WO2007024825A2 - Separation par immunoaffinite et compositions et procedes d'analyse - Google Patents

Separation par immunoaffinite et compositions et procedes d'analyse Download PDF

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Publication number
WO2007024825A2
WO2007024825A2 PCT/US2006/032712 US2006032712W WO2007024825A2 WO 2007024825 A2 WO2007024825 A2 WO 2007024825A2 US 2006032712 W US2006032712 W US 2006032712W WO 2007024825 A2 WO2007024825 A2 WO 2007024825A2
Authority
WO
WIPO (PCT)
Prior art keywords
mixture
protein
fraction
antibodies
igy
Prior art date
Application number
PCT/US2006/032712
Other languages
English (en)
Other versions
WO2007024825A3 (fr
Inventor
Xiangming Fang
Lei Huang
Wei-Wei Zhang
Original Assignee
Genway Biotech, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genway Biotech, Inc. filed Critical Genway Biotech, Inc.
Publication of WO2007024825A2 publication Critical patent/WO2007024825A2/fr
Publication of WO2007024825A3 publication Critical patent/WO2007024825A3/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals

Definitions

  • the IgY antibodies can be individually immobilized to a solid support in a single or mixture format, or immobilized to a solid support as a mixture in certain ratio of the antibodies.
  • the immobilized IgY antibodies being placed in various types of holders or devices and applicable for the processes described above, are useful for immunoaffmity capture, separation, enrichment, purification, and detection of a desired protein target in a complex mixture.
  • Example 3 Preparation of IgY Microbead Mixture with Different Approaches.

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Medicinal Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Biotechnology (AREA)
  • Cell Biology (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Peptides Or Proteins (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

La présente invention concerne des compositions et procédés destinés à la séparation par immunoaffinité de cibles provenant de mélanges à des fins d'enrichissement, d'identification, de quantification et d'analyse. L'invention concerne plus particulièrement des anticorps IgY aviaires couplés à des supports solides ainsi que des procédés d'utilisation correspondants. L'invention concerne aussi des systèmes et procédés pour fractionner ou enrichir un mélange de matériaux biologiques dans une plate-forme ou un système automatisé multiplex et à débit élevé.
PCT/US2006/032712 2005-08-26 2006-08-23 Separation par immunoaffinite et compositions et procedes d'analyse WO2007024825A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US71200205P 2005-08-26 2005-08-26
US60/712,002 2005-08-26

Publications (2)

Publication Number Publication Date
WO2007024825A2 true WO2007024825A2 (fr) 2007-03-01
WO2007024825A3 WO2007024825A3 (fr) 2008-01-10

Family

ID=37772270

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2006/032712 WO2007024825A2 (fr) 2005-08-26 2006-08-23 Separation par immunoaffinite et compositions et procedes d'analyse

Country Status (2)

Country Link
US (1) US20070048795A1 (fr)
WO (1) WO2007024825A2 (fr)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008058325A1 (fr) * 2006-11-13 2008-05-22 Healthlinx Limited Compositions à immunoaffinité destinées à une immunodéplétion des protéines
US9000134B2 (en) 2007-05-11 2015-04-07 Wallace B. Haigh Reagent and kit for early diagnosis of kidney disease

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WO2006072140A1 (fr) * 2005-01-06 2006-07-13 Macquarie University Detection de molecules
MX2009013470A (es) * 2007-06-08 2010-01-27 Quest Diagnostics Invest Inc Analisis de lipoproteinas por movilidad diferencial de particulas cargadas.
US8247235B2 (en) 2007-06-08 2012-08-21 Quest Diagnostics Investments Incorporated Lipoprotein analysis by differential charged-particle mobility
US20080302666A1 (en) * 2007-06-08 2008-12-11 Benner W Henry Apparatus for differential charged-particle mobility
US9354200B1 (en) 2008-08-07 2016-05-31 Quest Diagnostics Investments Incorporated Detection apparatus for differential-charged particle mobility analyzer
US9182411B2 (en) * 2009-07-14 2015-11-10 Colen Innovations, L.L.C. Methods and apparatus for the detection and differentiation of non-sialated proteins from sialated proteins in a fluid sample
US9250211B2 (en) 2010-12-30 2016-02-02 Quest Diagnostics Investments Incorporated Magnetic separation of lipoproteins using dextran sulfate
CN107179378B (zh) * 2016-03-11 2018-08-21 中国科学院大连化学物理研究所 一种同时分离代谢组和脂质组组份的液相色谱分析方法及其应用

Citations (4)

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WO2002055654A2 (fr) * 2001-01-09 2002-07-18 Large Scale Proteomics Corp Technique d'immunosoustraction destinee a la preparation d'un echantillon pour electrophorese en gel en deux dimensions (2-dge)
WO2004019009A1 (fr) * 2002-08-23 2004-03-04 Royal Women's Hospital Depletion de proteines de plasma
AU780853B2 (en) * 1999-11-16 2005-04-21 Igeneon Krebs-Immuntherapie Forschungs- Und Entwicklungs-Ag Use of anti-idiotypical antibodies as vaccines against cancer

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2366455A1 (fr) * 1999-03-26 2000-10-05 Bioserv Ag Immuno-adsorbants pour le traitement de la septicemie
AU780853B2 (en) * 1999-11-16 2005-04-21 Igeneon Krebs-Immuntherapie Forschungs- Und Entwicklungs-Ag Use of anti-idiotypical antibodies as vaccines against cancer
WO2002055654A2 (fr) * 2001-01-09 2002-07-18 Large Scale Proteomics Corp Technique d'immunosoustraction destinee a la preparation d'un echantillon pour electrophorese en gel en deux dimensions (2-dge)
WO2004019009A1 (fr) * 2002-08-23 2004-03-04 Royal Women's Hospital Depletion de proteines de plasma

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AKITA E. ET AL.: 'Production and purification of Fab fragments from chicken egg yolk immunoglobulin Y (IgY)' J. IMMUNOLOGICAL METHODS vol. 162, no. 2, 1993, pages 155 - 164 *
FANG X. ET AL.: 'Affinity Separation: divide and conquer the proteome' DRUG DISCOVERY TODAY: TECHNOLOGIES vol. 1, 2004, pages 411 - 420 *
HINERFELD D.D. ET AL.: 'Serum/Plasma depletion with chicken immunoglobulin Y antibodies for proteomic analysis from multiple mammalian species' J. BIOMOLECULAR TECHNIQUES: JBT vol. 15, no. 3, September 2004, pages 184 - 190 *
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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008058325A1 (fr) * 2006-11-13 2008-05-22 Healthlinx Limited Compositions à immunoaffinité destinées à une immunodéplétion des protéines
US9000134B2 (en) 2007-05-11 2015-04-07 Wallace B. Haigh Reagent and kit for early diagnosis of kidney disease

Also Published As

Publication number Publication date
US20070048795A1 (en) 2007-03-01
WO2007024825A3 (fr) 2008-01-10

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