WO2007066355B1 - Processes for the preparation and estimation of enriched calcitriol containing extracts from cestrum diurnum and compositions thereof - Google Patents

Processes for the preparation and estimation of enriched calcitriol containing extracts from cestrum diurnum and compositions thereof

Info

Publication number
WO2007066355B1
WO2007066355B1 PCT/IN2006/000397 IN2006000397W WO2007066355B1 WO 2007066355 B1 WO2007066355 B1 WO 2007066355B1 IN 2006000397 W IN2006000397 W IN 2006000397W WO 2007066355 B1 WO2007066355 B1 WO 2007066355B1
Authority
WO
WIPO (PCT)
Prior art keywords
organic solvent
calcitriol
solution
bird
enriched
Prior art date
Application number
PCT/IN2006/000397
Other languages
French (fr)
Other versions
WO2007066355A1 (en
Inventor
Puchalapalli Mitra
Original Assignee
Puchalapalli Mitra
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Puchalapalli Mitra filed Critical Puchalapalli Mitra
Publication of WO2007066355A1 publication Critical patent/WO2007066355A1/en
Publication of WO2007066355B1 publication Critical patent/WO2007066355B1/en

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/81—Solanaceae (Potato family), e.g. tobacco, nightshade, tomato, belladonna, capsicum or jimsonweed
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/59—Compounds containing 9, 10- seco- cyclopenta[a]hydrophenanthrene ring systems
    • A61K31/593—9,10-Secocholestane derivatives, e.g. cholecalciferol, i.e. vitamin D3
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/12—Drugs for disorders of the metabolism for electrolyte homeostasis
    • A61P3/14—Drugs for disorders of the metabolism for electrolyte homeostasis for calcium homeostasis

Abstract

Processes for preparation of enriched extracts containing calcitriol from the leaves and inflorescence of the Day jasmine plant i.e. Cestrum Diurnam using commonly available solvents is described. The invention further discloses quantifying the calcitriol content in the extract using simple and accurate laboratory technique such as high performance liquid chromatography (HPLC). Use of the said extract in pharmaceutical and veterinary compositions are also described.

Claims

AMENDED CLAIMS received by the International Bureau on 25 May 2007 (25.05.07)
1. A process for preparation of calcitriol enriched extracts from the leaves of cestrum Diurnum comprising the steps of: a) stirring the powder of dried leaves repeatedly using a suitable first organic solvent followed by filtering the first organic solution; b) concentrating the above collected solution by distilling the first solvent to reduce the volume; c) decolorizing the above solution using activated carbon, followed by filtering the solution and ; d) evaporating the first organic solvent under vacuum to obtain enriched extract of calcitriol.
2. The process as claimed in claim 1, wherein the moisture content of the dried leaves is in the range of 10 to 20%.
3. The process as claimed in claim 1, wherein said first organic solvent is selected from ethanol or ethyl acetate.
4. The process as claimed in claim 1, comprising an optional step of distilling the first organic solvent completely and adding the second organic solvent to prepare second organic solution.
5. The process as claimed in claim 1 and 4, further comprising an optional step of washing the second organic solution repeatedly with brine solution to remove the color before decolorizing the solution.
6. The process as claimed in claim 5 further comprising a step of evaporating the second organic solvent completely and adding the first organic solvent to dissolve the residue.
7. The process as claimed in claims 4 to 6, wherein said first organic solvent is ethanol.
8. The process as claimed in claims 4 to 6, wherein said second organic solvent is ethyl acetate.
9. The process as claimed in claims 1, further comprising an optional step of washing the first organic solution repeatedly with brine solution to remove the color before decolorizing the solution.
10. The process as claimed in claim 1 and 9 further comprising a step of distilling the first organic solvent completely and adding the second organic solvent to dissolve the residue.
11. The process as claimed in claims 9 and 10, wherein said first organic solvent is ethyl acetate.
12. The process as claimed in claims 9 to 10, wherein second organic solvent is ethanol.
13. The process as claimed in any of the preceding claims, wherein the weight ratio of the dried leaf powder and the solvent used for extraction is in the range of 1 : 5 to 1:20.
14. The process as claimed in any of the preceding claims, wherein said activated carbon is used in 0.1% to 1% w/w with reference to the weight of the solution containing enriched calcitriol extract.
15. Calcitriol enriched extracts prepared from the leaves of Cestrum Diurnum according to claims 1 to 14.
16. Calcitriol content in the enriched extract as claimed in claim 15 is quantified using high performance liquid chromatography (HPLC).
17. A topical composition comprising from about 1 to 5μg of calcitriol obtained from the enriched extract of cestrum diurnum in a pharmaceutically acceptable base along with other suitable excipients for use in the treatment of psoriasis.
18. The topical composition as claimed in claim 17, wherein said base is selected from aloe vera or petroleum jelly or poly unsaturated oils.
19. The topical composition as claimed in claim 17 and 18 selected from the group consisting of ointment, cream or gel.
20. A soft gelatin capsule comprising from about 0.1 to 0.5μg of calcitriol obtained from the enriched extract of Cestrum Diurnum in pharmaceutically acceptable vehicle along with other suitable excipients for use in the treatment of rickets, renal osteodystrophy and osteoporosis. 21
21. The soft gelatin capsule as claimed in claim 20, wherein said vehicle is selected from poly alkylene glycol such as poly ethylene glycol or vegetable oil selected from com oil, peanut oil, safflower oil, sunflower oil and soybean oil.
22. A veterinary composition comprising 4 gm dry powder of the leaves of Cestrum Diurnum in one kilo of regular poultry feed for use in poultry to increase the bird weight, to increase the resistance to infection, and to decrease mortality.
23. A method of treating a skin disorder comprising plaque psoriasis in a subject, which method includes applying a topical composition according to claims 17 to 19 to the affected area of said subject.
24. A method of treatment as claimed in claim 23, wherein said subject is a mammal.
25. A method for increasing bird weight in poultry comprising administering an effective amount of veterinary composition according to claim 22 to the said bird.
26. A method for increasing the resistance in a bird comprising administering an effective amount of veterinary composition according to claim 22 to the said bird.
27. A method to decreasing the mortality in a bird comprising administering an effective amount of veterinary composition according to claim 22 to the said bird.
PCT/IN2006/000397 2005-12-06 2006-10-04 Processes for the preparation and estimation of enriched calcitriol containing extracts from cestrum diurnum and compositions thereof WO2007066355A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IN1784/CHE/2005 2005-12-06
IN1784CH2005 2005-12-06

Publications (2)

Publication Number Publication Date
WO2007066355A1 WO2007066355A1 (en) 2007-06-14
WO2007066355B1 true WO2007066355B1 (en) 2007-07-26

Family

ID=37806228

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IN2006/000397 WO2007066355A1 (en) 2005-12-06 2006-10-04 Processes for the preparation and estimation of enriched calcitriol containing extracts from cestrum diurnum and compositions thereof

Country Status (1)

Country Link
WO (1) WO2007066355A1 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AT518206A2 (en) * 2016-01-29 2017-08-15 Neovia Animal kinase supplement containing protein kinase C inhibitor
WO2017129731A1 (en) * 2016-01-29 2017-08-03 Neovia Animal feed additive containing protein kinase c inhibitor
DE102016105369A1 (en) * 2016-03-22 2017-09-28 Bernd Büter Product, in particular food additive, feed or animal feed additive and process for its production

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5776461A (en) * 1996-07-26 1998-07-07 Chesebrough-Pond's Usa Co., Division Of Conopco, Inc. Cosmetic compositions containing phytovitamin D
FR2865400B1 (en) * 2004-01-26 2008-12-26 Greentech Sa NOVEL COMPOSITION BASED ON SCROFUL EXTRACT AND BIRCH EXTRACT AND THEIR USE, IN PARTICULAR IN THE PHARMACEUTICAL OR COSMETIC FIELDS

Also Published As

Publication number Publication date
WO2007066355A1 (en) 2007-06-14

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