WO2007048629A3 - Modulation of rna silencing efficiency by argonaute proteins - Google Patents

Modulation of rna silencing efficiency by argonaute proteins Download PDF

Info

Publication number
WO2007048629A3
WO2007048629A3 PCT/EP2006/010373 EP2006010373W WO2007048629A3 WO 2007048629 A3 WO2007048629 A3 WO 2007048629A3 EP 2006010373 W EP2006010373 W EP 2006010373W WO 2007048629 A3 WO2007048629 A3 WO 2007048629A3
Authority
WO
WIPO (PCT)
Prior art keywords
modulation
rna silencing
silencing efficiency
argonaute proteins
rna
Prior art date
Application number
PCT/EP2006/010373
Other languages
French (fr)
Other versions
WO2007048629A2 (en
Inventor
Volker Patzel
Stefan H E Kaufmann
Original Assignee
Max Planck Gesellschaft
Volker Patzel
Stefan H E Kaufmann
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Max Planck Gesellschaft, Volker Patzel, Stefan H E Kaufmann filed Critical Max Planck Gesellschaft
Publication of WO2007048629A2 publication Critical patent/WO2007048629A2/en
Publication of WO2007048629A3 publication Critical patent/WO2007048629A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/14—Type of nucleic acid interfering N.A.
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2320/00—Applications; Uses
    • C12N2320/50—Methods for regulating/modulating their activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2330/00—Production
    • C12N2330/30—Production chemically synthesised

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Optical Modulation, Optical Deflection, Nonlinear Optics, Optical Demodulation, Optical Logic Elements (AREA)

Abstract

The present invention relates to methods and compositions for modulating RNA silencing efficiency by providing selective RISC (RNA-induced silencing complex) formation.
PCT/EP2006/010373 2005-10-28 2006-10-27 Modulation of rna silencing efficiency by argonaute proteins WO2007048629A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP05023703 2005-10-28
EP05023703.1 2005-10-28

Publications (2)

Publication Number Publication Date
WO2007048629A2 WO2007048629A2 (en) 2007-05-03
WO2007048629A3 true WO2007048629A3 (en) 2007-11-15

Family

ID=37968179

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2006/010373 WO2007048629A2 (en) 2005-10-28 2006-10-27 Modulation of rna silencing efficiency by argonaute proteins

Country Status (1)

Country Link
WO (1) WO2007048629A2 (en)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101932708A (en) * 2007-08-14 2010-12-29 联邦科学工业研究组织 Improved gene silencing methods
DE102009024730A1 (en) * 2009-06-12 2010-12-16 Universitätsklinikum Schleswig-Holstein Campus Lübeck New oligonucleotide, comprising specified sequences, is argonaute-3a expression inhibitor, useful for diagnosing or treating disorders or diseases e.g. angiogenesis, hematopoiesis, embryogenesis or microRNA-biogenesis
WO2015157534A1 (en) * 2014-04-10 2015-10-15 The Regents Of The University Of California Methods and compositions for using argonaute to modify a single stranded target nucleic acid
GB201410693D0 (en) 2014-06-16 2014-07-30 Univ Southampton Splicing modulation
JP6867945B2 (en) 2014-10-03 2021-05-12 コールド スプリング ハーバー ラボラトリー Targeted enhancement of nuclear gene output
WO2016166268A1 (en) * 2015-04-17 2016-10-20 Cellectis Engineering animal or plant genome using dna-guided argonaute interference systems (dais) from mesophilic prokaryotes
JP6923517B2 (en) 2015-10-09 2021-08-18 ユニバーシティ・オブ・サザンプトン Screening for regulatory and deregulated protein expression of gene expression
US11096956B2 (en) 2015-12-14 2021-08-24 Stoke Therapeutics, Inc. Antisense oligomers and uses thereof
JP2018538288A (en) * 2015-12-14 2018-12-27 コールド スプリング ハーバー ラボラトリー Antisense oligomers for the treatment of Alagille syndrome
US11083745B2 (en) 2015-12-14 2021-08-10 Cold Spring Harbor Laboratory Antisense oligomers for treatment of autosomal dominant mental retardation-5 and Dravet Syndrome
US11261483B2 (en) 2016-11-09 2022-03-01 Board Of Trustees Of The University Of Illinois Programmable DNA-guided artificial restriction enzymes
CA3047027A1 (en) * 2016-12-14 2018-06-21 Shanghaitech University Compositions and methods for treating cancer by inhibiting piwil4
WO2018209288A1 (en) * 2017-05-12 2018-11-15 Massachusetts Institute Of Technology Argonaute protein-double stranded rna complexes and uses related thereto
KR102643936B1 (en) 2017-08-25 2024-03-05 스톡 테라퓨틱스, 인크. Antisense oligomers for treatment of conditions and diseases
BR112020022512A2 (en) 2018-05-04 2021-05-04 Stoke Therapeutics, Inc. methods and compositions for treating cholesteryl ester storage disease
WO2019222036A1 (en) 2018-05-18 2019-11-21 Insideoutbio, Inc. Genetically engineered argonaute proteins with enhanced gene silencing activity and methods of use thereof
EP3744849A1 (en) 2019-05-31 2020-12-02 Consejo Superior de Investigaciones Cientificas (CSIC) Method for modulating the gene silencing degree induced by trans-acting small interfering rnas
IL298063A (en) 2020-05-11 2023-01-01 Stoke Therapeutics Inc Opa1 antisense oligomers for treatment of conditions and diseases
US20230242912A1 (en) * 2020-06-30 2023-08-03 Ohio State Innovation Foundation Methods and compositions related to catalytic activation of human argonaute-3
WO2024086805A2 (en) * 2022-10-21 2024-04-25 Watchmaker Genomics, Inc. Methods and compositions for sequencing library normalization

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1666593A1 (en) * 2003-08-18 2006-06-07 Japan Health Science Foundation IMPROVED siRNA MOLECULE AND METHOD OF INHIBITING GENE EXPRESSION WITH THE USE OF THE SAME

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1666593A1 (en) * 2003-08-18 2006-06-07 Japan Health Science Foundation IMPROVED siRNA MOLECULE AND METHOD OF INHIBITING GENE EXPRESSION WITH THE USE OF THE SAME

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
BAUMBERGER N ET AL: "Arabidopsis ARGONAUTE1 is an RNA Slicer that selectively recruits rnicroRNAs and short interfering RNAs", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, vol. 102, no. 33, August 2005 (2005-08-01), pages 11928 - 11933, XP002438536, ISSN: 0027-8424 *
HAMADA M ET AL: "EFFECTS OF RNA INTERFERENCE IN GENE EXPRESSION (RNAI) IN CULTURED MAMMALAIN CELLS OF MISMATCHES AND THE INTRODUCTION OF CHEMICAL MODIFICATIONS AT THE 3'-ENDS OF SIRNAS", ANTISENSE & NUCLEIC ACID DRUG DEVELOPMENT, MARY ANN LIEBERT, INC., NEW YORK, US, vol. 12, no. 5, October 2002 (2002-10-01), pages 301 - 309, XP009006637, ISSN: 1087-2906 *
MANIATAKI ELISAVET ET AL: "A human, ATP-independent, RISC assembly machine fueled by pre-miRNA", GENES & DEVELOPMENT, vol. 19, no. 24, December 2005 (2005-12-01), pages 2979 - 2990, XP002438538, ISSN: 0890-9369 *
OKAMURA KATSUTOMO ET AL: "Distinct roles for argonaute proteins in small RNA-directed RNA cleavage pathways", GENES & DEVELOPMENT, vol. 18, no. 14, 15 July 2004 (2004-07-15), pages 1655 - 1666, XP002438534, ISSN: 0890-9369 *
PATZEL VOLKER ET AL: "Design of siRNAs producing unstructured guide-RNAs results in improved RNA interference efficiency", NATURE BIOTECHNOLOGY, vol. 23, no. 11, November 2005 (2005-11-01), pages 1440 - 1444, XP002438537, ISSN: 1087-0156 *
TOMARI YUKIHIDE ET AL: "A protein sensor for siRNA asymmetry", SCIENCE (WASHINGTON D C), vol. 306, no. 5700, 19 November 2004 (2004-11-19), pages 1377 - 1380, XP002438535, ISSN: 0036-8075 *

Also Published As

Publication number Publication date
WO2007048629A2 (en) 2007-05-03

Similar Documents

Publication Publication Date Title
WO2007048629A3 (en) Modulation of rna silencing efficiency by argonaute proteins
WO2007048628A3 (en) Structures of active guide rna molecules and method of selection
WO2006036916A3 (en) Rnai modulation of apob and uses thereof
WO2006066158A3 (en) Rnai modulation of mll-af4 and uses thereof
WO2005047505A3 (en) Methods and products for expression of micro rnas
WO2006028936A3 (en) Heteromultimeric molecules
IL190154A0 (en) Modulation of immunostimulatory properties of short interfering ribonucleic acid (sirna) by nucleotide modification
WO2009039307A3 (en) Hydrogels and methods for producing and using the same
WO2007036366A8 (en) Modification of rna, producing an increased transcript stability and translation efficiency
WO2008025856A3 (en) Modified glycoproteins
WO2004099249A3 (en) Optimized fc variants and methods for their generation
WO2006119062A3 (en) Sclerostin epitopes
WO2006046148A3 (en) Rna constructs
AU2008257801A8 (en) RSV-specific binding molecules and means for producing them
WO2007053696A3 (en) Rnai inhibition of influenza virus replication
WO2005054494A3 (en) Sequence-specific inhibition of small rna function
WO2010033906A3 (en) Efficient induction of pluripotent stem cells using small molecule compounds
WO2008152131A3 (en) Rnai inhibition of alpha-enac expression
WO2006029258A3 (en) Aptamer medicinal chemistry
WO2007144779A3 (en) Compositions comprising porphyra and methods of making and using thereof
WO2008017074A3 (en) Dyes and precursors and conjugates thereof
WO2010009879A3 (en) Methods for producing paricalcitol
WO2010091308A3 (en) Oligomeric compounds and methods
WO2007106915A3 (en) Antibodies to egfl7 and methods for their use
WO2008011433A3 (en) Styrenated terpene resin as well as methods of making and using the same

Legal Events

Date Code Title Description
NENP Non-entry into the national phase

Ref country code: DE

121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 06818308

Country of ref document: EP

Kind code of ref document: A2

122 Ep: pct application non-entry in european phase

Ref document number: 06818308

Country of ref document: EP

Kind code of ref document: A2