WO2007108689A2 - Nouveau procede d'induction de la differenciation de cellules souches et progenitrices - Google Patents

Nouveau procede d'induction de la differenciation de cellules souches et progenitrices Download PDF

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Publication number
WO2007108689A2
WO2007108689A2 PCT/NL2007/050120 NL2007050120W WO2007108689A2 WO 2007108689 A2 WO2007108689 A2 WO 2007108689A2 NL 2007050120 W NL2007050120 W NL 2007050120W WO 2007108689 A2 WO2007108689 A2 WO 2007108689A2
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WIPO (PCT)
Prior art keywords
cells
differentiation
stem cells
polyamine
progenitor
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PCT/NL2007/050120
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English (en)
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WO2007108689A3 (fr
Inventor
Martinus Nicolaas Helder
Paulus Ignatius Jozef Maria Wuisman
Behrouz Zandieh Doulabi
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Stichting Skeletal Tissue Engineering
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Application filed by Stichting Skeletal Tissue Engineering filed Critical Stichting Skeletal Tissue Engineering
Publication of WO2007108689A2 publication Critical patent/WO2007108689A2/fr
Publication of WO2007108689A3 publication Critical patent/WO2007108689A3/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0655—Chondrocytes; Cartilage
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0654—Osteocytes, Osteoblasts, Odontocytes; Bones, Teeth
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/38—Vitamins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/46—Amines, e.g. putrescine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/155—Bone morphogenic proteins [BMP]; Osteogenins; Osteogenic factor; Bone inducing factor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/13—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells
    • C12N2506/1346—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells from mesenchymal stem cells
    • C12N2506/1384—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells from mesenchymal stem cells from adipose-derived stem cells [ADSC], from adipose stromal stem cells

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Rheumatology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Orthopedic Medicine & Surgery (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)

Abstract

La présente invention concerne la propagation et la différenciation des cellules souches et les stimuli chimiques qui sont utilisés dans l'environnement de ces cellules pour induire la différenciation. Plus particulièrement, l'invention concerne un procédé d'induction ex vivo de la différenciation de cellules souches mésenchymateuses, dérivées de préférence de tissu adipeux, ou de cellules progénitrices en ajoutant une polyamine aux cellules pendant une courte durée (1 min - 2 heures), puis en l'éliminant. D'autres facteurs d'induction de la différenciation peuvent être ajoutés en parallèle, ces facteurs appartenant de préférence au groupe comrenant essentiellement des hormones stéroïdiennes, des vitamines et/ou des hormones de croissance, la LMP-I et leurs combinaisons. Le procédé décrit assure préférentiellement une différenciation ostéogénique ou chondrogénique et les cellules sont donc combinées de préférence à des matières bio-résorbables telles que des phosphates de calcium ostéo-conducteurs, des polymères bio-résorbables tels que des polylactides ou des polyglycolides, des poly-caprolactones, des collagènes, des minéraux, des fibrinogènes, des alginates, des hyaluronases, du chitosan et/ou leurs combinaisons. Les cellules souches et/ou les matières bio-résorbables seront soit implantées directement, soit davantage cultivées dans un milieu de culture approprié avant implantation. De plus, l'utilisation d'une polyamine est décrite en tant que procédé permettant d'éviter la dé-différenciation des cellules et/ou d'induire la trans-différenciation des cellules.
PCT/NL2007/050120 2006-03-21 2007-03-21 Nouveau procede d'induction de la differenciation de cellules souches et progenitrices WO2007108689A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP06075658.2 2006-03-21
EP06075658 2006-03-21

Publications (2)

Publication Number Publication Date
WO2007108689A2 true WO2007108689A2 (fr) 2007-09-27
WO2007108689A3 WO2007108689A3 (fr) 2007-11-15

Family

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Family Applications (1)

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PCT/NL2007/050120 WO2007108689A2 (fr) 2006-03-21 2007-03-21 Nouveau procede d'induction de la differenciation de cellules souches et progenitrices

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WO (1) WO2007108689A2 (fr)

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20100119492A1 (en) * 2008-10-31 2010-05-13 Synthes Usa, Llc Method and device for activating stem cells
WO2011144785A1 (fr) 2010-05-18 2011-11-24 Universidade De Santiago De Compostela Utilisation de poloxamines comme inductrices de la différenciation ostéogénique de cellules mésenchymateuses
CN104877962A (zh) * 2015-04-15 2015-09-02 广州赛莱拉干细胞科技股份有限公司 一种无血清的脂肪干细胞培养基及其制备方法
CN104877963A (zh) * 2015-04-15 2015-09-02 广州赛莱拉干细胞科技股份有限公司 一种无血清的人脐带间充质干细胞培养基及其制备方法
CN104877961A (zh) * 2015-04-15 2015-09-02 广州赛莱拉干细胞科技股份有限公司 一种无血清的人羊膜间充质干细胞培养基及其制备方法
EP2843042A4 (fr) * 2012-04-25 2015-12-30 Riken Préparation cellulaire contenant une cellule engagée du myocarde
CN110832321A (zh) * 2017-06-29 2020-02-21 K·埃瑟 鉴定诱导未分化或去分化的实体肿瘤细胞(再)分化的制剂的方法

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996040866A1 (fr) * 1995-06-07 1996-12-19 Novartis Ag Milieux sans serum pour des cellules hematopoietiques primaires et procedes d'utilisation
WO2003011277A2 (fr) * 2001-07-31 2003-02-13 Anormed Inc. Methodes de mobilisation de cellules souches/embryonnaires
EP1332673A1 (fr) * 1998-02-17 2003-08-06 Gamida Cell Ltd. Procédé de contrôler la prolifération et la différentiation de cellules souches et progenitrices
WO2005034625A1 (fr) * 2003-09-12 2005-04-21 Becton, Dickinson And Company Collagene vi fixe par covalence pour fixation et proliferation de cellules

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996040866A1 (fr) * 1995-06-07 1996-12-19 Novartis Ag Milieux sans serum pour des cellules hematopoietiques primaires et procedes d'utilisation
EP1332673A1 (fr) * 1998-02-17 2003-08-06 Gamida Cell Ltd. Procédé de contrôler la prolifération et la différentiation de cellules souches et progenitrices
WO2003011277A2 (fr) * 2001-07-31 2003-02-13 Anormed Inc. Methodes de mobilisation de cellules souches/embryonnaires
WO2005034625A1 (fr) * 2003-09-12 2005-04-21 Becton, Dickinson And Company Collagene vi fixe par covalence pour fixation et proliferation de cellules

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
AMEMIYA K ET AL: "Adult human retinal pigment epithelial cells capable of differentiating into neurons" BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, ACADEMIC PRESS INC. ORLANDO, FL, US, vol. 316, no. 1, 26 March 2004 (2004-03-26), pages 1-5, XP004493132 ISSN: 0006-291X *
KNIPPENBERG ET AL: "Osteogenesis versus chondrogenesis by BMP-2 and BMP-7 in adipose stem cells" BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, ACADEMIC PRESS INC. ORLANDO, FL, US, vol. 342, no. 3, 2006, pages 902-908, XP005312603 ISSN: 0006-291X *
MAEDA TOMOJI ET AL: "Role of polyamines derived from arginine in differentiation and proliferation of human blood cells." BIOLOGICAL & PHARMACEUTICAL BULLETIN FEB 2006, vol. 29, no. 2, February 2006 (2006-02), pages 234-239, XP002445025 ISSN: 0918-6158 *
MURASHOV A K ET AL: "17beta-Estradiol enhances neuronal differentiation of mouse embryonic stem cells" FEBS LETTERS, ELSEVIER, AMSTERDAM, NL, vol. 569, no. 1-3, 2 July 2004 (2004-07-02), pages 165-168, XP004517478 ISSN: 0014-5793 *
TAKANO T ET AL: "Role of polyamines in expression of the differentiated phenotype of chondrocytes: effect of DL-alpha-hydrazino-delta-aminovaleric acid (DL-HAVA), an inhibitor of ornithine decarboxylase, on chondrocytes treated with parathyroid hormone." JOURNAL OF BIOCHEMISTRY FEB 1983, vol. 93, no. 2, February 1983 (1983-02), pages 591-598, XP002445026 ISSN: 0021-924X *

Cited By (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20100119492A1 (en) * 2008-10-31 2010-05-13 Synthes Usa, Llc Method and device for activating stem cells
US20160222351A1 (en) * 2008-10-31 2016-08-04 DePuy Synthes Products, Inc. Method and Device for Activating Stem Cells
US10494604B2 (en) 2008-10-31 2019-12-03 DePuy Synthes Products, Inc. Method and device for activating stem cells
WO2011144785A1 (fr) 2010-05-18 2011-11-24 Universidade De Santiago De Compostela Utilisation de poloxamines comme inductrices de la différenciation ostéogénique de cellules mésenchymateuses
EP2843042A4 (fr) * 2012-04-25 2015-12-30 Riken Préparation cellulaire contenant une cellule engagée du myocarde
US9644181B2 (en) 2012-04-25 2017-05-09 Riken Cell preparation containing myocardium-committed cell
CN104877962A (zh) * 2015-04-15 2015-09-02 广州赛莱拉干细胞科技股份有限公司 一种无血清的脂肪干细胞培养基及其制备方法
CN104877963A (zh) * 2015-04-15 2015-09-02 广州赛莱拉干细胞科技股份有限公司 一种无血清的人脐带间充质干细胞培养基及其制备方法
CN104877961A (zh) * 2015-04-15 2015-09-02 广州赛莱拉干细胞科技股份有限公司 一种无血清的人羊膜间充质干细胞培养基及其制备方法
CN110832321A (zh) * 2017-06-29 2020-02-21 K·埃瑟 鉴定诱导未分化或去分化的实体肿瘤细胞(再)分化的制剂的方法

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Publication number Publication date
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