WO2007016221A3 - Hyaluronan synthases and methods of making and using same - Google Patents

Hyaluronan synthases and methods of making and using same Download PDF

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Publication number
WO2007016221A3
WO2007016221A3 PCT/US2006/029145 US2006029145W WO2007016221A3 WO 2007016221 A3 WO2007016221 A3 WO 2007016221A3 US 2006029145 W US2006029145 W US 2006029145W WO 2007016221 A3 WO2007016221 A3 WO 2007016221A3
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WO
WIPO (PCT)
Prior art keywords
hyaluronan synthase
functionally active
hyaluronan
enzymatic activity
altered enzymatic
Prior art date
Application number
PCT/US2006/029145
Other languages
French (fr)
Other versions
WO2007016221A2 (en
Inventor
Paul L. Deangelis
Paul H. Weigel
Phillip Pummill
Original Assignee
The Board Of Regents Of University Of Oklahoma
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by The Board Of Regents Of University Of Oklahoma filed Critical The Board Of Regents Of University Of Oklahoma
Publication of WO2007016221A2 publication Critical patent/WO2007016221A2/en
Publication of WO2007016221A3 publication Critical patent/WO2007016221A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

A functionally active hyaluronan synthase having at least one modified amino acid residue therein as compared to a corresponding functionally active native hyaluronan synthase such that the functionally active hyaluronan synthase has an altered enzymatic activity as compared to the corresponding functionally active native hyaluronan synthase is disclosed. The altered enzymatic activity may result in increased or decreased activity when compared to the corresponding native hyaluronan synthase, or the altered enzymatic activity may result in production of hyaluronan having an average molecular mass that is greater than or less than an average molecular mass of hyaluronan produced by the corresponding native hyaluronan synthase. Methods of producing hyaluronic acid utilizing a recombinant host cell having an expression construct encoding the functionally active hyaluronan synthase with altered enzymatic activity are also disclosed.
PCT/US2006/029145 2005-07-29 2006-07-28 Hyaluronan synthases and methods of making and using same WO2007016221A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US70400305P 2005-07-29 2005-07-29
US60/704,003 2005-07-29

Publications (2)

Publication Number Publication Date
WO2007016221A2 WO2007016221A2 (en) 2007-02-08
WO2007016221A3 true WO2007016221A3 (en) 2010-06-03

Family

ID=37709162

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2006/029145 WO2007016221A2 (en) 2005-07-29 2006-07-28 Hyaluronan synthases and methods of making and using same

Country Status (1)

Country Link
WO (1) WO2007016221A2 (en)

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030092118A1 (en) * 1997-10-31 2003-05-15 Deangelis Paul L. Hyaluronan synthase genes and expression thereof in bacillus hosts
US20030235893A1 (en) * 1998-10-26 2003-12-25 Weigel Paul H. Hyaluronan synthases and methods of making and using same

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030092118A1 (en) * 1997-10-31 2003-05-15 Deangelis Paul L. Hyaluronan synthase genes and expression thereof in bacillus hosts
US20030235893A1 (en) * 1998-10-26 2003-12-25 Weigel Paul H. Hyaluronan synthases and methods of making and using same

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
TLAPAK-SIMMONS ET AL.: "Kinetic Characterization of the Recombinant Hyaluronan Synthases from Streptococcus pyogenes and Streptococcus equisimilis.", JOUMAL OF BIOLOGICAL CHEMISTRY, vol. 274, no. 7, 12 February 1999 (1999-02-12), pages 4246 - 4253, XP055036909, DOI: doi:10.1074/jbc.274.7.4246 *

Also Published As

Publication number Publication date
WO2007016221A2 (en) 2007-02-08

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