WO2007108968A2 - Ophthalmic compositions for treating ocular hypertension - Google Patents
Ophthalmic compositions for treating ocular hypertension Download PDFInfo
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- WO2007108968A2 WO2007108968A2 PCT/US2007/006109 US2007006109W WO2007108968A2 WO 2007108968 A2 WO2007108968 A2 WO 2007108968A2 US 2007006109 W US2007006109 W US 2007006109W WO 2007108968 A2 WO2007108968 A2 WO 2007108968A2
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- methoxy
- oxoquinolin
- acetamide
- butyl
- cyclopentyl
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- 0 COc1ccc(C=C(C(*2CC(O)=O)=O)c3ccccc3)c2c1 Chemical compound COc1ccc(C=C(C(*2CC(O)=O)=O)c3ccccc3)c2c1 0.000 description 7
- MJOCXMIFNYBINK-UHFFFAOYSA-N CC(C)(C)CCNCCO Chemical compound CC(C)(C)CCNCCO MJOCXMIFNYBINK-UHFFFAOYSA-N 0.000 description 1
- WEGKKDODHXLCFG-UHFFFAOYSA-N CC(C)CCC(C(C(C1CCCCC1)=CC1=CC2)=O)=CC1=CC2OC Chemical compound CC(C)CCC(C(C(C1CCCCC1)=CC1=CC2)=O)=CC1=CC2OC WEGKKDODHXLCFG-UHFFFAOYSA-N 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D215/00—Heterocyclic compounds containing quinoline or hydrogenated quinoline ring systems
- C07D215/02—Heterocyclic compounds containing quinoline or hydrogenated quinoline ring systems having no bond between the ring nitrogen atom and a non-ring member or having only hydrogen atoms or carbon atoms directly attached to the ring nitrogen atom
- C07D215/16—Heterocyclic compounds containing quinoline or hydrogenated quinoline ring systems having no bond between the ring nitrogen atom and a non-ring member or having only hydrogen atoms or carbon atoms directly attached to the ring nitrogen atom with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to ring carbon atoms
- C07D215/20—Oxygen atoms
- C07D215/22—Oxygen atoms attached in position 2 or 4
- C07D215/227—Oxygen atoms attached in position 2 or 4 only one oxygen atom which is attached in position 2
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P27/00—Drugs for disorders of the senses
- A61P27/02—Ophthalmic agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P27/00—Drugs for disorders of the senses
- A61P27/02—Ophthalmic agents
- A61P27/06—Antiglaucoma agents or miotics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D215/00—Heterocyclic compounds containing quinoline or hydrogenated quinoline ring systems
- C07D215/02—Heterocyclic compounds containing quinoline or hydrogenated quinoline ring systems having no bond between the ring nitrogen atom and a non-ring member or having only hydrogen atoms or carbon atoms directly attached to the ring nitrogen atom
- C07D215/16—Heterocyclic compounds containing quinoline or hydrogenated quinoline ring systems having no bond between the ring nitrogen atom and a non-ring member or having only hydrogen atoms or carbon atoms directly attached to the ring nitrogen atom with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to ring carbon atoms
- C07D215/20—Oxygen atoms
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D241/00—Heterocyclic compounds containing 1,4-diazine or hydrogenated 1,4-diazine rings
- C07D241/36—Heterocyclic compounds containing 1,4-diazine or hydrogenated 1,4-diazine rings condensed with carbocyclic rings or ring systems
- C07D241/38—Heterocyclic compounds containing 1,4-diazine or hydrogenated 1,4-diazine rings condensed with carbocyclic rings or ring systems with only hydrogen or carbon atoms directly attached to the ring nitrogen atoms
- C07D241/40—Benzopyrazines
- C07D241/44—Benzopyrazines with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to carbon atoms of the hetero ring
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D401/00—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom
- C07D401/02—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom containing two hetero rings
- C07D401/06—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom containing two hetero rings linked by a carbon chain containing only aliphatic carbon atoms
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D419/00—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen, oxygen, and sulfur atoms as the only ring hetero atoms
- C07D419/02—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen, oxygen, and sulfur atoms as the only ring hetero atoms containing two hetero rings
- C07D419/12—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen, oxygen, and sulfur atoms as the only ring hetero atoms containing two hetero rings linked by a chain containing hetero atoms as chain links
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D471/00—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, at least one ring being a six-membered ring with one nitrogen atom, not provided for by groups C07D451/00 - C07D463/00
- C07D471/02—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, at least one ring being a six-membered ring with one nitrogen atom, not provided for by groups C07D451/00 - C07D463/00 in which the condensed system contains two hetero rings
- C07D471/04—Ortho-condensed systems
Definitions
- Glaucoma is a degenerative disease of the eye wherein the intraocular pressure is too high to permit normal eye function. As a result, damage may occur to the optic nerve head and result in irreversible loss of visual function. If untreated, glaucoma may eventually lead to blindness. Ocular hypertension, i.e., the condition of elevated intraocular pressure without optic nerve head damage or characteristic glaucomatous visual field defects, is now believed by the majority of ophthalmologists to represent merely the earliest phase in the onset of glaucoma.
- This invention relates to the use of potent quinoli-2(lH)-one derivatives and their aza analogues as potassium channel blockers or a formulation thereof in the treatment of glaucoma and other conditions which are related to elevated intraocular pressure in the eye of a patient.
- This invention also relates to the use of such compounds to provide a neuroprotective effect to the eye of mammalian species, particularly humans. More particularly this invention relates to the treatment of glaucoma and/or ocular hypertension (elevated intraocular pressure) using novel quinoli-2(lH)-one derivatives and their aza analogues having the structural formula I:
- Z 5 Z] 3 Z 2 , and Z3 independently represent CH or N;
- R and RY independently represent hydrogen, or Ci_6 alkyl
- Rl represents hydrogen or C ⁇ - ⁇ alkyl, CF3, (CH2) n C3-l 0 cycloalkyl, (CH 2 ) n C6_io aryl, -(CH 2 ) n C5-i0 heteroaryl, C ⁇ . ⁇ alkoxy, OH, COR C , said alkyl, cycloalkyl, aryl, heteroaryl, and alkoxy optionally substituted with 1-3 groups selected from Rb;
- X represents -(CHR7) p -, -(CHR7) p C(O)-;
- Q represents N, CRy * or O, wherein R2 is absent when Q is O;
- R2 represents hydrogen, Ci_jo alkyl, C2-10 hydroxylalkyl, C].6 alkylSR, -(CH2)nO(CH2) m OR, (CH 2 )m0R, -(CH2)n(CHR 7 )s(CH2)mCl-6 alkoxy, -(CH2)n(CHR7)(CH 2 ) m C3-8 cycloalkyl, - (CH2)n(CHR7) s (CH2) m C3-10 heterocyclyl, -(CH2) n C5-io heteroaryl, -N(R)2, -COOR, or - (CH2)n(CHR7)s(CH2)mC6-10 3 T ⁇ sa 'd alkyl, cycloalkyl, heterocyclyl, aryl or heteroaryl optionally substituted with 1-3 groups selected from Ra;
- R3 represents hydrogen, Ci-io alkyl, C2-6 alkenyl, -(CH2) n (CHR7) s (CH2)mC3-8 cycloalkyl, - (CH2)n(CHR7)s(CH2) m C3-10 heterocyclyl, -(CH 2 )n(CHR7)(CH2) m C5-10 heteroaryl, - (CH2)n(CHR7)s(CH 2 ) m COOR, -(CH2)n(CHR 7 ) s (CH2)rnC6-l0 aryl, -(CH2) n (CHR7)s(CH 2 )mNHR8 3 - (CH2)n(CHR 7 ) s (CH2) m N(R)2, -(CH2)n(CHR7)s(CH 2 )mN(R8)2, -(CH2)n(CHR7) s (CH 2 )mNHCOOR, - (CH2)n(CHR7)s(CH2) m N(R
- R2 and R3 taken together with the intervening CRy form a 4-10 membered carbocyclic or heterocyclic aromatic ring or fused ring optionally interrupted by 1-2 atoms of O, S 5 C(O) or NR, and optionally having 1-5 double bonds, and optionally substituted by 1-3 groups selected from Ra;
- R4 represents hydrogen, Cj -6 alkoxy, halogen, cyano, OH, Ci_6 alkyl, COOR, SO3H, Ci_ 6 alkylcarbonyl, S(O)qRy, -O(CH2) n N(R)2, -O(CH2) n CO 2 R, -OPO(OH)2, CF 3 , -N(R)2, nitro, or Ci-6 alkylamino;
- R7 represents hydrogen, C ⁇ -6 alkyl, -(CH2) n COOR or -(CH2) n N(R)2,
- R8 represents -(CH2) n C3_8 cycloalkyl, -(CH2)n 3-10 heterocyclyl, Ci-6 alkoxy or -(CH2)nC5-iO heteroaryl, -(CH2)nC6-10 aryl said heterocyclyl, cycloalkyl, aryl orheteroaryl optionally substituted with 1-3 groups selected from R a ;
- Ra represents F, Cl, Br, I, CF 3 , N(R) 2 , NO 2 , CN, -(CKk) n CORs, -(CH ⁇ nCONHRg, -
- K independently represents CH, CH 2 or NH
- R w represents H, C]_ 6 alkyl, -C(O)C]_ 6 alkyl, -C(O)OCi_ 6 alkyl, -SO2N(R)2, -SO2C1-6 alkyl, -SO2C 6 _ 10 aryl, NO 2 , CN or -C(O)N(R) 2 ;
- Rb represents Ci -6 alkyl, -COOR, -SO3R, CN, (CH2) n OR, C(O)O(CH2) n C(O)R, -OPO(OH)2, - (CH2) n C6-10 aryl, or -(CH2) n C5-i0 heteroaryl;
- Rc represents hydrogen, C 1-6 alkyl, or -(CH2)nC6-10 ar yl
- the present invention is directed to novel potassium channel blockers of Formula I. It also relates to a method for decreasing elevated intraocular pressure or treating glaucoma by administration, preferably topical or intra-camaral administration, of a composition containing a potassium channel blocker of Formula I described hereinabove and a pharmaceutically acceptable carrier.
- Still another embodiment of this invention is realized when Q is N and all other variables are as originally described.
- Still another embodiment of this invention is realized when Q is CH or CCH 3 and all other variables are as originally described.
- R w is selected from H, Ci _6 alkyl, -C(O)Ci -6 alkyl and - C(O)N(R) 2 .
- a sub-embodiment of this invention is realized when QR2R3 is a dialkylamine or hydroxylarnine and all other variables are as originally described..
- Still another embodiment of this invention is realized when Ri is C] -6 alkyl, Z is N, Zi, Z2, and Z3 are each CH, and QR2R3 is a dialkylamine or hydroxyldialkylamine and all other variables are as originally described.
- R7 is hydrogen or Ci-g alkyl, and all other variables are as originally described.
- R a is selected from F, Cl 5 Br, I, CF 3 , N(R) 2 , NO 2 , CN, -CONHR8, -CON(RS)2, -O(CH2) n COOR, -NH(CEk) n OR, -COOR 1 - OCF3, -NHCOR, -SO2R, -SO2NR2, -SR 5 (C 1 -C 6 alkyl)O-, -(CH2) n O(CH2) ra OR, -(CH2) n Ci-6 alkoxy, (aryl)O-, -OH, (C 1 -C 6 alkyl)S(O) m -, H 2 N-C(NH)-, (C 1 -C 6 alkyl)C(O)-, (C 1 -C 6 alkyl)OC(O)NH
- Still another embodiment of this invention is realized when R2 and R3 are taken together with the intervening N atom form a 4-10 membered heterocyclic carbon ring optionally interrupted by 1- 2 atoms of O, S, C(O) or NR, and optionally having 1-4 double bonds, and optionally substituted by 1-3 groups selected from R a .
- heterocyclic groups are:
- Still another embodiment of this invention is realized when Q equals CRY, and R2 and R3 taken together with the intervening CRy form a 4-10 membered carbocyclic or heterocyclic aromatic ring or fused ring optionally interrupted by 1-2 atoms of O, S, C(O) or NR, and optionally having 1-5 double bonds, and optionally substituted by 1-3 groups selected from R a .
- groups are phenyl, pyridinyl, adamantyl, [l.l .l]bicyclopentyl, and the like.
- Rl represents hydrogen or Ci_6 alkyl, (CH2)nC3-lO cycloalkyl, (CH2)nC6-10 aryl, -(CH2) n C5_io heteroaryl, C] _ ⁇ alkoxy, said alkyl, cycloalkyl, aryl and alkoxy optionally substituted with 1-3 groups selected from Rb;
- R2 represents hydrogen, Ci_io alkyl, C2-10 hydroxylalkyl, (CH2) m OR, -(CH2)n(CHR7)s(CH2) m Ci-6 alkoxy, -(CH2)n(CHR7) s (CH2) m C3-8 cycloalkyl, -(CH2)n(CHR 7 ) s (CH2) m C3-10 heterocyclyl, - (CH2)nC5-io heteroaryl, or -(CH2)n(CHR7) s (CH2)mC6-10 ⁇ yU said alkyl, cycloalkyl, heterocyclyl, aryl or heteroaryl optionally substituted with 1-3 groups selected from R a ;
- R3 represents hydrogen, C] .10 alkyl, -(CH2)n(CHR7) s (CH2)mC3-8 cycloalkyl, - (CH2)n(CHR7) s (CH2)mC3-10 heterocyclyl, -(CH2)n(CHR7) s (CH2)rnC5-10 heteroaryl, or - (CH2)n(CHR7) s (CH2)mC6-10 3 W ⁇ sa *d alkyl, cycloalkyl, alkoxy, heterocyclyl, aryl or heteroaryl optionally substituted with 1-3 groups of R a ; and all other variables are as described herein.
- a sub-embodiment of the compounds of formula EL is realized when Ri is C ⁇ -6 alkyl, optionally substituted with 1 to 3 groups of Rb.
- Examples of C ⁇ . ⁇ alkyls are t-butyl, ethyl, isopropyl, methyl and the like.
- Another sub-embodiment of the compounds of formula II is realized when Rl is hydrogen.
- Still another sub-embodiment of the compounds of formula II is realized when Ri is (CH2)nC6-l 0 aryU optionally substituted with 1 to 3 groups of Rb.
- Yet another sub-embodiment of the compounds of formula H is realized when Ri is (CH2)nC3-10 cycloalkyl, optionally substituted with 1 to
- R2 and R3 are independently Ci-io alkyl, -(CH2) n (CHR7) s (CH2) m C6- 10 aryl, (CH2)n(CHR 7 ) s (CH2)rnC3_io heterocyclyl, said alkyl, heterocyclyl, aryl optionally substituted with 1-3 groups selected from R a .
- R2 and R3 are independently hydrogen, Ci_io alkyl, said alkyl, optionally substituted with 1-3 groups selected from R a .
- the compounds of the present invention may have asymmetric centers, chiral axes and chiral planes, and occur as racemates, racemic mixtures, and as individual diastereomers, with all possible isomers, including optical isomers, being included in the present invention. (See E.L. Eliel and S.H. Wile ⁇ Stereochemistry of Carbon Compounds (John Wiley and Sons, New York 1994), in particular pages 1119-1190)
- any variable e.g. aryl, heterocycle, Ri, R4 etc.
- its definition on each occurrence is independent at every other occurrence.
- combinations of substituents/or variables are permissible only if such combinations result in stable compounds.
- R a When R a is -O- and attached to a carbon it is referred to as a carbonyl group and when it is attached to a nitrogen (e.g., nitrogen atom on a pyridyl group) or sulfur atom it is referred to a N-oxide and sulfoxide group, respectively.
- a nitrogen e.g., nitrogen atom on a pyridyl group
- sulfur atom it is referred to a N-oxide and sulfoxide group, respectively.
- alkyl refers to a monovalent alkane (hydrocarbon) derived radical containing from 1 to 10 carbon atoms unless otherwise defined. It may be straight, branched or cyclic. Preferred alkyl groups include methyl, ethyl, propyl, isopropyl, butyl, t-butyl, cyclopropyl cyclopentyl and cyclohexyl. When the alkyl group is said to be substituted with an alkyl group, this is used interchangeably with "branched alkyl group”.
- Cycloalkyl is a specie of alkyl containing from 3 to 15 carbon atoms, unless otherwise defined, without alternating or resonating double bonds between carbon atoms. It may contain from 1 to 4 rings, which can be fused. Examples of such cycloalkyl elements include, but are not limited to, cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl and cycloheptyl.
- Alkenyl is C2-C6 alkenyl.
- Alkoxy refers to an alkyl group of indicated number of carbon atoms attached through an oxygen bridge, with the alkyl group optionally substituted as described herein.
- Said groups are those groups of the designated length in either a straight or branched configuration and if two or more carbon atoms in length, they may include a double or a triple bond.
- Exemplary of such alkoxy groups are methoxy, ethoxy, propoxy, isopropoxy, butoxy, isobutoxy, tertiary butoxy, pentoxy, isopentoxy, hexoxy, isohexoxy allyloxy, propargyloxy, and the like.
- Halogen refers to chlorine, fluorine, iodine or bromine.
- Aryl refers to aromatic rings e.g., phenyl, substituted phenyl and the like, as well as rings which are fused, e.g., naphthyl, phenanthrenyl and the like.
- An aryl group thus contains at least one ring having at least 6 atoms, with up to five such rings being present, containing up to 22 atoms therein, with alternating (resonating) double bonds between adjacent carbon atoms or suitable heteroatoms.
- aryl groups are phenyl, naphthyl, tetrahydronaphthyl, indanyl, biphenyl, phenanthryl, anthryl or acenaphthyl and phenanthrenyl, preferably phenyl, naphthyl or phenanthrenyl.
- Aryl groups may likewise be substituted as defined.
- Preferred substituted aryls include phenyl and naphthyl.
- heterocyclyl or heterocyclic represents a stable 3- to 7-membered monocyclic or stable 8- to 11-membered bicyclic heterocyclic ring which is either saturated or unsaturated, and which consists of carbon atoms and from one to four heteroatoms selected from the group consisting of N, O 5 and S 5 and including any bicyclic group in which any of the above-defined heterocyclic rings is fused to a benzene ring.
- the heterocyclic ring may be attached at any heteroatom or carbon atom which results in the creation of a stable structure.
- a fused heterocyclic ring system may include carbocyclic rings and need include only one heterocyclic ring.
- heterocycle or heterocyclic includes heteroaryl moieties.
- heterocyclic elements include, but are not limited to, azepinyl, benz ⁇ midazolyl, benzisoxazolyl, benzofurazanyl, benz ⁇ pyranyl, benzothiopyranyl, benzofuryl, benzothiazolyl, benzothienyl, benzoxazolyl, chromanyl, cinnolinyl, dihydrobenzofuryl, dihydrobenzothienyl, dihydrobenzothiopyranyl, dihydrobenzothiopyranyl sulfone, dihydropyrrolyl, 1,3- dioxolanyl, furyl, imidazolidinyl, imidazoiinyl, imidazolyl, indolinyl, indolyl, isochxomanyl, isoindolinyl, isoquinolinyl, isothiazolidinyl, isothiazolyl, isothiazoHdinyl, mo ⁇
- heterocycle is selected from 2-azepinonyl, benzimidazolyl, 2-diazapinonyl, dihydroimidazolyl, dihydropyrrolyl, imidazolyl, 2-imidazolidinonyl, indolyl, isoquinolinyl, morpholinyl, piperidyl, piperazinyl, pyridyl, pyrrolidinyl, 2-piperidinonyl, 2-pyrimidinonyl, 2-pyrollidinonyl, quinolinyl, tetrahydrofuryl, tetrahydroisoquinolinyl, and thienyl.
- heteroatom means O, S or N, selected on an independent basis.
- heteroaryl refers to a monocyclic aromatic hydrocarbon group having 5 or 6 ring atoms, or a bicyclic aromatic group having 8 to 10 atoms, containing at least one heteroatom, O, S or N, in which a carbon or nitrogen atom is the point of attachment, and in which one or two additional carbon atoms is optionally replaced by a heteroatom selected from O or S 3 and in which from 1 to 3 additional carbon atoms are optionally replaced by nitrogen heteroatoms, said heteroaryl group being optionally substituted as described herein.
- heterocyclic elements include, but are not limited to, benzimidazolyl, benzisoxazolyl, benzofurazanyl, benzopyranyl, benzothiopyranyl, benzofuryl, benzothiazolyl, benzothienyl, benzoxazolyl, chromanyl, cinnolinyl, dihydrobenzofuryl, dihydrobenzothienyl, dihydrobenzothiopyranyl, dihydrobenzothiopyranyl sulfone, furyl, imidazolyl, indolinyl, indolyl, isochromanyl, isoindolinyl, isoquinolinyl, isothiazolyl, naphthyridinyl, oxadiazolyl, pyridyl, pyrazinyl, pyrazolyl, pyridazinyl, pyrimidinyl, pyrrolyl, quinazolin
- This invention is also concerned with compositions and methods of treating ocular hypertension or glaucoma by administering to a patient in need thereof one of the compounds of formula I in combination with one or more of a ⁇ -adrenergic blocking agent such as timolol, betaxolol, levobetaxolol, carteolol, levobunolol, a parasympathomimetic agent such as epinephrine, iopidine, brimonidine, clonidine, para-aminoclonidine, carbonic anhydrase inhibitor such as dorzolamide, acetazolamide, metazolamide or brinzolamide, an EP4 agonist (such as those disclosed in WO 02/24647, WO 02/42268, EP 1114816, WO 01/46140 and WO 01/72268), a prostaglandin such as latanoprost, travaprost, unoprostone, rescula, S 1033 (
- hypotensive lipid (the carboxylic acid group on the D-chain link of the basic prostaglandin structure is replaced with electrochemically neutral substituents) is that in which the carboxylic acid group is replaced with a Cj_6 alkoxy group such as OCH3 (PGF2 a I-OCH3), or a ammalia group (PGF2 a 1-OH).
- Preferred potassium channel blockers are calcium activated potassium channel blockers. More preferred potassium channel blockers are high conductance, calcium activated potassium (Maxi-K) channel blockers. Maxi-K channels are a family of ion channels that are prevalent in neuronal, smooth muscle and epithelial tissues and which are gated by membrane potential and intracellular Ca2+.
- the present invention is based upon the finding that maxi-K channels, if blocked, inhibit aqueous humor production by inhibiting net solute and H2O efflux and therefore lower IOP.
- maxi-K channel blockers are useful for treating other ophthamological dysfunctions such as macular edema and macular degeneration. It is known that lowering IOP promotes blood flow to the retina and optic nerve. Accordingly, the compounds of this invention are useful for treating macular edema and/or macular degeneration.
- maxi-K channel blockers which lower IOP are useful for providing a neuroprotective effect. They are also believed to be effective for increasing retinal and optic nerve head blood velocity and increasing retinal and optic nerve oxygen by lowering 1OP 3 which when coupled together benefits optic nerve health. As a result, this invention further relates to a method for increasing retinal and optic nerve head blood velocity, increasing retinal and optic nerve oxygen tension as well as providing a neuroprotective effect or a combination thereof.
- a number of marketed drugs function as potassium channel antagonists. The most important of these include the compounds Glyburide, Glipizide and Tolbutamide. These potassium channel antagonists are useful as antidiabetic agents.
- the compounds of this invention may be combined with one or more of these compounds to treat diabetes.
- Potassium channel antagonists are also utilized as Class 3 antiarrhythmic agents and to treat acute infarctions in humans.
- a number of naturally ammalian toxins are known to block potassium channels including Apamin, Iberiotoxin, Charybdotoxin, Noxiustoxin, Kaliotoxin, Dendrotoxin(s), mast cell degranuating (MCD) peptide, and ⁇ -Bungarotoxin ( ⁇ -BTX).
- the compounds of this invention may be combined with one or more of these compounds to treat arrhythmias.
- Depression is related to a decrease in neurotransmitter release.
- Current treatments of depression include blockers of neurotransmitter uptake, and inhibitors of enzymes involved in neurotransmitter degradation which act to prolong the lifetime of neurotransmitters.
- Alzheimer's disease is also characterized by a diminished neurotransmitter release.
- Three classes of drugs are being investigated for the treatment of Alzheimer's disease cholinergic potentiators such as the anticholinesterase drugs (e.g., physostigmine (eserine), and Tacrine (tetrahydroaminocridine)), nootropics that affect neuron metabolism with little effect elsewhere (e.g., Piracetam, Oxiracetam; and those drugs that affect brain vasculature such as a mixture of ergoloid mesylates amd calcium channel blocking drugs including Nimodipine.
- anticholinesterase drugs e.g., physostigmine (eserine), and Tacrine (tetrahydroaminocridine)
- nootropics that affect neuron metabolism with little effect elsewhere
- Piracetam, Oxiracetam e.g., Piracetam, Oxiracetam
- those drugs that affect brain vasculature such
- Selegiline a monoamine oxidase B inhibitor which increases brain dopamine and norepinephrine has reportedly caused mild improvement in some Alzheimer's patients.
- Aluminum chelating agents have been of interest to those who believe Alzheimer's disease is due to aluminum toxicity.
- Drugs that affect behavior, including neuroleptics, and anxiolytics have been employed.
- Anxiolytics, which are mild tranquilizers, are less effective than neuroleptics
- the present invention is related to novel compounds which are useful as potassium channel antagonists.
- the compounds within the scope of the present invention exhibit potassium channel antagonist activity and thus are useful in disorders associated with potassium channel malfunction.
- a number of cognitive disorders such as Alzheimer's Disease, memory loss or depression may benefit from enhanced release of neurotransmitters such as serotonin, dopamine or acetylcholine and the like.
- Blockage of Maxi-K channels maintains cellular depolarization and therefore enhances secretion of these vital neurotransmitters.
- the compounds of this invention may be combined with anticholinesterase drugs such as physostigmine (eserine) and Tacrine (tetrahydroaminocridine), nootropics such as Piracetam, Oxiracetam, ergoloid mesylates, selective calcium channel blockers such as Nimodipine, or monoamine oxidase B inhibitors such as Selegiline, in the treatment of Alzheimer's disease.
- anticholinesterase drugs such as physostigmine (eserine) and Tacrine (tetrahydroaminocridine)
- nootropics such as Piracetam, Oxiracetam, ergoloid mesylates
- selective calcium channel blockers such as Nimodipine
- monoamine oxidase B inhibitors such as Selegiline
- the compounds of this invention may also be combined with Apamin, Iberiotoxin, Charybdotoxin, Noxiustoxin, Kaliotoxin, Dendrotoxin(s), mast cell degranuating (MCD) peptide, ⁇ -Bungarotoxin ( ⁇ -BTX) or a combination thereof in treating arrythmias.
- the compounds of this invention may further be combined with Glyburide, Glipizide, Tolbutamide or a combination thereof to treat diabetes.
- each of the claimed compounds are potassium channel antagonists and are thus useful in the decribed neurological disorders in which it is desirable to maintain the cell in a depolarized state to achieve maximal neurotransmitter release.
- the compounds produced in the present invention are readily combined with suitable and known pharmaceutically acceptable excipients to produce compositions which may be administered to mammals, including humans, to achieve effective potassium channel blockage.
- salts of the compounds of formula I will be.pharmaceutically acceptable salts.
- Other salts may, however, be useful in the preparation of the compounds according to the invention or of their pharmaceutically acceptable salts.
- suitable “pharmaceutically acceptable salts” refers to salts prepared form pharmaceutically acceptable non-toxic bases including inorganic bases and organic bases. Salts derived from inorganic bases include aluminum, ammonium, calcium, copper, ferric, ferrous, lithium, magnesium, manganic salts, manganous, potassium, sodium, zinc and the like. Particularly preferred are the ammonium, calcium, magnesium, potassium and sodium salts.
- Salts derived from pharmaceutically acceptable organic non-toxic bases include salts of primary, secondary and tertiary amines, substituted, amines including naturally occurring substituted amines, cyclic amines and basic ion exchange resins, such as arginine, betaine caffeine, choline, NjN'-dibenzylethylenediami ⁇ e, diethylamin, 2-diethylaminoethanol, 2-dimethylaminoethanol, ethanolamine, ethylenediamine, N-ethylmorpholine, N-ethylpiperidine, glucamine, glucosamine, histidine, hydrabamine, isopropylamine, lysine, methylglucamine, morpholine, piperazine, piperidine, polyamine resins, procaine, purines, theobromine, triethylamine, trimethylamine tripropylamine, tromethamine and the like.
- basic ion exchange resins such as argin
- salts may be prepared from pharmaceutically acceptable non-toxic acids, including inorganic and organic acids.
- acids include acetic, benzenesulfonic, benzoic, camphorsulfonic, citric, ethanesulfonic, fumaric, gluconic, glutamic, hydrobromic, hydrochloric, isethion ⁇ c, lactic, maleic, malic, mandelic, methanesulfonic, mucic, nitric, pamoic, pantothenic, phosphoric, succinic, sulfuric, tartaric, p-toluenesulfonic acid and the like.
- Particularly preferred are citric, hydrobromic, hydrochloric, maleic, phosphoric, sulfuric and tartaric acids.
- composition is intended to encompass a product comprising the specified ingredients in the specific amounts, as well as any product which results, directly or indirectly, from combination of the specific ingredients in the specified amounts.
- the daily dosage will normally be determined by the prescribing physician with the dosage generally varying according to the age, weight, sex and response of the individual patient, as well as the severity of the patient's symptoms.
- maxi-K channel blockers used can be administered in a therapeutically effective amount intravaneously, subcutaneously, topically, transdermally, parenterally or any other method known to those skilled in the art.
- Ophthalmic pharmaceutical compositions are preferably adapted for topical administration to the eye in the form of solutions, suspensions, ointments, creams or as a solid insert.
- Ophthalmic formulations of this compound may contain from 0.01 ppm to 1% and especially 0.1 ppm to 1% of medicament. Higher dosages as, for example, about 10% or lower dosages can be employed provided the dose is effective in reducing intraocular pressure, treating glaucoma, increasing blood flow velocity or oxygen tension.
- For a single dose from between 1 ng to 500ug, preferably 1 ng to 500 ug, of the compound can be applied to the human eye.
- the pharmaceutical preparation which contains the compound may be conveniently admixed with a non-toxic pharmaceutical organic carrier, or with a non-toxic pharmaceutical inorganic carrier.
- a non-toxic pharmaceutical organic carrier or with a non-toxic pharmaceutical inorganic carrier.
- pharmaceutically acceptable carriers are, for example, water, mixtures of water and water-miscible solvents such as lower alkanols or aralkanols, vegetable oils, polyalkylene glycols, petroleum based jelly, ethyl cellulose, ethyl oleate, carboxymethyl-cellulose, polyvinylpyrrolidone, isopropyl myristate and other conventionally employed acceptable carriers.
- the pharmaceutical preparation may also contain non-toxic auxiliary substances such as emulsifying, preserving, wetting agents, bodying agents and the like, as for example, polyethylene glycols 200, 300, 400 and 600, carbowaxes 1,000, 1,500, 4,000, 6,000 and 10,000, antibacterial components such as quaternary ammonium compounds, phenylmercuric salts known to have cold sterilizing properties and which are non-injurious in use, thimerosal, methyl and propyl paraben, benzyl alcohol, phenyl ethanol, buffering ingredients such as sodium borate, sodium acetates, gluconate buffers, and other conventional ingredients such as sorbitan monolaurate, triethanolamine, oleate, polyoxyethylene sorbitan monopalmitylate, dioctyl sodium sulfosuccinate, monothioglycerol, thiosorbitol, ethylenediamine tetracetic acid, and the like.
- auxiliary substances such as e
- suitable ophthalmic vehicles can be used as carrier media for the present purpose including conventional phosphate buffer vehicle systems, isotonic boric acid vehicles, isotonic sodium chloride vehicles, isotonic sodium borate vehicles and the like.
- the pharmaceutical preparation may also be in the form of a microparticle formulation.
- the pharmaceutical preparation may also be in the form of a solid insert. For example, one may use a solid water soluble polymer as the carrier for the medicament.
- the polymer used to form the insert may be any water soluble non-toxic polymer, for example, cellulose derivatives such as methylcellulose, sodium carboxymethyl cellulose, (hydroxyloweralkyl cellulose), hydroxyethyl cellulose, hydroxypropyl cellulose, hydroxypropylmethyl cellulose; acrylates such as polyacrylic acid salts, ethylacrylates, polyactylamides; natural products such as gelatin, alginates, pectins, tragacanth, karaya, chondrus, agar, acacia; the starch derivatives such as starch acetate, hydroxymethyl starch ethers, hydroxypropyl starch, as well as other synthetic derivatives such as polyvinyl alcohol, polyvinyl pyrrol idone, polyvinyl methyl ether, polyethylene oxide, neutralized carbopol and xanthan gum, gellan gum, and mixtures of said polymer.
- cellulose derivatives such as methylcellulose, sodium carboxy
- Suitable subjects for the administration of the formulation of the present invention include primates, man and other animals, particularly man and domesticated animals such as cats and dogs.
- the pharmaceutical preparation may contain non-toxic auxiliary substances such as antibacterial components which are non-injurious in use, for example, thimerosal, benzalkonium chloride, methyl and propyl paraben, benzyldodecinium bromide, benzyl alcohol, or phenylethanol; buffering ingredients such as sodium chloride, sodium borate, sodium acetate, sodium citrate, or gluconate buffers; and other conventional ingredients such as sorbitan monolaurate, triethanolamine, polyoxyethylene sorbitan monopalmitylate, ethyl enediamine tetraacetic acid, and the like.
- auxiliary substances such as antibacterial components which are non-injurious in use, for example, thimerosal, benzalkonium chloride, methyl and propyl paraben, benzyldodecinium bromide, benzyl alcohol, or phenylethanol
- buffering ingredients such as sodium chloride, sodium borate, sodium acetate,
- the ophthalmic solution or suspension may be administered as often as necessary to maintain an acceptable IOP level in the eye. It is contemplated that administration to the ammalian eye will be about once or twice daily.
- novel formulations of this invention may take the form of solutions, gels, ointments, suspensions or solid inserts, formulated so that a unit dosage comprises a therapeutically effective amount of the active component or some multiple thereof in the case of a combination therapy.
- AIBN 2,2'-azobisisobutyronitrile, DCM - Dichloromethane, mCPBA — meta-Chloroperbenzoic acid,
- the core heterocycle of.l,6-naphthy ⁇ din-2(lH)-one 1 can be prepared as shown in Scheme 8.
- Substituted analogues could be prepared from a more elaborated starting material or obtained by further transformation of the un-substituted heterocycle using the method depicted in Scheme 9.
- Step D Ethyl 2-[(2-amino-4-methoxyphenyl)(hydroxy)methyl]-3,3-dimethylbutanoate
- Steps B and C (7-Methoxy-2-oxoquinolin-l(2H)-yl)acetic acid
- the title compound was prepared using the procedure described in Steps F-G of Preparative Example 1 and starting with 7-methoxyquinolin- 2(lH)-one from the Step A above.
- N-butyl-3,3-dimethylbutanamide was prepared from n-butylamine, t-butylacetyl chloride, and DIEA.
- the crude amide was reduced in refluxing benzene with 1.5 molar equiv. of LAH in 2 hours.
- the excess LAH was quenched with MeOH and 1 ⁇ KOH after cooling.
- the resulting mixture was filtered and the solid washed with ether.
- the residue from the organic layer was dissolved in ether and treated with 1 ⁇ HCl in ether to precipitate the title compound.
- Step B 4-[(3 3 3-Dimethylbutyl)amino]-2-methylbutan-2-ol
- the title compound of Step C can also be obtained as follows. A mixture of 50 mg methyl N-(6- methoxy-3- ⁇ itropyridin-2-yI)glycinate, 37 mg 3,3-dimethyl-2-oxobutanoic acid, 48 mg HOBt, and 41.7 mg DIEA in 1 mL dry DMF was treated with 79.8 mg EDC. The title compound was isolated on RP- HPLC. Similar result was obtained using PyBOP instead of EDC.
- Step D (2-ter/-Butyl-6-methoxy-3-oxo ⁇ yrido[2,3- ⁇ ]pyrazin-4(3H)-yl)acetic acid
- Step A tert-Butyl N-(6-methoxy-3-nitropyridin-2-yl)glycinate
- Step B tert-Butyl (2-fer/-butyl-6-methoxy-3-oxo ⁇ yrido[2,3-6]pyrazin-4(3H)-yl)acetate
- the title compound was prepared from 4-methoxybenzene-l,2-diamine and 3,3-dimethyl-2- oxobutanoic acid in ⁇ OAc at 48 ⁇ 49°C overnight as described in Preparative Example 50 Step C. It was separated from isomeric side-product 3-rer/-butyl-6-methoxyquinoxalin-2(lH)-one by SGC.
- Step B Methyl (3-ter ⁇ butyl-7-methoxy ⁇ 2-oxoquinoxalin-l(2H)-yl)acetate
- Step B ⁇ ert-Butyl (3-rer/'-butyl-7-methoxy-2-oxoquinoxalin-l(2i ⁇ )-yl)acetate
- Step A N-Butyl-3-hydroxy-2,2-dimethylpropanamide
- a mixture of 4.54 g 3-hydroxy-2,2- dimethylpropanoic acid, 4.21 g n-butylamine, 7.78 g HOBt 5 and 14.89 g DEEA in 100 mL DMF was treated with 18.4 g EDC overnight at room temperature.
- Solvent was removed under reduced pressure and residue was diluted with saturated NH 4 Cl solution and extracted with EtOAc. The combined extract was washed with water and saturated brine and concentrated to give the title compound.
- Step A 5-Methoxy-N-(4-methoxybenzyl)-2-nitroaniline
- the title compound was prepared from 3-fluoro- 4-nitrophenol 5 methyl iodide, and 4-methoxybenzylamine using the procedure described in Preparative Example 51 Method B Step A.
- Step B Methyl ⁇ 4-[3-(acetyIoxy)propyl]phenyl ⁇ (oxo)acetate
- a solution of 5 g 3-phenyl propylacetate and 4.1 g methyl oxalyl chloride in 20 mL DCM was added slowly to a mixture of 8.2 g anhydrous aluminum chloride in 30 mL DCM at 0 0 C.
- the reaction mixture is then allowed to warm up to room temperature overnight.
- Aqueous work-up with saturated NHjCl and EtOAc followed by SGC using 3:1 hexanes and EtOAc afforded the title compound as clear oil.
- Step C 4-Methoxy-N 2 -(4-methoxybenzyl)benzene-l,2-diamine
- Step D 3 - ⁇ 4-[6-Methoxy-4-(4-methoxybenzyl)-3 -oxo-3,4-dihydroquinoxalin-2-yI]phenyl ⁇ propyl acetate
- the title compound was prepared from 4-methoxy-iV 2 -(4-methoxybenzyl)-benzene-l,2-diamine and methyl ⁇ 4-[3-(acetyloxy)propyl] ⁇ henyl ⁇ (oxo)acetate in HOAc at 45°C in 6 hours. Similar reaction in THF was very slow. SGC purification using hexanes-EtOAc afforded pure title compound. LC-MS: 3.97 min. (m/Z 473.1).
- Step E 3-[4-(6-Methoxy-3-oxo-3,4-dihydroquinoxalin-2-yl)phenyl]propyl acetate
- Step F 3-[4-(3-Hydroxypropyl)phenyl]-7-methoxyquinoxalin-2(lH)-one
- Step G Methyl [3-[4-(3-hydroxypropyl)phenyl]-7-methoxy-2-oxoquinoxalin-l(2H)-yl]acetate
- Step B Methyl ⁇ 4-[(acetyloxy)methyl]phenyl ⁇ (oxo)acetate
- Step C 4-[6-Methoxy-4-(4-methoxybenzyl)-3-oxo-3,4-dihydroquinoxalin-2-yl]benzyl acetate
- the title compound was prepared from methyl ⁇ 4-[(acetyloxy)methyl]phenyl ⁇ (oxo)acetate and 4- methoxy-N 2 -(4-methoxybenzyl)benzene-l,2-diamine using method described in Preparative Example 57 Step D.
- the title compound was prepared from 4,4-dimethylcyclohexane-l 5 3-dione using the method of Langley et al. (J. Chem. Soc. 1962, 2972). It was obtained as a 6:5 mixture of 3,3-dimethyl-2- oxohexanedioic acid and 2,2-dimethylpentanedioic acid based on 1 H NMR and was used in the next step without purification.
- Step B 4- ⁇ 4- [6-Methoxy-4-(4-methoxy benzy l)-3-oxo-3 ,4-dihydroquinoxal in-2-y l]phenyl ⁇ -4- methylpentanoic acid
- Step C 4-[4-(6 ⁇ Methoxy-3-oxo-3,4-dihydroquinoxalin-2-yl)phenyl]-4-methylpentanoic acid
- Step D 3-[4-(4-Hydroxy- 1 , 1 -dimethylbutyl)phenyl]-7-methoxyquinoxalin-2( 1 H)-one
- Step E Methyl [3-(4-hydroxy-l,l-dimethylbutyI)-7-methoxy-2-oxoquinoxal ⁇ n-l(2H)-yl]acetate
- Step F [3-(4-Hydroxy-l,l-dimethylbutyI)-7-methoxy-2-oxoquinoxalin-l(2H)-yl]acetic acid
- Step B.3-ter/-Butyl-l,8-naphthyridin-2(lH)-one The title compound was prepared from 5.9 g methyl 2- [ ⁇ 2-[(2,2-dimethylpropanoyl)amino]pyridin-3-yl ⁇ (hydroxy)methyI]-3,3-dimethylbutanoate in 20 mL dioxane and 35 mL 4 N HCl in dioxane by heating at 170 0 C in a microwave reactor for 2 hours in three portions. The solvent was removed from the combined reaction mixture under reduced pressure and the residue was suspended in water and EtOAc after being neutralized to p ⁇ 7. The title compound was obtained by filtration.
- Step C 3-/ert-Butyl-l,8-naphthyridin-2(lH)-one 8-oxide
- the title compound was prepared by heating a solution of 2.05 g 3-ter/-butyl ⁇ l,8-naphthyridin-2(lH)-one in 20 mL 35% peracetic acid in acetic acid at 50 0 C overnight. Aqueous work-up with EtOAc afforded the title compound.
- Step D 3-ter/-Butyl-7-methoxy-l,8-naphthyridin-2(lH)-one
- the title compound was prepared from 3-/er/-butyl-l,8-naphthyridin-2(lH)-one 8-oxide using a modified method of ⁇ ayashida etal. ( ⁇ eterocycles, 31 (7), 1325, 1990) with 10 equiv. of pTsCI and 20 equiv. OfEt 3 N.
- Step E Methyl (3-fer/-butyl-7-methoxy-2-oxo-l,8- ⁇ aphthyridin-l(2i7)-yl)acetate
- the title compound was prepared from 3-tert-butyl-7-methoxy-l,8-naphthyridin-2(lH)-one and methyl bromoacetate using the method described in Preparative Example 57 Step G. It was separated from the faster-el uting isomeric methyl [(3-t ⁇ r/-butyl-7-methoxy-l,8-naphthyridin-2-yl)oxy]acetate on RP- ⁇ PLC. LC-MS: 3.54 min. (m/Z 305.1).
- the title compound was prepared from methyl (3-.”erf-butyl-7-methoxy-2-oxo-l,8-naphthyridin-l(2H)-yl)acetate using the method described in Preparative Example 57 Step ⁇ .
- Example 2 Utilizing the method described in Example 1 using (3-?er/-butyl-7-methoxy-2-oxoquinolin- l(2H)-yl)acetic acid and the amine listed in Table 4 below Examples 2 through 47 in Table 4 were prepared. In Examples 32-36, PyBOP and HOAt were used instead of EDC and HOBt.
- the title compound was prepared from 50 mg 3-ter/-butylquinolin-2(lH)-one (Preparative Example 1, Step E), 55 mg l-bromo-3,3-dimethylbutane, and 143 mg cesium carbonate in 1 mL DMF at 55°C for 12 hours. It was separated from less polar side-product 3-ter*-butyl-2-(3,3-dimethylbutoxy)-7- methoxyquinoline using RP- ⁇ PLC. The isomers were identified by comparison of NMR with isomers in Preparative Example 1, Step F.
- the title compound was prepared from 50 mg 3-/er/-butylquinolin-2(lH)-one (Preparative Example 1, Step E), 50 mg l-bromo-3,3-dimethylbutane, and 143 mg cesium carbonate in 1 mL DMF at 55°C for 12 hours. It was separated purified from less polar side-product 3-ter/-butyl-2-(3- methylbutoxy)-7-methoxyquinoline using ⁇ PLC.
- the title compound was prepared from (3-isopr ⁇ yl-2-oxoquinolin-l(2H)-yl)acetic acid from Preparative Example 2, EDC, ⁇ OBt, and DIEA using the method in Example 1, purified on ⁇ PLC, and re-crystallized from EtOAc-hexanes to give colorless crystals.
- LC-MS 4.17 min. (m/Z 437.1).
- the title compound was prepared from (3-cyclohexyl-2-oxoquinolin-l(2H)-yl)acetic acid from Preparative Example 3, EDC, ⁇ OBt, and DIEA using the method in Example 1, purified on ⁇ PLC, and re-crystallized from EtOAc-hexanes to give colorless crystals.
- Example 124 PyBOP and HO At were used instead of EDC and HOBt.
- the title compound was prepared from (2-oxo-3-phenylquinolin-l(2H)-yl)acetic acid from Preparative Example 4, EDC, HOBt, and DDBA using the method in Example 1 , purified on HPLC, and re-crystallized from EtOAc-hexanes to give colorless crystals.
- Example 287 was obtained as the side-product during the preparation of Example 286.
- Step A Di-tert-butyl 3-[(3,3-dimethylbutyl)amino]-2,2-dimethylpropyl phosphate Treat a solution of 100 mg 3-[(3,3-dimethylbutyl)amino)-2,2-dimethylpropan-l-ol and 156 mg di-Zert-butyl diethylamidophosphite in 0.6 mL anhydrous DCM with 3.5 mL of 0.45 M tetrazole in MeCN at room temperature overnight. Cool half of this mixture to -40 0 C and add a solution of 140 mg 72% mCPBA in 2.5 mL DCM. Remove the cooling bath and let the reaction mixture warm up to room temperature.
- Step C 2-[[(3-terr-Butyl-7-methoxy-2-oxoquinoxalin-l(2H)-y0 acet yl](3,3-dimethylbutyl)amino]-l,l- dimethylethyl dihydrogen phosphate
- Examples 295-301 in Table 15 were prepared using the same method described in Example 294. Some of the bromoketones used were prepared using the method of Gaudry and Marguet (Org. Syn. Coll. Vol. 6, 193).
- Step A tert-Butyl [3-(4-cyanophenyl)-7-methoxy-2-oxoquinoxalin-l(2H)-yI]acetate
- the title compound was prepared from tert-buty] iV-(5-methoxy-2-nitrophenyI)gIycinate and ethyl (4- cyanophenyl)(oxo)acetate using method described in Preparative Example 51 Method B
- Step B LC-MS: 3.75 min. (m/Z 392.0).
- Example 342 The following compounds in Table 20 were prepared using the method described in Example 1 using (3-ter/-Butyl-7-methoxy-2-oxo-l,8-naphthyridin-l(2H)-yl)acetic acid and the amine listed in Table 20.
- Step A.3-/er/-Buryl-7-chloro-l,8-naphthyridin-2(lH)-one The title compound was prepared by treating 14.5 mg 3-.eri-butyl-l,8-naphthyridin-2(lH)-one 8-oxide with 1 mL thionyl chloride at room temperature overnight. It was separated from its 7-hydroxy and 4-chloro derivatives on RP- ⁇ PLC. LC- MS: 3.21 min. (m/Z 237.1). 1 H NMR (CDCl 3 , 500 MHz) ⁇ : 7.85 (d, 8.0 Hz 3 IH), 7.64 (s, IH), 7.22 (d, 8.0 Hz 5 IH) 3 1.45 (s, 9H)
- Step A 3-/erf-Butyl-7-chloro-l-(3 3 3-dimethyl-2-oxobutyl)-l,8-naphthyr ⁇ di ⁇ -2(lH)-one
- Step A Methyl 2-[ ⁇ 4-[(2,2-dimethylpropanoyl)amino]pyridin-3-yl ⁇ (hydroxy)methyI]-3,3- dimethylbutanoate
- the title compound was prepared from N-(3-formylpyridin-4-yl)-2,2- dimethylpropanamide and methyl 3-methylbutanoate using the method of Turner (J. Org. Chem. 55, 4744, 1990).
- Step B 3-t ⁇ r/-Butyl-l,6-naphthyridin-2(l//)-one
- the title compound was prepared from methyl 2-[ ⁇ 4- [(2,2-dimethylpropanoyl)amino]pyridin-3-yl ⁇ (hydroxy )methyl]-3,3-dimethylbutanoate using the method described in Preparative Example 60 Step B by heating in a microwave reactor at 160 0 C for 2 hours.
- LC- MS 1.75 min. (m/Z 203.1).
- Step C Methyl (3-te/"/-butyl-2-oxo-t,6-naphthyridin-l(2H)-yl)acetate
- the title compound was prepared from 3-t ⁇ rr-butyl-l,6-naphthyridin-2(lH)-one and methyl bromoacetate using the method described in Preparative Example 57 Step G. It was separated from the slower-eluting isomeric methyl [(3-ter/-buryl-l,6-naphthyridin-2-yl)oxy]acetate on RP- ⁇ PLC.
- LC-MS 2.18 min. (m/Z 275.1 ).
- Step D (3-ter/'-Butyl-2-oxo-l,6-naphthyridin-l(2H)-yl)acetic acid
- the title compound was prepared from methyl (3-tert-butyl-2-oxo-l,6-naphthyridin-l(2H)-yl)acetate using the method described in Preparative Example 57 Step ⁇ .
- Step E N,iV-Dibutyl-2-(3-ferir-butyl-2-oxo- 1 ,6-naphthyridin- 1 (2H)-yI)acetamide
- the identification of inhibitors of the Maxi-K channel can be accomplished using Aurora Biosciences technology, and is based on the ability of expressed Maxi-K channels to set cellular resting potential after transient transfection of both ⁇ and ⁇ subunits of the channel in TsA-201 cells.
- cells display a hyperpolarized membrane potential, negative inside, close to E ⁇ (-80 mV) which is a consequence of the activity of the Maxi-K channel.
- Blockade of the Maxi-K channel will cause cell depolarization. Changes in membrane potential can be determined with voltage-sensitive fluorescence resonance energy transfer (FRET) dye pairs that use two components, a donor coumarin (CC 2 DMPE) and an acceptor oxanol (DiSBAC 2 (3)).
- FRET voltage-sensitive fluorescence resonance energy transfer
- Oxanol is a lipophilic anion and distributes across the membrane according to membrane potential. Under normal conditions, when the inside of the cell is negative with respect to the outside, oxanol is accumulated at the outer leaflet of the membrane and excitation of coumarin will cause FRET to occur. Conditions that lead to membrane depolarization will cause the oxanol to redistribute to the inside of the cell, and, as a consequence, to a decrease in FRET. Thus, the ratio change (donor/acceptor) increases after membrane depolarization.
- Transient transfection of the Maxi-K channel in TsA-201 cells can be carried out as previously described (Hanner et al. (1998) J. Biol. Chem. 273, 16289-16296) using FUGENE6TM as the transfection reagent. Twenty four hours after transfection, cells are collected in Ca 2+ -Mg 2+ -free Dulbecco's phosphate-buffered saline (D-PBS), subjected to centrifugation, plated onto 96-well poly-'d- lysine coated plates at a density of 60,000 cells/well, and incubated overnight.
- D-PBS Ca 2+ -Mg 2+ -free Dulbecco's phosphate-buffered saline
- the cells are then washed Ix with D-PBS, and loaded with 100 ⁇ l of 4 ⁇ M CC 2 DMPE-0.02% pluronic-127 in D-PBS. Cells are incubated at room temperature for 30 min in the dark. Afterwards, cells are washed 2x with D-PBS and loaded with 100 ⁇ l of 6 ⁇ M DiSBAC 2 (3) in (mM): 140 NaCl 5 0.1 KCl, 2 CaCl 2 , 1 MgCl 2 , 20 Hepes- NaOH, pH 7.4, 10 glucose. Test compounds are diluted into this solution, and added at the same time. Cells are incubated at room temperature for 30 min in the dark.
- the compounds of this invention were found to cause concentration-dependent inhibition of the fluorescence ratio with IC 5 o's in the range of about 5 nM to about 500 ⁇ M, more preferably from about 5 nM to about 20 nM.
- IC 5 o's concentration-dependent inhibition of the fluorescence ratio with IC 5 o's in the range of about 5 nM to about 500 ⁇ M, more preferably from about 5 nM to about 20 nM.
- maxi-K channels The activity of high-conductance calcium-activated potassium (maxi-K) channels in human non-pigmented ciliary epithelial cells was determined using electrophysiological methods. Currents through maxi-K channels were recorded in the inside-out configuration of the patch clamp technique, where the pipette solution faces the extracellular side of the channel and the bath solution faces the intracellular side. Excised patches contained one to about fifty maxi-K channels. Maxi-K channels were identified by their large single channel conductance (250-300 pS), and by sensitivity of channel gating to membrane potential and intracellular calcium concentration. Membrane currents were recorded using standard electrophysiological techniques.
- the bath (intracellular) solution was identical, except, in some cases, calcium was removed, 1 mM EGTA was added and 20 mM KCl was replaced with 20 mM KF to eliminate calcium to test for calcium sensitivity of channel gating. Drugs were applied to the intracellular side of the channel by bath perfusion.
- Human non-pigmented ciliary epithelial cells were grown in tissue culture as described (Martin-Vasallo, P., Ghosh, S., and Coca-Prados, M., 1989, J. Cell. Physiol. HL, 243-252), and plated onto glass cover slips prior to use. High resistance seals (>1 Gohm) were formed between the pipette and cell surface, and inside out patches were excised. Maxi-K channels in the patch were identified by their gating properties; channel open probability increased in response to membrane depolarization and elevated intracellular calcium. In patches used for pharmacological analysis, removing intracellular calcium eliminated voltage-gated currents. Maxi-K currents were measured after depolarizing voltage steps or ramps that caused channel opening.
- the compounds of this invention were applied to the intracellular side of the channel in appropriate concentrations (0.001 to 100 ⁇ M).
- the compounds reduced channel open probability, and this effect was reversed upon washout of compounds from the experimental chamber.
- the IC50 for block of maxi-K channels under these conditions for the compounds of this invention ranged from about 0.2 nM to about 100 ⁇ M.
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Abstract
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EP07752786A EP2004193A2 (en) | 2006-03-13 | 2007-03-09 | Ophthalmic compositions for treating ocular hypertension |
AU2007227664A AU2007227664A1 (en) | 2006-03-13 | 2007-03-09 | Ophthalmic compositions for treating ocular hypertension |
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WO2009109341A1 (en) | 2008-03-05 | 2009-09-11 | Merck Patent Gmbh | Pyridopyrazinones derivatives insulin secretion stimulators, methods for obtaining them and use thereof for the treatment of diabetes |
JP2010514677A (en) * | 2006-12-20 | 2010-05-06 | タケダ サン ディエゴ インコーポレイテッド | Glucokinase activator |
WO2010081900A1 (en) | 2009-01-19 | 2010-07-22 | Neurosearch A/S | Quinolinone derivatives useful for the treatment of cns disorders |
WO2010137547A1 (en) * | 2009-05-25 | 2010-12-02 | 国立大学法人東京工業大学 | Pharmaceutical composition containing nuclear factor involved in proliferation and differentiation of central neuronal cells |
US8367670B2 (en) | 2008-12-09 | 2013-02-05 | Gilead Sciences, Inc. | Modulators of toll-like receptors |
US8476270B2 (en) | 2009-09-14 | 2013-07-02 | Gilead Sciences, Inc. | Dihydropyrido[4,3-b]pyrazine-3-ones as modulators of toll-like receptors |
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US9365640B2 (en) | 2011-04-29 | 2016-06-14 | Celgene Corporation | Methods for the treatment of cancer and inflammatory diseases using cereblon as a predictor |
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US9675593B2 (en) | 2012-10-02 | 2017-06-13 | Intermune, Inc. | Anti-fibrotic pyridinones |
US9857359B2 (en) | 2012-06-29 | 2018-01-02 | Celgene Corporation | Methods for determining drug efficacy using cereblon-associated proteins |
US10010536B2 (en) | 2005-05-10 | 2018-07-03 | Intermune, Inc. | Method of modulating stress-activated protein kinase system |
US10092555B2 (en) | 2014-06-27 | 2018-10-09 | Celgene Corporation | Compositions and methods for inducing conformational changes in cereblon and other E3 ubiquitin ligases |
USRE47142E1 (en) | 2008-06-03 | 2018-11-27 | Intermune, Inc. | Compounds and methods for treating inflammatory and fibrotic disorders |
US10202384B2 (en) | 2014-09-16 | 2019-02-12 | Gilead Sciences, Inc. | Solid forms of a toll-like receptor modulator |
US10233195B2 (en) | 2014-04-02 | 2019-03-19 | Intermune, Inc. | Anti-fibrotic pyridinones |
US10830762B2 (en) | 2015-12-28 | 2020-11-10 | Celgene Corporation | Compositions and methods for inducing conformational changes in cereblon and other E3 ubiquitin ligases |
US11116774B2 (en) | 2014-07-11 | 2021-09-14 | Gilead Sciences, Inc. | Modulators of toll-like receptors for the treatment of HIV |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6479512B1 (en) * | 1999-10-19 | 2002-11-12 | Merck & Co., Inc. | Tyrosine kinase inhibitors |
US20040097575A1 (en) * | 2002-11-08 | 2004-05-20 | Doherty James B. | Ophthalmic compositions for treating ocular hypertension |
Family Cites Families (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2534580A1 (en) * | 1982-10-13 | 1984-04-20 | Synthelabo | PHENYL-1 PIPERIDINO-2 PROPANOL DERIVATIVES, THEIR PREPARATION, AND MEDICINES THAT CONTAIN THEM |
US5151444B1 (en) * | 1987-09-18 | 1999-07-06 | R Tech Ueno Ltd | Ocular hypotensive agents |
US5296504A (en) * | 1988-09-06 | 1994-03-22 | Kabi Pharmacia | Prostaglandin derivatives for the treatment of glaucoma or ocular hypertension |
EP0569046B1 (en) * | 1988-09-06 | 2002-11-13 | Pharmacia Aktiebolag | Prostaglandin derivatives for the treatment of glaucoma or ocular hypertension |
US5352708A (en) * | 1992-09-21 | 1994-10-04 | Allergan, Inc. | Non-acidic cyclopentane heptanoic acid, 2-cycloalkyl or arylalkyl derivatives as therapeutic agents |
US5510383A (en) * | 1993-08-03 | 1996-04-23 | Alcon Laboratories, Inc. | Use of cloprostenol, fluprostenol and their salts and esters to treat glaucoma and ocular hypertension |
US5573758A (en) * | 1995-04-28 | 1996-11-12 | Allergan | Method for reducing intraocular pressure in the mammalian eye by administration of potassium channel blockers |
US5925342A (en) * | 1996-11-13 | 1999-07-20 | Allergan | Method for reducing intraocular pressure in the mammalian eye by administration of potassium channel blockers |
-
2007
- 2007-03-09 JP JP2009500400A patent/JP2009533326A/en not_active Withdrawn
- 2007-03-09 WO PCT/US2007/006109 patent/WO2007108968A2/en active Application Filing
- 2007-03-09 EP EP07752786A patent/EP2004193A2/en not_active Withdrawn
- 2007-03-09 CA CA002644716A patent/CA2644716A1/en not_active Abandoned
- 2007-03-09 US US12/225,026 patent/US20090062280A1/en not_active Abandoned
- 2007-03-09 AU AU2007227664A patent/AU2007227664A1/en not_active Abandoned
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6479512B1 (en) * | 1999-10-19 | 2002-11-12 | Merck & Co., Inc. | Tyrosine kinase inhibitors |
US20040097575A1 (en) * | 2002-11-08 | 2004-05-20 | Doherty James B. | Ophthalmic compositions for treating ocular hypertension |
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Also Published As
Publication number | Publication date |
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US20090062280A1 (en) | 2009-03-05 |
JP2009533326A (en) | 2009-09-17 |
EP2004193A2 (en) | 2008-12-24 |
WO2007108968A3 (en) | 2007-11-29 |
AU2007227664A1 (en) | 2007-09-27 |
CA2644716A1 (en) | 2007-09-27 |
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