WO2007027226A3 - Systems and methods for the production of differentiated cells - Google Patents

Systems and methods for the production of differentiated cells Download PDF

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Publication number
WO2007027226A3
WO2007027226A3 PCT/US2006/016228 US2006016228W WO2007027226A3 WO 2007027226 A3 WO2007027226 A3 WO 2007027226A3 US 2006016228 W US2006016228 W US 2006016228W WO 2007027226 A3 WO2007027226 A3 WO 2007027226A3
Authority
WO
WIPO (PCT)
Prior art keywords
cell
present
methods
undifferentiated
cells
Prior art date
Application number
PCT/US2006/016228
Other languages
French (fr)
Other versions
WO2007027226A2 (en
Inventor
Krishnendu Roy
Sabia Taqvi
Original Assignee
Univ Texas
Krishnendu Roy
Sabia Taqvi
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Texas, Krishnendu Roy, Sabia Taqvi filed Critical Univ Texas
Publication of WO2007027226A2 publication Critical patent/WO2007027226A2/en
Publication of WO2007027226A3 publication Critical patent/WO2007027226A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0636—T lymphocytes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/125—Stem cell factor [SCF], c-kit ligand [KL]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/23—Interleukins [IL]
    • C12N2501/2307—Interleukin-7 (IL-7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40—Regulators of development
    • C12N2501/42—Notch; Delta; Jagged; Serrate
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
    • C12N2502/1394—Bone marrow stromal cells; whole marrow
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2531/00—Microcarriers

Abstract

The present invention generally relates to methods for the ex vivo expansion of undifferentiated cells. More specifically, the present invention relates to systems and methods of differentiating undifferentiated cells into a desired linage by providing to an undifferentiated cell stromal cell conditioned medium and a differentiation inducing ligand. The system of the present invention may be used to expand any undifferentiated cell that requires cell-derived soluble factors and cell-contact dependent signals. The present invention does not rely on transfected stromal cells as the signaling entity for the creation of differentiated cells.
PCT/US2006/016228 2005-04-28 2006-04-28 Systems and methods for the production of differentiated cells WO2007027226A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US67580305P 2005-04-28 2005-04-28
US60/675,803 2005-04-28

Publications (2)

Publication Number Publication Date
WO2007027226A2 WO2007027226A2 (en) 2007-03-08
WO2007027226A3 true WO2007027226A3 (en) 2007-04-26

Family

ID=37809320

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2006/016228 WO2007027226A2 (en) 2005-04-28 2006-04-28 Systems and methods for the production of differentiated cells

Country Status (1)

Country Link
WO (1) WO2007027226A2 (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3298131B1 (en) 2015-05-20 2023-04-26 The Regents of The University of California Method for generating human dendritic cells for immunotherapy
CN105039257A (en) * 2015-07-24 2015-11-11 深圳爱生再生医学科技有限公司 In-vitro expansion cultivation method of hematopoietic stem cells
JP6983771B2 (en) 2015-10-30 2021-12-17 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニアThe Regents Of The University Of California A method for producing T cells from stem cells and an immunotherapeutic method using the T cells.
CA3091158A1 (en) * 2018-02-14 2019-08-22 Sunnybrook Research Institute Method for generating cells of the t cell lineage
WO2019226812A1 (en) * 2018-05-22 2019-11-28 The Charles Stark Draper Laboratory, Inc. System and method to generate progenitor cells
WO2020023807A1 (en) * 2018-07-26 2020-01-30 The Regents Of The University Of California Treatment of vascular occlusion by activation of notch signaling
JP2021534785A (en) * 2018-08-28 2021-12-16 フレッド ハッチンソン キャンサー リサーチ センター Methods and Compositions for Adoptive T Cell Therapy Using Induced Notch Signaling

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003087159A2 (en) * 2002-04-05 2003-10-23 Lorantis Limited Modulators of the notch signalling pathway and uses thereof in medical treatment

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003087159A2 (en) * 2002-04-05 2003-10-23 Lorantis Limited Modulators of the notch signalling pathway and uses thereof in medical treatment

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
DE SMEDT M. ET AL.: "Human bone marrow CD34+ progenitor cells mature to T cells on OP9-DL1 stromal cell line without thymus microenvironment", BLOOD CELLS, MOLECULES, AND DISEASES, vol. 33, 2004, pages 227 - 232, XP004620472 *
KARANU F.N. ET AL.: "Human homologous of Delta-1 and Delta-4 function as mitogenic regulators of primitive human hematopoietic cells", BLOOD, vol. 97, no. 7, 2001, pages 1960 - 1967, XP003010490 *
LA MOTTE-MOHS R.N. ET AL.: "Induction of T-cell development from human cord blood hematopoietic stem cells by Delta-like 1 in vitro", BLOOD, vol. 105, no. 4, 15 February 2005 (2005-02-15), pages 1431 - 1439, XP009058726 *
LEHAR S.M. ET AL.: "Notch ligands Delta1 and Jagged1 transmit distinct signals to T-cell precursors", BLOOD, vol. 105, no. 4, 15 February 2005 (2005-02-15), pages 1440 - 1447, XP003010489 *
SCHMITT T.M. ET AL.: "Induction of T Cell Development from Hematopoietic progenitor Cells by Delta-like-1 in Vitro", IMMUNITY, vol. 17, 2002, pages 749 - 756, XP002338760 *
VARNUM-FINNEY B. ET AL.: "The Notch Ligand, Jagged-1, Influences the Development of Primitive Hematopoietic Precursor Cells", BLOOD, vol. 91, no. 11, 1998, pages 4084 - 4091, XP002916386 *

Also Published As

Publication number Publication date
WO2007027226A2 (en) 2007-03-08

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