WO2007009036A3 - Apparatus and methods for preparing tissue grafts - Google Patents

Apparatus and methods for preparing tissue grafts Download PDF

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Publication number
WO2007009036A3
WO2007009036A3 PCT/US2006/027191 US2006027191W WO2007009036A3 WO 2007009036 A3 WO2007009036 A3 WO 2007009036A3 US 2006027191 W US2006027191 W US 2006027191W WO 2007009036 A3 WO2007009036 A3 WO 2007009036A3
Authority
WO
WIPO (PCT)
Prior art keywords
grafts
tissue
methods
tissue grafts
biochamber
Prior art date
Application number
PCT/US2006/027191
Other languages
French (fr)
Other versions
WO2007009036A9 (en
WO2007009036A2 (en
Inventor
Stuart K Williams
Paul Kosnik
Chris England
Thomas F Cannon
Erik Vossman
Eugene Boland
Christian L Haller
Craig A Mauch
Original Assignee
Tissue Genesis Inc
Stuart K Williams
Paul Kosnik
Chris England
Thomas F Cannon
Erik Vossman
Eugene Boland
Christian L Haller
Craig A Mauch
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US11/314,281 external-priority patent/US8202725B2/en
Application filed by Tissue Genesis Inc, Stuart K Williams, Paul Kosnik, Chris England, Thomas F Cannon, Erik Vossman, Eugene Boland, Christian L Haller, Craig A Mauch filed Critical Tissue Genesis Inc
Priority to CA002615208A priority Critical patent/CA2615208A1/en
Priority to EP06787137A priority patent/EP1902128A4/en
Priority to AU2006268129A priority patent/AU2006268129A1/en
Priority to JP2008521605A priority patent/JP2009501562A/en
Publication of WO2007009036A2 publication Critical patent/WO2007009036A2/en
Publication of WO2007009036A9 publication Critical patent/WO2007009036A9/en
Publication of WO2007009036A3 publication Critical patent/WO2007009036A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0697—Artificial constructs associating cells of different lineages, e.g. tissue equivalents
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/08—Bioreactors or fermenters specially adapted for specific uses for producing artificial tissue or for ex-vivo cultivation of tissue
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/14—Scaffolds; Matrices
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/10—Perfusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M45/00—Means for pre-treatment of biological substances
    • C12M45/06—Means for pre-treatment of biological substances by chemical means or hydrolysis

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Sustainable Development (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Cell Biology (AREA)
  • Materials For Medical Uses (AREA)
  • Prostheses (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The invention provides automated apparatus and methods for manufacturing implantable tissue grafts in an operating room setting, preferably utilizing autologous adipose tissue cells for preparing vascular grafts. The automated apparatus includes media and tissue dissociating chemical reservoirs, filters, a cell separator and a perfusion flow loop through a biochamber which supports a graft substrate. The present invention further provides methods for using the tissue grafts and cell samples prepared by the devices described herein in a multitude of therapies including revascularization, regeneration and reconstruction of tissues and organs, as well as treatment and prevention of diseases. The invention also provides methods biochamber configurations for applying a sustained low pressure gradient across a permeable scaffold material using a media containing cells to be deposited on the scaffold for the production of tissue grafts, including, but not limited to, vascular grafts.
PCT/US2006/027191 2005-07-12 2006-07-12 Apparatus and methods for preparing tissue grafts WO2007009036A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
CA002615208A CA2615208A1 (en) 2005-07-12 2006-07-12 Apparatus and methods for preparing tissue grafts
EP06787137A EP1902128A4 (en) 2005-07-12 2006-07-12 Apparatus and methods for preparing tissue grafts
AU2006268129A AU2006268129A1 (en) 2005-07-12 2006-07-12 Apparatus and methods for preparing tissue grafts
JP2008521605A JP2009501562A (en) 2005-07-12 2006-07-12 Apparatus and method for pretreatment of tissue graft

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US69795405P 2005-07-12 2005-07-12
US60/697,954 2005-07-12
US11/314,281 2005-12-22
US11/314,281 US8202725B2 (en) 2004-12-23 2005-12-22 Cell sodding method and apparatus

Publications (3)

Publication Number Publication Date
WO2007009036A2 WO2007009036A2 (en) 2007-01-18
WO2007009036A9 WO2007009036A9 (en) 2007-03-15
WO2007009036A3 true WO2007009036A3 (en) 2007-05-31

Family

ID=37637966

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2006/027191 WO2007009036A2 (en) 2005-07-12 2006-07-12 Apparatus and methods for preparing tissue grafts

Country Status (5)

Country Link
EP (1) EP1902128A4 (en)
JP (1) JP2009501562A (en)
AU (1) AU2006268129A1 (en)
CA (1) CA2615208A1 (en)
WO (1) WO2007009036A2 (en)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9144583B2 (en) * 2002-03-29 2015-09-29 Tissue Genesis, Inc. Cell separation apparatus and methods of use
US11174458B2 (en) 2007-04-23 2021-11-16 Koligo Therapeutics, Inc. Cell separation apparatus and methods of use
WO2009073724A1 (en) 2007-12-04 2009-06-11 Ingeneron, Inc. Apparatus and methods for cell isolation
US8865199B2 (en) * 2008-11-17 2014-10-21 Ingeneron, Inc. Biomatrix composition and methods of biomatrix seeding
US9173975B2 (en) 2009-04-24 2015-11-03 Ingeneron, Inc. Reparative cell delivery via hyaluronic acid vehicles
EP2464392A4 (en) * 2009-08-12 2013-04-10 Univ Duke Cell coated implantable device
AU2011258295B2 (en) 2010-05-25 2015-01-29 Cook Biotech Incorporated Methods, substrates, and systems useful for cell seeding of medical grafts
DE102011080218B4 (en) 2010-10-20 2014-11-20 Human Med Ag Method and apparatus for separating adult stem cells from adipose tissue
WO2013089808A1 (en) * 2011-12-14 2013-06-20 Edmund Burke Decellularized small particle tissue
WO2013158283A1 (en) * 2012-04-18 2013-10-24 The General Hospital Corporation Bioartificial filtration organ
EP4389872A2 (en) * 2015-10-09 2024-06-26 DEKA Products Limited Partnership Fluid pumping and bioreactor system
US11299705B2 (en) 2016-11-07 2022-04-12 Deka Products Limited Partnership System and method for creating tissue
US11464808B2 (en) 2017-04-28 2022-10-11 University Of Louisville Research Foundation Tissue constructs including pancreas derived microvessel fragments and related methods
JP7308525B2 (en) 2017-12-11 2023-07-14 学校法人東京女子医科大学 Tubular tissue preparation device, tubular tissue preparation kit, and tubular tissue preparation method

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5753267A (en) * 1995-02-10 1998-05-19 Purdue Research Foundation Method for enhancing functional properties of submucosal tissue graft constructs
US5792603A (en) * 1995-04-27 1998-08-11 Advanced Tissue Sciences, Inc. Apparatus and method for sterilizing, seeding, culturing, storing, shipping and testing tissue, synthetic or native, vascular grafts
US5928945A (en) * 1996-11-20 1999-07-27 Advanced Tissue Sciences, Inc. Application of shear flow stress to chondrocytes or chondrocyte stem cells to produce cartilage
US6416995B1 (en) * 1999-11-22 2002-07-09 Bio Science Consultants, L.L.C. Bioreactor mediated recellularization of natural and tissue engineered vascular grafts
US6479064B1 (en) * 1999-12-29 2002-11-12 Children's Medical Center Corporation Culturing different cell populations on a decellularized natural biostructure for organ reconstruction
US6734018B2 (en) * 1999-06-07 2004-05-11 Lifenet Process for decellularizing soft-tissue engineered medical implants, and decellularized soft-tissue medical implants produced

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1500417A (en) * 1974-11-07 1978-02-08 Merck & Co Inc Apparatus for producing cell suspensions from tissue and a process for extracting cells from tissue
US5409833A (en) * 1993-07-01 1995-04-25 Baxter International Inc. Microvessel cell isolation apparatus
WO2003053346A2 (en) * 2001-12-07 2003-07-03 Macropore Biosurgery, Inc. Systems and methods for treating patients with processed lipoaspirate cells

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5753267A (en) * 1995-02-10 1998-05-19 Purdue Research Foundation Method for enhancing functional properties of submucosal tissue graft constructs
US5792603A (en) * 1995-04-27 1998-08-11 Advanced Tissue Sciences, Inc. Apparatus and method for sterilizing, seeding, culturing, storing, shipping and testing tissue, synthetic or native, vascular grafts
US5928945A (en) * 1996-11-20 1999-07-27 Advanced Tissue Sciences, Inc. Application of shear flow stress to chondrocytes or chondrocyte stem cells to produce cartilage
US6734018B2 (en) * 1999-06-07 2004-05-11 Lifenet Process for decellularizing soft-tissue engineered medical implants, and decellularized soft-tissue medical implants produced
US6416995B1 (en) * 1999-11-22 2002-07-09 Bio Science Consultants, L.L.C. Bioreactor mediated recellularization of natural and tissue engineered vascular grafts
US6479064B1 (en) * 1999-12-29 2002-11-12 Children's Medical Center Corporation Culturing different cell populations on a decellularized natural biostructure for organ reconstruction

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See also references of EP1902128A4 *

Also Published As

Publication number Publication date
AU2006268129A1 (en) 2007-01-18
WO2007009036A9 (en) 2007-03-15
EP1902128A4 (en) 2009-02-25
EP1902128A2 (en) 2008-03-26
CA2615208A1 (en) 2007-01-18
JP2009501562A (en) 2009-01-22
WO2007009036A2 (en) 2007-01-18

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