WO2007060707A3 - Reactifs et methode d'amplification et de detection simultanees d'acide nucleique - Google Patents

Reactifs et methode d'amplification et de detection simultanees d'acide nucleique Download PDF

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Publication number
WO2007060707A3
WO2007060707A3 PCT/IT2006/000815 IT2006000815W WO2007060707A3 WO 2007060707 A3 WO2007060707 A3 WO 2007060707A3 IT 2006000815 W IT2006000815 W IT 2006000815W WO 2007060707 A3 WO2007060707 A3 WO 2007060707A3
Authority
WO
WIPO (PCT)
Prior art keywords
reagents
detection
nucleic acid
acid amplification
simultaneous nucleic
Prior art date
Application number
PCT/IT2006/000815
Other languages
English (en)
Other versions
WO2007060707A2 (fr
Inventor
Daniel Adlerstein
Erlet Shehi
Nicola Alessandro Di
Original Assignee
Diasorin Spa
Daniel Adlerstein
Erlet Shehi
Nicola Alessandro Di
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from ITRM20060056 external-priority patent/ITRM20060056A1/it
Application filed by Diasorin Spa, Daniel Adlerstein, Erlet Shehi, Nicola Alessandro Di filed Critical Diasorin Spa
Priority to EP06832332A priority Critical patent/EP1969140A2/fr
Publication of WO2007060707A2 publication Critical patent/WO2007060707A2/fr
Publication of WO2007060707A3 publication Critical patent/WO2007060707A3/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates

Abstract

La présente invention concerne une méthode d'amplification et de détection simultanées d'acide nucléique, dans un environnement homogène, ainsi que des réactifs pour la mise en oeuvre de ladite méthode. Ladite méthode est avantageusement utilisée afin de détecter même de très faibles quantités de l'acide nucléique cible.
PCT/IT2006/000815 2005-11-23 2006-11-22 Reactifs et methode d'amplification et de detection simultanees d'acide nucleique WO2007060707A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP06832332A EP1969140A2 (fr) 2005-11-23 2006-11-22 Réactifs et méthode d'amplification et de détection simultanées d'acide nucléique

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US73929605P 2005-11-23 2005-11-23
US60/739,296 2005-11-23
ITRM2006A000056 2006-02-03
ITRM20060056 ITRM20060056A1 (it) 2006-02-03 2006-02-03 Reagenti e metodo per amplificazione e rilevazione simultanea di acidi nucleici

Publications (2)

Publication Number Publication Date
WO2007060707A2 WO2007060707A2 (fr) 2007-05-31
WO2007060707A3 true WO2007060707A3 (fr) 2007-10-18

Family

ID=38067643

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IT2006/000815 WO2007060707A2 (fr) 2005-11-23 2006-11-22 Reactifs et methode d'amplification et de detection simultanees d'acide nucleique

Country Status (2)

Country Link
EP (1) EP1969140A2 (fr)
WO (1) WO2007060707A2 (fr)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101809170B (zh) * 2007-09-28 2014-08-20 3M创新有限公司 用于检测核酸的双寡核苷酸方法
US9404150B2 (en) 2007-08-29 2016-08-02 Sequenom, Inc. Methods and compositions for universal size-specific PCR

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1905842A1 (fr) 2006-09-26 2008-04-02 DiaSorin S.p.A. Procédé de détection d'alleles mutants combinant real time pcr et rems-pcr
US7902345B2 (en) 2006-12-05 2011-03-08 Sequenom, Inc. Detection and quantification of biomolecules using mass spectrometry
WO2010107946A2 (fr) * 2009-03-18 2010-09-23 Sequenom, Inc. Utilisation d'endonucléases thermostables pour générer des molécules rapporteuses
US8916349B2 (en) 2009-11-23 2014-12-23 Becton, Dickinson And Company Assay method for target nucleic acid by signal amplification using probe hybridization and restriction
CN110552695A (zh) * 2019-09-26 2019-12-10 中国地质大学（北京） 一种不同油价下海上油藏聚合物驱可行性的快速评价方法

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995014770A1 (fr) * 1993-11-25 1995-06-01 Pacific Enzymes (1993) Limited Polymerase amelioree
US5627032A (en) * 1990-12-24 1997-05-06 Ulanovsky; Levy Composite primers for nucleic acids
WO1999028501A1 (fr) * 1997-11-29 1999-06-10 The Secretary Of State For Defence Systeme fluorometrique d'observation et de detection de l'amplification d'acides nucleiques
US5958700A (en) * 1997-05-30 1999-09-28 Becton, Dickinson And Company Detection of nucleic acids by fluorescence quenching
US6235889B1 (en) * 1991-12-20 2001-05-22 University Of Chicago Nucleic acid amplification using modular branched primers

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5627032A (en) * 1990-12-24 1997-05-06 Ulanovsky; Levy Composite primers for nucleic acids
US6235889B1 (en) * 1991-12-20 2001-05-22 University Of Chicago Nucleic acid amplification using modular branched primers
WO1995014770A1 (fr) * 1993-11-25 1995-06-01 Pacific Enzymes (1993) Limited Polymerase amelioree
US5958700A (en) * 1997-05-30 1999-09-28 Becton, Dickinson And Company Detection of nucleic acids by fluorescence quenching
WO1999028501A1 (fr) * 1997-11-29 1999-06-10 The Secretary Of State For Defence Systeme fluorometrique d'observation et de detection de l'amplification d'acides nucleiques

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
CAIRNS M J ET AL: "Homogeneous real-time detection and quantification of nucleic acid amplification using restriction enzyme digestion", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, ACADEMIC PRESS INC. ORLANDO, FL, US, vol. 318, no. 3, 4 June 2004 (2004-06-04), pages 684 - 690, XP004508488, ISSN: 0006-291X *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9404150B2 (en) 2007-08-29 2016-08-02 Sequenom, Inc. Methods and compositions for universal size-specific PCR
CN101809170B (zh) * 2007-09-28 2014-08-20 3M创新有限公司 用于检测核酸的双寡核苷酸方法

Also Published As

Publication number Publication date
WO2007060707A2 (fr) 2007-05-31
EP1969140A2 (fr) 2008-09-17

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