WO2007014150A3 - Method of administering liposomes containing oligonucleotides - Google Patents

Method of administering liposomes containing oligonucleotides Download PDF

Info

Publication number
WO2007014150A3
WO2007014150A3 PCT/US2006/028658 US2006028658W WO2007014150A3 WO 2007014150 A3 WO2007014150 A3 WO 2007014150A3 US 2006028658 W US2006028658 W US 2006028658W WO 2007014150 A3 WO2007014150 A3 WO 2007014150A3
Authority
WO
WIPO (PCT)
Prior art keywords
cationic
liposomal composition
bis
effective amount
cationic liposomal
Prior art date
Application number
PCT/US2006/028658
Other languages
French (fr)
Other versions
WO2007014150A2 (en
Inventor
Jeffrey Sherman
Kristen Parker
Imran Ahmad
Original Assignee
Neopharm Inc
Jeffrey Sherman
Kristen Parker
Imran Ahmad
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Neopharm Inc, Jeffrey Sherman, Kristen Parker, Imran Ahmad filed Critical Neopharm Inc
Publication of WO2007014150A2 publication Critical patent/WO2007014150A2/en
Publication of WO2007014150A3 publication Critical patent/WO2007014150A3/en

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • A61K48/0008—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy characterised by an aspect of the 'non-active' part of the composition delivered, e.g. wherein such 'non-active' part is not delivered simultaneously with the 'active' part of the composition
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/10—Dispersions; Emulsions
    • A61K9/127—Liposomes
    • A61K9/1271—Non-conventional liposomes, e.g. PEGylated liposomes, liposomes coated with polymers
    • A61K9/1272—Non-conventional liposomes, e.g. PEGylated liposomes, liposomes coated with polymers with substantial amounts of non-phosphatidyl, i.e. non-acylglycerophosphate, surfactants as bilayer-forming substances, e.g. cationic lipids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C12N15/1135—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against oncogenes or tumor suppressor genes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/11—Antisense

Abstract

The invention provides a method of treating a cellular proliferative disease, a method of inhibiting the growth of neoplastic cells and a method of reducing the expression of a raf sequence by administering an effective amount of a cationic liposomal composition. The cationic liposomal composition comprises c-raf Antisense Oligonucleotide (c-rafAON), a cationic cardiolipin analogue and, desirably, another lipid species. The preferred cationic cardiolipin analogue is l,3-Bis-(l,2-bis-tetradecyloxy-propyl-3- dimethylethoxyammoniumbromide)-propane-2-ol (PCL-2). The preferred lipids include cholesterol, dioleoylphosphatidylethanolamine (DOPE), 1,2 Dioleoyl-sn-glycero-3- phosphocholine (DOPC), alpha tocopheryl acid succinate and phosphatidylcholine. The effective amount of the cationic liposomal composition is about 60 mg/m2.
PCT/US2006/028658 2005-07-22 2006-07-24 Method of administering liposomes containing oligonucleotides WO2007014150A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US70177505P 2005-07-22 2005-07-22
US60/701,775 2005-07-22

Publications (2)

Publication Number Publication Date
WO2007014150A2 WO2007014150A2 (en) 2007-02-01
WO2007014150A3 true WO2007014150A3 (en) 2007-05-24

Family

ID=37683879

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2006/028658 WO2007014150A2 (en) 2005-07-22 2006-07-24 Method of administering liposomes containing oligonucleotides

Country Status (1)

Country Link
WO (1) WO2007014150A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8815599B2 (en) 2004-06-01 2014-08-26 Pronai Therapeutics, Inc. Methods and compositions for the inhibition of gene expression
US8367628B2 (en) 2005-12-01 2013-02-05 Pronai Therapeutics, Inc. Amphoteric liposome formulation
WO2008103431A2 (en) * 2007-02-23 2008-08-28 Pronai Therapeutics, Inc. Dnai - liposomes

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5563255A (en) * 1994-05-31 1996-10-08 Isis Pharmaceuticals, Inc. Antisense oligonucleotide modulation of raf gene expression
WO1998043095A1 (en) * 1997-03-21 1998-10-01 Georgetown University Liposomes containing oligonucleotides
WO2004035523A1 (en) * 2002-10-16 2004-04-29 Neopharm, Inc. Cationic cardiolipin analogs and use thereof

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5563255A (en) * 1994-05-31 1996-10-08 Isis Pharmaceuticals, Inc. Antisense oligonucleotide modulation of raf gene expression
WO1998043095A1 (en) * 1997-03-21 1998-10-01 Georgetown University Liposomes containing oligonucleotides
US6126965A (en) * 1997-03-21 2000-10-03 Georgetown University School Of Medicine Liposomes containing oligonucleotides
WO2004035523A1 (en) * 2002-10-16 2004-04-29 Neopharm, Inc. Cationic cardiolipin analogs and use thereof

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
SIMONZADEH ET AL.: "Determination of cationic cardiolipin in liposomal formulations by a robust HPLC method", 227TH AMERICAN CHEMICAL SOCIETY NATIONAL MEETING, 28 March 2004 (2004-03-28) - 1 April 2004 (2004-04-01), pages ABSTR. NO. 148 *

Also Published As

Publication number Publication date
WO2007014150A2 (en) 2007-02-01

Similar Documents

Publication Publication Date Title
JP6685338B2 (en) Amine cationic lipids and uses thereof
US9387262B2 (en) Coated lipid complexes and their use
US20230212578A1 (en) Compositions and methods for treating hypertriglyceridemia
KR102205278B1 (en) Process for formulating an anionic agent
US10945956B2 (en) Biodegradable compound, lipid particles, composition and kit comprising lipid particles
EP2873732A1 (en) Rnai pharmaceutical composition capable of suppressing expression of kras gene
WO2007014150A3 (en) Method of administering liposomes containing oligonucleotides
WO2005067632A3 (en) Lipid compositions and use thereof
AU2008230379B2 (en) Prompt nucleic acid delivery carrier composition
WO2015186770A1 (en) Ckap5-gene-silencing rnai pharmaceutical composition
Wong et al. A lipid-based delivery system for antisense oligonucleotides derived from a hydrophobic complex
US20130090369A1 (en) Transfection sheets and methods of use
PL355231A1 (en) Cationic dosper virosomes
Sternberg et al. Steric stabilization of cationic liposome-DNA complexes: influence on morphology and transfection activity

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application
NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 06788296

Country of ref document: EP

Kind code of ref document: A2