WO2007107375A3 - Glass pipette or glass capillary for patch clamp experiments - Google Patents

Glass pipette or glass capillary for patch clamp experiments Download PDF

Info

Publication number
WO2007107375A3
WO2007107375A3 PCT/EP2007/002587 EP2007002587W WO2007107375A3 WO 2007107375 A3 WO2007107375 A3 WO 2007107375A3 EP 2007002587 W EP2007002587 W EP 2007002587W WO 2007107375 A3 WO2007107375 A3 WO 2007107375A3
Authority
WO
WIPO (PCT)
Prior art keywords
glass
patch clamp
pipette
capillary
clamp experiments
Prior art date
Application number
PCT/EP2007/002587
Other languages
German (de)
French (fr)
Other versions
WO2007107375A2 (en
Inventor
Albrecht Lepple-Wienhues
Original Assignee
Flyion Gmbh
Albrecht Lepple-Wienhues
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Flyion Gmbh, Albrecht Lepple-Wienhues filed Critical Flyion Gmbh
Publication of WO2007107375A2 publication Critical patent/WO2007107375A2/en
Publication of WO2007107375A3 publication Critical patent/WO2007107375A3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/48707—Physical analysis of biological material of liquid biological material by electrical means
    • G01N33/48728—Investigating individual cells, e.g. by patch clamp, voltage clamp
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Biochemistry (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Devices For Use In Laboratory Experiments (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The invention especially relates to a glass pipette or a glass capillary (1) for patch clamp experiments, comprising a conical tip (2) and an essentially tubular section (3) adjacent to the tip (2). Said pipette or capillary is characterised in that the diameter thereof extends from a diameter D1 at the base surface of the tip, of less than 50 µm, to a diameter D2 of at least 100 µm in the tubular section, over a length L of less than 150 µm. The invention also relates to the use of such a pipette or capillary for carrying out patch clamp experiments, and to patch clamp measurements using such pipettes or capillaries.
PCT/EP2007/002587 2006-03-23 2007-03-23 Glass pipette or glass capillary for patch clamp experiments WO2007107375A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE102006014513.5 2006-03-23
DE200610014513 DE102006014513A1 (en) 2006-03-23 2006-03-23 Glass pipette or glass capillary for patch clamp experiments

Publications (2)

Publication Number Publication Date
WO2007107375A2 WO2007107375A2 (en) 2007-09-27
WO2007107375A3 true WO2007107375A3 (en) 2007-11-22

Family

ID=38426481

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2007/002587 WO2007107375A2 (en) 2006-03-23 2007-03-23 Glass pipette or glass capillary for patch clamp experiments

Country Status (2)

Country Link
DE (1) DE102006014513A1 (en)
WO (1) WO2007107375A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2710109B1 (en) 2011-05-19 2017-10-25 NMI Naturwissenschaftliches und Medizinisches Institut an der Universität Tübingen Method and utilization of a device for automatically determining the position of a microsystem for manipulating a spherical micro-object

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001048475A1 (en) * 1999-12-24 2001-07-05 Astrazeneca Ab Apparatus for and method of making electrical measurements on an object
WO2002010747A2 (en) * 2000-07-31 2002-02-07 Flyion Gmbh Method and apparatus for patch-clamp measurements on cells

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001048475A1 (en) * 1999-12-24 2001-07-05 Astrazeneca Ab Apparatus for and method of making electrical measurements on an object
WO2002010747A2 (en) * 2000-07-31 2002-02-07 Flyion Gmbh Method and apparatus for patch-clamp measurements on cells

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
HAMILL O P ET AL: "IMPROVED PATCH-CLAMP TECHNIQUES FOR HIGH-RESOLUTION CURRENT RECORDING FROM CELLS AND CELL-FREE MEMBRANE PATCHES", PFLUEGERS ARCHIV: EUROPEAN JOURNAL OF PHYSIOLOGY, SPRINGER VERLAG, BERLIN, DE, vol. 391, 1981, pages 85 - 100, XP000196663, ISSN: 0031-6768 *
NEHER E ET AL: "THE EXTRACELLULAR PATCH CLAMP: A METHOD FOR RESOLVING CURRENT THROUGH INDIVIDUAL OPEN CHANNELS IN BIOLOGICAL MEMBRANES", PFLUEGERS ARCHIV: EUROPEAN JOURNAL OF PHYSIOLOGY, SPRINGER VERLAG, BERLIN, DE, vol. 375, no. 2, 1978, pages 219 - 228, XP009075888, ISSN: 0031-6768 *

Also Published As

Publication number Publication date
WO2007107375A2 (en) 2007-09-27
DE102006014513A1 (en) 2007-09-27

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