WO2007062063A3 - Recombinant viral compositions and uses thereof - Google Patents

Recombinant viral compositions and uses thereof Download PDF

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Publication number
WO2007062063A3
WO2007062063A3 PCT/US2006/045104 US2006045104W WO2007062063A3 WO 2007062063 A3 WO2007062063 A3 WO 2007062063A3 US 2006045104 W US2006045104 W US 2006045104W WO 2007062063 A3 WO2007062063 A3 WO 2007062063A3
Authority
WO
WIPO (PCT)
Prior art keywords
vsv
host cell
protein
another virus
genes
Prior art date
Application number
PCT/US2006/045104
Other languages
French (fr)
Other versions
WO2007062063A2 (en
Inventor
Sean Whelan
David K Cureton
Original Assignee
Harvard College
Sean Whelan
David K Cureton
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Harvard College, Sean Whelan, David K Cureton filed Critical Harvard College
Publication of WO2007062063A2 publication Critical patent/WO2007062063A2/en
Publication of WO2007062063A3 publication Critical patent/WO2007062063A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/60—Fusion polypeptide containing spectroscopic/fluorescent detection, e.g. green fluorescent protein [GFP]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
    • C12N2760/00011—Details
    • C12N2760/20011—Rhabdoviridae
    • C12N2760/20211—Vesiculovirus, e.g. vesicular stomatitis Indiana virus
    • C12N2760/20222—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/005—Assays involving biological materials from specific organisms or of a specific nature from viruses
    • G01N2333/08—RNA viruses
    • G01N2333/145—Rhabdoviridae, e.g. rabies virus, Duvenhage virus, Mokola virus or vesicular stomatitis virus

Abstract

The invention is directed to a modified vesicular stomatitis virus (VSV). the VSV is characterized by a fusion protein comprising a reporter molecule fused to a viral protein. The invention further includes genetically engineered or DNA constructs encoding such a fusion protein, either alone or in combination with one or more VSV genes and genes that encode proteins involved in attachment of VSV or another virus, bacteria or protein to a host cell, internalization of VSV or another virus into a host cell or release of VSV or another virus into the cytoplasm of a host cell. The VSVs of the invention can be used in methods for rapidly detecting viral attachment and entry and can be used, for example, in methods of rapidly identifying new antiviral therapeutics and monitoring the presence of antibodies.
PCT/US2006/045104 2005-11-23 2006-11-22 Recombinant viral compositions and uses thereof WO2007062063A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US73971905P 2005-11-23 2005-11-23
US60/739,719 2005-11-23

Publications (2)

Publication Number Publication Date
WO2007062063A2 WO2007062063A2 (en) 2007-05-31
WO2007062063A3 true WO2007062063A3 (en) 2007-10-25

Family

ID=38067871

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2006/045104 WO2007062063A2 (en) 2005-11-23 2006-11-22 Recombinant viral compositions and uses thereof

Country Status (1)

Country Link
WO (1) WO2007062063A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0809898D0 (en) 2008-06-02 2008-07-09 Univ Ghent Methods and compositions in the treatment of coronaviruses
WO2018161046A1 (en) * 2017-03-03 2018-09-07 The Johns Hopkins University Magnetic resonance method for detecting molecular binding with enhanced sensitivity
US20230346919A1 (en) * 2020-04-14 2023-11-02 Washington University Sars cov-2 vaccines and high throughput screening assays based on vesicular stomatitis virus vectors

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
BLUM ET AL.: "Development and Characterization of Enhanced Green Fluorescent Protein and Luciferase Expressing Cell Line for Non-Destructive Evaluation of Tissue Engineering Constructs", BIOMATERIALS, vol. 25, 2004, pages 5809 - 5819 *
KOLOKOLTSOV ET AL.: "Rapid and Sensitive Selection of Retrovirus Entry by Using a Novel Luciferase-Based Content-Mixing Assay", JOURNAL OF VIROLOGY, vol. 78, no. 10, May 2004 (2004-05-01), pages 5124 - 5132 *
SCHLEHUBER ET AL.: "Prediction and Identification of a Permissive Epitope Insertion Site in the Vesicular Stomatitis Virus Glycoprotein", JOURNAL OF VIROLOGY, vol. 78, no. 10, May 2004 (2004-05-01), pages 5079 - 5087 *
STILLMAN ET AL.: "Replication and Amplification of Novel Vesicular Stomatitis Virus Minigenomes Encoding Viral Structural Proteins", JOURNAL OF VIROLOGY, vol. 69, no. 5, May 1995 (1995-05-01), pages 2946 - 2953 *

Also Published As

Publication number Publication date
WO2007062063A2 (en) 2007-05-31

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