WO2007031273A3 - Artificial immune-reconstituted epidermis equivalents - Google Patents
Artificial immune-reconstituted epidermis equivalents Download PDFInfo
- Publication number
- WO2007031273A3 WO2007031273A3 PCT/EP2006/008872 EP2006008872W WO2007031273A3 WO 2007031273 A3 WO2007031273 A3 WO 2007031273A3 EP 2006008872 W EP2006008872 W EP 2006008872W WO 2007031273 A3 WO2007031273 A3 WO 2007031273A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- immune
- epidermis
- skin
- reconstituted epidermis
- reconstituted
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0697—Artificial constructs associating cells of different lineages, e.g. tissue equivalents
- C12N5/0698—Skin equivalents
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
- A61P17/02—Drugs for dermatological disorders for treating wounds, ulcers, burns, scars, keloids, or the like
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
- G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
- G01N33/5047—Cells of the immune system
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
- G01N33/5082—Supracellular entities, e.g. tissue, organisms
- G01N33/5088—Supracellular entities, e.g. tissue, organisms of vertebrates
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6893—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids related to diseases not provided for elsewhere
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2502/00—Coculture with; Conditioned medium produced by
- C12N2502/09—Coculture with; Conditioned medium produced by epidermal cells, skin cells, oral mucosa cells
- C12N2502/094—Coculture with; Conditioned medium produced by epidermal cells, skin cells, oral mucosa cells keratinocytes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2502/00—Coculture with; Conditioned medium produced by
- C12N2502/11—Coculture with; Conditioned medium produced by blood or immune system cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2502/00—Coculture with; Conditioned medium produced by
- C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
- C12N2502/1323—Adult fibroblasts
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2503/00—Use of cells in diagnostics
- C12N2503/04—Screening or testing on artificial tissues
- C12N2503/06—Screening or testing on artificial skin
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2800/00—Detection or diagnosis of diseases
- G01N2800/20—Dermatological disorders
Abstract
The invention relates to a method of preparation of a novel human immune-reconstituted epidermis equivalent comprising culturing epidermis precursor cells from skin biopsies or outer root sheaths of hair follicles and then co-culturing this epidermis equivalent with human peripheral blood mononuclear cells expressing CD14 on a solid support, and to such novel human immune-reconstituted epidermis equivalent prepared by this method and comprising progeny of human peripheral blood mononuclear cells originally expressing CD14. The invention further relates to the use of these immune-reconstituted epidermis equivalents in assays relating to immune reactivity and skin-damaging properties of immunogenic and potentially harmful substances as well as to the use of these immune-reconstituted epidermis equivalents in immunotherapy of skin lesions, inflammation and skin diseases caused by pathogens or tumors.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP05405543.9 | 2005-09-16 | ||
EP05405543 | 2005-09-16 |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2007031273A2 WO2007031273A2 (en) | 2007-03-22 |
WO2007031273A3 true WO2007031273A3 (en) | 2007-05-03 |
Family
ID=37709597
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/EP2006/008872 WO2007031273A2 (en) | 2005-09-16 | 2006-09-12 | Artificial immune-reconstituted epidermis equivalents |
Country Status (1)
Country | Link |
---|---|
WO (1) | WO2007031273A2 (en) |
Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20040115133A1 (en) | 2000-05-10 | 2004-06-17 | Wermeling Daniel P. | Intranasal opioid compositions |
GB2485816A (en) * | 2010-11-25 | 2012-05-30 | Alcyomics Ltd | In vitro model for the prediction of immunogenicity, hypersensitivity or allergenicity |
FR3132146A1 (en) * | 2022-01-27 | 2023-07-28 | Genoskin | Ex vivo human model intended for the evaluation of the vaccine potential of a composition |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20030228686A1 (en) * | 2002-06-07 | 2003-12-11 | Mattek Corporation | Three dimensional vaginal tissue model containing immune cells |
EP0789074B1 (en) * | 1996-01-23 | 2003-12-17 | L'oreal | Skin equivalent containing Langerhans cells |
-
2006
- 2006-09-12 WO PCT/EP2006/008872 patent/WO2007031273A2/en active Application Filing
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0789074B1 (en) * | 1996-01-23 | 2003-12-17 | L'oreal | Skin equivalent containing Langerhans cells |
US20030228686A1 (en) * | 2002-06-07 | 2003-12-11 | Mattek Corporation | Three dimensional vaginal tissue model containing immune cells |
Non-Patent Citations (4)
Title |
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FACY V ET AL: "Reactivity of Langerhans cells in human reconstructed epidermis to known allergens and UV radiation.", TOXICOLOGY IN VITRO, vol. 19, no. 6, September 2005 (2005-09-01), pages 787 - 795, XP004944806, ISSN: 0887-2333 * |
LIMAT A & HUNZIKER T: "Use of epidermal equivalents generated from follicular outer root sheath cells in vitro and for autologous grafting of chronic wounds.", CELLS, TISSUES, ORGANS, vol. 172, no. 2, 2002, pages 79 - 85, XP008074938, ISSN: 1422-6405 * |
PONEC M: "Skin constructs for replacement of skin tissues for in vitro testing.", ADVANCED DRUG DELIVERY REVIEWS, vol. 54 Suppl 1, 1 November 2002 (2002-11-01), pages S19 - S30, XP002419725, ISSN: 0169-409X * |
SIVARD P ET AL: "In vitro reconstructed mucosa-integrating Langerhans' cells.", EXPERIMENTAL DERMATOLOGY AUG 2003, vol. 12, no. 4, August 2003 (2003-08-01), pages 346 - 355, XP002295352, ISSN: 0906-6705 * |
Also Published As
Publication number | Publication date |
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WO2007031273A2 (en) | 2007-03-22 |
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