WO2007050040A8 - Immobilization unit and device for isolation of nucleic acid molecules - Google Patents

Immobilization unit and device for isolation of nucleic acid molecules

Info

Publication number
WO2007050040A8
WO2007050040A8 PCT/SG2005/000374 SG2005000374W WO2007050040A8 WO 2007050040 A8 WO2007050040 A8 WO 2007050040A8 SG 2005000374 W SG2005000374 W SG 2005000374W WO 2007050040 A8 WO2007050040 A8 WO 2007050040A8
Authority
WO
WIPO (PCT)
Prior art keywords
immobilization unit
isolation
nucleic acid
acid molecules
channel
Prior art date
Application number
PCT/SG2005/000374
Other languages
French (fr)
Other versions
WO2007050040A1 (en
Inventor
Hongmiao Ji
Levent Yobas
Yu Chen
Wing Cheong Hui
Chew Kiat Heng
Tit Meng Lim
Original Assignee
Agency Science Tech & Res
Univ Singapore
Hongmiao Ji
Levent Yobas
Yu Chen
Wing Cheong Hui
Chew Kiat Heng
Tit Meng Lim
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Agency Science Tech & Res, Univ Singapore, Hongmiao Ji, Levent Yobas, Yu Chen, Wing Cheong Hui, Chew Kiat Heng, Tit Meng Lim filed Critical Agency Science Tech & Res
Priority to PCT/SG2005/000374 priority Critical patent/WO2007050040A1/en
Publication of WO2007050040A1 publication Critical patent/WO2007050040A1/en
Publication of WO2007050040A8 publication Critical patent/WO2007050040A8/en

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502746—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means for controlling flow resistance, e.g. flow controllers, baffles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F25/00—Flow mixers; Mixers for falling materials, e.g. solid particles
    • B01F25/40—Static mixers
    • B01F25/42—Static mixers in which the mixing is affected by moving the components jointly in changing directions, e.g. in tubes provided with baffles or obstructions
    • B01F25/43—Mixing tubes, e.g. wherein the material is moved in a radial or partly reversed direction
    • B01F25/433—Mixing tubes wherein the shape of the tube influences the mixing, e.g. mixing tubes with varying cross-section or provided with inwardly extending profiles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F25/00—Flow mixers; Mixers for falling materials, e.g. solid particles
    • B01F25/40—Static mixers
    • B01F25/42—Static mixers in which the mixing is affected by moving the components jointly in changing directions, e.g. in tubes provided with baffles or obstructions
    • B01F25/43—Mixing tubes, e.g. wherein the material is moved in a radial or partly reversed direction
    • B01F25/433—Mixing tubes wherein the shape of the tube influences the mixing, e.g. mixing tubes with varying cross-section or provided with inwardly extending profiles
    • B01F25/4331—Mixers with bended, curved, coiled, wounded mixing tubes or comprising elements for bending the flow
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F33/00—Other mixers; Mixing plants; Combinations of mixers
    • B01F33/30—Micromixers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/34—Purifying; Cleaning
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0631—Purification arrangements, e.g. solid phase extraction [SPE]
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0681—Filter
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0867—Multiple inlets and one sample wells, e.g. mixing, dilution
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N1/405—Concentrating samples by adsorption or absorption

Landscapes

  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Health & Medical Sciences (AREA)
  • Dispersion Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Clinical Laboratory Science (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

An immobilization unit (820) for the detection of dexoyribonucleic acid (DNA) and ribonucleic acid (RNA) molecules capable of immobilizing nucleic molecules, said immobilization unit comprising a channel of a given depth, width and length and having at least one surface. The surface is adapted to immobilize nucleic molecules under suitable conditions. The channel has at least one straight portion and at least one meandering portion. The at least one meandering portion is essentially u-shaped and the at least one straight portion is an arm of the u-shaped meandering portion.
PCT/SG2005/000374 2005-10-28 2005-10-28 Immobilization unit and device for isolation of nucleic acid molecules WO2007050040A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
PCT/SG2005/000374 WO2007050040A1 (en) 2005-10-28 2005-10-28 Immobilization unit and device for isolation of nucleic acid molecules

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/SG2005/000374 WO2007050040A1 (en) 2005-10-28 2005-10-28 Immobilization unit and device for isolation of nucleic acid molecules

Publications (2)

Publication Number Publication Date
WO2007050040A1 WO2007050040A1 (en) 2007-05-03
WO2007050040A8 true WO2007050040A8 (en) 2007-08-23

Family

ID=37968070

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/SG2005/000374 WO2007050040A1 (en) 2005-10-28 2005-10-28 Immobilization unit and device for isolation of nucleic acid molecules

Country Status (1)

Country Link
WO (1) WO2007050040A1 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009113010A1 (en) * 2008-03-13 2009-09-17 Nxp B.V. A sensor device and a method of detecting compounds, particles or complexes
WO2012170560A2 (en) * 2011-06-06 2012-12-13 Cornell University Microfluidic device for extracting, isolating, and analyzing dna from cells
US11383240B2 (en) 2016-05-22 2022-07-12 Cornell University Single cell whole genome amplification via micropillar arrays under flow conditions
BE1026469B1 (en) * 2018-07-13 2020-02-11 Van Lidth De Jeude Jehan Lienart MICROFLUIDIC DEVICE FOR SELECTING BACTERIOPHAGES CAPABLE OF INFECTING BACTERIA CONTAINED IN A SAMPLE

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5842787A (en) * 1997-10-09 1998-12-01 Caliper Technologies Corporation Microfluidic systems incorporating varied channel dimensions
DE19819537A1 (en) * 1998-04-30 2000-03-16 Biochip Technologies Gmbh Analysis and diagnostic tool
CA2409826C (en) * 2000-06-01 2010-02-02 Science Applications International Corporation Systems and methods for monitoring health and delivering drugs transdermally
AU2001297830A1 (en) * 2000-10-25 2002-12-09 Exiqon A/S Closed substrate platforms suitable for analysis of biomolecules
WO2002052045A1 (en) * 2000-12-26 2002-07-04 Aviva Biosciences Active and biocompatible platforms prepared by polymerization of surface coating films
US20030175947A1 (en) * 2001-11-05 2003-09-18 Liu Robin Hui Enhanced mixing in microfluidic devices
US7186383B2 (en) * 2002-09-27 2007-03-06 Ast Management Inc. Miniaturized fluid delivery and analysis system
US20050074900A1 (en) * 2003-10-07 2005-04-07 Morgan Nicole Y. Microfluidic flow-through immunoassay for simultaneous detection of multiple proteins in a biological sample
US20050095602A1 (en) * 2003-11-04 2005-05-05 West Jason A. Microfluidic integrated microarrays for biological detection

Also Published As

Publication number Publication date
WO2007050040A1 (en) 2007-05-03

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