WO2007027947A3 - Method of using pore-forming peptides for genetic transformation, protein extraction, and transmembrane transport - Google Patents

Method of using pore-forming peptides for genetic transformation, protein extraction, and transmembrane transport Download PDF

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Publication number
WO2007027947A3
WO2007027947A3 PCT/US2006/034109 US2006034109W WO2007027947A3 WO 2007027947 A3 WO2007027947 A3 WO 2007027947A3 US 2006034109 W US2006034109 W US 2006034109W WO 2007027947 A3 WO2007027947 A3 WO 2007027947A3
Authority
WO
WIPO (PCT)
Prior art keywords
genetic transformation
pore
protein extraction
transmembrane transport
forming peptides
Prior art date
Application number
PCT/US2006/034109
Other languages
French (fr)
Other versions
WO2007027947A2 (en
Inventor
Daniel Yarbrough
Wenyuan Shi
Fengxia Qi
Original Assignee
Univ California
Daniel Yarbrough
Wenyuan Shi
Fengxia Qi
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ California, Daniel Yarbrough, Wenyuan Shi, Fengxia Qi filed Critical Univ California
Priority to EP06790125A priority Critical patent/EP1929022A4/en
Priority to CA002620935A priority patent/CA2620935A1/en
Publication of WO2007027947A2 publication Critical patent/WO2007027947A2/en
Publication of WO2007027947A3 publication Critical patent/WO2007027947A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4723—Cationic antimicrobial peptides, e.g. defensins

Abstract

There are currently few effective approaches for the non-specific transport of molecules across cell membranes. The present application discloses methods of introducing molecules into and extracting molecules out of a cell in a non-specific manner using antimicrobial peptides. Further, the application discloses methods of utilizing antimicrobial peptides to enhance the competence of bacterial cells that have been rendered competent for genetic transformation.
PCT/US2006/034109 2005-09-01 2006-08-30 Method of using pore-forming peptides for genetic transformation, protein extraction, and transmembrane transport WO2007027947A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP06790125A EP1929022A4 (en) 2005-09-01 2006-08-30 Method of using pore-forming peptides for genetic transformation, protein extraction, and transmembrane transport
CA002620935A CA2620935A1 (en) 2005-09-01 2006-08-30 Method of using pore-forming peptides for genetic transformation, protein extraction, and transmembrane transport

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US71394105P 2005-09-01 2005-09-01
US60/713,941 2005-09-01

Publications (2)

Publication Number Publication Date
WO2007027947A2 WO2007027947A2 (en) 2007-03-08
WO2007027947A3 true WO2007027947A3 (en) 2008-01-10

Family

ID=37809534

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2006/034109 WO2007027947A2 (en) 2005-09-01 2006-08-30 Method of using pore-forming peptides for genetic transformation, protein extraction, and transmembrane transport

Country Status (3)

Country Link
EP (1) EP1929022A4 (en)
CA (1) CA2620935A1 (en)
WO (1) WO2007027947A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2548767T3 (en) 2007-01-16 2015-10-20 The Regents Of The University Of California New antimicrobial peptides

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030186916A1 (en) * 2002-03-12 2003-10-02 Lei Yu Vector for transfection of eukaryotic cells

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7208314B2 (en) * 2002-02-26 2007-04-24 Mirus Bio Corporation Compositions and methods for drug delivery using pH sensitive molecules

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030186916A1 (en) * 2002-03-12 2003-10-02 Lei Yu Vector for transfection of eukaryotic cells

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
GRIGORIEV ET AL.: "Differences in membrane pore formation by peptaibols", JOURNAL OF PEPTIDE SCIENCE, vol. 9, 2003, pages 763 - 768 *
HUANG ET AL.: "Molecular mechanism of peptide-induced pores in membranes", PHYSICAL REVIEW LETTERS, vol. 92, no. 19, May 2004 (2004-05-01), pages 198304-1 - 198304-4 *
QUI ET AL.: "An engineered multidomain bactericidal peptide as a model for targeted antibiotics against specific bacteria", NATURE BIOTECHNOLOGY, vol. 21, no. 12, December 2003 (2003-12-01), pages 1480 - 1485, XP003010122, DOI: doi:10.1038/nbt913 *
SAL-MAN ET AL.: "Preassembly for membrane peptides is an important factor in their selectivity toward target cells", BIOCHEMISTRY, vol. 41, 2002, pages 11921 - 11930 *
See also references of EP1929022A4 *

Also Published As

Publication number Publication date
WO2007027947A2 (en) 2007-03-08
CA2620935A1 (en) 2007-03-08
EP1929022A2 (en) 2008-06-11
EP1929022A4 (en) 2009-05-13

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