WO2007071927A2 - Complexes et colorants fluorescents - Google Patents

Complexes et colorants fluorescents Download PDF

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Publication number
WO2007071927A2
WO2007071927A2 PCT/GB2006/004651 GB2006004651W WO2007071927A2 WO 2007071927 A2 WO2007071927 A2 WO 2007071927A2 GB 2006004651 W GB2006004651 W GB 2006004651W WO 2007071927 A2 WO2007071927 A2 WO 2007071927A2
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WO
WIPO (PCT)
Prior art keywords
dye
cell
group
groups
complex
Prior art date
Application number
PCT/GB2006/004651
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English (en)
Other versions
WO2007071927A3 (fr
Inventor
Gerald Arthur Smith
Original Assignee
Cambridge Enterprise Limited
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cambridge Enterprise Limited filed Critical Cambridge Enterprise Limited
Publication of WO2007071927A2 publication Critical patent/WO2007071927A2/fr
Publication of WO2007071927A3 publication Critical patent/WO2007071927A3/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
    • C09B11/00—Diaryl- or thriarylmethane dyes
    • C09B11/04—Diaryl- or thriarylmethane dyes derived from triarylmethanes, i.e. central C-atom is substituted by amino, cyano, alkyl
    • C09B11/06—Hydroxy derivatives of triarylmethanes in which at least one OH group is bound to an aryl nucleus and their ethers or esters
    • C09B11/08—Phthaleins; Phenolphthaleins; Fluorescein
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
    • C09B11/00—Diaryl- or thriarylmethane dyes
    • C09B11/04—Diaryl- or thriarylmethane dyes derived from triarylmethanes, i.e. central C-atom is substituted by amino, cyano, alkyl
    • C09B11/10—Amino derivatives of triarylmethanes
    • C09B11/24—Phthaleins containing amino groups ; Phthalanes; Fluoranes; Phthalides; Rhodamine dyes; Phthaleins having heterocyclic aryl rings; Lactone or lactame forms of triarylmethane dyes
    • C09B11/245—Phthaleins having both OH and amino substituent(s) on aryl ring

Definitions

  • This invention relates to fluorescent dyes comprising a xanthene- derived fluorophore, complexes comprising these dyes, and use of such dyes and complexes in cell and other analysis procedures.
  • Fluorescent dyes when bonded to target molecules such as proteins, are commonly used to probe the properties of living cells.
  • such dyes are often used to probe the pH of individual cell compartments. This is commonly achieved by comparing the intensity of fluorescence of a particular dye when it is within a particular cell compartment with a calibration curve of intensity versus pH for that dye. In this way, information may be obtained about the migration of molecules throughout a cell and/or kinetics of reactions taking place in different compartments of the cell.
  • fluorescein which has the formula
  • the fluorescence response of fluorescein decreases as pH decreases, and while it is useful in providing information on kinetics of reactions taking place within cell compartments, the pH of the cell compartments can not be accurately determined through its use. As a result, it is desirable to provide novel fluorescent dyes with different pH responses, in order to provide a more accurate determination of the internal pH of cell compartments, and for other analysis procedures.
  • a novel fluorescent dye has the formula (I)
  • group X is selected from aliphatic, alicyclic and aromatic amine groups.
  • novel fluorescent dyes have a fluorescence response which increases with pH up to a maximum fluorescence and then decreases as pH continues to increase.
  • the present invention also extends to complexes of the above- described fluorescent dyes with target molecules, and the use of the dyes and complexes to determine and/or monitor the pH of living cells or cell compartments, and in other analysis procedures.
  • the group X may be selected from a wide variety of amine groups, which may be aliphatic, alicyclic or aromatic in nature, or a combination of any of these.
  • the group X is represented by -NR2, in which the R groups are independently selected from hydrogen and alkyl, aryl, aryalkyl and alkryl groups, provided that both R groups are not hydrogen, or where both R groups together form an imine linkage.
  • neither R group is hydrogen.
  • Included in the above definition for the group X are groups -NR 2 which form a ring structure by bonding onto the aryl group of the benzoic acid moiety of the molecule, wherein the ring may be aromatic or non- aromatic in nature.
  • Preferred groups -NR 2 are the dialkylamino groups, in which the alkyl groups may be the same or different, and typically contain up to 10 carbon atoms.
  • a particularly preferred -NR 2 group is a dimethylamino group.
  • One or more of the aryl rings of the fluorescent dyes of the present invention may be optionally substituted, to vary the pH response and/or to aid in bonding, or coupling, to a target molecule or cell analysis procedures.
  • substituents may be included on the aryl rings of the xanthene- derived moiety, as is the case in known fluorescent dye dichlorofluorescein.
  • the fluorescent dye may have the formula (II).
  • X is as defined above and each Y is a halogen atom which may be the same as or different to one another, and is typically selected from chlorine and fluorine.
  • a halogen atom which may be the same as or different to one another, and is typically selected from chlorine and fluorine.
  • One example of such a dye is 2',7'-dichloro-4- (dimethylamino) fluorescein.
  • the fluorescent dyes of the present invention can be prepared by any combination of standard chemical synthetic steps, and in particular those conventionally used in the manufacture of fluorescein and its derivatives.
  • the dyes may be functionalised so that they may be bonded to a range of target molecules, for introduction into a living cell or cell compartment. If desired, a plurality of different dyes, having fluorescence responses at different pH's, may be bonded to a single target molecule. This may be particularly useful for analysing the kinetics of migration of a particular target molecule through different regions of a cell, or through different cell compartments.
  • a living cell includes eukaryotic cells, prokaryotic cells and plant cells. Further, while the present invention is described in the context of use of the novel fluorescent dyes with living cells, the present invention may find use in other applications.
  • bonding groups There is a wide variety of functionalities that may be included in the dyes of the present invention to achieve bonding to target molecules, typically on one or more of the aryl rings, and these are referred to in what follows as “bonding groups".
  • bonding may be achieved by direct reaction between a dye and its target molecule, herein referred to as “direct bonding”.
  • directly bonding may be achieved with the aid of a coupling agent, or “activator”, which activates the dye by forming a complex therewith, and which is typically displaced after reaction with the target molecule.
  • a coupling agent or “activator”
  • a moiety derived therefrom may become incorporated in the final bonded complex.
  • a wide variety of coupling reactions is known in the prior art, and may be applicable to the present invention.
  • the bonding group will depend upon the nature of the functional group(s) on the target molecule available for reaction with the dye.
  • the bonding group may be selected from isocyanates; isothiocyanates; and carbonyl groups containing a leaving group, for instance acid chloride, sulphonate, carboxylate, and so-called "active" esters, i.e. containing very good leaving groups including, for instance, nitrophenyl and N-hydroxysuccinimide, all of which are capable of direct bonding to said amino or amine groups on the target molecule.
  • the bonding group may comprise a carboxylic acid group, an aldehyde or a ketone which, on activation with a coupling agent, are capable of reaction with said amino or amine groups.
  • the bonding group is an aldehyde or ketone, the reaction is typically conducted under reducing conditions to give rise to an amino-alkyl chain linkage in the final dye/target molecule complex.
  • the bonding group may be selected from many of the groups mentioned above, disulphide and thiols.
  • the dyes and complexes of the present invention may be used, either alone or in combination with other dyes or complexes, to establish the pH of a living cell or cell compartment, or to analyse the kinetics of migration of the dyes or complexes into a living cell or cell compartment, or from location to location within a cell.
  • a cell compartment typically we mean one of the many organelles suspended in the cell cytoplasm.
  • the pH of a cell or cell compartment can be measured by introducing a dye or complex into a cell or cell compartment, irradiating the dye or complex with a suitable light source, and observing the intensity of fluorescein of the dye or complex.
  • the observed fluorescence intensity can then be used to determine pH by a variety of methods known in the field, selected according to the method of accumulation of the dye or complex. For instance, the observed fluorescence may be compared to a known standard, for example a calibration curve of fluorescence intensity versus pH, or to fluorescence intensity measurements indicative of the total dye or complex present. Any conventional fluorometric equipment can be used to irradiate the sample, and to measure the resulting fluorescent response.
  • the dyes and complexes are introduced into a living cell or cell compartment by mixing with a sample comprising a cell or cell compartment, and then leaving the mixture to stand for a time interval adequate to allow entry of the dye or complex into the cell or cell compartment. During this time interval, the dye or complex diffuses towards a cell or cell compartment within the sample. The dye or complex then attaches itself to the membrane of a cell or cell compartment.
  • target molecules are generally cell or cell compartment specific, hence a specific complex generally attaches to only one kind of cell or cell compartment.
  • the dye or complex may diffuse through a membrane of that cell or cell compartment or be trafficked to a specific cell compartment by receptor-mediated endocytosis, hence exposing itself to the internal pH of the cell or cell compartment.
  • the dyes may be incorporated into other reporting molecules, and the observed fluorescence used as a measure of a secondary response.
  • use of the dyes of the present invention is not limited to cell analysis procedures. Instead, the dyes find use in a wide variety of direct analysis procedures, for instance where it may be desirable to determine pH or whether the pH of a substance falls within an acceptable range. Examples include analysis of soil or urine.
  • Figure 1 shows the pH titration curve for dichlorofluorescein, and the structure of this molecule is shown to the right of the curve.
  • Figure 2 shows the pH titration curve for 2',7'-dichloro-(4- dimethylamino)fluorescein, which includes a dimethylamino group ortho to the carboxyl group of the benzoic acid moiety, as shown in the formula to the right of the curve. /
  • FIG. 3 shows pH titration curves of fluorescein and three derivatives thereof.
  • Curve A represents dichlorofluorescein
  • curve B fluorescein
  • curve C 2',7'-dichloro-4-(dimethylamino)fluorescein
  • curve D 4-dimethylamino- fluorescein.
  • the titrations were performed at the same molarity and sensitivity settings for each compound.
  • the data show that the pKi value is essentially unaffected by the addition of the dimethylamino substituent, and appears to be dependent solely on the substituents on the xanthene moiety.
  • the value of pK 2 is essentially independent of the substituents on the xanthene moiety.
  • Example 3-(dimethylamino)phthalic acid 3-nitrophthalic acid (10 g) was hydrogenated in ethanol containing 1% acetic acid (200 ml) and 10% Pt on charcoal (500 mg) until uptake of hydrogen was nearly complete (6I).
  • Formaldehyde solution (35%, 20 ml) was added and the hydrogenation continued until hydrogen uptake ceased (ca. 3 I).
  • the catalyst was removed by filtration and washed with aqueous ethanol. Evaporation and filtration gave the required product (4.1 g, 41%).
  • methansulphonic acid is fusion with zinc chloride at approximately 180 0 C for approximately 30 minutes.
  • the diacetate was prepared by dissolution of the free 2',7'-dichloro-4- (dimethylamino)fluorescein (200 mg) in pyridine (2 ml) with excess acetic anhydride (0.5 ml) overnight. The excess anhydride was destroyed by slow addition of water with cooling and the product precipitated with water to 5 ml and collected by filtration, washed with water and dried to give the product (230 mg) as an off-white solid.

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Luminescent Compositions (AREA)
  • Coloring (AREA)
  • Electroluminescent Light Sources (AREA)

Abstract

L’invention concerne un colorant fluorescent de formule (I) : le groupe X étant choisi parmi les groupements amine aromatique, aliphatique et alicyclique.
PCT/GB2006/004651 2005-12-19 2006-12-12 Complexes et colorants fluorescents WO2007071927A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB0525786A GB0525786D0 (en) 2005-12-19 2005-12-19 Fluorescent dyes and complexes
GB0525786.0 2005-12-19

Publications (2)

Publication Number Publication Date
WO2007071927A2 true WO2007071927A2 (fr) 2007-06-28
WO2007071927A3 WO2007071927A3 (fr) 2007-09-07

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PCT/GB2006/004651 WO2007071927A2 (fr) 2005-12-19 2006-12-12 Complexes et colorants fluorescents

Country Status (2)

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GB (1) GB0525786D0 (fr)
WO (1) WO2007071927A2 (fr)

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002055512A1 (fr) * 2001-01-12 2002-07-18 Northeastern University Derives de xanthene
EP1441010A1 (fr) * 1996-04-12 2004-07-28 Molecular Probes, Inc. Dérivés de xanthène fluorés

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1441010A1 (fr) * 1996-04-12 2004-07-28 Molecular Probes, Inc. Dérivés de xanthène fluorés
WO2002055512A1 (fr) * 2001-01-12 2002-07-18 Northeastern University Derives de xanthene

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
B.M. WEIDGANS ET AL: "Fluorescent pH sensors with negligible sensitivity to ionic strength" ANALYST, vol. 129, 2004, pages 645-650, XP002439081 cited in the application *

Also Published As

Publication number Publication date
GB0525786D0 (en) 2006-01-25
WO2007071927A3 (fr) 2007-09-07

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