WO2007012377A3 - Methods for the electromanipulation of cells in order to modify the membrane permeability of said cells with the aid of lipophilic ions as a medium additive - Google Patents

Methods for the electromanipulation of cells in order to modify the membrane permeability of said cells with the aid of lipophilic ions as a medium additive Download PDF

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Publication number
WO2007012377A3
WO2007012377A3 PCT/EP2006/006388 EP2006006388W WO2007012377A3 WO 2007012377 A3 WO2007012377 A3 WO 2007012377A3 EP 2006006388 W EP2006006388 W EP 2006006388W WO 2007012377 A3 WO2007012377 A3 WO 2007012377A3
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WO
WIPO (PCT)
Prior art keywords
cells
methods
modify
aid
order
Prior art date
Application number
PCT/EP2006/006388
Other languages
German (de)
French (fr)
Other versions
WO2007012377A2 (en
Inventor
Dirk Zimmermann
Vladimir Soukhoroukov
Ernst Bamberg
Ulrich Zimmermann
Original Assignee
Eppendorf Ag
Dirk Zimmermann
Vladimir Soukhoroukov
Ernst Bamberg
Ulrich Zimmermann
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Eppendorf Ag, Dirk Zimmermann, Vladimir Soukhoroukov, Ernst Bamberg, Ulrich Zimmermann filed Critical Eppendorf Ag
Priority to AU2006274294A priority Critical patent/AU2006274294A1/en
Priority to EP06762319A priority patent/EP1907541A2/en
Priority to JP2008523158A priority patent/JP2009506756A/en
Publication of WO2007012377A2 publication Critical patent/WO2007012377A2/en
Publication of WO2007012377A3 publication Critical patent/WO2007012377A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N13/00—Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
    • C07F11/00—Compounds containing elements of Groups 6 or 16 of the Periodic Table
    • C07F11/005—Compounds containing elements of Groups 6 or 16 of the Periodic Table compounds without a metal-carbon linkage
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
    • C07F13/00—Compounds containing elements of Groups 7 or 17 of the Periodic Table
    • C07F13/005—Compounds without a metal-carbon linkage
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
    • C07F5/00—Compounds containing elements of Groups 3 or 13 of the Periodic Table
    • C07F5/06—Aluminium compounds
    • C07F5/061—Aluminium compounds with C-aluminium linkage
    • C07F5/066—Aluminium compounds with C-aluminium linkage compounds with Al linked to an element other than Al, C, H or halogen (this includes Al-cyanide linkage)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Toxicology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Thiazole And Isothizaole Compounds (AREA)
  • Heterocyclic Carbon Compounds Containing A Hetero Ring Having Nitrogen And Oxygen As The Only Ring Hetero Atoms (AREA)

Abstract

The invention relates to methods for the treatment of cells with electric fields in order to modify the membrane permeability of said cells with the aid of lipophilic ions as a medium additive. The invention more particularly relates to cell electroporation and electrofusion methods using lipophilic ions as a medium additive.
PCT/EP2006/006388 2005-07-28 2006-06-30 Methods for the electromanipulation of cells in order to modify the membrane permeability of said cells with the aid of lipophilic ions as a medium additive WO2007012377A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
AU2006274294A AU2006274294A1 (en) 2005-07-28 2006-06-30 Methods for the electromanipulation of cells in order to modify the membrane permeability of said cells with the aid of lipophilic ions as a medium additive
EP06762319A EP1907541A2 (en) 2005-07-28 2006-06-30 Methods for the electromanipulation of cells in order to modify the membrane permeability of said cells with the aid of lipophilic ions as a medium additive
JP2008523158A JP2009506756A (en) 2005-07-28 2006-06-30 Electrical manipulation of cells to alter cell membrane permeability using lipophilic ions as media additives

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE102005035456.4 2005-07-28
DE200510035456 DE102005035456A1 (en) 2005-07-28 2005-07-28 A method of electromanipulating cells to alter the membrane permeability of these cells using lipophilic ions as a medium supplement

Publications (2)

Publication Number Publication Date
WO2007012377A2 WO2007012377A2 (en) 2007-02-01
WO2007012377A3 true WO2007012377A3 (en) 2007-04-12

Family

ID=36940028

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2006/006388 WO2007012377A2 (en) 2005-07-28 2006-06-30 Methods for the electromanipulation of cells in order to modify the membrane permeability of said cells with the aid of lipophilic ions as a medium additive

Country Status (6)

Country Link
EP (1) EP1907541A2 (en)
JP (1) JP2009506756A (en)
CN (1) CN101278048A (en)
AU (1) AU2006274294A1 (en)
DE (1) DE102005035456A1 (en)
WO (1) WO2007012377A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107841462B (en) * 2017-10-11 2021-07-16 山东第一医科大学(山东省医学科学院) Construction and use method of electrochemical controllable bacteria adhesion interface

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000022147A1 (en) * 1998-10-09 2000-04-20 Stratagene Improved method for more efficient electroporation
WO2001066694A2 (en) * 2000-03-06 2001-09-13 Eppendorf Ag Method for transferring material in a cell system
WO2002086134A2 (en) * 2001-04-23 2002-10-31 Amaxa Gmbh Buffer solution for electroporation and a method comprising the use of the same

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000022147A1 (en) * 1998-10-09 2000-04-20 Stratagene Improved method for more efficient electroporation
WO2001066694A2 (en) * 2000-03-06 2001-09-13 Eppendorf Ag Method for transferring material in a cell system
WO2002086134A2 (en) * 2001-04-23 2002-10-31 Amaxa Gmbh Buffer solution for electroporation and a method comprising the use of the same

Non-Patent Citations (11)

* Cited by examiner, † Cited by third party
Title
ASALI, KHALIL J. ET AL: "Reactivity of tungsten(0) and molybdenum(0) pentacarbonyl thiobenzoate anions: thiobenzoate as a cis-CO labilizing ligand", TRANSITION METAL CHEMISTRY (DORDRECHT, NETHERLANDS) , 28(2), 193-198 CODEN: TMCHDN; ISSN: 0340-4285, 2003, XP002412288 *
DATABASE CA [online] CHEMICAL ABSTRACTS SERVICE, COLUMBUS, OHIO, US; RAUBENHEIMER, HELGARD G. ET AL: "Pentacarbonyl(thione) Complexes of Chromium and Tungsten Prepared by Unusual Methods", XP002412292, retrieved from STN Database accession no. 129:75515 *
DATABASE CA [online] CHEMICAL ABSTRACTS SERVICE, COLUMBUS, OHIO, US; SZYMANSKA-BUZAR, T.: "Tungsten carbonyl complexes as olefin metathesis catalysts", XP002412291, retrieved from STN Database accession no. 116:6103 *
DAVIDSON, JACK L. ET AL: "Reversible Coordination of alkali metals to sulfur-based organometallic ligands [M(SC6F5)4(cp)]- (M = Mo or W, cp = .eta.5-C5H5)", JOURNAL OF THE CHEMICAL SOCIETY, DALTON TRANSACTIONS: INORGANIC CHEMISTRY (1972-1999) , (16), 2423-9 CODEN: JCDTBI; ISSN: 0300-9246, 1994, XP002412285 *
IVANOVA, SVETLANA M. ET AL: "Relative Lewis basicities of six Al(ORF)4- superweak anions and the structures of LiAl{OCH(CF3)2}4 and [1-Et-3-Me-1,3-C3H3N2][Li{Al{OCH(CF3)2} 4}2]", CHEMISTRY--A EUROPEAN JOURNAL , 7(2), 503-510 CODEN: CEUJED; ISSN: 0947-6539, 2001, XP002412286 *
JOURNAL OF CHEMICAL RESEARCH, SYNOPSES , (5), 250-251 CODEN: JRPSDC; ISSN: 0308-2342, 1998 *
JOURNAL OF MOLECULAR CATALYSIS , 68(2), 177-86 CODEN: JMCADS; ISSN: 0304-5102, 1991 *
KÜRSCHNER M ET AL: "Interaction of lipophilic ions with the plasma membrane of mammalian cells studies by electrorotation.", BIOPHYSICAL JOURNAL. JUN 1998, vol. 74, no. 6, June 1998 (1998-06-01), pages 3031 - 3043, XP002398095, ISSN: 0006-3495 *
PIETSCH, JOERG ET AL: "Koordinationschemie funktioneller Phosphine: Carbonyl- und Carbonyl(nitrosyl)wolfram-Derivate von 2-Diphenylphosphinanilin und 2-Diphenylphosphinobenzoesaüre", JOURNAL OF ORGANOMETALLIC CHEMISTRY , 472(1-2), 55-70 CODEN: JORCAI; ISSN: 0022-328X, 1994, XP002412287 *
SUKHORUKOV V L ET AL: "Surviving high-intensity field pulses: strategies for improving robustness and performance of electrotransfection and electrofusion", JOURNAL OF MEMBRANE BIOLOGY, SPRINGER, NEW YORK, NY, US, vol. 206, no. 3, August 2005 (2005-08-01), pages 187 - 201, XP002392462, ISSN: 0022-2631 *
ZHUANG, BOTAO ET AL: "Reactivity of the metal(0) dithiocarbamato carbonyl complex [M(CO)4(S2CNEt2)]- (M = Mo, W): synthesis, structure and cyclic voltammetry of the tungsten(0) dithiocarbamato carbonyl complex and a new mixed-valence dinuclear tungsten-sulfur compound [Et4N]2[(OC)4WS2WS2]", INORGANICA CHIMICA ACTA , 177(2), 239-46 CODEN: ICHAA3; ISSN: 0020-1693, 1990, XP002412284 *

Also Published As

Publication number Publication date
AU2006274294A1 (en) 2007-02-01
CN101278048A (en) 2008-10-01
DE102005035456A1 (en) 2007-03-08
EP1907541A2 (en) 2008-04-09
JP2009506756A (en) 2009-02-19
WO2007012377A2 (en) 2007-02-01

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