WO2007057668A1 - Methods using pores - Google Patents
Methods using pores Download PDFInfo
- Publication number
- WO2007057668A1 WO2007057668A1 PCT/GB2006/004265 GB2006004265W WO2007057668A1 WO 2007057668 A1 WO2007057668 A1 WO 2007057668A1 GB 2006004265 W GB2006004265 W GB 2006004265W WO 2007057668 A1 WO2007057668 A1 WO 2007057668A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- pore
- nucleotide
- sequence
- nucleic acid
- current
- Prior art date
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/483—Physical analysis of biological material
- G01N33/487—Physical analysis of biological material of liquid biological material
- G01N33/48707—Physical analysis of biological material of liquid biological material by electrical means
- G01N33/48721—Investigating individual macromolecules, e.g. by translocation through nanopores
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/483—Physical analysis of biological material
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
- C12Q2565/60—Detection means characterised by use of a special device
- C12Q2565/631—Detection means characterised by use of a special device being a biochannel or pore
Abstract
Description
Claims
Priority Applications (11)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
NZ568163A NZ568163A (en) | 2005-11-15 | 2006-11-15 | Methods of identifying individual nucleotides using pores |
US12/093,610 US8105846B2 (en) | 2005-11-15 | 2006-11-15 | Methods using pores |
CN200680050878.XA CN101356288B (en) | 2005-11-15 | 2006-11-15 | Use the method in hole |
EP06808555A EP1951898B1 (en) | 2005-11-15 | 2006-11-15 | Methods using pores |
AU2006314296A AU2006314296B2 (en) | 2005-11-15 | 2006-11-15 | Methods using pores |
BRPI0618391-3A BRPI0618391A2 (en) | 2005-11-15 | 2006-11-15 | method for identifying an individual nucleotide, method for sequencing a target nucleic acid sequence, and kit for sequencing a nucleic acid |
JP2008540686A JP5328362B2 (en) | 2005-11-15 | 2006-11-15 | How to use pores |
AT06808555T ATE545712T1 (en) | 2005-11-15 | 2006-11-15 | METHOD USING PORES |
CA2629009A CA2629009C (en) | 2005-11-15 | 2006-11-15 | Methods using pores |
IL191319A IL191319A (en) | 2005-11-15 | 2008-05-07 | Method of identifying an individual nucleotide using a transmembrane protein pore |
US13/338,794 US8785211B2 (en) | 2005-11-15 | 2011-12-28 | Methods using pores |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GBGB0523282.2A GB0523282D0 (en) | 2005-11-15 | 2005-11-15 | Methods using pores |
GB0523282.2 | 2005-11-15 |
Related Child Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US12/093,610 A-371-Of-International US8105846B2 (en) | 2005-11-15 | 2006-11-15 | Methods using pores |
US13/338,794 Continuation US8785211B2 (en) | 2005-11-15 | 2011-12-28 | Methods using pores |
Publications (1)
Publication Number | Publication Date |
---|---|
WO2007057668A1 true WO2007057668A1 (en) | 2007-05-24 |
Family
ID=35516986
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/GB2006/004265 WO2007057668A1 (en) | 2005-11-15 | 2006-11-15 | Methods using pores |
Country Status (16)
Country | Link |
---|---|
US (2) | US8105846B2 (en) |
EP (2) | EP2272981B1 (en) |
JP (1) | JP5328362B2 (en) |
KR (1) | KR20080083281A (en) |
CN (1) | CN101356288B (en) |
AT (1) | ATE545712T1 (en) |
AU (1) | AU2006314296B2 (en) |
BR (1) | BRPI0618391A2 (en) |
CA (1) | CA2629009C (en) |
GB (1) | GB0523282D0 (en) |
IL (1) | IL191319A (en) |
MY (1) | MY149161A (en) |
NZ (1) | NZ568163A (en) |
RU (1) | RU2008123842A (en) |
UA (1) | UA95087C2 (en) |
WO (1) | WO2007057668A1 (en) |
Cited By (54)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB2453377A (en) * | 2007-10-05 | 2009-04-08 | Isis Innovation | Transmembrane protein pores and molecular adapters therefore. |
WO2010004265A1 (en) * | 2008-07-07 | 2010-01-14 | Oxford Nanopore Technologies Limited | Enzyme-pore constructs |
WO2010004273A1 (en) * | 2008-07-07 | 2010-01-14 | Oxford Nanopore Technologies Limited | Base-detecting pore |
WO2010055307A1 (en) * | 2008-11-14 | 2010-05-20 | Isis Innovation Limited | Methods of enhancing translocation of charged analytes through transmembrane protein pores |
WO2010086603A1 (en) * | 2009-01-30 | 2010-08-05 | Oxford Nanopore Technologies Limited | Enzyme mutant |
WO2010109197A3 (en) * | 2009-03-25 | 2011-03-17 | Isis Innovation Limited | Method for sequencing a heteropolymeric target nucleic acid sequence |
WO2011067559A1 (en) | 2009-12-01 | 2011-06-09 | Oxford Nanopore Technologies Limited | Biochemical analysis instrument |
WO2012042226A2 (en) | 2010-10-01 | 2012-04-05 | Oxford Nanopore Technologies Limited | Biochemical analysis apparatus and rotary valve |
JP2012516146A (en) * | 2009-01-30 | 2012-07-19 | オックスフォード ナノポア テクノロジーズ リミテッド | Adapters for nucleic acid constructs in transmembrane sequencing |
WO2013057495A3 (en) * | 2011-10-21 | 2013-08-22 | Oxford Nanopore Technologies Limited | Method of characterizing a target polynucleotide using a pore and a hel308 helicase |
WO2014013259A1 (en) * | 2012-07-19 | 2014-01-23 | Oxford Nanopore Technologies Limited | Ssb method |
WO2014013262A1 (en) * | 2012-07-19 | 2014-01-23 | Oxford Nanopore Technologies Limited | Enzyme construct |
US8785211B2 (en) | 2005-11-15 | 2014-07-22 | Isis Innovation Limited | Methods using pores |
EP2851433A1 (en) * | 2008-08-01 | 2015-03-25 | Brown University | System and methods for determining molecules using mass spectrometry and related techniques |
US9557294B2 (en) | 2014-12-19 | 2017-01-31 | Genia Technologies, Inc. | Nanopore-based sequencing with varying voltage stimulus |
US9617591B2 (en) | 2011-12-29 | 2017-04-11 | Oxford Nanopore Technologies Ltd. | Method for characterising a polynucleotide by using a XPD helicase |
US9751915B2 (en) | 2011-02-11 | 2017-09-05 | Oxford Nanopore Technologies Ltd. | Mutant pores |
US9777049B2 (en) | 2012-04-10 | 2017-10-03 | Oxford Nanopore Technologies Ltd. | Mutant lysenin pores |
US9863904B2 (en) | 2014-12-19 | 2018-01-09 | Genia Technologies, Inc. | Nanopore-based sequencing with varying voltage stimulus |
US9957560B2 (en) | 2011-07-25 | 2018-05-01 | Oxford Nanopore Technologies Ltd. | Hairpin loop method for double strand polynucleotide sequencing using transmembrane pores |
US10006905B2 (en) | 2013-03-25 | 2018-06-26 | Katholieke Universiteit Leuven | Nanopore biosensors for detection of proteins and nucleic acids |
US10126262B2 (en) | 2015-09-24 | 2018-11-13 | Genia Technologies, Inc. | Differential output of analog memories storing nanopore measurement samples |
US10167503B2 (en) | 2014-05-02 | 2019-01-01 | Oxford Nanopore Technologies Ltd. | Mutant pores |
US10221450B2 (en) | 2013-03-08 | 2019-03-05 | Oxford Nanopore Technologies Ltd. | Enzyme stalling method |
US10266885B2 (en) | 2014-10-07 | 2019-04-23 | Oxford Nanopore Technologies Ltd. | Mutant pores |
US10337060B2 (en) | 2014-04-04 | 2019-07-02 | Oxford Nanopore Technologies Ltd. | Method for characterising a double stranded nucleic acid using a nano-pore and anchor molecules at both ends of said nucleic acid |
US10385382B2 (en) | 2011-12-29 | 2019-08-20 | Oxford Nanopore Technologies Ltd. | Enzyme method |
US10385389B2 (en) | 2014-01-22 | 2019-08-20 | Oxford Nanopore Technologies Ltd. | Method for attaching one or more polynucleotide binding proteins to a target polynucleotide |
US10392658B2 (en) | 2014-01-22 | 2019-08-27 | Oxford Nanopore Technologies Ltd. | Method for controlling the movement of a polynucleotide through a transmembrane pore |
US10400014B2 (en) | 2014-09-01 | 2019-09-03 | Oxford Nanopore Technologies Ltd. | Mutant CsgG pores |
US10472673B2 (en) | 2015-02-19 | 2019-11-12 | Oxford Nanopore Technologies Ltd. | Hetero-pores |
US10501767B2 (en) | 2013-08-16 | 2019-12-10 | Oxford Nanopore Technologies Ltd. | Polynucleotide modification methods |
US10570440B2 (en) | 2014-10-14 | 2020-02-25 | Oxford Nanopore Technologies Ltd. | Method for modifying a template double stranded polynucleotide using a MuA transposase |
US10669578B2 (en) | 2014-02-21 | 2020-06-02 | Oxford Nanopore Technologies Ltd. | Sample preparation method |
US20200171487A1 (en) * | 2017-04-19 | 2020-06-04 | Electronic Biosciences, Inc. | Nanopore/nanowell electrode enabled exonuclease sequencing |
US10724018B2 (en) | 2013-10-18 | 2020-07-28 | Oxford Nanopore Technologies Ltd. | Modified helicases |
US10760114B2 (en) | 2014-10-17 | 2020-09-01 | Oxford Nanopore Technologies Ltd. | Methods for delivering an analyte to transmembrane pores |
US10774378B2 (en) | 2014-04-04 | 2020-09-15 | Oxford Nanopore Technologies Ltd. | Method of target molecule characterisation using a molecular pore |
US10808231B2 (en) | 2012-07-19 | 2020-10-20 | Oxford Nanopore Technologies Limited | Modified helicases |
US10948455B2 (en) | 2014-06-25 | 2021-03-16 | Huldagate Technologies Limited | Sequencer |
US10975428B2 (en) | 2016-03-02 | 2021-04-13 | Oxford Nanopore Technologies Ltd. | Mutant pore |
US10976300B2 (en) | 2015-12-08 | 2021-04-13 | Katholieke Universiteit Leuven | Modified nanopores, compositions comprising the same, and uses thereof |
US11041194B2 (en) | 2011-05-27 | 2021-06-22 | Oxford Nanopore Technologies Ltd. | Coupling method |
US11104709B2 (en) | 2016-04-06 | 2021-08-31 | Oxford Nanopore Technologies Ltd. | Mutant pore |
US11169138B2 (en) | 2015-04-14 | 2021-11-09 | Katholieke Universiteit Leuven | Nanopores with internal protein adaptors |
US11180741B2 (en) | 2014-10-07 | 2021-11-23 | Oxford Nanopore Technologies Ltd. | Modified enzymes |
US11261488B2 (en) | 2015-09-24 | 2022-03-01 | Roche Sequencing Solutions, Inc. | Alpha-hemolysin variants |
US11307192B2 (en) | 2015-02-19 | 2022-04-19 | Oxford Nanopore Technologies Plc | Method for producing a hetero-oligomeric pore comprising two different monomers in a specific stiochiometric ratio |
US11479584B2 (en) | 2014-10-31 | 2022-10-25 | Roche Sequencing Solutions, Inc. | Alpha-hemolysin variants with altered characteristics |
US11572387B2 (en) | 2017-06-30 | 2023-02-07 | Vib Vzw | Protein pores |
US11639524B2 (en) | 2017-11-27 | 2023-05-02 | Roche Sequencing Solutions, Inc. | Normalization and baseline shift removal by rotation in added data dimensions |
US11649480B2 (en) | 2016-05-25 | 2023-05-16 | Oxford Nanopore Technologies Plc | Method for modifying a template double stranded polynucleotide |
US11725205B2 (en) | 2018-05-14 | 2023-08-15 | Oxford Nanopore Technologies Plc | Methods and polynucleotides for amplifying a target polynucleotide |
WO2023161625A1 (en) | 2022-02-28 | 2023-08-31 | Oxford Nanopore Technologies Plc | Apparatus and methods for controlling insertion of a membrane channel into a membrane |
Families Citing this family (32)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP3543357A1 (en) | 2007-05-08 | 2019-09-25 | Trustees of Boston University | Chemical functionalization of solid-state nanopores and nanopore arrays and applications thereof |
US20100148126A1 (en) * | 2008-11-26 | 2010-06-17 | Board Of Regents, The University Of Texas System | Genomic sequencing using modified protein pores and ionic liquids |
WO2010117470A2 (en) | 2009-04-10 | 2010-10-14 | Pacific Biosciences Of California, Inc. | Nanopore sequencing devices and methods |
US9017937B1 (en) | 2009-04-10 | 2015-04-28 | Pacific Biosciences Of California, Inc. | Nanopore sequencing using ratiometric impedance |
EP2483680A4 (en) | 2009-09-30 | 2014-01-01 | Quantapore Inc | Ultrafast sequencing of biological polymers using a labeled nanopore |
US9005425B2 (en) | 2010-03-05 | 2015-04-14 | University Of Utah Research Foundation | Detection of nucleic acid lesions and adducts using nanopores |
GB2490846A (en) * | 2010-03-05 | 2012-11-14 | Univ Utah Res Found | Detection of nucleic acid lesions and adducts using nanopores |
US8652779B2 (en) | 2010-04-09 | 2014-02-18 | Pacific Biosciences Of California, Inc. | Nanopore sequencing using charge blockade labels |
US8673757B2 (en) | 2010-10-28 | 2014-03-18 | International Business Machines Corporation | Structure and method for using high-k material as an etch stop layer in dual stress layer process |
RU2467218C1 (en) * | 2011-06-16 | 2012-11-20 | Закрытое Акционерное Общество "Научно-производственное объединение "Гидравлические аппараты" (ЗАО "НПО "Гидроаппарат") | Hydraulic coupling |
ES2659343T3 (en) * | 2011-07-20 | 2018-03-14 | The Regents Of The University Of California | Dual pore device |
AU2013220156B2 (en) | 2012-02-15 | 2018-08-09 | Oxford Nanopore Technologies Limited | Aptamer method |
CN109061128A (en) * | 2012-04-02 | 2018-12-21 | 力士生物集团公司 | Device and method for molecule separation, purifying and detection |
US9551023B2 (en) | 2012-09-14 | 2017-01-24 | Oxford Nanopore Technologies Ltd. | Sample preparation method |
US9651539B2 (en) | 2012-10-28 | 2017-05-16 | Quantapore, Inc. | Reducing background fluorescence in MEMS materials by low energy ion beam treatment |
WO2014072703A1 (en) | 2012-11-06 | 2014-05-15 | Oxford Nanopore Technologies Limited | Quadruplex method |
GB201318465D0 (en) | 2013-10-18 | 2013-12-04 | Oxford Nanopore Tech Ltd | Method |
CN103267785B (en) * | 2013-05-14 | 2016-04-13 | 西北大学 | A kind of single molecule analysis method of detection of drugs parcel or dispose procedure |
CN105283560B (en) | 2013-05-24 | 2018-11-30 | 昆塔波尔公司 | The foranalysis of nucleic acids detected by mixed FRET based on nano-pore |
EP3004378B1 (en) * | 2013-05-24 | 2017-12-20 | Illumina Cambridge Limited | Pyrophosphorolytic sequencing using nanopores |
GB201316849D0 (en) | 2013-09-23 | 2013-11-06 | Isis Innovation | Method |
CN107109472B (en) | 2014-10-10 | 2021-05-11 | 昆塔波尔公司 | Nanopore-based polymer analysis using mutually quenching fluorescent labels |
KR102457147B1 (en) | 2014-10-17 | 2022-10-19 | 옥스포드 나노포어 테크놀로지즈 피엘씨 | Method for nanopore rna characterisation |
JP6757316B2 (en) | 2014-10-24 | 2020-09-16 | クアンタポール, インコーポレイテッド | Efficient optical analysis of polymers using nanostructured arrays |
US10060903B2 (en) * | 2014-11-05 | 2018-08-28 | Genia Technologies, Inc. | Exporting measurements of nanopore arrays |
EP3254109A4 (en) * | 2015-02-02 | 2018-07-04 | Two Pore Guys, Inc. | Labile linkers for biomarker detection |
CN105259229B (en) * | 2015-10-22 | 2018-04-20 | 清华大学 | A kind of single molecule analysis method for detecting medicine |
US10280411B2 (en) | 2015-10-27 | 2019-05-07 | Pacific Biosciences of California, In.c | Methods, systems, and reagents for direct RNA sequencing |
JP6579948B2 (en) * | 2015-12-24 | 2019-09-25 | 株式会社日立ハイテクノロジーズ | Measuring reagent and analyzing device for analyzing biological polymer |
CN109196116B (en) * | 2016-05-25 | 2022-09-27 | 牛津纳米孔科技公开有限公司 | Method for characterizing target polynucleotide |
EP3482196B1 (en) | 2016-07-05 | 2022-02-23 | Quantapore, Inc. | Optically based nanopore sequencing |
CN110267974A (en) * | 2017-02-10 | 2019-09-20 | 牛津纳米孔技术公司 | The nano-pore of modification includes its composition and application thereof |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1999005167A1 (en) * | 1997-07-25 | 1999-02-04 | University Of Massachusetts | Designed protein pores as components for biosensors |
WO2000028312A1 (en) * | 1998-11-06 | 2000-05-18 | The Regents Of The University Of California | A miniature support for thin films containing single channels or nanopores and methods for using same |
WO2001042782A1 (en) * | 1999-12-09 | 2001-06-14 | President And Fellows Of Harvard College | Characterization of hybridized polymer molecules based on monomer-interface interactions |
Family Cites Families (38)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FI82266C (en) | 1982-10-19 | 1991-02-11 | Cetus Corp | Process for Preparation of IL-2 Mutein |
US5777078A (en) | 1993-04-28 | 1998-07-07 | Worcester Foundation For Experimental Biology | Triggered pore-forming agents |
EP0753071A1 (en) | 1993-04-28 | 1997-01-15 | Worcester Foundation For Experimental Biology | Cell-targeted lytic pore-forming agents |
US5795782A (en) | 1995-03-17 | 1998-08-18 | President & Fellows Of Harvard College | Characterization of individual polymer molecules based on monomer-interface interactions |
US6087099A (en) | 1997-09-08 | 2000-07-11 | Myriad Genetics, Inc. | Method for sequencing both strands of a double stranded DNA in a single sequencing reaction |
US6127166A (en) | 1997-11-03 | 2000-10-03 | Bayley; Hagan | Molluscan ligament polypeptides and genes encoding them |
JPH11137260A (en) | 1997-11-06 | 1999-05-25 | Soyaku Gijutsu Kenkyusho:Kk | Anti-influenza viral cyclic dumbbell type rna-dna chimera compound and anti-influenza viral agent |
US6123819A (en) | 1997-11-12 | 2000-09-26 | Protiveris, Inc. | Nanoelectrode arrays |
DE19826758C1 (en) | 1998-06-15 | 1999-10-21 | Soft Gene Gmbh | Production of closed, double-stranded DNA molecules for use in gene therapy or genetic vaccination |
US6426231B1 (en) | 1998-11-18 | 2002-07-30 | The Texas A&M University System | Analyte sensing mediated by adapter/carrier molecules |
NO986133D0 (en) | 1998-12-23 | 1998-12-23 | Preben Lexow | Method of DNA Sequencing |
US6627067B1 (en) | 1999-06-22 | 2003-09-30 | President And Fellows Of Harvard College | Molecular and atomic scale evaluation of biopolymers |
EP1196434A2 (en) | 1999-06-29 | 2002-04-17 | University Health Network | Peptide conjugates for the stabilization of membrane proteins and interactions with biological membranes |
JP2003532380A (en) | 1999-12-02 | 2003-11-05 | モレキュラー ステージング,インコーポレイテッド | Production of single-stranded circular DNA from linear self-annealing segments |
EP2083015B1 (en) | 2000-02-11 | 2016-04-06 | The Texas A & M University System | Biosensor compositions and methods of use |
US7001792B2 (en) | 2000-04-24 | 2006-02-21 | Eagle Research & Development, Llc | Ultra-fast nucleic acid sequencing device and a method for making and using the same |
US6936433B2 (en) | 2000-11-27 | 2005-08-30 | The Regents Of The University Of California | Methods and devices for characterizing duplex nucleic acid molecules |
US7807408B2 (en) | 2001-03-19 | 2010-10-05 | President & Fellows Of Harvard College | Directed evolution of proteins |
WO2002101038A1 (en) * | 2001-05-29 | 2002-12-19 | Asahi Glass Company, Limited | Method of constructing host and process for producing foreign protein |
US6863833B1 (en) | 2001-06-29 | 2005-03-08 | The Board Of Trustees Of The Leland Stanford Junior University | Microfabricated apertures for supporting bilayer lipid membranes |
EP1504114B1 (en) | 2002-05-10 | 2017-07-12 | The Texas A & M University System | Stochastic sensing through covalent interactions |
US7163658B2 (en) | 2003-04-23 | 2007-01-16 | Rouvain Bension | Rapid sequencing of polymers |
WO2005056750A2 (en) | 2003-12-11 | 2005-06-23 | Quark Biotech, Inc. | Inversion-duplication of nucleic acids and libraries prepared thereby |
US7238485B2 (en) | 2004-03-23 | 2007-07-03 | President And Fellows Of Harvard College | Methods and apparatus for characterizing polynucleotides |
CN101103357B (en) | 2004-08-13 | 2012-10-03 | 哈佛学院院长等 | An ultra high-throughput opti-nanopore DNA readout platform |
EP1842061A4 (en) | 2004-12-21 | 2009-05-13 | Texas A & M Univ Sys | High temperature ion channels and pores |
GB0505971D0 (en) | 2005-03-23 | 2005-04-27 | Isis Innovation | Delivery of molecules to a lipid bilayer |
GB0523282D0 (en) | 2005-11-15 | 2005-12-21 | Isis Innovation | Methods using pores |
ATE518010T1 (en) | 2005-12-22 | 2011-08-15 | Pacific Biosciences California | ACTIVE SURFACE COUPLED POLYMERASES |
US7932029B1 (en) | 2006-01-04 | 2011-04-26 | Si Lok | Methods for nucleic acid mapping and identification of fine-structural-variations in nucleic acids and utilities |
US20100311602A1 (en) | 2006-10-13 | 2010-12-09 | J. Craig Venter Institute, Inc. | Sequencing method |
DK2122344T3 (en) | 2007-02-20 | 2019-07-15 | Oxford Nanopore Tech Ltd | Lipid bilayer SENSOR SYSTEM |
US20110005918A1 (en) | 2007-04-04 | 2011-01-13 | Akeson Mark A | Compositions, devices, systems, and methods for using a nanopore |
GB2453377A (en) | 2007-10-05 | 2009-04-08 | Isis Innovation | Transmembrane protein pores and molecular adapters therefore. |
JP2011515102A (en) | 2008-03-28 | 2011-05-19 | パシフィック バイオサイエンシーズ オブ カリフォルニア, インコーポレイテッド | Compositions and methods for nucleic acid sequencing |
AU2009269792A1 (en) * | 2008-07-07 | 2010-01-14 | Oxford Nanopore Technologies Limited | Base-detecting pore |
CN103695530B (en) | 2008-07-07 | 2016-05-25 | 牛津纳米孔技术有限公司 | Enzyme-hole construct |
CA3092369A1 (en) | 2008-09-22 | 2010-03-25 | University Of Washington | Msp nanopores and related methods |
-
2005
- 2005-11-15 GB GBGB0523282.2A patent/GB0523282D0/en not_active Ceased
-
2006
- 2006-11-15 EP EP10010418A patent/EP2272981B1/en active Active
- 2006-11-15 KR KR1020087014358A patent/KR20080083281A/en not_active Application Discontinuation
- 2006-11-15 MY MYPI20081556A patent/MY149161A/en unknown
- 2006-11-15 WO PCT/GB2006/004265 patent/WO2007057668A1/en active Application Filing
- 2006-11-15 UA UAA200806147A patent/UA95087C2/en unknown
- 2006-11-15 AU AU2006314296A patent/AU2006314296B2/en active Active
- 2006-11-15 AT AT06808555T patent/ATE545712T1/en active
- 2006-11-15 EP EP06808555A patent/EP1951898B1/en active Active
- 2006-11-15 US US12/093,610 patent/US8105846B2/en active Active
- 2006-11-15 JP JP2008540686A patent/JP5328362B2/en active Active
- 2006-11-15 RU RU2008123842/13A patent/RU2008123842A/en not_active Application Discontinuation
- 2006-11-15 BR BRPI0618391-3A patent/BRPI0618391A2/en not_active IP Right Cessation
- 2006-11-15 NZ NZ568163A patent/NZ568163A/en not_active IP Right Cessation
- 2006-11-15 CA CA2629009A patent/CA2629009C/en active Active
- 2006-11-15 CN CN200680050878.XA patent/CN101356288B/en active Active
-
2008
- 2008-05-07 IL IL191319A patent/IL191319A/en not_active IP Right Cessation
-
2011
- 2011-12-28 US US13/338,794 patent/US8785211B2/en active Active
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1999005167A1 (en) * | 1997-07-25 | 1999-02-04 | University Of Massachusetts | Designed protein pores as components for biosensors |
WO2000028312A1 (en) * | 1998-11-06 | 2000-05-18 | The Regents Of The University Of California | A miniature support for thin films containing single channels or nanopores and methods for using same |
WO2001042782A1 (en) * | 1999-12-09 | 2001-06-14 | President And Fellows Of Harvard College | Characterization of hybridized polymer molecules based on monomer-interface interactions |
Non-Patent Citations (10)
Title |
---|
AKESON M. ET AL.: "MICROSECOND TIME-SCALE DISCRIMINATION AMONG POLYCYTIDYLIC ACID, POLYADENYLIC ACID, AND POLYURIDYLIC ACID AS HOMOPOLYMERS OR AS SEGMENTS WITHIN SINGLE RNA MOLECULES", BIOPHYSICAL JOURNAL, NEW YORK, US, US, vol. 77, no. 6, December 1999 (1999-12-01), pages 3227 - 3233, XP000914263, ISSN: 0006-3495 * |
BAYLEY H. ET AL.: "Stochastic sensors inspired by biology", NATURE, NATURE PUBLISHING GROUP, LONDON, GB, vol. 413, no. 6852, 13 September 2001 (2001-09-13), pages 226 - 230, XP002252374, ISSN: 0028-0836 * |
DAPPRICH J.: "SINGLE-MOLECULE DNA DIGESTION BY LAMBDA-EXONUCLEASE", CYTOMETRY, ALAN R. LISS, INC, XX, vol. 36, no. 3, 1 July 1999 (1999-07-01), pages 163 - 168, XP009058665, ISSN: 0196-4763 * |
GU L.-Q. ET AL.: "Stochastic sensing of organic analytes by a pore-forming protein containing a molecular adapter", NATURE, NATURE PUBLISHING GROUP, LONDON, GB, vol. 398, no. 6729, 22 April 1999 (1999-04-22), pages 686 - 690, XP002252375, ISSN: 0028-0836 * |
KASIANOWICZ J.J. ET AL.: "Characterization of individual polynucleotide molecules using a membrane channel", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, NATIONAL ACADEMY OF SCIENCE, WASHINGTON, DC, US, vol. 93, November 1996 (1996-11-01), pages 13770 - 13773, XP002139846, ISSN: 0027-8424 * |
LI J. ET AL.: "DNA MOLECULES AND CONFIGURATIONS IN A SOLID-STATE NANOPORE MICROSCOPE", NATURE, NATURE PUBLISHING GROUP, LONDON, GB, vol. 2, no. 9, September 2003 (2003-09-01), pages 611 - 615, XP009039572, ISSN: 0028-0836 * |
MARZIALI A. ET AL.: "New DNA sequencing methods.", ANNUAL REVIEW OF BIOMEDICAL ENGINEERING 2001, vol. 3, 2001, pages 195 - 223, XP009077868, ISSN: 1523-9829 * |
MATSUURA S. ET AL.: "Real-time observation of a single DNA digestion by lambda exonuclease under a fluorescence microscope field.", NUCLEIC ACIDS RESEARCH 15 AUG 2001, vol. 29, no. 16, 15 August 2001 (2001-08-15), pages E79, XP002418123, ISSN: 1362-4962 * |
MELLER A. ET AL.: "Rapid nanopore discrimination between single polynucleotide molecules", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, vol. 97, no. 3, 1 February 2000 (2000-02-01), pages 1079 - 1084, XP002418122, ISSN: 0027-8424 * |
XIE H. ET AL.: "Single-Molecule Observation of the Catalytic Subunit of cAMP-Dependent Protein Kinase Binding to an Inhibitor Peptide", CHEMISTRY AND BIOLOGY, CURRENT BIOLOGY, LONDON, GB, vol. 12, no. 1, January 2005 (2005-01-01), pages 109 - 120, XP004722469, ISSN: 1074-5521 * |
Cited By (116)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US8785211B2 (en) | 2005-11-15 | 2014-07-22 | Isis Innovation Limited | Methods using pores |
JP2010539966A (en) * | 2007-10-05 | 2010-12-24 | イシス イノベーション リミテッド | Molecular adapter |
WO2009044170A1 (en) * | 2007-10-05 | 2009-04-09 | Isis Innovation Limited | Molecular adaptors |
US8822160B2 (en) | 2007-10-05 | 2014-09-02 | Isis Innovation Limited | Molecular adaptors |
GB2453377A (en) * | 2007-10-05 | 2009-04-08 | Isis Innovation | Transmembrane protein pores and molecular adapters therefore. |
US11078530B2 (en) | 2008-07-07 | 2021-08-03 | Oxford Nanopore Technologies Ltd. | Enzyme-pore constructs |
US10077471B2 (en) | 2008-07-07 | 2018-09-18 | Oxford Nanopore Technologies Ltd. | Enzyme-pore constructs |
US9885078B2 (en) | 2008-07-07 | 2018-02-06 | Oxford Nanopore Technologies Limited | Enzyme-pore constructs |
CN103695530A (en) * | 2008-07-07 | 2014-04-02 | 牛津纳米孔技术有限公司 | Enzyme-pore constructs |
CN102144037A (en) * | 2008-07-07 | 2011-08-03 | 牛津纳米孔技术有限公司 | Base-detecting pore |
US9447152B2 (en) | 2008-07-07 | 2016-09-20 | Oxford Nanopore Technologies Limited | Base-detecting pore |
CN103695530B (en) * | 2008-07-07 | 2016-05-25 | 牛津纳米孔技术有限公司 | Enzyme-hole construct |
WO2010004273A1 (en) * | 2008-07-07 | 2010-01-14 | Oxford Nanopore Technologies Limited | Base-detecting pore |
EP2682460A1 (en) * | 2008-07-07 | 2014-01-08 | Oxford Nanopore Technologies Limited | Enzyme-pore constructs |
WO2010004265A1 (en) * | 2008-07-07 | 2010-01-14 | Oxford Nanopore Technologies Limited | Enzyme-pore constructs |
US11859247B2 (en) | 2008-07-07 | 2024-01-02 | Oxford Nanopore Technologies Plc | Enzyme-pore constructs |
EP2851433A1 (en) * | 2008-08-01 | 2015-03-25 | Brown University | System and methods for determining molecules using mass spectrometry and related techniques |
CN102317310A (en) * | 2008-11-14 | 2012-01-11 | Isis创新有限公司 | Methods of enhancing translocation of charged analytes through transmembrane protein pores |
US9562887B2 (en) | 2008-11-14 | 2017-02-07 | Oxford University Innovation Limited | Methods of enhancing translocation of charged analytes through transmembrane protein pores |
WO2010055307A1 (en) * | 2008-11-14 | 2010-05-20 | Isis Innovation Limited | Methods of enhancing translocation of charged analytes through transmembrane protein pores |
US11459606B2 (en) | 2009-01-30 | 2022-10-04 | Oxford Nanopore Technologies Plc | Adaptors for nucleic acid constructs in transmembrane sequencing |
US9222082B2 (en) | 2009-01-30 | 2015-12-29 | Oxford Nanopore Technologies Limited | Hybridization linkers |
JP2012516146A (en) * | 2009-01-30 | 2012-07-19 | オックスフォード ナノポア テクノロジーズ リミテッド | Adapters for nucleic acid constructs in transmembrane sequencing |
US11352664B2 (en) | 2009-01-30 | 2022-06-07 | Oxford Nanopore Technologies Plc | Adaptors for nucleic acid constructs in transmembrane sequencing |
WO2010086603A1 (en) * | 2009-01-30 | 2010-08-05 | Oxford Nanopore Technologies Limited | Enzyme mutant |
EP3677691A1 (en) * | 2009-03-25 | 2020-07-08 | Oxford University Innovation Limited | Method for sequencing a heteropolymeric target nucleic acid sequence |
US9732381B2 (en) | 2009-03-25 | 2017-08-15 | Oxford University Innovation Limited | Method for sequencing a heteropolymeric target nucleic acid sequence |
US10669581B2 (en) | 2009-03-25 | 2020-06-02 | Oxford University Innovation Limited | Method for sequencing a heteropolymeric target nucleic acid sequence |
WO2010109197A3 (en) * | 2009-03-25 | 2011-03-17 | Isis Innovation Limited | Method for sequencing a heteropolymeric target nucleic acid sequence |
WO2011067559A1 (en) | 2009-12-01 | 2011-06-09 | Oxford Nanopore Technologies Limited | Biochemical analysis instrument |
WO2012042226A2 (en) | 2010-10-01 | 2012-04-05 | Oxford Nanopore Technologies Limited | Biochemical analysis apparatus and rotary valve |
US9751915B2 (en) | 2011-02-11 | 2017-09-05 | Oxford Nanopore Technologies Ltd. | Mutant pores |
US11136623B2 (en) | 2011-05-27 | 2021-10-05 | Oxford Nanopore Technologies Limited | Coupling method |
US11041194B2 (en) | 2011-05-27 | 2021-06-22 | Oxford Nanopore Technologies Ltd. | Coupling method |
US11946102B2 (en) | 2011-05-27 | 2024-04-02 | Oxford Nanopore Technologies Plc | Coupling method |
US11959135B2 (en) | 2011-05-27 | 2024-04-16 | Oxford Nanopore Technologies Plc | Coupling method |
US11168363B2 (en) | 2011-07-25 | 2021-11-09 | Oxford Nanopore Technologies Ltd. | Hairpin loop method for double strand polynucleotide sequencing using transmembrane pores |
US9957560B2 (en) | 2011-07-25 | 2018-05-01 | Oxford Nanopore Technologies Ltd. | Hairpin loop method for double strand polynucleotide sequencing using transmembrane pores |
US11261487B2 (en) | 2011-07-25 | 2022-03-01 | Oxford Nanopore Technologies Plc | Hairpin loop method for double strand polynucleotide sequencing using transmembrane pores |
US10851409B2 (en) | 2011-07-25 | 2020-12-01 | Oxford Nanopore Technologies Ltd. | Hairpin loop method for double strand polynucleotide sequencing using transmembrane pores |
US10597713B2 (en) | 2011-07-25 | 2020-03-24 | Oxford Nanopore Technologies Ltd. | Hairpin loop method for double strand polynucleotide sequencing using transmembrane pores |
US10724087B2 (en) | 2011-10-21 | 2020-07-28 | Oxford Nanopore Technologies Ltd. | Enzyme method |
US11634763B2 (en) | 2011-10-21 | 2023-04-25 | Oxford Nanopore Technologies Plc | Enzyme method |
US9758823B2 (en) | 2011-10-21 | 2017-09-12 | Oxford Nanopore Technologies Limited | Enzyme method |
WO2013057495A3 (en) * | 2011-10-21 | 2013-08-22 | Oxford Nanopore Technologies Limited | Method of characterizing a target polynucleotide using a pore and a hel308 helicase |
US9617591B2 (en) | 2011-12-29 | 2017-04-11 | Oxford Nanopore Technologies Ltd. | Method for characterising a polynucleotide by using a XPD helicase |
US10385382B2 (en) | 2011-12-29 | 2019-08-20 | Oxford Nanopore Technologies Ltd. | Enzyme method |
US10882889B2 (en) | 2012-04-10 | 2021-01-05 | Oxford Nanopore Technologies Ltd. | Mutant lysenin pores |
US11845780B2 (en) | 2012-04-10 | 2023-12-19 | Oxford Nanopore Technologies Plc | Mutant lysenin pores |
US9777049B2 (en) | 2012-04-10 | 2017-10-03 | Oxford Nanopore Technologies Ltd. | Mutant lysenin pores |
WO2014013259A1 (en) * | 2012-07-19 | 2014-01-23 | Oxford Nanopore Technologies Limited | Ssb method |
US11155860B2 (en) | 2012-07-19 | 2021-10-26 | Oxford Nanopore Technologies Ltd. | SSB method |
US9797009B2 (en) | 2012-07-19 | 2017-10-24 | Oxford Nanopore Technologies Limited | Enzyme construct |
US11525126B2 (en) | 2012-07-19 | 2022-12-13 | Oxford Nanopore Technologies Plc | Modified helicases |
US10808231B2 (en) | 2012-07-19 | 2020-10-20 | Oxford Nanopore Technologies Limited | Modified helicases |
WO2014013262A1 (en) * | 2012-07-19 | 2014-01-23 | Oxford Nanopore Technologies Limited | Enzyme construct |
US11560589B2 (en) | 2013-03-08 | 2023-01-24 | Oxford Nanopore Technologies Plc | Enzyme stalling method |
US10221450B2 (en) | 2013-03-08 | 2019-03-05 | Oxford Nanopore Technologies Ltd. | Enzyme stalling method |
US10976311B2 (en) | 2013-03-25 | 2021-04-13 | Katholieke Universiteit Leuven | Nanopore biosensors for detection of proteins and nucleic acids |
US10514378B2 (en) | 2013-03-25 | 2019-12-24 | Katholieke Universiteit Leuven | Nanopore biosensors for detection of proteins and nucleic acids |
US11761956B2 (en) | 2013-03-25 | 2023-09-19 | Katholieke Universiteit Leuven | Nanopore biosensors for detection of proteins and nucleic acids |
US10802015B2 (en) | 2013-03-25 | 2020-10-13 | Katholieke Universiteit Leuven | Nanopore biosensors for detection of proteins and nucleic acids |
US10006905B2 (en) | 2013-03-25 | 2018-06-26 | Katholieke Universiteit Leuven | Nanopore biosensors for detection of proteins and nucleic acids |
US11186857B2 (en) | 2013-08-16 | 2021-11-30 | Oxford Nanopore Technologies Plc | Polynucleotide modification methods |
US10501767B2 (en) | 2013-08-16 | 2019-12-10 | Oxford Nanopore Technologies Ltd. | Polynucleotide modification methods |
US10724018B2 (en) | 2013-10-18 | 2020-07-28 | Oxford Nanopore Technologies Ltd. | Modified helicases |
US11525125B2 (en) | 2013-10-18 | 2022-12-13 | Oxford Nanopore Technologies Plc | Modified helicases |
US10385389B2 (en) | 2014-01-22 | 2019-08-20 | Oxford Nanopore Technologies Ltd. | Method for attaching one or more polynucleotide binding proteins to a target polynucleotide |
US11725235B2 (en) | 2014-01-22 | 2023-08-15 | Oxford Nanopore Technologies Plc | Method for attaching one or more polynucleotide binding proteins to a target polynucleotide |
US10392658B2 (en) | 2014-01-22 | 2019-08-27 | Oxford Nanopore Technologies Ltd. | Method for controlling the movement of a polynucleotide through a transmembrane pore |
US10669578B2 (en) | 2014-02-21 | 2020-06-02 | Oxford Nanopore Technologies Ltd. | Sample preparation method |
US11542551B2 (en) | 2014-02-21 | 2023-01-03 | Oxford Nanopore Technologies Plc | Sample preparation method |
US11236385B2 (en) | 2014-04-04 | 2022-02-01 | Oxford Nanopore Technologies Ltd. | Method for characterising a double stranded nucleic acid using a nano-pore and anchor molecules at both ends of said nucleic acid |
US10337060B2 (en) | 2014-04-04 | 2019-07-02 | Oxford Nanopore Technologies Ltd. | Method for characterising a double stranded nucleic acid using a nano-pore and anchor molecules at both ends of said nucleic acid |
US10774378B2 (en) | 2014-04-04 | 2020-09-15 | Oxford Nanopore Technologies Ltd. | Method of target molecule characterisation using a molecular pore |
US11649490B2 (en) | 2014-04-04 | 2023-05-16 | Oxford Nanopore Technologies Plc | Method of target molecule characterisation using a molecular pore |
US10167503B2 (en) | 2014-05-02 | 2019-01-01 | Oxford Nanopore Technologies Ltd. | Mutant pores |
US10443097B2 (en) | 2014-05-02 | 2019-10-15 | Oxford Nanopore Technologies Ltd. | Method of improving the movement of a target polynucleotide with respect to a transmembrane pore |
US10844432B2 (en) | 2014-05-02 | 2020-11-24 | Oxford Nanopore Technologies, Ltd. | Method of improving the movement of a target polynucleotide with respect to a transmembrane pore |
US11739377B2 (en) | 2014-05-02 | 2023-08-29 | Oxford Nanopore Technologies Plc | Method of improving the movement of a target polynucleotide with respect to a transmembrane pore |
US10948455B2 (en) | 2014-06-25 | 2021-03-16 | Huldagate Technologies Limited | Sequencer |
US10400014B2 (en) | 2014-09-01 | 2019-09-03 | Oxford Nanopore Technologies Ltd. | Mutant CsgG pores |
US11034734B2 (en) | 2014-09-01 | 2021-06-15 | Oxford Nanopore Technologies Ltd. | Mutant pores |
US11180741B2 (en) | 2014-10-07 | 2021-11-23 | Oxford Nanopore Technologies Ltd. | Modified enzymes |
US11965183B2 (en) | 2014-10-07 | 2024-04-23 | Oxford Nanopore Technologies Plc | Modified enzymes |
US10266885B2 (en) | 2014-10-07 | 2019-04-23 | Oxford Nanopore Technologies Ltd. | Mutant pores |
US11390904B2 (en) | 2014-10-14 | 2022-07-19 | Oxford Nanopore Technologies Plc | Nanopore-based method and double stranded nucleic acid construct therefor |
US10570440B2 (en) | 2014-10-14 | 2020-02-25 | Oxford Nanopore Technologies Ltd. | Method for modifying a template double stranded polynucleotide using a MuA transposase |
US11613771B2 (en) | 2014-10-17 | 2023-03-28 | Oxford Nanopore Technologies Plc | Methods for delivering an analyte to transmembrane pores |
US10760114B2 (en) | 2014-10-17 | 2020-09-01 | Oxford Nanopore Technologies Ltd. | Methods for delivering an analyte to transmembrane pores |
US11479584B2 (en) | 2014-10-31 | 2022-10-25 | Roche Sequencing Solutions, Inc. | Alpha-hemolysin variants with altered characteristics |
US9557294B2 (en) | 2014-12-19 | 2017-01-31 | Genia Technologies, Inc. | Nanopore-based sequencing with varying voltage stimulus |
US9863904B2 (en) | 2014-12-19 | 2018-01-09 | Genia Technologies, Inc. | Nanopore-based sequencing with varying voltage stimulus |
US10545112B2 (en) | 2014-12-19 | 2020-01-28 | Roche Sequencing Solutions, Inc. | Nanopore-based sequencing with varying voltage stimulus |
US11307192B2 (en) | 2015-02-19 | 2022-04-19 | Oxford Nanopore Technologies Plc | Method for producing a hetero-oligomeric pore comprising two different monomers in a specific stiochiometric ratio |
US10472673B2 (en) | 2015-02-19 | 2019-11-12 | Oxford Nanopore Technologies Ltd. | Hetero-pores |
US11169138B2 (en) | 2015-04-14 | 2021-11-09 | Katholieke Universiteit Leuven | Nanopores with internal protein adaptors |
US11261488B2 (en) | 2015-09-24 | 2022-03-01 | Roche Sequencing Solutions, Inc. | Alpha-hemolysin variants |
US10670549B2 (en) | 2015-09-24 | 2020-06-02 | Roche Sequencing Solutions, Inc. | Differential output of analog memories storing nanopore measurement samples |
US10126262B2 (en) | 2015-09-24 | 2018-11-13 | Genia Technologies, Inc. | Differential output of analog memories storing nanopore measurement samples |
US10976300B2 (en) | 2015-12-08 | 2021-04-13 | Katholieke Universiteit Leuven | Modified nanopores, compositions comprising the same, and uses thereof |
US11597970B2 (en) | 2016-03-02 | 2023-03-07 | Oxford Nanopore Technologies Plc | Mutant pores |
US11186868B2 (en) | 2016-03-02 | 2021-11-30 | Oxford Nanopore Technologies Plc | Mutant pore |
US11685949B2 (en) | 2016-03-02 | 2023-06-27 | Oxford Nanopore Technologies Plc | Mutant pore |
US10975428B2 (en) | 2016-03-02 | 2021-04-13 | Oxford Nanopore Technologies Ltd. | Mutant pore |
US10995372B2 (en) | 2016-03-02 | 2021-05-04 | Oxford Nanopore Technologies Ltd. | Mutant pores |
US11104709B2 (en) | 2016-04-06 | 2021-08-31 | Oxford Nanopore Technologies Ltd. | Mutant pore |
US11939359B2 (en) | 2016-04-06 | 2024-03-26 | Oxford Nanopore Technologies Plc | Mutant pore |
US11649480B2 (en) | 2016-05-25 | 2023-05-16 | Oxford Nanopore Technologies Plc | Method for modifying a template double stranded polynucleotide |
US11752497B2 (en) * | 2017-04-19 | 2023-09-12 | Electronic Biosciences, Inc. | Nanopore/nanowell electrode enabled exonuclease sequencing |
US20200171487A1 (en) * | 2017-04-19 | 2020-06-04 | Electronic Biosciences, Inc. | Nanopore/nanowell electrode enabled exonuclease sequencing |
US11572387B2 (en) | 2017-06-30 | 2023-02-07 | Vib Vzw | Protein pores |
US11945840B2 (en) | 2017-06-30 | 2024-04-02 | Vib Vzw | Protein pores |
US11639524B2 (en) | 2017-11-27 | 2023-05-02 | Roche Sequencing Solutions, Inc. | Normalization and baseline shift removal by rotation in added data dimensions |
US11725205B2 (en) | 2018-05-14 | 2023-08-15 | Oxford Nanopore Technologies Plc | Methods and polynucleotides for amplifying a target polynucleotide |
WO2023161625A1 (en) | 2022-02-28 | 2023-08-31 | Oxford Nanopore Technologies Plc | Apparatus and methods for controlling insertion of a membrane channel into a membrane |
Also Published As
Publication number | Publication date |
---|---|
CN101356288B (en) | 2016-02-03 |
AU2006314296B2 (en) | 2012-08-23 |
US20080311582A1 (en) | 2008-12-18 |
NZ568163A (en) | 2011-11-25 |
MY149161A (en) | 2013-07-31 |
RU2008123842A (en) | 2009-12-27 |
GB0523282D0 (en) | 2005-12-21 |
EP2272981A1 (en) | 2011-01-12 |
CA2629009A1 (en) | 2007-05-24 |
CA2629009C (en) | 2016-02-02 |
KR20080083281A (en) | 2008-09-17 |
EP1951898A1 (en) | 2008-08-06 |
UA95087C2 (en) | 2011-07-11 |
AU2006314296A1 (en) | 2007-05-24 |
JP2009519705A (en) | 2009-05-21 |
US20120219960A1 (en) | 2012-08-30 |
ATE545712T1 (en) | 2012-03-15 |
US8105846B2 (en) | 2012-01-31 |
JP5328362B2 (en) | 2013-10-30 |
CN101356288A (en) | 2009-01-28 |
IL191319A (en) | 2011-10-31 |
EP1951898B1 (en) | 2012-02-15 |
US8785211B2 (en) | 2014-07-22 |
EP2272981B1 (en) | 2013-01-09 |
BRPI0618391A2 (en) | 2011-08-30 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
US8105846B2 (en) | Methods using pores | |
US20200407785A1 (en) | Method for sequencing a heteropolymeric target nucleic acid sequence | |
EP2350122B1 (en) | Methods of enhancing translocation of charged analytes through transmembrane protein pores | |
KR102106499B1 (en) | Analysis of measurements of a polymer | |
US8822160B2 (en) | Molecular adaptors | |
EP2310534B1 (en) | Base-detecting pore | |
WO2012095660A2 (en) | Method using fluorinated amphiphiles | |
MX2008006277A (en) | Methods using pores |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
121 | Ep: the epo has been informed by wipo that ep was designated in this application | ||
WWE | Wipo information: entry into national phase |
Ref document number: 191319 Country of ref document: IL Ref document number: 3911/DELNP/2008 Country of ref document: IN |
|
WWE | Wipo information: entry into national phase |
Ref document number: 2629009 Country of ref document: CA |
|
WWE | Wipo information: entry into national phase |
Ref document number: 568163 Country of ref document: NZ |
|
WWE | Wipo information: entry into national phase |
Ref document number: 2006314296 Country of ref document: AU |
|
ENP | Entry into the national phase |
Ref document number: 2008540686 Country of ref document: JP Kind code of ref document: A |
|
WWE | Wipo information: entry into national phase |
Ref document number: MX/a/2008/006277 Country of ref document: MX |
|
NENP | Non-entry into the national phase |
Ref country code: DE |
|
ENP | Entry into the national phase |
Ref document number: 2006314296 Country of ref document: AU Date of ref document: 20061115 Kind code of ref document: A |
|
WWP | Wipo information: published in national office |
Ref document number: 2006314296 Country of ref document: AU |
|
WWE | Wipo information: entry into national phase |
Ref document number: 2006808555 Country of ref document: EP |
|
WWE | Wipo information: entry into national phase |
Ref document number: 1020087014358 Country of ref document: KR |
|
WWE | Wipo information: entry into national phase |
Ref document number: 2008123842 Country of ref document: RU |
|
WWE | Wipo information: entry into national phase |
Ref document number: 200680050878.X Country of ref document: CN |
|
WWE | Wipo information: entry into national phase |
Ref document number: 12093610 Country of ref document: US |
|
WWP | Wipo information: published in national office |
Ref document number: 2006808555 Country of ref document: EP |
|
ENP | Entry into the national phase |
Ref document number: PI0618391 Country of ref document: BR Kind code of ref document: A2 Effective date: 20080513 |