WO2007069005A1 - Dispositif d’echantillonnage et d’analyse bioluminometrique automatiques - Google Patents

Dispositif d’echantillonnage et d’analyse bioluminometrique automatiques Download PDF

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Publication number
WO2007069005A1
WO2007069005A1 PCT/IB2006/001644 IB2006001644W WO2007069005A1 WO 2007069005 A1 WO2007069005 A1 WO 2007069005A1 IB 2006001644 W IB2006001644 W IB 2006001644W WO 2007069005 A1 WO2007069005 A1 WO 2007069005A1
Authority
WO
WIPO (PCT)
Prior art keywords
fluid
bioluminometric
analyzed
automatizing
analysis
Prior art date
Application number
PCT/IB2006/001644
Other languages
English (en)
Inventor
Giorgio Bellini
Original Assignee
Star Ecotronics S.R.L.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Star Ecotronics S.R.L. filed Critical Star Ecotronics S.R.L.
Priority to EP06765552A priority Critical patent/EP1960755A1/fr
Publication of WO2007069005A1 publication Critical patent/WO2007069005A1/fr

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N35/1004—Cleaning sample transfer devices
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N21/76—Chemiluminescence; Bioluminescence
    • G01N21/763—Bioluminescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/84—Systems specially adapted for particular applications
    • G01N21/88—Investigating the presence of flaws or contamination
    • G01N21/94—Investigating contamination, e.g. dust
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/0099—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor comprising robots or similar manipulators
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N35/1081—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices characterised by the means for relatively moving the transfer device and the containers in an horizontal plane

Definitions

  • the present invention has as an object a device for automatizing fluid sampling and bioluminometric
  • bioluminescence is the most used method to determine sterility or to assess the bacterial charge of a fluid. Such method relies on measuring the amount of light emitted by the reaction occurring when a reagent, namely the luciferin-luciferase, comes into contact with the ATP (adenosine triphosphate) released by the bacteria contained in the fluid being analysed.
  • a reagent namely the luciferin-luciferase
  • the light emission is measured by a photonic count system based on a photo-multiplier and a digital count line.
  • the bacterial charge values of the analysed fluid are adjusted in a second step.
  • a further object of the present invention is to
  • a further object of the present invention is to
  • Another object of the present invention is to produce an automatic fluid sampling and bioluminomet ⁇
  • an object of the present invention is to produce an automatic fluid sampling and bioluminomet- ric analysis device that assesses the bacterial charge of the analyzed fluid in order to compare it with a predetermined minimum threshold value.
  • Fig. 1 shows a front view of the automatic fluid sampling and bioluminometric analysis device accord ⁇
  • FIG. 2 shows a front view of a multifunction recirculation cylinder pertaining to the device according to the present invention.
  • analysis device of the present invention is desig- nated as a whole, in an exemplary embodiment, by the reference number 100.
  • the automatic fluid sampling and bioluminometric analysis device 100 is comprised of a console or cabinet 1 preferably made of steel, which supports the number of components forming the device 100 .
  • fluid sample withdrawing well 4 a container for the frozen reagent vials 5, a variable number of reagent withdrawing wells 6, 6', and a container for sterile tubes 7, from which said tubes are taken away and placed in a rotating tube carrier 20.
  • the head set 200 which is horizontally slidable, also provides a vertically sliding head 51 and a metering syringe 19 on it .
  • a metering syringe slider 53 and a tube gripper 11 are also associated with the handling tower 21.
  • a multifunction rod is associated to the head set 200 too.
  • a container 8 for collecting used tubes is lodged, which container is connected to the console 1 by means of a discharge channel 9 ending at an inlet 10 on which used tubes are dropped by a gripper 11.
  • an intake pipe 12 for the fluid to be ana- lyzed In the underside of the console 1 there are also lodged an intake pipe 12 for the fluid to be ana- lyzed, a discharge pipe 13 for the fluid to be ana- lyzed, a priming pump 14, a pipe for admitting washing fluid 15, a pipe for discharging washing fluid and air 16, a pipe for admitting pressurized air 17, and a tank 18 containing a substance suited for ster- ilization.
  • a washing pulse pump Inside the console 1 are also lodged a washing pulse pump, a number of electrovalves belonging to the hydraulic and pneumatic circuits, and a system
  • the multifunction recirculation cylinder 4 per- taining to the device according to the present invention is shown in fig. 2.
  • an inlet 30 in which a conduit 41 is provided to admit the sterilizing liquid from the tank 18.
  • Said inlet leads to a clear cylinder 36 in which are provided a conduit 40 for the liquid to be analyzed, an upper valve 33 with its own motor 31 and a pre-filter valve 34 with its own motor 32.
  • a filter 35 to block suspended particles is pro-
  • a recirculation valve for the sample to be ana ⁇
  • lyzed is also shown in fig. 2, which valve is shown at positions Hi 38 and Lo 37.
  • the operation of the automatic sampling and bio- luminometric analysis device is the following.
  • a metering syringe 19 is on the handling tower 21 of the head
  • syringe 19 In that position, syringe 19 is enclosed into a sterile room which is protected from dust and inadvertent shocks .
  • the priming pump 14 acting on the sewage dis- charge well 50 and connected to the intake pipe 12 causes continuous circulation of the water to be analyzed.
  • the water continuously passing moves as a whirlpool (which movement is induced by the intake pipe, which is tangential to the compartment body) thus avoiding deposition of solid agglomerates.
  • the pipes are smooth (above all the feed ones) and have a constant caliber to circumvent the same prob ⁇
  • the upper compartment of the cylinder forms the withdrawing well for the sample to be analyzed.
  • the well communicates with the lower compartment of the
  • the withdrawing well When not in use, the withdrawing well has both the conical valve and the upper communication port of the syringe closed.
  • the handling tower 21 places at the multifunction cylinder 4 in order to withdraw the sample to be analyzed.
  • the movement of said tower is performed by an electric motor provided with an adapter and horizon ⁇
  • the position references are absolute, with an acceleration and deceleration motor
  • the multifunction rod extends, opening the up- per plug of the well, and the descending head 51 introduces the metering syringe 19 which, with three- five plunger travels withdraws the sample.
  • the tower 21 is placed at the rotating tube car ⁇
  • the tube carrier 20 rotates 90° so that its inlet is directed towards a communicating ejection hole of the vial container 7.
  • a tube is charged on the rotating carrier 20, which is rotated so that the tube is in a vertical position.
  • the head 51 when going down, introduces the sy- ringe 19 into the tube (the external diameter of the syringe is smaller than the internal diameter of the tube) , and by suitably feeding the motor for a sliding movement of the syringe plunger, a slow ejection
  • the sample is provided.
  • the sampling of the subsequent IOO ⁇ L is per- formed avoiding the multifunction cylinder sample filling first step.
  • reagents contained into compartments 6 and required for the subsequent luminometric analysis are injected into the tube, optionally performing a sterilization and drying step of the syringe 19 after every withdrawal, as needed.
  • a sterilization and drying step of the sy- ringe into the cylinder 4 can be performed, always taking into consideration the reagent permanence times into the tube before the luminometric analysis, which must be constant .
  • the tube is ready for being analyzed.
  • the tower 21 is placed at the location of the rotating tube carrier 20 in order to take the tube by means of the gripper 11. Then, the head 51 rises again taking the tube with it, and the tower 21 moves to the luminometer 2 and the port thereof .
  • the multifunction rod is withdrawn, the port of the luminometer 2 is opened and the multifunction rod enters a hole provided in the insertion carrier. By closing the port the luminometric measurement is automatically started, which allows to assess the sample bacterial concentration.
  • a relays outlet can, if necessary, activate biocide introduc- tion or trigger an alarm indicating the critical threshold.
  • the tower 21 of the head set 51 is placed on the location of the multifunction cylinder 4 and when the work is finished, a complete sterili- zation and cleaning cycle is performed.
  • the described device has highly adaptable characteristics which allows different uses according to the requirements of the analysis one wants to perform.
  • the use of the device can be directed to verify that, in a production process, the bacterial charge of the final product, of one component or of one intermediate product, is below a preset limit. It is specifically provided the opportunity to meter biocides as a corrective action, to provide alarm signals to the operators, and automatically modify the analysis frequency for a more and more ac ⁇
  • the device of the invention can be used to control a bacterial presence being greater than a minimum amount into an environment or
  • the present invention can be therefore subjected

Landscapes

  • Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Plasma & Fusion (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

Dispositif (100) d’automatisation de l’échantillonnage et de l’analyse bioluminométrique d’un fluide, comprenant une console (1) portant ses propres instruments, un luminomètre (2), un dispositif de manipulation à coulissement horizontal auquel est associée une tête à coulissement vertical (200), ladite tête à coulissement vertical (200) recevant une seringue de dosage, des moyens de stérilisation et de nettoyage de ladite seringue de dosage (19), des moyens de stockage, d’extraction et de positionnement aptes à recevoir les échantillons à analyser, un cylindre multifonction (4) doté d’un passage de remise en circulation et d’un puits d’extraction pour les échantillons de fluide et d’un certain nombre de puits d’extraction de réactif, et des moyens électroniques de traitement des données issues dudit luminomètre.
PCT/IB2006/001644 2005-12-16 2006-06-07 Dispositif d’echantillonnage et d’analyse bioluminometrique automatiques WO2007069005A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP06765552A EP1960755A1 (fr) 2005-12-16 2006-06-07 Dispositif d echantillonnage et d analyse bioluminometrique automatiques

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
ITMI2005A002407 2005-12-16
ITMI20052407 ITMI20052407A1 (it) 2005-12-16 2005-12-16 Dispositivo automatico di campionamento e analisi bioluminometrica

Publications (1)

Publication Number Publication Date
WO2007069005A1 true WO2007069005A1 (fr) 2007-06-21

Family

ID=36930334

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IB2006/001644 WO2007069005A1 (fr) 2005-12-16 2006-06-07 Dispositif d’echantillonnage et d’analyse bioluminometrique automatiques

Country Status (3)

Country Link
EP (1) EP1960755A1 (fr)
IT (1) ITMI20052407A1 (fr)
WO (1) WO2007069005A1 (fr)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2050471A1 (fr) * 2007-10-17 2009-04-22 Millipore Corporation Procédé de décontamination et système le mettant en ýuvre
WO2009129646A1 (fr) * 2008-04-21 2009-10-29 Honeywell International Inc. Micro-dispositif à base de luciférine-luciférase pour la biodétection
CN101782427A (zh) * 2010-03-12 2010-07-21 上海电机学院 照度计自动定位系统
EP2530456A1 (fr) * 2010-01-26 2012-12-05 Hitachi Plant Technologies, Ltd. Procédé et mécanisme de lavage de canalisation de dispositif de mesure d'émission de lumière

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3520660A (en) * 1966-09-09 1970-07-14 Nasa Light detection instrument
US4140487A (en) * 1975-12-10 1979-02-20 Commonwealth Scientific And Industiral Research Organization Method and apparatus for analysis of water
EP0314448A2 (fr) * 1987-10-29 1989-05-03 Cardiff Laboratories for Energy & Resources Limited Dispositif d'injection pour l'analyse d'échantillons dans des luminomètres
US4900512A (en) * 1986-12-24 1990-02-13 Nivarox-Far S.A. Apparatus for photometrically analysing liquid samples

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3520660A (en) * 1966-09-09 1970-07-14 Nasa Light detection instrument
US4140487A (en) * 1975-12-10 1979-02-20 Commonwealth Scientific And Industiral Research Organization Method and apparatus for analysis of water
US4900512A (en) * 1986-12-24 1990-02-13 Nivarox-Far S.A. Apparatus for photometrically analysing liquid samples
EP0314448A2 (fr) * 1987-10-29 1989-05-03 Cardiff Laboratories for Energy & Resources Limited Dispositif d'injection pour l'analyse d'échantillons dans des luminomètres

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2050471A1 (fr) * 2007-10-17 2009-04-22 Millipore Corporation Procédé de décontamination et système le mettant en ýuvre
FR2922453A1 (fr) * 2007-10-17 2009-04-24 Millipore Corp Procede de decontamination et systeme le mettant en oeuvre
US8071022B2 (en) 2007-10-17 2011-12-06 Millipore Corporation Decontamination method and system implementing it
US8815173B2 (en) 2007-10-17 2014-08-26 Emd Millipore Corporation Decontamination method and system implementing it
WO2009129646A1 (fr) * 2008-04-21 2009-10-29 Honeywell International Inc. Micro-dispositif à base de luciférine-luciférase pour la biodétection
CN102066911A (zh) * 2008-04-21 2011-05-18 霍尼韦尔国际公司 用于生物传感的基于萤光素-荧光素酶的微装置
EP2530456A1 (fr) * 2010-01-26 2012-12-05 Hitachi Plant Technologies, Ltd. Procédé et mécanisme de lavage de canalisation de dispositif de mesure d'émission de lumière
EP2530456A4 (fr) * 2010-01-26 2014-02-26 Hitachi Plant Technologies Ltd Procédé et mécanisme de lavage de canalisation de dispositif de mesure d'émission de lumière
CN101782427A (zh) * 2010-03-12 2010-07-21 上海电机学院 照度计自动定位系统

Also Published As

Publication number Publication date
EP1960755A1 (fr) 2008-08-27
ITMI20052407A1 (it) 2007-06-17

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