WO2007075456A3 - Colorimetric light switching probe for ultrasensitive detection of target compounds - Google Patents

Colorimetric light switching probe for ultrasensitive detection of target compounds Download PDF

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Publication number
WO2007075456A3
WO2007075456A3 PCT/US2006/047988 US2006047988W WO2007075456A3 WO 2007075456 A3 WO2007075456 A3 WO 2007075456A3 US 2006047988 W US2006047988 W US 2006047988W WO 2007075456 A3 WO2007075456 A3 WO 2007075456A3
Authority
WO
WIPO (PCT)
Prior art keywords
probe
target compounds
light switching
ultrasensitive detection
emission
Prior art date
Application number
PCT/US2006/047988
Other languages
French (fr)
Other versions
WO2007075456A2 (en
Inventor
Weihong Tan
Chaoyong Yang
Marie C Vicens
Nicholas J Turro
Steffen Jockusch
Original Assignee
Univ Florida
Univ Columbia
Weihong Tan
Chaoyong Yang
Marie C Vicens
Nicholas J Turro
Steffen Jockusch
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Florida, Univ Columbia, Weihong Tan, Chaoyong Yang, Marie C Vicens, Nicholas J Turro, Steffen Jockusch filed Critical Univ Florida
Publication of WO2007075456A2 publication Critical patent/WO2007075456A2/en
Publication of WO2007075456A3 publication Critical patent/WO2007075456A3/en

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5308—Immunoassay; Biospecific binding assay; Materials therefor for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
    • G01N33/57484—Immunoassay; Biospecific binding assay; Materials therefor for cancer involving compounds serving as markers for tumor, cancer, neoplasia, e.g. cellular determinants, receptors, heat shock/stress proteins, A-protein, oligosaccharides, metabolites
    • G01N33/57488—Immunoassay; Biospecific binding assay; Materials therefor for cancer involving compounds serving as markers for tumor, cancer, neoplasia, e.g. cellular determinants, receptors, heat shock/stress proteins, A-protein, oligosaccharides, metabolites involving compounds identifable in body fluids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/475—Assays involving growth factors
    • G01N2333/49—Platelet-derived growth factor [PDGF]

Abstract

A system and method for detecting a target compound, comprising an aptamer having a high affinity for the target compound, wherein two labels are attached to the aptamer. The two labels cause the probe to emit a baseline, non-visible emission. When the aptamer (also referred to herein as a probe) of the invention interacts with a target compound, the probe undergoes a conformational change, causing the fluorescence emission to change from the baseline emission to an emission that is detectable.
PCT/US2006/047988 2005-12-15 2006-12-15 Colorimetric light switching probe for ultrasensitive detection of target compounds WO2007075456A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US75084205P 2005-12-15 2005-12-15
US60/750,842 2005-12-15

Publications (2)

Publication Number Publication Date
WO2007075456A2 WO2007075456A2 (en) 2007-07-05
WO2007075456A3 true WO2007075456A3 (en) 2007-11-01

Family

ID=38218475

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2006/047988 WO2007075456A2 (en) 2005-12-15 2006-12-15 Colorimetric light switching probe for ultrasensitive detection of target compounds

Country Status (1)

Country Link
WO (1) WO2007075456A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108646014B (en) * 2018-05-21 2020-07-17 青岛大学 Method for fluorescence detection of platelet-derived growth factor based on aptamer conformational change
CN113548981B (en) * 2021-08-05 2023-04-28 齐鲁工业大学 Triphenylamine phenol compound, preparation method thereof and application thereof in detection of tryptophan
CN113945720A (en) * 2021-09-26 2022-01-18 瑞博奥(广州)生物科技股份有限公司 PDGF-BB recognition method based on aptamer probe and PDGF-BB detection kit

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040067503A1 (en) * 2002-04-22 2004-04-08 Weihong Tan Functionalized nanoparticles and methods of use

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040067503A1 (en) * 2002-04-22 2004-04-08 Weihong Tan Functionalized nanoparticles and methods of use

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
LI J.J. ET AL.: "Molecular aptamer beacons for real-time protein recognition", BIOCHEM. BIOPHYS. RES. COMMUN., vol. 292, no. 1, 2002, pages 31 - 40, XP002986372 *
LUZI E. ET AL.: "New trends in affinity sensing: aptamers for ligand binding", TRENDS IN ANAL. CHEM., vol. 22, no. 11, 2003, pages 810 - 818, XP002374941 *
TAN W. ET AL.: "Molecular Beacons", CURR. OPIN. CHEM. BIOL., vol. 8, 2004, pages 547 - 553, XP004576568 *
YAMAMOTO R. ET AL.: "Molecular beacon aptamer fluorescens in the presence of Tat protein of HIV-1", GENES TO CELLS, vol. 5, no. 5, 2000, pages 389 - 396, XP002224741 *
YANG C.-J. ET AL.: "Light-switching excimer probes for rapid protein monitoring in complex biological fluids", PROC. NATL. ACAD. SCI. USA, vol. 102, no. 48, 29 November 2005 (2005-11-29), pages 17278 - 17283 *

Also Published As

Publication number Publication date
WO2007075456A2 (en) 2007-07-05

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