WO2007102140A3 - A semliki forest virus replication competent vector with enhanced biosafety - Google Patents

A semliki forest virus replication competent vector with enhanced biosafety Download PDF

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Publication number
WO2007102140A3
WO2007102140A3 PCT/IE2007/000031 IE2007000031W WO2007102140A3 WO 2007102140 A3 WO2007102140 A3 WO 2007102140A3 IE 2007000031 W IE2007000031 W IE 2007000031W WO 2007102140 A3 WO2007102140 A3 WO 2007102140A3
Authority
WO
WIPO (PCT)
Prior art keywords
virus replication
replication competent
forest virus
semliki forest
vector
Prior art date
Application number
PCT/IE2007/000031
Other languages
French (fr)
Other versions
WO2007102140A2 (en
Inventor
Sareen Elizabeth Galbraith
Gregory Julian Atkins
Brian Sheahan
Original Assignee
Trinity College Dublin
Univ Dublin
Sareen Elizabeth Galbraith
Gregory Julian Atkins
Brian Sheahan
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Trinity College Dublin, Univ Dublin, Sareen Elizabeth Galbraith, Gregory Julian Atkins, Brian Sheahan filed Critical Trinity College Dublin
Publication of WO2007102140A2 publication Critical patent/WO2007102140A2/en
Publication of WO2007102140A3 publication Critical patent/WO2007102140A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/13—Tumour cells, irrespective of tissue of origin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011—Details
    • C12N2770/36011—Togaviridae
    • C12N2770/36111—Alphavirus, e.g. Sindbis virus, VEE, EEE, WEE, Semliki
    • C12N2770/36141—Use of virus, viral particle or viral elements as a vector
    • C12N2770/36143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Virology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The invention provides an RNA vector cassette comprising a subgenomic promoter and multiple cloning site (MCS) at the 5' end of the open reading frame of a gene encoding a SFV structural protein. The invention also provides for a replicating viral vector capable of expressing a cloned exogenous gene, wherein the vector demonstrates reduced virulence due to at least one mutation in the coding sequence of a structural protein of a virus. The mutation may be a deletion in the gene.
PCT/IE2007/000031 2006-03-08 2007-03-07 A semliki forest virus replication competent vector with enhanced biosafety WO2007102140A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IE20060173 2006-03-08
IE2006/0173 2006-03-08

Publications (2)

Publication Number Publication Date
WO2007102140A2 WO2007102140A2 (en) 2007-09-13
WO2007102140A3 true WO2007102140A3 (en) 2007-11-22

Family

ID=38441969

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IE2007/000031 WO2007102140A2 (en) 2006-03-08 2007-03-07 A semliki forest virus replication competent vector with enhanced biosafety

Country Status (1)

Country Link
WO (1) WO2007102140A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012001196A2 (en) * 2010-06-28 2012-01-05 Proyecto De Biomedicina Cima, S.L. Alphaviral vectors and the uses thereof for heterologous gene expression
US9840703B2 (en) 2013-01-11 2017-12-12 Children's Medical Center Corporation Methods and compositions for the production of siRNAs

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003104468A1 (en) * 2002-06-10 2003-12-18 The Provost, Fellows And Scholars Of The College Of The Holy And Undivided Trinity Of Queen Elizabeth Near Dublin A semliki forest virus based expression vector for the central nervous system

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003104468A1 (en) * 2002-06-10 2003-12-18 The Provost, Fellows And Scholars Of The College Of The Holy And Undivided Trinity Of Queen Elizabeth Near Dublin A semliki forest virus based expression vector for the central nervous system

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
GALBRAITH SAREEN E ET AL: "Deletions in the hypervariable domain of the nsP3 gene attenuate Semliki Forest virus virulence", JOURNAL OF GENERAL VIROLOGY, vol. 87, no. Part 4, April 2006 (2006-04-01), pages 937 - 947, XP002449615, ISSN: 0022-1317 *
MCINERNEY G M ET AL: "Semliki Forest virus produced in the absence of the 6K protein has an altered spike structure as revealed by decreased membrane fusion capacity", VIROLOGY, ACADEMIC PRESS,ORLANDO, US, vol. 325, no. 2, 1 August 2004 (2004-08-01), pages 200 - 206, XP004520306, ISSN: 0042-6822 *
PIERRO D J ET AL: "Development of an orally infectious Sindbis virus transducing system that efficiently disseminates and expresses green fluorescent protein in Aedes aegypti.", INSECT MOLECULAR BIOLOGY, vol. 12, no. 2, April 2003 (2003-04-01), pages 107 - 116, XP002449613, ISSN: 0962-1075 *
TUITTILA MINNA T ET AL: "Replicase complex genes of Semliki Forest virus confer lethal neurovirulence", JOURNAL OF VIROLOGY, vol. 74, no. 10, May 2000 (2000-05-01), pages 4579 - 4589, XP002449692, ISSN: 0022-538X *
VAHA-KOSKELA M J V ET AL: "A NOVEL NEUROTROPIC EXPRESSION VECTOR BASED ON THE AVIRULENT A7(74) STRAIN OF SEMLIKI FOREST VIRUS", JOURNAL OF NEUROVIROLOGY, BASINGSTOKE, GB, vol. 9, no. 1, February 2003 (2003-02-01), pages 1 - 15, XP009016060, ISSN: 1355-0284 *
VIHINEN HELENA ET AL: "Elimination of phosphorylation sites of Semliki Forest virus replicase protein nsP3", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 276, no. 8, 23 February 2001 (2001-02-23), pages 5745 - 5752, XP002449612, ISSN: 0021-9258 *

Also Published As

Publication number Publication date
WO2007102140A2 (en) 2007-09-13

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