. Alternatively, R₃ may be joined to an R₅ or R₆ group (along with the O to which R₃ is bound and any carbon atoms between the O and R₅ or R₆) to form an optionally substituted heterocycle, such as a 5- to 10-membered mono- or bi-cyclic group. The resulting heterocycle may, for example, be substituted with from 0 to 4 (e.g., 0, 1 or 2) substituents independently chosen from hydroxy, halogen, Cι-C₄alkyl, haloCι-C₄alkyl, Cι-C₄alkoxy, haloCi- C alkoxy, Cι-C₄alkanoyl, Cι-C₄alkoxycarbonyl, aminocarbonyl, heterocycleC₀-C₈alkyl and heterocycleCi-Csalkoxycarbonyl.

R₅ and R_δ, within certain embodiments of Formula IV, are independently (at each occunence) hydrogen or optionally substituted Cι-C₆alkyl; in addition, or alternatively, any R₅ or R₆ may be joined with any other R₅ or R₆ to form an optionally substituted 5- to 7-membered cycloalkyl, or (as discussed above) joined with R to form an optionally substituted heterocycle. In certain such embodiments, each R₅ and R₆ is hydrogen, n may be 1, 2 or 3, with 1 prefened in certain embodiments. Certain prefened compounds satisfy at least one of Formulas la, Ila, Ilia or IN a, in which the variables are as defined above for Formulas I, II, III and IN, respectively:

Formula la Formula Ila

Formula Ilia Formula IVa

Representative compounds of Formulas I-1N include, but are not limited to, those specifically described in Examples 1-3. It will be apparent that the specific compounds recited therein are representative only, and are not intended to limit the scope of the present invention. Further, as noted above, all compounds of the present invention may be present as a hydrate, free base or a pharmaceutically acceptable acid addition salt.

Substituted quinazolin-4-ylamine analogues provided herein detectably alter (modulate) vanilloid ligand-induced VRl activity, as determined using a standard in vitro VRl ligand binding assay and/or a functional assay such as a calcium mobilization assay, dorsal root ganglion assay or in vivo pain relief assay. References herein to a "VRl ligand binding assay" are intended to refer to a standard in vitro receptor binding assay such as that provided in Example 5, and a "calcium mobilization assay" (also refened to herein as a "signal transduction assay" may be performed using the method of Example 6. Briefly, to assess VRl binding, a competition assay may be performed in which a VRl preparation is incubated with labeled (e.g., ¹²⁵I) VRl agonist and unlabeled test compound. Within the assays provided herein, the VRl used is preferably a mammalian VRl, more preferably a human or rat VRl. The receptor may be recombinantly expressed or naturally expressed. The VRl preparation may be, for example, a membrane preparation from HEK293 or CHO cells that recombinantly express human VRl (such as a VRl sequence provided in U.S. Patent No. 6,482,611).

Incubation with a compound that detectably modulates vanilloid ligand binding to VRl will result in a decrease or increase in the amount of label bound to the VRl preparation, relative to the amount of label bound in the absence of the compound. Preferably, such a compound will exhibit a K at VRl of less than 1 micromolar, more preferably less than 500 nM, 100 nM, 20 nM, 10 nM or 1 nM within a VRl ligand binding assay performed as described in Example 5. In general, compounds that decrease the amount of label bound to the VRl preparation within such an assay are prefened.

As noted above, compounds that are VRl antagonists are prefened within certain embodiments. Such compounds exhibit IC₅₀ values of 1 micromolar or less, preferably about 100 nanomolar or less, 10 nanomolar or less or 1 nanomolar or less within a standard in vitro VRl -mediated calcium mobilization assay, as provided in Example 6. Briefly, cells expressing capsaicin receptor are contacted with a compound of interest and with an indicator of intracellular calcium concentration (e.g., a membrane permeable calcium sensitivity dye such as Fluo-3 or Fura-2 (both of which are available, for example, from Molecular Probes, Eugene, OR), each of which produce a fluorescent signal when bound to Ca^""^"). Such contact is preferably carried out by one or more incubations of the cells in buffer or culture medium comprising either or both of the compound and the indicator in solution. Contact is maintained for an amount of time sufficient to allow the dye to enter the cells (e.g., 1-2 hours). Cells are washed or filtered to remove excess dye and are then contacted with a vanilloid receptor agonist (e.g., capsaicin, RTX or olvanil), typically at a concentration equal to the IC50 concentration, and a fluorescence response is measured. When cells are contacted with a compound that is a VRl antagonist, and with a vanilloid receptor agonist, the fluorescence response is generally reduced by at least 20%, preferably at least 50%) and more preferably at least 80%, as compared to cells that are contacted with the agonist in the absence of test compound. Alternatively, or in addition, compounds may be evaluated for activity using a cultured dorsal root ganglion assay as provided in Example 9 and/or an in vivo pain relief assay as provided in Example 10. Compounds provided herein preferably have a statistically significant specific effect on VRl activity within one or more such functional assays. Within certain embodiments, modulators provided herein do not substantially modulate ligand binding to other cell surface receptors, such as EGF receptor tyrosine kinase or the nicotinic acetylcholine receptor. In other words, such modulators do not substantially inhibit activity of a cell surface receptor such as the human epidermal growth factor (EGF) receptor tyrosine kinase or the nicotinic acetylcholine receptor (e.g., the IC₅₀ or IC₄₀ at such a receptor is preferably greater than 1 micromolar, and most preferably greater than 10 micromolar). Preferably, a modulator does not detectably inhibit EGF receptor activity or nicotinic acetylcholine receptor activity at a concentration of 0.5 micromolar, 1 micromolar or more preferably 10 micromolar. Assays for determining EGF receptor inhibition are well known in the art, and include those described by Carpenter et al. (1979) J. Biol. Chem. 254:4884, as well as U.S. Patent Nos. 5,654,307 and 6,169,091, and WO 95/19774. Assays for determining nicotinic acetylcholine receptor inhibition (e.g., as IC₄₀) are also well known in the art, and include those described by Liu and Simon (1997) Neuroscience Letters 228:29.

Prefened compounds of the present invention are non-sedating. In other words, a dose of such compounds that is twice the minimum dose sufficient to provide analgesia in an animal model for determining pain relief (such as a model provided in Example 10, herein) causes only transient (i.e., lasting for no more than V_ the time that pain relief lasts) or preferably no statistically significant sedation in an animal model assay of sedation (using the method described by Fitzgerald et al. (1988) Toxicology 49(2-3):433-9). Preferably, a dose that is five times the minimum dose sufficient to provide analgesia does not produce statistically significant sedation. More preferably, a compound provided herein does not produce sedation at intravenous doses of less than 25 mg/kg (preferably less than 10 mg/kg) or at oral doses of less than 140 mg/kg (preferably less than 50 mg/kg, more preferably less than 30 mg/kg).

If desired, compounds provided herein may be evaluated for certain pharmacological properties including, but not limited to, oral bioavailability (prefened compounds are orally bioavailable to an extent allowing for therapeutically effective concentrations of the compound to be achieved at oral doses of less than 140 mg/kg, preferably less than 50 mg/kg, more preferably less than 30 mg/kg, even more preferably less than 10 mg/kg, still more preferably less than 1 mg/kg and most preferably less than 0.1 mg/kg), toxicity (a prefened compound is nontoxic when a capsaicin receptor modulatory amount is administered to a subject), side effects (a prefened compound produces side effects comparable to placebo when a therapeutically effective amount of the compound is administered to a subject), serum protein binding and in vitro and in vivo half-life (a prefened compound exhibits an in vitro half-life that is equal to an in vivo half-life allowing for Q.I.D dosing, preferably T.I.D. dosing, more preferably B.I.D. dosing, and most preferably once-a-day dosing). In addition, differential penetration of the blood brain barrier may be desirable for compounds used to treat pain by modulating CNS VRl activity such that total daily oral doses as described above provide such modulation to a therapeutically effective extent, while low brain levels of compounds used to treat peripheral nerve mediated pain may be prefened (i.e., such doses do not provide brain (e.g., CSF) levels of the compound sufficient to significantly modulate VRl activity). Routine assays that are well known in the art may be used to assess these properties, and identify superior compounds for a particular use. For example, assays used to predict bioavailability include transport across human intestinal cell monolayers, including Caco-2 cell monolayers. Penetration of the blood brain barrier of a compound in humans may be predicted from the brain levels ofthe compound in laboratory animals given the compound (e.g., intravenously). Seram protein binding may be predicted from albumin binding assays. Compound half-life is inversely proportional to the frequency of dosage of a compound. In vitro half-lives of compounds may be predicted from assays of microsomal half-life as described within Example 7, herein.

Toxicity and side effects may be assessed using any standard method. In general, the term "nontoxic" as used herein shall be understood in a relative sense and is intended to refer to any substance that has been approved by the United States Food and Drug Administration ("FDA") for administration to mammals (preferably humans) or, in keeping with established criteria, is susceptible to approval by the FDA for administration to mammals (preferably humans). Toxicity may be also evaluated using the assay detecting an effect on cellular ATP production provided in Example 8. Other assays that may be used include bacterial reverse mutation assays, such as an Ames test, as well as standard teratogenicity and tumorogenicity assays. Preferably, administration of compounds provided herein at certain doses (i.e., doses yielding therapeutically effective in vivo concentrations or preferably doses of 0.01, 0.05. 0.1, 0.5, 1, 5, 10, 40, or 50 mg/kg administered parenterally or orally) does not result in prolongation of heart QT intervals (i.e., as determined by electrocardiography in guinea pigs, minipigs or dogs). When administered daily for five or preferably ten days, such doses also do not cause liver enlargement resulting in an increase of liver to body weight ratio of more than 100%>, preferably not more than 75% and more preferably not more than 50% over matched controls in laboratory rodents (e.g., mice or rats). Such doses also preferably do not cause liver enlargement resulting in an increase of liver to body weight ratio of more than 50%, preferably not more than 25%, and more preferably not more than 10% over matched untreated controls in dogs or other non-rodent mammals.

Prefened compounds also do not promote substantial release of liver enzymes (e.g., ALT, LDH, or AST) from hepatocytes in vivo. Preferably the above doses do not elevate serum levels of such enzymes by more than 100%, preferably not by more than 75% and more preferably not by more than 50% over matched untreated controls in vivo in laboratory rodents. Similarly, concentrations (in culture media or other such solutions that are contacted and incubated with cells in vitro) equivalent to two-fold, preferably five-fold, and most preferably ten- fold the minimum in vivo therapeutic concentration do not cause detectable release of any of such liver enzymes from hepatocytes in vitro into culture medium above baseline levels seen in media from untreated cells.

Prefened compounds further do not exhibit significant activity as sodium ion chamiel blockers, exhibiting less than 15 percent inhibition, and more preferably less than 10 percent inhibition, of sodium channel specific ligand (e.g., batrachotoxin, tetrodotoxin or saxitoxin) binding when present at a concentration of 4 μM or less. Assays for sodium channel specific ligand binding are well lαiown in the art. In addition, prefened compounds do not exhibit significant androgen antagonist activity (e.g., in vivo, in a Hershberger assay, or in vitro, in an assay such as that described by Nellemann et al. (2001) Toxicology 163(l):29-38). Prefened compounds exhibit less than a 15% inhibition, more preferably less than a 10% inhibition, and most preferably less than 5% inhibition of androgen receptor activation in the in vitro assay when present at concentrations of 4 μM or less. By significant activity is meant results varying from control at the p<0.1 level or more preferably at the p<0.05 level of significance as measured using a standard parametric assay of statistical significance such as a student's T test. For detection purposes, as discussed in more detail below, compounds provided herein may be isotopically-labeled or radiolabeled. Accordingly, compounds recited in Formula I may have one or more atoms replaced by an atom of the same element having an atomic mass or mass number different firom the atomic mass or mass number usually found in nature. Examples of isotopes that can be present in the compounds provided herein include isotopes of hydrogen, carbon, nitrogen, oxygen, phosphorous, fluorine and chlorine, such as H, ^JH, ^UC, ^1JC, ¹⁴C, ¹³N, ¹⁸O, ¹⁷O, ³¹P, ³²P, ³⁵S, ¹⁸F and ³⁶C1. In addition, substitution with heavy isotopes such as deuterium (i.e., ²H) can afford certain therapeutic advantages resulting firom greater metabolic stability, for example increased in vivo half-life or reduced dosage requirements and, hence, may be prefened in some circumstances.

PREPARATION OF VRl MODULATORS

Substituted quinazolin-4-ylamine analogues may generally be prepared using standard synthetic methods. In general, starting materials are commercially available from suppliers such as Sigma- Aldrich Corp. (St. Louis, MO), or may be synthesized from commercially available precursors using established protocols. By way of example, a synthetic route similar to that shown in any of Schemes 1-23 may be used, together with synthetic methods known in the art of synthetic organic chemistry, or variations thereon as appreciated by those skilled in the art. "R," in the following schemes, refers to any group consistent with the description of the compounds provided herein. In the Schemes that follow, the term "catalyst" refers to a suitable transition metal catalyst such as, but not limited to, tetrakis(triphenylphosphine)palladium(0) or palladium(II) acetate. In addition, the catalytic systems may include ligands such as, but not limited to, 2- (Dicyclohexylphosphino)biphenyl and tri-tert-butylphosphine, and may also include a base such as K₃PO₄, Na₂CO₃ or sodium or potassium tert-butoxide. Transition metal-catalyzed reactions can be carried out at ambient or elevated temperatures using various inert solvents including, but not limited to, toluene, dioxane, DMF, N-methylpynolidinone, ethyleneglycol dimethyl ether, diglyme and acetonitrile. When used in conjunction with suitable metallo-aryl reagents, transition metal-catalyzed (hetero)aryl-aryl coupling reactions can be used to prepare the compounds encompassed in general structures 1C, 2A and 2F, 3C, 5A and 5H, 14C, 15A, 16D, 18D, 19C, 20C and 22C. Commonly employed reagent/catalyst pairs include aryl boronic acid palladium(0) (Suzuki reaction; Miyaura and Suzuki (1995) Chemical Reviews 95:2457) and aryl triaUcylstannane/palladium(0) (Stille reaction; T. N. Mitchell, Synthesis (1992) 803), arylzinc/palladium(0) and aryl Grignard/nickel(II).

The term "reduce" refers to the process of reducing a nitro functionality to an amino functionality. This transformation can be carried out in a number of ways well known to those skilled in the art of organic synthesis including, but not limited to, catalytic hydrogenation, reduction with SnCl₂ and reduction with titanium trichloride. For an overview of reduction methods see: Hudlicky, M. (1996) Reductions in Organic Chemistry, ACS Monograph 188. The term "activate" refers to a synthetic transformation in which a carbonyl of an amide moiety is converted to a suitable leaving group (L). Such a transformation can be used, for example, to prepare compounds of general structure IF, 2E, 2G, 5F, 11A, 141, 15G, 16L, 17H, 191, 201, 21C and 23H. Reagents suitable for carrying out this transformation are well known to those skilled in the art of organic synthesis and include, but are not limited to, SOCl₂, POCl₃ and triflic anhydride.

The tenn "oxidize" refers to a synthetic transformation wherein a methyl group is converted to a carboxylic acid functionality. Such a transformation can be used, for example, to prepare compounds such as 10E, 11-C, 14E, 19E and 22D. Various reagents familiar to those skilled in the art of organic synthesis may be used to cany out this transformation including, but not limited to, KMnO in basic media (e.g., NaOH solution or aqueous pyridine) and K₂Cr₂O in acidic media (e.g., H₂SO₄).

The term "cyclize" refers to a synthetic transformation in which ortho-amino-benzoic acids, ortho-amino-benzoic esters, and ortho-amino-benzonitriles are converted to the conesponding 3H-Quinazolin-4-ones. Methods for effecting the cyclization of ortho-amino- benzonitriles include, but are not limited to, reaction with refluxing formic acid containing sodium acetate. Methods for effecting the cyclization of ortho-amino-benzoic acids include, but are not limited to, reaction with fonnamide at elevated temperatures or reaction with formamidine acetate in an inert solvent, also at elevated temperatures. Methods for effecting the cyclization of ortho-amino-benzoic esters include, but are not limited to, reaction with formamidine acetate at elevated temperature in an inert solvent.

In Scheme 8, "Η₂N-Prot" refers to a protected amino functionality, such as 4- methoxybenzylamine, and "deprotect" refers to a chemical method by which such a protecting group can be removed. For an overview of protection and deprotection methods as used by those skilled in the art of organic synthesis, see: Greene, T. and Wuts, P. Protective Groups in

Organic Synthesis, 3rd ed., John Wiley and Sons, 1999.

In Scheme 9, the term "nucleophile" refers to a primary or secondary amine, or an alkoxide.

In Scheme 19, the tenn "deprotection" refers to the process of cleaving the C-O bond of a benzylic ether to give a "deprotected" alcohol using various methods familiar to those who are skilled in the art of organic synthesis. This is exemplified in Scheme 19 in which compounds of general structure 191 can be converted to deprotected alcohols of general stracture 19J. Methods to effect this transformation include, but are not limited to, hydrogenolysis using hydrogen gas and an appropriate catalyst system such as palladium on carbon or Raney nickel. For an overview of protection and deprotection methods as used by those skilled in the art of organic synthesis, see: Greene, T. and Wuts, P. Protective Groups in Organic Synthesis, 3rd ed., John Wiley and Sons, 1999.

The term "demethylation" refers to the cleavage of the Me-O bond in a methyl ether fimctionality as exemplified by the conversion on 16D to 16E. This transformation can be carried out in a variety of ways familiar to those skilled in the art of organic synthesis including, but not limited to, treatment with HBr, treatment with Lewis acid/nucleophile combinations, Trimethylsilyl iodide, etc.

Scheme 1

1-B 1-C 1-D

Scheme 2

R-Ar-NH₂

Scheme 3

[M]-CN

3-E

Scheme 4

4-B 4-C 4-D

Base

4-E 4-A Scheme 5

5-B 5-C 5-D

Scheme 6

Scheme 7

7-B 7-C 7-A Scheme 8

or B(OH)₂

Scheme 9

KNCO

9-B 9-C 9-D

9-E 9-F 9-A

Scheme 10

Oxidize

10-B 10-C 10-D

Scheme 11

11-B 11-C 11-D 11-E

Reduce Cyclize

12-E 12-F 12-G

Activate

Scheme 13

A = CH,N 13-B 13-C 13-D

Scheme 14

B(OH)₂ Oxidize

14-B 14-C 14-D

14-E 14-F 14-G

Scheme 15

Reduce

15-B 15-C 15-D

(MeO)₃CCH₂CI Activate 15-E 15-F 15-G

Scheme 16

Demethylation Activate

16-E 16-F

16-G 16-H 16-1

16-J

16-K

Activate

Scheme 17

3) NH₄OAc/ACOH

POCI .

17-E 17-F

Scheme 18

or B(OH)₂ 18-D 18-B 18-C

18-E 18-A Scheme 19

B(OH)₂ Oxidize

19-B

19-E 19-F 19-G

19-H

Deprotection

Scheme 20

B(OH)₂ Oxidize

20-B 20-C 20-D

20-E 20-F 20-G

Activate

20-H 20-1

20-A

Scheme 21

Activate R-Ar-NH,

21 -B 21 -C

Scheme 22

Oxidize

22-B 22-C 22-D

22-E 22-A

Scheme 23

23-E 23-F

23-A

In certain embodiments, a VRl modulator may contain one or more asymmetric carbon atoms, so that the compound can exist in different stereoisomeric forms. Such forms can be, for example, racemates or optically active forms. As noted above, all stereoisomers are encompassed by the present invention. Nonetheless, it may be desirable to obtain single enantiomers (i.e., optically active forms). Standard methods for preparing single enantiomers include asymmetric synthesis and resolution of the racemates. Resolution of the racemates can be accomplished, for example, by conventional methods such as crystallization in the presence of a resolving agent, or chromatography using, for example a chiral HPLC column.

As noted above, the present invention encompasses pharmaceutically acceptable salts of the compounds described herein. As used herein, a "pharmaceutically acceptable salt" is an acid or base salt that is generally considered in the art to be suitable for use in contact with the tissues of human beings or animals without excessive toxicity, irritation, allergic response, or other problem or complication. Such salts include mineral and organic acid salts of basic residues such as amines, as well as alkali or organic salts of acidic residues such as carboxylic acids. Specific pharmaceutical salts include, but are not limited to, salts of acids such as hydrochloric, phosphoric, hydrobromic, malic, glycolic, fumaric, sulfuric, sulfamic, sulfanilic, formic, toluenesulfonic, methanesulfonic, benzene sulfonic, ethane disulfonic, 2-hydroxyethylsulfonic, nitric, benzoic, 2-acetoxybenzoic, citric, tartaric, lactic, stearic, salicylic, glutamic, ascorbic, pamoic, succinic, fumaric, maleic, propionic, hydroxymaleic, hydroiodic, phenylacetic, alkanoic such as acetic, HOOC-(CH₂)_n-COOH where n is 0-4, and the like. Similarly, phannaceutically acceptable cations include, but are not limited to sodium, potassium, calcium, aluminum, lithium and ammonium. Those of ordinary skill in the art will recognize further pharmaceutically acceptable salts for the compounds provided herein, including those listed by Remington's Pharmaceutical Sciences, 17th ed., Mack Publishing Company, Easton, PA, p. 1418 (1985). Accordingly, the present disclosure should be construed to include all pharmaceutically acceptable salts ofthe compounds specifically recited.

A wide variety of synthetic procedures are available for the preparation of pharmaceutically acceptable salts, hi general, a pharmaceutically acceptable salt can be synthesized from a parent compound that contains a basic or acidic moiety by any conventional chemical method. Briefly, such salts can be prepared by reacting the free acid or base forms of these compounds with a stoichiometric amount of the appropriate base or acid in water or in an organic solvent, or in a mixture of the two; generally, nonaqueous media like ether, ethyl acetate, ethanol, isopropanol, or acetonitrile are prefened.

Prodrugs of the compounds provided herein may be prepared by modifying functional groups present in the compounds in such a way that the modifications are cleaved to the parent compounds. Prodrugs include compounds wherein hydroxy, amine or sulfhydryl groups are bonded to any group that, when administered to a mammalian subject, cleaves to form a free hydroxyl, amino, or sulfhydryl group, respectively. Examples of prodrugs include, but are not limited to, acetate, formate and benzoate derivatives of alcohol and amine functional groups within the compounds provided herein. Preferred prodrugs include acylated derivatives. Those of ordinary skill in the art will recognize various synthetic methods that may be employed to prepare prodrugs ofthe compounds provided herein.

Compounds may be radiolabeled by canying out their synthesis using precursors comprising at least one atom that is a radioisotope. Each radioisotope is preferably carbon (e.g., ¹⁴C), hydrogen (e.g., ³H), sulfur (e.g., ³⁵S), or iodine (e.g., ¹²⁵I). Tritium labeled compounds may also be prepared catalytically via platinum-catalyzed exchange in tritiated acetic acid, acid- catalyzed exchange in tritiated trifluoroacetic acid, or heterogeneous-catalyzed exchange with tritium gas using the compound as substrate. In addition, certain precursors may be subjected to tritium-halogen exchange with tritium gas, tritium gas reduction of unsaturated bonds, or reduction using sodium borotritide, as appropriate. Preparation of radiolabeled compounds may be conveniently performed by a radioisotope supplier specializing in custom synthesis of radiolabeled probe compounds.

PHARMACEUTICAL COMPOSITIONS The present invention also provides pharmaceutical compositions comprising one or more VRl modulators, together with at least one physiologically acceptable carrier or excipient. Pharmaceutical compositions may comprise, for example, one or more of water, buffers (e.g., neutral buffered saline or phosphate buffered saline), ethanol, mineral oil, vegetable oil, dimethylsulfoxide, carbohydrates (e.g., glucose, mannose, sucrose or dextrans), mannitol, proteins, adjuvants, polypeptides or amino acids such as glycine, antioxidants, chelating agents such as EDTA or glutathione and/or preservatives. As noted above, other active ingredients may (but need not) be included in the pharmaceutical compositions provided herein.

Pharmaceutical compositions may be fonnulated for any appropriate manner of administration, including, for example, topical, oral, nasal, rectal or parenteral administration. The term parenteral as used herein includes subcutaneous, intradermal, intravascular (e.g., intravenous), intramuscular, spinal, intracranial, intrathecal and intraperitoneal injection, as well as any similar injection or infusion technique. In certain embodiments, compositions in a form suitable for oral use are prefened. Such forms include, for example, tablets, troches, lozenges, aqueous or oily suspensions, dispersible powders or granules, emulsion, hard or soft capsules, or syrups or elixirs. Within yet other embodiments, compositions of the present invention may be formulated as a lyophilizate. Formulation for topical administration may be prefened for certain conditions (e.g., in the treatment of skin conditions such as burns or itch).

Compositions intended for oral use may further comprise one or more components such as sweetening agents, flavoring agents, coloring agents and/or preserving agents in order to provide appealing and palatable preparations. Tablets contain the active ingredient in admixture with physiologically acceptable excipients that are suitable for the manufacture of tablets. Such excipients include, for example, inert diluents (e.g., calcium carbonate, sodium carbonate, lactose, calcium phosphate or sodium phosphate), granulating and disintegrating agents (e.g., com starch or alginic acid), binding agents (e.g., starch, gelatin or acacia) and lubricating agents (e.g., magnesium stearate, stearic acid or talc). The tablets may be uncoated or they may be coated by known techniques to delay disintegration and absorption in the gastrointestinal tract and thereby provide a sustained action over a longer period. For example, a time delay material such as glyceryl monosterate or glyceryl distearate may be employed. Formulations for oral use may also be presented as hard gelatin capsules wherein the active ingredient is mixed with an inert solid diluent (e.g., calcium carbonate, calcium phosphate or kaolin), or as soft gelatin capsules wherein the active ingredient is mixed with water or an oil medium (e.g., peanut oil, liquid paraffin or olive oil).

Aqueous suspensions contain the active material(s) in admixture with excipients suitable for the manufacture of aqueous suspensions. Such excipients include suspending agents (e.g., sodium carboxymethylcellulose, methylcellulose, hydropropylmethylcellulose, sodium alginate, polyvinylpynolidone, gum tragacanth and gum acacia); and dispersing or wetting agents (e.g., naturally-occunϊng phosphatides such as lecithin, condensation products of an alkylene oxide with fatty acids such as polyoxyethylene stearate, condensation products of ethylene oxide with long chain aliphatic alcohols such as heptadecaethyleneoxycetanol, condensation products of ethylene oxide with partial esters derived from fatty acids and a hexitol such as polyoxyethylene sorbitol monooleate, or condensation products of ethylene oxide with partial esters derived from fatty acids and hexitol anhydrides such as polyethylene sorbitan monooleate). Aqueous suspensions may also comprise one or more preservatives, for example ethyl, or n-propyl p- hydroxybenzoate, one or more coloring agents, one or more flavoring agents, and one or more sweetening agents, such as sucrose or saccharin.

Oily suspensions may be fonnulated by suspending the active ingredients in a vegetable oil (e.g., arachis oil, olive oil, sesame oil or coconut oil) or in a mineral oil such as liquid paraffin. The oily suspensions may contain a thickening agent such as beeswax, hard paraffin or cetyl alcohol. Sweetening agents such as those set forth above, and/or flavoring agents may be added to provide palatable oral preparations. Such suspensions may be preserved by the addition of an anti-oxidant such as ascorbic acid.

Dispersible powders and granules suitable for preparation of an aqueous suspension by the addition of water provide the active ingredient in admixture with a dispersing or wetting agent, suspending agent and one or more preservatives. Suitable dispersing or wetting agents and suspending agents are exemplified by those already mentioned above. Additional excipients, such as sweetening, flavoring and coloring agents, may also be present.

Phannaceutical compositions may also be in the form of oil-in-water emulsions. The oily phase may be a vegetable oil (e.g., olive oil or arachis oil), a mineral oil (e.g., liquid paraffin) or a mixture thereof. Suitable emulsifying agents include naturally-occurring gums (e.g., gum acacia or gum tragacanth), naturally-occurring phosphatides (e.g., soy bean, lecithin, and esters or partial esters derived from fatty acids and hexitol), anhydrides (e.g., sorbitan monoleate) and condensation products of partial esters derived from fatty acids and hexitol with ethylene oxide (e.g., polyoxyethylene sorbitan monoleate). An emulsion may also comprise one or more sweetening and/or flavoring agents.

Syrups and elixirs may be formulated with sweetening agents, such as glycerol, propylene glycol, sorbitol or sucrose. Such formulations may also comprise one or more demulcents, preservatives, flavoring agents and/or coloring agents. Formulations for topical administration typically comprise a topical vehicle combined with active agent(s), with or without additional optional components. Suitable topical vehicles and additional components are well known in the art, and it will be apparent that the choice of a vehicle will depend on the particular physical form and mode of delivery. Topical vehicles include water; organic solvents such as alcohols (e.g., ethanol or isopropyl alcohol) or glycerin; glycols (e.g., butylene, isoprene or propylene glycol); aliphatic alcohols (e.g., lanolin); mixtures of water and organic solvents and mixtures of organic solvents such as alcohol and glycerin; lipid-based materials such as fatty acids, acylglycerols (including oils, such as mineral oil, and fats of natural or synthetic origin), phosphoglycerides, sphingolipids and waxes; protein-based materials such as collagen and gelatin; silicone-based materials (both non-volatile and volatile); and hydrocarbon-based materials such as microsponges and polymer matrices. A composition may further include one or more components adapted to improve the stability or effectiveness of the applied formulation, such as stabilizing agents, suspending agents, emulsifying agents, viscosity adjusters, gelling agents, preservatives, antioxidants, skin penetration enhancers, moisturizers and sustained release materials. Examples of such components are described in Martindale~The Extra Pharmacopoeia (Pharmaceutical Press, London 1993) and Martin (ed.), Remington's Pharmaceutical Sciences. Formulations may comprise microcapsules, such as hydroxymethylcellulose or gelatin-microcapsules, liposomes, albumin microspheres, microemulsions, nanoparticles or nanocapsules. A topical formulation may be prepared in a variety of physical forms including, for example, solids, pastes, creams, foams, lotions, gels, powders, aqueous liquids and emulsions. The physical appearance and viscosity of such forms can be governed by the presence and amount of emulsifier(s) and viscosity adjuster(s) present in the fonnulation. Solids are generally firm and non-pourable and commonly are formulated as bars or sticks, or in particulate form; solids can be opaque or transparent, and optionally can contain solvents, emulsifiers, moisturizers, emollients, fragrances, dyes/colorants, preservatives and other active ingredients that increase or enhance the efficacy of the final product. Creams and lotions are often similar to one another, differing mainly in their viscosity; both lotions and creams may be opaque, translucent or clear and often contain emulsifiers, solvents, and viscosity adjusting agents, as well as moisturizers, emollients, fragrances, dyes/colorants, preservatives and other active ingredients that increase or enhance the efficacy ofthe final product. Gels can be prepared with a range of viscosities, from thick or high viscosity to thin or low viscosity. These formulations, like those of lotions and creams, may also contain solvents, emulsifiers, moisturizers, emollients, fragrances, dyes/colorants, preservatives and other active ingredients that increase or enhance the efficacy of the final product. Liquids are thinner than creams, lotions, or gels and often do not contain emulsifiers. Liquid topical products often contain solvents, emulsifiers, moisturizers, emollients, fragrances, dyes/colorants, preservatives and other active ingredients that increase or enhance the efficacy ofthe final product. Suitable emulsifiers for use in topical formulations include, but are not limited to, ionic emulsifiers, cetearyl alcohol, non-ionic emulsifiers like polyoxyethylene oleyl ether, PEG-40 stearate, ceteareth-12, ceteareth-20, ceteareth-30, ceteareth alcohol, PEG- 100 stearate and glyceryl stearate. Suitable viscosity adjusting agents include, but are not limited to, protective colloids or non-ionic gums such as hydroxyethylcellulose, xanthan gum, magnesium aluminum silicate, silica, microcrystalline wax, beeswax, paraffin, and cetyl palmitate. A gel composition may be formed by the addition of a gelling agent such as chitosan, methyl cellulose, ethyl cellulose, polyvinyl alcohol, polyquaterniums, hydroxyethylcellulose, hydroxypropylcellulose, hydroxypropylmethylcellulose, carbomer or ammoniated glycynhizinate. Suitable surfactants include, but are not limited to, nonionic, amphoteric, ionic and anionic surfactants. For example, one or more of dimethicone copolyol, polysorbate 20, polysorbate 40, polysorbate 60, polysorbate 80, lauramide DEA, cocamide DEA, and cocamide MEA, oleyl betaine, cocamidopropyl phosphatidyl PG-dimonium chloride, and ammonium laureth sulfate may be used within topical formulations. Suitable preservatives include, but are not limited to, antimicrobials such as methylparaben, propylparaben, sorbic acid, benzoic acid, and formaldehyde, as well as physical stabilizers and antioxidants such as vitamin E, sodium ascorbate/ascorbic acid and propyl gallate. Suitable moisturizers include, but are not limited to, lactic acid and other hydroxy acids and their salts, glycerin, propylene glycol, and butylene glycol. Suitable emollients include lanolin alcohol, lanolin, lanolin derivatives, cholesterol, petrolatum, isostearyl neopentanoate and mineral oils. Suitable fragrances and colors include, but are not limited to, FD&C Red No. 40 and FD&C Yellow No. 5. Other suitable additional ingredients that may be included a topical formulation include, but are not limited to, abrasives, absorbents, anti-caking agents, anti-foaming agents, anti-static agents, astringents (e.g., witch hazel, alcohol and herbal extracts such as chamomile extract), binders/excipients, buffering agents, chelating agents, film forming agents, conditioning agents, propellants, opacifying agents, pH adjusters and protectants.

An example of a suitable topical vehicle for formulation of a gel is: hydroxypropylcellulose (2.1%); 70/30 isopropyl alcohol/water (90.9%); propylene glycol (5.1%); and Polysorbate 80 (1.9%). An example of a suitable topical vehicle for formulation as a foam is: cetyl alcohol (1.1%); stearyl alcohol (0.5%; Quatemium 52 (1.0%); propylene glycol (2.0%); Ethanol 95 PGF3 (61.05%); deionized water (30.05%); P75 hydrocarbon propellant (4.30%). All percents are by weight.

Typical modes of delivery for topical compositions include application using the fingers; application using a physical applicator such as a cloth, tissue, swab, stick or brash; spraying (including mist, aerosol or foam spraying); dropper application; sprinkling; soaking; and rinsing. Controlled release vehicles can also be used.

A pharmaceutical composition may be prepared as a sterile injectible aqueous or oleaginous suspension. The modulator, depending on the vehicle and concentration used, can either be suspended or dissolved in the vehicle. Such a composition may be formulated according to the known art using suitable dispersing, wetting agents and/or suspending agents such as those mentioned above. Among the acceptable vehicles and solvents that may be employed are water, 1,3-butanediol, Ringer's solution and isotonic sodium chloride solution. In addition, sterile, fixed oils may be employed as a solvent or suspending medium. For this purpose any bland fixed oil may be employed, including synthetic mono- or diglycerides. In addition, fatty acids such as oleic acid find use in the preparation of injectible compositions, and adjuvants such as local anesthetics, preservatives and/or buffering agents can be dissolved in the vehicle.

Modulators may also be prepared in the form of suppositories (e.g., for rectal administration). Such compositions can be prepared by mixing the drag with a suitable non- irritating excipient that is solid at ordinary temperatures but liquid at the rectal temperature and will therefore melt in the rectum to release the drag. Suitable excipients include, for example, cocoa butter and polyethylene glycols.

Pharmaceutical compositions maybe formulated as sustained release formulations (i.e., a formulation such as a capsule that effects a slow release of modulator following administration). Such formulations may generally be prepared using well known technology and administered by, for example, oral, rectal or subcutaneous implantation, or by implantation at the desired target site. Carriers for use within such formulations are biocompatible, and may also be biodegradable; preferably the formulation provides a relatively constant level of modulator release. The amount of modulator contained within a sustained release formulation depends upon, for example, the site of implantation, the rate and expected duration of release and the nature ofthe condition to be treated or prevented.

In addition to or together with the above modes of administration, a modulator may be conveniently added to food or drinking water (e.g., for administration to non-human animals including companion animals (such as dogs and cats) and livestock). Animal feed and drinking water compositions may be formulated so that the animal takes in an appropriate quantity ofthe composition along with its diet. It may also be convenient to present the composition as a premix for addition to feed or drinking water.

Modulators are generally administered in a capsaicin receptor modulatory amount (i.e., an amount that achieves a concentration in a body fluid (e.g., blood, plasma, serum, CSF, synovial fluid, lymph, cellular interstitial fluid, tears or urine) that is sufficient to inhibit the binding of vanilloid ligand to VRl in vitro). A dose is considered to be therapeutically effective if it results in a discernible patient benefit, such as pain relief, as described herein. Prefened systemic doses are no higher than 50 mg per kilogram of body weight per day (e.g., ranging from about 0.001 mg to about 50 mg per kilogram of body weight per day), with oral doses generally being about 5-20 fold higher than intravenous doses (e.g., ranging from 0.01 to 40 mg per kilogram of body weight per day).

The amount of active ingredient that may be combined with the carrier materials to produce a single dosage form will vary depending, for example, upon the patient being treated and the particular mode of administration. Dosage unit forms will generally contain between from about 10 μg to about 500 mg of an active ingredient. Optimal dosages may be established using routine testing, and procedures that are well known in the art.

Pharmaceutical compositions may be packaged for treating conditions responsive to VRl modulation (e.g., treatment of exposure to vanilloid ligand, pain, itch, obesity or urinary incontinence). Packaged pharmaceutical compositions may include a container holding a therapeutically effective amount of at least one VRl modulator as described herein and instractions (e.g., labeling) indicating that the contained composition is to be used for treating a condition responsive to VRl modulation in the patient. METHODS OF USE

VRl modulators provided herein may be used as agonists or (preferably) antagonists of capsaicin receptors in a variety of contexts, both in vitro and in vivo. Within certain aspects, VRl antagonists may be used to inhibit the binding of vanilloid ligand agonist (such as capsaicin and/or RTX) to capsaicin receptor in vitro or in vivo. In general, such methods comprise the step of contacting a capsaicin receptor with a sufficient amount of one or more quinazolin-4-ylamine analogues, in the presence of vanilloid ligand in aqueous solution and under conditions otherwise suitable for binding of the ligand to capsaicin receptor. The capsaicin receptor may be present in solution or suspension (e.g., in an isolated membrane or cell preparation), or in a cultured or isolated cell. Within certain embodiments, the capsaicin receptor is expressed by a neuronal cell present in a patient, and the aqueous solution is a body fluid, hi general, the amount of qumazolin-4-ylamine analogue(s) contacted with the receptor should yield a concentration in the aqueous solution sufficient to inhibit vanilloid ligand binding to VRl in vitro as measured, for example, using a binding assay as described in Example 5 and/or a calcium mobilization assay as described in Example 6. Preferably, one or more quinazolin-4-ylamine analogues are administered to an animal in an amount such that the analogue is present in at least one body fluid of the animal at a therapeutically effective concentration that is 100 nanomolar or less, preferably 50 nanomolar or less, 20 nanomolar or less, or 10 nanomolar or less. For example, such compounds maybe administered at a dose that is less than 20 mg/kg body weight, preferably less than 5 mg/kg and, in some instances, less than 1 mg/kg.

Also provided herein are methods for modulating, preferably inhibiting, the signal- transducing activity of a capsaicin receptor. Such modulation may be achieved by contacting a capsaicin receptor (either in vitro or in vivo) with an effective amount of one or more VRl modulators provided herein under conditions suitable for binding of the modulator(s) to the receptor. The receptor may be present in solution or suspension, in a cultured or isolated cell preparation or within a patient. Modulation of signal tranducing activity may be assessed by detecting an effect on calcium ion conductance (also refened to as calcium mobilization or flux), hi general, an effective amount of VRl modulator(s) is an amount sufficient to yield a concentration (in an aqueous solution that is in contact with the receptor) that is sufficient to modulate VRl signal transducing activity in vitro within a calcium mobilization assay as described in Example 6. VRl modulator(s) provided herein are preferably administered to a patient (e.g., a human) orally or topically, and are present within at least one body fluid of the animal while modulating VRl signal-transducing activity. Prefened VRl modulators for use in such methods modulate VRl signal-transducing activity in vitro at a concentration of 1 nanomolar or less, preferably 100 picomolar or less, more preferably 20 picomolar or less, and in vivo at a concentration of 100 nanomolar or less in a body fluid such as blood.

The present invention further provides methods for treating conditions responsive to VRl modulation. Within the context ofthe present invention, the term "treatment" encompasses both disease-modifying treatment and symptomatic treatment, either of which may be prophylactic (i.e., before the onset of symptoms, in order to prevent, delay or reduce the severity of symptoms) or therapeutic (i.e., after the onset of symptoms, in order to reduce the severity and/or duration of symptoms). A condition is "responsive to VRl modulation" if it is characterized by inappropriate activity of a capsaicin receptor, regardless of the amount of vanilloid ligand present locally, and/or if modulation of capsaicin receptor activity results in alleviation of the condition or a symptom thereof. Such conditions include, for example, symptoms resulting firom exposure to VRl -activating stimuli, pain, respiratory disorders such as asthma and chronic obstructive pulmonary disease, itch, urinary incontinence, cough, hiccup, and obesity, as described in more detail below. Such conditions may be diagnosed and monitored using criteria that have been established in the art. Patients may include humans, domesticated companion animals and livestock, with dosages as described above.

Treatment regimens may vary depending on the compound used and the particular condition to be treated. However, for treatment of most disorders, a frequency of administration of 4 times daily or less is prefened. In general, a dosage regimen of 2 times daily is more prefened, with once a day dosing particularly prefened. For the treatment of acute pain, a single dose that rapidly reaches effective concentrations is desirable. It will be understood, however, that the specific dose level and treatment regimen for any particular patient will depend upon a variety of factors including the activity of the specific compound employed, the age, body weight, general health, sex, diet, time of administration, route of administration, and rate of excretion, drag combination and the severity of the particular disease undergoing therapy, hi general, the use of the minimum dose sufficient to provide effective therapy is prefened. Patients may generally be monitored for therapeutic effectiveness using medical or veterinary criteria suitable for the condition being treated or prevented. Patients experiencing symptoms resulting from exposure to capsaicin receptor-activating stimuli include individuals with bums caused by heat, light, tear gas or acid and those whose mucous membranes are exposed (e.g., via ingestion, inhalation or eye contact) to capsaicin (e.g., from hot peppers or in pepper spray) or a related irritant such as acid, tear gas or air pollutants. The resulting symptoms (which may be treated using compounds provided herein) may include, for example, pain, broncho-constriction and inflammation.

Pain that may be treated using the compounds provided herein may be chronic or acute and includes, but is not limited to, peripheral nerve-mediated pain (especially neuropathic pain). Compounds provided herein may be used in the treatment of, for example, postmastectomy pain syndrome, stump pain, phantom limb pain, oral neuropathic pain, toothache (dental pain), denture pain, postherpetic neuralgia, diabetic neuropathy, reflex sympathetic dystrophy, trigeminal neuralgia, osteoarthritis, rheumatoid arthritis, fibromyalgia, Guillain-Bane syndrome, meralgia paresthetica, buming-mouth syndrome and/or bilateral peripheral neuropathy. Additional neuropathic pain conditions include causalgia (reflex sympathetic dystrophy - RSD, secondary to injury of a peripheral nerve), neuritis (including, for example, sciatic neuritis, peripheral neuritis, polyneuritis, optic neuritis, postfebrile neuritis, migrating neuritis, segmental neuritis and Gombault's neuritis), neuronitis, neuralgias (e.g., those mentioned above, cervicobrachial neuralgia, cranial neuralgia, geniculate neuralgia, glossopharyngial neuralgia, migranous neuralgia, idiopathic neuralgia, intercostals neuralgia, mammary neuralgia, mandibular joint neuralgia, Morton's neuralgia, nasocihary neuralgia, occipital neuralgia, red neuralgia, Sluder's neuralgia, splenopalatine neuralgia, supraorbital neuralgia and vidian neuralgia), surgery-related pain, musculoskeletal pain, AIDS-related neuropathy, MS-related neuropathy, and spinal cord injury-related pain. Headache, including headaches involving peripheral nerve activity, such as sinus, cluster (i.e., migranous neuralgia) and some tension and headaches and migraine, may also be treated as described herein. For example, migraine headaches may be prevented by administration of a compound provided herein as soon as a pre- migrainous aura is experienced by the patient. Further pain conditions that can be treated as described herein include "burning mouth syndrome," labor pains, Charcot's pains, intestinal gas pains, menstrual pain, acute and chronic back pain, hemonhoidal pain, dyspeptic pains, angina, nerve root pain, homotopic pain and heterotopic pain - including cancer associated pain (e.g., in patients with bone cancer), pain (and inflammation) associated with venom exposure (e.g., due to snake bite, spider bite, or insect sting) and trauma associated pain (e.g., post-surgical pain, pain from cuts, braises and broken bones, and bum pain). Additional pain conditions that may be treated as described herein include pain associated with inflammatory bowel disease, irritable bowel syndrome and/or inflammatory bowel disease.

Within certain aspects, VRl antagonists including (but not limited to) those specifically recited herein, may be used for the treatment of mechanical pain. As used herein, the term "mechanical pain" refers to pain other than headache pain that is not neuropathic or a result of exposure to heat, cold or external chemical stimuli. Mechanical pain includes physical trauma (other than thermal or chemical burns or other irritating and/or painful exposures to noxious chemicals) such as post-surgical pain and pain from cuts, bruises and broken bones; toothache, denture pain; nerve root pain; osteoartiritis; rheumatoid arthritis; fibromyalgia; meralgia paresthetica; back pain; cancer-associated pain; angina; carpel tunnel syndrome; and pain resulting from bone fracture, labor, hemonhoids, intestinal gas, dyspepsia, and menstruation. Any VRl antagonist that binds to VRl with a Ki of less than 100 μM and/or inhibits VRl activity with an EC₅₀ of less than or equal to 100 μM (determined as described herein) may be used. Preferably, the VRl antagonist used is not a capsaicin analogue; particularly prefened VRl antagonists are those provided herein.

Itching conditions that may be treated include psoriatic pruritis, itch due to hemodialysis, aguagenic pruritus, and itching associated with vulvar vestibulitis, contact dermatitis, insect bites and skin allergies. Urinary incontinence, as used herein, includes detrasor hyperflexia of spinal origin and bladder hypersensitivity, both of which may be treated as described herein. Compounds provided herein may also be used as anti-tussive agents (to prevent, relieve or suppress coughing) and for the treatment of hiccup, and to promote weight loss in an obese patient. Therapeutically effective amounts for use in such methods are generally sufficient to provide detectable relief from the condition being treated.

Within other aspects, VRl antagonists provided herein may be used within combination therapy for the treatment of conditions involving inflammatory components. Such conditions include, for example, autoimmune disorders and pathologic autoimmune responses known to have an inflammatory component including, but not limited to, arthritis (especially rheumatoid arthritis), psoriasis, Crohn's disease, lupus erythematosus, irritable bowel syndrome, tissue graft rejection, and hyperacute rejection of transplanted organs. Other such conditions include trauma (e.g., injury to the head or spinal cord), cardio- and cerebo-vascular disease and certain infectious diseases.

Within such combination therapy, a VRl antagonist is administered to a patient along with an anti-inflammatory agent. The VRl antagonist and anti-inflammatory agent may be present in the same pharmaceutical composition, or may be administered separately in either order. Anti-inflammatory agents include, for example, non-steroidal anti-inflammatory drugs (NSAEDs), non-specific and cyclooxygenase-2 (COX-2) specific cyclooxgenase enzyme inhibitors, gold compounds, corticosteroids, methotrexate, tumor necrosis factor (TNF) receptor antagonists, anti-TNF alpha antibodies, anti-C5 antibodies, and interleukin- 1 (IL-1) receptor antagonists. Examples of NSAIDs include, but are not limited to ibuprofen (e.g., ADVIL™, MOTRTN™), flurbiprofen (ANSAID™), naproxen or naproxen sodium (e.g., NAPROSYN, ANAPROX, ALEVE™), diclofenac (e.g., CATAFLAM™, VOLTAREN™), combinations of diclofenac sodium and misoprostol (e.g., ARTHROTEC™), sulindac (CLINORIL™), oxaprozin (DAYPRO™), diflunisal (DOLOBID™), piroxicam (FELDENE™), indomethacin (ΓNDOCIN™), etodolac (LODINE™), fenoprofen calcium (NALFON™), ketoprofen (e.g., ORUDIS™, ORUVAIL™), sodium nabumetone (RELAFEN™), sulfasalazine (AZULFIDINE™), tolmetin sodium (TOLECTIN™), and hydroxychloroquine (PLAQUENIL™). A particular class of NSAIDs consists of compounds that inhibit cyclooxygenase (COX) enzymes, such as celecoxib (CELEBREX™) and rofecoxib (NIOXX™). NSAIDs further include sahcylates such as acetylsahcylic acid or aspirin, sodium salicylate, choline and magnesium sahcylates (TRILISATE™), and salsalate (DISALCID™), as well as corticosteroids such as cortisone (CORTONE™ acetate), dexamethasone (e.g., DECADRON™), methylprednisolone (MEDROL™) prednisolone (PRELONE™), prednisolone sodium phosphate (PEDIAPRED™), and prednisone (e.g., PREDNICEN-M™, DELTASONE™, STERAPRED™).

Suitable dosages for VRl antagonist within such combination therapy are generally as described above. Dosages and methods of administration of anti-inflammatory agents can be found, for example, in the manufacturer's instructions in the Physician 's Desk Reference. In certain embodiments, the combination administration of a NR1 antagonist with an anti- inflammatory agent results in a reduction of the dosage of the anti-inflammatory agent required to produce a therapeutic effect. Thus, preferably, the dosage of anti-inflammatory agent in a combination or combination treatment method of the invention is less than the maximum dose advised by the manufacturer for administration of the anti-inflammatory agent without combination administration of a NR1 antagonist. More preferably this dosage is less than ³A, even more preferably less than V_, and highly preferably, less than ^l of the maximum dose, while most preferably the dose is less than 10% of the maximum dose advised by the manufacturer for administration of the anti-inflammatory agent(s) when aclministered without combination administration of a NR1 antagonist. It will be apparent that the dosage amount of VRl antagonist component of the combination needed to achieve the desired effect may similarly be affected by the dosage amount and potency of the anti-inflammatory agent component ofthe combination.

In certain prefened embodiments the combination administration of a NR1 antagonist with an anti-inflammatory agent is accomplished by packaging one or more NR1 antagonists and one or more anti-inflammatory agents in the same package, either in separate containers within the package or preferably as a mixture of one or more NR1 antagonists and one or more anti-inflammatory agents. Prefened mixtures are formulated for oral administration (e.g., as pills, capsules, tablets or the like). Preferably the package comprises a label bearing indicia indicating that the one or more NR1 antagonists and one or more anti-inflammatory agents are to be taken together for the treatment of an inflammatory pain condition. A highly prefened combination is one in which the anti-inflammatory agent(s) include at least one COX-2 specific cyclooxgenase enzyme inhibitor such as valdecoxib (BEXTRA®), lumiracoxib (PREXIGE™), etoricoxib (ARCOXIA®), celecoxib (CELEBREX®) and/or rofecoxib (NIOXX®).

The methods discussed above generally employ modulators that are NR1 antagonists; however, methods are also provided herein that employ modulators that are VRl agonists. Such modulators may be used, for example, in crowd control (as a substitute for tear gas) or personal protection (e.g., in a spray formulation) or as pharmaceutical agents for the treatment of pain, itch or urinary incontinence via capsaicin receptor desensitization. hi general, compounds for use in crowd control or personal protection are formulated and used according to conventional tear gas or pepper spray technology.

Within separate aspects, the present invention provides a variety of non-pharmaceutical in vitro and in vivo uses for the compounds provided herein. For example, such compounds may be labeled and used as probes for the detection and localization of capsaicin receptor (in samples such as cell preparations or tissue sections, preparations or fractions thereof). Compounds may also be used as positive controls in assays for receptor activity, as standards for determining the ability of a candidate agent to bind to capsaicin receptor, or as radiotracers for positron emission tomography (PET) imaging or for single photon emission computerized tomography (SPECT). Such methods can be used to characterize capsaicin receptors in living subjects. For example, a VRl modulator may be labeled using any of a variety of well known techniques (e.g., radiolabeled with a radionuclide such as tritium, as described herein), and incubated with a sample for a suitable incubation time (e.g., determined by first assaying a time course of binding). Following incubation, unbound compound is removed (e.g., by washing), and bound compound detected using any method suitable for the label employed (e.g., autoradiography or scintillation counting for radiolabeled compounds; spectroscopic methods may be used to detect luminescent groups and fluorescent groups). As a control, a matched sample containing labeled compound and a greater (e.g., 10-fold greater) amount of unlabeled compound may be processed in the same manner. A greater amount of detectable label remaining in the test sample than in the control indicates the presence of capsaicin receptor in the sample. Detection assays, including receptor autoradiography (receptor mapping) of capsaicin receptor in cultured cells or tissue samples may be performed as described by Kuhar in sections 8.1.1 to 8.1.9 of Cunent Protocols in Pharmacology (1998) John Wiley & Sons, New York.

Modulators provided herein may also be used within a variety of well known cell separation methods. For example, modulators may be linked to the interior surface of a tissue culture plate or other support, for use as affinity ligands for immobilizing and thereby isolating, capsaicin receptors (e.g., isolating receptor-expressing cells) in vitro. Within one prefened embodiment, a modulator linked to a fluorescent marker, such as fluorescein, is contacted with the cells, which are then analyzed (or isolated) by fluorescence activated cell sorting (FACS). The following Examples are offered by way of illustration and not by way of limitation.

Unless otherwise specified all reagents and solvent are of standard commercial grade and are used without further purification.

EXAMPLES

EXAMPLE 1 Preparation of Representative Compounds This Example illustrates the preparation of representative substituted quinazolin-4-ylamine analogues. Mass spectroscopy data shown in this and subsequent Examples is Electrospray MS, obtained in positive ion mode with a 15V cone voltage, using a Micromass Time-of-Flight LCT, equipped with a Waters 600 pump, Waters 996 photodiode array detector, Gilson 215 autosampler, and a Gilson 841 microinjector. MassLynx (Advanced Chemistry Development, Inc; Toronto, Canada) version 3.5 software was used for data collection and analysis. Sample volume of 1 microliter was injected onto a 50x4.6mm Chromolith SpeedROD C18 column, and eluted using a 2-phase linear gradient at 6ml/min flow rate. Sample was detected using total absorbance count over the 220-340nm UN range. The elution conditions were: Mobile Phase A- 95/5/0.1 Water/Methanol/TFA; Mobile Phase B-5/95/0.05 Water/Methanol/TFA. Gradient- Time.min) "/ol¬

0 io

0.5 100

1.2 100

1.21 10 The total run time was 2 minutes inject to inject.

A. (4-Trifluoromethyl-phenyl)-r7-(2-trifluoromethyl-phenyl)-quinazolm-4-yl1-amine (Cmpd 1)

1. 3-Nitro-2 '-trifluoromethyl-biphenyl-4-carboxylic acid methyl ester

To a solution of 2-(trifluoromemyl)-phenylboronic acid (4.4 g, 0.0232 mol), 2-

(dicyclohexylphosphino)biphenyl (111 mg, 0.318 mmol), and potassium phosphate (6.52 g, 0.031 mmol) in toluene, add palladium (II) acetate (36 mg, 0.160 mmol). Purge the reaction mixture for 10 minutes with dry nitrogen and then add 4-chloro-2-nitrobenzoic acid methyl ester. Heat the stirring reaction mixture overnight at 80°C, cool the mixture and filter through celite using ethyl acetate. Concentrate under reduced pressure, take up in fresh ethyl acetate and wash the solution with NaHCO₃ (saturated aqueous). Dry the solution (Na₂S0₄), concentrate under reduced pressure and then filter through a pad of silica gel using ethyl acetate as eluent. Removal of solvent under reduced pressure gives pure 3-nitro-2'- trifluoromethyl-biphenyl-4-carboxylic acid methyl ester as an oil. 2. 3-amino-2 '-trifluorornethyl-biphenyl-4-carboxylic acid methyl ester

In a Parr apparatus, hydrogenate an ethanolic solution of 3-nitro-2'-trifluoromethyl-biphenyl-4- carboxylic acid methyl ester (5.54 g, 0.0169 mol) under 55 psi of hydrogen using tetrakis(triphenylphosphine)palladium (0) (300 mg). After 18 hours, filter the mixture through celite and concentrate under reduced pressure to give 3-amino-2'-trifluoromethyl-biphenyl-4-carboxylic acid methyl ester as a solid.

3. 7-(2-Trifluoromethyl-phenyl)-3H-quinazolin-4-one

Heat a solution of 3-amino-2'-trifluoromethyl-biphenyl-4-carboxylic acid methyl ester (5.0 g,

0.0169 mol) and formamidine acetate (2.8 g, 0.0203 mol) in 2-methoxyethanol at reflux for 8 hours. Cool the mixture and concentrate under reduced pressure to give a dark oil. Dissolve the residue in 10% NaOH and wash the aqueous with ether (3X). Bring the aqueous layer to pH ~4 using 12N HCl to produce a milky solution. Extract the solution with EtOAc, wash the EtOAc with brine, dry (Na₂SO₄) and concentrate under reduced pressure to give 7-(2-Trifluoromethyl-phenyl)-3i_^"-quinazolin-4-one as a beige solid.

4. 4-chloro-7-(2-trifluorometlιyl-ph

Reflux a solution of 7-(2-Trifluoromethyl-phenyl)-3i7-quinazolin-4-one (1.12 g, 0.0039 mol) in POCl₃ for 16 hours. Cool the mixture and concentrate under reduced pressure. Partition the residue between saturated aqueous NaHCO₃ and EtOAc. Wash the EtOAc layer once with additional NaHC0₃, dry it (Na₂SO₄), and concentrate under reduced pressure to obtain the crude product as a solid. Filter the residue through a 2 inch pad of silica gel (50% EtOAc/Hexanes) and concentrate under reduce pressure to give 4-chloro-7-(2-trifluoromethyl-phenyl)-quinazoline as a pale yellow-brown solid. 5. (4-Trifluo7'omethyl-phenyl)-[7-(2-trifluoromethyl-phenyl)-quinazolin-4-yl]-amine

Reflux a solution of 4-chloro-7-(2-trifluoromethyl-phenyl)-quinazoline (258 mg, 0.836 mmol) and 4-(trifluoromethyl)-aniline (269 mg, 1.67 mmol) in isopropyl alcohol for 8 hours. Cool the solution, collect the precipitate via filtration and wash with dry ether (3x) to give pure (4-Trifluoromethyl-phenyl)- [7-(2-trifluoromethyl-phenyl)-quinazolin-4-yl]-amine as the mono-HCl salt. Mass spec. 433.1.

B. (4-tert-Butyl-phenyl)-r7-(2-trifluoromethyl-phenyl)-quinolin-4-yll -amine (Cmpd 2) 1. 7-(2-Trifluoromethyl-phenyl)-quinolin-4-ol

Combine 7-chloroquinolin-4-ol (1000 mg, 5.55 mmol,) 2-(trifluoromethyl)phenylboronic acid (1583 mg, 8.33 mmol) and toluene (50 mL), and bubble nitrogen into the solution for 10 minutes. Add palladium acetate (25 mg, 0.11 mmol), 2-(dicyclohexylphosphino)biphenyl (78 mg, 0.22 mmol), and K₃PO₄ (2353 mg, 11.1 mmol) and heat at 90°C for 16 hours. Let cool, add water (25 mL) and EtOAc (50 mL), and remove any insoluble material by filtration. Separate the EtOAc layer, and extract the aqueous layer twice with EtOAc (25 mL each). Combine the EtOAc extracts, dry (Na₂SO₄), and evaporate. Purify by silica gel chromatography (94% CH2C12/ 5% MeOH/ 1% NH₄OH) to provide 110 mg of 7-(2-Trifluoromethyl-phenyl)-quinolin-4-ol as a white solid.

2. 4-Chloro- 7-(2-trifluoromethyl-phenyl)-quinoline

Heat a mixture of 7-(2-trifluoromethyl-phenyl)-quinolin-4-ol (50 mg, 0.17 mmol) in

POCI3 (10 mL) at 90°C for 16 hours. Evaporate the POCl₃, and add ice (100 g) followed by careful addition of saturated NaHCO₃. Extract with EtOAc, dry (Na₂SO₄), and evaporate to provide 4-chloro-7- (2-trifluoromethyl-phenyl)-quinoline as a tan solid. 3. (4-tert-Butyl-phenyl)-[7-(2-trifluoromethyl-phenyl)-quinolin-4-yl]-amine

61

Heat a mixture of 4-chloro-7-(2-trifluoromethyl-phenyl)-quinoline (42 mg, 0.14 mmol) and 4- (tert-butyl)amline (41 mg, 0.29 mmol) in 2-propanol (10 mL) at reflux for 3 hours. Evaporate the mixture, add IM NaOH (10 mL), extract twice with EtOAc (10 mL each), dry (Na₂SO₄), and evaporate to provide the crude product. Purify by silica gel chromatography, eluting with 75% hexane-EtOAc to provide (4-tert-Butyl-phenyl)-[7-(2-trifluoromethyl-phenyl)-quinolin-4-yl] -amine as a white solid. Mass spec. 420.2.

C. (4-tgrt-Butyl-phenyl)-[7-(2-trifluoromethyl-phenyl)-pyrido 3.2-dlpyrimidin-4-yll-amine (cmpd 3) 1. 5-bromo-3-nitropyridine-2-carbonitrile

Heat a solution of 2,5-dibromo-3-nitropyridine (1.77g, 6.3 mmol; Malinowski (1988) Bull. Soc. Chim. Belg. 97:51; see also US 5,801,183) and cuprous cyanide (0.60 g, 6.69 mmol) in N,N- dimethylacetamide (25 mL) at 100°C for 72 hours. After cooling, dilute the mixture with water (25 mL) and extract twice with EtOAc (25 mL each), then wash twice with water (25 mL each). The combined EtOAc extracts are dried (Na₂SO₄), evaporated, and purified by flash chromatography (50% EtOAc- hexane) to obtain 5-bromo-3-nitropyridine-2-carbonitrile as a pale solid.

2. 3-Amino-5-bromopyridine-2-carbonitrile

Mix 5-bromo-3-nitropyridine-2-carbonitrile (1.5g, 5.3 mmol) and SnCl₂-dihydrate

(5.00g, 26.3 mmol) in concentrated HCl and allow to stir at room temperature overnight. Work up by adding ice and carefully adding 10 M NaOH until basic. Extract twice with Et₂O (200 mL), dry (Na₂SO₄) and evaporate. Purify by silica gel chromatography (75% hexane-EtOAc) to furnish 3-amino- 5-bromopyridine-2-carbonitrile as a pale solid. 3. 7-Bromopyrido[3,2-d]pyrimidin-4-ol

Reflux a mixture of 3-amino-5-bromopyridine-2-carbonitrile (504 mg, 2.00 mmol) and sodium acetate (312 mg, 3.81 mmol) in formic acid (20 mL) for 16 hours. Work up by evaporating to a white solid, and add 3N NaOH (50 mL). Filter off any undissolved material, then re-form the free pyrimidinol by adding concentrated HCl until a pH of 3 is achieved. Collect 7-Bromopyrido[3,2-t]pyrimidin-4-ol and let dry overnight.

4. Bromo-4-chloropyrido[3-2-d] pyrimidine

Heat a mixture of 7-Bromopyrido[3,2-t/]pyrimidin-4-ol (35 mg, 0.15 mmOl) and POCL₃ (10 mL) at 90°C for 16 hours. Evaporate the POCl₃, and add ice (100 g) followed by careful addition of saturated NaHCO₃. Extract twice with EtOAc, dry (Na₂SO₄), and evaporate to provide bromo-4- chloropyrido[3-2-c]pyrimidine as a white solid.

5. (7-Bromo-pyrido[3,2-d]pyrimidin-4-yl)-4-tert-butyl-phenyl)-amine

Heat a mixture of bromo-4-chloropyrido[3-2-G?]pyrimidine (35 mg, 0.14 mmol) and 4-(tert- butyl)aniline (43 mg, 0.29 mmol) in 2-propanol (10 mL) at reflux for 3 hours. Evaporate the mixture, add IM NaOH (10 mL), extract twice with EtOAc (10 mL each), dry (Na2SO4), and evaporate to provide the crude product. Purify by silica gel chromatography, eluting with 75% hexane-EtOAc to provide (7-bromo-pyrido[3,2-(f]pyrimidin-4-yl)-4-tert-butyl-phenyl)-amine as a white solid.

6. (4-tert-Butyl-plιenyl)-[7-(2-trifluoromethyl-phenyl)-pyrido[3,2-d]pyrimidin-4-yl] '-amine

Combine (7-bromo-pyrido[3,2-i/]pyrimidin-4-yl)-4-tert-butyl-phenyl)-amine (36 mg, 0.1 mmol), 2-(trifluoromethyl)phenyl-boronic acid (29 mg, 0.15 mmol) in 1,2-dimethoxyethane (10 mL) and bubble nitrogen into the mixture for 10 minutes. Add tetrakis(triphenylphosphine)palladium(0) (12 mg, 0.01 mmol) and 2M Na₂CO₃ (1 mL) and heat at 80°C for 48 hours. Let the mixture cool to room temperature, dilute with water (10 mL), and extract twice with EtOAc (10 mL each). Dry (Na₂SO₄), evaporate, and purify on a preparative silica gel plate (2000 micron) eluting with 75% hexane-EtOAc to provide (4-tert- butyl-phenyl)-[7-(2-trifluoromethyl-phenyl)-pyrido[3,2-(f|pyrimidin-4-yl]-amine as a light yellow solid. Mass spec. 422.2.

D. (4-tgrt-Butyl-phenyl)-[6-(2-trifluoromethyl-phenyl)-phthalazin-l-yll-amine (cmpd 4)

1. 4-Bromo-2-dibromomethyl-benzonitrile

Reflux a mixture of 4-Bromo-2-methyl-benzonitτile (19.6 g, 0.1 mol) and bromine (39.0 g, 0.22 mol) in carbon tetrachloride (500 mL) using a 500 watt sunlamp for 16 hours. Let cool to room temperature, and filter off succinimide. Evaporate the product fully to provide 4-bromo-2- dibromomethyl-benzonitrile as a yellow powder.

2. 5-Bromo-3-hydroxy-2,3-dihydro-isoindol-l-one

Combine 4-bromo-2-dibromomethyl-benzonitrile (7.0 g, 19.8 mmol) and acetonitrile (150 mL). Drip in a mixture of silver nitrate (7.0 g, 41.2 mmol) in water (40 mL) and reflux the resulting translucent yellow liquid for 72 hours. Evaporate the mixture, and add IM NaOH (100 mL). Extract twice with EtOAc (100 mL each). Dry the solution (Na₂SO₄), evaporate, and purify by silica gel chromatography (80% hexanes- EtOAc) to obtain 600 mg of 4-bromo-2-formyl-benzonitrile and 1250 mg of 5-bromo-3- hydroxy-2,3-dihydro-isoindol-l-one as a white solid. 3. 6-Brorno-phthalazin-l-ol

Combine 5-bromo-3-hydroxy-2,3-dihydro-isoindol-l-one (1.0 g, 4.39 mmol) and hydrazine hydrate (10 mL) and allow the suspension to stir at room temperature for 16 hours. Collect 6-Bromo- phthalazin-1-ol as a white solid. 4. 6-Bromo-l-chloro-phthalazine

Heat a mixture of 6-Bromo-phthalazin-l-ol (300 mg, 1.33 mmol) in POCl₃ (10 mL) at 90°C for 2 hours. Evaporate the POCl₃, and add ice (100 g) followed by careful addition of saturated NaHCO₃. Extract with EtOAc, dry (Na₂SO₄), and evaporate to provide 4-chloro-7-(2-trifluoromethyl-phenyl)- quinoline as a white solid.

5. (6-Bromo-phthalazin-l-yl)-(4-tert-butyl-phenyl)-amine

Heat a mixture of 6-bromo-l-chloro-phthalazine (500 mg, 2.05 mmol) and 4-(tert-butyl)aniline

(611 mg, 4.10 mmol) in 2-propanol (10 mL) at reflux for 3 hours. Evaporate the mixture, add IM NaOH (10 mL), extract twice with EtOAc (10 mL each), dry (Na₂SO₄), and evaporate to provide the crude product. Purify by silica gel chromatography, eluting with dichloromethane followed by 95% CH₂C1₂- MeOH to provide (6-Bromo-phthalazin-l-yl)-(4-tert-butyl-phenyl)-amine as a white solid. 6. (4-tert-Butyl-phenyl)-[6-(2-trifluoromethyl-phenyl)-phthalazin-l-yl] -amine

Combine (6-bromo-phthalazin-l-yl)-(4-te7-t-butyl-phenyl)-amine (60 mg, 0.19 mmol), 2- (trifluoromethyl)phenyl-boronic acid (50 mg, 0.26 mmol) in 1,2-dimethoxyethane (10 mL) and bubble nitrogen into the mixture for 10 minutes. Add tetrakis(tτiphenylphosphine)palladium(0) (12 mg, 0.01 mmol) and 2M Na₂CO₃ (1 mL) and heat at 80 C for 48 hours. Let the mixture cool to room temperature, dilute with water (10 mL), and extract twice with EtOAc (10 mL each). Dry (Na₂SO₄), evaporate, and purify on a preparative silica gel plate (2000 micron) eluting with 75% hexane- EtOAc to provide (4- tert-butyl-phenyl)-[6-(2-trifluoromethyl-phenyl)-phthalazin-l-yl]-amine as a straw colored solid. Mass Spec. 421.2.

E. (4-tert-Butyl-phenyl)-[7-(2-trifluoromethyl-phenyl)-pyridor2.3-dlpyrimidin-4-yll-amine (cmpds 5 and 6}

1. Oxo-3-phenyl-propionaldehyde

Heat a mixture of toluene and sodium ethoxide (40 mL of a 21% ethanolic solution) to 50°C. Add 2-trifluoromethylacetophenone (20.0 g, 0.11 mol) and ethyl formate (11.8 g, 0.16 mol), and let stir at 65°C for 12 hours. Allow mixture to cool to room temperature and add 300 mL of diethyl ether. Collect the precipitate to obtain the sodium salt of 3-oxo-3-phenyl-propionaldehyde.

2. 7-(2-Trifluoromethyl-phenyl)-lH-pyrido[2,3-d]pyrimidine-2,4-dione

Finely divide the sodium salt of 3-oxo-3-phenyl-propionaldehyde (10.0 g, 0.043 mol) and add 50 mL of 90% phosphoric acid. Let stir until fully dissolved. Separately, similarly dissolve 6-amino- lH-pyrimidine-2,4-dione 5.7 g, 0.043 mol) in 50 mL of 90% phosphoric acid. Combine the 2 solutions and let stir for 12 hours at 100°C. Let the solution cool to room temperature, add 300 mL of water, and collect the product as a sticky solid. Triturate with ether to obtain 7-(2-trifluoromethyl-phenyl)-lH- pyrido[2,3-d]pyrimidine-2,4-dione as a white solid.

3. 2, 4-Dic ιloro- 7-(2-trifluoromethyl-phenyl)-pyrido[2, 3-d] pyrimidine

Heat a mixture of 7-(2-Trifluoromethyl-phenyl)-lH-pyrido[2,3-d]pyrimidine-2,4-dione (5.0 g,

0.016 mol) and POCl₃ (100 mL) at 90°C for 36 hours. Evaporate the POCl₃, and add ice (400 g) followed by careful addition of saturated NaHC0₃. Extract twice with EtOAc, dry (Na₂SO₄), and evaporate to provide 2,4-Dichloro-7-(2-trifluoromethyl-phenyl)-pyrido[2,3-d]pyrimidine.

4. (4-tert-Butyl-phenyl)-[2-clιloro-7-(2-trifluoromethyl-phenyl)-pyrido[2,3-d]pyrimidm-4-yl]- amine (cmpd 5)

To a mixture of diisopropylethylamine (260 mg, 2.0 mmol) in acetonitrile (5 mL), add t- butylaniline (124 mg, 1.0 mmol) followed by (4-tert-Butyl-phenyl)-[2-chloro-7-(2-trifluoromethyl- phenyl)-pyrido[2,3-d]pyrimidin-4-yl]-amine (310 mg, 1.0 mmol). Heat the mixture to 80°C for six hours. Evaporate the solvent, and partition between IM NaOH and EtOAc. Dry the solvent (Na₂SO₄) and evaporate. Purify by silica gel chromatography (1:1 hexanes/EtOAc to furnish the monoaniline (4- tert-Butyl-phenyl)-[2-chloro-7-(2-trifluoromethyl-phenyl)-pyrido[2,3 -d]pyrimidin-4-yl] -amine as a yellow solid.

5. (4-tert-Butyl-phenyl)-[7-(2-trifluoromethyl-phenyl)-pyrido[2,3-d]pyrimidin-4-yl] -amine (cmpd 6)

The 2-chloro substituent in (4-tert-Butyl-phenyl)-[2-chloro-7-(2-trifluoromethyl-phenyl)- pyrido[2,3-d]pyrimidin-4-yl]-amine can be removed using a number of reducing conditions known to those skilled in the art of organic synthesis e.g. hydrogenolysis or treatment with aluminum hydride reducing agents (See, e.g., Hudliclcy, M. Reductions in Organic Chemistry, ACS Monograph 188: 1996).

F. [7-(3-fluoro-pyridin-2-yl)-quinazolin-4-yll-(5-trifluoromethyl-pyridin-2-yl)-amine (cmpd 7)

1. 7-bromo-4-chloro-quinazoline

Reflux a solution of 7-bromo-3H-quinazolin-4-one (1.24 g, 0.0055 mol) in POCl₃ for

3.5h. Remove the excess POCl₃ under reduced pressure and partition the residue between EtOAc and saturated aqueous NaHCO₃. Dry the EtOAc layer and remove the solvent under reduced pressure to give 7-bromo-4-chloro-quinazoline as a yellow solid.

2. (7-bromo-quinazolin-4-yl)-(5-trifluoromethyl-pyridin-2-yl)-amine

Heat a mixture of 7-bromo-4-chloro-quinazoline (200 mg, 0.821 mmol) and 2-amino-5- trifluoromethyl-pyridine (239 mg, 1.48 mmol) at 230°C for 2 minutes. Cool and partition the solid residue between EtOAc and 10% NaOH. Dry the EtOAc layer (Na₂SO₄), remove the solvent under reduced pressure, and purify via flash chromatography to yield (7-bromo-quinazolin-4-yl)-(5- trifluoromethyl-pyridin-2-yl)-amine as a yellow solid.

3. 3-fluoro-2-tributylstannanyl-pyridine O .Sn(n-bu)₃ N

Cool a solution of 2-bromo-3-fluoro-pyridine (542 mg, 3.08 mmol) in dry THF to -78°C using a dry ice acetone bath. Add n-butyl-lithium (1.6 M in THF, 2.0 mL) to the reaction mixture dropwise via syringe over a 20 minute period. After stirring for 1.5 hours at -78°C, add tributyltin chloride slowly via syringe and remove the cooling bath. After 2 hours, partition the reaction mixture between EtOAc and brine, dry the EtOAc layer (Na₂S0₄) and remove the solvents under reduced pressure. Flash chromatography (ether/hexanes) yields 3-fluoro-2-tributylstannanyl-pyridine as a colorless oil.

4. [7-(3-fluoro-pyridin-2-yl)-quinazolin-4-yl]-(5-trifluoromethyl-pyridin-2-yl)-amine

Using procedures analogous to those given above, [7-(3-fluoro-pyridin-2-yl)-quinazolin-4-yl]-(5- trifluoromethyl-pyridin-2-yl)-amine is prepared by coupling (7-bromo-quinazolin-4-yl)-(5- trifluoromethyl-pyridin-2-yl)-amine to 3-fluoro-2-tributylstannanyl-pyridine. Mass spec. 385.1.

G. (4-tert-butyl-phenyl)-(7-pyridin-2-yl-quinazolin-4-yl)-amine (cmpd 8)

1. 4-bromo-2-nitro-benzonitrile

Stir the mixture of l,4-dibromo-2-nitro-benzene (3.56 mmol)and CuCN (3.74 mmol) in DMA (4 ml) at 100°C for 5hours. Cool to room temperature, dilute with EtOAc, filter through celite, wash the organic layer with brine, dry over Na₂SO₄, and concentrate under vacuum. Purify the residue by flash chromatography (4:1 hexanes/EtOAc) to give 4-bromo-2-nitro-benzonitrile.

2. 2-amino-4-bromo-benzonitrile

To a suspension of 4-bromo-2-nitro-benzonitrile (2.60 g, 0.0115 mol) in 12N HCl at 0°C, add SnCl₂-2H₂O portionwise. As the reaction is stirred vigorously, a white precipitate will form. After lh add ice to the reaction vessel followed by ION NaOH until the solution is basic. Extract the aqueous mixture with ether (2x) and EtOAc (lx) and wash the combined organic layers with brine. Dry the solution (Na₂SO₄) and remove the solvents under reduced pressure to give 2-amino-4-bromo-benzonitrile as a beige solid.

3. 7-bromo-3H-quinazolin-4-one

To a solution of 2-amino-4-bromo-benzonitrile (550 mg, 2.79 mmol) in formic acid, add sodium acetate (435 mg, 5.30 mmol) in one portion. Reflux the reaction mixture for 16h then remove the formic acid under reduced pressure to give a solid. Add 20% aqueous NaOH and stir for lhour. Remove the undissolved solids via filtration and acidify the filtrate with 12N HCl to produce a white solid. Collect the solid via filtration and wash it with water (5x) and ether (lx) to give 7-bromo-37J-quinazolin-4-one as an off-white solid.

4. 7-pyridin-2-yl-3H-quinazolin-4-one

To a solution of 7-bromo-3H-quinazolin-4-one (100 mg, 0.444 mmol) in toluene/dioxane (3:1), add 2-tributylstannanyl-pyridine (162 mg, 0.444 mmol) followed by tetτakis-(tτiphenylphosphine)- palladium(O) (26 mg, 0.022 mmol). Bubble dry nitrogen through the solution for 10 minutes then heat the stirring solution to 115°C under a nitrogen atmosphere. After several minutes the reaction mixture becomes homogeneous. After 16 hours, cool the reaction vessel and collect the precipitate via filtration. Wash the solid with 25% EtOAc/hexanes followed by hexanes to give 7-pyridin-2-yl-3H-quinazolin-4- one as a beige solid. 5. (4-tert-butyl-phenyl)-(7-pyridin-2-yl-quinazolin-4-yl)-amine

Using procedures analogous to those given above, (4-tert-butyl-phenyl)-(7-pyridin-2-yl- quinazolin-4-yl)-amine is prepared from 4-chloro-7-pyridin-2-yl-quinazoline and 4-tert-butylaniline. Mass spec. 354.2. H. (4-tgrt-Butyl-phenyl)- 7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yll-amine hydrochloride (cmpd 9)

1. 2-(4-bromo phenyl)-3-(trifluoromethyl)-pyridine

To a de-gassed mixture of 2-bromo-3-(trifluoromethyl)-pyridme (2.26 mmol), 4-bromo- phenylbronic acid (2.49 mmol), and 2M Na₂CO₃ (5.65 mmol), in DME (10 mL) under nitrogen add Pd(PPh₃)₄ (0.09 mmol). Stir the mixture at 80°C for overnight, concentrate, extract with EtOAc. Dry over Na₂SO , concentrate under vacuum, and purify by flash chromatography (4:1 hexanes/EtOAc) to give 2-(4-bromo phenyl)-3-(trifluoromethyl)-pyridine. 2. 2-(4-bromo-3-nitro-phenyl)-3-(trifluoromethyl)-pyridine

To a solution of 2-(4-bromophenyl)-3-(trifluoromethyl)-pyridine (0.93 mmol) in H₂SO₄ (4 mL) cautiously add fuming HNO₃ (2 ml). Stir the mixture 30 minutes at room temperature. Pour the mixture onto ice-water (20 mL) and collect the precipitate. Dissolve the precipitate in EtOAc and neutralize with saturated NaHCO₃, dry over a SO₄, concentrate under vacuum to obtain 2-(4-bromo-3-nitro-phenyl)-3-

(trifluoromethyl)-pyridine.

3. 2-nitro-4(3-trifluoromethyl-pyridin-2-yl)-benzonitrile

To a solution of 2-(4-bromo-3-nitro-phenyl)-3-(trifluoromethyl)-pyridine (0.55 mmol) in DMA (4 mL) add CuCN (0.60 mmol). Stir the mixture 4 hours at 110°C. Cool to room temperature, dilute with 20 ml of EtOAc, and filter through celite pad. Wash the filtrated with brine, dry over Na₂SO₄, concentrate under vacuum, and purify by flash chromatography (1:1 hexanes/EtOAc) to give 2-nitro-4(3- trifluoromethyl-pyridin-2-yl)-benzonitrile.

4. 2-amino-4-(3-trifluoromethyl-pyridin-2-yϊ)-benzo-nitrϊle

To an ice-water cooled solution of 2-ni1ro-4-(3-hifluoromethyl-pyridin-2-yl)-benzonitrile (0.44 mmol) in cone. HCl (6 mL) add SnCl₂ (1.457 mmol). Stir the mixture 2 h at room temperature. neutralize with NaOH, extract with EtOAc, dry over Na₂SO₄, and concentrate under vacuum. Purify the residue by flash chromatography (4:1 hexanes/EtOAc) to give 2-amino-4(3-trifluoromethyl-pyridin-2- yl)-benzo-nitrile.

5. 7-(3-trifluoromethyl-pyήdin-2-yl)-quinazolin-4-ol

Reflux 2-amino-4-(3-trifluoromethyl-pyridin-2-yl)-benzo-nitrile (0.41 mmol) and NaOAc (1.23 mmol) for 16 h in HCOOH (10 mL). Evaporate the solvent in vacuo, suspend the residue in 20 ml of 20% NaOH, stir for 30 min at room temperature. Filter, extract with EtOAc, dry over Na₂SO , and concentrate under vacuum to give 7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-ol. 6. 4-chloro- 7-(3-trifluoromethyl-pyridin-2-yl)-quinazoline

Reflux 7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-ol (0.38 mmol) for 18 hours in POCl₃ (5 mL). Evaporate the solvent in vacuo, then carefully neutralize with saturated NaHC0₃, and extract with EtOAc. Dry over Na₂SO₄, concentrate under vacuum to obtain 4-chloro-7-(3-trifluoromethyl-pyridin-2- yl)-quinazoline.

7. (4-tert-Butyl-phenyl)-[7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl] hydrochloride

Stir 4-chloro-7-(3-trifluoromethyl-pyridin-2-yl)-quinazoline (0.16 mmol) and 4-tert-butyl-aniline

(0.32 mmol) in EPA (4 mL) at 80°C for 6 hours. Cool the mixture and collect the precipitate to obtain (4- tert-Butyl-phenyl)-[7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl] hydrochloride. Mass spec. 422.2.

L (4-tert-Butyl-phenyl)-r2-methyl-7-(3-1rifluoromethyl-pyridin-2-yl)-quinazolm-4-yll-amine hydrochloride (cmpd 10)

1. 2-amino-4-(3-trifluoromethyl-pyridin-2-yl)-benzamide

11

Stir a mixture of 2-amino-4(3-trifluoromethyl-pyridin-2-yl)-benzo-nitrile (0.50 mmol) in 70% H₂SO₄ (10 ml) at 110°C for lhour. Cool to room temperature, neutralize with NaOH, extract with EtOAc, dry over Na₂SO₄, and concentrate under vacuum. Purify the residue by flash chromatography (3:2 hexanes/EtOAc) to give 2-amino-4-(3-trifluoromethyl-pyridin-2-yl)-benzamide.

2. 2-acetylamino-4-(3-trifluorornethyl-pyridin-2-yl)-benzamide

To a solution of 2-amino-4-(3-trifluoromethyl-pyridin-2-yl)-benzamide (0.5 mmol) and pyridine (0.55 mmol) in THF (5 ml) add acetyl chloride (0.55 mmol). Stir the mixture 10 minutes at room temperature. Concentrate under vacuum, extract with EtOAc, wash with brine, dry over Na₂SO₄, and concentrate under vacuum. Triturate with ether to give 2-acetylamino-4-(3-trifluoromethyl-pyridin-

2-yl)-benzamide.

3. 2-methyl- 7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-ol

Suspend 2-acetylamino-4-(3-trifluoromethyl-pyridin-2-yl)-benzamide in 20 ml of 20% NaOH, stir for 30 minutes at room temperature. Filter, acidify to pH=6, extract with EtOAc, and concentrate under vacuum to give 2-methyl-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-ol.

4. 4-chloro-2-methyl-7-(3-trifluoromethyl-pyridin-2-yl)-quinazoline

Using procedures analogous to those already described 4-chloro-2-methyl-7-(3-trifluoromethyl- pyridin-2-yl)-quinazoline is prepared from 2-methyl-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-ol. 5. (4-tert-Butyl-phenyl)-[2-methyl-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-amiiie

Using procedures analogous to those already described, (4-tgrt-Butyl-phenyl)-[2-methyl-7-(3- trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-amine is prepared by condensing 4-chloro-2-methyl-7-(3- trifluoromethyl-pyridin-2-yl)-quinazoline with 4-tgrt-butylaniline. Mass spec. 436.2.

J. r7-(3-Methyl-pyridin-2-yl)-quinazolin-4-yll-(4-tτifluoromethyl-phenyl)-amine (cmpd 11)

1. 7-[B(OH)J-3H-quinazolin-4-one

Reflux a mixture of 3-amino-4-carboethoxy-phenylboronic acid (1.46 g, 0.007 mol), prepared according to the procedure of Torssell et. al. (1957) Arkiv Kemi 10:491, and formamidine acetate (1.17 g,

0.008 mol) in methoxyethanol for 7 hours. Add an additional equivalent of formamidine acetate and continue to reflux for 16 hours. Cool the dark solution and remove the solvent under reduced pressure.

Add -100 mL of water, stir for 10 minutes, and collect the light gray solid on a sintered glass funnel.

Wash the solid with water (3x), dry, and recrystallize from methanol to give 7-[B(OH)₂]-3H-quinazohn- 4-one as a white solid.

2. 7-(3-Methyl-pyridin-2-yl)-3H-quinazolin-4-one

Purge a solution of 7-[B(OH)₂]-3i7-quinazolin-4-one (115 mg, 0.605 mmol), 2-bromo-3-methyl- pyridine (103 mg, 0.605 mmol), Na₂CO₃ (0.757 mL, 1.51 mmol, 2M aqueous solution), and DMF (4 mL) with nitrogen for 10 minutes. Add a catalytic amount of tetrakis-(triphenylphosphine)-palladium(0) (35 mg, 0.03 mmol) and heat at 95°C for 16 hours. Cool the reaction mixture, dilute with water and extract with ethyl acetate. Dry the combined organic layers (Na₂SO₄), concentrate under reduced pressure, and purify the crude product using silica gel chromatography (MeOH/CH₂Cl₂) to give 7-(3-Methyl-pyridin-2- yl)-3H-quinazolin-4-one . 3. [7-(3-Methyl-pyridin-2-yl)-quinazolin-4-yl]-(4-trifluoromethyl-phenyl)~amine

Using procedures analogous to those described above (see, for example, Schemes 1 and 2), [7- (3-Methyl-pyridin-2-yl)-quinazolin-4-yl]-(4-trifluoromethyl-phenyl)-amine is prepared from 7-(3- Methyl-pyridin-2-yl)-3H-quinazolin-4-one in two steps. Mass spec. 380.1.

K. (4-tert-Butyl-phenyl)-r7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yll-amine hydrochloride (cmpd 9)

1. 2-p-tolyl-3-trifluorornethyl-pyridine

To a de-gassed mixture of 2-chloro-3-(trifluoromethyl)-pyridine (70.1 mmol), -tolylboronic acid (70.6 mmol), and 2M Na₂CO₃ (175.0 mmol), in DME (200 mL) under nitrogen add Pd(PPh₃)₄ (2.8 mmol). Stir the mixture at 80°C for overnight, concentrate, extract with EtOAc. Dry over Na₂SO₄, concentrate under vacuum, pass a silica gel pad to give 2-/ tolyl-3-trifluoromethyl-pyridine. 2. 2-(4-methyl-3-nitro-phenyl)-3-(trifluoromethyl)-pyridine

To a solution of 2- )-tolyl-3-trifluoromethyl-pyridine (8.4 mmol) in H₂SO₄ (6 mL) cautiously add fuming HNO₃ (2 ml). Stir the mixture 60 minutes at room temperature. Pour the mixture onto ice-water

(30 mL), extract with EtOAc, neutralize with 1 N NaOH, dry over Na₂SO₄, and concentrate under vacuum to obtain 2-(4-methyl-3 -nitro-phenyl)-3 -(trifluoromethyl)-pyridine.

3. 2-nitro-4-(3-trifluoromethyl-pyridin-2-yl)-benzoic acid

To a solution of 2-(4-methyl-3-nitro-phenyl)-3-(trifluoromethyl)-pyridine (7.1 mmol) in the mixture of pyridme (10 mL) and water (5 ml) add KMnO₄ (25.3 mmol) portionwise. Stir the mixture 4 hours at 110°C then add another 25.3 mmol of KMnO₄ with 10 ml of water. Stir the mixture at 110°C for overnight. Cool to room temperature, filter through celite pad. Concentrate the filtrate under vacuum, dilute with water, and wash the aqueous with EtOAc. Neutralize the aqueous with 2 N HCl and collect the precipitate to give 2-nitro-4(3-trifluoromethyl-pyridin-2-yl)-benzoic acid.

4. 2-amino-4-(3-trifluoromethyl-pyridin-2-yl)-benzoic acid

Hydrogenate the solution of 2-nitro-4-(3-trifluoromethyl-pyridin-2-yl)-benzoic acid (3.84 mmol) in 95% EtOH (100 mL) with 10%Pd-C (150 mg) for over night. Filter through a celite pad and concentrate the filtrate to give 2-amino-4(3-trifluoromethyl-pyridin-2-yl)-benzoic acid.

5. 7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-ol

Stir the mixture of 2-amino-4-(3-trifluoromethyl-pyridin-2-yl)-benzoic acid (1.95 mmol) in

HCONH₂ (10 mL) for 4 hours at 145°C. Cool to room temperature, dilute with 20 ml of water, and collect the precipitate to give 7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-ol.

6. (4-tert-Butyl-phenyl)-[7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl] -amine hydrochloride

Using procedures analogous to those described above (see, for example, Schemes 1 and 2), (4- tert-Butyl-phenyl)-[7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-amide hydrochloride is prepared from 7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-ol in two steps. Mass spec. 422.2.

L [6-(propane-2-sulfonvU-pyridin-3-yll-r7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yll-amine hydrochloride (cmpd 12)

1. 2-Isopropylsulfanyl-5-nitro-pyridine

Stir the mixture of 2-mercapto-5-nitropyridine (10.0 mmol) and NaH (14.0 mmol) in DMA (10 ml) at room temperature for 30 minutes. Add 2-iodopropane (11.0 mmol) and stir overnight at room temperature. Dilute with H₂0, extract with EtOAc, wash with brine, dry over Na₂S0₄, and concentrate under vacuum. Purify the residue by flash chromatography (9:1 hexanes/EtOAc) to give 2-isopropyl- sulfanyl-5-nitro-pyridine.

2. 5-Nitro-2- (propane-2-sulfonyl)-pyridine

Heat the mixture of 2-isopropyl-sulfanyl-5-nitro-pyridine (3.5 mmol) and KMnO₄ (14.1 mmol) in HO Ac (15 ml) at 110°C for overnight. Filter, concentrate the filtrate, and neutralize with NaHCO₃. Extract with EtOAc, wash with brine, dry over Na₂SO₄, and concentrate under vacuum to give 2-(propyl-2-sulfonyl)-5-nitro-pyridine. 3. 6-(Propane-2-sulfonyl)-pyridin-3-ylamine

Suspend 2-(propyl-2-sulfonyl)-5-nitro-pyridine (0.44 mmol) in 10 ml of cone. HCl, add SnC12 dihydrate (1.43 mmol), and stir for 2 hours at room temperature. Neutralize with NaOH. Extract with EtOAc, wash with brine, dry over Na₂S0₄, and concentrate under vacuum to give 6-(propane-2- sulfonyl)-pyridin-3-ylamine.

4. [6-(propane-2-sulfonyl)-pyridin-3-yl]-[7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]- amine hydrochloride

Use the method described in Example 1 H.7 to obtain [6-(propane-2-sulfonyl)-pyridin-3-yl]-[7- (3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-amine hydrochloride. Mass spec. 473.1.

M. Additional Representative Substituted Ouinazolin-4-ylamine Analogues

Using routine modifications, the starting materials may be varied and additional steps employed to produce other compounds encompassed by the present invention. Compounds listed in Table II were prepared using the above methods, with readily apparent modifications. Table II Representative Substituted Ouinazoline-4-ylamine Analogues

Other compounds that were prepared are listed in Table III. Abbreviations used are: Ph=phenyl; Py=pyridin; Me=methyl; Et=ethyl; tBu=tert-butyl; thia=(l,3,4)thiadiazol; pyraz==lH-pyrazol; isopr=isopropyl; MeO=methoxy; EtO=ethoxy; PrO=propyloxy; MeA=methylamino; EtA=ethylamino; PrA=propylamino; BuA=butylamino; DMA=dimethylamino; DEA=diethylamino. Variable positions indicated in Table III are as shown on the following structure:

Table III

Other compounds that were prepared are listed in Table IN. Abbreviations used are as described above for Table III. Variable positions are as shown on the following structure:

Table IN

Additional compounds that were prepared are listed in Table N. Abbreviations used are as described above for Table III. Variable positions are as shown on the following structure:

Table V

The Ari -Matrix, Het-Matrix, and Ar2-Matrix tables below set forth a number of additional representative compounds that may be prepared by methods analogous to those shown above. Compounds can be formed by combining any element from the Het-Matrix with any elements from the Ari and Ar2 matrices. For example, the combination of element 111 from the Ari -Matrix, with element 202 from the Het-matrix, gives the moiety 111202. This moiety is then combined with element 312 from the Ar2-matrix, to form compound 111202312, which is (7-pyridin-2-yl-quinazolin-4-yl)-(4- trifluoromethyl-phenyl)-amine.

Ari -Matrix

Het-Matrix

Ar2-Matrix

EXAMPLE 2 Preparation of Representative Compounds This Example illustrates the preparation of representative substituted 2-aminoalkyl-quinazolin-4- ylamine analogues.

A. [2-Pyrrolidin-l -ylmethyl-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yll-(4-trifiuoro methyl- phenvD-amine (cmpd 308)

1. 2-p-tolyl-3-trifluoromethyl-pyridine

To a de-gassed mixture of 2-chloro-3-(trifluoromethyl)-pyridine (70.1 mmol), -tolylboronic acid (70.6 mmol), and 2M Na₂CO₃ (175.0 mmol), in dimethyl ether (DME; 200 mL) under nitrogen, add Pd(PPh₃)₄ (2.8 mmol). Stir the mixture at 80°C overnight, concentrate, and extract with EtOAc. Dry over Na₂SO₄, concentrate under vacuum, and pass through a silica gel pad to give 2-/>-tolyl-3- trifluoromethyl-pyridine . 2. 2-(4-methyl-3-nitro-phenyl)-3-(trifluoromethyl)-pyridine

To a solution of 2- >-tolyl-3-trifluoromethyl-pyridine (8.4 mmol) in H₂SO₄ (6 mL) cautiously add fuming HNO₃ (2 ml). Stir the mixture for 60 minutes at room temperature. Pour the mixture onto ice- water (30 mL), extract with EtOAc, neutralize with 1 N NaOH, dry over Na₂SO , and concentrate under vacuum to obtain 2-(4-methyl-3-nitro-phenyl)-3-(trifluoromethyl)-pyridine.

3. 2-nitro-4-(3-trifluoromethyl-pyridin-2-yl)-benzoic acid

To a solution of 2-(4-methyl-3-nitro-phenyl)-3-(trifluoromethyl)-pyridine (7.1 mmol) in a mixture of pyridine (10 mL) and water (5 ml) add KMnO₄ (25.3 mmol) portionwise. Stir the mixture for 4 hours at 110°C then add another 25.3 mmol of KMnO₄ with 10 ml of water. Stir the mixture at 110°C overnight. Cool to room temperature, and filter through celite pad. Concentrate the filtrate under vacuum, dilute with water, and wash the aqueous solution with EtOAc. Neutralize the aqueous solution with 2 N HCl and collect the precipitate to give 2-nitro-4(3-trifluoromethyl-pyridin-2-yl)-benzoic acid.

4. 2-nitro-4-(3-trifluoromethyl-pyridin-2-yl)-benzamide

Reflux a mixture of 2-amino-4(3-trifluoromethyl-pyridin-2-yl)-benzoic acid (25 g) with SOCl₂ (50 ml) for 4 hours and concentrate. Dissolve the residue in dichloromethane (DCM), cool with ice- water bath, pass NH₃ gas through the solution for 30 minutes, and stir for 15 minutes at room temperature. Concentrate and wash with water to give 2-nitro-4-(3-trifluoromethyl-pyridm-2-yl)- benzamide.

5. 2-amino-4-(3-trifluoromethyl-pyridin-2-yl)-benzamide

Hydrogenate 2-nitro-4-(3-frifluoromethyl-pyridin-2-yl)-benzamide (l.Og, 0.0032 mol) with 50 psi of H₂ and 100 mg of 10% Pd/C in ethanol. After 16 hours, filter the mixture through celite and concentrate under reduced pressure to give 2-amino-4-(3-trifluoromethyl-pyridin-2-yl)-benzamide as a solid.

6. 2-chloromethyl-7-(3-trifluoromethyl-pyridin-2-yl)-3H-quinazolin-4-one

Heat a solution of 2-amino-4-(3-trifluoromethyl-pyridin-2-yl)-benzamide (100 mg, 0.356 mmol) in 2-chloro- 1,1,1-trimethoxyethane (bp 138°C) at 130°C for 4 hours. Concentrate the mixture under reduced pressure to give 2-chloromethyl-7-(3-trifluoromethyl-pyridin-2-yl)-3H-quinazolin-4-one as an oil which crystallizes on standing. 7. 4-chloro-2-chloromethyl- 7-(3-trifluoroιnethyl-pyridin-2-yl)-quinazoline

Reflux a mixture of 2-chloromethyl-7-(3-trifluoromethyl-pyridin-2-yl)-3/J-quinazolin-4-one

(obtained from the reaction above) and POCl₃ for 16 hours. Cool the mixture and concentrate under reduced pressure. Partition the residue between EtOAc and saturated NaHCO₃ solution. Wash the EtOAc portion with additional NaHCO₃ and then dry (Na₂SO ) and concentrate under reduced pressure.

Filter the brown residue through 2 inches of silica gel (1:1 EtOAc/hexanes eluent) and concentrate under reduced pressure to give 4-chloro-2-chloromethyl-7-(3-trifluoromethyl-pyridin-2-yl)-quinazoline. 8. [2-chloromethyl- 7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(4-trifluoro methyl- phenyl)-amine

Heat a mixture of 4-chloro-2-chloromethyl-7-(3-trifluoromethyl-pyridin-2-yl)-quinazoline (42 mg, 0.117 mmol) and 4-trifluoromethyl-aniline (19 mg, 0.117 mmol) in isopropyl alcohol (1 mL) at 75°C for 4 hours. Cool the mixture and wash the precipitate with isopropyl alcohol followed by ether to give

[2-chloromethyl-7-(3-tiifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(4-trifluoromethyl-phenyl)-amine as the mono-HCl salt.

9. [2-Pyrrolidin-l-ylmethyl-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(4- tήfluoromethyl-phenyl)-amine

Heat a solution of [2-chloromethyl-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(4- trifluoromethyl-phenyl)-amine HCl (30 mg, 0.058 mmol) in pyrrolidine (lmL) at 100°C for 1 hour.

Remove the excess pyrrolidine under reduced pressure and partition the residue between EtOAc and 10% NaOH solution. Dry the EtOAc layer (Na₂SO₄) and concentrate under reduced pressure to give [2-

Pynolidin-l-ylmethyl-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(4-trifluoromethyl-phenyl)- amine as a foam. Mass Spec 517.2.

B. [2-(2.6-Dimethyl-moι holin-4-ylmethyl)-7-(2-trifluoromethyl-phenyl)-pyrido[4 3-d1pyrimidin-4-yll- (4-trifluoromemyl-phenyl)-amine (cis) (cmpd 309)

1. 4-Hydroxy-6-(2-trifluoromethyl-phenyl)-nicotinic acid ethyl ester

Dissolve Lithium bis(trimethylsilyl)amide (LiHMDS) (34 g, 0.20 mol) in dry THF (150 mL) and cool to -70°C under N₂ atm. Add 4-dimethylamino-3-ethoxy-but-3-en-2-one (15 g, 0.081 mol; see J. Heterocyclic Chem. (1987) 24:1669) and 2-(trifluoromethyl)benzoyl chloride (20.0 g, 0.097 mol) in THF (50 mL) into the solution for 10 minutes. Remove the cooling bath and stir for 10 minutes. Add ammonium acetate (10 g) and acetic acid (200 mL) to the reaction mixture and distil THF under reduced pressure. Heat the mixture at 60-65°C for 18 hours, cool and add water (250 mL) and CH₂C1₂ (250 mL). Separate the CH₂C1₂ layer, and extract the aqueous layer twice with CH₂C1₂ (2 x 250 mL each). Combine the CH₂C1₂ extracts, dry (MgSO ), and evaporate. Purify by silica gel chromatography to provide 4- Hydroxy-6-(2-trifluoromethyl-phenyl)-nicotinic acid ethyl ester as a yellow solid. 2. 4-Chloro-6-(2-trifluoromethyl-phenyl)-nicotinic acid ethyl ester

Heat a mixture of 4-Hydroxy-6-(2-trifluoromethyl-phenyl)-nicotinic acid ethyl ester (9.0 g, 0.029 mol) in POCl₃ (22 g) at 110°C for 2 hours. Evaporate the POCl₃, and add ice (100 g) followed by careful addition of saturated NaHCO₃. Extract with EtOAc, dry (MgSO₄), and evaporate to provide 4-chloro-6- (2-trifluoromethyl-phenyl)-nicotinic acid ethyl ester as a brown oil.

3. 4-Amino-6-(2-trifluoromethyl-phenyl)-nicotinamide

Heat a mixture of 4-ChloiO-6-(2-trifluoromethyl-phenyl)-nicotinic acid ethyl ester (5.2 g) and 28 % aq. NH₄OH (100 rnL) in a 350 ml resealable pressure vessel for 60 hours. Cool, extract with EtOAc (3 x 100 mL each), dry (MgSO₄), and evaporate to provide the crude product. Purify by silica gel cliromatography to provide 4-amino-6-(2-trifluoromethyl-phenyl)-nicotinamide as a solid.

4. 2-(2,6-Dimethyl-morphoUn-4-ylmethyl)-7-(2-trifluoroιnethyl-phenyl)-pyrido[4,3-d]pyήmidin- 4-ol

Heat a solution of 4-amino-6-(2-trifluoromethyl-phenyl)-nicotinamide (1 g, 3.5 mmol), 2,6- dimethyl-morpholin-4-yl)-acetic acid ethyl ester (2.85 g, 14 mmol), NaOEt (5.0 eq.) in EtOH (10 mL) for 20 hours. After cooling, concentrate the reaction mixture under reduced pressure, dilute the mixture with water (25 mL) and extract with EtOAc (3 x 25 mL each), then wash twice with water (25 mL each) and dry with MgSO . Evaporate, and purify by flash chromatography to obtain 2-(2,6-dimethyl-morpholin- 4-ylmethyl)-7-(2-trifluoromethyl-phenyl)-pyrido[4,3-d]- pyrimidin-4-ol.

5. 4-Chloro-2-(2,6-dimethyl-morpholin-4-ylmethyl)-7-(2-trifluoromethyl-phenyl)-pyrido[4,3- dj pyrimidine

Reflux a mixture of 2-(2,6-dimethyl-morpholin-4-ylmethyl)-7-(2-trifluoromethyl-phenyl)- pyrido[4,3-d]- pyrimidin-4-ol (0.6 g), 2,6-lutidine (0.62 g), and POCl₃ (1.1 g) in CHC1₃ (15 mL) for 20 hours. Cool the mixture and concentrate under reduced pressure. Partition the residue between EtOAc and saturated NaHCO_. solution. Wash the EtOAc portion with additional NaHCO₃ and then dry (N ₂SO₄) and concentrate under reduced pressure. Filter the brown residue through 2 inches of silica gel (1:1 EtOAc/hexanes eluent) and concentrate under reduced pressure to give 4-chloro-2-(2,6-dimethyl- morpholm-4-ylmethyl)-7-(2-trifluoromethyl-phenyl)-pyrido[4,3-d]pyrimidine. 6. [2-(2, 6-Dimethyl-morpholin-4-ylmethyl)- 7-(2-trifluoromethyl-phenyl)-pyrido[4, 3- d] pyrimidin-4-yl] -(4-trifluoromethyl-phenyl)-amine (cis)

Heat a mixture of 4-chloro-2-(2,6-dimethyl-morplιolin-4-ylmethyl)-7-(2-trifluoromethyl- phenyl)-pyrido[4,3-d]pyrimidine (43.7 mg, 0.1 mmol) and 4-trifluoromethyl-aniline (16.1 mg, O.lmmol) in AcCN (1 mL) at 80°C for 24 hours. Cool the mixture and wash the precipitate with ether to give 4- chloro-2-(2,6-dimethyl-morpholin-4-ylmethyl)-7-(2-trifluoiOmethyl-phenyl)-pyrido[4,3-d]pyrimidine as the mono-HCl salt. Mass Spec 561.2.

C. [2-Morpholin-4-ylmethyl-7(3 -trifluoromethyl-pyridin-2-yl)-pyrido [3.2-a]pyrimidin-4-yll -(4- trifluoromethyl-phenyD-amine (cmpd 310)

1. 6 '-Methoxy-3-trifluoromethyl-[2, 3 'Jbipyridinyl

Heat a mixture of 2-chloro-3-trifluoromethylpyridine (37 g, 0.2 mol), 2-methoxypyridine-5- boronic acid (32 g, 0.21 mol), tetrakis(triphenylphosphine) palladium(O) (9 g, 7 mmol) and 2M potassium carbonate (150 mL) in toluene (500 mL) under a nitrogen atmosphere, at 90°C for 8 hours. Cool the reaction mixture and separate the layers. Extract the aqueous layer with ethyl acetate (2 x 250 mL) and wash the combined organics with 4M sodium hydroxide (250 mL), water (250 mL), and brine (250 mL). Dry (MgSO₄) and concentrate under reduced pressure. Purify the oil by flash chromatography on silica gel (50% ether/50% hexane) to give the title compound (48.2 g, 95%) as a colorless oil. 2. 3-Trifluoromethyl-rH-[2,3]' bipyridinyl-6'-one

Heat 6'-methoxy-3-trifluoromethyl-[2,3']bipyridinyl (41 g, 0.16 mol) in 30% HBr/AcOH (100 mL) to reflux for 1 hour. Cool the mixture and filter, and wash the precipitate with ether (100 mL).

Transfer the precipitate into 10M sodium hydroxide (500 mL) and stir for 1 hour, and treat the solution with hydrochloric acid until the solution is pH 7. Collect the white solid by filtration and air dry to give the title compound (36 g, 93%) as a white solid.

3. 5'-Nitro-3-trifluoromethyl-l 'H-[2,3]' bipyridinyl-6'-one

To a solution of 3-trifluoromethyl-l'H-[2,3']bipyridinyl-6'-one (25 g, 0.1 mol) in concentrated sulfuric acid (100 mL) at 0°C, add dropwise a solution of fuming nitric acid (35 mL) and concentrated sulfuric acid (10 mL). Heat the reaction mixture to 70°C for 1 hour, cool and pour onto ice (500 mL).

Filter the mixture and treat the filtrate with 10 M sodium hydroxide until the solution is at pH 4-5.

Collect the precipitate by filtration and air dry to give the title compound (26.2 g, 92%) as a white solid.

I l l 4. 6'-Chloro-5 '-nitro-3-trifluoromethyl-[2, 3J ' bipyridinyl

Heat a solution of 5'-nitro-3-trifluoromethyl-l'H-[2,3']bipyridinyl-6'-one (25 g, 0.088 mol), thionyl chloride (300 mL) and DMF (3 mL) to reflux for 4 hours. Remove the volatiles by rotary evaporation and partition the residue between ethyl acetate (350 mL) and saturated sodium bicarbonate solution (250 mL). Extract the aqueous layer with further ethyl acetate (250 mL) and wash the combined organics with brine (250 mL). Dry (MgSO₄) and concentrate under reduced pressure to give the title compound (25 g, 93%) as a yellow oil.

5. 6'-Chloro-3-trifluoromethyl-[2,3]' bipyridinyl-5 '-ylamine

To a solution of 6'-chloro-5'-nitro-3-trifluoromethyl-[2,3']bipyridinyl (25 g, 0.082 mol) and calcium chloride (llg, 0.1 mol) in ethanol (300 mL) and water (50 mL), add iron powder (45 g, 0.82 mol). Heat the solution to reflux for 1.5 hours, cool and filter through Celite. Concentrate the mixture under reduced pressure, re-dissolve in ethyl acetate (300 mL) and wash with brine (200 mL). Concentrate the solution under reduced pressure and purify by flash chromatography on silica gel (50% ether/ 50% hexane) to give the title compound (19 g, 85%) as a pale yellow solid.

6. 3-Amino-5-[3-(trifluoromethyl)(2-pyridyl)]pyridine-2-carboxamide

Heat a solution of 6'-chloro-3-trifluoromethyl-[2,3']bipyridinyl-5 '-ylamine (25 g, 0.091 mol), zinc cyanide (6.75 g, 0.058 mol), tris[dibenzylidineacetone]di-palladium (also referred to as "pd₂(dba)₃";2.63 g, 2.86 mmol), l,r-bis(diphenylphosphino)ferrocene (also referred to as "DPPF"; 3.16g, 5.72 mmol) in DMF (250 mL) and water (2.5 mL), under a nitrogen atmosphere, at 120°C for 1 hour. Add water (30 mL) and heat the solution at 120°C for a further 4 hours to complete the hydrolysis. Cool the reaction to 0°C and add a solution of saturated ammonium chloride (200 ml), water (200 L) and concentrated ammonium hydroxide (50 mL). After stirring at 0°C for 1 hour, filter the yellow precipitate, and wash with water (200 mL) and a 1 : 1 mixture of ether-hexane (200 mL). Dry the solid in air and then in a vacuum oven to give (23 g, 90%_>) ofthe title compound. 7. 2-(Chloromethyl)-7-[3-(trifluoromethyl)(2-pyridyl)]-3-hydropyridino[3,2-d]pyrimidin-4-one

Heat a solution of 3-amino-5-[3-(trifluoromethyl)(2-pyridyl)]pyridine-2-carboxamide (23 g, 81.5 mmol) and 2-chloro- 1,1,1-trimethoxyethane (250 mL) at 130°C for 1 hour. Remove the volatiles by evaporation and triturate the solid (50% ether/ 50% hexane) to give the title compound as a light brown solid (21 g, 76%).

8. 2-(Morpholin-4-ylmethyl)- 7-[3-(trifluoromethyl) (2-pyridyl)]-3-hydropyήdino[3, 2- dJpyrimidin-4-one

Heat a solution of 2-(chloromethyl)-7-[3-(trifluoromethyl)(2-pyridyl)]-3-hydropyridino[3,2- d]pyrimidin-4-one (20 g, 0.058 mol), morpholine (15.66 g, 0.18 mol) in acetonitrile (500 mL) at 80°C for 12 hours. Evaporate the solution and partition the residue between ethyl acetate (500 mL) and saturated sodium bicarbonate solution (500 mL). Extract the aqueous layer with further ethyl acetate (250 mL) and wash the combined organics with brine (500 mL). Dry (MgSO₄) and concentrate under reduced pressure to give the title compound (18.8 g, 83%) as a brown solid.

9. 4-({4-Chloro-7-[3-(trifluoromethyl)(2-pyridyl)]pyridino[3,2-d]pyrimidin-2-yl}methyl)- methylmorpholine

Heat a solution of 2-(morpholin-4-ylmethyl)-7-[3-(trifluoromethyl)(2-pyridyl)]-3- hydropyridino[3,2-d]pyrimidin-4-one (11.73 g, 0.03 mol), POCl_. (13.8 g, 0.09 mol) and 2,6-lutidine (9.63 g, 0.09 mol) in chloroform (500 mL) at 60°C for 12 hours. Evaporate the solution and partition the residue between ethyl acetate (500 mL) and saturated sodium bicarbonate solution (500 mL). Extract the aqueous layer with further ethyl acetate (250 mL) and wash the combined organics with brine (500 mL). Dry (MgSO₄) and concentrate under reduced pressure to give the title compound (11.5 g, 94%) as a brown solid. 10. [2-Morpholin-4-ylmethyl- 7(3-trifluoromethyl-pyridin-2-yl)-pyrido[3, 2-a]pyrimidin-4-ylJ- (4-trifluoromethyl-phenyl)-amine

Heat a solution of 4-({4-chloro-7-[3-(trifluoromethyl)(2-pyridyl)]pyridino[3,2-d]pyrimidin-2- yl}methyl)-methylmorpholine (12.2 g, 0.03 mol), 4-(trifluoromethyl)aniline (4.8 g, 0.03 mol) in acetonitrile (500 mL) at 80°C for 12 hours. Evaporate the solution and partition the residue between ethyl acetate (500 mL) and saturated sodium bicarbonate solution (500 mL). Extract the aqueous layer with further ethyl acetate (2 x 250 mL) and wash the combined organics with brine (500 mL). Dry (MgSO₄) and concentrate under reduced pressure. Purify the residue by flash chromatography on silica gel (90% ether/ 10% hexane then 100% ether) to give the title compound (12.5 g, 78%). Mass Spec. 534.2.

P. [2-(2-Pvnolidin-l-yl-ethyl)-7-(3-tiifluoromethyl-pyridinv-2-yl)-quinazolin-4-yll-(4-trifluoromethyl- phenvD-amine (cmpd 311) 1. 3-Benzyloxy-propionic acid

In small portions, add sodium hydride (2.22 g, 60% dispersion in mineral oil, 55.4 mmol) to a cold (0°C) solution of benzyl alcohol (4.0 g, 37 mmol) in toluene (100 mL). Add ethyl 3- bromopropionate (8.0 g, 44 mmol) dropwise to the mixture, allow the resulting solution to warm to room temperature and stir for 1 hour. Quench the reaction with the addition of water until all bubbling ceases. Dilute the mixture with ethyl acetate (100 mL) and extract with water (100 mL) and brine (100 mL). Dry the organic extract over Na₂SO₄ and remove the solvent under reduced pressure to yield the crude ester as a clear oil. Dissolve the oil in methanol (20 mL) and 6 N NaOH (20 mL), and stir for 1 hour. Concentrate the mixture (approximately 20 mL) and dilute with water (20 mL). Extract the aqueous mixture once with CH₂C1₂ (40 mL). Acidify the aqueous phase with cone. HCl and extract with EtOAc (3 x 50 mL). Dry the combined EtOAc extracts over Na₂SO . Remove the solvent under reduced pressure to yield the title compound as a clear oil (2.28 g, 34.0 %) that solidifies upon standing.

2. 2-(2-Benzyloxy-ethyl)-7-(3-tήfluoromethyl-pyridiny-2-yl)-3H-quinazolin-4-one

Cool a solution of 3-benzyloxy-propionic acid (1.66 g, 9.19 mmol) in hexanes (40 mL) to 0°C and add oxalyl chloride (3.50 g, 27.6 mmol) dropwise. After the addition is completed, add DMF (2 drops), and stir the resulting mixture for 1 hour. Remove the solvent under reduced pressure and dissolve the crude acid chloride in dry THF (20 mL). In a separate flask, dissolve 2-amino-4-(3- trifluoromethyl-pyridin-2-yl)-benzamide (2.35 g, 8.37 mmol) in dry THF (40 mL) and pyridine (0.727 g, 9.19 mmol) and cool to 0°C. Add the solution containing the crude acid chloride dropwise to the second solution. Allow the mixture to warm to room temperature and stir for 1 hour. Add a solution of 10% NaOH_(aq) (20 mL) to the mixture and stir the solution for 1 hour. Concentrate the mixture (~20 mL), dilute with water (20 mL), and acidify with cone. HCl. Extract the resulting solution with EtOAC (3 x 50 mL). Wash the combined organic extracts with brine and dry over Na₂S0₄. Remove the solvent under reduced pressure to yield the title compound as a white solid (3.24 g, 82.9 %).

3. 2-(2-Benzyloxy-ethyl)4-chloro- 7-(3-trifluoromethyl-pyridiny-2-yl)-quinazoline

Dissolve 2-(2-benzyloxy-ethyl)-7-(3-trifluoromethyl-pyridiny-2-yl)-3H-quinazolin-4-one (3.24 g, 7.62 mmol) in CHC1₃ (40 mL) and 2,6-lutidine (2.45 g, 22.9 mmol). Add phosphorous oxychloride (1.77 mL, 19.0 mmol) dropwise and heat the resulting solution to reflux for 18 hours. Cool the solution and remove the solvent under reduced pressure. Partition the crude residue between EtOAc (200 mL) and saturated NaHCO₃ (aq) (200 mL). Remove the organic phase and extract the aqueous phase with EtOAc (200 mL). Combine the two organic extracts, wash with brine (200 mL), and dry over Na₂SO₄. Remove the solvent to yield the title compound as a light brown solid (2.47 g, 73.1 %).

4. [2-(2-Benzyloxy-ethyl)-7-(3-trifluoromethyl-pyridiny-2-yl)-quinazolin-4-yl]-(4- trifluoromethyl-phenyl)-atnine

Dissolve 2-(2-benzyloxy-ethyl)-4-chloro-7-(3 -trifluoromethyl-pyridiny-2-yl)-quinazoline (2.47 g, 5.57 mmol) into a solution of acetonitrile (50 mL) and 4-trifluoromethyl-aniline (0.986 g, 6.12 mmol). Heat the mixture to 80°C for 2 hours, to form a white precipitate. Cool the solution in an ice bath and add diethyl ether (25 mL). Filter off the white precipitate and dry in a vacuum oven to yield the title compound as the mono-hydrochloride salt (2.96 g, 87.8 %).

5. 2-[4-(4-Trifluoromethyl-phenylamino)-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-2-yl]- ethanol

Dissolve [2-(2-benzyloxy-ethyl)-7-(3-trifluoromethyl-pyridiny-2-yl)-quinazolin-4-yl]-(4- xrifluoromethyl-phenyl)-amine hydrochloride (2.96 g, 4.89 mmol) in MeOH (150 mL) and add 10% Pd/C

(200 mg). Hydrogenate the mixture at 50 p.s.i. at 60°C for 8 hours. Quickly filter the mixture through

Celite and wash the Celite filter cake with hot MeOH (200 mL). Remove the solvent under reduced pressure to yield the mono-hydrochloride salt of title compound as a white solid (1.75 g, 69.5 %).

6. [2-(2-Chloro-ethyl)-7-(3-trifluoromethyl-pyridiny-2-yl)-quinazolin-4-yl]-(4-trifluoromethyl- phenyl)-amine

Dissolve 2-[4-(4-hifluoromethyl-phenylaιmno)-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-2- yl]-ethanol hydrochloride (1.54 g, 2.99 mmol) in thionyl chloride (20 mL) and heat to 60°C for 1 hour. Remove the excess thionyl chloride under reduced pressure and triturate the residue with diethyl ether to yield the mono-hydrochloride salt ofthe title compound as a light brown solid (1.48 g, 92.8 %). 7. [2-(2-Pyrrolidin-l-yl-ethyl)-7-(3-trifluoromethyl-pyridiny-2-yl)-quinazolin-4-yl]-(4- trifluoromethyl-phenyl)-amine

Dissolve [2-(2-chloro-ethyl)-7-(3-trifluoromethyl-pyridiny-2-yl)-quinazolin-4-yl]-(4- trifluoromethyl-phenyl)-amine hydrochloride (20 mg, 0.0375 mmol) in CH₃CN / 10% diisopropylethylamine (0.187 mL) and add a 0.2 N solution of pyrrolidine in acetonitrile (0.281 mL). Heat the mixture at 70°C for 18 hours. Remove the solvent under reduced pressure and partition the crude reaction mixture between EtOAc (1 mL) and 1 N (NaOH). Remove the organic extract and extract the aqueous phase again with EtOAc (1 mL). Chromatograph the combined organic extracts through a small pad of silica gel, eluting with acetone to yield the title compound as a light brown solid (18 mg, 90 %).

E. [2-(3-moφholin-4-yl-propyl)7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(4- trifluoromethyl-phenvD-amine hydrochloride (cmpd 313) 1. 3-[4-hydroxy- 7-(3-triflιιoromethyl-pyridin-2-yl)-quinazolin-2-yl] -propionic acid ethyl ester

To a solution of 2-amino-4-(3-trifluoromethyl-pyridin-2-yl)-benzamide (0.5 mmol) and pyridine

(0.55 mmol) in THF (5 ml), add 3-chlorocarbonyl-propionic acid ethyl ester chloride (0.55 mmol). Stir the mixture for 20 minutes at room temperature, add 20 ml of 21% NaOEt in EtOH, and stir for 30 minutes at 50°C. Concentrate, add water, filter, acidify to pH 6, and collect the precipitate to give 3-[4- hydroxy-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-2-yl] -propionic acid ethyl ester.

2. 3-[4-chloro-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-2-yl] -propionic acid ethyl ester

Using procedures analogous to those already described, 3-[4-chloro-7-(3-trifluoromethyl- pyridin-2-yl)-quinazolin-2-yl]-propionic acid ethyl ester is prepared from 3-[4-hydroxy-7-(3- trifluoromethyl-pyridin-2-yl)-quinazolin-2-yl] -propionic acid ethyl ester.

3. 3-[4-(4-trifluoromethyl-phenylamino)-7-(3-trifluorornethyl-pyridin-2-yl)-quinazolin-2-yl]- propionic acid ethyl ester

Using procedures analogous to those already described, 3-[4-(4-trifluoromethyl-phenylamino)-7- (3-trifluoromethyl-pyridin-2-yl)-quinazolin-2-yl]-propionic acid ethyl ester is prepared from 3-[4-chloro- 7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-2-yl]-propionic acid ethyl ester. 4. 3-[4-(4-trifluoromethyl-phenylarnino)-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-2-yl]- propionic acid

To a mixture of 3-[4-(4-tτifluoromethyl-phenylamino)-7-(3-trifluoro-methyl-pyridin-2-yl)- quinazolin-2-yl] -propionic acid ethyl ester (0.5 mmol) in THF (20 ml) and H₂O (20 ml), add LiOH (1.5 mmol). Stir the mixture for 2 hours at 60°C. Concentrate, add water, extract with ether, acidify the aqueous layer to pH 4-5, extract with EtOAc, and concentrate to give 3-[4-(4-trifluoromethyl- phenylamino)-7-(3-trifluoro-methyl-pyridin-2-yl)-quinazolin-2-yl]-propionic acid.

5. l-morpholin-4-yl-3-[4-(4-trifluoromethyl-phenylamino)-7-(3-trifluoromethyl-pyridin-2-yl)- quinazolin-2-ylJ-propan-l-one (cmpd 312)

To a solution of 3-[4-(4-trifluoromethyl-phenylamino)-7-(3-trifluoro-methyl-pyridin-2-yl)- qumazolm-2-yl]-propionic acid (0.5 mmol) and triethylamine (0.5 mmol) in DMF (10 ml), add benzotriazol-l-yl-oxy-tris(dimethylamino)phosphonium hexafluorophosphate (BOP; 0.5 mmol). Stir the mixture for 18 hours at room temperature, dilute with water, extract with EtOAc, and wash with brine. Concentrate to give l-morpholin-4-yl-3-[4-(4-trifluoromethyl-phenylamino)-7-(3 -trifluoromethyl- pyridin-2-yl)-quinazolin-2-yl]-propan-l-one. Mass Spec. 575.2.

6. [2-(3-morpholin-4-yl-propyl)-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(4- trifluoromethyl-phenyl)-amine hydrochloride (cmpd 313)

To a solution of l-moφholin-4-yl-3-[4-(4-trifluoromethyl-phenylamino)-7-(3-trifluoromethyl- pyridin-2-yl)-quinazolin-2-yl]-propan-l-one (0.14 mmol) in THF (20 ml), add LAH (0.67 mmol). Stir the mixture for 6 hours at room temperature, quench with 10% NaOH, extract with EtOAc, dry over Na₂SO , and add HCl-EtOAc. Collect the precipitate to give £2-(3-morpholin-4-yl-propyl)-7-(3- trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(4-trifluoromethyl-phenyl)-amine hydrochloride. Mass Spec. 561.2. F. 4-trifluoromethylphenyl-[2-(2,6-dimethylmoφhonli-4-ylmethyl)-7 — (2 -trifluoromethyl phenyl)- quinazolin-4-yl] -amine (cmpd 314)

1. 7-Bromo-2-chloromethyl-3H-quinazolin-4-one

Reflux a solution of 2-amino-4-bromobenzamide (27 g, 0.13 mol; see Joshi and Chaudhari, (1987) Indian J. Chem., Sect. B, 26B(6):602-4) in 2-chloro- 1,1,1-trimethoxyethane (50 mL) for 30 minutes, during which time a large precipitate appears. Evaporate the mixture fully and triturate with ether to collect 28 g of 7-bromo-2-chloromethyl-3i7-quinazolin-4-one as a white solid.

2. 7-Bromo-4-chloro-2-chloromethylquinazoline

Heat a mixture of 7-bromo-2-chloromethyl-5H-quinazolin-4-one (5 g, 18.2 mmol), 2,6-lutidine (5 g), and phosphorus oxychloride (5 mL) in 1,2-dimethoxyethane (500 mL) at 80°C for 16 hours. Cool the mixture to room temperature and fully evaporate the mixture, then dilute with ether and wash with water. Dry the solvent (Na₂SO₄) and evaporate the ether to obtain 7-bromo-4-chloro-2- chloromethylquinazoline (3.5 g) as a yellow solid.

3. 7-Bromo-2-chlorometlιylquinazolin-4-yl)-(4-trifluoromethylphenyl)-aιnine

Heat a mixture of 7-bromo-4-chloro-2-chloromethylquinazoline (1168 mg, 4.0 mmol) and 4-

(trifluoromethyl)aniline (644 mg, 4.0 mmol) in chloroform (50 mL) at 60°C for 16 hours. Cool and collect the precipitated product 7-bromo-2-chloromethylquinazolin-4-yl)-(4-trifluoromethylphenyl)- amine (1021 mg) as the HCL salt.

4. [7-Bromo-2-(cis-2,6-dimethylmorpholin-4-ylmethyl)-quinazolin-4-yl]-4-(trifluoro methylphenyl)-amine

Heat a mixture of 7-bromo-2-chloromethylquinazolin-4-yl)-(4-trifluoromethylphenyl)-amine (416 mg, 1.0 mmol), cώ-2,6-dimethylmbφholine (150 mg, 1.3 mmol), and triethylamine (202 mg, 2.0 mmol) in N,N-dimethylacetamide (7 mL) for 1 hour. Cool to room temperature, dilute with EtOAc (50 mL), and wash four times with water (25 mL each). Dry (Na₂SO₄) and evaporate. Triturate with ether to give [7-bromo-2-(cz_^,-2,6-dimethylmoφholin-4-ylmethyl)-qumazolin-4-yl]-4-(trifluoromethylphenyl)- amine (430 mg) as a yellow solid.

5. [2-(cis-2, 6-dimethylmorpholin-4-yloxymethyl)- 7-(2-trifluoromethylphenyl)-quinazolin-4-yl]- (4-trifluoromethylphenyl)-amine

Under nitrogen, heat a mixture of [7-bromo-2-(cis-2,6-dimethylmoφholin-4-ylmethyl)- quinazolin-4-yl]-4-(trifluoromethylphenyl)-amine (75 mg, 0.15 mmol), 2-(trifluoromethyl phenyl)boronic acid (45 mg, 0.23 mmol), tetrakis(triphenylphosphine)palladium(0) (21 mg, 0.018 mmol), 2M Na₂CO₃ in water (lmL), and 1,2-dimethoxyethane (5 mL) at 60°C for 16 hours. Cool the mixture to room temperature, dilute with EtOAc, and wash twice with water (10 mL each). Dry the organic layer (Na₂SO₄) and evaporate. Purify by preparative TLC (9:1 CH₂CL₂:MeOH) to obtain [2-(cis- 2,6-dimethylmoφholin-4-yloxymethyl)-7-(2-τrifluoro methylphenyl)-quinazolin-4-yl]-(4- trifluoromethylphenyl)-amine (112 mg) as a yellow solid. Mass Spec. 560.2.

G. [7-(3-Methyl-pyridin-2-yl)-2-pyrrolidin-l-ylmethyl-pyridor3,2-d]pyrimidin-4-yll-(4-tτifluoromethyl- phenvD-amine (cmpd 315)

1. 5-Bromo-3-nitropyridine-2-carbonitrile

Heat a solution of 2-amino-5-bromo-3-nitropyridine (2.18 g, 10 mmol), cuprous cyanide (1.33 g,

15 mmol) and tgrt-butylnitrite (2.0 mL, 15 mmol) in acetonitrile (50 mL) to 60°C for 2 hours. Cool the solution and partition between ethyl acetate (100 mL) and saturated aqueous NaHC0₃ (100 mL). Extract the aqueous solution with ethyl acetate (2 x 50 mL), wash with water (100 mL), brine (100 mL), dry (MgSO₄) and evaporate. Purify the solid by flash chromatography on silica gel (25% ether / 75% hexane) to obtain the title compound as a pale yellow solid (934 mg, 41%).

2. 5-(3-Methyl(2-pyridyl))-3-nitropyridine-2-carbonitrile

Heat a solution of 5-bromo-3-nitropyridme-2-carbonitrile (228 mg, 1.0 mmol), tetralds(triphenylphosphine)palladium(0) (15 mg), 3-methyl-2-pyridylzinc bromide (0.5 M in THF, 3 mL, 1.5 mmol) in THF (5 mL) to 60°C for 2 hours. Cool the solution and partition between ethyl acetate (10 mL) and saturated aqueous NaHCO_. (10 mL). Extract the aqueous solution with ethyl acetate (2 x 15 mL), wash with water (10 mL), brine (10 mL), dry (MgSO₄) and evaporate to obtain the title compound as a pale yellow solid (211 mg, 88%).

3. 3-Amino-5-(3-methyl(2-pyridyl))pyήdine-2-carboxamide

Heat a solution of 5-(3-methyl(2-pyridyl))-3-nitropyridine-2-carbonitrile (1 g, 4.1 mmol), iron

(2.3 g, 40 mmol) and calcium chloride (560 mg, 5 mmol) in ethanol (15 mL) and water (4 mL) to reflux for 1 hour. Cool the mixture, filter through Celite and wash with ethyl acetate. Evaporate the filtrate and re-dissolve the residue in ethyl acetate, wash with water and then with brine, dry (MgSO₄) and evaporate to obtain the title compound as a pale yellow solid (880 mg, 94%). 4. [7-(3-Methyl-pyridin-2-yl)-2-pyrrolidin-l-ylmethyl-pyrido[3,2-d]pyrimidin-4-yl]-(4- trifluoromethyl-phenyl)-amine

The title compound is prepared from 3-amino-5-(3-methyl(2-pyridyl))pyridine-2-carboxamide in a manner analogous to that used for the preparation of [2-pyrrolidin-l-ylmethyl-7-(3-trifluoromethyl- pyridin-2-yl)-qumazolin-4-yl]-(4-trifluoromethyl-phenyl)-amine (Example 2.A, steps 6 to 9).

H. Additional Representative Substituted 2-Aminoalkyl-Ouinazolin-4-ylamine Analogues

Those having skill in the art will recognize that the starting materials may be varied and additional steps employed to produce other compounds encompassed by the present invention. Compounds listed in Table NI were prepared using the above methods, with readily apparent modifications.

Table VI Representative Substituted 2-Aminoalkyl-quinazolin-4-ylamine Analogues

EXAMPLE 3 Preparation of Representative Compounds This Example illustrates the preparation of representative substituted 2-hydroxyalkyl-quinazolin- 4-ylamine analogues.

A. [2-Isopropoxymethyl-7-(3-tiifluoromethyl-pyridin-2-yl)-quinazolin-4-yll-(4-tjifluoromethyl-phenyl)- amine ester (compound 701)

4. 2-p-tolyl-3-trifluoromethyl-pyridine

To a de-gassed mixture of 2-chloro-3-(trifluoromethyl)-pyridine (70.1 mmol), j. -tolylboronic acid (70.6 mmol), and 2M Na₂CO₃ (175.0 mmol), in dimethyl ether (DME; 200 mL) under nitrogen add Pd(PPh₃) (2.8 mmol). Stir the mixture at 80°C overnight, concentrate, and extract with EtOAc. Dry over Na₂SO₄, concentrate under vacuum, and pass through a silica gel pad to give 2-j. -tolyl-3- trifluoromethyl-pyridine. 5. 2-(4-methyl-3-nitro-phenyl)-3-(trifluoromethyl)-pyridine

To a solution of 2-j9-tolyl-3-trifluoromethyl-pyridine (8.4 mmol) in H₂SO (6 mL) cautiously add fuming HNO₃ (2 ml). Stir the mixture for 60 minutes at room temperature. Pour the mixture onto ice- water (30 mL), extract with EtOAc, neutralize with 1 N NaOH, dry over Na₂SO₄, and concentrate under vacuum to obtain 2-(4-methyl-3-nitτo-phenyl)-3-(tτifluoromethyl)-pyridine.

6. 2-nitro-4-(3-trifluoromethyl-pyridin-2-yl)-benzoic acid

To a solution of 2-(4-methyl-3-nitro-phenyl)-3-(trifluoromethyl)-pyridine (7.1 mmol) in a mixture of pyridine (10 mL) and water (5 ml), add KMn0 (25.3 mmol) portionwise. Stir the mixture for 4 hours at 110°C, and then add another 25.3 mmol of KMnO₄ with 10 ml of water. Stir the mixture at 110°C over night. Cool to room temperature, and filter through celite pad. Concentrate the filtrate under vacuum, dilute with water, and wash the aqueous solution with EtOAc. Neutralize the aqueous solution with 2 N HCl and collect the precipitate to give 2-nitro-4(3 -trifluoromethyl-pyridin-2 -yl)-benzoic acid. 4. 2-nitro-4-(3-trifluoromethyl-pyridin-2-yl)-benzamide

Reflux a mixture of 2-amino-4(3-trifluoromethyl-pyridin-2-yl)-benzoic acid (25 g) with SOCl₂ (50 ml) for 4 hours and concentrate. Dissolve the residue in dichloromethane (DCM), cool with ice- water bath, pass NH₃ gas through the solution for 30 minutes, and stir for 15 minutes at room temperature. Concentrate and wash with water to give 2-nitro-4-(3-trifluoromethyl-pyridin-2-yl)- benzamide.

5. 2-amino-4-(3-trifluoromethyl-pyridin-2-yl)-benzamide

Hydrogenate 2-nitτo-4-(3-trifluoromethyl-pyridin-2-yl)-benzamide (l.Og, 0.0032 mol) with 50 psi of H₂ and 100 mg of 10% Pd/C in ethanol. After 16 hours, filter the mixture through celite and concentrate under reduced pressure to give 2-ammo-4-(3-trifluoromethyl-pyridin-2-yl)-benzamide as a solid.

6. 2-chloromethyl-7-(3-trifluoromethyl-pyridin-2-yl)-3H-quinazolin-4-one

Heat a solution of 2-amino-4-(3 -trifluoromethyl-pyridin-2 -yl)-benzamide (100 mg, 0.356 mmol) in 2-chloro- 1,1,1-trimethoxyethane (bp 138°C) at 130°C for 4 hours. Concentrate the mixture under reduced pressure to give 2-chloromethyl-7-(3-trifluoromethyl-pyridin-2-yl)-3H-quinazolin-4-one as an oil which crystallizes on standing. 7. 4-chloro-2-chloromethyl- 7-(3-trifluoromethyl-pyridin-2-yl)-quinazoline

Reflux a mixture of 2-chloromethyl-7-(3 -trifluoromethyl-pyridin-2 -yl)-37J-quinazolin-4-one

(obtained from the reaction above) and POCl₃ for 16 hours. Cool the mixture and concentrate under reduced pressure. Partition the residue between EtOAc and saturated NaHCO₃ solution. Wash the EtOAc portion -with additional NaHCO_. and then dry (Na₂SO ) and concentrate under reduced pressure. Filter the brown residue through 2 inches of silica gel (1:1 EtOAc/hexanes eluent) and concentrate under reduced pressure to give 4-chloro-2-chloromethyl-7 -(3 -trifluoromethyl-pyridin-2 -yl)-quinazoline.

8. [2-chloromethyl- 7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(4-trifluoro methyl- phenyl)-amine

Heat a mixture of 4-chloro-2-chloromethyl-7-(3-trifluoromethyl-pyridin-2-yl)- quinazoline (42 mg, 0.117 mmol) and 4-trifluoromethyl-aniline (19 mg, 0.117 mmol) in isopropyl alcohol (1 mL) at 75°C for 4 hours. Cool the mixture and wash the precipitate with isopropyl alcohol followed by ether to give [2-chloromethyl-7-(3-tτifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(4- trifluoromethyl-phenyl)-amine as the mono-HCl salt.

9. [2-Isopropoxymethyl-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(4-trifluoromethyl- phenyl)-amine

To a suspension of [2-chloromethyl-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(4- trifluoromethyl-phenyl)-amine hydrochloride (1.9 g, 0.0037 mol) in dry isopropanol (100 mL), add 20 equivalents of NaO-i-Pr (prepared from Na and isopropanol). Stir the pale yellow mixture at 60°C for 5 hours, cool and evaporate the solvent under reduced pressure. Partition the residue between ethyl acetate and water and wash the organic layer with water (IX). Dry the organic layer (Na₂SO₄) and concentrate to give [2-isopropoxymethyl-7-(3-1rifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(4-trifluoromethyl- phenyl)-amine as a foam.

B. 2-[4-(4-Trifluoromethyl-phenylamino)-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-2-yl]-ethanol (compound 702)

1. 3-Benzyloxy-propionic acid

Add sodium hydride (2.22 g, 60% dispersion in mineral oil, 55.4 mmol) in small portions to a cold (0°C) solution of benzyl alcohol (4.0 g, 37 mmol) in toluene (100 mL). Add ethyl 3- bromopropionate (8.0 g, 44 mmol) dropwise to the mixture, allow the resulting solution to warm to room temperature and stir for 1 hour. Quench the reaction with the addition of water until all bubbling ceases. Dilute the mixture with ethyl acetate (100 mL) and extract with water (100 mL) and brine (100 mL). Dry the organic extract over Na₂SO₄ and remove the solvent under reduced pressure to yield the crude ester as a clear oil. Dissolve the oil in methanol (20 mL) and 6 N NaOH (20 mL), stir for 1 hour, concentrate the mixture (~ 20 mL) and dilute with water (20 mL). Extract the aqueous mixture once with CH₂C1₂ (40 mL). Acidify the aqueous phase with cone. HCl, extract with EtOAc (3 x 50 mL), and dry the combined EtOAc extracts over Na₂SO . Remove the solvent under reduced pressure to yield the title compound as a clear oil (2.28 g, 34.0 %) that solidifies upon standing.

2. 2-(2-Benzyloxy-ethyl)-7-(3-trifluoromethyl-pyridiny-2-yl)-3H-quinazolin-4-one

Cool a solution of 3-benzyloxy-propionic acid (1.66 g, 9.19 mmol) in hexanes (40 mL) to 0°C and add oxalyl chloride (3.50 g, 27.6 mmol) dropwise. After the addition is completed, add DMF (2 drops) and stir the resulting mixture for 1 hour. Remove the solvent under reduced pressure and dissolve the crude acid chloride in dry THF (20 mL). In a separate flask, dissolve 2-amino-4-(3-trifluoromethyl- pyridin-2-yl)-benzamide (2.35 g, 8.37 mmol) in dry THF (40 mL) and pyridine (0.727 g, 9.19 mmol) and cool to 0°C. Add the solution containing the crude acid chloride dropwise to the second solution. Allow the mixture to warm to room temperature and stir for 1 hour. Add a solution of 10% NaOH_(aq) (20 mL) to the mixture and stir the solution for 1 hour. Concentrate the mixture (~20 mL), dilute with water (20 mL), and acidify with cone. HCl. Extract the resulting solution with EtOAC (3 x 50 mL). Wash the combined organic extracts with brine and dry over Na₂SO₄. Remove the solvent under reduced pressure to yield the title compound as a white solid (3.24 g, 82.9 %). 3. 2-(2-Benzyloxy-ethyl)4-chloro-7-(3-trifluoromethyl-pyridiny-2-yl)-quinazoline

Dissolve 2-(2-Benzyloxy-ethyl)-7-(3 -trifluoromethyl-pyridiny-2-yl)-3H-quinazolin-4-one (3.24 g, 7.62 mmol) in CHC1₃ (40 mL) and 2,6-lutidine (2.45 g, 22.9 mmol). Add phosphorous oxycloride

(1.77 mL, 19.0 mmol) dropwise and heat the resulting solution to reflux for 18 hours. Cool the solution and remove the solvent under reduced pressure. Partition the crude residue between EtOAc (200 mL) and saturated NaHCO_{3 (aq)} (200 mL). Remove the organic phase and extract the aqueous phase with EtOAc (200 mL). Combine the two organic extracts, wash with brine (200 mL), and dry over Na₂SO₄. Remove the solvent to yield the title compound as a light brown solid (2.47 g, 73.1 %).

4. [2-(2-Benzyloxy-ethyl)-7-(3-trifluoromethyl-pyridiny-2-yl)-quinazolin-4-yl]-(4- trifluoromethyl-phenyl)-amine

Dissolve 2-(2-Benzyloxy-ethyl)-4-chloro-7-(3 -trifluoromethyl-pyridiny-2-yl)-quinazoline (2.47 g, 5.57 mmol) in a solution of acetonitrile (50 mL) and 4-trifluoromethyl-aniline (0.986 g, 6.12 mmol).

Heat the mixture to 80°C for 2 hours. A white precipitate forms. Cool the solution in an ice bath and add diethyl ether (25 mL). Filter off the white precipitate and dry in a vacuum oven to yield the title compound as the mono-hydrochloride salt (2.96 g, 87.8 %).

5. 2-[4-(4-Trifluoromethyl-phenylamino)-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-2-yl]- ethanol

Dissolve [2-(2-Benzyloxy-ethyl)-7-(3-trifluoromethyl-pyridiny-2-yl)-quinazolin-4-yl]-(4- trifluoromethyl-phenyl)-amine hydrochloride (2.96 g, 4.89 mmol) in MeOH (150 mL) and add 10%) Pd/C (200 mg). Hydrogenate the mixture at 50 p.s.i. at 60°C for 8 hours. Quicldy filter the mixture through Celite and wash the Celite filter cake with hot MeOH (200 mL). Remove the solvent under reduced pressure to yield the mono-hydrochloride salt of title compound as a white solid (1.75 g, 69.5 %). Mass Spec. 478.1. C. 2-(2-methoxy-ethyl)-7-(3-trifluoromethyl-pyridm-2-yl)-quinazolin-4-yl]-(4-t:ifluoromethyl-phenyl)- amine (compound 703)

1. 2-(2-methoxy-ethyl)- 7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-ol

To a solution of 2-amino-4-(3-trifluoromethyl-pyridin-2-yl)-benzamide (3.56 mmol) and pyridine (3.91 mmol) in THF (20 ml), add 4-methoxy-butyryl chloride (3.91 mmol). Stir the mixture 20 minutes at room temperature, add 20 ml of 20% NaOH, stir for 60 minutes at 50°C. Concentrate, add water, filter, acidify to pH=6, collect the precipitate to obtain 2-(3-benzyloxy-propyl)7-(3- trifluoromethyl-pyridin-2-yl)-quinazolin-4-ol.

2. 2-(2-methoxy-ethyl)-4-chloro-7-(3-trifluoromethyl-pyridin-2-yl)-quinazoline

Using procedures analogous to those already described, 2-(2-methoxy-ethyl)-4-chloro-7-(3- trifluoromethyl-pyridin-2-yl)-quinazoline is prepared from 2-(2-methoxy-ethyl)-7-(3 -trifluoromethyl- pyridin-2 -yl)-quinazolin-4-ol.

3. [2-(2-ιnethoxy-ethyl)-7-(3-trifluoromethyl-pyridin-2-yl)-quina∑olm-4-yl]-(4-trifluoromethyl- phenyl)-amine

Using procedures analogous to those already described, [2-(2-methoxy-ethyl)-7-(3- trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(4-trifluoromethyl-phenyl)-amine is prepared from 2-(2- methoxy-ethyl)-4-chloro-7-(3-trifluoro- methyl-pyridin-2-yl)-quinazoline. Mass Spec. 492.1. P. 3-[4-(4-trifluoromethyl-ρhenylamino)-7-(3-1τifluoro-methyl-pyridin-2-yl)-quinazolin-2-yll-propan-l- ol (compound 704)

1. 2-(3-benzyloxy-propyl)- 7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-ol

To a solution of 2-amino-4-(3-trifluoromethyl-pyridin-2-yl)-benzamide (3.56 mmol) and pyridme (3.91 mmol) in THF (20 ml) add 4-benzyloxy-butyryl chloride (3.91 mmol). Stir the mixture 20 minutes at room temperature, add 20 ml of 20% NaOH, stir for 60 minutes at 50°C. Concentrate, add water, filter, acidify to pH=6, collect the precipitate to obtain 2-(3-benzyloxy-propyl)-7-(3- trifluoromethyl-pyridin-2-yl)-quinazolin-4-ol.

2. 2-(3-benzyloxy-propyl)-4-chloro-7-(3-trifluoromethyl-pyridin-2-yl)-quinazoline

Using procedures analogous to those already described 2-(3-benzyloxy-propyl)-4-chloro-7-(3- trifluoromethyl-pyridin-2-yl)-quinazoline can be prepared from 2-(3-benzyloxy-propyl)-7-(3- trifluoromethyl-pyridin-2-yl)-quinazolin-4-ol.

3. [2-(3-benzyloxy-propyl)- 7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(4- trifluoromethyl-phenyl) -amine

Using procedures analogous to those already described, [2-(3-benzyloxy-propyl)-7-(3- trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(4-trifluoromethyl-phenyl)-amine is prepared from 2-(3- benzyloxy-propyl)-4-chloro-7-(3-trifluoromethyl-pyridin-2-yl)-quinazoline. 4. 3-[4-(4-trifluorornethyl-phenylamino)-7-(3-trifluoro-methyl-pyridin-2-yl)-qumazolin-2-yl]- propan-1-ol

Hydrogenate the mixture of 2-(3-benzyloxy-propyl)-4-chloro-7-(3-trifluoromethyl-pyridin-2-yl)- quinazoline (0.5 mmol) and 10% Pd-C in EtOH (100 ml) at 50 psi for 30 hours. Filter, concentrate, and chromatograph to give 3-[4-(4-trifluoromethyl-phenylamino)-7-(3-trifluoro-methyl-pyridin-2-yl)- quinazolin-2-yl]-propan-l-ol. Mass Spec. 492.1.

E. 7-(3-tiifluoromethyl-pyridm-2-yl)-2-methoxymethyl-pyrido[3.2-d1pyrimidin-4-yl]-(4-trifluoromethyl- phenvD-amine (compound 705)

1. 6 '-Methoxy- 3-trifluoromethyl- [2, 3 'Jbipyridinyl

Heat a mixture of 2-chloro-3-trifluoromethylpyridine (37 g, 0.2 mol), 2-methoxypyridine-5- boronic acid (32 g, 0.21 mol), tetralds(triphenylphosphine)palladium(0) (9 g, 7 mmol) and 2M potassium carbonate (150 mL) in toluene (500 mL) under a nitrogen atmosphere at 90°C for 8 hours. Cool the reaction mixture and separate the layers. Extract the aqueous layer with ethyl acetate (2 x 250 mL) and wash the combined organics with 4M sodium hydroxide (250 mL), water (250 mL), and brine (250 mL). Dry (MgSO₄) and concentrate under reduced pressure. Purify the resulting oil by flash chromatography on silica gel (50% ether/ 50% hexane) to give the title compound (48.2 g, 95%) as a colorless oil. 2. 3-Trifluoromethyl-l 'H-[2,3 ']bipyridinyl-6'-one

Heat 6'-Methoxy-3-trifluoromethyl-[2,3']bipyridinyl (41 g, 0.16 mol) in 30% HBr/AcOH (100 mL) to reflux for 1 hour. Cool the mixture, filter and wash the precipitate with ether (100 mL). Transfer the precipitate into 10M sodium hydroxide (500 mL) and stir for 1 hour. Treat the solution with hydrochloric acid until the solution is pH 7. Collect the white solid by filtration and air dry to give the title compound (36 g, 93%) as a white solid.

3. 5 '-Nitro- 3-trifluoromethyl-l 'H-[2,3 ']bipyridinyl-6'-one

To a solution of 3-trifluoromethyl-rH-[2,3']bipyridinyl-6'-one (25 g, 0.1 mol) in concentrated sulfuric acid (100 mL) at 0°C, add dropwise a solution of fuming nitric acid (35 L) and concentrated sulfuric acid (10 mL). Heat the reaction mixture to 70 °C for 1 hour, cool and pour onto ice (500 mL). Filter the mixture and treat the filtrate with 10 M sodium hydroxide until the solution is at pH 4-5. Collect the precipitate by filtration and air dry to give the title compound (26.2 g, 92%) as a white solid. 4. 6 '-Chloro-5 '-nitro-3-trifluoromethyl-[2,3 'J bipyridinyl

Heat a solution of 5'-nitro-3-trifluoromethyl-l'H-[2,3']bipyridinyl-6'-one (25 g, 0.088 mol), thionyl chloride (300 mL) and DMF (3 mL) to reflux for 4 hours. Remove the volatiles by rotary evaporation and partition the residue between ethyl acetate (350 mL) and saturated sodium bicarbonate solution (250 mL). Extract the aqueous layer with further ethyl acetate (250 mL) and wash the combined organics with brine (250 mL). Dry (MgS0₄) and concentrate under reduced pressure to give the title compound (25 g, 93%) as a yellow oil.

5. 6 '- Chloro- 3 -trifluoromethyl- [2, 3 'Jbipyridinyl-5 '-ylamine

To a solution of 6'-chloro-5'-nitro-3-trifluoromethyl-[2,3']bipyridinyl (25 g, 0.082 mol) and calcium chloride (llg, 0.1 mol) in ethanol (300 mL) and water (50 mL), add iron powder (45 g, 0.82 mol). Heat the solution to reflux for 1.5 hours, cool and filter through Celite. Concentrate the mixture under reduced pressure, re-dissolve in ethyl acetate (300 mL) and wash with brine (200 mL). Concentrate the solution under reduced pressure and purify by flash chromatography on silica gel (50% ether/ 50% hexane) to give the title compound (19 g, 85%) as a pale yellow solid. 6. 3-Amino-5-[3-(trifluoromethyl)(2-pyridyl)]pyridine-2-carboxamide

Heat a solution of 6'-chloro-3-trifluoromethyl-[2,3']bipyridinyl-5 '-ylamine (25 g, 0.091 mol), zinc cyanide (6.75 g, 0.058 mol), tris[dibenzylidineacetone]di-palladium (pd₂(dba)₃; 2.63 g, 2.86 mmol), and l,l'-bis(diphenylphosphino)ferrocene (DPPF; 3.16g, 5.72 mmol) in DMF (250 mL) and water (2.5 mL), under a nitrogen atmosphere, at 120°C for 1 hour. Add water (30 mL) and heat the solution at 120°C for a further 4 hours to complete the hydrolysis. Cool the reaction to 0°C and add a solution of saturated ammonium chloride (200 ml), water (200 mL) and concentrated ammonium hydroxide (50 mL). After stirring at 0°C for 1 hour, filter the yellow precipitate, and wash with water (200 mL) and a 1:1 mixture of ether-hexane (200 mL). Air dry the solid, and then dry in a vacuum oven to give (23g, 90%) ofthe title compound.

7. 2-(Chloromethyl)-7-[3-(trifluoromethyl)(2-pyridyl)]-3-hydropyridino[3,2-d]pyrimidin-4-one

Heat a solution of 3-amino-5-[3-(trifluoromethyl)(2-pyridyl)]pyridine-2-carboxamide (23 g, 81.5 mmol) and 2-chloro 1,1,1-trimethoxyethane (250 mL) at 130°C for 1 hour. Remove the volatiles by evaporation and friturate the solid (50% ether/ 50% hexane) to give the title compound as a light brown solid (21 g, 76%).

8. 4-Chloro-2-chloromethyl-7-(3-chloro-pyridin-2-yl) -pyrido [3, 2-d] pyrimidine

Heat a solution of 2-(chloromethyl)-7-[3-(trifluoromethyl)(2-pyridyl)]-3-hydropyridino[3,2- d]pyrimidin-4-one (2.49 g, 7.31 mmol), phosphorous oxychloride (10 mL), 2,6-lutidine (2.13 mL, 18.3 mmol) and toluene to reflux for 8 hours. Remove the solvent and partition the crude residue between EtOAc (150 mL) and H₂O (150 mL). Remove the organic phase and extract the aqueous phase with EtOAc (150 rnL). Combine the organic extractions, wash with saturated NaHC0₃(aq) (150 mL) and brine (150 mL), and dry over Na₂SO₄. Remove the solvent to yield the title compound as a light brown solid (2.30 g, 87.6 %). 9. [2-(2-Chloroιnethyl)-7-(3-trifluoroτnethyl-pyridiny-2-yl)-quirιazolin-4-yl] -(4-trifluoromethyl- phenyl)-amine

Dissolve 4-Chloro-2-chloro-methyl-7-(3-chloro-pyridin-2-yl)-pyrido[3,2-d]pyrimidine (2.30 g, 6.40 mmol) in a solution of acetonitrile (20 mL) and 4-trifluoromethyl aniline (1.13 g, 7.04 mmol). Heat the mixture at 80°C for 18 hours. Cool the mixture to 0°C and dilute with diethyl ether (20 mL). The mono-hydrochloride salt of the title compound forms a light brown precipitate (2.85 g 85.6%), which is removed by filtration and dried in a vacuum oven.

10. 7-(3-trifluoromethyl-pyridin-2-yl)-2-methoxymethyl-pyrido[3,2-d]pyrimidin-4-yl]-(4- trifluoromethyl-phenyl)-amine

Treat [2-(2-Chloromethyl)-7-(3-trifluoromethyl-pyridiny-2-yl)-quinazolin-4-yl]-(4- trifluoromethyl-phenyl)-amine with NaOMe as described in Example l.A-9 above. This affords 7-(3- trifluoromethyl-pyridin-2-yl)-2-methoxymethyl-pyrido[3,2-d]pyrimidin-4-yl]-(4-trifluoromethyl- phenyl)-amine as a solid. Mass Spec. 479.1.

F. 7-(3-methyl-pyridin-2-yl)-2-methoxymethyl-pyrido[3.2-d]pyrimidin-4-yl]-(4-trifluoromethyl-phenyl)- amine (compound 706)

1. 5-Bromo-3-nitropyridine-2-carbonitrile

Heat a solution of 2-amino-5-bromo-3-nitropyridine (2.18 g, 10 mmol), cuprous cyanide (1.33 g,

15 mmol) and tert-butylnitrite (2.0 mL, 15 mmol) in acetonitrile (50 mL) at 60°C for 2 hours. Cool the solution and partitioned between ethyl acetate (100 mL) and saturated aqueous NaHCO_. (100 mL).

Extract the aqueous layer with ethyl acetate (2 x 50 mL), wash with water (100 mL) and brine (100 mL), dry (MgSO₄) and evaporate. Purify the solid by flash chromatography on silica gel (25% ether / 75% hexane) to give the title compound as a pale yellow solid (934 mg, 41%>).

4. 5-(3-Methyl(2-pyridyl))-3-nitropyridine-2-carbonitrile

Heat a solution of 5-bromo-3-nitropyridine-2-carbonitrile (228 mg, 1.0 mmol), tetrakis(triphenylphosphine)palladium(0) (15 mg), 3-methyl-2-pyridylzinc bromide (0.5 M in THF, 3 mL, 1.5 mmol) in THF (5 mL) at 60°C for 2 hours. Cool the solution and partition between ethyl acetate (10 mL) and saturated aqueous NaHCO₃ (10 mL). Extract the aqueous layer with ethyl acetate (2 x 15 L), wash with water (10 mL) and brine (10 mL), dry (MgSO₄) and evaporate to give the title compound as a pale yellow solid (211 mg, 88%).

5. 3-Amino-5-(3-methyl(2-pyridyl))pyridine-2-carboxamide

Heat a solution of 5-(3-methyl(2-pyridyl))-3-nitOpyridine-2-carbonitrile (1 g, 4.1 mmol), iron

(2.3 g, 40 mmol) and calcium chloride (560 mg, 5 mmol) in ethanol (15 mL) and water (4 mL) to reflux for 1 hour. Cool the mixture, filter through Celite and wash with ethyl acetate. Evaporate the filtrate and re-dissolve the residue in ethyl acetate. Wash with water and brine, dry (MgSO₄) and evaporate to give the title compound as a pale yellow solid (880 mg, 94%).

4. 7-(3-methyl-pyridin-2-yl)-2-methoxymethyl-pyrido[3,2-d]pyrimidin-4-yl]-(4-trifluoromethyl- phenyl)-amine

The title compound is prepared from 5'-amino-3-methyl-[2,3']bipyridinyl-6'-carboxylic acid amide using procedures analogous to those described in examples A, B, D, E, G and H. G. 7-(3 -trifluoromethyl-pyridin-2-yl)-2-methoxymethyl-pyrido [3.2-dlpyrimidin-4-yl] -(4- trifluoromethyl-phenvD-amine (compound 707)

1. 7-(3-trifluoromethyl-pyridin-2-yl)-2-methoxymethyl-3H-pyrido[3,2-d]pyrimidin-4-one

Treat a solution of 3-amino-5-[3-(chloro-pyridin-2-yl)]pyridine-2-carboxamide (340 mg, 1.21 mmol) in THF (5 mL) and pyridine (0.11 mL) with methoxy-acetyl chloride (O.llmL, 144 mg, 1.33 mmol). Stir the mixture for 3 hours at room temperature. Then, add 5 N NaOH (10 mL) and stir the solution for an additional 18 hours. Concentrate the solution (~ 5 mL) and acidify with cone. HCl. Extract the aqueous mixture with EtOAc (3 x 25 mL), and dry the combined organic extracts over Na₂S0 . Remove the solvent under reduced pressure to yield the title compound as a white solid.

2. 4-Chloro-7-(3-trifluoromethyl-pyridin-2-yl)-2-methoxymethyl-pyrido[3,2-d]pyrimidine

Dissolve 7-(3-1xifluoromethyl-pyridin-2-yl)-2-methoxymethyl-3H-pyrido[3,2-d]pyrimidin-4-one (276 mg, 0.822 mmol) in CHC1₃ (25 mL) and 2,6-lutidine (294 mg, 2.74 mmol). Add phosphorous oxycloride (0.255 mL, 2.74 mmol) dropwise and heat the resulting solution to reflux for 24 hours. Cool the solution and remove the solvent under reduced pressure. Partition the crude residue between EtOAc (50 mL) and saturated NaHCO_{3 (aq)} (50 mL). Remove the organic phase and extract the aqueous phase with additional EtOAc (50 mL). Combine the two organic extracts, wash with brine (100 mL), and dry over Na₂SO . Remove the solvent to yield the title compound as a light brown solid. 3. 7-(3-trifluoromethyl-pyridin-2-yl)-2-methoxymethyl-pyrido[3,2-d]pyrimidin-4-yl]-(4- trifluoromethyl-phenyl) -amine

Dissolve 4-Chloro-7-(3-frifluoromethyl-pyridin-2-yl)-2-methoxymethyl-pyrido[3,2-d]pyrimidine (30 mg, 0.0934 mmol) into a solution of acetonitrile (3 mL) and 4-trifluoromethyl-aniline (18.0 mg, 0.112 mmol). Heat the mixture to 80°C for 16 hours. Cool the reaction mixture in an ice bath and add diethyl ether (3 mL). Filter off the off-white precipitate and dry in a vacuum oven to yield the title compound as the mono-hydrochloride salt. Mass Spec. 479.1.

H. [7-(3-Chloro-pyridin-2-yl)-2-methoxymethyl-pyrido[2.3-d1pyriιτύdin-4-yll-(4-trifluoromethyl- phenvD-amine (compound 708)

1. 2-Acetyl-3-chloropyridine

Dissolve 3-chloro-2-cyanopyridine (10.0 g, 0.072 mol, Chem. Pharm. Bull. (1985) 33:565-571) in anhydrous THF (200 mL) under N atmosphere and cool in an ice bath. Add drop wise 3.0 M MeMgl in diethyl ether (48 ml, 0.14 mol) to the reaction mixture and stir in an ice bath for 2 hours. Pour the reaction mixture over ice cold water, acidify the mixture with 2.0 N aq. HCl to pH 2 to 3. Extract the reaction mixture with EtOAc (3 x 100 mL) and dry over anhydrous MgSO₄. Filter, concentrate under vacuum and then filter through a pad of silica gel using 20% ethyl acetate / hexane as eluent. Removal of solvent under reduced pressure gives pure 2-acexyl-3-chloropyridine as oil. 2. l-(3-Chloro-pyridin-2-yl)-3-dimethylaminopropenone

Heat 2-acetyl-3-chloropyridine (0.77 g, 5.0 mmol) with N,N-dimethylformamide dimexylacetal (3.0 g) at 105°C for 20 hours. Concentrate under reduced pressure to give l-(3-chloro-pyridin-2-yl)-3- dimethylaminopropenone as oil. 3. 2-Amino-4-(3-chloro-pyridin-2-yl)-benzonitrile

Heat a solution of l-(3-Chloro-pyridin-2-yl)-3-dimethylaminopropenone (1.05 g, 5 mmol), 3- ammo-3-methoxy-acrylonitrile hydrochloride (1.35 g, 10 mmol) and ammonium acetate (2.2 g, 15.0 mmol) in ethanol (25 mL) at reflux for 20 hours. Cool the mixture and concentrate under reduced pressure to give dark oil. Dissolve the residue in EtOAc / water (100 mL). Extract the aqueous solution with EtOAc, wash the EtOAc with brine, dry (MgSO ) and concentrate under reduced pressure to give 2-amino-4-(3-chloro-pyridin-2-yl)-benzonitrile as a brown solid.

4. 6-Amino-3'-chloro-[2,2]' bipyridinyl-5-carboxylic acid amide

Cool concentrated sulfuric acid (10 mL) in an ice bath under nitrogen atmosphere. Add in portions 2-amino-4-(3-chloro-pyridin-2-yl)-benzonitrile (1.0 g, 4.3 mmol) over a period of 15 minutes. Stir at room temperature overnight. Pour the reaction mixture over ice, adjust the pH to 10 using IO N aq. NaOH, filter the solid, wash the solid with water and dry under vacuum to give 6-amino-3'-chloro- [2,2']bipyridinyl-5 -carboxylic acid amide as a yellow solid.

5. 7-(3-Chloro-pyridin-2-yl)-2-methoxymethyl-3H-pyrido[2,3-d]pyrimidin-4-one

Dissolve 6-amino-3'-chloro-[2,2']bipyridinyl-5 -carboxylic acid amide (0.5 g, 2.02 mmol) in anhydrous THF (10 mL) under N atmosphere. Add drop wise pyridine (0.36 g, 4.04 mmol) and methoxyacetyl chloride (0.48 g, 4.04 mmol) to the reaction mixture and stir at room temperature overnight. Add 10 % aq. NaOH (10 mL) and reflux for 4 hours. Concentrate in vacuum, adjust the pH to 6.0 using AcOH, collect the solid by filtration and dry under vacuum to give 7-(3-chloro-pyridin-2-yl)- 2-methoxymethyl-3H-pyrido[2,3-d]pyrimidin-4-one as a white solid. 6. 4-Chloro- 7-(3-chloro-pyridin-2-yl)-2-methoxymethyl-pyrido[2, 3-d] pyrimidine

Reflux a mixture of 7-(3-chloro-pyridin-2-yl)-2-methoxymethyl-3H-pyrido[2,3-d]pyrimidin-4- one (0.25 g), 2,6-lutidine (0.44 g), and POCl₃ (0.51 g) in CHC1₃ (5 mL) for 20 hours. Cool the mixture and concentrate under reduced pressure. Partition the residue between EtOAc and saturated NaHCO₃ solution. Wash the EtOAc portion with additional NaHCO₃ and then dry (Na₂SO₄) and concentrate under reduced pressure. Filter the brown residue through 2 inches of silica gel (1:1 EtOAc/hexanes eluent) and concentrate under reduced pressure to give 4-chloro-7-(3-chloro-pyridin-2-yl)-2-methoxymethyl- pyrido[2,3-d]pyrimidine. 7. [7-(3-Chloro-pyridin-2-yl)-2-methoxymethyl-pyrido[2,3-d]pyrimidi}i-4-yl]-(4-trifluoromethyl- phenyl)-amine

Heat a mixture of 4-chloro-7-(3-chloro-pyridin-2-yl)-2-methoxymethyl-pyrido[2,3-d]pyrimidine (0.1 mmol) and 4-trifluoromethyl-aniline (16.1 mg, O.lmmol) in AcCN (1 mL) at 80°C for 24 hours.

Cool the mixture and wash the precipitate with ether to give [7-(3-chloro-pyridin-2-yl)-2- methoxymethyl-pyrido[2,3-d]pyrimidin-4-yl]-(4-trifluoromethyl-phenyl)-amine as the mono-HCl salt.

Mass Spec. 445.1.

I. 2-Methox'yτnethyl-7-(3-methylpyridin-2-yl)-quinazolin-4-yl)-(4-trifluoromethylphenyl)-amine (compound 709)

1. 7-bromo-2-methoxymethylquinazolin-4-yl)-(4-trifluorometliylphenyl)-amine

Heat a mixture of 7-bromo-2-chloromethylquinazolin-4-yl)-(4-trifluoromethylphenyl)-amine (from Example C, 200 mg, 0.48 mmol), 4.4M sodium methoxide in methanol (2.4 mL), and methanol (1 mL) to 60°C for 4 hours. Cool to room temperature and evaporate the mixture. Dilute with EtOAc (10 mL) and wash 2X with water (10 mL each). Dry the organic layer (Na₂S0₄) and evaporate. Purify by preparative TLC (3:1 hexanes:EtOAc) to obtain 2-methoxymethyl-7-pyridin-4-yl-quinazolin-4-yl)-(4- xrifluoromethylphenyl)-amine (225 mg) as a yellow solid. 2. [2-Methoxymethyf7-(3-methylpyridin-2-yl)-quinazolin-4-yl)-(4-trifluoromethylphenyl)-amine

Heat a mixture of 2-methoxymethyl-7-pyridin-4-yl-quinazolin-4-yl)-(4-trifluoromethylphenyl)- amine (100 mg, 0.243 mmol), 3-methyl-2-pyridylzinc bromide (1 mL of a 0.5M THF solution), tetrakis(triphenylphosphinepalladium(0) (50 mg, 0.043 mmol) in 1,2-dimethoxymethane (5 mL) for 3 hours at 80°C under nitrogen. Cool to room temperature and dilute with EtOAc (10 mL). Wash with water (2 x 10 mL) and dry the organic layer (Na₂SO₄) and evaporate. Purify by preparative TLC to obtain [2-methoxymethyl-7-(3-methylpyridin-2-yl)-quinazolin-4-yl)-(4-trifluoromethylphenyl)-amine (38 mg) as an off-white solid.

J. Additional Representative Substituted 2-Hydroxyalkyl-Qumazolin-4-ylamine Analogues

Compounds listed in Table VII were prepared using the above methods, with readily apparent modifications.

Table VII Representative Substituted 2-Hydroxyalkyl-quinazolin-4-ylamine Analogues

EXAMPLE 4 VRl -Transfected Cells and Membrane Preparations This Example illustrates the preparation of VRl -transfected cells and VR1- containing membrane preparations for use in capsaicin binding assays (Example 5). A cDNA encoding full length human capsaicin receptor (SEQ ID NO:l, 2 or 3 of

U.S. Patent No. 6,482,611) was subcloned in the plasmid pBK-CMV (Stratagene, La Jolla, CA) for recombinant expression in mammalian cells.

Human embryonic kidney (HEK293) cells were transfected with the pBK-CMV expression constract encoding the full length human capsaicin receptor using standard methods. The transfected cells were selected for two weeks in media containing G418 (400 μg/ml) to obtain a pool of stably transfected cells. Independent clones were isolated from this pool by limiting dilution to obtain clonal stable cell lines for use in subsequent experiments.

For radioligand binding experiments, cells were seeded in T175 cell culture flasks in media without antibiotics and grown to approximately 90% confluency. The flasks were then washed with PBS and harvested in PBS containing 5 mM EDTA. The cells were pelleted by gentle centrifugation and stored at -80°C until assayed.

Previously frozen cells were disrupted with the aid of a tissue homogenizer in ice-cold HEPES homogenization buffer (5mM KC1 5, 5.8mM NaCl, 0.75mM CaCl₂, 2mM MgCl₂, 320 mM sucrose, and 10 mM HEPES pH 7.4). Tissue homogenates were first centrifuged for 10 minutes at 1000 x g (4°C) to remove the nuclear fraction and debris, and then the supernatant from the first centrifugation is ftirther centrifuged for 30 minutes at 35,000 x g (4°C) to obtain a partially purified membrane fraction. Membranes were resuspended in the HEPES homogenization buffer prior to the assay. An aliquot of this membrane homogenate is used to determine protein concentration via the Bradford method (BIO-RAD Protein Assay Kit, #500-0001, BIO-RAD, Hercules, CA).

EXAMPLE 5 Capsaicin Receptor Binding Assay

This Example illustrates a representative assay of capsaicin receptor binding that may be used to determine the binding affinity of compounds for the capsaicin (VRl) receptor. Binding studies with [³H] Resiήiferatoxin (RTX) are carried out essentially as described by Szallasi and Blumberg (1992) J. Pharmacol. Exp. Ter. 262: 883-888. In this protocol, non-specific RTX binding is reduced by adding bovine alphai acid glycoprotein (100 μg per tube) after the binding reaction has been terminated. [³H] RTX (37 Ci/mmol) is synthesized by and obtained from the Chemical

Synthesis and Analysis Laboratory, National Cancer Institute-Frederick Cancer Research and Development Center, Frederick, MD. [³H] RTX may also be obtained from commercial vendors (e.g., Amersham Pharmacia Biotech, Inc.; Piscataway, NJ).

The membrane homogenate of Example 4 is centrifuged as before and resuspended to a protein concentration of 333 μg/ml in homogenization buffer. Binding assay mixtures are set up on ice and contained [³H]RTX (specific activity 2200 mCi/ml), 2 μl non-radioactive test compound, 0.25 mg/ml bovine serum albumin (Cohn fraction V), and 5 x 10⁴ - 1 x 10⁵ VRl -transfected cells. The final volume is adjusted to 500 μl (for competition binding assays) or 1,000 μl (for saturation binding assays) with the ice- cold HEPES homogenization buffer solution (pH 7.4) described above. Non-specific binding is defined as that occurring in the presence of 1 μM non-radioactive RTX (Alexis Corp.; San Diego, CA). For saturation binding, [³H]RTX is added in the concentration range of 7 - 1,000 pM, using 1 to 2 dilutions. Typically 11 concentration points are collected per saturation binding curve. Competition binding assays are performed in the presence of 60 pM [ H]RTX and various concentrations of test compound. The binding reactions are initiated by transfening the assay mixtures into a 37°C water bath and are terminated following a 60 minute incubation period by cooling the tubes on ice. Membrane-bound RTX is separated from free, as well as any alphai-acid glycoprotein-bound RTX, by filtration onto WALLAC glass fiber filters (PERKIN-ELMER, Gaithersburg, MD) which were pre-soaked with 1.0% PEI (polyethyleneimine) for 2 hours prior to use. Filters are allowed to dry overnight then counted in a WALLAC 1205 BETA PLATE counter after addition of WALLAC BETA SCENT scintillation fluid.

Equilibrium binding parameters are determined by fitting the allosteric Hill equation to the measured values with the aid of the computer program FIT P (Biosoft, Ferguson, MO) as described by Szallasi, et al. (1993) J. Pharmacol. Exp. Ther. 266:678- 683. Compounds provided herein generally exhibit Kj values for capsaicin receptor of less than 4 μM, preferably less than 1 μM, 100 nM, 50 nM, 25 nM, 10 nM or InM in this assay.

EXAMPLE 6 Calcium Mobilization Assay

This Example illustrates a representative calcium mobilization assay for use in monitoring the response of cells expressing capsaicin receptor to capsaicin and other vanilloid ligands of the capsaicin receptor, as well as for evaluating test compounds for agonist and antagonist activity. Cells transfected with expression plasmids (as described in Example 4) and thereby expressing human capsaicin receptor are seeded and grown to 70-90% confluency in FALCON black-walled, clear-bottomed 96-well plates (#3904, BECTON- DICKINSON, Franklin Lakes, NJ). The culture medium is emptied from the 96 well plates and FLUO-3 AM calcium sensitive dye (Molecular Probes, Eugene, OR) is added to each well (dye solution: 1 mg FLUO-3 AM, 440 μL DMSO and 440 μl 20% pluronic acid in DMSO, diluted 1:250 in Krebs-Ringer HEPES (KRH) buffer (25 mM HEPES, 5 mM KC1, 0.96 mM NaH₂PO₄, 1 mM MgSO₄, 2 mM CaCl₂, 5 mM glucose, 1 mM probenecid, pH 7.4), 50 μl diluted solution per well). Plates are covered with aluminum foil and incubated at 37°C for 1-2 hours in an environment containing 5% CO₂. After the incubation, the dye is emptied from the plates, and the cells are washed once with KRH buffer, and resuspended in KRH buffer.

Agonist (e.g., olvanil, capsaicin, or RTX)-induced calcium mobilization is monitored using either FLUOROSKAN ASCENT (Labsystems, Franklin, MA) or FLIPR (fluorometric imaging plate reader system, Molecular Devices, Sunnyvale, CA) instruments. Varying concentrations of the antagonists ruthenium red or capsazepine (RBI; Natick, MA) are added to cells concunently with agonist (e.g., 25-50 nM capsaicin). For agonist-induced calcium responses, data obtained between 30 and 60 seconds after agonist application are used to generate the IC₅₀ values. KALEIDAGRAPH software (Synergy Software, Reading, PA) is used to fit the data to the equation: y=a*(l/(l+(b/x)^c)) to determine the IC₅₀ for the response. In this equation, y is the maximum fluorescence signal, x is the concentration of the agonist or antagonist, a is the E_max, b conesponds to the IC5₀ value and c is the Hill coefficient. To measure the ability of a test compound to antagonize (inhibit) the response of cells expressing capsaicin receptors to capsaicin or other vanilloid agonist, the IC₅₀ of capsaicin is first determined. An additional 20 μl of KRH buffer and 1 μl DMSO is added to each well of cells, prepared as described above. 100 μl capsaicin in KRH buffer is automatically transfened by the FLEPR instrument to each well. An 8-point concentration response curve, with final capsaicin concentrations of 1 nM to 3 μM, is used to determine capsaicin IC₅₀.

Test compounds are dissolved in DMSO, diluted in 20 μl KRH buffer so that the final concentration of test compounds in the assay well is between 1 μM and 5 μM, and added to cells prepared as described above. The 96 well plates containing prepared cells and test compounds are incubated in the dark, at room temperature for 0.5 to 6 hours. It is important that the incubation not continue beyond 6 hours. Just prior to determining the fluorescence response, 100 μl capsaicin in KRH buffer at twice the IC₅₀ concentration detennined from the concentration response curve is automatically added by the FLEPR instrument to each well of the 96 well plate for a final sample volume of 200 μl and a final capsaicin concentration equal to the EC₅Q. The final concentration of test compounds in the assay wells is between 1 μM and 5 μM. Typically cells exposed to one IC₅₀ of capsaicin exhibit a fluorescence response of about 10,000 Relative Fluorescence Units. Antagonists of the capsaicin receptor decrease this response by at least about 20%, preferably by at least about 50%, and most preferably by at least 80% as compared to matched control. The concentration of antagonist required to provide a 50% decrease is the IC₅₀ for the antagonist, and is preferably below 1 micromolar, 100 nanomolar, 10 nanomolar or 1 nanomolar.

The ability of a compound to act as an agonist of the capsaicin receptor is determined by measuring the fluorescence response of cells expressing capsaicin receptors, using the methods described above, in the absence of capsaicin, RTX, or other known capsaicin receptor agonists. Compounds that cause cells to exhibit fluorescence above background are capsaicin receptor agonists. Certain prefened compounds of the present invention are antagonists that are essentially free of agonist activity as demonstrated by the absence of detectable agonist activity in such an assay at compound concentrations below 4 nM, more preferably at concentrations below 10 μM and most preferably at concentrations less than or equal to 100 μM. EXAMPLE 7 Microsomal in vitro half-life This Example illustrates the evaluation of compound half-life values (t__/2 values) using a representative liver microsomal half-life assay. Pooled human liver microsomes are obtained from XenoTech LLC, 3800

Cambridge St., Kansas City, Kansas 66103 (catalog # H0610). Such liver microsomes may also be obtained from In Vitro Technologies (Baltimore, MD) or Tissue Transformation Technologies (Edison, NJ). Six test reactions are prepared, each containing 25 μl microsomes, 5 μl of a 100 μM solution of test compound, and 399 μl 0.1 M phosphate buffer (19 mL 0.1 M NaH₂PO₄, 81 mL 0.1 M Na₂HPO₄, adjusted to pH 7.4 with H₃PO₄). A seventh reaction is prepared as a positive control containing 25 μl microsomes, 399 μl 0.1 M phosphate buffer, and 5 μl of a 100 μM solution of a compound with known metabolic properties (e.g., DIAZEPAM or CLOZAPINE). Reactions are preincubated at 39°C for 10 minutes. CoFactor Mixture is prepared by diluting 16.2 mg NADP and 45.4 mg Glucose-

6-phosphate in 4 mL 100 mM MgCl . Glucose-6-phosphate dehydrogenase solution is prepared by diluting 214.3 μl glucose-6-phosphate dehydrogenase suspension (Boehringer-Manheim catalog no. 0737224, distributed by Roche Molecular Biochemicals, Indianapolis, EN) into 1285.7 μl distilled water. 71 μl Starting Reaction Mixture (3 mL CoFactor Mixture; 1.2 mL Glucose-6-phosphate dehydrogenase solution) is added to 5 of the 6 test reactions and to the positive control. 71 μl 100 mM MgCl₂ is added to the sixth test reaction, which is used as a negative control. At each time point (0, 1, 3, 5, and 10 minutes), 75 μl of each reaction mix is pipetted into a well of a 96-well deep-well plate containing 75 μl ice-cold acetonitrile. Samples are vortexed and centrifuged 10 minutes at 3500 rpm (Sorval T 6000D centrifuge, HIOOOB rotor). 75 μl of supernatant from each reaction is transfened to a well of a 96-well plate containing 150 μl of a 0.5 μM solution of a compound with a known LCMS profile (internal standard) per well. LCMS analysis of each sample is carried out and the amount of unmetabolized test compound is measured as AUC, compound concentration vs. time is plotted, and the a value ofthe test compound is extrapolated.

Prefened compounds ofthe present invention exhibit in vitro t ₂ values of greater than 10 minutes and less than 4 hours, preferably between 30 minutes and 1 hour, in human liver microsomes. EXAMPLE 8 MDCK Toxicity Assay This Example illustrates the evaluation of compound toxicity using a Madin Darby canine kidney (MDCK) cell cytotoxicity assay. 1 μL of test compound is added to each well of a clear bottom 96-well plate

(PACKARD, Meriden, CT) to give final concentration of compound in the assay of 10 micromolar, 100 micromolar or 200 micromolar. Solvent without test compound is added to control wells.

MDCK cells, ATCC no. CCL-34 (American Type Culture Collection, Manassas, VA), are maintained in sterile conditions following the instructions in the ATCC production information sheet. Confluent MDCK cells are trypsinized, harvested, and diluted to a concentration of 0.1 x 10⁶ cells/ml with warm (37°C) medium (VITACELL Minimum Essential Medium Eagle, ATCC catalog # 30-2003). 100 μL of diluted cells is added to each well, except for five standard curve control wells that contain 100 μL of warm medium without cells. The plate is then incubated at 37°C under 95% O₂, 5% C0₂ for 2 hours with constant shaking. After incubation, 50 μL of mammalian cell lysis solution is added per well, the wells are covered with PACKARD TOPSEAL stickers, and plates are shaken at approximately 700 rpm on a suitable shaker for 2 minutes.

Compounds causing toxicity will decrease ATP production, relative to untreated cells. The PACKARD, (Meriden, CT) ATP-LITE-M Luminescent ATP detection kit, product no. 6016941, is generally used according to the manufacturer's instractions to measure ATP production in treated and untreated MDCK cells. PACKARD ATP LITE- M reagents are allowed to equilibrate to room temperature. Once equilibrated, the lyophilized substrate solution is reconstituted in 5.5 mis of substrate buffer solution (from kit). Lyophilized ATP standard solution is reconstituted in deionized water to give a 10 mM stock. For the five control wells, 10 μL of serially diluted PACKARD standard is added to each ofthe standard curve control wells to yield a final concentration in each subsequent well of 200 nM, 100 nM, 50 nM, 25 nM and 12.5 nM. PACKARD substrate solution (50 μL) is added to all wells, which are then covered, and the plates are shaken at approximately 700 rpm on a suitable shaker for 2 minutes. A white PACKARD sticker is attached to the bottom of each plate and samples are dark adapted by wrapping plates in foil and placing in the dark for 10 minutes. Luminescence is then measured at 22°C using a luminescence counter (e.g., PACKARD TOPCOUNT Microplate Scintillation and Luminescence Counter or TECAN SPECTRAFLUOR PLUS), and ATP levels calculated firom the standard curve. ATP levels in cells treated with test compound(s) are compared to the levels determined for untreated cells. Cells treated with 10 μM of a prefened test compound exhibit ATP levels that are at least 80%, preferably at least 90%, ofthe untreated cells. When a 100 μM concentration ofthe test compound is used, cells treated with prefened test compounds exhibit ATP levels that are at least 50%, preferably at least 80%, ofthe ATP levels detected in untreated cells.

EXAMPLE 9 Dorsal Root Ganglion Cell Assay This Example illustrates a representative dorsal root ganglian cell assay for evaluating VRl antagonist activity of a compound.

DRG are dissected from neonatal rats, dissociated and cultured using standard methods (Aguayo and White (1992) Brain Research 570:61-61). After 48 hour incubation, cells are washed once and incubated for 30-60 minutes with the calcium sensitive dye Fluo 4 AM (2.5-10 ug/ml; TefLabs, Austin, TX). Cells are then washed once, and various concentrations of compound is added to the cells. Addition of capsaicin to the cells results in a VRl -dependent increase in intracellular calcium levels which is monitored by a change in Fluo-4 fluorescence with a fluorometer. Data are collected for 60-180 seconds to determine the maximum fluorescent signal. Fluorescent signal is then plotted as a function of compound concentration to identify the concentration required to achieve a 50% inhibition ofthe capsaicin-activated response, or IC₅₀. Antagonists of the capsaicin receptor preferably have an IC₅₀ below 1 micromolar, 100 nanomolar, 10 nanomolar or 1 nanomolar.

EXAMPLE 10

Animal Models for Determining Pain Relief This Example illustrates representative methods for assessing the degree of pain relief provided by a compound.

A. Pain Relief Testing The following methods may be used to assess pain relief.

MECHANICAL ALLODYNIA

Mechanical allodynia (an abnormal response to an imiocuous stimulus) is assessed essentially as described by Chaplan et al. (1994) J. Neurosci. Methods 53:55-63 and Tal and Eliav (1998) Pain 64(3):511-518. A series of von Frey filaments of varying rigidity (typically 8-14 filaments in a series) are applied to the plantar surface ofthe hind paw with just enough force to bend the filament. The filaments are held in this position for no more than three seconds or until a positive allodynic response is displayed by the rat. A positive allodynic response consists of lifting the affected paw followed immediately by licking or shaking of the paw. The order and frequency with which the individual filaments are applied are determined by using Dixon up-down method. Testing is initiated with the middle hair of the series with subsequent filaments being applied in consecutive fashion, ascending or descending, depending on whether a negative or positive response, respectively, is obtained with the initial filament.

Compounds are effective in reversing or preventing mechanical allodynia-like symptoms if rats treated with such compounds require stimulation with a Von Frey filament of higher rigidity strength to provoke a positive allodynic response as compared to control untreated or vehicle treated rats. Alternatively, or in addition, testing of an animal in chronic pain may be done before and after compound administration. In such an assay, an effective compound results in an increase in the rigidity of the filament needed to induce a response after treatment, as compared to the filament that induces a response before treatment or in an animal that is also in chronic pain but is left untreated or is treated with vehicle. Test compounds are administered before or after onset of pain. When a test compound is administered after pain onset, testing is performed 10 minutes to three hours after administration.

MECHANICAL HYPERALGESIA

Mechanical hyperalgesia (an exaggerated response to painful stimulus) is tested essentially as described by Koch et al. (1996) Analgesia 2(3):157-164. Rats are placed in individual compartments of a cage with a wanned, perforated metal floor. Hind paw withdrawal duration (i.e., the amount of time for which the animal holds its paw up before placing it back on the floor) is measured after a mild pinprick to the plantar surface of either hind paw.

Compounds produce a reduction in mechanical hyperalgesia if there is a statistically significant decrease in the duration of hindpaw withdrawal. Test compound may be administered before or after onset of pain. For compounds administered after pain onset, testing is performed 10 minutes to three hours after administration. THERMAL HYPERALGESIA

Thermal hyperalgesia (an exaggerated response to noxious thermal stimulus) is measured essentially as described by Hargreaves et al. (1988) Pain. 32(l):77-88. Briefly, a constant radiant heat source is applied the animals' plantar surface of either hind paw. The time to withdrawal (i.e., the amount of time that heat is applied before the animal moves its paw), otherwise described as thermal threshold or latency, determines the animal's hind paw sensitivity to heat.

Compounds produce a reduction in thermal hyperalgesia if there is a statistically significant increase in the time to hindpaw withdrawal (i.e., the thermal threshold to response or latency is increased). Test compound may be administered before or after onset of pain. For compounds administered after pain onset, testing is performed 10 minutes to three hours after administration.

B. Pain Models

Pain may be induced using any of the following methods, to allow testing of analgesic efficacy of a compound, hi general, compounds provided herein result in a statistically significant reduction in pain as determined by at least one of the previously described testing methods, using male SD rats and at least one ofthe following models.

ACUTE INFLAMMATORY PAIN MODEL

Acute inflammatory pain is induced using the canageenan model essentially as described by Field et al. (1997) Br. J. Pharmacol. 121(8):1513-1522. 100-200 μl of 1- 2% canageenan solution is injected into the rats' hind paw. Three to four hours following injection, the animals' sensitivity to thermal and mechanical stimuli is tested using the methods described above. A test compound (0.01 to 50 mg/kg) is administered to the animal, prior to testing, or prior to injection of canageenan. The compound can be administered orally or through any parenteral route, or topically on the paw. Compounds that relieve pain in this model result in a statistically significant reduction in mechanical allodynia and/or thermal hyperalgesia.

CHRONIC INFLAMMATORY PAIN MODEL

Chronic inflammatory pain is induced using one ofthe following protocols: 1. Essentially as described by Bertorelli et al. (1999) Br. J. Pharmacol.

128(6): 1252-1258, and Stein et al. (1998) Pharmacol. Biochem. Behav. 31(2):455-51, 200 μl Complete Freund's Adjuvant (0.1 mg heat killed and dried M. Tuberculosis) is injected to the rats' hind paw: 100 μl into the dorsal surface and 100 μl into the plantar surface. 2. Essentially as described by Abbadie et al. (1994) J Neurosci. 14(10):5865- 5871 rats are injected with 150 μl of CFA (1.5 mg) in the tibio-tarsal joint. Prior to injection with CFA in either protocol, an individual baseline sensitivity to mechanical and thermal stimulation of the animals' hind paws is obtained for each experimental animal.

Following injection of CFA, rats are tested for thermal hyperalgesia, mechanical allodynia and mechanical hyperalgesia as described above. To verify the development of symptoms, rats are tested on days 5, 6, and 7 following CFA injection. On day 7, animals are treated with a test compound, morphine or vehicle. An oral dose of morphine of 1-5 mg/kg is suitable as positive control. Typically, a dose of 0.01-50 mg/kg of test compound is used. Compounds can be administered as a single bolus prior to testing or once or twice or three times daily, for several days prior to testing. Drags are administered orally or through any parenteral route, or applied topically to the animal.

Results are expressed as Percent Maximum Potential Efficacy (MPE). 0% MPE is defined as analgesic effect of vehicle, 100% MPE is defined as an animal's return to pre-CFA baseline sensitivity. Compounds that relieve pain in this model result in a MPE of at least 30%.

CHRONIC NEUROPATHIC PAIN MODEL

Chronic neuropathic pain is induced using the chronic constriction injury (CCI) to the rat's sciatic nerve essentially as described by Bennett and Xie (1988) Pain 33:87- 107. Rats are anesthetized (e.g. with an intraperitoneal dose of 50-65 mg/kg pentobarbital with additional doses administered as needed). The lateral aspect of each hind limb is shaved and disinfected. Using aseptic technique, an incision is made on the lateral aspect of the hind limb at the mid thigh level. The biceps femoris is bluntly dissected and the sciatic nerve is exposed. On one hind limb of each animal, four loosely tied ligatures are made around the sciatic nerve approximately 1-2 mm apart. On the other side the sciatic nerve is not ligated and is not manipulated. The muscle is closed with continuous pattern and the skin is closed with wound clips or sutures. Rats are assessed for mechanical allodynia, mechanical hyperalgesia and thermal hyperalgesia as described above. Compounds that relieve pain in this model result in a statistically significant reduction in mechanical allodynia, mechanical hyperalgesia and/or thermal hyperalgesia when administered (0.01-50 mg/kg, orally, parenterally or topically) immediately prior to testing as a single bolus, or for several days: once or twice or three times daily prior to testing.

Claims (120)

What is claimed is:

1. A compound of the formula:

or a pharmaceutically acceptable salt thereof, wherein:

V, X, W, Y and Z are each independently N or CR_1} with the proviso that at least one of V and X is N; U is N or CR₂, with the proviso that if V and X are N, then U is CR ;

Ri is independently selected at each occunence from hydrogen, halogen, hydroxy, amino, C_- C_.alkyl, haloCi-C_.alkyl, -Csalkoxy, halo -Csalkoxy and mono- and di-(C_-

C₈alkyl)amino; R₂ is: (i) hydrogen, halogen, cyano or -COOH;

(ii) Ci-Csalkanoyl, C -C₈alkanone, or -C_. carbamate, each of which is unsubstituted or substituted with from 1 to 9 substituents independently selected from R_t; or

(iii) a group ofthe formula -R_-M-A-R_y, wherein: R_e is C₀-C₃alkyl;

M is a bond, N(R_), O, S, SO₂, (C=O)_pN(R_z), N(R_z)(C=O)_p, SO₂N(R_z), or N(R_z)SO₂, wherein p is 0 or 1; A is a bond or Cι-C₈alkyl, optionally substituted with from 1 to 3 substituents independently selected from R ; and R_y and R_z, if present, are:

(a) independently hydrogen, Cι-C₈alkyl, C₂-C₈alkenyl, C₂-C₈alkynyl, C₆-

C.oarylC.-Csalkyl, C₂-C₈alkyl ether, C_.-Csalkoxy, a 4- to 10- membered carbocycle or heterocycle, or joined to R_ to form a 4- to

10-membered carbocycle or heterocycle, wherein each R_y and R_z is independently unsubstituted or substituted with from 1 to 9 substituents independently selected from R ; or (b) joined to form a 4- to 10-membered carbocycle or heterocycle that is unsubstituted or substituted with from 1 to 9 substituents independently selected from R_b; Ar₂ is a 5- to 7-membered aromatic heterocycle, optionally substituted with from 1 to 3 substituents independently selected from groups ofthe formula LR_a;

Ari is a 5- to 10-membered aromatic carbocycle or heterocycle, optionally substituted with from 1 to 3 substituents independently selected from groups ofthe formula LR_a; L is independently selected at each occunence firom a bond, -O-, -C(=O)-, -OC(=O)-, -C(=O)O-, -O-C(=0)O-, -S(O)_m-, -NR_X-, -C(=O)NHR_x-, -NHR_xC(=O)-, -NR_xS(0)_m-, -S(O)_mNR_x- and -N[S(O)_mR_x]S(O)_m-; wherein m is independently selected at each occurrence firom 0, 1 and 2; and R_x is independently selected at each occunence from hydrogen and Ci-Csalkyl; R_a is independently selected at each occunence from: (i) hydrogen, halogen, cyano and nitro; and (ii) -Csalkyl, C₂-C₈alkenyl, C₂-C₈alkynyl, C₂-C₈alkyl ether, 3- to 10-membered heterocycles, mono- and di-(C₁-C₈alkyl)amino and (3- to 10-membered heterocycle)C₁- C₆alkyl, each of which is unsubstituted or substituted with from 1 to 9 substituents independently selected from R_b; and R_b is independently chosen at each occunence from hydroxy, halogen, amino, aminocarbonyl, amido, cyano, nitro, -Csalkyl, -Csalkoxy, Ci-C_.alkylthio, -Csalkyl ether, hydroxyCi-Csalkyl, haloC_.-Csalkyl, phenyl, pheny^C Csalkyl), mono-and di-(Ci- C₆alkyl)amino, (SO^CrC_.alkyl, 5- to 7-membered heterocycle and (5- to 7-membered heterocycle)(C₁-C₈alkyl).

2. A compound according to claim 1, wherein V is N.

3. A compound according to claim 1 , wherein X is N.

4. A compound according to claim 3, wherein V is N.

5. A compound according to claim 3, wherein U is N.

6. A compound according to claim 1, wherein W, Y and Z are each independently N or CH.

7. A compound according to 6, wherein W and Y are each CH and Z is N, or wherein W and Z are each CH and Y is N.

8. A compound according to claim 1, wherein Ar₂ is selected from pyridyl, pyridazinyl, pyrimidinyl, pyrazinyl, pynolyl, imidazolyl, pyrazolyl, oxazolyl, isoxazolyl, thiazolyl, isothiazolyl and thiadiazolyl, each of which is unsubstituted or substituted with 1 or 2 substituents selected from halogen, cyano, C_.-Cealkyl, haloCrCβalkyl, hydroxyCi-Cβalkyl, C C_όalkyl ether, CrCβalkanoyl, amino, mono- and di-(C₁-C₆alkyl)amino.

9. A compound according to claim 1, wherein Ar₂ is pyridyl, isoxazolyl, thiadiazolyl or pyrazolyl, each of which is unsubstituted or substituted with halogen, C_- C alkyl or halo -C₄alkyl.

10. A compound according to claim 1, wherein Ar₁ is phenyl or pyridyl, optionally substituted with halogen, -C_δalkyl, halo -C_.alkyl, Ci-C₆alkoxy or haloC_.- C₆alkoxy.

11. A compound according to claim 1, wherein U is CR₂.

12. A compound according to claim 11, wherein R is: (i) hydrogen or halogen; or (ii) Ci-Cβalkyl, -(CH₂)_nNH₂, -(CH₂)_nNH(C₁-C₈alkyl), -(CH₂)_nN(C₁-C₈alkyl)₂,

-(CH₂)_n(5- to 8-membered heterocycloalkyl), -(CH₂)_nOH or -(CH₂)_nO(C₁-C₈alkyl), each of which is unsubsituted or substituted with from 1 to 4 substituents independently chosen from halogen, cyano, hydroxy, amino, mono- and di-(C₁- C₆alkyl)amino, Ci-Cβalkyl, and haloCi-C₆alkyl.

13. A compound according to claim 12, wherein X and V are each N.

14. A compound according to claim 13, wherein Aii and Ar₂ are each pyridyl, substituted with 1 substituent independently chosen from halogen, Ci-C₄alkyl, C_- C haloalkyl, and C₁-C₄alkoxy.

15. A compound according to claim 1, wherein the compound is selected from:

[7-(3-fluoro-pyridin-2-yl)-quinazolin-4-yl]-(5-trifluoromethyl-pyridin-2-yl)-amine; [6- (propane-2-sulfonyl)-pyridin-3-yl]-[7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]- amine hydrochloride; (5-tert-butyl-isoxazol-3-yl)-(7-pyridin-2-yl-quinazolin-4-yl)-amine; (5- trifluoromethyl-pyridin-2-yl)-[7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-amine; (6- Chloro-pyridin-3-yl)-[7-(2-trifluoromethyl-phenyl)-quinazolin-4-yl]-amine; (6-Isobutyl- pyridin-3-yl)-[7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-amine; (6-tert-Butyl- pyridin-3-yl)-[2-methyl-7-(3-methyl-pyridin-2-yl)-pyrido[2,3-d]pyrimidin-4-yl]-amine; (6- 1rifluoromethyl-pyridin-3-yl)-[7-(3-trifluoromethyl-pyridin-2-yl)-pyrido[3,2-^pyrimidin-4- yl]-amine; (6-trifluoromethyl-pyridin-3-yl)-[7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4- yl]-amine; (7-pyridin-2-yl-quinazolin-4-yl)-(5-trifluoromethyl-pyridin-2-yl)-amine; [2- chloro-7-(2-trifluoromethyl-phenyl)-pyrido[2,3-- ]pyrimidin-4-yl]-(6-trifluoromethyl-pyridin- 3-yl)-amine; [2-Methyl-7-(3-trifluoromethyl-pyridin-2-yl)-pyrido[2,3-d]pyrimidin-4-yl]-(6- trifluoromethyl-pyridin-3-yl)-amine; [2-methyl-7-(3-trifluoromethyl-pyridin-2-yl)- quinazolin-4-yl]-(6-trifluoromethyl-pyridin-3-yl)-amine; [7-(2-trifluoromethyl-phenyl)- quinazolin-4-yl]- (5-trifluoromethyl-pyridin-2-yl)-amine; (6-tert-Butyl-pyridin-3-yl)-[7-(3- chloro-pyridin-2-yl)-2-(2,6-dimethyl-mo holin-4-ylmethyl)-pyrido[3,2-d]pyrimidin-4-yl]- amine (cis); (6-Trifluoromethyl-pyridin-3-yl)-[7-(3-trifluoromethyl-pyridin-2-yl)-2-(3,3,5- trimethyl-azepan-l-ylmethyl)-quinazolin-4-yl]-amine; (R,R)-(6-tert-Butyl-pyridin-3-yl)-[2- (2,6-dimethyl-morpholin-4-ylmethyl)-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]- amine; (R,R)-(6-tert-Butyl-pyridin-3-yl)-[7-(3-chloro-pyridin-2-yl)-2-(2,6-dimethyl- morpholin-4-ylmethyl)-quinazolin-4-yl]-amine; (R,R)-[2-(2,6-Dimethyl-morpholin-4- ylmethyl)-7-(3 -trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl] -(6-trifluoromethyl-pyridin-3 - yl)-amine; (R,R)-[7-(3-Chloro-pyridin-2-yl)-2-(2,6-dimethyl-morpholin-4-ylmethyl)- quinazolin-4-yl]-(6-trifluoromethyl-pyridin-3-yl)-amine; (R,R)-[7-(3-Chloro-pyridin-2-yl)-2- (2,6-dimethyl-morpholin-4-ylmethyl)-quinazolin-4-yl]-(6-isopropoxy-pyridin-3-yl)-amine; (S,S)-(6-tert-Butyl-ρyridin-3-yl)-[2-(2,6-dimethyl-morpholin-4-ylmethyl)-7-(3- trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-amine; (S,S)-(6-tert-Butyl-pyridin-3-yl)-[7-(3- chloro-pyridin-2-yl)-2-(2,6-dimethyl-morpholin-4-ylmethyl)-quinazolin-4-yl]-amine; (S,S)- [2-(2,6-Dimethyl-morpholin-4-ylmethyl)-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]- (6-trifluoromethyl-pyridin-3-yl)-amine; (S,S)-[7-(3-Chloro-pyridin-2-yl)-2-(2,6-dimethyl- moφholin-4-ylmethyl)-quinazolin-4-yl]-(6-trifluoromethyl-pyridin-3-yl)-amine; [2-(l,l- Dioxo-lλ⁶-t omorpholin-4-ylmethyl)-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(6- trifluoromethyl-pyridin-3-yl)-amine; [2-(l ,4-Dioxa-8-aza-spiro[4.5]dec-8-ylmethyl)-7-(3- 1xifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(6-trifluoromethyl-pyridin-3-yl)-amine; [2-(l- Methyl-3,4-dihydro-lH-isoquinolin-2-ylmethyl)-7-(3-trifluoromethyl-pyridin-2-yl)- quinazolin-4-yl]-(6-trifluoromethyl-pyridin-3-yl)-amine; [2-(2,6-Dimethyl-moφholin-4- ylmethyl)-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(5-trifluoromethyl-pyridin-2- yl)-amine; [2-(2,6-Dimethyl-morpholin-4-ylmethyl)-7-(3-trifluoromethyl-pyridin-2-yl)- quinazolin-4-yl]-(6-trifluoromethyl-pyridin-3-yl)-amine (cis); [2-(2,6-Dimethyl-morpholin-4- ylmethyl)-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(5-trifluoromethyl-pyridin-2- yl)-amine (cis); [2-(2,6-Dimethyl-morpholin-4-ylmethyl)-7-(3-trifluoromethyl-pyridin-2-yl)- pyrido[3,2-d]pyrimidin-4-yl]-(6-trifluoromethyl-pyridin-3-yl)-amine (cis); [2-(2-Ethyl- piperidin-l-ylmethyl)-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(6-trifluoromethyl- pyridin-3-yl)-amine; [2-(2-Methyl-piperidin-l-ylmethyl)-7-(3-trifluoromethyl-pyridin-2-yl)- quinazolin-4-yl]-(6-trifluoromethyl-pyridin-3-yl)-amine; [2-(3,3-Dimethyl-piperidin-l- ylmethyl)-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(6-trifluoromethyl-pyridin-3- yl)-amine; [2-(3,4-Dihydro-lH-isoquinolin-2-ylmethyl)-7-(3-trifluoromethyl-pyridin-2-yl)- qumazolin-4-yl]-(6-trifluoromethyl-pyridin-3-yl)-amine; [2-(3,5-Dimethyl-piperidin-l- ylmethyl)-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(6-trifluoromethyl-pyridin-3- yl)-amine; [2-(3,5-Dimethyl-piperidin-l-ylmethyl)-7-(3-trifluoromethyl-pyridin-2-yl)- quinazolin-4-yl]-(6-trifluoromethyl-pyridin-3-yl)-amine; [2-(3-Methyl-piperidin-l-ylmethyl)- 7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(6-trifluoromethyl-pyridm-3-yl)-amine; [2-(4-Methyl-piperazin- 1 -ylmethyl)-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(5- trifluoromethyl-pyridin-2-yl)-amine; [2-(4-Methyl-piperidin- 1 -ylmethyl)-7-(3- trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(6-trifluoromethyl-pyridin-3-yl)-amine; [2- (Benzylamino-methyl)-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(5- trifluoromethyl-pyridin-2-yl)-amine; [2-(Benzylamino-methyl)-7-(3-trifluoromethyl-pyridin- 2-yl)-quinazolin-4-yl]-(6-trifluoromethyl-pyridin-3-yl)-amine; [2-(Octahydro-quinolin-l- ylmethyl)-7-(3 -trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl] -(6-trifluoromethyl-pyridin-3 - yl)-amine; [2-[(2-Methoxy-benzylamino)-methyl]-7-(3-trifluoromethyl-pyridin-2-yl)- quinazolin-4-yl]-(6-trifluoromethyl-pyridin-3-yl)-amine; [2-[(4-Methoxy-benzylamino)- methyl]-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(6-trifluoromethyl-pyridin-3-yl)- amine; [2-[(Allyl-methyl-amino)-methyl]-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4- yl]-(6-trifluoromethyl-pyridin-3-yl)-amine; [2-[(Benzyl-cyclopropyl-amino)-methyl]-7-(3- trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(6-trifluoromethyl-pyridin-3-yl)-amine; [2- [(Benzyl-methyl-amino)-methyl]-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(6- trifluoromethyl-pyridin-3-yl)-amine; [2-[(Benzyl-methyl-amino)-methyl]-7-(3- trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(6-trifluoromethyl-pyridin-3-yl)-amine; [2- [(Butyl-ethyl-amino)-methyl]-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(6- trifluoromethyl-pyridin-3-yl)-amine; [2-[(Butyl-methyl-amino)-methyl]-7-(3-trifluoromethyl- pyridin-2-yl)-quinazolin-4-yl]-(6-trifluoromethyl-pyridin-3-yl)-amine; [2-[(Cyclohexyl-ethyl- amino)-methyl] -7-(3 -trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl] -(6-trifluoromethyl- pyridin-3-yl)-amine; [2-[(Cyclohexyl-methyl-amino)-methyl]-7-(3-trifluoromethyl-pyridin-2- yl)-quinazolin-4-yl]-(6-trifluoromethyl-pyridin-3-yl)-amine; [2-[(Cyclopropylmethyl-propyl- amino)-methyl]-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(6-trifluoromethyl- pyridin-3-yl)-amine; [2-[(Cyclopropylmethyl-propyl-amino)-methyl]-7-(3-trifluoromethyl- pyridin-2-yl)-quinazolin-4-yl]-(6-trifluoromethyl-pyridin-3-yl)-amine; [2-[(Ethyl-isopropyl- amino)-methyl] -7-(3 -trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl] -(6-trifluoromethyl- pyridin-3-yl)-amine; [2-[(Hexyl-methyl-amino)-methyl]-7-(3-trifluoromethyl-pyridin-2-yl)- quinazolin-4-yl]-(6-trifluoromethyl-pyridin-3-yl)-amine; [2-[(Indan-l-yl-methyl-amino)- methyl]-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(6-trifluoromethyl-pyridin-3-yl)- amine; [2-[(Isopropyl-methyl-amino)-methyl]-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin- 4-yl]-(6-trifluoromethyl-pyridin-3-yl)-amine; [2-[(Methyl-phenethyl-amino)-methyl]-7-(3- trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(6-trifluoromethyl-pyridin-3-yl)-amine; [2- [(Methyl-propyl-amino)-methyl]-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(6- trifluoromethyl-pyridin-3-yl)-amine; [2-[(Tetrahydro-thiopyran-4-ylamino)-methyl]-7-(3- frifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(6-trifluoromethyl-pyridin-3-yl)-amine; [2-[3- (2,6-Dimethyl-morpholin-4-yl)-propyl]-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]- (6-trifluoromethyl-pyridin-3-yl)-amine; [2- {[(2-Fluoro-benzyl)-methyl-amino] -methyl} -7-(3- trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(6-trifluoromethyl-pyridin-3-yl)-amine; [2- {[(3-Fluoro-benzyl)-methyl-amino]-methyl}-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4- yl]-(6-trifluoromethyl-pyridin-3-yl)-amine; [2-{[(Pyridin-2-ylmethyl)-amino]-methyl}-7-(3- 1rifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(6-trifluoromethyl-pyridin-3-yl)-amine; [2- {[Bis-(2-methoxy-ethyl)-amino]-methyl}-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4- yl]-(5-trifluoromethyl-pyridin-2-yl)-amine; [2- {[Bis-(2-methoxy-ethyl)-amino] -methyl} -7-(3- tnfluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(6-trifluoromethyl-pyridin-3-yl)-amine; [2-{[Ethyl-(2-methyl-allyl)-amino]-methyl}-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4- yl]-(6-trifluoromethyl-pyridm-3-yl)-amine; [2-{[Methyl-(l-phenyl-ethyl)-amino]-methyl}-7- (3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(6-trifluoromethyl-pyridin-3-yl)-amine; [2- {[Methyl-(l-phenyl-propyl)-amino]-methyl}-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4- yl]-(6-trifluoromethyl-pyridin-3-yl)-amine; [2-{[Methyl-(2-methyl-benzyl)-amino]-methyl}-7- (3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(6-trifluoromethyl-pyridin-3-yl)-amine; [2- A__epan-l-ylmethyl-7-(3-tnfluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(6-trifluoromethyl- pyridin-3 -yl)-amine; [2-Azocan- 1 -ylmethyl-7-(3 -trifluoromethyl-pyridin-2 -yl)-quinazolin-4- yl]-(6-trifluoromethyl-pyridin-3-yl)-amine; [2-Diallylaminomethyl-7-(3-trifluoromethyl- pyridin-2-yl)-quinazolin-4-yl] -(6-trifluoromethyl-pyridin-3 -yl)-amine; [2- Dibutylaminomethyl-7-(3-t_rifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(6-trifluoromethyl- pyridin-3 -yl)-amine; [2-Diethylaminomethyl-7-(3 -trifluoromethyl-pyridin-2-yl)-quinazolin-4- yl]-(6-trifluoromethyl-pyridin-3-yl)-amine; [2-Dihexylaminomethyl-7-(3-trifluoromethyl- pyridin-2-yl)-quinazolin-4-yl] -(6-trifluoromethyl-pyridin-3 -yl)-amine; [2- Dimethylaminomethyl-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(6-trifluoromethyl- pyridin-3 -yl)-amine; [2-Dipentylaminomethyl-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin- 4-yl]-(6-trifluoromethyl-pyridin-3-yl)-amine; [2-Dipropylaminomethyl-7-(3-trifluoromethyl- pyridin-2-yl)-quinazolin-4-yl]-(6-trifluoromethyl-pyridin-3-yl)-amine; [2-

Dipropylaminomethyl-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(6-trifluoromethyl- pyridin-3 -yl)-amine; [2-Imidazol- 1 -ylmethyl-7-(3 -trifluoromethyl-pyridin-2-yl)-quinazolin-4- yl]-(6-trifluoromethyl-pyridin-3-yl)-amine; [2-Morpholin-4-ylmethyl-7-(3-trifluoromethyl- pyridin-2-yl)-pyrido[3,2-d]pyrimidin-4-yl]-(6-trifluoromethyl-pyridin-3-yl)-amine; [2- Momholin-4-ylmethyl-7-(3-trifluoromethyl-pyridin-2-yl)-pyrido[3,2-d]pyrimidin-4-yl]-(6- trifluoromethyl-pyridin-2 -yl)-amine; [2-Moroholin-4-ylmethyl-7-(3-trifluoromethyl-pyridin-2- yl)-quinazolin-4-yl]-(5-trifluoromethyl-pyridin-2-yl)-amine; [2-Morpholin-4-ylmethyl-7-(3- trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-[4-(2,2,2-trifluoro-l-methyl-ethyl)-phenyl]- amine; [2-Mo holin-4-yhτιethyl-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(6- trifluoromethyl-pyridin-3-yl)-amine; [2-Piperidin-l-ylmethyl-7-(3-trifluoromethyl-pyridin-2- yl)-quinazolin-4-yl]-(6-trifluoromethyl-pyridin-3-yl)-amine; [2-Pynolidin-l-ylmethyl-7-(3- 1rifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(6-trifluoromethyl-pyridin-3-yl)-amine; [2- Thiomoφholin-4-ylmethyl-7- (3 -trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl] -(6- trifluoromethyl-pyridin-3-yl)-amine; [7-(3-Chloro-pyridin-2-yl)-2-(2,6-dimethyl-moφholin-4- ylmethyl)-quinazolin-4-yl]-(6-trifluoromethyl-pyridin-3-yl)-amine (cis); [7-(3-Chloro-pyridin- 2-yl)-2-(2,6-dimethyl-moφholin-4-ylmethyl)-pyrido[3,2-d]pyrimidin-4-yl]-(6-trifluoromethyl- pyridin-3 -yl)-amine (cis); {l-[7-(3-Trifluoromethyl-pyridin-2-yl)-4-(6-trifluoromethyl- pyridin-3 -ylamino)-quinazolin-2-ylmethyl] -piperidin-4-yl} -methanol; { 1 -[7-(3 -

Trifluoromethyl-pyridin-2-yl)-4-(6-trifluoromethyl-pyridin-3-ylamino)-quinazolin-2- ylmethyl]-piperidin-3-yl} -methanol; 1 -[7-(3-Trifluoromethyl-pyridin-2-yl)-4-(6- 1τifluoromethyl-pyridin-3-ylamino)-quinazolin-2-ylmethyl]-piperidin-4-ol; l-[7-(3- Trifluoromethyl-pyridin-2-yl)-4-(6-trifluoromethyl-pyridin-3-ylamino)-quinazolin-2- ylmethyl]-piperidin-3-ol; 1 -[7-(3-Trifluoromethyl-pyridin-2-yl)-4-(6-trifluoromethyl-pyridin- 3-ylamino)-quinazolin-2-ylmethyl]-piperidine-4-carboxylic acid amide; l-{4-[7-(3- Trifluoromethyl-pyridin-2-yl)-4-(6-trifluoromethyl-pyridin-3-ylamino)-quinazolin-2- ylmethyl]-piperazin-l-yl}-ethanone; 2-{l-[7-(3-Trifluoromethyl-pyridin-2-yl)-4-(6- trifluoromethyl-pyridin-3-ylamino)-quinazolin-2-ylmethyl]-piperidin-4-yl} -ethanol; 2- {4-[7- (3 -Trifluoromethyl-p yridin-2-yl)-4-(6-trifluoromethyl-pyridin-3 -ylamino)-quinazolin-2- ylmethyl]-piperazin-l-yl} -ethanol; (6-tert-Butyl-pyridin-3-yl)-[2-(2-methoxy-ethyl)-7-(3- trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-amine; (6-tert-Butyl-pyridin-3-yl)-[2- isobutoxymethyl-7-(3-methyl-pyridin-2-yl)-pyrido[2,3-d]pyrimidin-4-yl]-amine; (6-tert-Butyl- pyridin-3-yl)-[2-isobutoxyιnethyl-7-(3-trifluoromethyl-pyridin-2-yl)-pyrido[2,3-d]pyrimidin- 4-yl]-amine; (6-tert-Butyl-pyridin-3-yl)-[2-methoxymethyl-7-(3-methyl-pyridin-2-yl)- pyrido[2,3-d]pyrimidin-4-yl]-amine; (6-tert-Butyl-pyridin-3-yl)-[2-methoxymethyl-7-(3- trifluoromethyl-pyridiιι-2-yl)-quinazolin-4-yl]-amine; (6-tert-Butyl-pyridin-3-yl)-[2- methoxymethyl-7-(3-trifluoromethyl-pyridin-2-yl)-pyrido[2,3-d]pyrimidin-4-yl]-amine; (6- tert-Butyl-pyridin-3-yl)-[7-(3-chloro-pyridin-2-yl)-2-ethoxymethyl-quinazolin-4-yl]-amine; (6-tert-Butyl-pyridin-3-yl)-[7-(3-chloro-pyridin-2-yl)-2-isobutoxymethyl-pyrido[2,3- d]pyrimidin-4-yl] -amine; (6-tert-Butyl-pyridin-3-yl)-[7-(3-chloro-pyridin-2-yl)-2- methoxymethyl-quinazolin-4-yl]-amine; (6-tert-Butyl-pyridin-3-yl)-[7-(3-chloro-pyridin-2- yl)-2-methoxymethyl-pyrido[2,3-d]pyrimidin-4-yl]-amine; [2-(l-Methyl-piperidin-4- yloxymethyl)-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(6-trifluoromethyl-pyridin- 3-yl)-amine; [2-(2-Diethylamino-ethoxymethyl)-7-(3-trifluoromethyl-pyridin-2-yl)- quinazolin-4-yl]-(6-trifluoromethyl-pyridin-3-yl)-amine; [2-(2-Piperidin-l-yl-ethoxymethyl)- 7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(6-trifluoromethyl-pyridin-3-yl)-amine; [2- (3-Benzyloxy-propyl)-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(6-trifluoromethyl- pyridin-3 -yl)-amine; [2-(3 -Benzyloxy-propyl)-7-(3 -trifluoromethyl-pyridin-2-yl)-quinazolin- 4-yl]-(6-tert-butyl-pyridin-3-yl)-amine; [2-(Pyridin-3-ylmethoxymethyl)-7-(3-trifluoromethyl- pyridin-2-yl)-quinazolin-4-yl]-(6-trifluoromethyl-pyridin-3-yl)-amine; [2-(Pyridin-4- ylmethoxymethyl)-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(6-trifluoromethyl- pyridin-3 -yl)-amine; [2-(Tetrahydro-pyran-4-yloxymethyl)-7-(3-trifluoromethyl-pyridin-2-yl)- quinazolin-4-yl]-(6-trifluoromethyl-pyridin-3-yl)-amine; [2-Benzyloxymethyl-7-(3- trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(6-trifluoromethyl-pyridin-3-yl)-amine; [2- B enzyloxymethyl-7-(3 -trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl] -(5 -trifluoromethyl- pyridin-2-yl)-amine; [2-Isobutoxymethyl-7-(3-trifluoromethyl-pyridin-2-yl)-pyrido[2,3- d]pyrimidin-4-yl]-(6-trifluoromethyl-pyridin-3-yl)-amine; [2-Isopropoxymethyl-7-(3- trifluoromethyl-pyridm-2-yl)-quinazolin-4-yl]-(6-trifluoromethyl-pyridin-3-yl)-amine; [2- Methoxymethyl-7-(3-methyl-pyridin-2-yl)-pyrido[2,3-d]pyrimidin-4-yl]-(6-trifluoromethyl- pyridin-3 -yl)-amine; [2-Methoxymethyl-7-(3-trifluoromethyl-pyridin-2-yl)-pyrido[2,3- d]pyrimidin-4-yl] -(6-trifluoromethyl-pyridin-3 -yl)-amine; [2-Methoxymethyl-7-(3 - trifluoromethyl-pyridin-2 -yl)-pyrido[3,2-d]pyrimidin-4-yl]-(6-trifluoromethyl-pyridin-3-yl)- amine; [2-Methoxymethyl-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(6- trifluoromethyl-pyridin-3-yl)-amine; [2-Methoxymethyl-7-(3-trifluoromethyl-pyridin-2-yl)- quinazolin-4-yl]-(5-trifluoromethyl-pyridin-2-yl)-amine; [7-(3-Chloro-pyridin-2-yl)-2- ethoxymethyl-pyrido[2,3-d]pyrimidin-4-yl]-(6-trifluoromethyl-pyridin-3-yl)-amine; [7-(3- Chloro-pyridin-2-yl)-2-isobutoxymethyl-pyrido[2,3-d]pyrimidin-4-yl]-(6-trifluoromethyl- pyridin-3 -yl)-amine; [7-(3 -Trifluoromethyl-pyridin-2-yl)-4-(6-trifluoromethyl-pyridin-3 - ylamino)-quinazolin-2-yl]-methanol; and 3-[7-(3-Trifluoromethyl-pyridin-2-yl)-4-(6- trifluoromethyl-pyridin-3-ylamino)-quinazolin-2-yl]-propan-l-ol.

16. A compound ofthe fonnula

or a pharmaceutically acceptable salt thereof, wherein:

V, X, W, Y and Z are each independently N or CR_l5 with the proviso that at least one of V and X is N; U is N or CR₂, with the proviso that if V and X are N, then U is CR₂;

Ri is independently selected at each occunence from hydrogen, halogen, hydroxy, amino, C_- C₈alkyl, haloCi-C₈alkyl, Ci-C₈alkoxy, haloCi-C₈alkoxy and mono- and di-(d- C₈alkyl)amino; R₂ is: (i) hydrogen, halogen, cyano or -COOH;

(ii) Ci-C₈alkanoyl, C₂-C₈alkanone, or Ci-C₈carbamate, each of which is unsubstituted or substituted with from 1 to 9 substituents independently selected from R ; or (iii) a group ofthe fonnula -R_c-M-A-R_y, wherein: R_e is Co-C₃alkyl;

M is a bond, N(R_), O, S, SO₂, (C=O)_pN(R_z), N(R_z)(C=O)_p, SO₂N(R₂), or

N(R_z)SO₂, wherein p is 0 or 1; A is a bond or C_.-C_.alkyl, optionally substituted with from 1 to 3 substituents independently selected from R_b; and R_y and R_z are:

(a) independently hydrogen, Ci-C₈alkyl, C₂-C₈alkenyl, C₂-C₈alkynyl, C₆- C₁₀arylCι-C₈alkyl, C₂-C₈alkyl ether, Ci-C₈alkoxy, a 4- to 10- membered carbocycle or heterocycle, or joined to R_e to form a 4- to 10-membered carbocycle or heterocycle, wherein each R_y and R_z is independently unsubstituted or substituted with from 1 to 9 substituents independently selected from R ; or

(b) joined to form a 4- to 10-membered carbocycle or heterocycle that is unsubstituted or substituted with from 1 to 9 substituents independently selected from R ; Ari and Ar are independently chosen from phenyl and 5- and 6-membered aromatic heterocycles, optionally substituted with from 1 to 3 substituents independently selected from groups ofthe formula LR_a; L is independently selected at each occunence from a bond, -O-, -C(=O)-, -OC(=O)-, -C(=0)O-, -O-C(=0)0-, -S(O)_m-, -NR_X-, -C(=O)NHR_x-, -NHR_xC(=O)-, -NR_xS(O)_m-, -S(O)_mNR_x- and -N[S(O)_mR ]S(O)_m-; wherein m is independently selected at each occurrence from 0, 1 and 2; and R_x is independently selected at each occunence from hydrogen and Ci-C₈alkyl; R_a is independently selected at each occunence from: (i) hydrogen, halogen, cyano and nitro; and

(ii) Cι-C₈alkyl, C₂-C₈alkenyl, C₂-C₈alkynyl, C₂-C₈alkyl ether, 3- to 10-membered heterocycles, mono- and di-(Ci-C₈alkyl)amino and (3- to 10-membered heterocycle)Ci- C₆alkyl, each of which is unsubstituted or substituted with from 1 to 9 substituents independently selected from R ;

R_b is independently chosen at each occunence from hydroxy, halogen, amino, aminocarbonyl, amido, cyano, nitro, Ci-C₈alkyl, Ci-C₈alkoxy, Ci-C₈alkylthio, Cι-C₈alkyl ether, hydroxyCi-C_.alkyl, haloCι-C₈alkyl, phenyl, phenyl(Ci-C_.alkyl), mono-and di-(d- C₆alkyl)amino, (SO₂)Cι-C₈alkyl, 5- to 7-membered heterocycle and (5- to 7-membered heterocycle)(Cι-C₈alkyl); and

R is Ci-C₈alkyl, C₂-C₈alkenyl, C₂-C₈alkynyl, mono- or di(Ci-C₈alkyl)amino or a 3- to 10- membered heterocycle, each of which is optionally substituted with from 1 to 5 substituents independently selected from hydroxy, halogen, Ci-C₆alkyl, Ci-C₈alkoxy, C₂- C_.alkyl ether, haloCi-C_.alkyl and haloCι-C₈alkoxy.

17. A compound according to claim 16, wherein V is N.

18. A compound according to claim 16, wherein X is N.

19. A compound according to claim 18, wherein V is N.

20. A compound according to claim 18, wherein U is N.

21. A compound according to claim 16, wherein W, Y and Z are each independently N or CH.

22. A compound according to 21, wherein W and Y are each CH and Z is N, or wherein W and Z are each CH and Y is N.

23. A compound according to claim 16, wherein Ar₂ is phenyl or pyridyl, each of which is optionally substituted with 1 or 2 substituents selected from halogen, cyano, C_- C₆alkyl and halod-C_όalkyl.

24. A compound according to claim 16, wherein Ar₂ is phenyl, optionally substituted with halogen, Cι-C₄alkyl or haloCi-C alkyl.

25. A compound according to claim 16, wherein Ari is phenyl or pyridyl, optionally substituted with halogen, -C_.alkyl, haloCi-C_.alkyl, Cι-C₆alkoxy or haloCi-

C₆alkoxy.

26. A compound according to claim 16, wherein U is CR .

27. A compound according to claim 26, wherein R₂ is

(i) hydrogen or halogen; or

(ii) C.-C₆alkyl, -(CH₂)_nNH₂, -(CH₂)_nNH(C₁-C₈alkyl), -(CH₂)_nN(C_.-C₈alkyl)₂,

-(CH₂)_n(5- to 8-membered heterocycloalkyl), -(CH₂)„OH or -(CH₂)_nO(Cι-C₈alkyl), each of which is unsubsituted or substituted with from 1 to 4 substituents independently chosen from halogen, cyano, hydroxy, amino, mono- and di-(Cι-

C₆alkyl)amino, Ci-C_όalkyl and haloCi-C₆alkyl.

28. A compound according to claim 27, wherein X and V are each N.

29. A compound according to claim 16, wherein R₇ comprises a nitrogen atom directly bonded to the SO₂.

30. A compound according to claim 29, wherein R₇ is amino, mono-or di(d- C₆alkyl)amino, moφholinyl, piperidinyl, piperazinyl or pynolidinyl, each of which is unsubstituted or substituted with from 1 to 3 substituents independently chosen from halogen, Cι-C₆alkyl and haloCi-C_.alkyl.

31. A compound according to claim 16, wherein R is C_-C₆alkyL haloCi- alkyl, moφholinyl, piperidinyl, piperazinyl or pynolidinyl, optionally substituted with from 1 to 5 substituents independently selected from Ci-C_δalkyl, Ci-C₆alkoxy, Ci-C₆alkyl ether, halod- C₆alkyl and haloCi-C₆alkoxy.

32. A compound according to claim 31, wherein Ari and Ar₂ are each pyridyl, substituted with 1 substituent independently chosen firom halogen, Ci-C₄alkyl, Ci- C₄haloalkyl, and Ci-C alkoxy.

33. A compound according to claim 16, wherein the compound is selected from:

[6-(propane-2-sulfonyl)-pyridin-3-yl]-[7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]- amine hydrochloride; (l-{4-[7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-ylamino]- benzenesulfonyl}-pynolidin-2-yl)-methanol (chiral); (l-{4-[7-(3-trifluoromethyl-pyridin-2- yl)-quinazolin-4-ylamino]-benzenesulfonyl} -pynolidin-2-yl)-methanol (chiral); (4- Methanesulfonyl-phenyl)-[2-methyl-7-(3-trifluoromethyl-pyridin-2-yl)-pyrido[2,3- d]pyrimidin-4-yl] -amine; (4-Methanesulfonyl-phenyl)-[7-(3-trifluoromethyl-pyridin-2-yl)- quinazolin-4-yl] -amine; (4-tert-Butyl-3-vinyl-phenyl)-[7-(3-trifluoromethyl-pyridin-2-yl)- pyrido[3,2-d]pyrimidin-4-yl]-amine; (4-trifluoromethanesulfonyl-phenyl)-[7-(3- trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-amine; (4-trifluoromethanesulfonyl-phenyl)- [7-(3-trifluoromethyl-pyridin-2-yl)-pyrido[3,2-d]pyrimidin4-yl]-amine; [2-Methyl-7-(3- trifluoromethyl-pyridin-2-yl)-pyrido[2,3-d]pyrimidin-4-yl]-(4-trifluoromethanesulfonyl- phenyl)-amine; [4-(2,5-dimethyl-pynolidine-l-sulfonyl)-phenyl]-[7-(3-trifluoromethyl- pyridin-2-yl)-quinazolin-4-yl]-amine; [4-(2,6-dimethyl-moφholine-4-sulfonyl)-phenyl]-[7- (3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-amine; [4-(2,6-Dimethyl-piperidine-l- sulfonyl)-phenyl]-[7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-amine (chiral); [4-(2- methoxymethyl-pynolidine-l-sulfonyl)-phenyl]-[7-(3-trifluoromethyl-pyridin-2-yl)- quinazolin-4-yl] -amine (chiral); [4-(2-methoxymethyl-pynolidine-l-sulfonyl)-phenyl]-[7-(3- trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-amine (chiral); [4-(2-methyl-piperdine-l- sulfonyl)-phenyl]-[7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-amine; [4-(2-methyl- pynolidine-l-sulfonyl)-phenyl]-[7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-amine; [4-(3-Dimethylamino-pynolidine-l-sulfonyl)-phenyl]-[7-(3-trifluoromethyl-pyridin-2-yl)- quinazolin-4-yl] -amine; [4-(3-Dimethylamino-pynolidine-l-sulfonyl)-phenyl]-[7-(3- trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-amine; [4-(4-Fluoro-piperidine-l-sulfonyl)- phenyl]-[7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-amine; [4-(4-Methyl-piperazine- l-sulfonyl)-phenyl]-[7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-amine; [4-

(moφholine-4-sulfonyl)-phenyl]-[7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-amine; [4-(moφholine-4-sulfonyl)-phenyl] -[7-(3 -trifluoromethyl-pyridin-2-yl)-pyrido [3 ,2- d]pyrimidin-4-yl] -amine; [4-(piperdine-l-sulfonyl)-phenyl]-[7-(3-trifluoromethyl-pyridin-2- yl)-quinazolin-4-yl] -amine; [4-(Propane-l-sulfonyl)-phenyl]-[7-(3-trifluoromethyl-pyridin-2- yl)-quinazolin-4-yl] -amine; [4-(pynolidine-l-sulfonyl)-phenyl]-[7-(3-trifluoromethyl- pyridin-2-yl)-quinazolin-4-yl]-amine; l-{4-[7-(3-Trifluoromethyl-pyridin-2-yl)-quinazolin-4- ylamino]-benzenesulfonyl}-pynolidin-3-ol (chiral); 4[4-(Azetidine-l-sulfonyl)-phenyl]-[7- (3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-amineN-(2-Hydroxy-l,l-dimethyl-ethyl)-4- [7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-ylamino]-benzenesulfonamide; N-(3- Chloro-propyl)-4-[7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-ylamino]- benzenesulfonamide; N,N-Bis-(2-methoxy-ethyl)-4-[7-(3-trifluoromethyl-pyridin-2-yl)- quinazolin-4-ylamino]-benzenesulfonamide; N,N-diisopropyl-4-[7-(3-trifluoromethyl- pyridin-2-yl)-quinazolin-4-ylamino]-benzenesulfonamide; N,N-dimethyl-4-[7-(3- trifluoromethyl-pyridin-2-yl)-quinazolin-4-ylamino]-benzenesulfonamide; N-Isopropyl-4-[7- (3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-ylamino]-benzenesulfonamide; N-tert-Butyl-4- [7-(3 -trifluoromethyl-pyridin-2-yl)-quinazolin-4-ylamino] -benzenesulfonamide; N-tert- Butyl-N-methyl-4-[7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-ylamino]- benzenesulfonamide; (R,R)-[2-(2,6-Dimethyl-moφholin-4-ylmethyl)-7-(3-trifluoromethyl- pyridin-2-yl)-quinazolin-4-yl]-[4-(propane-2-sulfonyl)-phenyl]-amine; (R,R)-[7-(3-Chloro- pyridin-2-yl)-2-(2,6-dimethyl-moφholin-4-ylmethyl)-quinazolin-4-yl]-[4-(propane-2- sulfonyl)-phenyl] -amine ; (S)-[7-(3-Chloro-pyridin-2-yl)-2-(2-methyl-moφholin-4- ylmethyl)-quinazolin-4-yl]-[4-(propane-2-sulfonyl)-phenyl]-amine; (S,S)-[2-(2,6-Dimethyl- moφholin-4-ylmethyl)-7-(3 -trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl] - [4-(propane-2- sulfonyl)-phenyl] -amine; (S,S)-[7-(3-Chloro-pyridin-2-yl)-2-(2,6-dimethyl-moφholin-4- ylmethyl)-quinazolin-4-yl]-[4-(propane-2-sulfonyl)-phenyl]-amine; [2-(2,6-Dimethyl- moφholin-4-ylmethyl)-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(4- trifluoromethanesulfonyl-phenyl)-amine; [2-(2,6-Dimethyl-moφholin-4-ylmethyl)-7-(3- trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(4-trifluoromethanesulfonyl-phenyl)-amine

(cis); [2-(2,6-Dimethyl-moφholin-4-ylmethyl)-7-(3-trifluoromethyl-pyridin-2-yl)-pyrido[3,2- d]pyrimidin-4-yl]-(4-methanesulfonyl-phenyl)-amine (cis); [2-(2,6-Dimethyl-moφholin-4- ylmethyl)-7-(3-trifluoromethyl-pyridin-2-yl)-pyrido[3,2-d]pyrimidin-4-yl]-(4- trifluoromethanesulfonyl-phenyl)-amine (cis); [2-(2,6-Dimethyl-moφholin-4-ylmethyl)-7-(3- trifluoromethyl-pyridin-2 -yl)-pyrido[3 ,2-d]pyrimidin-4-yl]-[4-(propane-l-sulfonyl)-phenyl]- amine (cis); [2-(2,6-Dimethyl-moφholin-4-ylmethyl)-7-(3-trifluoromethyl-pyridin-2-yl)- pyrido[3,2-d]pyrimidin-4-yl]-[4-(propane-2-sulfonyl)-phenyl]-amine (cis); [2-[(Ethyl-propyl- amino)-methyl]-7-(3-tirifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-[4-(moφholine-4- sulfonyl)-phenyl] -amine; [2-[(Isopropyl-methyl-amino)-methyl]-7-(3-trifluoromethyl- pyridin-2-yl)-qumazolin-4-yl]-[4-(moφholine-4-sulfonyl)-phenyl]-amine; [2-[(Methyl- propyl-amino)-methyl]-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-[4-(moφholine-4- sulfonyl)-phenyl]-amine; [2-Dimethylaminomethyl-7-(3-trifluoromethyl-pyridin-2-yl)- quinazolin-4-yl] - [4-(moφholine-4-sulfonyl)-phenyl] -amine; [2-Moφholin-4-ylmethyl-7-(3 - trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(4-trifluoromethanesulfonyl-phenyl)-amine; [7-(3-Chloro-pyridin-2-yl)-2-(2,6-dimethyl-moφholin-4-ylmethyl)-quinazolin-4-yl]-(4- trifluoromethanesulfonyl-phenyl)-amine (cis); [7-(3-Chloro-pyridin-2-yl)-2-(2,6-dimethyl- moφholin-4-ylnιethyl)-pyrido[3,2-d]pyrimidin-4-yl]-(4-trifluoromethanesulfonyl-phenyl)- amine (cis); [7-(3-Chloro-pyridin-2-yl)-2-(2,6-dimethyl-moφholin-4-ylmethyl)-pyrido[3,2- d]pyrimidin-4-yl]-[4-(moφholine-4-sulfonyl)-phenyl]-amine; (4-Methanesulfonyl-phenyl)- [2-methoxymethyl-7-(3 -trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl] -amine; (4- Methanesulfonyl-phenyl)-[2-me1hoxymethyl-7-(3-trifluoromethyl-pyridin-2-yl)-pyrido[2,3- d]pyrimidin-4-yl] -amine; [2-Benzyloxymethyl-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin- 4-yl] -(4-trifluoromethanesulfonyl-phenyl)-amine; [2-Benzyloxymethyl-7-(3 -trifluoromethyl- pyridin-2 -yl)-quinazolin-4-yl] -(4-methanesulfonyl-phenyl)-amine; [2-Benzyloxymethyl-7-(3 - trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(l-methanesulfonyl-2,3-dihydro-lH-indol-5- yl)-amine; [2-Cyclopentyloxymethyl-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-[4- (moφholine-4-sulfonyl)-phenyl]-amine; [2-Cyclopropylmethoxymethyl-7-(3- trifluoromethyl-pvridin-2-yl)-quinazolin-4-yl]-[4-(moφholine-4-sulfonyl)-phenyl]-amine; [2- Ethoxymethyl-7-(3-methyl-pyridin-2-yl)-pyrido[2,3-d]pyrimidin-4-yl]-(4- trifluoromethanesulfonyl-phenyl)-amine; [2-Ethoxymethyl-7-(3-trifluoromethyl-pyridin-2- yl)-quinazolin-4-yl]-[4-(moφholine-4-sulfonyl)-phenyl]-amine; [2-Isobutoxymethyl-7-(3- methyl-pyridin-2-yl)-pyrido[2,3-d]pyrimidin-4-yl]-(4-methanesulfonyl-phenyl)-amine; [2- Isobutoxymethyl-7-(3-trifluoromethyl-pyridin-2-yl)-pyrido[2,3-d]pyrimidin-4-yl]-(4- trifluoromethanesulfonyl-phenyl)-amine; [2-Isobutoxymethyl-7-(3-trifluoromethyl-pyridin-2- yl)-pyrido[2,3-d]pyrimidin-4-yl]-(4-methanesulfonyl-phenyl)-amine; [2-Isopropoxymethyl-7- (3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-[4-(moφholine-4-sulfonyl)-phenyl]-amine; [2-Methoxymethyl-7-(3-methyl-pyridin-2-yl)-pyrido[2,3-d]pyrimidin-4-yl]-(4- trifluoromethanesulfonyl-phenyl)-amine; [2-Methoxymethyl-7-(3-trifluoromethyl-pyridin-2- yl)-pyrido[2,3-d]pyrimidin-4-yl]-(4-trifluoromethanesulfonyl-phenyl)-amine; [2- Methoxymethyl-7-(3-1rifluoromethyl-pyridin-2-yl)-pyrido[2,3-d]pyrimidin-4-yl]-[4- (moφholine-4-sulfonyl)-phenyl]-amine; [2-Methoxymethyl-7-(3-trifluoromethyl-pyridin-2- yl)-quinazolin-4-yl]-(4-trifluoromethanesulfonyl-phenyl)-amine; [2-Methoxymethyl-7-(3- trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-[4-(moφholine-4-sulfonyl)-phenyl]-amine; [4- (Moφholine-4-sulfonyl)-phenyl]-[2-(tetrahydro-pyran-4-yloxymethyl)-7-(3-trifluoromethyl- pyridin-2-yl)-quinazolin-4-yl]-amine; [4-[4-(Piperidine-l-sulfonyl)-phenylamino]-7-(3- trifluoromethyl-pyridin-2-yl)-quinazolin-2-yl]-methanol; [4-[4-(Piperidine-l-sulfonyl)- phenylamino]-7-(3-trifluoromethyl-pyridin-2-yl)-pyrido[3,2-d]pyrimidin-2-yl]-methanol; [7- (3-Chloro-pyridin-2-yl)-2-ethoxymethyl-pyrido[2,3-d]pyrimidin-4-yl]-(4- trifluoromethanesulfonyl-phenyl)-amine; [7-(3-Chloro-pyridin-2-yl)-2-isobutoxymethyl- pyrido[2,3-d]pyrimidin-4-yl]-(4-trifluoromethanesulfonyl-phenyl)-amine; [7-(3-Chloro- pyridin-2-yl)-2-methoxymethyl-pyrido[3,2-d]pyrimidin-4-yl]-[4-(moφholine-4-sulfonyl)- phenyl]-amine; 4-[2-Benzyloxymethyl-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4- ylamino]-N-tert-butyl-benzenesulfonamide; N-tert-Butyl-4-[2-hydroxymethyl-7-(3- trifluoromethyl-pyridin-2-yl)-quinazolin-4-ylamino]-N-methyl-benzenesulfonamide; N-tert- Butyl-4-[2-hydroxymethyl-7-(3-trifluoromethyl-pyridin-2-yl)-pyrido[3,2-d]pyrimidin-4- ylamino]-N-methyl-benzenesulfonamide; and N-tert-Butyl-4-[2-methoxymethyl-7-(3- trifluoromethyl-pyridin-2-yl)-quinazolin-4-ylamino]-benzenesulfonamide.

34. A compound ofthe formula:

wherein:

V, X, W, Y and Z are each independently N or CR_l5 with the proviso that at least one of V and X is N; Ri is independently selected at each occunence from hydrogen, halogen, hydroxy, cyano, amino, Ci-C₈alkyl, haloCi-C₈alkyl, Ci-C₈alkoxy, haloCi-C₈alkoxy and mono-and di-(Ci- C₆alkyl)amino;

Ari and Ar₂ are independently selected from phenyl and 5- to 7-membered aromatic heterocycles, each of which is unsubstituted or substituted with from 1 to 3 substituents independently selected firom groups ofthe formula LR_a;

L is independently selected at each occunence from a bond, -0-, -C(=O)-, -OC(=O)-, -C(=0)0-, -0-C(=O)0-, -S(O)_m-, -NR_X-, -C(=O)NHR_x-, -NHR_xC(=O)-, -NR_xS(O)_m-, -S(O)_mNR_x- and -N[S(O)_mR_x]S(O)_m-; wherein m is independently selected at each occunence from 0, 1 and 2; and R_x is independently selected at each occurrence from hydrogen and Ci-C₈alkyl;

R_a is independently selected at each occunence firom: (i) hydrogen, halogen, cyano and nitro; and

(ii) Cι-C₈alkyl, C₂-C₈alkenyl, C -C₈alkynyl, C -C₈alkyl ether, 3- to 10-membered heterocycles, and (3- to 10-membered heterocycle)Ci-C₆alkyl, each of which is unsubstituted or substituted with firom 1 to 9 substituents independently selected from R_b;

R₃ and R₄ are: (i) each independently selected from:

(a) hydrogen;

(b) Ci-C₈alkyl, C₂-C₈alkenyl, C₂-C₈alkynyl, Ci-C₈alkoxy, C₃-C₈alkanone, C₂- C_.alkanoyl, C₂-C₈alkyl ether, C₆-CioarylC₀-C₈alkyl, 5- to 10-membered heterocycleC₀-C₈alkyl and -(SO₂)Ci-C₈alkyl, each of which is optionally substituted with from 1 to 9 substituents independently selected from R_b; and

(c) groups that are joined to an R₅ or R₆ to form a 4- to 10-membered heterocyclic group that is unsubstituted or substituted with from 1 to 6 substituents independently selected from R_b; or

(ii) joined to form, with the N to which they are bound, a 4- to 10-membered heterocyclic group that is unsubstituted or substituted with from 1 to 6 substituents independently selected from R , Ci-C₈alkanoyl, 4- to 7-membered heterocycloalkylC₀-C₄alkyl, and mono- and di-(Ci-C₆alkyl)aminoCi-C₆alkyl; R₅ and Re are, independently at each occunence: (i) each independently selected firom: (a) hydrogen or hydroxy;

(b) Cι-C₈alkyl, unsubstituted or substituted with 1 or 2 substituents independently selected from R_b; and

(c) groups that are joined to R₃ or R₄ to form a 4- to 10-membered heterocyclic group that is unsubstituted or substituted with from 1 to 6 substituents independently selected firom R_b;

(ii) taken together to form a keto group; or

(iii) joined to form a 3- to 7-membered carbocyclic or heterocyclic ring, unsubstituted or substituted with firom 1 to 4 substituents selected from R ; R_b is independently chosen at each occunence from hydroxy, halogen, amino, aminocarbonyl, amido, cyano, nitro, Ci-C₈alkyl, Ci-C₈alkoxy, Ci-C₈alkylthio, C -C₈alkyl ether, hydroxyCi-C₈alkyl, haloCi-C₈alkyl, phenyl, phenyl(Ci-C₈alkyl), mono-and di-(d-

C₆alkyl)amino, (SO₂)Ci-C₈alkyl, 5- to 7-membered heterocycle and (5- to 7-membered heterocycle)(Ci-C₈alkyl); and n is 1, 2 or 3.

35. A compound or salt according to claim 34, wherein V and X are both N.

36. A compound or salt according to claim 34, wherein W is N and Y and Z are

CH.

37. A compound or salt according to claim 34, wherein Y is N and W and Z are CH.

38. A compound or salt according to claim 34, wherein Z is N and W and Y are CH.

39. A compound or salt according to claim 34, wherein W, Y and Z are each CH.

40. A compound or salt according to claim 34, wherein Ari and Ar are independently selected from phenyl and 6-membered aromatic heterocycles, each of which is substituted with 0, 1 or 2 substituents.

41. A compound or salt according to claim 40, wherein:

(i) Ari is phenyl or pyridyl, each of which is unsubstituted or substituted with 1 or 2 substituents selected from halogen, hydroxy, cyano, amino, nitro, mono- and di-(d- C₆alkyl)amino, Ci-C₆alkyl, halod-C₆alkyl, d-C₆alkoxy and halod-C₆alkoxy; and

(ii) Ar₂ is phenyl or pyridyl, each of which is unsubstituted or substituted with 1 or 2 substituents independently selected from halogen, hydroxy, cyano, amino, mono- and di-(C₁-

C₆alkyl)amino, Ci-C₆alkyl, haloCι-C₆alkyl, d-C_.alkoxy, halod-C₆alkoxy, C₂-C₆alkyl ether,

Ci-C₆alkanoyl, -(SO₂)R₂, -NR_xS(O)_m-, and -N(S(O_m)₂; wherein m is 1 or 2, R_x is hydrogen or Ci-C₆alkyl, and R₂ is d-C₆alkyl, haloCi-C₆alkyl, amino, mono- or di-(Ci-C₆alkyl)amino or a 5- to 10-membered, N-linked heterocyclic group, each of which R₂ is optionally substituted with R .

42. A compound or salt according to claim 41, wherein:

(i) Ari is pyridyl, unsubstituted or substituted with halogen, Ci-C4alkyl or halod- C₄alkyl; and

(ii) Ar₂ is phenyl or pyridyl, each of which is unsubstituted or substituted with halogen, cyano, d-C₄alkyl, haloCi-C₄alkyl, C₂-C₄alkyl ether, d-C₄alkanoyl or -(SO₂)R_a, wherein R_a is Cι-C₄alkyl or halod-C₄alkyl.

43. A compound or salt according to claim 42, wherein:

(i) Ari is pyridin-2-yl, 3-methyl-pyridin-2-yl, 3-trifluoromethyl-pyridin-2-yl or 3-halo- pyridin-2-yl; and

(ii) Ar₂ is phenyl, 2-pyridyl or 3-pyridyl, each of which is substituted at the 4-position with trifluoromethanesulfonyl, propanesulfonyl, propane-2-sulfonyl, t-butyl, trifluoromethyl or 2,2,2-trifluoro-l -methyl-ethyl.

44. A compound or salt according to claim 34, wherein R₃ and R are each independently: (i) hydrogen; or

(ii) Ci-C₈alkyl, C₂-C₈alkenyl, phenylC₀-C₄alkyl, indanylCo-C₄alkyl, 5- to 6-membered heteroarylC₀-C alkyl, or 4- to 7-membered heterocycloalkylC₀-C₄alkyl, each of which is unsubstituted or substituted with from 1 to 4 substituents independently selected from hydroxy, halogen, amino, d-C₆alkyl, haloCι-C₆alkyl, Ci-C₆alkoxy and haloCi-C₆alkoxy.

45. A compound or salt according to claim 44, wherein R₃ and R₄ are each independently

(i) hydrogen; or

(ii) Cι-C₆alkyl, C₂-C₆alkenyl, 5- to 7-membered heterocycloC₀-C alkyl, C₂-C₆alkyl ether, indanyl, benzyl, 1-phenyl-ethyl, 1 -phenyl-propyl and 2-phenyl-ethyl, each of which is unsubstituted or substituted with from 1 to 3 substituents independently selected from hydroxy, halogen and d-C₄alkyl.

46. A compound or salt according to claim 44, wherein one of R₃ and R_i is pyridylCo-C₄alkyl, pyrimidylC₀-C₄alkyl, imidazolylCo-C _.alkyl or tetrazolylCo-C₄alkyl, each of which is substituted with 0, 1 or 2 substituents.

47. A compound or salt according to claim 34, wherein R₃ and t are joined to form a 5 to 10-membered heterocyclic group that is substituted with from 0 to 4 substituents.

48. A compound or salt according to claim 47, wherein the heterocyclic group is substituted with at least one substituent selected from hydroxy, halogen, d-C₄alkyl, halod- C₄alkyl, d-C₄alkoxy, halod-C₄alkoxy, d-C₄alkanoyl, and aminocarbonyl.

49. A compound or salt according to claim 47, wherein the heterocyclic group comprises an aromatic ring.

50. A compound or salt according to claim 49, wherein the heterocyclic group is

3,4-dihydro-lH-isoquinolin-2-yl, substituted with 0, 1 or 2 substituents.

51. A compound or salt according to claim 47, wherein the heterocyclic group is a 5- to 10-membered heterocycloalkyl, substituted with firom 0 to 4 substituents.

52. A compound or salt according to claim 51, wherein the heterocycloalkyl is piperadinyl, piperazinyl, pynolidinyl, azepanyl, azocinyl, decahydroquinolinyl or 1,4-dioxa- 8-aza-spiro[4.5]dec-8-yl, each of which is unsubstituted or substituted with from 1 to 4 substituents independently selected from halogen, hydroxy, d-C₄alkyl, d-C₄alkoxy, halod- C alkyl, haloCi-C₄alkoxy, Ci-C₄alkanoyl and Ci-C₄alkoxycarbonyl.

53. A compound or salt according to claim 51, wherein the heterocyclic group is moφholino, thiomoφholino or l,l-dioxo-tlιiomoφholin-4-yl, each of which is unsubstituted or substituted with from 1 to 4 substituents independently selected from halogen, hydroxy, Ci-dalkyl, Ci-C₄alkoxy, halod-C₄alkyl, halod-C alkoxy, d-C alkanoyl and C_- C alkoxycarbonyl.

54. A compound or salt according to claim 52 or 53, wherein the heterocyclic group is substituted with from 1 to 4 substituents independently selected from methyl and ethyl.

55. A compound or salt according to claim 34, wherein each R₅ and R₆ is independently selected from hydrogen and d-C₆alkyl.

56. A compound or salt according to claim 55, wherein each R₅ and R₆ is hydrogen.

57. A compound or salt according to claim 34, wherein n is 1.

58. A compound or salt according to claim 34, wherein: (i) V and X are N; (ii) Ari is pyridyl, unsubstituted or substituted with halogen, d-C₄alkyl or halod-

C₄alkyl;

(iii)Ar₂ is phenyl or pyridyl, unsubstituted or substituted with d-Qalkyl, haloCι-C₄alkyl or a group ofthe formula -(SO₂)R₂, wherein R₂ is Ci-C₄alkyl or haloCi-C₄alkyl;

(iv)R₃ and _t are each independently selected from d-C_.alkyl, C -C₆alkenyl, C₂-C₆alkyl ether, 5- to 10-membered heteroarylC₀-C₄alkyl, phenylC₀-C₄alkyl and indanyl, each of which is substituted with 0, 1 or 2 substituents independently selected from hydroxy, halogen, C_- C₄alkyl and haloCι-C₄alkyl; and (v) n is 1.

59. A compound or salt according to claim 34, wherein:

(i) V and X are N;

(ii) Ari is pyridyl, unsubstituted or substituted with halogen, d-C₄alkyl or haloC_.- dalkyl;

(iii)Ar₂ is phenyl or pyridyl, unsubstituted or substituted with d-C₄alkyl, halod-Qalkyl or a group ofthe formula -(SO₂)R₂, wherein R₂ is Cι-C₄alkyl or haloCi-C₄alkyl;

(iv)R₃ and i are joined to form a 5- to 10-membered heterocyclic group that is unsubstituted or substituted with firom 1 to 3 substituents; and (v) n is 1.

60. A compound according to claim 34, wherein the compound is selected from: [2-Pynolidin-l-ylmethyl-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(4-1rrifluoro methyl-phenyl)-amine; [2-(2,6-Dimethyl-moφholin-4-ylmethyl)-7-(2-trifluoromethyl- phenyl)-pyrido[4,3-d]pyrimidin-4-yl]-(4-trifluoromethyl-phenyl)-amine (cis); [2-Mθφholin- 4-ylmethyl-7(3-trifluoromethyl-pyridin-2-yl)-pyrido[3,2-a]pyrimidin-4-yl]-(4- trifluoromethyl-phenyl)-amine; [2-(2-Pynolidin- 1 -yl-ethyl)-7-(3 -trifluoromethyl-pyridiny-2- yl)-quinazolin-4-yl]-(4-trifluoromethyl-phenyl)-amine; l-moφholin-4-yl-3-[4-(4- trifluoromethyl-phenylamino)-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-2-yl]-propan-l- one, [2-(3-moφholin-4-yl-propyl)7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(4- trifluoromethyl-phenyl)-amine hydrochloride; 4-trifluoromethylphenyl-[2-(2,6- dimethylmoφhonli-4-ylmethyl)-7-(2-trifluoromethyl phenyl)-quinazolin-4-yl]-amine; [7-(3- Methyl-pyridin-2-yl)-2-pynOlidin- 1 -ylmethyl-pyrido [3 ,2-d]pyrimidin-4-yl] -(4- trifluoromethyl-phenyl)-amine; (1 - {3-[4-(4-Trifluoromethyl-phenylamino)-7-(3- trifluoromethyl-pyridin-2-yl)-quinazolin-2-yl] -propyl} -piperidin-4-yl)-methanol; (2,6-

Dimethyl-moφholin-4-yl)-[4-(4-trifluoromethyl-phenylamino)-7-(3-trifluoromethyl-pyridin- 2-yl)- quinazolin-2-yl]-methanone (cis); (4-Cyclopropyl-phenyl)-[2-(2,2-dimethyl- moφholin-4-ylmethyl)-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-amine; (4- Cyclopropyl-phenyl)-[2-(2,6-dimethyl-moφholin-4-ylmethyl)-7-(3-trifluoromethyl-pyridin- 2-yl)-quinazolin-4-yl] -amine (cis); (4-Isopropyl-phenyl)-[2-[(methyl-propyl-amino)-methyl]- 7-(3-methyl-pyridin-2-yl)-quinazolin-4-yl]-amine; (4-Isopropyl-phenyl)-[7-(3-methyl- pyridin-2-yl)-2-moφholin-4-yhnethyl-quinazolin-4-yl]-amine; (4-Isopropyl-phenyl)-[7-(3- methyl-pyridin-2-yl)-2-thiomoφholin-4-ylmethyl-quinazolin-4-yl]-amine; (4-_^,ec-Butyl- phenyl)-[7-(3-chloro-pyridin-2-yl)-2-(2,6-dimethyl-moφholin-4-ylmethyl)-quinazolin-4-yl]- amine (cis); (4-tert-Butyl-phenyl)-[2-(l,l-dioxo-lλ⁶-isothiazolidin-2-ylmethyl)-7-(3- trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-amine; (4-tert-Butyl-phenyl)-[2-(2,6-dimethyl- moφholin-4-ylmethyl)-7-(2-trifluoromethyl-phenyl)-pyrido[4,3-d]pyrimidin-4-yl]-amine (cis); (4-tert-Butyl-phenyl)-[2-(2,6-dimethyl-moφholin-4-ylmethyl)-7-(3-trifluoromethyl- pyridin-2-yl)-pyrido[2,3-d]pyrimidin-4-yl]-amine (cis); (4-tert-Butyl-phenyl)-[2-(2,6- dimethyl-moφholin-4-ylmethyl)-7-(3-trifluoromethyl-pyridin-2-yl)-pyrido[3,2-d]pyrimidin- 4-yl] -amine (cis); (4-tert-Butyl-phenyl)-[2-(2,6-dimethyl-moφholin-4-ylmethyl)-7-(3- methyl-pyridin-2-yl)-quinazolin-4-yl]-amine (cis); (4-tert-Butyl-phenyl)-[2-(2,6-dimethyl- moφholin-4-ylmethyl)-7-(6-methyl-pyridin-2-yl)-quinazolin-4-yl]-amine (cis); (4-tert-Butyl- phenyl)-[2-(2,6-dimethyl-moφholin-4-yhnethyl)-7-pyridin-2-yl-quinazolin-4-yl]-amine (cis); (4-tert-Butyl-phenyl)-[2-(2,6-dimethyl-moφholin-4-ylmethyl)-7-pyridin-4-yl-quinazolin-4- yl]-amine (cis); (4-tert-Butyl-phenyl)-[2-(2,6-dimethyl-moφholin-4-ylmethyl)-7-pyridin-3- yl-quinazolin-4-yl] -amine (cis); (4-tert-Butyl-phenyl)-[2-(2,6-dimethyl-moφholin-4- ylmethyl)-7-pyrimidin-5-yl-quinazolin-4-yl]-amine (cis); (4-tert-Butyl-phenyl)-[2-piperidin- l-ylmethyl-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-amine; (4-tert-Butyl-phenyl)- [2-pynolidin-l-ylmethyl-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-amine; (4-tert- Butyl-phenyl)-[7-(2,4-dimethoxy-pyrimidin-5-yl)-2-(2,6-dimethyl-moφholin-4-ylmethyl)- quinazolin-4-yl] -amine (cis); (4-tert-Butyl-phenyl)-[7-(3-chloro-pyridin-2-yl)-2-(2,6- dimethyl-moφholin-4-ylmethyl)-pyrido[3,2-d]pyrimidin-4-yl]-amine (cis); (4-

Trifluoromethyl-phenyl)-[7-(3-trifluoromethyl-pyridin-2-yl)-2-(3,3,5-trimethyl-azepan-l- ylmethyl)-quinazolin-4-yl]-amine; (4-Trifluoromethyl-phenyl)-{7-(3-trifluoromethyl-pyridin- 2-yl)-2-[2-(3,3,5-trimethyl-azepan-l-yl)-ethyl]-quinazolin-4-yl}-amine; (6-tert-Butyl-pyridin- 3-yl)-[7-(3-chloro-pyridin-2-yl)-2-(2,6-dimethyl-moφholin-4-ylmethyl)-pyrido[3,2- d]pyrimidin-4-yl] -amine (cis); (6-Trifluoromethyl-pyridin-3-yl)-[7-(3-trifluoromethyl- pyridin-2-yl)-2-(3,3,5-trimethyl-azepan-l-ylmethyl)-quinazolin-4-yl]-amine; (R)-(4- Isopropyl-phenyl)-[2-(2-methyl-moφholin-4-ylmethyl)-7-(3-trifluoromethyl-pyridin-2-yl)- quinazolin-4-yl] -amine; (R)-(4-tert-Butyl-phenyl)- [2-(2-methyl-moφholin-4-ylmethyl)-7-(3 - trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-amine; (R)-(4-tert-Butyl-phenyl)-[7-(3-chloro- pyridin-2-yl)-2-(2-methyl-moφholin-4-ylmethyl)-quinazolin-4-yl]-amine; (R)-[2-(2-Methyl- moφholin-4-ylmethyl)-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(4- trifluoromethyl-phenyl)-amine; (R)-[7-(3-Chloro-pyridin-2-yl)-2-(2-methyl-moφholin-4- ylmethyl)-quinazolin-4-yl]-(4-isopropyl-phenyl)-amine; (R)-[7-(3-Chloro-pyridin-2-yl)-2-(2- methyl-moφholin-4-ylmethyl)-quinazolin-4-yl]-(4-trifluoromethyl-phenyl)-amine; (R,R)-(4- Chloro-phenyl)-[2-(2,6-dimethyl-moφholin-4-ylmethyl)-7-(3-trifluoromethyl-pyridin-2-yl)- quinazolin-4-yl] -amine; (R,R)-(4-Chloro-phenyl)-[7-(3-chloro-pyridin-2-yl)-2-(2,6-dimethyl- moφholin-4-ylmethyl)-quinazolin-4-yl]-amine; (R,R)-(4-Ethyl-phenyl)-[7-(3-chloro-pyridin- 2-yl)-2-(2,6-dimethyl-moφholin-4-ylmethyl)-quinazolin-4-yl]-amine; (R,R)-(4-Fluoro- phenyl)-[7-(3-chloro-pyridin-2-yl)-2-(2,6-dimethyl-moφholin-4-ylmethyl)-quinazolin-4-yl]- amine; (R,R)-(4-Isopropyl-phenyl)-[7-(3-chloro-pyridin-2-yl)-2-(2,6-dimethyl-moφholin-4- ylmethyl)-quinazolin-4-yl]-amine; (R,R)-(4-tert-Butyl-phenyl)-[7-(3-chloro-pyridin-2-yl)-2- (2,6-dimethyl-moφholin-4-yhnethyl)-quinazolin-4-yl]-amine; (R,R)-(6-tert-Butyl-pyridin-3- yl)-[2-(2,6-dimethyl-moφholin-4-ylmethyl)-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4- yl]-amine; (R,R)-(6-tert-Butyl-pyridin-3-yl)-[7-(3-chloro-ρyridin-2-yl)-2-(2,6-dimethyl- moφholin-4-ylmethyl)-quinazolin-4-yl]-amine; (R,R)-[2-(2,6-Dimethyl-moφholin-4- ylmethyl)-7-(3-1rifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(4-trifluoromethyl-phenyl)- amine; (R,R)-[2-(2,6-Dimethyl-moφholin-4-ylmethyl)-7-(3-trifluoromethyl-pyridin-2-yl)- quinazolin-4-yl]-(6-trifluoromethyl-pyridin-3-yl)-amine; (R,R)-[2-(2,6-Dimethyl-moφholin- 4-ylmethyl)-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(4-isopropyl-phenyl)-amine; (R,R)-[2-(2,6-Dimethyl-moφholin-4-ylmethyl)-7-(3-trifluoromethyl-pyridin-2-yl)- quinazolin-4-yl]-(4-ethyl-phenyl)-amine; (R,R)-[2-(2,6-Dimethyl-moφholin-4-ylmethyl)-7- (3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-[4-(propane-2-sulfonyl)-phenyl]-amine; (R,R)-[2-(2,6-Dimethyl-moφholin-4-ylmethyl)-7-(3-trifluoromethyl-pyridin-2-yl)- pyrido[3,2-d]pyrimidin-4-yl]-(4-trifluoromethyl-phenyl)-amine; (R,R)-[7-(3-Chloro-pyridin- 2-yl)-2-(2,6-dimethyl-moφholin-4-ylmethyl)-quinazolin-4-yl]-(4-trifluoromethyl-phenyl)- amine; (R,R)-[7-(3-Chloro-pyridin-2-yl)-2-(2,6-dimethyl-moφholin-4-ylmethyl)-quinazolin- 4-yl]-(6-trifluoromethyl-pyridin-3-yl)-amine; (R,R)-[7-(3-Chloro-pyridin-2-yl)-2-(2,6- dimethyl-moφholin-4-ylmethyl)-quinazolin-4-yl] -(6-isopropoxy-pyridin-3 -yl)-amine; (R,R)- [7-(3-Chloro-pyridin-2-yl)-2-(2,6-dimethyl-moφholin-4-ylmethyl)-quinazolin-4-yl]-[4- (propane-2-sulfonyl)-phenyl]-amine; (R,R)-l-{4-[7-(3-Chloro-pyridin-2-yl)-2-(2,6-dimethyl- moφholin-4-ylmethyl)-quinazolin-4-ylamino]-phenyl}-ethanone; (R,R)-4-[7-(3-Chloro- pyridin-2-yl)-2-(2,6-dimethyl-moφholin-4-ylmethyl)-quinazolin-4-ylamino]-benzonitrile; (S)-(4-Isopropyl-phenyl)-[2-(2-methyl-moφholin-4-ylmethyl)-7-(3-trifluoromethyl-pyridin- 2-yl)-quinazolin-4-yl] -amine; (S)-(4-tert-Butyl-phenyl)-[2-(2-methyl-moφholin-4-ylmethyl)- 7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-amine; (S)-(4-tert-Butyl-phenyl)-[7-(3- chloro-pyridin-2-yl)-2-(2-methyl-moφholin-4-ylmethyl)-quinazolin-4-yl]-amine; (S)-[2-(l- Propyl-pynolidin-2-yl)-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(4- trifluoromethyl-phenyl)-amine; (S)-[2-(2-Methyl-moφholin-4-ylmethyl)-7-(3- trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl] -(4-trifluoromethyl-phenyl)-amine; (S)- [2- Pynolidin-2-yl-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(4-trifluoromethyl- phenyl)-amine; (S)-[7-(3-Chloro-pyridin-2-yl)-2-(2-methyl-moφholin-4-ylmethyl)- quinazolin-4-yl]-[4-(propane-2-sulfonyl)-phenyl]-amine; (S)-[7-(3-Chloro-pyridin-2-yl)-2-(2- methyl-moφholin-4-ylmethyl)-quinazolin-4-yl]-(4-isopropyl-phenyl)-amine; (S,S)-(4-

Chloro-phenyl)-[2-(2,6-dimethyl-moφholin-4-ylmethyl)-7-(3-trifluoromethyl-pyridin-2-yl)- quinazolin-4-yl] -amine; (S,S)-(4-Chloro-phenyl)-[7-(3-chloro-pyridin-2-yl)-2-(2,6-dimethyl- moφholin-4-ylmethyl)-quinazolin-4-yl]-amine; (S,S)-(4-tert-Butyl-phenyl)-[7-(3-chloro- pyridin-2-yl)-2-(2,6-dimethyl-moφholin-4-ylmethyl)-quinazolin-4-yl]-amine; (S,S)-(6-tert- Butyl-pyridin-3-yl)-[2-(2,6-dimethyl-moφholin-4-ylmethyl)-7-(3-tfifluoromethyl-pyridin-2- yl)-quinazolin-4-yl]-amine; (S,S)-(6-tert-Butyl-pyridin-3-yl)-[7-(3-chloro-pyridin-2-yl)-2- (2,6-dimethyl-moφholin-4-ylmethyl)-quinazolin-4-yl]-amine; (S,S)-[2-(2,6-Dimethyl- moφholin-4-ylmethyl)-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(4- trifluoromethyl-phenyl)-amine; (S,S)-[2-(2,6-Dimethyl-moφholin-4-ylmethyl)-7-(3- tnfluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(6-trifluoromethyl-pyridin-3-yl)-amine; (S,S)- [2-(2,6-Dimethyl-moφholin-4-ylmethyl)-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]- (4-isopropyl-phenyl)-amine; (S,S)-[2-(2,6-Dimethyl-moφholin-4-ylmethyl)-7-(3- trifluoromethyl-pyridin-2 -yl)-quinazolin-4-yl]-(4-ethyl-phenyl)-amine; (S,S)-[2-(2,6-

Dimethyl-moφholin-4-ylmethyl)-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-[4- (propane-2-sulfonyl)-phenyl]-amine; (S,S)-[2-(2,6-Dimethyl-moφholin-4-ylmethyl)-7-(3- trifluoiOmethyl-pyridin-2-yl)-pyrido [3 ,2-d]pyrimidin-4-yl] ~(4-trifluoromethyl-phenyl)-amine; (S,S)-[7-(3-Chloro-pyridin-2-yl)-2-(2,6-dimethyl-moφholin-4-ylmethyl)-quinazolin-4-yl]-(4- trifluoromethyl-phenyl)-amine; (S,S)-[7-(3-Chloro-pyridin-2-yl)-2-(2,6-dimethyl-moφholin- 4-ylmethyl)-quinazolin-4-yl]-(6-trifluoromethyl-pyridin-3-yl)-amine; (S,S)-[7-(3-Chloro- pyridin-2-yl)-2-(2,6-dimethyl-moφholin-4-ylmethyl)-quinazolin-4-yl]-[4-(propane-2- sulfonyl)-phenyl] -amine; (S,S)-[7-(3-Chloro-pyridin-2-yl)-2-(2,6-dimethyl-moφholin-4- ylmethyl)-quinazolin-4-yl]-(4-isopropyl-phenyl)-amine; (S,S)-[7-(3-Chloro-pyridin-2-yl)-2- (2,6-dimethyl-moφholin-4-ylmethyl)-quinazolin-4-yl]-(4-ethyl-phenyl)-amine; [2-(l,l-

Dioxo- 1 λ⁶-[ 1 ,2]thiazinan-2-ylmethyl)-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(4- trifluoromethyl-phenyl)-amine; [2-(l , 1 -Dioxo- 1 λ -thiomoφholin-4-ylmethyl)-7-(3- trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(6-trifluoromethyl-pyridin-3-yl)-amine; [2- (l,4-Dioxa-8-aza-spiro[4.5]dec-8-ylmethyl)-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4- yl]-(4-trifluoromethyl-phenyl)-amine; [2-(l ,4-Dioxa-8-aza-spiro[4.5]dec-8-ylmethyl)-7-(3- trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(6-trifluoromethyl-pyridin-3-yl)-amine; [2-(l- Ethyl-piperidin-4-yl)-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(4-trifluoromethyl- phenyl)-amine; [2-( 1 -Methanesulfonyl-piperidin-4-yl)-7-(3 -trifluoromethyl-pyridin-2 -yl)- quinazolin-4-yl]-(4-trifluoromethyl-phenyl)-amine; [2-(l-Methyl-3,4-dihydro-lH- isoquinolin-2-ylmethyl)-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(4- trifluoromethyl-phenyl)-amine; [2-(l-Methyl-3,4-dihydro-lH-isoquinolin-2-ylmethyl)-7-(3- trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(6-trifluoromethyl-pyridin-3-yl)-amine; [2-(l- Propyl-piperidin-4-yl)-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(4-trifluoromethyl- phenyl)-amine; [2-(l-Pyridin-4-ylmethyl-piperidin-4-yl)-7-(3-trifluoromethyl-pyridin-2-yl)- quinazolin-4-yl]-(4-trifluoromethyl-phenyl)-amine; [2-(2,2-Dimethyl-moφholin-4-yhnethyl)- 7-(3-1rifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(4-1rifluoromethyl-phenyl)-amine; [2- (2,2-Dimethyl-moφholin-4-ylmethyl)-7-(3 -trifluoromethyl-pyridin-2-yl)-pyrido [3 ,2- d]pyrimidin-4-yl]-(4-trifluoromethyl-phenyl)-amine; [2-(2,6-Dimethyl-moφholin-4- ylmethyl)-7-(2-methoxy-phenyl)-quinazolin-4-yl]-(4-trifluoromethyl-phenyl)-amine (cis); [2- (2,6-Dimethyl-moφholin-4-ylmethyl)-7-(3-methyl-pyridin-2-yl)-pyrido[3,2-d]pyrimidin-4- yl]-(4-trifluoromethyl-phenyl)-amine (cis); [2-(2,6-Dimethyl-moφholin-4-ylmethyl)-7-(3- methyl-pyridin-2-yl)-quinazolin-4-yl] -(4-trifluoromethyl-phenyl)-amine (cis); [2-(2,6- Dimethyl-moφholin-4-ylmethyl)-7-(3-methyl-pyridin-2-yl)-pyrido[2,3-d]pyrimidin-4-yl]-(4- trifluoromethyl-phenyl)-amine; [2-(2,6-Dimethyl-moφholin-4-ylmethyl)-7-(3- trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(6-trifluoromethyl-pyridin-3-yl)-amine; [2- (2,6-Dimethyl-moφholin-4-ylmethyl)-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(4- trifluoromethyl-phenyl)-amine; [2-(2,6-Dimethyl-moφholin-4-ylmethyl)-7-(3- trifluoromethyl-pyridin-2 -yl)-quinazolin-4-yl]-(4-trifluoromethyl-phenyl)-amine (cis); [2-

(2,6-Dimethyl-moφholin-4-ylmethyl)-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(5- trifluoromethyl-pyridin-2-yl)-amine; [2-(2,6-Dimethyl-moφholin-4-ylmethyl)-7-(3- trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-[4-(2,2,2-trifluoro-l-methyl-ethyl)-phenyl]- amine; [2-(2,6-Dimethyl-moφhohn-4-ylmethyl)-7-(3-trifluoromethyl-pyridin-2-yl)- quinazolin-4-yl]-(6-trifluoromethyl-pyridin-3-yl)-amine (cis); [2-(2,6-Dimethyl-moφholin-4- ylmethyl)-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(5-trifluoromethyl-pyridin-2- yl)-amine (cis); [2-(2,6-Dimethyl-moφholin-4-ylmethyl)-7-(3-trifluoromethyl-pyridin-2-yl)- pyrido[3,2-d]pyrimidin-4-yl]-(4-trifluoromethyl-phenyl)-amine (cis); [2-(2,6-Dimethyl- moφholin-4-yhnethyl)-7-(3-trifluoromethyl-pyridin-2-yl)-pyrido[3,2-d]pyrimidin-4-yl]-(6- trifluoromethyl-pyridin-3-yl)-amine (cis); [2-(2,6-Dimethyl-moφholin-4-ylmethyl)-7-(3- trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(4-trifluoromethanesulfonyl-phenyl)-amine; [2-(2,6-Dimethyl-moφholin-4-ylmethyl)-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]- (4-trifluoromethanesulfonyl-phenyl)-amine (cis); [2-(2,6-Dimethyl-moφholin-4-ylmethyl)-7- (3-trifluoromethyl-pyridin-2-yl)-pyrido[3,2-d]pyrimidin-4-yl]-(4-methanesulfonyl-phenyl)- amine (cis); [2-(2,6-Dimethyl-moφholin-4-ylmethyl)-7-(3-trifluoromethyl-pyridin-2-yl)- pyrido[3,2-d]pyrimidin-4-yl]-(4-trifluoromethanesulfonyl-phenyl)-amine (cis); [2-(2,6- Dimethyl-moφholin-4-ylmethyl)-7-(3-tnfluoromethyl-pyridin-2-yl)-pyrido[3,2-d]pyrimidin- 4-yl]-[4-(propane-l-sulfonyl)-phenyl]-amine (cis); [2-(2,6-Dimethyl-moφholin-4-yhnethyl)- 7-(3 -trifluoromethyl-pyridin-2-yl)-pyrido [3 ,2-d]pyrimidin-4-yl] - [4-(propane-2-sulfonyl)- phenyl]-amine (cis)[2-(2,6-Dimethyl-moφholin-4-ylmethyl)-7-(3-trifluoromethyl-pyridin-2- yl)-pyrido[3,2-d]pyrimidin-4-yl]-(4-isopropyl-phenyl)-amine (cis); [2-(2,6-Dimethyl- moφholin-4-ylmethyl)-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(4-isopropyl- phenyl)-amine (cis); [2-(2,6-Dimethyl-moφholin-4-ylmethyl)-7-(3-trifluoromethyl-pyridin- 2-yl)-pyrido[2,3-d]pyrimidin-4-yl]-(4-trifluoromethyl-phenyl)-amine (cis); [2-(2,6-Dimethyl- moφholin-4-ylmethyl)-7-pyridin-2-yl-quinazolin-4-yl]-(4-trifluoromethyl-phenyl)-amine (cis); [2-(2-Ethyl-piperidin- 1 -ylethyl)-7-(3 -trifluoromethyl-pyridin-2 -yl)-quinazolin-4-yl] ~(4- trifluoromethyl-phenyl)-amine; [2-(2-Ethyl-piperidin-l-ylmethyl)-7-(3-trifluoromethyl- pyridin-2-yl)-quinazolin-4-yl] -(4-trifluoromethyl-phenyl)-amine; [2-(2-Ethyl-piperidin- 1 - ylmethyl)-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(6-trifluoromethyl-pyridin-3- yl)-amine; [2-(2-Methyl-piperidin-l-ylethyl)-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4- yl]-(4-trifluoromethyl-phenyl)-amine; [2-(2-Methyl-piperidin-l-ylmethyl)-7-(3- trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(4-trifluoromethyl-phenyl)-amine; [2-(2- Methyl-piperidin-l-ylmethyl)-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(6- trifluoromethyl-pyridin-3 -yl)-amine; [2-(3 ,3 -Dimethyl-piperidin- 1 -ylethyl)-7-(3- trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(4-trifluoromethyl-phenyl)-amine; [2-(3,3- Dimethyl-piperidin-l-ylmethyl)-7-(3-methyl-pyridin-2-yl)-quinazolin-4-yl]-(4-isopropyl- phenyl)-amine; [2-(3 ,3 -Dimethyl-piperidin- 1 -ylmethyl)-7-(3 -methyl-pyridin-2-yl)- quinazolin-4-yl]-(4-trifluoromethyl-phenyl)-amine; [2-(3, 3 -Dimethyl-pip eridin-1-ylmethyl)- 7-(3-methyl-pyridin-2-yl)-pyrido[2,3-d]pyrimidin-4-yl]-(4-trifluoromethyl-phenyl)-amine; [2-(3 ,3-Dimethyl-piperidin- 1 -ylmethyl)-7-(3 -trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl] - (4-trifluoromethyl-phenyl)-amine; [2-(3,3-Dimethyl-piperidin-l-ylmethyl)-7-(3- trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(6-trifluoromethyl-pyridin-3-yl)-amine; [2- (3 ,3 -Dimethyl-piperidin- 1 -ylmethyl)-7-(3 -trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl] -(4- trifluoromethyl-phenyl)-amine; [2-(3,3-Dimethyl-piperidin-l-ylmethyl)-7-(3-trifluoromethyl- pyridin-2-yl)-pyrido [3 ,2-d]pyrimidin-4-yl] -(4-trifluoromethyl-phenyl)-amine; [2-(3 ,3- Dimethyl-piperidin-l-ylmethyl)-7-(3-trifluoromethyl-pyridin-2-yl)-pyrido[2,3-d]pyrimidin-4- yl] -(4-trifluoromethyl-phenyl)-amine; [2-(3 ,4-Dihydro- 1 H-isoquinolin-2-ylmethyl)-7-(3 - trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl] -(4-trifluoromethyl-phenyl)-amine; [2-(3 ,4- Dihydro-lH-isoquinolin-2-ylmethyl)-7-(3-tnfluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(6- trifluoromethyl-pyridin-3-yl)-amine; [2-(3, 5 -Dimethyl-piperidin- l-ylethyl)-7-(3 - trifluoromethyl-pyridin-2 -yl)-quinazolin-4-yl]-(4-trifluoromethyl-phenyl)-amine; [2-(3,5- Dimethyl-piperidin-l-ylmethyl)-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(4- trifluoromethyl-ρhenyl)-amine; [2-(3,5-Dimethyl-piperidin-l-ylmethyl)-7-(3-trifluoromethyl- pyridin-2-yl)-quinazolin-4-yl]-(6-trifluoromethyl-pyridin-3-yl)-amine; [2-(3,5-Dimethyl- piperidin-l-ylmethyl)-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(4-trifluoromethyl- phenyl)-amine; [2-(3,5-Dimethyl-piperidin-l-ylmethyl)-7-(3-trifluoromethyl-pyridin-2-yl)- pyrido[3,2-d]pyrimidin-4-yl]-(4-trifluoromethyl-phenyl)-amine (cis); [2-(3, 5 -Dimethyl- piperidin- 1 -ylmethyl)-7-(3 -trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl] -(6-trifluoromethyl- pyridin-3-yl)-amine; [2-(3-Hydroxy-piperidin-l-ylethyl)-7-(3-trifluoromethyl-pyridin-2-yl)- quinazolin-4-yl]-(4-trifluoromethyl-phenyl)-amine; [2-(3-Methoxy-piperidin-l-ylethyl)-7-(3- trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(4-trifluoromethyl-phenyl)-amine; [2-(3- Methyl-piperidin-l-ylethyl)-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(4- trifluoromethyl-phenyl)-amine; [2-(3-Methyl-piperidin-l-ylmethyl)-7-(3-trifluoromethyl- pyridin-2-yl)-quinazolin-4-yl]-(6-trifluoromethyl-pyridin-3-yl)-amine; [2-(3-Methyl- piperidin- 1 -ylmethyl)-7-(3 -trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl] -(4-trifluoromethyl- phenyl)-amine; [2-(3-Methyl-piperidin-l-ylmethyl)-7-(3-trifluoromethyl-pyridin-2-yl)- pyrido [3 ,2-d]pyrimidin-4-yl]-(4-trifluoromethyl-phenyl)-amine; [2-(3 -Pynolidin- 1 -yl- propyl)-7-(3-tnfluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(4-trifluoromethyl-phenyl)- amine; [2-(4-Cyclopentyl-piperazin- 1 -ylmethyl)-7-(3-trifluoromethyl-pyridin-2-yl)- quinazolin-4-yl]-(4-trifluoromethyl-phenyl)-amine; [2-(4-Ethoxy-piperidin-l-ylethyl)-7-(3- trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(4-trifluoromethyl-phenyl)-amine; [2-(4-Ethyl- piperazin-l-ylmethyl)-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(4-trifluoromethyl- phenyl)-amine; [2-(4-Hydroxy-piperidin- 1 -ylethyl)-7-(3 -trifluoromethyl-pyridin-2-yl)- quinazolin-4-yl] -(4-trifluoromethyl-phenyl)-amine; [2-(4-Isopropyl-piperazin- 1 -ylmethyl)-7- (3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(4-trifluoromethyl-phenyl)-amine; [2-(4- Methoxy-piperidin-l-ylethyl)-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(4- trifluoromethyl-phenyl)-amine; [2-(4-Methyl-[l,4]diazepan-l-ylmethyl)-7-(3- trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(4-trifluoromethyl-phenyl)-amine; [2-(4- Methyl-piperazin-l-ylmethyl)-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(5- trifluoromethyl-pyridin-2-yl)-amine; [2-(4-Methyl-piperazin-l-ylmethyl)-7-(3- trifluoromethyl-pyridin-2 -yl)-quinazolin-4-yl] -[4-(2,2,2-trifluoro- 1 -methyl-ethyl)-phenyl] - amine; [2-(4-Methyl-piperazin-l-ylmethyl)-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4- yl]-(4-trifluoromethyl-phenyl)-amine; [2-(4-Methyl-piperidin-l-ylethyl)-7-(3- trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(4-trifluoromethyl-phenyl)-amine; [2-(4- Methyl-piperidin-l-ylmethyl)-7-(3-frifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(4- trifluoromethyl-phenyl)-amine; [2-(4-Methyl-piperidin-l-ylmethyl)-7-(3-trifluoromethyl- pyridin-2-yl)-quinazolin-4-yl]-(6-trifluoromethyl-pyridin-3-yl)-amine; [2-(5,6-Dihydro-4H- pyrimidin-l-ylmethyl)-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(4- trifluoromethyl-phenyl)-amine; [2-(5H-Tetrazol-5-yl)-7-(3-trifluoromethyl-pyridin-2-yl)- quinazolin-4-yl]-(4-trifluoromethyl-phenyl)-amine; [2-(6-Methoxy-pyridin-3-yl)-7-(3- trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(4-trifluoromethyl-phenyl)-amine; [2-(6- Pynolidin-l-yl-pyridin-3-yl)-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(4- trifluoromethyl-phenyl)-amine; [2-(Benzylamino-methyl)-7-(3-trifluoromethyl-pyridin-2-yl)- quinazolin-4-yl]-(5-trifluoromethyl-pyridin-2-yl)-amine; [2-(Benzylamino-methyl)-7-(3- trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl] -(6-trifluoromethyl-pyridin-3 -yl)-amine; [2- (Isobutylamino-methyl)-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(4- trifluoromethyl-phenyl)-amine; [2-(Isopropylamino-methyl)-7-(3-trifluoromethyl-pyridin-2- yl)-quinazolin-4-yl] -(4-trifluoromethyl-phenyl)-amine; [2-(Octahydro-quinolin- 1 -ylethyl)-7- (3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(4-trifluoromethyl-phenyl)-amine; [2- (Octahydro-quinolin-l-ylmethyl)-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(4- trifluoromethyl-phenyl)-amine; [2-(Octahydro-quinolin-l-ylmethyl)-7-(3-trifluoromethyl- pyridin-2-yl)-quinazolin-4-yl]-(6-trifluoromethyl-pyridin-3-yl)-amine; [2-(tert-Butylamino- methyl)-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(4-trifluoromethyl-phenyl)- amine; [2-[(2-Methoxy-benzylamino)-methyl]-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin- 4-yl] -(6-trifluoromethyl-pyridin-3 -yl)-amine; [2-[(2-Methoxy-ethylamino)-methyl] -7-(3 - tnfluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(4-trifluoromethyl-phenyl)-amine; [2-[(3- Methyl-butylamino)-methyl]-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(4- trifluoromethyl-phenyl)-amine; [2-[(4-Methoxy-benzylamino)-methyl]-7-(3-trifluoromethyl- pyridin-2-yl)-quinazolin-4-yl]-(6-trifluoromethyl-pyridin-3-yl)-amine; [2-[(Allyl-methyl- amino)-ethyl]-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(4-trifluoromethyl-phenyl)- amine; [2-[(Allyl-methyl-amino)-methyl]-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4- yl]-(4-trifluoromethyl-phenyl)-amine; [2-[(Allyl-methyl-amino)-methyl]-7-(3- trifluoromethyl-pyridin-2-yl)-pyrido[3,2-d]pyrimidin-4-yl]-(4-trifluoromethyl-phenyl)-amine; [2-[(Allyl-methyl-amino)-methyl]-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(6- trifluoromethyl-pyridin-3-yl)-amine; [2-[(Benzyl-cyclopropyl-amino)-methyl]-7-(3- trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(4-trifluoromethyl-phenyl)-amine; [2- [(Berrzyl-cyclopropyl-amino)-methyl]-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(6- trifluoromethyl-pyridin-3-yl)-amine; [2-[(Benzyl-methyl-amino)-methyl]-7-(3- trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(6-trifluoromethyl-pyridin-3-yl)-amine; [2- [(Benzyl-methyl-amino)-methyl]-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(4- trifluoromethyl-phenyl)-amine; [2-[(Benzyl-methyl-amino)-methyl]-7-(3-trifluoromethyl- pyridin-2-yl)-quinazolin-4-yl]-(6-trifluoromethyl-pyridin-3-yl)-amine; [2-[(Bis- ethoxymethyl-amino)-methyl] -7-(3 -methyl-pyridin-2-yl)-quinazolin-4-yl] -(4-trifluoromethyl- phenyl)-amine; [2-[(Butyl-ethyl-amino)-ethyl]-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin- 4-yl]-(4-trifluoromethyl-phenyl)-amine; [2-[(Butyl-ethyl-amino)-methyl]-7-(3- trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(4-trifluoromethyl-phenyl)-amine; [2-[(Butyl- ethyl-amino)-methyl]-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(6-trifluoromethyl- pyridin-3 -yl)-amine; [2-[(Butyl-methyl-amino)-ethyl]-7-(3-trifluoromethyl-pyridin-2-yl)- quinazolin-4-yl]-(4-trifluoromethyl-phenyl)-amine; [2-[(Butyl-methyl-amino)-methyl]-7-(3- trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(4-trifluoromethyl-phenyl)-amine; [2-[(Butyl- methyl-amino)-methyl]-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(6- trifluoromethyl-pyridin-3-yl)-amine; [2-[(Cyclohexyl-ethyl-amino)-ethyl]-7-(3- trifluoromethyl-pyridin-2-yl)-quinazohn-4-yl]-(4-trifluoromethyl-phenyl)-amine; [2- [(Cyclohexyl-ethyl-amino)-methyl]-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(4- trifluoromethyl-phenyl)-amine; [2-[(Cyclohexyl-ethyl-amino)-methyl]-7-(3-trifluoromethyl- pyridin-2-yl)-quinazolin-4-yl]-(6-trifluoromethyl-pyridin-3-yl)-amine; [2-[(Cyclohexyl- methyl-amino)-ethyl]-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(4-trifluoromethyl- phenyl)-amine; [2-[(Cyclohexyl-methyl-amino)-methyl]-7-(3-trifluoromethyl-pyridin-2-yl)- quinazolin-4-yl]-(4-trifluoromethyl-phenyl)-amine; [2-[(Cyclohexyl-methyl-amino)-methyl]- 7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(6-trifluoromethyl-pyridin-3-yl)-amine; [2-[(Cyclopropylmethyl-propyl-amino)-ethyl]-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin- 4-yl]-(4-trifluoromethyl-phenyl)-amine; [2-[(Cyclopropylmethyl-propyl-amino)-methyl]-7- (3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(4-trifluoromethyl-phenyl)-amine; [2- [(Cyclopropylmethyl-propyl-amino)-methyl]-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin- 4-yl]-(6-trifluoromethyl-pyridin-3-yl)-amine; [2-[(Cyclopropylmethyl-propyl-amino)- methyl]-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(4-trifluoromethyl-phenyl)- amine; [2-[(Cyclopropylmethyl-propyl-amino)-methyl]-7-(3-trifluoromethyl-pyridin-2-yl)- pyrido[3,2-d]pyrimidin-4-yl]-(4-trifluoromethyl-phenyl)-amine; [2-[(Cyclopropylmethyl- propyl-amino)-methyl] -7-(3 -trifluoromethyl-pyridin-2 -yl)-quinazolin-4-yl] -(6- trifluoromethyl-pyridin-3-yl)-amine; [2-[(Ethyl-isopropyl-amino)-methyl]-7-(3-methyl- pyridin-2-yl)-quinazolin-4-yl]-(4-isopropyl-phenyl)-amine; [2-[(Ethyl-isopropyl-amino)- methyl]-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(4-trifluoromethyl-phenyl)- amine; [2-[(Ethyl-isopropyl-amino)-methyl]-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4- yl]-(6-trifluoromethyl-pyridin-3-yl)-amine; [2-[(Ethyl-propyl-amino)-methyl]-7-(3-methyl- pyridin-2-yl)-quinazolin-4-yl] -(4-isopropyl-phenyl)-amine; [2-[(Ethyl-propyl-amino)- methyl]-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-[4-(moφholine-4-sulfonyl)- phenyl] -amine; [2-[(Hexyl-methyl-amino)-ethyl]-7-(3-trifluoromethyl-pyridin-2-yl)- quinazolin-4-yl]-(4-trifluoiOinethyl-phenyl)-amine; [2-[(Hexyl-methyl-amino)-methyl]-7-(3- trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(4-trifluoromethyl-phenyl)-amine; [2-[(Hexyl- methyl-amino)-methyl] -7-(3 -trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl] -(6- trifluoromethyl-pyridin-3-yl)-amine; [2-[(Indan-l-yl-methyl-amino)-methyl]-7-(3- trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(4-trifluoromethyl-phenyl)-amine; [2-[(hιdan- 1 -yl-methyl-amino)-methyl] -7-(3 -trifluoromethyl-pyridin-2 -yl)-quinazolin-4-yl] -(6- trifluoromethyl-pyridin-3-yl)-amine; [2-[(Isopropyl-ethyl-amino)-ethyl]-7-(3-trifluoromethyl- pyridin-2-yl)-quinazolin-4-yl]-(4-trifluoromethyl-phenyl)-amine; [2-[(Isopropyl-methyl- amino)-ethyl]-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(4-trifluoromethyl-phenyl)- amine; [2-[(Isopropyl-methyl-amino)-methyl]-7-(3-methyl-pyridin-2-yl)-quinazolin-4-yl]-(4- isopropyl-phenyl)-amine; [2-[(Isopropyl-methyl-amino)-methyl]-7-(3-trifluoromethyl- pyridin-2-yl)-quinazolin-4-yl]-(4-trifluoromethyl-phenyl)-amine; [2-[(Isopropyl-methyl- amino)-methyl]-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-[4-(moφholine-4- sulfonyl)-phenyl] -amine; [2-[(Isopropyl-methyl-amino)-methyl]-7-(3-trifluoromethyl- pyridin-2-yl)-quinazolin-4-yl]-(6-trifluoromethyl-pyridin-3-yl)-amine; [2-[(Methyl- phenethyl-amino)-methyl]-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(6- trifluoromethyl-pyridin-3-yl)-amine; [2-[(Methyl-propyl-amino)-methyl]-7-(3- trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(4-trifluoromethyl-phenyl)-amine; [2-

[(Methyl-propyl-amino)-methyl]-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-[4- (moφholine-4-sulfonyl)-phenyl]-amine; [2-[(Methyl-propyl-amino)-methyl]-7-(3- trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(6-trifluoromethyl-pyridin-3-yl)-amine; [2- [(Propyl-methyl-amino)-ethyl]-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(4- trifluoromethyl-phenyl)-amine; [2-[(Tetrahydro-thiopyran-4-ylamino)-methyl]-7-(3- trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(6-trifluoromethyl-pyridin-3-yl)-amine; [2-[l- (l-Methyl-lH-imidazol-2-ylmethyl)-piperidin-4-yl]-7-(3-trifluoromethyl-pyridin-2-yl)- quinazolin-4-yl]-(4-trifluoromethyl-phenyl)-amine; [2-[2-(l,4-Dioxa-8-aza-spiro[4.5]dec-8- yl)-ethyl]-7-(3-tfifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(4-trifluoromethyl-phenyl)- amine; [2-[2-(l-Methyl-3,4-dihydro-lH-isoquinolin-2-yl)-ethyl]-7-(3-trifluoromethyl- pyridin-2-yl)-quinazolin-4-yl]-(4-trifluoromethyl-phenyl)-amine; [2-[2-(2,6-Dimethyl- moφholin-4-yl)-ethyl]-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(4- trifluoromethyl-phenyl)-amine; [2- [2-(3 ,4-Dihydro- 1 H-isoquinolin-2-yl)-ethyl] -7-(3 - trifluoromethyl-pyridin-2 -yl)-quinazolin-4-yl]-(4-trifluoromethyl-phenyl)-amine; [2-[2-(4- Methyl-piperazin-l-yl)-ethyl]-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(4- trifluoromethyl-phenyl)-amine; [2-[2-(Benzyl-cyclopropyl-amino)-ethyl]-7-(3- trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(4-trifluoromethyl-phenyl)-amine; [2-[2- (Benzyl-methyl-amino)-ethyl]-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(4- trifluoromethyl-phenyl)-amine; [2-[2-(hιdan-l-yl-methyl-amino)-ethyl]-7-(3-trifluoromethyl- pyridin-2-yl)-qumazolin-4-yl]-(4-trifluoromethyl-phenyl)-amine; [2-[2-(Methyl-phenethyl- amino)-ethyl]-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(4-trifluoromethyl-phenyl)- amine; [2-[3-(2,6-Dimethyl-moφholin-4-yl)-propyl]-7-(3-trifluoromethyl-pyridin-2-yl)- quinazolin-4-yl]-(6-trifluoromethyl-pyridin-3-yl)-amine; [2-[3-(2,6-Dimethyl-moφholin-4- yl)-propyl]-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(4-trifluoromethyl-phenyl)- amine; [2-[3-(2,6-Dimethyl-moφholin-4-yl)-propyl]-7-(3-trifluoromethyl-pyridin-2-yl)- quinazolin-4-yl]-(4-trifluoromethyl-phenyl)-amine (cis); [2-[3-(3-Methyl-piperidin-l-yl)- propyl]-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(4-trifluoromethyl-phenyl)- amine; [2- [3-(4-Methyl-piperazin- 1 -yl)-propyl] -7-(3 -trifluoromethyl-pyridin-2-yl)- quinazolin-4-yl]-(4-trifluoromethyl-phenyl)-amine; [2-[4-(2-Diethylamino-ethyl)-piperazin- l-ylmethyl]-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(4-trifluoromethyl-phenyl)- amine; [2-[4-(2-Dimethylamino-ethyl)-piperazin-l-ylmethyl]-7-(3-trifluoromethyl-pyridin-2- yl)-quinazolin-4-yl]-(4-trifluoromethyl-phenyl)-amine; [2-[4-(2-Methoxy-ethyl)-piperazin-l- ylmethyl] -7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(4-trifluoromethyl-phenyl)- amine; [2-[4-(2-Mθφholin-4-yl-ethyl)-piperazin-l-ylmethyl]-7-(3-trifluoromethyl-pyridin-2- yl)-quinazolin-4-yl]-(4-trifluoromethyl-phenyl)-amine; [2-[4-(2-Pynolidin-l-yl-ethyl)- piperazin- 1 -ylmethyl] -7-(3 -trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl] -(4-trifluoromethyl- phenyl)-amine; [2-[4-(3-Dimethylamino-propyl)-piperazin-l-ylmethyl]-7-(3-trifluoromethyl- pyridin-2-yl)-quinazolin-4-yl] -(4-trifluoromethyl-phenyl)-amine; [2- { [(2-Fluoro-benzyl)- methyl-amino]-methyl}-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(4- trifluoromethyl-phenyl)-amine; [2- { [(2-Fluoro-benzyl)-methyl-amino] -methyl} -7-(3 - tfifluoromethyl-pyridin-2-yl)-quinazohn-4-yl]-(6-trifluoromethyl-pyridin-3-yl)-amine; [2- {[(3-Fluoro-berιzyl)-methyl-amino]-methyl}-7-(3-tnfluoromethyl-pyridin-2-yl)-quinazolin-4- yl]-(4-trifluoromethyl-phenyl)-amine; [2-{[(3-Fluoro-benzyl)-methyl-amino]-methyl}-7-(3- 1rifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(6-trifluoromethyl-pyridin-3-yl)-amine; [2- {[(Pyridin-2-ylmethyl)-amino]-methyl}-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]- (6-trifluoroinethyl-pyridin-3-yl)-amine; [2- {[Bis-(2-methoxy-ethyl)-amino]-methyl} -7-(3- methyl-pyridin-2-yl)-quinazolin-4-yl] -(4-isopropyl-phenyl)-amine; [2- { [Bis-(2-methoxy- ethyl)-amino]-methyl}-7-(3-methyl-pyridin-2-yl)-pyrido[2,3-d]pyrimidin-4-yl]-(4- trifluoromethyl-phenyl)-amine; [2-{[Bis-(2-methoxy-ethyl)-amino]-methyl}-7-(3- trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(5-trifluoromethyl-pyridin-2-yl)-amine; [2- {[Bis-(2-methoxy-ethyl)-amino]-methyl}-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4- yl]-[4-(2,2,2-trifluoro-l-methyl-ethyl)-phenyl]-amine; [2-{[Bis-(2-methoxy-ethyl)-amino]- methyl}-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(4-trifluoromethyl-phenyl)- amine; [2-{[Bis-(2-methoxy-ethyl)-aιnino]-methyl}-7-(3-trifluoromethyl-pyridin-2-yl)- pyrido[3,2-d]pyrimidin-4-yl]-(4-trifluoromethyl-phenyl)-amine; [2-{[Bis-(2-methoxy-ethyl)- amino]-methyl}-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(6-trifluoromethyl- pyridin-3-yl)-amine; [2-{[Bis-(2-methoxy-ethyl)-amino]-methyl}-7-(3-trifluoromethyl- pyridin-2-yl)-pyrido[2,3-d]pyrimidin-4-yl]-(4-trifluoromethyl-phenyl)-amine; [2-{[Ethyl-(2- methyl-allyl)-amino] -methyl} -7-(3 -trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl] -(4- trifluoromethyl-phenyl)-amine; [2-{[Ethyl-(2-methyl-allyl)-amino]-methyl}-7-(3- trifluoromethyl-pyridin-2 -yl)-pyrido[3,2-d]pyrimidin-4-yl]-(4-trifluoromethyl-phenyl)-amine; [2-{[Ethyl-(2-methyl-allyl)-amino]-methyl}-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4- yl]-(6-trifluoromethyl-pyridin-3-yl)-amine; [2-{[Methyl-(l-phenyl-ethyl)-amino]-methyl}-7- (3 -trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl] -(4-trifluoromethyl-phenyl)-amine; [2- { [Methyl-( 1 -phenyl-ethyl)-amino]-methyl} -7-(3 -trifluoromethyl-pyridin-2-yl)-quinazolin-4- yl]-(6-trifluoromethyl-pyridin-3-yl)-amine; [2-{[Methyl-(l-phenyl-propyl)-amino]-methyl}- 7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(4-trifluoromethyl-phenyl)-amine; [2- {[Methyl-(l-phenyl-propyl)-ammo]-methyl}-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4- yl]-(6-trifluoromethyl-pyridin-3-yl)-amine; [2-{[Methyl-(2-methyl-benzyl)-amino]-methyl}- 7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(4-trifluoromethyl-phenyl)-amine; [2- {[Methyl-(2-methyl-benzyl)-amino]-methyl}-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin- 4-yl]-(6-trifluoromethyl-pyridin-3-yl)-amine; [2- {[Methyl-(2-phenyl-ethyl)-amino] -methyl} - 7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(4-trifluoromethyl-phenyl)-amine; [2-{2- [(2-Fluoro-berιzyl)-methyl-amino]-ethyl}-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4- yl]-(4-trifluoromethyl-phenyl)-amine; [2- {2-[(3-Fluoro-benzyl)-methyl-amino]-ethyl} -7-(3- trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(4-trifluoromethyl-phenyl)-amine; [2-{2-[Bis- (2-methoxy-ethyl)-amino]-ethyl}-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(4- trifluoromethyl-phenyl)-amine; [2-{2-[Ethyl-(2-methyl-allyl)-amino]-ethyl}-7-(3- trifluoromethyl-pyridin-2 -yl)-quinazolin-4-yl]-(4-trifluoromethyl-phenyl)-amine; [2- {2-

[Methyl-(l-phenyl-ethyl)-amino]-ethyl}-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]- (4-trifluoromethyl-phenyl)-amine; [2-{2-[Methyl-(l-phenyl-propyl)-amino]-ethyl}-7-(3- trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(4-trifluoromethyl-phenyl)-amine; [2-{2- [Methyl-(2-methyl-benzyl)-amino]-ethyl}-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4- yl]-(4-trifluoromethyl-phenyl)-amine; [2-Azepan- l-ylethyl-7-(3-trifluoromethyl-pyridin-2- yl)-quinazolin-4-yl] -(4-trifluoromethyl-phenyl)-amine; [2-Azepan- 1 -ylmethyl-7-(3 - trifluoromethyl-pyridin-2 -yl)-pyrido [3 ,2-d]pyrimidin-4-yl] -(4-trifluoromethyl-phenyl)-amine; [2-Azepan-l-ylmethyl-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(4- trifluoromethyl-phenyl)-amine; [2-Azepan- 1 -ylmethyl-7-(3 -trifluoromethyl-pyridin-2-yl)- quinazolin-4-yl]-(6-trifluoromethyl-pyridin-3-yl)-amine; [2-Azetidin-3-yl-7-(3-methyl- pyridin-2-yl)-pyrido[2,3-d]pyrimidin-4-yl]-(4-trifluoromethyl-phenyl)-amine; [2-Azocan-l- ylethyl-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(4-trifluoromethyl-phenyl)-amine; [2-Azocan-l-ylmethyl-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(4- trifluoromethyl-phenyl)-amine; [2-Azocan- 1 -ylmethyl-7-(3-trifluoromethyl-pyridin-2-yl)- quinazolin-4-yl]-(6-trifluoromethyl-pyridin-3-yl)-amine; [2-Cyclohexylaminomethyl-7-(3- trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(4-trifluoromethyl-phenyl)-amine; [2- Diallylaminoethyl-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(4-trifluoromethyl- phenyl)-amine; [2-Diallylaminomethyl-7-(3-trifluoromethyl-pyridin-2-yl)-pyrido[3,2- d]pyrimidin-4-yl]-(4-trifluoromethyl-phenyl)-amine; [2-Diallylaminomethyl-7-(3- trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl] -(4-trifluoromethyl-phenyl)-amine; [2-

Diallylaminomethyl-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(6-trifluoromethyl- pyridin-3 -yl)-amine; [2-Dibutylaminoethyl-7-(3 -trifluoromethyl-pyridin-2-yl)-quinazolin-4- yl]-(4-trifluoromethyl-phenyl)-amine; [2-Dibutylaminomethyl-7-(3-trifluoromethyl-pyridin- 2-yl)-pyrido[3,2-d]pyrimidin-4-yl]-(4-trifluoromethyl-phenyl)-amine; [2- Dibutylaminomethyl-7-(3 -trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl] -(4-trifluoromethyl- phenyl)-amine; [2-Dibutylaminomethyl-7-(3 -trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl] - (6-trifluoromethyl-pyridin-3-yl)-amine; [2-Diethylaminoethyl-7-(3-trifluoromethyl-pyridin-2- yl)-quinazolin-4-yl]-(4-trifluoromethyl-phenyl)-amine; [2-Diethylaminomethyl-7-(3- tιifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(4-trifluoromethyl-phenyl)-amine; [2- Diethylaminomethyl-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(6-trifluoromethyl- pyridin-3-yl)-amine; [2-Dihexylaminoethyl-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4- yl] -(4-trifluoromethyl-phenyl)-amine; [2-Dihexylaminomethyl-7-(3 -trifluoromethyl-pyridin- 2-yl)-quinazolin-4-yl]-(4-trifluoromethyl-phenyl)-amine; [2-Dihexylaminomethyl-7-(3- 1xifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(6-trifluoromethyl-pyridin-3-yl)-amine; [2- Dimethylaminoethyl-7-(3-tfifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(4-trifluoromethyl- phenyl)-amine; [2-Dimethylaminomethyl-7-(3 -methyl-pyridin-2-yl)-quinazolin-4-yl] -(4- isopropyl-phenyl)-amine; [2-Dimethylaminomethyl-7-(3-trifluoromethyl-pyridin-2-yl)- quinazolin-4-yl]-(4-trifluoromethyl-phenyl)-amine; [2-Dimethylaminomethyl-7-(3- trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-[4-(moφholine-4-sulfonyl)-phenyl]-amine; [2- Dimethylaminomethyl-7-(3-tnfluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(6- trifluoromethyl-pyridin-3 -yl)-amine; [2-Dipentylaminoethyl-7-(3 -trifluoromethyl-pyridin-2- yl)-quinazolin-4-yl]-(4-trifluoromethyl-phenyl)-amine; [2-Dipentylaminomethyl-7-(3- trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(4-trifluoromethyl-phenyl)-amine; [2-

Dipentylaminomethyl-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(6-trifluoromethyl- pyridin-3 -yl)-amine; [2-Dipropylaminoethyl-7-(3 -trifluoromethyl-pyridin-2-yl)-quinazolin-4- yl]-(4-trifluoromethyl-phenyl)-amine; [2-Dipropylaminomethyl-7-(3-methyl-pyridin-2-yl)- quinazolin-4-yl]-(4-trifluoromethyl-phenyl)-amine; [2-Dipropylaminomethyl-7-(3- trifluoromethyl-pyridin-2 -yl)-pyrido[3,2-d]pyrimidin-4-yl]-(4-trifluoromethyl-phenyl)-amine; [2-Dipropylaminomethyl-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(6- trifluoromethyl-pyridin-3 -yl)-amine; [2-Dipropylaminomethyl-7-(3 -trifluoromethyl-pyridin- 2-yl)-quinazolin-4-yl]-(4-trifluoromethyl-phenyl)-amine; [2-Dipropylaminomethyl-7-(3- trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(6-trifluoromethyl-pyridin-3-yl)-amine; [2- Ethylaminomethyl-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(4-trifluoromethyl- phenyl)-amine; [2-Hexylaminomethyl-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(4- trifluoromethyl-phenyl)-amine; [2-hnidazol-l-ylmethyl-7-(3-trifluoromethyl-pyridin-2-yl)- pyrido[3,2-d]pyrimidin-4-yl]-(4-trifluoromethyl-phenyl)-amine; [2-Imidazol- l-ylmethyl-7- (3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(6-trifluoromethyl-pyridin-3-yl)-amine; [2- hnidazol- 1 -ylmethyl-7-(3 -trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl] -(4-trifluoromethyl- phenyl)-amine; [2-Methylaminomethyl-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]- (4-trifluoromethyl-ρhenyl)-amine; [2-Moφholin-4-ylethyl-7-(3-trifluoromethyl-pyridin-2- yl)-quinazolin-4-yl]-(4-trifluoromethyl-phenyl)-amine; [2-Mθφholin-4-ylmethyl-7-(3- trifluoromethyl-pyridin-2-yl)-pyrido[3,2-d]pyrimidin-4-yl]-(6-trifluoromethyl-pyridin-3-yl)- amine; [2-Mθφholin-4-ylmethyl-7-(3-trifluoromethyl-pyridin-2-yl)-pyrido[3,2-d]pyrimidin- 4-yl]-(6-trifluoromethyl-pyridin-2-yl)-amine; [2-Moφholin-4-ylmethyl-7-(3-trifluoromethyl- pyridin-2-yl)-quinazolin-4-yl]-(5-trifluoromethyl-pyridin-2-yl)-amine; [2-Moφholin-4- ylmethyl-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-[4-(2,2,2-trifluoro-l-methyl- ethyl)-phenyl] -amine; [2-Mθφholin-4-ylmethyl-7-(3-trifluoromethyl-pyridin-2-yl)- quinazolin-4-yl]-(6-trifluoromethyl-pyridin-3-yl)-amine; [2-Mθφholin-4-ylmethyl-7-(3- trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(4-trifluoromethanesulfonyl-plιenyl)-amine; [2-Mθφholin-4-ylmethyl-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(4- trifluoromethyl-phenyl)-amine; [2-Octylaminomethyl-7-(3-trifluoromethyl-pyridin-2-yl)- quinazolin-4-yl]-(4-trifluoromethyl-phenyl)-amine; [2-Piperidin-l-ylethyl-7-(3- trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(4-trifluoromethyl-phenyl)-amine; [2- Piperidin-l-ylmethyl-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-[4-(2,2,2-trifluoro- 1 -methyl-ethyl)-phenyl]-amine; [2-Piperidin- 1 -ylmethyl-7-(3-trifluoromethyl-pyridin-2-yl)- quinazolin-4-yl]-(4-trifluoromethyl-phenyl)-amine; [2-Piperidin-l-ylmethyl-7-(3-

1rifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(6-trifluoromethyl-pyridin-3-yl)-amine; [2- Piperidin-4-yl-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(4-trifluoromethyl- phenyl)-amine; [2-Pyridin-3-yl-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(4- trifluoromethyl-phenyl)-amine; [2-Pyridin-4-yl-7-(3-trifluoromethyl-pyridin-2-yl)- quinazolin-4-yl]-(4-trifluoromethyl-phenyl)-amine; [2-Pynolidin-l-ylmethyl-7-(3- trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(6-trifluoromethyl-pyridin-3-yl)-amine; [2- Thiomoφholin-4-ylethyl-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(4- trifluoromethyl-phenyl)-amine; [2-Thiomoφholin-4-ylmethyl-7-(3-trifluoromethyl-pyridin-2- yl)-pyrido[3,2-d]pyrimidin-4-yl]-(4-trifluoromethyl-phenyl)-amine; [2-Thiomoφholin-4- ylmethyl-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(6-trifluoromethyl-pyridin-3- yl)-amine; [2-Thiomoφholin-4-ylmethyl-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]- (4-trifluoromethyl-phenyl)-amine; [7-(3-Chloro-pyridin-2-yl)-2-(2,2-dimethyl-moφholin-4- ylmethyl)-quinazolin-4-yl]-(4-trifluoromethyl-phenyl)-amine; [7-(3-Chloro-pyridin-2-yl)-2- (2,6-dimethyl-moφholin-4-yhnethyl)-quinazolin-4-yl]-(4-trifluoromethyl-phenyl)-amine (cis); [7-(3-Chloro-pyridin-2-yl)-2-(2,6-dimethyl-moφholin-4-ylmethyl)-quinazolin-4-yl]-(6- trifluoromethyl-pyridin-3 -yl)-amine (cis); [7-(3 -Chloro-p yridin-2-yl)-2-(2,6-dimethyl- moφholin-4-ylmethyl)-quinazolin-4-yl]-(4-trifluoromethanesulfonyl-phenyl)-amine (cis); [7- (3-Chloro-pyridin-2-yl)-2-(2,6-dimethyl-moφholin-4-ylmethyl)-pyrido[3,2-d]pyrimidin-4- yl]-(4-trifluoromethyl-phenyl)-amine (cis); [7-(3-Chloro-pyridin-2-yl)-2-(2,6-dimethyl- moφholin-4-ylmethyl)-pyrido[3,2-d]pyrimidin-4-yl]-(6-trifluoromethyl-pyridin-3-yl)-amine (cis); [7-(3-Chloro-pyridin-2-yl)-2-(2,6-dimethyl-moφholin-4-ylmethyl)-pyrido[3,2- d]pyrimidin-4-yl]-(4-trifluoromethanesulfonyl-phenyl)-amine (cis); [7-(3-Chloro-pyridin-2- yl)-2-(2,6-dimethyl-moφholin-4-ylmethyl)-quinazolin-4-yl]-(4-isopropyl-phenyl)-amine (cis); [7-(3-Chloro-pyridin-2-yl)-2-(2,6-dimethyl-moφholin-4-ylmethyl)-pyrido[3,2- d]pyrimidin-4-yl]-[4-(moφholine-4-sulfonyl)-phenyl]-amine; [7-(3-Chloro-pyridin-2-yl)-2- (3,5-dimethyl-piperazin-l-ylmethyl)-quinazolin-4-yl]-(4-trifluoromethyl-phenyl)-amine; [7- (3 -Chloro-pyridin-2-yl)-2-imidazol- 1 -ylmethyl-pyrido [3 ,2-d]pyrimidin-4-yl]-(4- trifluoromethyl-phenyl)-amine; {l-[4-(4-tert-Butyl-phenylamino)-7-(3-trifluoromethyl- pyridin-2-yl)-quinazolin-2-ylmethyl]-pynolidin-2-yl} -methanol; { 1 -[4-(4-Trifluoromethyl- phenylamino)-7-(3 -trifluoromethyl-pyridin-2 -yl)-quinazolin-2-ylmethyl] -piperidin-4-yl} - methanol; { 1 -[4-(4-Trifluoroιnethyl-phenylamino)-7-(3-trifluoromethyl-pyridin-2-yl)- quinazolin-2-ylmethyl]-piperidin-2-yl} -methanol; {l-[4-(4-Trifluoromethyl-phenylamino)-7- (3-trifluoromethyl-pyridin-2-yl)-quinazolin-2-ylmethyl]-piperidin-3-yl}-methanol; {l-[7-(3- Methyl-pyridin-2-yl)-4-(4-trifluoromethyl-phenylamino)-quinazolin-2-ylmethyl]-pynolidin- 3-yl} -methanol (chiral); {l-[7-(3-Methyl-pyridin-2-yl)-4-(4-trifluoromethyl-phenylamino)- quinazolin-2-ylmethyl]-pynolidin-3-yl} -methanol (chiral); {l-[7-(3-Trifluoroιnethyl-pyridin- 2-yl)-4-(6-trifluoromethyl-pyridin-3-ylamino)-quinazolin-2-ylmethyl]-piperidin-4-yl}- methanol; {l-[7-(3-Trifluoromethyl-pyridin-2-yl)-4-(6-trifluoromethyl-pyridin-3-ylamino)- quinazolin-2-ylmethyl]-piperidin-3-yl} -methanol; 1 -[4-(4-Trifluoromethyl-phenylamino)-7- (3-trifluoromethyl-pyridin-2-yl)-quinazolin-2-ylmethyl]-piperidin-4-ol; l-[4-(4- Trifluoromethyl-phenylamino)-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-2-ylmethyl]- piperidin-3-ol; l-[4-(4-Trifluoromethyl-phenylamino)-7-(3-trifluoromethyl-pyridin-2-yl)- quinazolin-2-ylmethyl]-piperidine-4-carboxylic acid amide; l-[7-(3-Methyl-pyridin-2-yl)-4- (4-trifluoromethyl-phenylamino)-quinazolin-2-ylmethyl]-pynolidin-3-ol (chiral); l-[7-(3- Trifluoromethyl-pyridin-2-yl)-4-(6-trifluoromethyl-pyridin-3-ylamino)-quinazolin-2- ylmethyl]-piperidin-4-ol; l-[7-(3-Trifluoromethyl-pyridin-2-yl)-4-(6-trifluoromethyl-pyridin- 3-ylamino)-quinazolin-2-ylmethyl]-piperidin-3-ol; l-[7-(3-Trifluoromethyl-pyridin-2-yl)-4- (6-trifluoromethyl-pyridin-3-ylamino)-quinazolin-2-ylmethyl]-piperidine-4-carboxylic acid amide; l-{2-[2-(2,6-Dimethyl-moφholin-4-ylmethyl)-4-(4-trifluoromethyl-phenylamino)- quinazolin-7-yl] -phenyl} -ethanone (cis); 1- {2-[4-(4-Trifluoromethyl-phenylamino)-7-(3- trifluoromethyl-pyridin-2-yl)-quinazolin-2-yl]-ethyl}-piperidine-4-carboxylic acid amide; 1- {3-[4-(4-Trifluoromethyl-phenylamino)-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-2-yl]- propyl-piperidin-4-ol; 1 - {3-[4-(4-Trifluoromethyl-phenylamino)-7-(3-trifluoromethyl- pyridin-2-yl)-quinazolin-2-yl]-propyl}piperidin-3-ol; 1 - {4-[2-(2,6-Dimethyl-moφholin-4- ylmethyl)-7-(3-trifluoromethyl-pyridin-2-yl)-pyrido[3,2-d]pyrimidin-4-ylamino]-phenyl}- ethanone (cis); l-{4-[4-(4-Trifluoromethyl-phenylamino)-7-(3-trifluoromethyl-pyridin-2-yl)- quinazolin-2-yl] -piperidin- 1 -yl} -ethanone; 1 - {4-[4-(4-Trifluoromethyl-phenylamino)-7-(3 - trifluoromethyl-pyridin-2-yl)-quinazolin-2-yl]-piperidin-l -yl} -propan- 1 -one; 1 - {4-[7-(3- Trifluoromethyl-pyridin-2-yl)-4-(6-trifluoromethyl-pyridin-3-ylamino)-quinazolin-2- ylmethyl] -piperazin-1-yl} -ethanone; l-Pynolidin-l-yl-3-[4-(4-trifluoromethyl-phenylamino)- 7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-2-yl]-propan- 1 -one; 2-(l - {3-[4-(4-

Trifluoromethyl-phenylamino)-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-2-yl]-propyl}- piperidin-4-yl)-ethanol; 2-{[4-(4-tert-Butyl-phenylamino)-7-(3-trifluoromethyl-pyridin-2-yl)- quinazolin-2-ylmethyl]-propyl-amino}-ethanol; 2-{[4-(4-Trifluoromethyl-phenylamino)-7- (3-trifluoromethyl-pyridin-2-yl)-quinazolin-2-ylmethyl]-amino}-ethanol; 2-{l-[4-(4- Trifluoromethyl-phenylamino)-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-2-ylmethyl]- piperidin-4-yl} -ethanol; 2- { 1 -[7-(3-Trifluoromethyl-pyridin-2-yl)-4-(6-trifluoromethyl- pyridin-3-ylamino)-quinazolin-2-ylmethyl]-piperidin-4-yl} -ethanol; 2-{4-[4-(4- Trifluoromethyl-phenylamino)-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-2-ylmethyl]- piperazin-1-yl} -ethanol; 2-{4-[7-(3-Trifluoromethyl-pyridin-2-yl)-4-(6-trifluoromethyl- pyridin-3-ylamino)-quinazolin-2-ylmethyl]-piperazin-l-yl} -ethanol; 2-Methyl-2-{[4-(4- trifluoromethyl-phenylamino)-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-2-ylmethyl]- amino} -propan- 1 -ol; 4-(4-Trifluoromethyl-phenylamino)-7-(3-trifluoromethyl-pyridin-2-yl)- quinazoline-2-carboxylic acid dimethylamide; 4-(4-Trifluoromethyl-phenylamino)-7-(3- trifluoromethyl-pyridin-2-yl)-quinazoline-2-carboxylic acid methylamide; 4-(4- Trifluoromethyl-phenylamino)-7-(3-trifluoromethyl-pyridin-2-yl)-quinazoline-2-carboxylic acid (2-dimethylamino-ethyl)-amide; 4-(4-Trifluoromethyl-phenylamino)-7-(3- trifluoromethyl-pyridin-2-yl)-quinazoline-2-carboxylic acid (2-moφholin-4-yl-ethyl)-amide; 4-{2-[4-(4-Trifluoromethyl-phenylamino)-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-2- yl]-ethyl}-piperazine-l-carbaldehyde; N,N,N'-Trimethyl-N'-[4-(4-trifluoromethyl- phenylamino)-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-2-ylmethyl]-propane-l,3- diamine; and N-[4-(4-Trifluoromethyl-phenylamino)-7-(3-trifluoromethyl-pyridin-2-yl)- quinazolin-2-ylmethyl]-methanesulfonamide.

61. A compound of the formula

or a phanriaceutically acceptable salt thereof, wherein:

V, X, W, Y and Z are each independently N or CR_l5 with the proviso that at least one of V and X is N;

Ri is independently selected at each occurrence from hydrogen, halogen, hydroxy, cyano, amino, Ci-C₈alkyl, halod-C₈alkyl, d-C₈alkoxy, haloCι-C₈alkoxy and mono-and di-(Cι-

C₆alkyl) amino;

Ar and Ar₂ are independently selected from phenyl and 5- to 7-membered aromatic heterocycles, each of which is unsubstituted or substituted with from 1 to 3 substituents independently selected from groups ofthe formula LR_a;

L is independently selected at each occunence from a bond, -O-, -C(=O)-, -OC(=O)-, -

C(=0)O-, -O-C(=O)O-, -S(O)_m-, -NR_X-, -C(=0)NHR_x-, -NHR_xC(=O)-, -NR_xS(O)_m-, -

S(O)_mNR_x- and -N[S(O)_mR_x]S(O)_m-; wherein m is independently selected at each occunence from 0, 1 and 2; and R_x is independently selected at each occunence from hydrogen and d-C₈alkyl;

R_a is independently selected at each occunence from:

(i) hydrogen, halogen, cyano and nitro; and

(ii) Ci-C₈alkyl, C₂-C₈alkenyl, C₂-C₈alkynyl, C₂-C₈alkyl ether, 3- to 10-membered heterocycles, mono- and di-(Ci-C₈alkyl)amino and (3- to 10-membered heterocycle)Cι-C_alkyl, each of which is unsubstituted or substituted with from 1 to 9 substituents independently selected from R ;

R₃ is selected firom:

(i) hydrogen; (ii) Cι-C₈alkyl, d-C_.alkenyl, C₂-C₈alkynyl, C₂-C₈alkanone, C₂-C₈alkyl ether, C₆-

Ci₀arylCo-C₈alkyl, and 5- to 10-membered heterocycleCo-C_.alkyl, each of which is optionally substituted with from 1 to 9 substituents independently selected from R_b; and

(iii) groups that are joined to an R₅ or R to form a 4- to 10-membered heterocyclic group that is unsubstituted or substituted with firom 1 to 6 substituents independently selected R₅ and Rg are, independently at each occunence: (i) each independently selected from:

(a) hydrogen or hydroxy;

(b) d-C₈alkyl, unsubstituted or substituted with 1 or 2 substituents independently selected from R_b; and

(c) groups that are joined to R₃ to form a 4- to 10-membered heterocyclic group that is unsubstituted or substituted with from 1 to 6 substituents independently selected

(ii) joined to form a 3- to 7-membered carbocychc or heterocyclic ring, unsubstituted or substituted with from 1 to 4 substituents selected from R_b;

R_b is independently chosen at each occunence from hydroxy, halogen, amino, aminocarbonyl, amido, cyano, nitro, d-C₈alkyl, d-C₈alkoxy, d-C₈alkylthio, d-C₈alkyl ether, hydroxyd-C_.alkyl, halod-C_.alkyl, phenyl, phenyl(Ci-C_.alkyl), mono-and di-(d-

C₆alkyl)amino, (SO )Ci-C_alkyl, 5- to 7-membered heterocycle and (5- to 7-membered heterocycle)(Cι-C₈alkyl); and n is 1, 2 or 3.

62. A compound or salt according to claim 61, wherein V and X are both N.

63. A compound or salt according to claim 61, wherein W is N and Y and Z are

CH.

64. A compound or salt according to claim 61, wherein Y is N and W and Z are CH.

65. A compound or salt according to claim 61, wherein Z is N and W and Y are CH.

66. A compound or salt according to claim 61, wherein W, Y and Z are each CH.

67. A compound or salt according to claim 61, wherein Ari and Ar₂ are independently selected from phenyl and 6-membered aromatic heterocycles, each of which is substituted with 0, 1 or 2 substituents.

68. A compound or salt according to claim 67, wherein:

(i) Ari is phenyl or pyridyl, each of which is unsubstituted or substituted with 1 or 2 substituents selected from halogen, hydroxy, cyano, amino, nitro, mono- and di-(C_- C₆alkyl)amino, d-C₆alkyl, haloCi-C₆alkyl, d-C₆alkoxy and haloCi-C₆alkoxy; and

(ii) Ar₂ is phenyl or pyridyl, each of which is unsubstituted or substituted with 1 or 2 substituents selected from halogen, hydroxy, cyano, amino, nitro, mono- and di-(d- C₆alkyl)amino, Ci-C₆alkyl, halod-C₆alkyl, Ci-C₆alkoxy, halod-Cealkoxy, C₂-C₆alkyl ether, d-C₆alkanoyl, -(SO₂)R₂, -NR_xS(O)_m-, and -N(S(O_m)₂; wherein m is 1 or 2, R_x is hydrogen or Cι-C₆alkyl, and R₂ is d-C₆alkyl, halod-C₆all yl, amino, mono- or di-(d-C₆alkyl)amino or a 5- to 10-membered, N-linked heterocyclic group, each of which R₂ is optionally substituted with R_b.

69. A compound or salt according to claim 68, wherein: (i) Ari is pyridyl, unsubstituted or substituted with halogen, d-C alkyl or halod-

C₄alkyl; and

(ii) Ar₂ is phenyl or pyridyl, unsubstituted or substituted with Cι-C alkyl, halod- C₄alkyl or a group ofthe formula -(S0₂)R₂, wherein R₂ is C₁-C₄alkyl or halod-C alkyl.

70. A compound or salt according to claim 69, wherein:

(i) Ari is pyridin-2-yl, 3-methyl-pyridin-2-yl, 3-trifluoromethyl-pyridin-2-yl or 3- halo-pyridin-2-yl; and

(ii) Ar is phenyl, 2-pyridyl or 3 -pyridyl, each of which is substituted at the 4- position with trifluoromethanesulfonyl, propanesulfonyl, propane-2-sulfonyl, t-butyl, trifluoromethyl or 2,2,2-trifluoro-l -methyl-ethyl.

71. A compound or salt according to claim 61, wherein R₃ is: (i) hydrogen; or

(ii) Ci-C₈alkyl, C -C₈alkenyl, phenylC₀-C alkyl, 5- to 6-membered heteroarylC₀-C₄alkyl, or 4- to 7-membered heterocycloalkylC₀-C alkyl, each of which is unsubstituted or substituted with from 1 to 4 substituents independently selected from hydroxy, halogen, amino, Cι-C₆alkyl, halod-C₆alkyl, d-C₆alkoxy and halod-C₆alkoxy.

72. A compound or salt according to claim 71, wherein R₃ is: (i) hydrogen; or

(ii) Cι-C₆alkyl or benzyl, each of which is unsubstituted or substituted with from 1 to 3 substituents independently selected firom hydroxy, halogen and Ci-C₄alkyl.

73. A compound or salt according to claim 61, wherein each R₅ and R₆ is independently selected from hydrogen and d-C_όalkyl.

74. A compound or salt according to claim 73, wherein each R₅ and R₆ is hydrogen.

75. A compound or salt according to claim 61, wherein n is 1.

76. A compound or salt according to claim 61, wherein: (i) V and X are N;

(ii) Ari is pyridyl, unsubstituted or substituted with halogen, d-C₄alkyl or halod- C alkyl;

(iii) Ar₂ is phenyl or pyridyl, unsubstituted or substituted with d-C₄alkyl, halod- C₄alkyl or a group ofthe formula -(SO )R₂, wherein R₂ is d-C₄alkyl or haloCι-C₄alkyl; (iv) R is selected from (a) hydrogen; and (b) Cι-C₆alkyl, C₂-C₆alkenyl and phenylC₀-C alkyl, each of which is substituted with 0, 1 or 2 substituents independently selected from hydroxy, halogen, Cι-C₄alkyl and haloCι-C alkyl; and

(v) n is i.

77. A compound or salt according to claim 61, wherein the compound is selected from:

(l-Methanesulfonyl-2,3-dihydro-lH-indol-5-yl)-[2-methoxymethyl-7-(3-trifluoromethyl- pyridin-2-yl)-quinazolin-4-yl]-amine; (2,6-Dimethyl-moφholin-4-yl)-(l-{4-[2- methoxymethyl-7-(3-trifluoromethyl-pyridin-2-yl)-pyrido[2,3-d]pyrimidin-4-ylamino]- phenyl} -cyclobutyl)-methanone; (4-Cyclohexyl-phenyl)-[2-methoxymethyl-7-(3- trifluoromethyl-pyridin-2-yl)-pyrido[3,2-d]pyrimidin-4-yl]-amine; (4-Cyclopentyl-phenyl)-

[2-methoxymethyl-7-(3-trifluoromethyl-pyridin-2-yl)-pyrido[2,3-d]pyrimidin-4-yl]-amine;

(4-Cyclopropyl-phenyl)-[2-methoxymethyl-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4- yl]-amine; (4-Ethyl-phenyl)-[2-methoxymethyl-7-(3-trifluoromethyl-pyridin-2-yl)- pyrido[3,2-d]pyrimidin-4-yl]-amine; (4-Isopropyl-phenyl)-[2-(2-methoxy-ethyl)-7-(3- trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-amine; (4-Isopropyl-phenyl)-[2-(tetrahydro- pyran-4-yloxymethyl)-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-amine; (4- Isopropyl-phenyl)-[2-methoxymethyl-7-(3-methyl-pyridin-2-yl)-pyrido[2,3-d]pyrimidin-4- yl]-amine; (4-Isopropyl-phenyl)-[2-methoxymethyl-7-(3-methyl-pyridin-2-yl)-quinazolin- 4yl]-amine; (4-Isopropyl-phenyl)-[2-methoxymethyl-7-(3-trifluoromethyl-pyridin-2-yl)- pyrido[3,2-d]pyrimidin-4-yl]-amine; (4-Isopropyl-phenyl)-[2-methoxymethyl-7-(3- trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl] -amine; (4-Isopropyl-phenyl)- [2- methoxymethyl-7-(3-trifluoromethyl-pyridin-2-yl)-pyrido[2,3-d]pyrimidin-4-yl]-amine; (4- Isopropyl-phenyl)-[7-(3-methyl-pyridin-2-yl)-2-(tetrahydro-pyran-4-yloxymethyl)- quinazolin-4-yl]-amine; (4-Methanesulfonyl-phenyl)-[2-methoxymethyl-7-(3- trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-amine; (4-Methanesulfonyl-phenyl)-[2- methoxymethyl-7-(3 -trifluoromethyl-pyridin-2-yl)-pyrido [2,3 -d]pyrimidin-4-yl] -amine; (4- _^,ec-Butyl-phenyl)-[2-methoxymethyl-7-(3-methyl-pyridin-2-yl)-pyrido[2,3-d]pyrimidin-4- yl]-amine; (4-5^,ec-Butyl-phenyl)-[2-methoxymethyl-7-(3-trifluoromethyl-pyridin-2-yl)- pyrido[2,3-d]pyrimidin-4-yl]-amine; (4-_-ec-Butyl-phenyl)-[7-(3-chloro-pyridin-2-yl)-2-(2- methoxy-ethoxymethyl)-pyrido[2,3-d]pyrimidin-4-yl]-amine; (4-_'ec-Butyl-phenyl)-[7-(3- chloro-pyridin-2-yl)-2-methoxymethyl-pyrido[2,3-d]pyrimidin-4-yl]-amine; (4-tert-Butyl- phenyl)-[2-(2-methoxy-ethyl)-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-amine; (4- tert-Butyl-phenyl)-[2-(3-diethylamino-l-methyl-propoxymethyl)-7-(3-trifluoromethyl- pyridin-2-yl)-quinazolin-4-yl]-amine; (4-tert-Butyl-phenyl)-[2-(3-diethylamino-l-methyl- propoxymethyl)-7-(3 -trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl] -amine; (4-tert-Butyl- phenyl)-[2-(3-diethylamino-propoxymethyl)-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4- yl]-amine; (4-tert-Butyl-phenyl)-[2-(3-dimethylamino-propoxymethyl)-7-(3-trifluoromethyl- pyridin-2-yl)-quinazolin-4-yl]-amine; (4-tert-Butyl-phenyl)-[2-(3-moφholin-4-yl- propoxymethyl)-7-(3 -trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl] -amine; (4-tert-Butyl- phenyl)-[2-(4-dimethylamino-butoxymethyl)-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin- 4-yl] -amine; (4-tert-Butyl-phenyl)- [2-(4-moφholin-4-yl-butoxymethyl)-7-(3 -trifluoromethyl- pyridin-2-yl)-quinazolin-4-yl]-amine; (4-tert-Butyl-phenyl)-[2-isobutoxymethyl-7-(3-methyl- pyridin-2-yl)-pyrido[2,3-d]pyrimidin-4-yl]-amine; (4-tert-Butyl-phenyl)-[2-isobutoxymethyl- 7-(3-tnfluoromethyl-pyridin-2-yl)-pyrido[2,3-d]pyrimidin-4-yl]-amine; (4-tert-Butyl- phenyl)-[2-methoxymethyl-7-(3-methyl-pyridin-2-yl)-pyrido[2,3-d]pyrimidin-4-yl]-amine; (4-tert-Butyl-phenyl)-[2-methoxymethyl-7-(3-tri.fluoromethyl-pyridin-2-yl)-pyrido[3,2- d]pyrimidin-4-yl]-amine; (4-tert-Butyl-phenyl)-[2-methoxymethyl-7-(3-trifluoromethyl- pyridin-2-yl)-quinazolin-4-yl]-amine; (4-tert-Butyl-phenyl)-[2-methoxymethyl-7-(3- trifluoromethyl-pyridin-2-yl)-pyrido[2,3-d]pyrimidin-4-yl]-amine; (4-tert-Butyl-phenyl)-[7- (3-chloro-pyridin-2-yl)-2-(2-methoxy-ethoxymethyl)-pyrido[2,3-d]pyrimidin-4-yl]-amine; (4- tert-Butyl-phenyl)-[7-(3-chloiO-pyridin-2-yl)-2-ethoxymethyl-pyrido[2,3-d]pyrimidin-4-yl]- amine; (4-tert-Butyl-phenyl)-[7-(3-chloro-pyridin-2-yl)-2-ethoxymethyl-quinazolin-4-yl]- amine; (4-tert-Butyl-phenyl)-[7-(3-chloro-pyridin-2-yl)-2-methoxymethyl-pyrido[2,3- d]pyrimidin-4-yl] -amine; (4-tert-Butyl-phenyl)-[7-(3-chloro-pyridin-2-yl)-2-methoxymethyl- pyrido [3, 2-d]pyrimidin-4-yl] -amine; (4-tert-Butyl-phenyl)-[7-(3-chloro-pyridin-2-yl)-2- methoxymethyl-quinazolin-4-yl]-amine; (6-tert-Butyl-pyridin-3-yl)-[2-(2-methoxy-ethyl)-7- (3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-amine; (6-tert-Butyl-pyridin-3-yl)-[2- isobutoxymethyl-7-(3-methyl-pyridin-2-yl)-pyrido[2,3-d]pyrimidin-4-yl]-amine; (6-tert- Butyl-pyridin-3-yl)-[2-isobutoxymethyl-7-(3-tfifluoromethyl-pyridin-2-yl)-pyrido[2,3- d]pyrimidin-4-yl] -amine; (6-tert-Butyl-pyridin-3-yl)-[2-methoxymethyl-7-(3-methyl-pyridin- 2-yl)-pyrido[2,3-d]pyrimidin-4-yl]-amine; (6-tert-Butyl-pyridin-3-yl)-[2-methoxymethyl-7- (3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-amine; (6-tert-Butyl-pyridin-3-yl)-[2- methoxymethyl-7-(3-trifluoromethyl-pyridin-2-yl)-pyrido[2,3-d]pyrimidin-4-yl]-amine; (6- tert-Butyl-pyridm-3-yl)-[7-(3-chloro-pyridin-2-yl)-2-ethoxymethyl-quinazolin-4-yl]-amine; (6-tert-Butyl-pyridin-3-yl)-[7-(3-chloro-pyridin-2-yl)-2-isobutoxymethyl-pyrido[2,3- d]pyrimidin-4-yl] -amine; (6-tert-Butyl-pyridin-3-yl)-[7-(3-chloro-pyridin-2-yl)-2- methoxymethyl-quinazolin-4-yl]-amine; (6-tert-Butyl-pyridin-3-yl)-[7-(3-chloro-pyridin-2- yl)-2-methoxymethyl-pyrido[2,3-d]pyrimidin-4-yl]-amine; [2-(l-Methyl-piperidin-4- yloxymethyl)-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(6-trifluoromethyl-pyridin- 3-yl)-amine; [2-(2-Diethylamino-ethoxymethyl)-7-(3-trifluoromethyl-pyridin-2-yl)- quinazolin-4-yl]-(6-trifluoromethyl-pyridin-3-yl)-amine; [2-(2-Dimethylamino- ethoxymethyl)-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(4-trifluoromethyl- phenyl)-amine; [2-(2-Piperidin-l-yl-ethoxymethyl)-7-(3-trifluoromethyl-pyridin-2-yl)- quinazolin-4-yl]-(6-trifluoromethyl-pyridin-3-yl)-amine; [2-(3-Benzyloxy-propyl)-7-(3- trifluoromethyl-pyridin-2 -yl)-quinazolin-4-yl] -(4-trifluoromethyl-phenyl)-amine; [2-(3 - Benzyloxy-propyl)-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(6-trifluoromethyl- pyridin-3 -yl)-amine; [2-(3-Benzyloxy-propyl)-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin- 4-yl]-(6-tert-butyl-pyridin-3-yl)-amine; [2-(3-Diethylamino-propoxymethyl)-7-(3- trifluoromethyl-pyridin-2 -yl)-quinazolin-4-yl]-(4-trifluoromethyl-phenyl)-amine; [2-(3- Dimethylamino-2,2-dimethyl-propoxymethyl)-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin- 4-yl]-(4-trifluoromethyl-phenyl)-amine; [2-(3-Dimethylamino-propoxymethyl)-7-(3- trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(4-trifluoromethyl-phenyl)-amine; [2-(Pyridin- 3-ylmethoxymethyl)-7-(3-1rifluoromethyl-pyridin-2-yl)-quinazohn-4-yl]-(6-trifluoromethyl- pyridin-3-yl)-amine; [2-(Pyridin-4-ylmethoxymethyl)-7-(3-trifluoromethyl-pyridin-2-yl)- quinazolin-4-yl]-(6-trifluoromethyl-pyridin-3-yl)-amine; [2-(Tetrahydro-pyran-4- yloxymethyl)-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(4-trifluoiOmethyl-phenyl)- amine; [2-(Tetrahyαjo-pyran-4-yloxymethyl)-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin- 4-yl]-(6-trifluoromethyl-pyridin-3-yl)-amine; [2-Benzyloxymethyl-7-(3-trifluoromethyl- pyridin-2-yl)-quinazolin-4-yl]-[4-(2,2,2-trifluoro-l-methyl-ethyl)-phenyl]-amine; [2- Benzyloxymethyl-7-(3-trifluoromethyl-pyridin-2-yl)-quinazoliιι-4-yl]-(4-trifluoromethyl- phenyl)-amine; [2-Benzyloxymethyl-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(6- trifluoromethyl-pyridin-3-yl)-amine; [2-Benzyloxymethyl-7-(3-trifluoromethyl-pyridin-2-yl)- quinazolin-4-yl]-(5-trifluoromethyl-pyridin-2-yl)-amine; [2-Benzyloxymethyl-7-(3- trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(4-trifluoromethoxy-phenyl)-amine; [2- Benzyloxymethyl-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(4- trifluoromethanesulfonyl-phenyl)-amine; [2-Benzyloxymethyl-7-(3-trifluoromethyl-pyridin- 2-yl)-quinazolin-4-yl]-(4-methanesulfonyl-phenyl)-amine; [2-Benzyloxymethyl-7-(3- trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-[4-(2 -methoxy- 1 , 1 -dimethyl-ethyl)-phenyl]- amine; [2-Benzyloxymethyl-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(4-tert- butyl-phenyl)-amine; [2-Benzyloxymethyl-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4- yl]-( 1 -methanesulfonyl-2,3 -dihydro- 1 H-indol-5-yl)-amine; [2-Cyclopentyloxymethyl-7-(3 - trifluoromethyl-pyridin-2 -yl)-quinazolin-4-yl]-[4-(moφholine-4-sulfonyl)-phenyl]-amine; [2- Cyclopropylmethoxymethyl-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-[4- (moφholine-4-sulfonyl)-phenyl]-amine; [2-Ethoxymethyl-7-(3-methyl-pyridin-2-yl)- pyrido[2,3-d]pyrimidin-4-yl]-(4-trifluoromethyl-phenyl)-amine; [2-Ethoxymethyl-7-(3- methyl-pyridin-2-yl)-pyrido[2,3-d]pyrimidin-4-yl]-(4-isopropyl-phenyl)-amine; [2- Ethoxymethyl-7-(3-methyl-pyridin-2-yl)-pyrido[2,3-d]pyrimidin-4-yl]-(4- trifluoromethanesulfonyl-phenyl)-amine; [2-Ethoxymethyl-7-(3-methyl-pyridin-2-yl)- quinazolin-4-yl]-(4-isopropyl-phenyl)-amine; [2-Ethoxymethyl-7-(3-methyl-pyridin-2-yl)- quinazolin-4-yl] -(4-trifluoromethyl-phenyl)-amine; [2-Ethoxymethyl-7-(3 -trifluoromethyl- pyridin-2-yl)-pyrido[2,3-d]pyrimidin-4-yl]-(4-trifluoromethyl-phenyl)-amine; [2- Ethoxymethyl-7-(3-trifluoromethyl-pyridin-2-yl)-pyrido[2,3-d]pyrimidin-4-yl]-(6- trifluoromethyl-pyridin-3-yl)-amine; [2-Ethoxymethyl-7-(3-trifluoromethyl-pyridin-2-yl)- quinazolin-4-yl] -(4-trifluoromethyl-phenyl)-amine; [2-Ethoxymethyl-7-(3 -trifluoromethyl- pyridin-2-yl)-quinazolin-4-yl] -[4-(moφholine-4-sulfonyl)-phenyl] -amine; [2-Ethoxymethyl- 7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(6-trifluoromethyl-pyridin-3-yl)-amine; [2-Isobutoxymethyl-7-(3-methyl-pyridin-2-yl)-pyrido[2,3-d]pyrimidin-4-yl]-(4-isopropyl- phenyl)-amine; [2-Isobutoxymethyl-7-(3-methyl-pyridin-2-yl)-pyrido[2,3-d]pyrimidin-4-yl]- (4-trifluoromethyl-phenyl)-amine; [2-Isobutoxymethyl-7-(3-methyl-pyridin-2-yl)-pyrido[2,3- d]pyrimidin-4-yl]-(3-methyl-4-trifluoromethyl-phenyl)-amine; [2-Isobutoxymethyl-7-(3- methyl-pyridin-2-yl)-pyrido[2,3-d]pyrimidin-4-yl]-(4-trifluoromethoxy-phenyl)-amine; [2- Isobutoxymethyl-7-(3 -methyl-pyridin-2-yl)-pyrido [2,3 -d]pyrimidin-4-yl] - [4-(2-methoxy- 1,1- dimethyl-ethyl)-phenyl]-amine; [2-Isobutoxymethyl-7-(3-methyl-pyridin-2-yl)-pyrido[2,3- d]pyrimidin-4-yl]-(4-methanesulfonyl-phenyl)-amine; [2-Isobutoxymethyl-7-(3- trifluoromethyl-pyridin-2-yl)-pyrido[2,3-d]pyrimidin-4-yl]-(4-isopropyl-phenyl)-amine; [2- Isobutoxymethyl-7-(3-trifluoromethyl-pyridin-2-yl)-pyrido[2,3-d]pyrimidin-4-yl]-(4- trifluoromethyl-phenyl)-amine; [2-Isobutoxymethyl-7-(3-trifluoromethyl-pyridin-2-yl)- pyrido[2,3-d]pyrimidin-4-yl]-(6-trifluoromethyl-pyridin-3-yl)-amine; [2-Isobutoxymethyl-7- (3-trifluoromethyl-pyridin-2-yl)-pyrido[2,3-d]pyrimidin-4-yl]-(3-methyl-4-trifluoromethyl- phenyl)-amine; [2-Isobutoxymethyl-7-(3-trifluoromethyl-pyridin-2-yl)-pyrido[2,3- d]pyrimidin-4-yl]-(4-trifluoromethanesulfonyl-phenyl)-amine; [2-Isobutoxymethyl-7-(3- trifluoromethyl-pyridin-2-yl)-pyrido[2,3-d]pyrimidin-4-yl]-(4-trifluoromethoxy-phenyl)- amine; [2-Isobutoxymethyl-7-(3-trifluoromethyl-pyridin-2-yl)-pyrido[2,3-d]pyrimidin-4-yl]- (4-methanesulfonyl-phenyl)-amine; [2-Isopropoxymethyl-7-(3-methyl-pyridin-2-yl)- quinazolin-4-yl] -(4-trifluoromethyl-phenyl)-amine; [2-Isopropoxymethyl-7-(3 -methyl- pyridin-2-yl)-quinazolin-4-yl] -(4-isopropyl-phenyl)-amine; [2-Isopropoxymethyl-7-(3 - trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-[4-(moφholine-4-sulfonyl)-phenyl]-amine; [2- Isopropoxymethyl-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(6-trifluoromethyl- pyridin-3 -yl)-amine; [2-Methoxymethyl-7-(3-methyl-pyridin-2-yl)-pyrido[2,3-d]pyrimidin-4- yl]-(4-trifluoromethyl-phenyl)-amine; [2-Methoxymethyl-7-(3-methyl-pyridin-2-yl)- pyrido[2,3-d]pyrimidin-4-yl]-(6-trifluoromethyl-pyridin-3-yl)-amine; [2-Methoxymethyl-7- (3-methyl-pyridin-2-yl)-pyrido[2,3-d]pyrimidin-4-yl]-(4-trifluoromethanesulfonyl-phenyl)- amine; [2-Methoxymethyl-7-(3-methyl-pyridin-2-yl)-pyrido[2,3-d]pyrimidin-4-yl]-(4- trifluoromethoxy-phenyl)-amine; [2-Methoxymethyl-7-(3-methyl-pyridin-2-yl)-pyrido[2,3- d]pyrimidin-4-yl]-(3-methyl-4-trifluoromethyl-phenyl)-amine; [2-Methoxymethyl-7-(3- trifluoromethyl-pyridin-2-yl)-pyrido[2,3-d]pyrimidin-4-yl]-[4-(2,2,2-trifluoro-l-methyl- ethyl)-phenyl]-amine; [2-Methoxymethyl-7-(3-trifluoromethyl-pyridin-2-yl)-pyrido[2,3- d]pyrimidin-4-yl]-(4-trifluoromethyl-phenyl)-amine; [2-Methoxymethyl-7-(3- trifluoromethyl-pyridin-2-yl)-pyrido[2,3-d]pyrimidin-4-yl]-(4-trifluoromethanesulfonyl- phenyl)-amine; [2-Methoxymethyl-7-(3-trifluoromethyl-pyridin-2-yl)-pyrido[2,3- d]pyrimidin-4-yl]-[4-(moφholine-4-sulfonyl)-phenyl]-amine; [2-Methoxymethyl-7-(3- trifluoromethyl-pyridin-2-yl)-pyrido[2,3-d]pyrimidin-4-yl]-(6-trifluoromethyl-pyridin-3-yl)- amine; [2-Methoxymethyl-7-(3-trifluoromethyl-pyridin-2-yl)-pyrido[2,3-d]pyrimidin-4-yl]- (3 -methyl-4-trifluoromethyl-phenyl)-amine; [2-Methoxymethyl-7-(3 -trifluoromethyl-pyridin- 2-yl)-pyrido[2,3-d]pyrimidin-4-yl]-(4-trifluoromethoxy-phenyl)-amine; [2-Methoxymethyl-7- (3-trifluoromethyl-pyridin-2-yl)-pyrido[3,2-d]pyrimidin-4-yl]-(6-trifluoromethyl-pyridin-3- yl)-amine; [2-Methoxymethyl-7-(3-trifluoromethyl-pyridin-2-yl)-pyrido[3,2-d]pyrimidin-4- yl]-(4-moφholin-4-yl-phenyl)-amine; [2-Methoxymethyl-7-(3-trifluoromethyl-pyridin-2-yl)- quinazolin-4-yl]-(4-trifluoromethyl-phenyl)-amine; [2-Methoxymethyl-7-(3-trifluoromethyl- pyridin-2-yl)-quinazolin-4-yl] -(6-trifluoromethyl-p yridin-3 -yl)-amine; [2-Methoxymethyl-7- (3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(4-trifluoromethanesulfonyl-phenyl)-amine; [2-Methoxymethyl-7-(3-tnfluoromethyl-pyridin-2-yl)-quinazolin-4-yl]-(5-trifluoromethyl- pyridin-2-yl)-amine; [2-Methoxymethyl-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-yl]- [4-(moφholine-4-sulfonyl)-phenyl]-amine; [4-(2 -Diethylamino- 1 , 1 -dimethyl-ethyl)-phenyl]- [2-methoxyinethyl-7-(3-methyl-pyridin-2-yl)-pyrido[2,3-d]pyrimidin-4-yl]-amine; [4-(2- Methoxy- 1 , 1 -dimethyl-ethyl)-phenyl] -[2-methoxymethyl-7-(3 -trifluoromethyl-pyridin-2 -yl)- quinazolin-4-yl] -amine; [4-(2-Methoxy-l,l-dimethyl-ethyl)-phenyl]-[2-methoxymethyl-7-(3- trifluoromethyl-pyridin-2-yl)-pyrido [3,2-d]pyrimidin-4-yl]-amine; [4-(4-Isopropyl- phenylamino)-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-2-yl]-methanol; [4-(4-tert-Butyl- phenylamino)-7-(3-chloro-pyridin-2-yl)-quinazolin-2-yl]-methanol; [4-(4-tert-Butyl- phenylamino)-7-(3-methyl-pyridin-2-yl)-pyrido[2,3d]pyrimidin-2-yl]-methanol; [4-(4-tert- Butyl-phenylamino)-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-2-yl]-methanol; [4-(4-tert- Butyl-phenylamino)-7-(3-trifluoromethyl-pyridin-2-yl)-pyrido[3,2-d]pyrimidin-2-yl]- methanol; [4-(4-Trifluoromethyl-phenylamino)-7-(3 -trifluoromethyl-pyridin-2-yl)- quinazolin-2-yl] -methanol; [4-(4-Trifluoromethyl-phenylamino)-7-(3-trifluoromethyl- pyridin-2-yl)-pyrido [3 ,2-d]pyrimidin-2-yl] -methanol; [4-(4-Trifluoromethyl-phenylamino)-7- (3-trifluoromethyl-pyridin-2-yl)-pyrido[2,3-d]pyrimidin-2-yl]-methanol; [4-(Moφholine-4- sulfonyl)-phenyl]-[2-(tetrahydro-pyran-4-yloxymethyl)-7-(3-trifluoromethyl-pyridin-2-yl)- qumazolin-4-yl]-amine; [4-[4-(Piperidine-l-sulfonyl)-phenylamino]-7-(3-trifluoromethyl- pyridin-2-yl)-quinazolin-2-yl]-methanol; [4-[4-(Piperidine-l-sulfonyl)-phenylamino]-7-(3- trifluoromethyl-pyridin-2-yl)-pyrido[3,2-d]pyrimidin-2-yl]-methanol; [7-(3-Chloro-pyridin-2- yl)-2-(2-methoxy-ethoxymethyl)-pyrido[2,3-d]pyrimidin-4-yl]-(4-trifluorometliyl-phenyl)- amine; [7-(3-Chloro-pyridin-2-yl)-2-(2-methoxy-ethoxymethyl)-pyrido[2,3-d]pyrimidin-4- yl]-(4-isopropyl-phenyl)-amine; [7-(3-Chloro-pyridin-2-yl)-2-(2-methoxy-ethyl)-pyrido[2,3- d]pyrimidin-4-yl]-(4-trifluoromethyl-phenyl)-amine; [7-(3-Chloro-pyridm-2-yl)-2- (tetrahydro-pyran-4-yloxymethyl)-quinazolin-4-yl]-(4-trifluoromethyl-phenyl)-amine; [7-(3- Chloro-pyridin-2-yl)-2-(tetrahydro-pyran-4-yloxymethyl)-quinazolin-4-yl]-(4-isopropyl- phenyl)-amine; [7-(3-Chloro-pyridin-2-yl)-2-ethoxymethyl-pyrido[2,3-d]pyrimidin-4-yl]-(4- isopropyl-phenyl)-amine; [7-(3-Chloro-pyridin-2-yl)-2-ethoxymethyl-pyrido[2,3- d]pyrimidin-4-yl]-(4-trifluoromethyl-phenyl)-amine; [7-(3-Chloro-pyridin-2-yl)-2- ethoxymethyl-pyrido[2,3-d]pyrimidin-4-yl]-(4-trifluoromethanesulfonyl-phenyl)-amine; [7- (3-Chloro-pyridin-2-yl)-2-ethoxymethyl-pyrido[2,3-d]pyrimidin-4-yl]-[4-(2,2,2-trifluoro- 1 - methyl-efhyl)-phenyl] -amine; [7-(3-Chloro-pyridin-2-yl)-2-ethoxymethyl-pyrido[2,3- d]pyrimidin-4-yl]-(4-cyclopentyl-phenyl)-amine; [7-(3-Chloro-pyridin-2-yl)-2-ethoxymethyl- pyrido[2,3-d]pyrimidin-4-yl]-(6-trifluoromethyl-pyridin-3-yl)-amine; [7-(3-Chloro-pyridin-2- yl)-2-ethoxymethyl-pyrido[2,3-d]pyrimidin-4-yl]-(3-methyl-4-trifluoromethyl-phenyl)- amine; [7-(3-Chloro-pyridin-2-yl)-2-ethoxymethyl-quinazolin-4-yl]-(4-isopropyl-phenyl)- amine; [7-(3-Chloro-pyridin-2-yl)-2-ethoxvmethyl-quinazolin-4-yl]-(4-trifluoromethyl- phenyl)-amine; [7-(3-Chloro-pyridin-2-yl)-2-isobutoxymethyl-pyrido[2,3-d]pyrimidin-4-yl]- (4-trifluoromethyl-phenyl)-amine; [7-(3 -Chloro-pyridin-2-yl)-2-isobutoxymethyl-pyrido [2,3 - d]pyrimidin-4-yl]-(6-trifluoromethyl-pyridin-3-yl)-amine; [7-(3-Chloro-pyridin-2-yl)-2- isobutoxymethyl-pyrido[2,3-d]pyrimidin-4-yl]-(3-methyl-4-trifluoromethyl-phenyl)-amine; [7-(3-Chloro-pyridin-2-yl)-2-isobutoxymethyl-pyrido[2,3-d]pyrimidin-4-yl]-(4- trifluoromethoxy-phenyl)-amine; [7-(3-Chloro-pyridin-2-yl)-2-isobutoxymethyl-pyrido[2,3- d]pyrimidin-4-yl] -(4-trifluoromethanesulfonyl-phenyl)-amine; [7-(3 -Chloro-pyridin-2-yl)-2- isobutoxymethyl-pyrido[2,3-d]pyrimidin-4-yl]-(4-methanesulfonyl-phenyl)-amine; [7-(3- Chloro-pyridin-2-yl)-2-methoxymethyl-pyrido[2,3-d]pyrimidin-4-yl]-(4-isopropyl-phenyl)- amine; [7-(3-Chloro-pyridin-2-yl)-2-methoxymethyl-pyrido[2,3-d]pyrimidin-4-yl]-[4-(2- methoxy- 1 , 1 -dimethyl-ethyl)-phenyl] -amine; [7-(3-Chloro-pyridin-2-yl)-2-methoxymethyl- pyrido[2,3-d]pyrimidin-4-yl]-(4-trifluoromethoxy-phenyl)-amine; [7-(3-Chloro-ρyridin-2-yl)- 2-methoxymethyl-pyrido[2,3-d]pyrimidin-4-yl]-(3-methyl-4-trifluoromethyl-phenyl)-amine; [7-(3-Chloro-pyridin-2-yl)-2-methoxymethyl-pyrido[3,2-d]pyrimidin-4-yl]-(4- trifluoromethyl-phenyl)-amine; [7-(3-Chloro-pyridin-2-yl)-2-methoxymethyl-pyrido[3,2- d]pyrimidin-4-yl]-(4-isopropyl-phenyl)-amine; [7-(3-Chloro-pyridin-2-yl)-2-methoxymethyl- pyrido[3,2-d]pyrimidin-4-yl]-[4-(moφholine-4-sulfonyl)-phenyl]-amine; [7-(3-Chloro- pyridin-2-yl)-2-methoxymethyl-quinazolin-4-yl] -(4-isopropyl-phenyl)-amine; [7-(3 -Chloro- pyridin-2-yl)-2-methoxymethyl-quinazolin-4-yl]-(4-trifluoromethyl-phenyl)-amine; [7-(3- Chloro-pyridin-2-yl)-4-(4-isopropyl-phenylamino)-quinazolin-2-yl]-methanol; [7-(3-Chloro- pyridin-2-yl)-4-(4-tfifluoromethyl-phenylamino)-pyrido[3,2-d]pyrimidin-2-yl]-methanol; [7- (3-Methyl-pyridin-2-yl)-2-(tetrahydro-furan-3-yl)-pyrido[2,3-d]pyrimidin-4-yl]-(4- trifluoromethyl-phenyl)-amine; [7-(3-Methyl-pyridin-2-yl)-2-(tetrahydro-pyran-4- yloxymethyl)-quinazolin-4-yl]-(4-trifluoromethyl-phenyl)-amine; [7-(3-Methyl-pyridin-2-yl)- 4-(4-trifluoromethyl-phenylamino)-pyrido[2,3-d]pyrimidin-2-yl]-methanol; [7-(3- Trifluoromethyl-pyridin-2-yl)-4-(6-trifluoromethyl-pyridin-3-ylamino)-quinazolin-2-yl]- methanol; l-{4-[2-Isobutoxymethyl-7-(3-methyl-pyridin-2-yl)-pyrido[2,3-d]pyrimidin-4- ylamino]-phenyl}-cyclobutanecarbonitrile; l-{4-[2-Methoxymethyl-7-(3-methyl-pyridin-2- yl)-pyrido[2,3-d]pyrimidin-4-ylamino]-phenyl}-cyclobutanecarbonitrile; l-{4-[2- Methoxymethyl-7-(3-trifluoromethyl-pyridin-2-yl)-pyrido[3,2-d]pyrimidin-4-ylamino]- phenyl} -ethanone; l-{4-[2-Methoxymethyl-7-(3-trifluoromethyl-pyridin-2-yl)-pyrido[3,2- d]pyrimidin-4-ylamino]-phenyl}-butan-l-one; l-{4-[7-(3-Chloro-pyridin-2-yl)-2- ethoxymethyl-pyrido[2,3-d]pyrimidin-4-ylamino]-phenyl}-cyclobutanecarbonitrile; 1- Dimethylamino-3-[4-(4-trifluoromethyl-phenylamino)-7-(3-trifluoromethyl-pyridin-2-yl)- quinazolin-2-ylmethoxy]-propan-2-ol; 2-[4-(4-tert-Butyl-phenylamino)-7-(3-trifluoromethyl- pyridin-2-yl)-quinazolin-2-yl] -2-methyl-propan- 1 -ol; 2- {4- [2-Benzyloxymethyl-7-(3 - trifluoromethyl-pyridin-2-yl)-quinazolin-4-ylamino]-phenyl}-2-methyl-propionitrile; 2- {4-[2- Ethoxymethyl-7-(3-trifluoromethyl-pyridin-2-yl)-pyrido[2,3-d]pyrimidin-4-ylamino]- phenyl} -2-methyl-propionitrile; 2- {4-[2-Isobutoxymethyl-7-(3-methyl-pyridin-2-yl)- pyrido[2,3-d]pyrimidin-4-ylamino]-phenyl}-2 -methyl-propionitrile; 2-{4-[2- Isobutoxymethyl-7-(3-trifluoromethyl-pyridin-2-yl)-pyrido[2,3-d]pyrimidin-4-ylamino]- phenyl} -2-methyl-propionitrile; 2-{4-[2-Methoxymethyl-7-(3-methyl-pyridin-2-yl)- pyrido[2,3-d]pyrimidin-4-ylamino]-phenyl}-2-methyl-propionitrile; 2-{4-[2-Methoxymethyl- 7-(3-1rifluoromethyl-pyridin-2-yl)-quinazolin-4-ylamino]-phenyl}-2-methyl-propionitrile; 2- {4-[7-(3-Chloro-pyridin-2-yl)-2-(2-methoxy-ethoxymethyl)-pyrido[2,3-d]pyrimidin-4- ylamino]-phenyl} -2-methyl-propionitrile; 2- {4-[7-(3-Chloro-pyridin-2-yl)-2- isobutoxymethyl-pyrido [2,3 -d]pyrimidin-4-ylamino] -phenyl} -2-methyl-propionitrile; 2- {4- [7-(3-Chloro-pyridin-2-yl)-2-methoxymethyl-pyrido[2,3-d]pyrimidin-4-ylamino]-phenyl}-2- methyl-propionitrile; 2-Methyl-2-[4-(4-trifluoromethyl-phenylamino)-7-(3-trifluoromethyl- pyridin-2-yl)-quinazolin-2-yl]-propan-l-ol; 2-Methyl-2-{4-[7-(3-methyl-pyridin-2-yl)-2- (tetrahydro-ftiran-3-yl)-pyrido[2,3-d]pyrimidin-4-ylamino]-phenyl}-propionitrile; 3-[4-(6- tert-Butyl-pyridin-3-ylamino)-7-(3-trifluoroιnethyl-pyridin-2-yl)-quinazolin-2-yl]-propan-l- ol; 3-[7-(3-Trifluoromethyl-pyridin-2-yl)-4-(6-trifluoromethyl-pyridin-3-ylamino)- quinazolin-2-yl]-proρan-l-ol; 3-{4-[2-Isobutoxymethyl-7-(3-methyl-pyridin-2-yl)- pyrido [2,3 -d]pyrimidin-4-ylamino] -phenyl} -3-methyl-butan-2-one; 3- {4-[2-

Isobutoxymethyl-7-(3-trifluororhethyl-pyridin-2-yl)-pyrido[2,3-d]pyrimidin-4-ylamino]- phenyl}-3-methyl-butan-2-one; 3-{4-[2-Methoxymethyl-7-(3-methyl-pyridin-2-yl)- pyrido[2,3-d]pyrimidm-4-ylamino]-phenyl}-3-methyl-butan-2-one; 3-{4-[2-Methoxymethyl- 7-(3-trifluoromethyl-pyridin-2-yl)-pyrido[2,3-d]pyrimidin-4-ylamino]-phenyl}-3-methyl- butan-2-one; 3- {4-[7-(3-Chloro-pyridin-2-yl)-2-isobutoxymethyl-pyrido[2,3-d]pyrimidin-4- ylamino]-phenyl}-3-methyl-butan-2-one; 3-{4-[7-(3-Chloro-pyridin-2-yl)-2-methoxymethyl- pyrido[2,3-d]pyrimidin-4-ylamino]-phenyl}-3-methyl-butan-2-one; 4-[2-Benzyloxymethyl-7- (3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-ylamino]-N-tert-butyl-benzenesulfonamide; 4- [2-Methoxymethyl-7-(3 -trifluoromethyl-pyridin-2-yl)-pyrido [3 ,2-d]pyrimidin-4-ylamino] - benzonitrile; N,N-Diethyl-2- {4-[2-isobutoxymethyl-7-(3-methyl-pyridin-2-yl)-pyrido[2,3- d]pyrimidin-4-ylamino]-phenyl}-isobutyr amide; N,N-Diethyl-2-{4-[2-methoxymethyl-7-(3- trifluoromethyl-pyridin-2-yl)-pyrido[2,3-d]pyrimidin-4-ylamino]-phenyl}-isobutyramide; N- tert-Butyl-4-[2-hydroxymethyl-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-ylamino]-N- methyl-benzenesulfonamide; N-tert-Butyl-4-[2-hydroxymethyl-7-(3-trifluoromethyl-pyridin- 2-yl)-pyrido[3,2-d]pyrimidin-4-ylamino]-N-methyl-benzenesulfonamide; and N-tert-Butyl-4- [2-methoxymethyl-7-(3-trifluoromethyl-pyridin-2-yl)-quinazolin-4-ylamino]- benzenesulfonamide.

78. A compound or salt according to any one of claims 1, 16, 34, or 61, wherein the compound has an IC₅₀ value of 100 nanomolar or less in a capsaicin receptor calcium mobilization assay.

79. A compound or salt according to any one of claims 1, 16, 34, or 61, wherein the compound has an IC₅₀ value of 10 nanomolar or less in a capsaicin receptor calcium mobilization assay.

80. A compound or salt according to any one of claims 1, 16, 34, or 61, wherein the compound has an IC₅₀ value of 1 nanomolar or less in a capsaicin receptor calcium mobilization assay.

81. A pharmaceutical composition, comprising at least one compound or salt according to any one of claims 1, 16, 34, or 61 in combination with a physiologically acceptable carrier or excipient.

82. A pharmaceutical composition according to claim 81, wherein the composition is formulated as an injectible fluid, an aerosol, a cream, a gel, a pill, a capsule, a syrup or a transdermal patch.

83. A method for reducing calcium conductance of a cellular capsaicin receptor, comprising contacting a cell expressing a capsaicin receptor with at least one compound or salt ofthe formula:

wherein:

V, X, W,Y and Z are each independently N or CR_l5 with the proviso that at least one of V and X is N; U is N or CR₂, with the proviso that if V and X are N, then U is CR₂; Ri is independently selected at each occunence from hydrogen, halogen, hydroxy, cyano, amino, d-C₈alkyl, haloCi-C₈alkyl, Cι-C₈alkoxy, halod-C₈alkoxy and mono- and di-(d-

C₈alkyl)amino; R is: (i) hydrogen, halogen, cyano or -COOH;

(ii) Ci-C₈alkanoyl, C_-C₈alkanone, or Cι-C₈carbamate, each of which is unsubstituted or substituted with from 1 to 9 substituents independently selected from R_b; or (iii) a group ofthe formula -R_c-M-A-R_y, wherein: R_e is C₀-C₃alkyl;

M is a bond, N(R_Z), O, S, SO₂, -N(R_Z)-, (C=0)_pN(R_z), N(R_z)(C=O)_p, SO₂N(R_z), or N(R_z)SO₂, wherein p is 0 or 1; A is a bond or d-C₈alkyl, optionally substituted with from 1 to 3 substituents independently selected from R_b; and R_y and R_z are:

(a) independently hydrogen, Ci-C₈alkyl, C₂-C₈alkenyl, C₂-C₈ alkynyl, C₆- Ci₀arylCι-C₈alkyl, C -C₈alkyl ether, Ci-C₈alkoxy, a 4- to 10- membered carbocycle or heterocycle, or joined to R_e to form a 4- to

10-membered carbocycle or heterocycle, wherein each R_y and R_z is independently unsubstituted or substituted with from 1 to 9 substituents independently selected from R ; or

(b) joined to form a 4- to 10-membered heterocycle that is unsubstituted or substituted with from 1 to 9 substituents independently selected from

Ari and Ar₂ are independently selected from 5- to 10-membered aromatic carbocycles and heterocycles, each of which is unsubstituted or substituted with from 1 to 3 substituents independently selected from groups ofthe formula LR_a; L is independently selected at each occunence from a bond, -O-, -C(=O)-, -OC(=O)-, -C(=O)O-, -0-C(=O)O-, -S(O)_m-, -NR_X-, -C(-O)NHR_x-, -NHR_xC(=O)-, -NR_xS(O)_m-, -S(O)_mNR_x- and -N[S(O)_mR_x]S(O)_m-; wherein m is independently selected at each occunence from 0, 1 and 2; and R_x is independently selected at each occunence from hydrogen and d-C₈alkyl; R_a is independently selected at each occunence from: (i) hydrogen, halogen, cyano and nitro; and

(ii) Cι-C₈alkyl, C₂-C₈alkenyl, C₂-C₈alkynyl, C -C₈alkyl ether, 3- to 10-membered heterocycles, mono- and di-(Ci-C₈alkyl)amino and (3- to 10-membered heterocycle)d- C₆alkyl, each of which is unsubstituted or substituted with firom 1 to 9 substituents independently selected from R ; and

R_b is independently chosen at each occunence from hydroxy, halogen, amino, aminocarbonyl, amido, cyano, nitro, Ci-C₈alkyl, d-C₈alkoxy, d-C₈alkylthio, Ci-C₈alkyl ether, hydroxyd-C.alkyl, halod-C₈alkyl, phenyl, phenyl(d-C₈alkyl), mono-and di-(d- C₆alkyl)amino, (SO₂)Cι-C₈alkyl, 5- to 7-membered heterocycle and (5- to 7-membered heterocycle)(C ι -C₈alkyl) ; and thereby reducing calcium conductance ofthe capsaicin receptor.

84. A method according to claim 83, wherein the compound is a compound according to any one of claims 1, 16, 34, or 61.

85. A method according to claim 83, wherein the cell is a neuronal cell that is contacted in vivo in an animal.

86. A method according to claim 85, wherein during contact the compound is present within a body fluid ofthe animal.

87. A method according to claim 86, wherein the compound is present in the blood of the animal at a concentration of 100 nanomolar or less.

88. A method according to claim 85, wherein the animal is a human.

89. A method according to claim 85, wherein the compound is administered orally.

90. A method for inhibiting binding of vanilloid ligand to a capsaicin receptor in vitro, the method comprising contacting capsaicin receptor with at least one compound or salt ofthe formula:

HN^'Ar2

Ari Λ_ZA_V wherein:

V, X, W,Y and Z are each independently N or CR_l5 with the proviso that at least one of V and

X is N; U is N or CR₂, with the proviso that if V and X are N, then U is CR₂; Ri is independently selected at each occurrence from hydrogen, halogen, hydroxy, cyano, amino, d-C₈alkyl, haloCι-C₈alkyl, Ci-C₈alkoxy, haloC_.-C_.alkoxy and mono- and di-(d- C₈alkyl)amino; R₂ is: (i) hydrogen, halogen, cyano or -COOH; (ii) Ci-C₈alkanoyl, C₂-C₈alkanone, or Ci-C_.carbamate, each of which is unsubstituted or substituted with from 1 to 9 substituents independently selected (iii) a group ofthe formula -R_c-M-A-R_y, wherein: R_e is C₀-C₃alkyl; M is a bond, N(R_Z), O, S, SO₂, -N(R_Z)-, (C=O)_pN(R_z), N(R_z)(C=O)_p,

S0₂N(R_z), or N(R_z)SO₂, wherein p is 0 or 1; A is a bond or Cι-C₈alkyl, optionally substituted with from 1 to 3 substituents independently selected from R_b; and R_y and R_z are: (a) independently hydrogen, Ci-C₈alkyl, C₂-C₈alkenyl, C₂-C₈ alkynyl, C₆-

CιoarylCi-C₈alkyl, C₂-C₈alkyl ether, d-C₈alkoxy, a 4- to 10- membered carbocycle or heterocycle, or joined to R_e to form a 4- to 10-membered carbocycle or heterocycle, wherein each R_y and R_z is independently unsubstituted or substituted with from 1 to 9 substituents independently selected from R_b; or

(b) joined to form a 4- to 10-membered heterocycle that is unsubstituted or substituted with from 1 to 9 substituents independently selected firom

R_b; Ari and Ar₂ are independently selected from 5- to 10-membered aromatic carbocycles and heterocycles, each of which is unsubstituted or substituted with from 1 to 3 substituents independently selected from groups ofthe formula LR_a; L is independently selected at each occunence from a bond, -O-, -C(=O)-, -OC(=O)-, -C(=O)O-, -O-C(=O)O-, -S(O)_m-, -NR_X-, -C(=O)NHR_x-, -NHR_xC(=O)-, -NR_xS(O)_m-, -S(O)_mNR_x- and -N[S(O)_mR_x]S(O)_m-; wherein m is independently selected at each occunence from 0, 1 and 2; and R_x is independently selected at each occunence from hydrogen and d-C₈alkyl; R_a is independently selected at each occunence from: (i) hydrogen, halogen, cyano and nitro; and (ii) Cι-C₈alkyl, C₂-C₈alkenyl, C₂-C₈alkynyl, C₂-C₈alkyl ether, 3- to 10-membered heterocycles, mono- and di-(Ci-C₈alkyl)amino and (3- to 10-membered heterocycle)C₁- C_.alkyl, each of which is unsubstituted or substituted with from 1 to 9 substituents independently selected from R_b; and R_b is independently chosen at each occunence from hydroxy, halogen, amino, aminocarbonyl, amido, cyano, nitro, d-C₈alkyl, Ci-C₈alkoxy, Cι-C₈alkylthio, d-C₈alkyl ether, hydroxyd-C₈alkyl, haloCi-C_.alkyl, phenyl, phenyl(Cι-C₈alkyl), mono-and di-(C_- C₆alkyl)amino, (SO₂)Ci-C₈alkyl, 5- to 7-membered heterocycle and (5- to 7-membered heterocycle)(Ci-C₈alkyl); under conditions and in an amount sufficient to detectably inhibit vanilloid ligand binding to capsaicin receptor.

91. A method according to claim 90, wherein the compound is a compound according to any one of claims 1, 16, 34, or 61.

92. A method for inhibiting binding of vanilloid ligand to capsaicin receptor in a patient, comprising contacting cells expressing capsaicin receptor with a compound or salt of the formula:

HN'^AP2

wherein:

V, X, W,Y and Z are each independently N or CRi, with the proviso that at least one of V and

X is N; U is N or CR , with the proviso that if V and X are N, then U is CR₂;

Ri is independently selected at each occunence from hydrogen, halogen, hydroxy, cyano, amino, d-C₈alkyl, halod-C₈alkyl, d-C₈alkoxy, halod-C₈alkoxy and mono- and di-(Cι-

C₈alkyl)amino; R₂ is: (i) hydrogen, halogen, cyano or -COOH;

(ii) Ci-C₈alkanoyl, C₂-C₈alkanone, or Ci-C₈carbamate, each of which is unsubstituted or substituted with from 1 to 9 substituents independently selected (iii) a group ofthe formula -R_c-M-A-Ry, wherein: R_e is C₀-C₃ alkyl;

M is a bond, N(R_Z), O, S, SO₂, -N(R_Z)-, (C-O)_pN(R_z), N(R_z)(C=O)_p, SO₂N(R_z), or N(R_z)SO₂, wherein p is 0 or 1 ; A is a bond or Cι-C₈alkyl, optionally substituted with from 1 to 3 substituents independently selected from R ; and R_y and R_z are:

(a) independently hydrogen, Ci-C₈alkyl, C₂-C₈alkenyl, C₂-C₈ alkynyl, C₆- doarylCi-C_.alkyl, C₂-C₈alkyl ether, Cι-C₈alkoxy, a 4- to 10- membered carbocycle or heterocycle, or joined to R_e to form a 4- to

10-membered carbocycle or heterocycle, wherein each R_y and R_z is independently unsubstituted or substituted with from 1 to 9 substituents independently selected from R ; or

(b) joined to form a 4- to 10-membered heterocycle that is unsubstituted or substituted with from 1 to 9 substituents independently selected firom

R_b;

Ai and Ar₂ are independently selected from 5- to 10-membered aromatic carbocycles and heterocycles, each of which is unsubstituted or substituted with from 1 to 3 substituents independently selected from groups ofthe formula LR_a; L is independently selected at each occunence from a bond, -O-, -C(=O)-, -OC(=O)-, -C θ)O-, -0-C(=O)0-, -S(O)_m-, -NR_X-, -C(=O)NHR_x-, -NHR_xC(=O)-, -NR_xS(O)_m-, -S(O)_mNR_x- and -N[S(O)_mR_x]S(O)_m-; wherein m is independently selected at each occunence from 0, 1 and 2; and R_x is independently selected at each occurrence from hydrogen and Ci-C₈alkyl; R_a is independently selected at each occunence from: (i) hydrogen, halogen, cyano and nitro; and

(ii) Ci-C₈alkyl, C₂-C₈alkenyl, C₂-C₈alkynyl, C₂-C₈alkyl ether, 3- to 10-membered heterocycles, mono- and di-(Ci-C_alkyl)amino and (3- to 10-membered heterocycle)d- dalkyl, each of which is unsubstituted or substituted with from 1 to 9 substituents independently selected from R_b; and

R_b is independently chosen at each occunence from hydroxy, halogen, amino, aminocarbonyl, amido, cyano, nitro, d-C₈alkyl, d-C₈alkoxy, Cι-C₈alkylthio, Ci-C₈alkyl ether, hydroxyd-C₈alkyl, haloCι-C₈alkyl, phenyl, phenyl(Cι-C₈alkyl), mono-and di-(d- C₆alkyl)amino, (SO₂)Ci-C₈alkyl, 5- to 7-membered heterocycle and (5- to 7-membered heterocycle)(C i -C₈alkyl) ; in an amount sufficient to detectably inhibit vanilloid ligand binding to cells expressing a cloned capsaicin receptor in vitro, and thereby inhibiting binding of vanilloid ligand to the capsaicin receptor in the patient.

93. A method according to claim 92, wherein the compound is a compound according to any one of claims 1, 16, 34 or 61.

94. A method according to claim 92, wherein the patient is a human.

95. A method according to claim 92, wherein the compound is present in the blood o the patient at a concentration of 100 nanomolar or less.

96. A method for treating a condition responsive to capsaicin receptor modulation in a patient, comprising administering to the patient a capsaicin receptor modulatory amount of at least one compound or salt ofthe formula:

wherein: V, X, W,Y and Z are each independently N or CRi, with the proviso that at least one of V and X is N; U is N or CR₂, with the proviso that if V and X are N, then U is CR₂;

Ri is independently selected at each occurrence from hydrogen, halogen, hydroxy, cyano, amino, d-C₈alkyl, halod-C₈alkyl, Ci-C₈alkoxy, halod-C₈alkoxy and mono- and di-(Cι- C_.alkyDamino;

R₂ is: (i) hydrogen, halogen, cyano or -COOH;

(ii) Ci-C₈alkanoyl, C₂-C₈alkanone, or d-C₈carbamate, each of which is unsubstituted or substituted with from 1 to 9 substituents independently selected (iii) a group ofthe formula -R_c-M-A-R_y, wherein: R_e is C₀-C₃alkyl;

M is a bond, N(R_Z), O, S, SO₂, -N(R_Z)-, (C=O)_pN(R_z), N(R_z)(C=O)_p,

SO₂N(R_z), or N(R_z)SO₂, wherein p is 0 or 1; A is a bond or Ci-C₈alkyl, optionally substituted with from 1 to 3 substituents independently selected from R_b; and

R_y and R_z are:

(a) independently hydrogen, Cι-C₈alkyl, C -C₈alkenyl, C₂-C₈ alkynyl, C₆- Ci₀aryld-C₈alkyl, C₂-C₈alkyl ether, d-C_.alkoxy, a 4- to 10- membered carbocycle or heterocycle, or joined to R_e to form a 4- to 10-membered carbocycle or heterocycle, wherein each R_y and R_z is independently unsubstituted or substituted with from 1 to 9 substituents independently selected from R_b; or

(b) joined to form a 4- to 10-membered heterocycle that is unsubstituted or substituted with from 1 to 9 substituents independently selected from R_b;

Ari and Ar₂ are independently selected from 5- to 10-membered aromatic carbocycles and heterocycles, each of which is unsubstituted or substituted with from 1 to 3 substituents independently selected from groups ofthe fonnula LR_a; L is independently selected at each occunence from a bond, -O-, -C(=O)-, -OC(=O)-, -C(=O)O-, -O-C(=O)O-, -S(O)_m-, -NR_X-, -C(=O)NHR_x-, -NHR_xC(=O)-, -NR_xS(O)_m-,

-S(O)_mNR_x- and -N[S(O)_mR_x]S(O)m-; wherein m is independently selected at each occunence from 0, 1 and 2; and R_x is independently selected at each occunence from hydrogen and Cι-C₈alkyl; R_a is independently selected at each occunence firom: (i) hydrogen, halogen, cyano and nitro; and

(ii) Cι-C₈alkyl, C₂-C₈alkenyl, C₂-C₈alkynyl, C_-C₈alkyl ether, 3- to 10-membered heterocycles, mono- and di-(Cι-C _.alkyl) amino and (3- to 10-membered heterocycle)Cι- C₆alkyl, each of which is unsubstituted or substituted with from 1 to 9 substituents independently selected from R_b; and Rb is independently chosen at each occunence from hydroxy, halogen, amino, aminocarbonyl, amido, cyano, nitro, Cι-C₈alkyl, Cι-C₈alkoxy, Cι-C₈alkylthio, Cι-C₈alkyl ether, hydroxyCi-C₈alkyl, haloCι-C₈alkyl, phenyl, phenyl(Cι-C₈alkyl), mono-and di-(Cι- C₆alkyl)amino, (SO₂)Cι-C₈alkyl, 5- to 7-membered heterocycle and (5- to 7-membered heterocycle)(Cι-C₈alkyl); and thereby alleviating the condition in the patient.

97. A method according to claim 96, wherein the compound is a compound according to any one of claims 1, 16, 34 or 61.

98. A method according to claim 96, wherein the patient is suffering from (i) exposure to capsaicin, (ii) bum or irritation due to exposure to heat, (iii) burns or irritation due to exposure to light, (iv) burn, bronchoconstriction or irritation due to exposure to tear gas, air pollutants or pepper spray, or (v) bum or initation due to exposure to acid.

99. A method according to claim 96, wherein the compound is present in the blood ofthe animal at a concentration of 100 nanomolar or less.

100. A method according to claim 96, wherein the condition is treating asthma or chronic obstructive pulmonary disease.

101. A method for treating pain in a patient, comprising administering to a patient suffering from pain a capsaicin receptor modulatory amount of at least one compound or salt ofthe formula:

wherein:

V, X, W,Y and Z are each independently N or CRi, with the proviso that at least one of V and X is N;

U is N or CR₂, with the proviso that if V and X are N, then U is CR₂;

Ri is independently selected at each occunence firom hydrogen, halogen, hydroxy, cyano, amino, Cι-C₈alkyl, haloCι-C₈alkyl, Cι-C₈alkoxy, haloCi-C_.alkoxy and mono- and di-(Cι- C₈alkyl)amino; R is: (i) hydrogen, halogen, cyano or -COOH; (ii) Cι-C₈alkanoyl, C₂-C₈alkanone, or Cι-C₈carbamate, each of which is unsubstituted or substituted with from 1 to 9 substituents independently selected from R ; or (iii) a group ofthe formula -R_c-M-A-R_y, wherein: R_e is Co-C₃alkyl;

M is a bond, N(R_Z), O, S, SO₂, -N(R_Z)-, (C=O)_pN(R_z), N(R_z)(C=O)_p,

S0₂N(R_z), orN(R_z)SO₂, wherein p is 0 or 1; A is a bond or Cι-C₈alkyl, optionally substituted with from 1 to 3 substituents independently selected from R_b; and R_y and R_z are:

(a) independently hydrogen, Cι-C₈alkyl, C₂-C₈alkenyl, C₂-C₈ alkynyl, C₆- Cι₀arylCι-C₈alkyl, C₂-C₈alkyl ether, Cι-C₈alkoxy, a 4- to 10- membered carbocycle or heterocycle, or joined to R_e to form a 4- to 10-membered carbocycle or heterocycle, wherein each R_y and R_z is independently unsubstituted or substituted with from 1 to 9 substituents independently selected from R_b; or

(b) joined to form a 4- to 10-membered heterocycle that is unsubstituted or substituted with from 1 to 9 substituents independently selected from R_b; Ari and Ar₂ are independently selected from 5- to 10-membered aromatic carbocycles and heterocycles, each of which is unsubstituted or substituted with from 1 to 3 substituents independently selected from groups ofthe formula LR_a; L is independently selected at each occurrence from a bond, -O-, -C(=O)-, -OC(=O)-,

-C(=O)O-, -O-C(=O)O-, -S(O)_m-, -NR_X-, -C(=O)NHR_x-, -NHR_xC(=O)-, -NR_xS(O)_m-, -S(O)_mNR_x- and -N[S(O)_mR_x]S(O)_m-; wherein m is independently selected at each occunence from 0, 1 and 2; and R_x is independently selected at each occunence from hydrogen and Cι-C₈alkyl; R_a is independently selected at each occunence from:

(i) hydrogen, halogen, cyano and nitro; and (ii) Cι-C₈alkyl, C₂-C₈alkenyl, C₂-C₈alkynyl, d-C_.alkyl ether, 3- to 10-membered heterocycles, mono- and di-(C_-C₈alkyl)amino and (3- to 10-membered heterocycle)d-

C₆alkyl, each of which is unsubstituted or substituted with from 1 to 9 substituents independently selected from R_b; and R_b is independently chosen at each occunence from hydroxy, halogen, amino, aminocarbonyl, amido, cyano, nitro, d-C₈alkyl, d-C₈alkoxy, d-C₈alkylthio, Ci-C₈alkyl ether, hydroxyCι-C₈alkyl, haloCι-C₈alkyl, phenyl, phenyl(d-C₈alkyl), mono-and di-(Cι- C₆alkyl) amino, (S0₂)Ci-C₈alkyl, 5- to 7-membered heterocycle and (5- to 7-membered heterocycle)(Ci-C₈alkyl); and thereby alleviating pain in the patient.

102. A method according to claim 101, wherein the compound is a compound according to any one of claims 1, 16, 34 or 61.

103. A method according to claim 101, wherein the compound is present in the blood ofthe animal at a concentration of 100 nanomolar or less.

104. A method according to claim 101, wherein the patient is suffering from neuropathic pain.

105. A method according to claim 101, wherein the pain is associated with a condition selected from: postmastectomy pain syndrome, stump pain, phantom limb pain, oral neuropathic pain, toothache, postheφetic neuralgia, diabetic neuropathy, reflex sympathetic dystrophy, trigeminal neuralgia, osteoarthritis, rheumatoid arthritis, fibromyalgia, Guillain-Bane syndrome, meralgia paresthetica, buming-mouth syndrome, bilateral peripheral neuropathy, causalgia, neuritis, neuronitis, neuralgia, AEDS-related neuropathy, MS-related neuropathy, spinal cord injury-related pain, surgery-related pain, musculoskeletal pain, back pain, headache, migraine, angina, labor, hemonhoids, dyspepsia, Charcot's pains, intestinal gas, menstraation, cancer, venom exposure, irritable bowel syndrome, inflammatory bowel disease, and/or trauma.

106. A method according to claim 101, wherein the patient is a human.

107. A method for treating itch in a patient, comprising administering to a patient a capsaicin receptor modulatory amount of a compound or salt ofthe formula: wherein:

V, X, W,Y and Z are each independently N or CRi, with the proviso that at least one of V and X is N; U is N or CR₂, with the proviso that if V and X are N, then U is CR₂;

Ri is independently selected at each occunence from hydrogen, halogen, hydroxy, cyano, amino, Ci-C₈alkyl, haloCi-C₈alkyl, d-C_.alkoxy, haloCι-C₈alkoxy and mono- and di-(d- C₈alkyl)amino; R₂ is: (i) hydrogen, halogen, cyano or -COOH; (ii) Cι-C₈alkanoyl, C₂-C₈alkanone, or Cι-C₈carbamate, each of which is unsubstituted or substituted with from 1 to 9 substituents independently selected (iii) a group ofthe formula ~R_-M-A-Ry, wherein: R_e is Co-C_alkyl; M is a bond, N(R₂), O, S, SO₂, -N(R_Z)-, (C=O)_pN(R_z), N(R_z)(C=O)_p,

SO₂N(R_z), or N(R₂)SO₂, wherein p is 0 or 1; A is a bond or Cι-C₈alkyl, optionally substituted with from 1 to 3 substituents independently selected from R ; and R_y and R_z are: (a) independently hydrogen, Cι-C₈alkyl, C₂-C₈alkenyl, C₂-C₈ alkynyl, C₆-

CioarylCi-C_.alkyl, C₂-C₈alkyl ether, d-C₈alkoxy, a 4- to 10- membered carbocycle or heterocycle, or joined to R_e to form a 4- to 10-membered carbocycle or heterocycle, wherein each R_y and R_z is independently unsubstituted or substituted with from 1 to 9 substituents independently selected from R_b; or

(b) joined to form a 4- to 10-membered heterocycle that is unsubstituted or substituted with from 1 to 9 substituents independently selected firom R_b; Aii and Ar₂ are independently selected from 5- to 10-membered aromatic carbocycles and heterocycles, each of which is unsubstituted or substituted with from 1 to 3 substituents independently selected from groups ofthe formula LR^; L is independently selected at each occunence from a bond, -O-, -C(=O)-, -OC(=O)-, -C(=O)O-, -0-C(=0)0-, -S(O)_m-, -NR_X-, -C(-O)NHR_x-, -NHR_xC(=O)-, -NR_xS(0)_m-,

-S(O)_mNR_x- and -N[S(0)_mR_x]S(O)_m-; wherein m is independently selected at each occurrence from 0, 1 and 2; and R_x is independently selected at each occurrence from hydrogen and d-C₈alkyl; R_a is independently selected at each occunence from: (i) hydrogen, halogen, cyano and nitro; and

(ii) Ci-C₈alkyl, C₂-C₈alkenyl, C₂-C₈alkynyl, C -C₈alkyl ether, 3- to 10-membered heterocycles, mono- and di-(Cι-C_alkyl)amino and (3- to 10-membered heterocycle)Cι- C₆alkyl, each of which is unsubstituted or substituted with from 1 to 9 substituents independently selected from R_b; and R_b is independently chosen at each occunence from hydroxy, halogen, amino, aminocarbonyl, amido, cyano, nitro, Cι-C₈alkyl, Cι-C₈alkoxy, Cι-C₈alkylthio, Cι-C₈alkyl ether, hydroxyCι-C₈alkyl, haloCι-C₈alkyl, phenyl, phenyl(Cι-C₈alkyl), mono-and di-(Cι- C₆alkyl)amino, (SO )Cι-C₈alkyl, 5- to 7-membered heterocycle and (5- to 7-membered heterocycle)(Cι-C₈alkyl); and thereby alleviating itch in the patient.

108. A method according to claim 107, wherein the compound is a compound according to any one of claims 1, 16, 34 or 61.

109. A method for treating cough or hiccup in a patient, comprising administering to a patient a capsaicin receptor modulatory amount of a compound or salt ofthe formula:

HN"^Ar2

wherein:

V, X, W,Y and Z are each independently N or CRi, with the proviso that at least one of V and X is N; U is N or CR , with the proviso that if V and X are N, then U is CR₂;

Ri is independently selected at each occurrence from hydrogen, halogen, hydroxy, cyano, amino, Cι-C₈alkyl, haloCι-C₈alkyl, Cι-C₈alkoxy, haloCι-C₈alkoxy and mono- and di-(Cι- C₈alkyl)amino; R₂ is: (i) hydrogen, halogen, cyano or -COOH;

(ii) Cι-C₈alkanoyl, C₂-C₈alkanone, or Cι-C₈carbamate, each of which is unsubstituted or substituted with from 1 to 9 substituents independently selected (iii) a group ofthe formula -R_-M-A-R_y, wherein: R_e is C₀-C₃alkyl;

M is a bond, N(R_), O, S, SO₂, -N(R_Z)-, (C=O)_pN(R_z), N(R_z)(C=O)_p,

SO₂N(R_z), or N(R_z)SO₂, wherein p is 0 or 1; A is a bond or Ci-C _.alkyl, optionally substituted with from 1 to 3 substituents independently selected from R ; and R_y and R_z are:

(a) independently hydrogen, Cι-C₈alkyl, C₂-C₈alkenyl, C₂-C₈ alkynyl, C₆- Ci₀arylCi-C₈alkyl, C₂-C₈alkyl ether, Cι-C₈alkoxy, a 4- to 10- membered carbocycle or heterocycle, or joined to R_e to form a 4- to 10-membered carbocycle or heterocycle, wherein each R_y and R_z is independently unsubstituted or substituted with from 1 to 9 substituents independently selected from R_b; or

(b) joined to form a 4- to 10-membered heterocycle that is unsubstituted or substituted with from 1 to 9 substituents independently selected from

R_b; Ari and Ar₂ are independently selected from 5- to 10-membered aromatic carbocycles and heterocycles, each of which is unsubstituted or substituted with from 1 to 3 substituents independently selected from groups ofthe formula LR_a; L is independently selected at each occunence from a bond, -O-, -C(=O)-, -OC(=O)-, -C(=O)O-, -O-C(=O)O-, -S(O)_m-, -NR_X-, -C(=O)NHR_x-, -NHR_xC(=O)-, -NR_xS(O)_m-, -S(0)_mNR_x- and -N[S(O)_mR_x]S(O)_m-; wherein m is independently selected at each occurrence from 0, 1 and 2; and R_x is independently selected at each occunence from hydrogen and Cι-C₈alkyl; R_a is independently selected at each occunence from: (i) hydrogen, halogen, cyano and nitro; and

(ii) Cι-C₈alkyl, C₂-C₈alkenyl, C_-C₈alkynyl, C₂-C₈alkyl ether, 3- to 10-membered heterocycles, mono- and di-(Cι-C_alkyl)amino and (3- to 10-membered heterocycle)Cι- C_.alkyl, each of which is unsubstituted or substituted with from 1 to 9 substituents independently selected from R_b; and

R_b is independently chosen at each occunence from hydroxy, halogen, amino, aminocarbonyl, amido, cyano, nitro, Cι-C₈alkyl, Cι-C₈alkoxy, Cι-C₈alkylthio, Cι-C₈alkyl ether, hycfroxyd-C_. alkyl, haloCι-C₈alkyl, phenyl, phenyl(Cι-C₈alkyl), mono-and di-(Cι-

C₆alkyl)amino, (SO )Cι-C₈alkyl, 5- to 7-membered heterocycle and (5- to 7-membered heterocycle)(Cι-C₈alkyl); and thereby alleviating cough or hiccup in the patient.

110. A method according to claim 109, wherein the compound is a compound according to any one of claims 1, 16, 34 or 61.

111. A method promoting weight loss in an obese patient, comprising administering to a patient a capsaicin receptor modulatory amount of a compound or salt of the formula:

wherein:

V, X, W,Y and Z are each independently N or CRi, with the proviso that at least one of V and

X is N; U is N or CR₂, with the proviso that if V and X are N, then U is CR₂;

Ri is independently selected at each occunence from hydrogen, halogen, hydroxy, cyano, amino, Cι-C₈alkyl, haloCι-C₈alkyl, Cι-C₈alkoxy, haloCi-C_.alkoxy and mono- and di-(Cι-

C₈alkyl)amino; R₂ is: (i) hydrogen, halogen, cyano or -COOH;

(ii) Cι-C₈alkanoyl, d-Csalkanone, or Cι-C₈carbamate, each of which is unsubstituted or substituted with from 1 to 9 substituents independently selected (iii) a group ofthe formula -R_c-M-A-R_y, wherein: R_e is Co-C₃alkyl;

M is a bond, N(R_Z), O, S, S0₂, -N(R₂>, (C=O)_pN(R_z), N(R_z)(C=0)_p, SO₂N(R_z), or N(R_z)SO₂, wherein p is 0 or 1; A is a bond or Cι-C_alkyl, optionally substituted with from 1 to 3 substituents independently selected firom R_b; and R_y and R_z are:

(a) independently hydrogen, Cι-C₈alkyl, C₂-C₈alkenyl, C₂-C₈ alkynyl, C - CioarylCi-C₈alkyl, C₂-C₈alkyl ether, Cι-C₈alkoxy, a 4- to 10- membered carbocycle or heterocycle, or joined to R_e to form a 4- to

10-membered carbocycle or heterocycle, wherein each R_y and R_z is independently unsubstituted or substituted with from 1 to 9 substituents independently selected from R_b; or

(b) joined to form a 4- to 10-membered heterocycle that is unsubstituted or substituted with from 1 to 9 substituents independently selected from

R_b;

Ari and Ar₂ are independently selected from 5- to 10-membered aromatic carbocycles and heterocycles, each of which is unsubstituted or substituted with from 1 to 3 substituents independently selected from groups ofthe formula LR_a; L is independently selected at each occurrence from a bond, -O-, -C(=O)-, -OC(=O)-, -C(=O)O-, -O-C(=O)O-, -S(O)_m-, -NR_X-, -C(=O)NHR_x-, -NHR_xC(=0)-, -NR_xS(O)_m-, -S(O)_mNR_x- and -N[S(O)_mR_x]S(O)_m-; wherein m is independently selected at each occunence from 0, 1 and 2; and R_x is independently selected at each occunence from hydrogen and Cι-C₈alkyl; R_a is independently selected at each occunence from: (i) hydrogen, halogen, cyano and nitro; and

(ii) Cι-C₈alkyl, C₂-C₈alkenyl, C₂-C₈alkynyl, C₂-C₈alkyl ether, 3- to 10-membered heterocycles, mono- and di-(Cι-C₈alkyl)amino and (3- to 10-membered heterocycle)d- C₆alkyl, each of which is unsubstituted or substituted with from 1 to 9 substituents independently selected from R_b; and

Rb is independently chosen at each occunence from hydroxy, halogen, amino, aminocarbonyl, amido, cyano, nitro, Cι-C₈alkyl, Cι-C₈alkoxy, Cι-C₈alkylthio, Cι-C₈alkyl ether, hydroxyCι-C₈alkyl, haloCι-C₈alkyl, phenyl, phenyl(Cι-C₈alkyl), mono-and di-(d- C₆alkyl)amino, (S0₂)Ci-C₈alkyl, 5- to 7-membered heterocycle and (5- to 7-membered heterocycle)(Cι-C₈alkyl); and thereby promoting weight loss in the patient.

112. A method according to claim 111, wherein the compound is a compound according to any one of claims 1, 16, 34 or 61.

113. A compound or salt according to any one of claims 1, 16, 34 or 61, wherem the compound or salt is radiolabeled.

114. A method for determining the presence or absence of capsaicin receptor in a sample, comprising the steps of:

(a) contacting a sample with a compound or salt according to any one of claims 1, 16, 34 or 61 under conditions that permit binding ofthe compound to capsaicin receptor; and (b) detecting a level of the compound bound to capsaicin receptor, and therefrom determining the presence or absence of capsaicin receptor in the sample.

115. A method according to claim 114, wherein the compound is a radiolabeled compound according to claim 113, and wherein the step of detection comprises the steps of: (i) separating unbound compound from bound compound; and

(ii) detecting the presence or absence of bound compound in the sample.

116. A packaged pharmaceutical preparation, comprising:

(a) a pharmaceutical composition according to claim 81 in a container; and (b) instractions for using the composition to treat pain.

117. A packaged pharmaceutical preparation, comprising:

(a) a pharmaceutical composition according to claim 81 in a container; and

(b) instructions for using the composition to treat cough or hiccup.

118. A packaged pharmaceutical preparation, comprising:

(a) a pharmaceutical composition according to claim 81 in a container; and

(b) instructions for using the composition to treat obesity.

119. Use of a compound according to any one of claims 1, 16, 34 or 61 for the manufacture of a medicament for treating a condition responsive to capsaicin receptor modulation in a patient.

120. Use according to claim 119, wherein the condition is selected from bum, pain, itch, cough, hiccup or bronchoconstriction.