WO2007075807A3 - Methods for the directed differentiation of embryonic stem cell - Google Patents

Methods for the directed differentiation of embryonic stem cell Download PDF

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Publication number
WO2007075807A3
WO2007075807A3 PCT/US2006/048641 US2006048641W WO2007075807A3 WO 2007075807 A3 WO2007075807 A3 WO 2007075807A3 US 2006048641 W US2006048641 W US 2006048641W WO 2007075807 A3 WO2007075807 A3 WO 2007075807A3
Authority
WO
WIPO (PCT)
Prior art keywords
methods
embryonic stem
directed differentiation
stem cell
cells
Prior art date
Application number
PCT/US2006/048641
Other languages
French (fr)
Other versions
WO2007075807A2 (en
WO2007075807A9 (en
Inventor
William Lathrop Rust
Alan Colman
William Sun
Norris Ray Dunn
Blaine Phillips
Hentze Hannes Martin
Original Assignee
Es Cell Int Pte Ltd
William Lathrop Rust
Alan Colman
William Sun
Norris Ray Dunn
Blaine Phillips
Hentze Hannes Martin
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Es Cell Int Pte Ltd, William Lathrop Rust, Alan Colman, William Sun, Norris Ray Dunn, Blaine Phillips, Hentze Hannes Martin filed Critical Es Cell Int Pte Ltd
Publication of WO2007075807A2 publication Critical patent/WO2007075807A2/en
Publication of WO2007075807A9 publication Critical patent/WO2007075807A9/en
Publication of WO2007075807A3 publication Critical patent/WO2007075807A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0676—Pancreatic cells
    • C12N5/0677—Three-dimensional culture, tissue culture or organ culture; Encapsulated cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/30—Synthetic polymers
    • C12N2533/40—Polyhydroxyacids, e.g. polymers of glycolic or lactic acid (PGA, PLA, PLGA); Bioresorbable polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/54—Collagen; Gelatin

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • Reproductive Health (AREA)
  • Developmental Biology & Embryology (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Gynecology & Obstetrics (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The present invention provides compositions and methods for the directed differentiation of embryonic stem cells. The invention further provides the use of cells differentiated from the ES cells for treating diseases and conditions.
PCT/US2006/048641 2005-12-20 2006-12-20 Methods for the directed differentiation of embryonic stem cell WO2007075807A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US75221405P 2005-12-20 2005-12-20
US60/752,214 2005-12-20

Publications (3)

Publication Number Publication Date
WO2007075807A2 WO2007075807A2 (en) 2007-07-05
WO2007075807A9 WO2007075807A9 (en) 2007-08-16
WO2007075807A3 true WO2007075807A3 (en) 2008-01-31

Family

ID=38218573

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2006/048641 WO2007075807A2 (en) 2005-12-20 2006-12-20 Methods for the directed differentiation of embryonic stem cell

Country Status (1)

Country Link
WO (1) WO2007075807A2 (en)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7473555B2 (en) 2000-04-27 2009-01-06 Geron Corporation Protocols for making hepatocytes from embryonic stem cells
EP1463798A4 (en) 2001-12-07 2005-01-19 Geron Corp Islet cells from human embryonic stem cells
US7695965B2 (en) 2006-03-02 2010-04-13 Cythera, Inc. Methods of producing pancreatic hormones
US8148151B2 (en) 2006-06-02 2012-04-03 Geron Corporation Differentiation of primate pluripotent cells to hepatocyte-lineage cells
CA2695288A1 (en) 2007-08-01 2009-02-05 Ethicon, Inc. Collagen-related peptides and uses thereof
SG154367A1 (en) * 2008-01-31 2009-08-28 Es Cell Int Pte Ltd Method of differentiating stem cells
US20100021527A1 (en) 2008-07-25 2010-01-28 Chunlin Yang Collagen-related peptides and uses thereof and hemostatic foam substrates
CN102370611B (en) * 2010-08-17 2013-09-18 东莞太力生物工程有限公司 Temperature-sensitive hydrogel containing exendin-4 and injection thereof
US9404087B2 (en) 2010-12-15 2016-08-02 Kadimastem Ltd. Insulin producing cells derived from pluripotent stem cells
KR102205234B1 (en) 2013-06-11 2021-01-20 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 SC-β CELLS AND COMPOSITIONS AND METHODS FOR GENERATING THE SAME
DK3234110T3 (en) 2014-12-18 2024-05-13 Harvard College METHODS OF GENERATION OF STEM CELL-DERIVED ß-CELLS AND USES THEREOF
US10443042B2 (en) 2014-12-18 2019-10-15 President And Fellows Of Harvard College Serum-free in vitro directed differentiation protocol for generating stem cell-derived beta cells and uses thereof
CN107614678B (en) 2014-12-18 2021-04-30 哈佛学院校长同事会 Method for producing stem cell-derived beta cells and method for using same
CN105112367B (en) * 2015-08-28 2018-07-27 青岛瑞思科生物科技有限公司 A kind of mescenchymal stem cell epidermal differentiation derivant and its application process
WO2017073761A1 (en) * 2015-10-28 2017-05-04 学校法人近畿大学 Method for forming embryoid body of pluripotent stem cells and composition for forming embryoid body of pluripotent stem cells
IL274436B2 (en) 2017-11-15 2024-01-01 Semma Therapeutics Inc Islet cell manufacturing compositions and methods of use
EP3833365A4 (en) 2018-08-10 2022-05-11 Vertex Pharmaceuticals Incorporated Stem cell derived islet differentiation

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004104184A1 (en) * 2003-05-20 2004-12-02 Riken Preparation of endodermal stem cells

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004104184A1 (en) * 2003-05-20 2004-12-02 Riken Preparation of endodermal stem cells
EP1627912A1 (en) * 2003-05-20 2006-02-22 Riken Preparation of endodermal stem cells

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
KAHAN B W ET AL: "Pancreatic precursors and differentiated islet cell types from murine embryonic stem cells: An in vitro model to study islet differentiation", DIABETES, NEW YORK, NY, US, vol. 52, no. 8, August 2003 (2003-08-01), pages 2016 - 2024, XP002365297, ISSN: 0012-1797 *
KLEINMAN ET AL: "Matrigel: Basement membrane matrix with biological activity", SEMINARS IN CANCER BIOLOGY, SAUNDERS SCIENTIFIC PUBLICATIONS, PHILADELPHIA, PA, US, vol. 15, no. 5, October 2005 (2005-10-01), pages 378 - 386, XP005021947, ISSN: 1044-579X *
KUBO A ET AL: "Development of definitive endoderm from embryonic stem cells in culture", DEVELOPMENT, COMPANY OF BIOLOGISTS, CAMBRIDGE,, GB, vol. 131, no. 7, April 2004 (2004-04-01), pages 1651 - 1662, XP002985523, ISSN: 0950-1991 *
LEE JUNG BOK ET AL: "Available human feeder cells for the maintenance of human embryonic stem cells", REPRODUCTION, PORTLAND PRESS,, AU, vol. 128, no. 6, December 2004 (2004-12-01), pages 727 - 735, XP002377143, ISSN: 1470-1626 *
LEVENBERG S ET AL: "Endothelial cells derived from human embryonic stem cells", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, NATIONAL ACADEMY OF SCIENCE, WASHINGTON, DC, US, vol. 99, no. 7, 2 April 2002 (2002-04-02), pages 4391 - 4396, XP002980825, ISSN: 0027-8424 *
TADA S ET AL: "CHARACTERIZATION OF MESENDODERM: A DIVERGING POINT OF THE DEFINITIVE ENDODERM AND MESODERM IN EMBRYONIC STEM CELL DIFFERENTIATION CULTURE", DEVELOPMENT, COMPANY OF BIOLOGISTS, CAMBRIDGE,, GB, vol. 132, no. 19, October 2005 (2005-10-01), pages 4363 - 4374, XP008054294, ISSN: 0950-1991 *

Also Published As

Publication number Publication date
WO2007075807A2 (en) 2007-07-05
WO2007075807A9 (en) 2007-08-16

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