WO2007014076A3 - Methods of producing modified assembly lines and related compositions - Google Patents

Methods of producing modified assembly lines and related compositions Download PDF

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Publication number
WO2007014076A3
WO2007014076A3 PCT/US2006/028487 US2006028487W WO2007014076A3 WO 2007014076 A3 WO2007014076 A3 WO 2007014076A3 US 2006028487 W US2006028487 W US 2006028487W WO 2007014076 A3 WO2007014076 A3 WO 2007014076A3
Authority
WO
WIPO (PCT)
Prior art keywords
modified assembly
assembly lines
methods
related compositions
producing modified
Prior art date
Application number
PCT/US2006/028487
Other languages
French (fr)
Other versions
WO2007014076A2 (en
Inventor
Christopher T Walsh
Michael A Fischbach
Jonathan R Lai
David R Liu
Zhe Zhou
Original Assignee
Harvard College
Christopher T Walsh
Michael A Fischbach
Jonathan R Lai
David R Liu
Zhe Zhou
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Harvard College, Christopher T Walsh, Michael A Fischbach, Jonathan R Lai, David R Liu, Zhe Zhou filed Critical Harvard College
Priority to US11/989,257 priority Critical patent/US20100048422A1/en
Publication of WO2007014076A2 publication Critical patent/WO2007014076A2/en
Publication of WO2007014076A3 publication Critical patent/WO2007014076A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

The present invention provides a method producing a modified assembly line, such as those that produce non-ribosomal peptides and polyketides. The modified assembly lines of the invention can be used to produce novel compounds with therapeutic activities. The invention also provides organisms containing modified assembly lines and libraries of modified assembly lines.
PCT/US2006/028487 2005-07-21 2006-07-21 Methods of producing modified assembly lines and related compositions WO2007014076A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
US11/989,257 US20100048422A1 (en) 2005-07-21 2006-07-21 Methods of Producing Modified Assembly Lines and Related Compositions

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US70180705P 2005-07-21 2005-07-21
US60/701,807 2005-07-21

Publications (2)

Publication Number Publication Date
WO2007014076A2 WO2007014076A2 (en) 2007-02-01
WO2007014076A3 true WO2007014076A3 (en) 2007-05-24

Family

ID=37683846

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2006/028487 WO2007014076A2 (en) 2005-07-21 2006-07-21 Methods of producing modified assembly lines and related compositions

Country Status (2)

Country Link
US (1) US20100048422A1 (en)
WO (1) WO2007014076A2 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013191678A1 (en) 2012-06-18 2013-12-27 Board Of Trustees Of Michigan State University Biosynthesis of paclitaxel intermediate
WO2016196940A1 (en) * 2015-06-05 2016-12-08 Massachusetts Institute Of Technology Compressed pathways for nonribosomal molecular biosynthesis
WO2017020983A1 (en) 2015-08-05 2017-02-09 Merck Patent Gmbh Artificial non-ribosomal peptide synthetases
EP3414323A1 (en) 2016-02-08 2018-12-19 Johann Wolfgang Goethe-Universität Frankfurt am Main Artificial non-ribosomal peptide synthases and their use
EP3511445A1 (en) 2018-01-15 2019-07-17 Johann Wolfgang Goethe-Universität Frankfurt System for the assembly and modification of non-ribosomal peptide synthases
WO2024038117A1 (en) 2022-08-16 2024-02-22 Johann Wolfgang Goethe-Universität Frankfurt Method and means for engineering non-ribosomal peptides

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
DEL VECCHIO ET AL.: "Active-site residue, domain and module swaps in molecular polyketide synthases", J. IND. MICROBIOL. BIOTECHNOL., vol. 30, August 2003 (2003-08-01), pages 489 - 494, XP003013070 *
MCDANIEL ET AL.: "Multiple genetic modifications of the erythromycin polyketide synthase to produce a library of novel "unnatural" natural products", PROC. NATL. ACAD. SCI. USA, vol. 96, March 1999 (1999-03-01), pages 1846 - 1851, XP002143433 *

Also Published As

Publication number Publication date
US20100048422A1 (en) 2010-02-25
WO2007014076A2 (en) 2007-02-01

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