WO2007055735A3 - Hydrolases, nucleic acids encoding them and methods for making and using them - Google Patents

Hydrolases, nucleic acids encoding them and methods for making and using them Download PDF

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Publication number
WO2007055735A3
WO2007055735A3 PCT/US2006/020169 US2006020169W WO2007055735A3 WO 2007055735 A3 WO2007055735 A3 WO 2007055735A3 US 2006020169 W US2006020169 W US 2006020169W WO 2007055735 A3 WO2007055735 A3 WO 2007055735A3
Authority
WO
WIPO (PCT)
Prior art keywords
activity
polypeptides
hydrolase
making
phospholipase
Prior art date
Application number
PCT/US2006/020169
Other languages
French (fr)
Other versions
WO2007055735A2 (en
Inventor
Eric Mathur
Walter Callen
Roderick Fielding
Original Assignee
Verenium Corp
Eric Mathur
Walter Callen
Roderick Fielding
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Verenium Corp, Eric Mathur, Walter Callen, Roderick Fielding filed Critical Verenium Corp
Priority to EP06844108A priority Critical patent/EP1910530A2/en
Priority to CA002611071A priority patent/CA2611071A1/en
Publication of WO2007055735A2 publication Critical patent/WO2007055735A2/en
Publication of WO2007055735A3 publication Critical patent/WO2007055735A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Cosmetics (AREA)

Abstract

The invention provides hydrolases, polynucleotides encoding them, and methods of making and using these polynucleotides and polypeptides. In one aspect, the invention is directed to polypeptides, e.g., enzymes, having a hydrolase activity, e.g., an esterase, acylase, lipase, phospholipase (e.g., phospholipase A, B, C and/or D activity, patatin activity, lipid acyl hydrolase (LAH) activity) or protease activity, including thermostable and thermotolerant hydrolase activity, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides. The hydrolase activities of the polypeptides and peptides of the invention include esterase activity, lipase activity (hydrolysis of lipids), acidolysis reactions (to replace an esterified fatty acid with a free fatty acid), transesterification reactions (exchange of fatty acids between triglycerides), ester synthesis, ester interchange reactions, phospholipase activity and protease activity (hydrolysis of peptide bonds). The polypeptides of the invention can be used in a variety of pharmaceutical, agricultural and industrial contexts, including the manufacture of cosmetics and nutraceuticals. In another aspect, the polypeptides of the invention are used to synthesize enantiomerically pure chiral products.
PCT/US2006/020169 2005-05-31 2006-05-23 Hydrolases, nucleic acids encoding them and methods for making and using them WO2007055735A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP06844108A EP1910530A2 (en) 2005-05-31 2006-05-23 Hydrolases, nucleic acids encoding them and methods for making and using them
CA002611071A CA2611071A1 (en) 2005-05-31 2006-05-23 Hydrolases, nucleic acids encoding them and methods for making and using them

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US68602005P 2005-05-31 2005-05-31
US60/686,020 2005-05-31

Publications (2)

Publication Number Publication Date
WO2007055735A2 WO2007055735A2 (en) 2007-05-18
WO2007055735A3 true WO2007055735A3 (en) 2008-10-02

Family

ID=38023721

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2006/020169 WO2007055735A2 (en) 2005-05-31 2006-05-23 Hydrolases, nucleic acids encoding them and methods for making and using them

Country Status (3)

Country Link
EP (1) EP1910530A2 (en)
CA (1) CA2611071A1 (en)
WO (1) WO2007055735A2 (en)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BRPI0815860B1 (en) 2007-09-12 2021-04-20 Dsm Ip Assets B.V. methods to produce a biological oil
US20130059036A1 (en) 2010-03-12 2013-03-07 Dupont Nutrition Biosciences Aps Process
CA2798152A1 (en) 2010-06-17 2011-12-22 Dupont Nutrition Biosciences Aps Process
WO2012114232A1 (en) 2011-02-23 2012-08-30 Dupont Nutrition Biosciences Aps Process
DK2678438T3 (en) 2011-02-23 2017-10-02 Dupont Nutrition Biosci Aps ENZYME TREATMENT OF CHLOROPHYL IN PLANT OILS
US8268305B1 (en) 2011-09-23 2012-09-18 Bio-Cat, Inc. Method and compositions to reduce serum levels of triacylglycerides in human beings using a fungal lipase
WO2013104660A1 (en) 2012-01-13 2013-07-18 Dupont Nutrition Biosciences Aps Process for treating a plant oil comprising hydrolysing chlorophyll or a chlorophyll derivative and involving partial caustic neutralisation
WO2013160372A1 (en) 2012-04-27 2013-10-31 Dupont Nutrition Biosciences Aps Process for treating plant oil involving addition of serial doses of chlorophyll or chlorophyll derivative degrading enzyme
EP3164498A4 (en) 2014-07-03 2018-02-14 Sustainable Bioproducts Holdings, LLC Acidophilic fusarium oxysporum strains, methods of their production and methods of their use
CN104715320B (en) * 2015-01-22 2017-02-22 青岛大学 Method for comprehensively evaluating quality of spinning raw material
SG10201911169TA (en) 2016-03-01 2020-01-30 Sustainable Bioproducts Inc Filamentous fungal biomats, methods of their production and methods of their use
IL272918B2 (en) 2017-08-30 2024-02-01 The Fynder Group Inc Edible composition with filamentous fungi and bioreactor system for the cultivation thereof
CA3108587A1 (en) 2019-02-27 2020-09-03 The Fynder Group, Inc. Stable foam comprising filamentous fungal particles
CA3143603A1 (en) 2019-06-18 2020-12-24 The Fynder Group, Inc. Fungal textile materials and leather analogs
CN116024097B (en) * 2022-08-30 2023-11-21 四川农业大学 Rhizopus delemar SICAU-Z1 and application thereof

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040005604A1 (en) * 2002-04-19 2004-01-08 Diversa Corporation Phospholipases, nucleic acids encoding them and methods for making and using them
WO2005032496A2 (en) * 2003-03-07 2005-04-14 Diversa Corporation Hydrolases, nucleic acids encoding them and mehods for making and using them

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040005604A1 (en) * 2002-04-19 2004-01-08 Diversa Corporation Phospholipases, nucleic acids encoding them and methods for making and using them
WO2005032496A2 (en) * 2003-03-07 2005-04-14 Diversa Corporation Hydrolases, nucleic acids encoding them and mehods for making and using them

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
ROZENGURT E.: "Taste receptor signaling in the mamalian gut", CURRENT OPINION IN PHARMACOLOGY, vol. 7, 2007, pages 557 - 562, XP022371168 *

Also Published As

Publication number Publication date
CA2611071A1 (en) 2007-05-18
EP1910530A2 (en) 2008-04-16
WO2007055735A2 (en) 2007-05-18

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