WO2007002204A2 - Pyrosequencing methods and related compostions - Google Patents
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- WO2007002204A2 WO2007002204A2 PCT/US2006/024157 US2006024157W WO2007002204A2 WO 2007002204 A2 WO2007002204 A2 WO 2007002204A2 US 2006024157 W US2006024157 W US 2006024157W WO 2007002204 A2 WO2007002204 A2 WO 2007002204A2
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Definitions
- Pyrosequencing is a method based on the detection of the pyrophosphate group that is generated when a nucleotide is incorporated in a DNA polymerase reaction [1] .
- Each of the four deoxynucleotides (dNTPs) is added sequentially to the DNA template to be sequenced with a cocktail of enzymes and substrates in addition to the usual polymerase reaction components. If the added nucleotide is complementary with the first available base on the template, the nucleotide will be incorporated and a pyrophosphate will be released. The released pyrophosphate is converted to ATP by sulfurylase, and this ATP is the substrate for a luciferase, e.g.
- dATP greatly interferes with the luciferase detection system, which is deficient in the detection of dATP.
- This invention provides a method for determining the nucleotide sequence of a single-stranded DNA comprising performing the following steps for each nucleic acid residue of the DNA whose identity is to be determined:
- step (a) contacting the DNA under DNA polymerization-permitting conditions with (i) a 3' -O-blocked dNTP selected from the group consisting of 3' -O-blocked dATP, 3'-O- blocked dCTP, 3' -O-blocked dGTP, arid 3' -O-blocked dTTP, and (ii) 9 0 N DNA polymerase (exo-) A4851/Y409V or another DNA polymerase able to incorporate 3'-O- blocked dNTPs; (b) (i) determining whether pyrophosphate is generated as a result of step (a), whereby (1) pyrophosphate generation indicates that polymerization has occurred and the identity of the nucleic acid residue in the DNA is that which is complementary to the 3' -O-blocked dNTP used in part (i) of step (a), and (2) the absence of pyrophosphate generation indicates that the identity of such nucleic acid residue is not that which is
- step (ii) if pyrophosphate is not generated, repeating step (a) once, twice or three times as necessary, wherein in each repetition a 3' -O-blocked dNTP is used which is different from any 3' -O-blocked dNTP already used, and determining, after each repetition of step (a) , whether pyrophosphate is generated, such generation indicating that polymerization has occurred and the identity of the nucleic acid residue in the DNA is that which is complementary to the 3' -O-blocked dNTP used in part (i) of the repeated step (a) ; and (c) removing from the 3'-0-blocked dNTP polymerized in step (a) or (b) , whichever is applicable, the moiety blocking the 3'-0 atom of the dNTP, with the proviso that such removing step is optional in the event that there remains no further nucleic acid residue of the DNA whose identity is to be determined.
- Figure 1 Schematic of pyrosequencing in solution. Reactants not shown are APS, which with PPi is converted to ATP and SO 4 2" by ATP-sulfurylase .
- luciferase acts on ATP, luciferin and O 2 to give AMP, PPi, oxyluciferin, CO 2 and light, and apyrase converts ATP and dNTP to AMP, dNMP and 2Pi.
- Figure 2 3' -O-Allyl-dNTP (A, C, G, T), instead of dNTP, is used in the single base extension on a solid surface.
- Allyl-dNTPs are added iteratively.
- the complementary base is incorporated, the pyrophosphate that is produced from the reaction can be detected by its reaction with the light- generating luciferase system or a receptor- indicator (R-I) coordination compound via the release of the fluorescent indicator molecule.
- R-I receptor- indicator
- the extended primers can be deallylated, washed and reused in the next round.
- the use of an allyl-group solves inherent problems of traditional pyrosequencing.
- Figures 3A-3C Schematic representation and step-by-step MALDI-TOF MS results for the deallylation of an allyl-modified oligonucleotide (SEQ ID NO:1) and the use of the deallylated DNA product as a primer in a polymerase extension reaction.
- SEQ ID NO:1 allyl-modified oligonucleotide
- B Peak at m/z 5831 corresponding to the above DNA product without the allyl group, obtained after 30 sees of incubation with the Na 2 PdCl 4 catalyst and the TPPTS (P(PhSO 3 Na) 3 ) ligand at 70 0 C.
- C Peak at m/z 6535 corresponding to the extension of the deallylated DNA product by Biotin-ddGTP using Thermo Sequenase DNA Polymerase.
- Figure 4 Synthesis of a 3' -0-allyl-m.odified oligonucleotide .
- Figures 5A & 5B MALDI-TOF MS spectra showing the incorporation of 3' -O-allyl-modified dTTP into a growing DNA strand by 9°N Polymerase (exo-)
- Figures 6A & 6B (A) Receptor (R) : Zn 2+ -dipicolylamine (Zn2+DPA) ; (B) Indicator (I): fluorescent molecule (coumarin-derived indicator) . See [5] .
- Figures 7A & 7B When R is titrated into I, the fluorescence intensity of I will decrease: (A) Zn 2+ DPA is titrated into (lO ⁇ M) I; (B) PPi is added to R-I coordination compound.
- Figure 8 Ronaghi's real-time pyrosequencing .
- Figure 9 Improved real-time pyrosequencing method.
- Figure 10 Structures of four reversibly-blocked nucleotides .
- Figure 11 Mass spectrometry traces showing incorporation of four different reversibly- blocked allyl-dNTPs into a growing DNA strand in the solution phase.
- Figure 12 ⁇ Polymerase extension reaction with 3'-O- allyl-dGTP-allyl-biodipy-FL-510 as a reversible terminator of SEQ ID NO : 2.
- Figure 13 Experimental results of pyrosequencing a DNA template (SEQ ID NO: 3) in solution with allyl- dGTP and comparison with ⁇ regular' unblocked nucleotides . The results indicate that allyl-dGTP is a good terminator in solution phase, and the incorporation Signal can be easily detected.
- Figure 14 An experimental scheme of a method employing allyl-dGTP for pyrosequencing with attachment of the primer (SEQ ID NO: 4 and SEQ ID NO: 5) to a solid surface/bead using an NHS ester.
- Figure 15 Comparison of pyrosequencing using ⁇ regular' dNTPs and pyrosequencing using reversibly-blocked dNTPS (SEQ ID N0:3).
- Figure 16 Pyrosequencing data using reversible terminators on sepharose bead immobilized looped primer-DNA (SEQ ID NO:3).
- Figure 17 Light production by luciferase in the presence of dATP and in the presence of allyl- dATP, demonstrating that allyl-dATP is not a luciferase substrate.
- Figure 18 Technique of immobilizing double-stranded DNA (SEQ ID NO : 6 (top strand) and SEQ ID NO: 7
- FIG. 19 Pyrosequencing on sepharose bead-immobilized DNA (SEQ ID NO : 6 (top strand) and SEQ ID NO : 7
- TPPTS tri sodium salt of tri (m-sulfophenyl) -phosphine
- Nucleic acid shall mean any nucleic acid molecule, including, without limitation, DNA, RNA and hybrids thereof.
- the nucleic acid bases that form nucleic acid molecules can be the bases A, C, G, T and U, as well as derivatives thereof. Derivatives of these bases are well known in the art, and are exemplified in PCR Systems, Reagents and Consumables (Perkin Elmer Catalogue 1996-1997, Roche Molecular Systems, Inc., Branchburg, New Jersey, USA) .
- Disclosed here is a method that solves the problems of homopolymeric regions and dATP interference by using 3'-O- allyl-nucleotides as reversible terminators in pyrosequencing using either a new PPi detection system (Chemosensing Ensemble) , or the traditional luciferase detection technique.
- this invention provides a method for determining the nucleotide sequence of a single-stranded DNA comprising performing the following steps for each nucleic acid residue of the DNA whose identity is to be determined:
- step (a) contacting the DNA under DNA polymerization-permitting conditions with (i) a 3'-0-blocked dNTP selected from the group consisting of 3'-0-blocked dATP, 3'-O- blocked 'dCTP, 3'-0-blocked dGTP, and 3'-0 ⁇ blocked dTTP, and (ii) 9°N DNA polymerase (exo-) A4851/Y409V or other DNA polymerase; (b) (i) determining whether pyrophosphate is generated as a result of step (a) , whereby (1) pyrophosphate generation indicates that polymerization has occurred and the identity of the nucleic acid residue in the DNA is that which is complementary to the 3'-0-blocked dNTP used in part (i) of step (a), and (2) the absence of pyrophosphate generation indicates that the identity of such nucleic acid residue is not that which is complementary to such 3'-0-blocked dNTP, and (ii) if pyro
- step (a) determines, after each repetition of step (a) , whether pyrophosphate is generated, such generation indicating that polymerization has occurred and the identity of the nucleic acid residue in the DNA is that which is complementary to the 3' -O-blocked dNTP used in part (i) of the repeated step (a) ; and (c) removing from the 3' -O-blocked dNTP polymerized in step (a) or (b) , whichever is applicable, the moiety blocking the 3'-0 atom of the dNTP, with the proviso that such removing step is optional in the event that there remains no further nucleic acid residue of the DNA whose identity is to be determined.
- the identity of a nucleic acid residue in the DNA being sequenced is that which is complementary to the 3'-O- blocked dNTP incorporated, i.e. such identity is determined by the well-established complementary base-pairing rules. For example, if a 3' -O-blocked dATP is incorporated, then the corresponding nucleic acid residue in the DNA being sequenced is a thymine. If a 3' -O-blocked dGTP is incorporated, then the corresponding nucleic acid residue in the DNA being sequenced is a cytosine, and so forth with the understanding that adenine and thymine are complements of each other, and guanine and cytosine are complements of each other. In addition, uridine is a complement of adenine .
- a 3' -O-blocked deoxynucleotide is a deoxynucleotide having attached to the 3' oxygen of its sugar component a chemical group, for example an allyl group, that precludes further polymerization from the 3' oxygen until that blocking group is removed.
- This invention further provides the instant method, wherein determining whether pyrophosphate generated in step (b) (i) is performed by detecting light generated by a luciferase- based reaction.
- the luciferase is firefly luciferase.
- the luciferase- based reaction comprises contacting the pyrophosphate with a sulfurylase under conditions permitting the generation of ATP from the pyrophosphate, and contacting the ATP so generated with a luciferase under conditions permitting the generation of light by the luciferase in the presence of ATP.
- a luciferase-based reaction includes, for example, the reaction of luciferin and ATP in the presence of luciferase and O 2 , whereby oxyluciferin, AMP, PPi, CO 2 , and light are produced.
- the light produced can be measured by any standard photometry technique including, but not limited to, photomultiplier, video, CCD, CCCD, and the naked eye.
- the moiety blocking the 3'-0 atom of the dNTP is an allyl moiety and the single-stranded DNA is immobilized to a solid substrate.
- determining whether pyrophosphate is generated in step (b) (i) is performed by detecting dissociation of a coumarin-derived indicator from a complex between the indicator and a bis-Zn 2+ -dipicolylamine coordination compound, wherein the coumarin-derived indicator has the following structure:
- the moiety blocking the 3'-0 atom of the dNTP is an allyl moiety and the single-stranded DNA is immobilized to a solid substrate.
- the moiety blocking the 3'-0 atom of the dNTP is an allyl moiety or a methoxymethyl moiety.
- the moiety is an allyl moiety.
- the DNA is immobilized on a solid substrate.
- the DNA is bound to the solid substrate via an azido linkage, an alkynyl linkage, a 1,3-dipolar cycloaddition linkage, or biotin-streptavidin interaction.
- the solid substrate can be, for example, in the form of a chip, a bead, a well, a capillary tube, or a slide.
- the solid substrate can be gold, quartz, silica, or plastic.
- the solid substrate is porous.
- Single-stranded DNA can be immobilized on a solid surface, for example a glass surface, by a 1,3-dipolar cycloaddition reaction in the presence of a Cu(I) catalyst.
- the DNA is labeled with an azido group at the 5' end, while the glass surface is modified by an alkynyl group.
- the DNA is covalently attached to the surface via a stable 1, 2, 3-triazole linkage.
- the positions of the azido and the alkynyl functional groups are interchangeable.
- the resulting 1, 2 , 3-triazoles are stable at aqueous conditions and high temperature.
- the moiety blocking the 3'-0 atom of the dNTP is an allyl moiety and removing it is performed using Na 2 PdC ⁇ and
- This invention also provides a compound comprising a dNTP having bound to its 3' oxygen an allyl or methoxymethyl moiety.
- the moiety is an allyl moiety.
- the moiety is a methoxymethyl moiety.
- the dNTP is dATP, dCTP, dGTP, or dTTP.
- the instant compound is a 3'-O-allyl dNTP, and specifically 3'- 0-allyl dATP, 3'-O-allyl dCTP, 3'-O-allyl dGTP, 3'-0-allyl dUTP or 3'-O-allyl dTTP.
- allyl derivatives include, without limitation, analogs or homologs thereof, or haloallyls such as iodoallyl, chloroallyl and fluoroallyl which perform as blocking moieties.
- methoxymethyl derivatives include, without limitation, analogs or homologs thereof which perform as blocking moieties.
- This invention also provides a process for producing a 3'- 0-allyl dNTP comprising:
- step (a) sequentially contacting a dimethoxytrityl (DMTr) 3' protected nucleoside triphosphate with (i) 3-bromo propene, NaOH and benzene, and (ii) a suitable solvent; and (b) sequentially contacting the product of step (a) with (i) POCl 3 / (MeO) 3 P (O) , (ii) tributylammonium pyrophosphate, and (iii) TEAB/NH 4 0H, so as to produce the 3'-O-allyl dNTP.
- DMTr dimethoxytrityl
- the suitable solvent of step (a) (ii) is 3% THF/CHC1 3 .
- the concentration of TEAB in step (b) (iii) is about 0.1M.
- this invention provides a kit for use in sequencing a single-stranded DNA comprising:
- the instant kit further comprises (i) a 9°N DNA polymerase (exo-) A4851/Y409V, (ii) reagents permitting DNA polymerization, (iii) reagents permitting pyrophosphate detection using a luciferase-based reaction,
- the 3' -O-allyl-modified dNTP has one of the following structures:
- Fig. 2 The general scheme of the improved pyrosequencing method is shown in Fig. 2.
- 3' -O-allyl-dNTPs (A, C, G, T), instead of dNTPs, are used in the single base extension on a solid surface.
- Four allyl-dNTPs are added iteratively.
- the complementary base is incorporated, the pyrophosphate that is produced from the reaction can be detected by its reaction with the light-generating luciferase system or a receptor-indicator (R-I) coordination compound via the release of the fluorescent indicator molecule.
- the R-I compound has negligible or no fluorescence, and the released/displaced indicator is detectably fluorescent.
- the extended primers can be deallylated, washed and reused in the next round.
- the use of an allyl group solves inherent problems of traditional pyrosequencing.
- AGAGGATCCAACCGAGAC-T (allyl) -3' ) (SEQ ID NO: 8) was established using MALDI-TOF mass spectrometry.
- the mass peak at m/z 5871 corresponds to the mass of the purified oligonucleotide bearing the allyl group.
- the deallylation reaction on this oligonucleotide was carried out using the Na 2 PdCWTPPTS system.
- Fig. 3B shows near complete deallylation with a DNA/catalyst/ligand ratio of 1/50/400 in a reaction time of 30 sees, as shown by the mass peak at m/z 5831.
- the next step was to prove that the deallylated product could be used in a primer extension reaction and that deallylation did not hinder the continuation of the polymerase reaction.
- a single base extension reaction using the deallylated product as a primer was performed with a synthetic template and a Biotin-ddGTP nucleotide terminator complementary to the base immediately adjacent to the priming site on the template.
- the extension product was isolated using solid phase capture purification and analyzed using MALDI-TOF MS [4] .
- the mass spectrum in Fig. 3C shows a clear peak at m/z 6535 corresponding to the extension product proving that the deallylated product can be successfully used as a primer in a polymerase reaction.
- a nucleotide analogue 3' -allyloxythymidine triphosphate (3'-O-allyl- dTTP) was synthesized (Fig. 4) and its incorporation ability was tested using a mutant form of 9°N DNA
- O-allyl-dATP and 3' -O-allyl-dCTP can be similarly prepared according to the scheme set forth in Fig. 4.
- the 3' -O-allyl-thymidine triphosphate was used in a primer extension reaction to demonstrate its ability to be incorporated into a growing DNA strand by DNA Polymerase.
- the extension was performed using a 15- ⁇ l reaction mixture consisting of 50 pmol of an 18-mer primer (5' -AGA-GGA-TCC- AAC-CGA-GAC-3' ) (SEQ ID NO : 9 ) , 100 pmol of single-stranded 60-mer DNA template (5'-GTG-TAC-ATC-AAC-ATC-ACC-TAC-CAC- CAT-GTC-AGT-CTC-GGT-TGG-ATC-CTC-TAT-TGT-GTC-CGG-S') (SEQ ID NO: 10) corresponding to a portion of exon 7 of the p53 gene (200 pmol of 3' -O-allyl-thymidine triphosphate), IX Thermopol reaction buffer (New England Biolabs) and 15 U of 9 0 N DNA polymerase (exo-) A485L/Y409V.
- an 18-mer primer 5' -AGA-GGA-TCC- A
- Fig. 5(A) shows a single mass peak at m/z 5526 corresponding to the unextended primer.
- Fig. 5(B) shows a single peak at m/z 5869 corresponding to the primer extended by a single base 3'-O- allyl-thymidine triphosphate.
- the primers can be immobilized on a solid surface.
- One common method is to use paramagnetic beads which are coated with streptavidin. Primers which are labeled with biotin can be attached to the beads because of the biotin-streptavidin attraction.
- a recently developed DNA immobilization method using click chemistry, [6] hereby- incorporated by reference, can be used in the pyrosequencing method disclosed here. With the addition of template, ally-dNTP and polymerase, the extension can take place on the beads .
- pyrophosphate is capable of displacing a fluorescent coumarin-derived indicator (I) from a bis-Zn 2+ -dipicolylamine (Zn 2+ DPA) coordination compound (R) . See Fig. 6. With an increase of the Receptor (R) amount, the non-fluorescent R-I coordination compound is formed. When the proportion of R is 50%, the fluorescence reaches its lowest, indicating a 1:1 stoichiometry .
- pyrophosphate Once pyrophosphate is added to the solution, it can replace the Indicator (fluorescence molecule) from the R-I coordination compound. Therefore, the fluorescence molecule is released/displaced, and the fluorescence intensity of the solution will increase (Fig. 7A and B) .
- Ronaghi proposed a real time pyrosequencing method in solution [1] .
- four enzymes are needed. Among them, sulfurylase is used to transfer PPi to ATP; then luciferase is used to generate light that indicates PPi has been generated. In the next step apyrase is used to degrade
- ATP and excess dNTP in the reaction then the process goes to the next round.
- apyrase activity is decreased in later cycles, which is due to the accumulation of intermediate products (such as deoxynucleoside diphosphate, or dNDP) and eventually undegraded dNTP. Because of this limitation, this method can determine the sequence of only about 100 bases at most. See Fig. 8.
- the method disclosed here using the R-I complex can greatly improve the real-time pyrosequencing in the Ronaghi method. (Fig. 9) .
- the R-I complex is used to detect PPi. PPi is converted to PPi-R, while the released indicator I can be transferred to the R-I complex by adding R without removing the components from the solution.
- the excess dNTP in each cycle is degraded by apyrase. Because there will be no ATP produced in the detection steps, apyrase now primarily degrades dNTP and is more efficient in its action. Accordingly, more bases can be determined.
- Another advantage of this method is that only two kinds of enzymes are used here rather than four, and the detection step will not adversely affect the other steps.
- this improved method cannot detect the bases in homopolymeric regions either, and so 3'-0 allyl dNTPs are employed to circumvent this problem.
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Abstract
This invention provides methods for pyrosequencing and compositions comprising 3'-O- modified deoxynucleoside triphosphates .
Description
PYROSEQUENCING METHODS AND RELATED COMPOSITIONS
This application claims benefit of U.S. Provisional Application No. 60/692,816, filed June 21, 2005, the contents of which are hereby incorporated by reference. The invention disclosed herein was made with government support under a grant from the Center for Excellence in Genomic Science Grant No. P50 HG002806. Accordingly, the U.S. Government has certain rights in this invention.
Throughout this application, various publications are referenced in parentheses by number. Full citations for these references may be found at the end of the specification immediately preceding the claims. The disclosures of these publications in their entireties are hereby incorporated by reference into this application to more fully describe the state of the art to which this invention pertains.
Background of the Invention
Pyrosequencing is a method based on the detection of the pyrophosphate group that is generated when a nucleotide is incorporated in a DNA polymerase reaction [1] . Each of the four deoxynucleotides (dNTPs) is added sequentially to the DNA template to be sequenced with a cocktail of enzymes and substrates in addition to the usual polymerase reaction components. If the added nucleotide is complementary with the first available base on the template, the nucleotide will be incorporated and a pyrophosphate will be released. The released pyrophosphate is converted to ATP by
sulfurylase, and this ATP is the substrate for a luciferase, e.g. firefly luciferase, which reaction produces visible light. If the added nucleotide is not incorporated, no light will be produced and the nucleotide will simply be degraded by the enzyme apyrase. This pyrosequencing technique, schematized in Fig. 1, has been applied to single nucleotide polymorphism (SNP) detection and other applications [2] .
There are, however, inherent difficulties in the traditional pyrosequencing method for determining the number of incorporated nucleotides in homopolymeric regions
(e.g. a string of several T's in a row) of the template.
Moreover, dATP greatly interferes with the luciferase detection system, which is deficient in the detection of dATP.
Summary of the Invention
This invention provides a method for determining the nucleotide sequence of a single-stranded DNA comprising performing the following steps for each nucleic acid residue of the DNA whose identity is to be determined:
(a) contacting the DNA under DNA polymerization-permitting conditions with (i) a 3' -O-blocked dNTP selected from the group consisting of 3' -O-blocked dATP, 3'-O- blocked dCTP, 3' -O-blocked dGTP, arid 3' -O-blocked dTTP, and (ii) 90N DNA polymerase (exo-) A4851/Y409V or another DNA polymerase able to incorporate 3'-O- blocked dNTPs; (b) (i) determining whether pyrophosphate is generated as a result of step (a), whereby (1) pyrophosphate generation indicates that polymerization has occurred and the identity of the nucleic acid residue in the DNA is that which is complementary to the 3' -O-blocked dNTP used in part (i) of step (a), and (2) the absence of pyrophosphate generation indicates that the identity of such nucleic acid residue is not that which is complementary to such 3' -O-blocked dNTP, and
(ii) if pyrophosphate is not generated, repeating step (a) once, twice or three times as necessary, wherein in each repetition a 3' -O-blocked dNTP is used which is different from any 3' -O-blocked dNTP already used, and determining, after each repetition of step (a) , whether pyrophosphate is generated, such generation indicating that polymerization has occurred and the identity of the nucleic acid residue in the DNA is that which is complementary to the 3' -O-blocked dNTP used in part (i) of the repeated step (a) ; and
(c) removing from the 3'-0-blocked dNTP polymerized in step (a) or (b) , whichever is applicable, the moiety blocking the 3'-0 atom of the dNTP, with the proviso that such removing step is optional in the event that there remains no further nucleic acid residue of the DNA whose identity is to be determined.
Brief Description of the Figures
Figure 1: Schematic of pyrosequencing in solution. Reactants not shown are APS, which with PPi is converted to ATP and SO4 2" by ATP-sulfurylase . In addition luciferase acts on ATP, luciferin and O2 to give AMP, PPi, oxyluciferin, CO2 and light, and apyrase converts ATP and dNTP to AMP, dNMP and 2Pi.
Figure 2: 3' -O-Allyl-dNTP (A, C, G, T), instead of dNTP, is used in the single base extension on a solid surface. Four Allyl-dNTPs are added iteratively. Once the complementary base is incorporated, the pyrophosphate that is produced from the reaction can be detected by its reaction with the light- generating luciferase system or a receptor- indicator (R-I) coordination compound via the release of the fluorescent indicator molecule. Then the extended primers can be deallylated, washed and reused in the next round. The use of an allyl-group solves inherent problems of traditional pyrosequencing.
Figures 3A-3C: Schematic representation and step-by-step MALDI-TOF MS results for the deallylation of an allyl-modified oligonucleotide (SEQ ID NO:1) and the use of the deallylated DNA product as a primer in a polymerase extension reaction. (A) Peak at m/z 5871 corresponding to the HPLC- purified 3'-allyloxy 19-mer oligonucleotide. (B) Peak at m/z 5831 corresponding to the above DNA product without the allyl group, obtained after
30 sees of incubation with the Na2PdCl4 catalyst and the TPPTS (P(PhSO3Na)3) ligand at 70 0C. (C) Peak at m/z 6535 corresponding to the extension of the deallylated DNA product by Biotin-ddGTP using Thermo Sequenase DNA Polymerase.
Figure 4 : Synthesis of a 3' -0-allyl-m.odified oligonucleotide .
Figures 5A & 5B: MALDI-TOF MS spectra showing the incorporation of 3' -O-allyl-modified dTTP into a growing DNA strand by 9°N Polymerase (exo-)
A485L/Y409V; (A) unextended primer at m/z 5526;
(B) primer extended with 3' -O-allyl-dTTP at m/z 5869.
Figures 6A & 6B: (A) Receptor (R) : Zn2+-dipicolylamine (Zn2+DPA) ; (B) Indicator (I): fluorescent molecule (coumarin-derived indicator) . See [5] .
Figures 7A & 7B: When R is titrated into I, the fluorescence intensity of I will decrease: (A) Zn2+DPA is titrated into (lOμM) I; (B) PPi is added to R-I coordination compound.
Figure 8 : Ronaghi's real-time pyrosequencing .
Figure 9 : Improved real-time pyrosequencing method.
Figure 10: Structures of four reversibly-blocked nucleotides .
Figure 11: Mass spectrometry traces showing incorporation of four different reversibly- blocked allyl-dNTPs into a growing DNA strand in the solution phase.
Figure 12 : ■ Polymerase extension reaction with 3'-O- allyl-dGTP-allyl-biodipy-FL-510 as a reversible terminator of SEQ ID NO : 2.
Figure 13: Experimental results of pyrosequencing a DNA template (SEQ ID NO: 3) in solution with allyl- dGTP and comparison with Λregular' unblocked nucleotides . The results indicate that allyl-dGTP is a good terminator in solution phase, and the incorporation Signal can be easily detected.
Figure 14: An experimental scheme of a method employing allyl-dGTP for pyrosequencing with attachment of the primer (SEQ ID NO: 4 and SEQ ID NO: 5) to a solid surface/bead using an NHS ester.
Figure 15 : Comparison of pyrosequencing using λregular' dNTPs and pyrosequencing using reversibly-blocked dNTPS (SEQ ID N0:3).
Figure 16: Pyrosequencing data using reversible terminators on sepharose bead immobilized looped primer-DNA (SEQ ID NO:3).
Figure 17 : Light production by luciferase in the presence of dATP and in the presence of allyl-
dATP, demonstrating that allyl-dATP is not a luciferase substrate.
Figure 18 : Technique of immobilizing double-stranded DNA (SEQ ID NO : 6 (top strand) and SEQ ID NO: 7
(lower strand) ) to a derivatized bead and pyrosequencing using "normal" nucleotides.
Figure 19: Pyrosequencing on sepharose bead-immobilized DNA (SEQ ID NO : 6 (top strand) and SEQ ID NO : 7
(lower strand)) using Allyl-dNTPs .
Detailed Description of the Invention
Definitions
As used herein, and unless stated otherwise, each of the following terms shall have the definition set forth below.
PPi - pyrophosphate dNTP - deoxynucleoside 5' -triphosphate - also known as a deoxynucleotide
APS - adenosine 5' -phosphosulfate
ATP - adenosine 5' -triphosphate dATP - deoxyadenosine 5' -triphosphate
THF - tetrahydrofuran TEAB - tetraethylammonium bromide
TPPTS - tri sodium salt of tri (m-sulfophenyl) -phosphine
"Nucleic acid" shall mean any nucleic acid molecule, including, without limitation, DNA, RNA and hybrids thereof. The nucleic acid bases that form nucleic acid molecules can be the bases A, C, G, T and U, as well as derivatives thereof. Derivatives of these bases are well known in the art, and are exemplified in PCR Systems, Reagents and Consumables (Perkin Elmer Catalogue 1996-1997, Roche Molecular Systems, Inc., Branchburg, New Jersey, USA) .
Embodiments of the Invention
Disclosed here is a method that solves the problems of homopolymeric regions and dATP interference by using 3'-O- allyl-nucleotides as reversible terminators in pyrosequencing using either a new PPi detection system
(Chemosensing Ensemble) , or the traditional luciferase detection technique.
Specifically, this invention provides a method for determining the nucleotide sequence of a single-stranded DNA comprising performing the following steps for each nucleic acid residue of the DNA whose identity is to be determined:
(a) contacting the DNA under DNA polymerization-permitting conditions with (i) a 3'-0-blocked dNTP selected from the group consisting of 3'-0-blocked dATP, 3'-O- blocked 'dCTP, 3'-0-blocked dGTP, and 3'-0~blocked dTTP, and (ii) 9°N DNA polymerase (exo-) A4851/Y409V or other DNA polymerase; (b) (i) determining whether pyrophosphate is generated as a result of step (a) , whereby (1) pyrophosphate generation indicates that polymerization has occurred and the identity of the nucleic acid residue in the DNA is that which is complementary to the 3'-0-blocked dNTP used in part (i) of step (a), and (2) the absence of pyrophosphate generation indicates that the identity of such nucleic acid residue is not that which is complementary to such 3'-0-blocked dNTP, and (ii) if pyrophosphate is not generated, repeating step (a) once, twice or three times as necessary (i.e. until pyrophosphate is generated) , wherein in each repetition a 3'-0-blocked dNTP is used which is different from any 3'-0-blocked dNTP already used, and determining, after each repetition of step (a) , whether pyrophosphate is generated, such generation indicating that polymerization has occurred and the identity of the nucleic acid residue in the DNA is
that which is complementary to the 3' -O-blocked dNTP used in part (i) of the repeated step (a) ; and (c) removing from the 3' -O-blocked dNTP polymerized in step (a) or (b) , whichever is applicable, the moiety blocking the 3'-0 atom of the dNTP, with the proviso that such removing step is optional in the event that there remains no further nucleic acid residue of the DNA whose identity is to be determined.
The identity of a nucleic acid residue in the DNA being sequenced is that which is complementary to the 3'-O- blocked dNTP incorporated, i.e. such identity is determined by the well-established complementary base-pairing rules. For example, if a 3' -O-blocked dATP is incorporated, then the corresponding nucleic acid residue in the DNA being sequenced is a thymine. If a 3' -O-blocked dGTP is incorporated, then the corresponding nucleic acid residue in the DNA being sequenced is a cytosine, and so forth with the understanding that adenine and thymine are complements of each other, and guanine and cytosine are complements of each other. In addition, uridine is a complement of adenine .
A 3' -O-blocked deoxynucleotide is a deoxynucleotide having attached to the 3' oxygen of its sugar component a chemical group, for example an allyl group, that precludes further polymerization from the 3' oxygen until that blocking group is removed.
This invention further provides the instant method, wherein determining whether pyrophosphate generated in step (b) (i) is performed by detecting light generated by a luciferase- based reaction. In one embodiment, the luciferase is
firefly luciferase. In another embodiment, the luciferase- based reaction comprises contacting the pyrophosphate with a sulfurylase under conditions permitting the generation of ATP from the pyrophosphate, and contacting the ATP so generated with a luciferase under conditions permitting the generation of light by the luciferase in the presence of ATP. A luciferase-based reaction includes, for example, the reaction of luciferin and ATP in the presence of luciferase and O2, whereby oxyluciferin, AMP, PPi, CO2, and light are produced. The light produced can be measured by any standard photometry technique including, but not limited to, photomultiplier, video, CCD, CCCD, and the naked eye.
In a preferred embodiment, the moiety blocking the 3'-0 atom of the dNTP is an allyl moiety and the single-stranded DNA is immobilized to a solid substrate.
In another embodiment, determining whether pyrophosphate is generated in step (b) (i) is performed by detecting dissociation of a coumarin-derived indicator from a complex between the indicator and a bis-Zn2+-dipicolylamine coordination compound, wherein the coumarin-derived indicator has the following structure:
and the bis-Zn2+-dipicolylamine coordination compound, when in association with the coumarin-derived indicator, has the following structure:
4 NO3 '
In the preferred embodiment, the moiety blocking the 3'-0 atom of the dNTP is an allyl moiety and the single-stranded DNA is immobilized to a solid substrate. In another embodiment the moiety blocking the 3'-0 atom of the dNTP is an allyl moiety or a methoxymethyl moiety. Preferably, the moiety is an allyl moiety.
In the preferred embodiment of the instant method, the DNA is immobilized on a solid substrate. In different embodiments, the DNA is bound to the solid substrate via an azido linkage, an alkynyl linkage, a 1,3-dipolar cycloaddition linkage, or biotin-streptavidin interaction. The solid substrate can be, for example, in the form of a chip, a bead, a well, a capillary tube, or a slide. Also, for example, the solid substrate can be gold, quartz, silica, or plastic. In one embodiment of this invention, the solid substrate is porous.
Single-stranded DNA can be immobilized on a solid surface, for example a glass surface, by a 1,3-dipolar cycloaddition reaction in the presence of a Cu(I) catalyst. The DNA is labeled with an azido group at the 5' end, while the glass surface is modified by an alkynyl group. After the 1,3-dipolar cycloaddition between the azido and the alkynyl group in the presence of a Cu(I) catalyst at room temperature, the DNA is covalently attached to the surface via a stable 1, 2, 3-triazole linkage. The positions of the
azido and the alkynyl functional groups are interchangeable. The resulting 1, 2 , 3-triazoles are stable at aqueous conditions and high temperature.
In the preferred embodiment of the instant methods, the moiety blocking the 3'-0 atom of the dNTP is an allyl moiety and removing it is performed using Na2PdC^ and
TPPTS.
This invention also provides a compound comprising a dNTP having bound to its 3' oxygen an allyl or methoxymethyl moiety. In the preferred embodiment, the moiety is an allyl moiety. In another embodiment, the moiety is a methoxymethyl moiety. In specific embodiments the dNTP is dATP, dCTP, dGTP, or dTTP. In a further embodiment, the instant compound is a 3'-O-allyl dNTP, and specifically 3'- 0-allyl dATP, 3'-O-allyl dCTP, 3'-O-allyl dGTP, 3'-0-allyl dUTP or 3'-O-allyl dTTP.
Examples of allyl derivatives include, without limitation, analogs or homologs thereof, or haloallyls such as iodoallyl, chloroallyl and fluoroallyl which perform as blocking moieties. Examples of methoxymethyl derivatives include, without limitation, analogs or homologs thereof which perform as blocking moieties.
This invention also provides a process for producing a 3'- 0-allyl dNTP comprising:
(a) sequentially contacting a dimethoxytrityl (DMTr) 3' protected nucleoside triphosphate with (i) 3-bromo propene, NaOH and benzene, and (ii) a suitable solvent; and
(b) sequentially contacting the product of step (a) with (i) POCl3/ (MeO)3P (O) , (ii) tributylammonium pyrophosphate, and (iii) TEAB/NH40H, so as to produce the 3'-O-allyl dNTP.
In one embodiment of the instant method, the suitable solvent of step (a) (ii) is 3% THF/CHC13. In another embodiment, the concentration of TEAB in step (b) (iii) is about 0.1M.
Finally, this invention provides a kit for use in sequencing a single-stranded DNA comprising:
(a) 3'-O-allyl dATP, 3'-O-allyl dCTP, 3'-0-allyl dTTP, and
3'-O-allyl dGTP, each in a separate compartment; and (b) instructions for use.
In various embodiments, the instant kit further comprises (i) a 9°N DNA polymerase (exo-) A4851/Y409V, (ii) reagents permitting DNA polymerization, (iii) reagents permitting pyrophosphate detection using a luciferase-based reaction,
(iv) reagents permitting pyrophosphate detection using a coumarin-derived indicator, and/or (v) reagents permitting removal of an allyl group from a 3'-0-allyl dNTP.
In differing embodiments, the 3' -O-allyl-modified dNTP has one of the following structures:
This invention will be better understood by reference to the Experimental Details which follow, but those skilled in the art will readily appreciate that the specific experiments detailed are only illustrative of the invention as described more fully in the claims which follow thereafter .
Experimental Details
The general scheme of the improved pyrosequencing method is shown in Fig. 2. 3' -O-allyl-dNTPs (A, C, G, T), instead of dNTPs, are used in the single base extension on a solid surface. Four allyl-dNTPs are added iteratively. Once the complementary base is incorporated, the pyrophosphate that is produced from the reaction can be detected by its reaction with the light-generating luciferase system or a receptor-indicator (R-I) coordination compound via the release of the fluorescent indicator molecule. In one case the R-I compound has negligible or no fluorescence, and the released/displaced indicator is detectably fluorescent. Then the extended primers can be deallylated, washed and reused in the next round. The use of an allyl group solves inherent problems of traditional pyrosequencing.
Synthesis and Deallylation of 3' -O-Allyl-dNTP and its Performance in Single Base Extension
A mild condition to remove a 3'-O-allyl group from DNA in aqueous solution using a catalyst system formed by Na2PdCl4 and a water-soluble ligand 3,3',3''- phosphinidynetris (benzenesulfonic acid) , trisodium salt
(TPPTS) [3] has been identified. Using this condition, the deallylation of the purified 19-mer oligonucleotide (5'-
AGAGGATCCAACCGAGAC-T (allyl) -3' ) (SEQ ID NO: 8) was established using MALDI-TOF mass spectrometry. In Fig. 3A, the mass peak at m/z 5871 corresponds to the mass of the purified oligonucleotide bearing the allyl group. The deallylation reaction on this oligonucleotide was carried out using the Na2PdCWTPPTS system. Fig. 3B shows near
complete deallylation with a DNA/catalyst/ligand ratio of 1/50/400 in a reaction time of 30 sees, as shown by the mass peak at m/z 5831.
The next step was to prove that the deallylated product could be used in a primer extension reaction and that deallylation did not hinder the continuation of the polymerase reaction. A single base extension reaction using the deallylated product as a primer was performed with a synthetic template and a Biotin-ddGTP nucleotide terminator complementary to the base immediately adjacent to the priming site on the template. The extension product was isolated using solid phase capture purification and analyzed using MALDI-TOF MS [4] . The mass spectrum in Fig. 3C shows a clear peak at m/z 6535 corresponding to the extension product proving that the deallylated product can be successfully used as a primer in a polymerase reaction.
These experiments established that Na2PdCl4 and TPPTS could be used to efficiently carry out deallylation on DNA in an aqueous environment without the need for an allyl scavenger or harsh conditions . A next step was to ensure that an allyl-modified nucleotide could be incorporated in a DNA
Polymerase reaction. For this purpose, a nucleotide analogue 3' -allyloxythymidine triphosphate (3'-O-allyl- dTTP) was synthesized (Fig. 4) and its incorporation ability was tested using a mutant form of 9°N DNA
Polymerase (exo-) bearing the mutations A485L and Y409V.
Results showed that this enzyme could incorporate 3'-O- allyl-dTTP in a polymerase reaction. 3' -O-allyl-dGTP, 3'-
O-allyl-dATP and 3' -O-allyl-dCTP can be similarly prepared according to the scheme set forth in Fig. 4.
The 3' -O-allyl-thymidine triphosphate was used in a primer extension reaction to demonstrate its ability to be incorporated into a growing DNA strand by DNA Polymerase. The extension was performed using a 15-μl reaction mixture consisting of 50 pmol of an 18-mer primer (5' -AGA-GGA-TCC- AAC-CGA-GAC-3' ) (SEQ ID NO : 9 ) , 100 pmol of single-stranded 60-mer DNA template (5'-GTG-TAC-ATC-AAC-ATC-ACC-TAC-CAC- CAT-GTC-AGT-CTC-GGT-TGG-ATC-CTC-TAT-TGT-GTC-CGG-S') (SEQ ID NO: 10) corresponding to a portion of exon 7 of the p53 gene (200 pmol of 3' -O-allyl-thymidine triphosphate), IX Thermopol reaction buffer (New England Biolabs) and 15 U of 90N DNA polymerase (exo-) A485L/Y409V. The extension reaction consisted of 15 cycles at 94 0C for 20 sec, 48 0C for 30 sec and 60 0C for 60 sec. The product was desalted using Zip Tip and analyzed using MALDI-TOF MS. The mass spectral data are shown in Fig. 5. Fig. 5(A) shows a single mass peak at m/z 5526 corresponding to the unextended primer. Fig. 5(B) shows a single peak at m/z 5869 corresponding to the primer extended by a single base 3'-O- allyl-thymidine triphosphate. These data confirm that the above 3'-allyl- modified nucleotide analogue can be efficiently incorporated by 9°N DNA polymerase (exo-) A485L/Y409V.
Single Base Extension on Solid Surface with 3' -Allyl-dNTP
(Click Chemistry)
In order to separate primers from the mixture after SBE and deallylation, the primers can be immobilized on a solid surface. One common method is to use paramagnetic beads which are coated with streptavidin. Primers which are labeled with biotin can be attached to the beads because of the biotin-streptavidin attraction. A recently developed
DNA immobilization method using click chemistry, [6] hereby- incorporated by reference, can be used in the pyrosequencing method disclosed here. With the addition of template, ally-dNTP and polymerase, the extension can take place on the beads .
A New PPi Detection System for use in Pyrosequencing (Chemosensing Ensemble)
Roger reported a fluorescence chemosensing system (Chemosensing Ensemble) which is described and shown to selectively detect pyrophosphate under physiological conditions [5] . Here, pyrophosphate is capable of displacing a fluorescent coumarin-derived indicator (I) from a bis-Zn2+-dipicolylamine (Zn2+DPA) coordination compound (R) . See Fig. 6. With an increase of the Receptor (R) amount, the non-fluorescent R-I coordination compound is formed. When the proportion of R is 50%, the fluorescence reaches its lowest, indicating a 1:1 stoichiometry . Once pyrophosphate is added to the solution, it can replace the Indicator (fluorescence molecule) from the R-I coordination compound. Therefore, the fluorescence molecule is released/displaced, and the fluorescence intensity of the solution will increase (Fig. 7A and B) .
Schematically :
PPi + R-I Complex ► PPi-R + I
(no fluorescence) (strong fluorescence)
Using 3' -allyl-dNTPs as reversible terminators overcomes the inherent problem that the pyrosequencing method otherwise has in accurately detecting the bases in homopolymeric regions, because each base via this invention
is extended one by one with high fidelity. Meanwhile, the newly designed PPi detection system is simple to use and is not affected by dATP. The paradigm of pyrosequencing can be useful in the presence of automatic sequencing machines where each step is repeated in cycles.
Improvement upon the Ronaghi Method
Ronaghi proposed a real time pyrosequencing method in solution [1] . In his method, four enzymes are needed. Among them, sulfurylase is used to transfer PPi to ATP; then luciferase is used to generate light that indicates PPi has been generated. In the next step apyrase is used to degrade
ATP and excess dNTP in the reaction; then the process goes to the next round. However, apyrase activity is decreased in later cycles, which is due to the accumulation of intermediate products (such as deoxynucleoside diphosphate, or dNDP) and eventually undegraded dNTP. Because of this limitation, this method can determine the sequence of only about 100 bases at most. See Fig. 8.
However, replacing dNTPs in Fig. 8 with the 3' -Allyl-dNTPs disclosed here, and then following the scheme in Fig. 8, permits one to unambiguously sequence the DNA using repeated cycles without the same degradation problems.
The method disclosed here using the R-I complex can greatly improve the real-time pyrosequencing in the Ronaghi method. (Fig. 9) . The R-I complex is used to detect PPi. PPi is converted to PPi-R, while the released indicator I can be transferred to the R-I complex by adding R without removing the components from the solution. The excess dNTP in each cycle is degraded by apyrase. Because there will be no ATP
produced in the detection steps, apyrase now primarily degrades dNTP and is more efficient in its action. Accordingly, more bases can be determined.
Another advantage of this method is that only two kinds of enzymes are used here rather than four, and the detection step will not adversely affect the other steps. However, this improved method cannot detect the bases in homopolymeric regions either, and so 3'-0 allyl dNTPs are employed to circumvent this problem.
References
1. Ronaghi M., Uhlen M, Nyren P. A sequencing method based on real-time pyrophosphate. Science 281(5375), 363-365
(1998) .
2. Ronaghi M., Karamohamed S., Pettersson B., Uhlen M., Nyren P. Real-time DNA sequencing using detection of pyrophosphate release. Anal. Biochem. 242(1), 84-89 (1996) .
3. "Design and Synthesis of a 3' -O-Allyloxy Photocleavable Fluorescent Nucleotide as a Reversible Terminator for DNA Sequencing By Synthesis" . H. Ruparel, L. Bi, Z. Li, X. Bai, D. H. Kim, N. Turro & J. Ju. Proceedings of the National Academy of Sciences USA 2005, 102, 5932-5937.
4. Edwards, J. R., Itagaki, Y. & Ju, J. Solid Phase Capturable Dideoxynucleotides for Multiplex Genotyping Using Mass Spectrometry (2001). Nucleic Acids Res. 29, elO4 (pl-6) . 5. Roge G. etc. An indicator displacement system for fluorescent detection of phosphate oxyanions under physiological conditions. Tetrahedron Letters 45(2004) 8721-872.4. 6. Ju, J. et al., U.S. Patent 6,664,079.
Claims
1. A method for determining the nucleotide sequence of a single-stranded DNA comprising performing the following steps for each nucleic acid residue of the DNA whose identity is to be determined:
(a) contacting the DNA under DNA polymerization- permitting conditions with (i) a 3' -0-blocked dNTP selected from the group consisting of 3'-O- blocked dATP, 3'-0-blocked dCTP, 3'-0-blocked dGTP, and 3'-0-blocked dTTP, and (ii) 9°N DNA polymerase (exo-) A4851/Y409V;
(b) (i) determining whether pyrophosphate is generated as a result of step (a), whereby (1) pyrophosphate generation indicates that polymerization has occurred and the identity of the nucleic acid residue in the DNA is that which is complementary to the 3'-0-blocked dNTP used in part (i) of step (a), and (2) the absence of pyrophosphate generation indicates that the identity of such nucleic acid residue is not that which is complementary to such 3'-0-blocked dNTP, and (ii) if pyrophosphate is not generated, repeating step (a) once, twice or three times as necessary, wherein in each repetition a 3'-O- blocked dNTP is used which is different from any 3'-0-blocked dNTP already used, and determining, after each repetition of step (a) , whether pyrophosphate is generated, such generation indicating that polymerization has occurred and the identity of the nucleic acid residue in the DNA is that which is complementary to the 3'-O- blocked dNTP used in part (i) of the repeated step (a) ; and
(c) removing from the 3'-0-blocked dNTP polymerized in step (a) or (b) , whichever is applicable, the moiety blocking the 3'-0 atom of the dNTP, with the proviso that such removing step is optional in the event that there remains no further nucleic acid residue of the DNA whose identity is to be determined.
2. The method of claim 1, wherein determining whether pyrophosphate generated in step (b) (i) is performed by detecting light generated by a luciferase-based reaction.
3. The method of claim 2, wherein the luciferase is firefly luciferase .
4. The method of claim 2, wherein the luciferase-based reaction comprises contacting the pyrophosphate with a sulfurylase under conditions permitting the generation of ATP from the pyrophosphate, and contacting the ATP so generated with a luciferase under conditions permitting the generation of light by the luciferase in the presence of ATP.
5. The method of claim 1, wherein determining whether pyrophosphate is generated in step (b) (i) is performed by detecting dissociation of a coumarin-derived indicator from a complex between the indicator and a bis-Zn2+-dipicolylamine coordination compound, wherein the coumarin-derived indicator has the following structure : and the bis-Zn2+-dipicolylamine coordination compound, when in association with the coumarin-derived indicator, has the following structure:
4NO3-
6. The method of claim 1, wherein the moiety blocking the 3'-0 atom of the dNTP is an allyl moiety or a methoxymethyl moiety.
7. The method of claim 6, wherein the moiety is an allyl moiety.
8. The method of claim 1, wherein the DNA is immobilized on a solid substrate.
9. The method of claim 8, wherein the DNA is immobilized on the solid substrate via an azido linkage, an alkynyl linkage, 1,3-dipolar cycloaddition linkage, or biotin- streptavidin interaction.
10. The method of claim 8, wherein the solid substrate is in the form of a chip, a bead, a well, a capillary tube, or a slide.
11. The method of claim 8, wherein the solid substrate is gold, quartz, silica, or plastic.
512. The method of claim 8, wherein the solid substrate is porous .
13. The method of claim 4, wherein the moiety blocking the 3'-0 atom of the dNTP is an allyl moiety and the single-
10 stranded DNA is immobilized to a solid substrate.
14. The method of claim 5, wherein the moiety blocking the 3'-0 atom of the dNTP is an allyl moiety and the single- stranded DNA is immobilized to a solid substrate.
15
15. The method ,of claim 1, wherein the moiety blocking the 3'-0 atom of the dNTP is an allyl moiety and removing it is performed using Na2PdCl4 and TPPTS.
2016. A compound comprising a dNTP having bound to its 3' oxygen an allyl or methoxymethyl moiety.
17. The compound of claim 16, wherein the moiety is an allyl moiety.
25
18. The compound of claim 16, wherein the moiety is a methoxymethyl moiety.
19. The compound of claim 16, wherein the dNTP is dATP. 30
20. The compound of claim 16, wherein the dNTP is dCTP.
21. The compound of claim 16, wherein the dNTP is dGTP.
22. The compound of claim 16, wherein the dNTP is dTTP.
23. The compound of claim 16, wherein the compound is a 3'- 5 O-allyl dNTP.
24. The compound of claim 23, wherein the compound is 3'-O- allyl dATP.
1025. The compound of claim 23, wherein the compound is 3'-O- allyl dCTP.
26. The compound of claim 23, wherein the compound is 3'-O- allyl dGTP.
15
27. The compound of claim 23, wherein the compound is 3'-O- allyl dTTP.
28. A compound comprising a dNTP having bound to its 3' 20 oxygen an allyl derivative or a methoxymethyl derivative .
29. A process for producing a 3' -O-allyl dNTP comprising:
(a) sequentially contacting a dimethoxytrityl (DMTr) 5 3' protected nucleoside triphosphate with (i) 3- bromo propene, NaOH and benzene, and (ii) a suitable solvent; and
(b) ' sequentially contacting the product of step (a) with (i) POCl3/ (MeO)3P (0) , (ii) tributylammonium 0 pyrophosphate, and (iii) TEAB/NH4OH, so as to produce the 3' -O-allyl dNTP.
30. The method of claim 29, wherein the suitable solvent of step (a) (ii) is 3% THF/CHC13.
31. The method of claim 29, wherein the concentration of 5 TEAB in step (b) (iii) is about 0.1M.
32. A kit for use in sequencing a single-stranded DNA comprising:
(a) 3'-O-allyl dATP, 3'-0-allyl dCTP, 3'-O-allyl dTTP, 10 and 3'-0-allyl dGTP, each in a separate compartment; and (b) instructions for use.
33. The kit of claim 32, further comprising a 9°N DNA 15 polymerase (exo-) A4851/Y409V.
34. The kit of claim 33, further comprising reagents permitting polymerization.
2035. The kit of claim 33, further comprising reagents permitting pyrophosphate detection using a luciferase- based reaction.
36. The kit of claim 33, further comprising reagents 25 permitting pyrophosphate detection using a coumarin- derived indicator.
37. The kit of claim 33, further comprising reagents permitting removal of an allyl group from a 3'-O~allyl
30 dNTP.
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Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7345159B2 (en) | 2000-10-06 | 2008-03-18 | The Trustees Of Columbia University In The City Of New York | Massive parallel method for decoding DNA and RNA |
US7622279B2 (en) | 2004-03-03 | 2009-11-24 | The Trustees Of Columbia University In The City Of New York | Photocleavable fluorescent nucleotides for DNA sequencing on chip constructed by site-specific coupling chemistry |
WO2009151921A1 (en) | 2008-05-27 | 2009-12-17 | Trilink Biotechnologies | Chemically modified nucleoside 5'-triphosphates for thermally initiated amplification of nucleic acid |
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WO2013096906A1 (en) | 2011-12-22 | 2013-06-27 | Life Technologies Corporation | Data compression of waveforms associated with a chemical event occuring on a sensor array |
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EP2657869A2 (en) | 2007-08-29 | 2013-10-30 | Applied Biosystems, LLC | Alternative nucleic acid sequencing methods |
US8692298B2 (en) | 2006-12-14 | 2014-04-08 | Life Technologies Corporation | Chemical sensor array having multiple sensors per well |
US8796432B2 (en) | 2005-10-31 | 2014-08-05 | The Trustees Of Columbia University In The City Of New York | Chemically cleavable 3'-o-allyl-DNTP-allyl-fluorophore fluorescent nucleotide analogues and related methods |
US8841217B1 (en) | 2013-03-13 | 2014-09-23 | Life Technologies Corporation | Chemical sensor with protruded sensor surface |
US8845880B2 (en) | 2010-12-22 | 2014-09-30 | Genia Technologies, Inc. | Nanopore-based single DNA molecule characterization, identification and isolation using speed bumps |
US8858782B2 (en) | 2010-06-30 | 2014-10-14 | Life Technologies Corporation | Ion-sensing charge-accumulation circuits and methods |
US8889348B2 (en) | 2006-06-07 | 2014-11-18 | The Trustees Of Columbia University In The City Of New York | DNA sequencing by nanopore using modified nucleotides |
US8912005B1 (en) | 2010-09-24 | 2014-12-16 | Life Technologies Corporation | Method and system for delta double sampling |
US8912580B2 (en) | 2009-05-29 | 2014-12-16 | Life Technologies Corporation | Active chemically-sensitive sensors with in-sensor current sources |
US8936763B2 (en) | 2008-10-22 | 2015-01-20 | Life Technologies Corporation | Integrated sensor arrays for biological and chemical analysis |
US8962242B2 (en) | 2011-01-24 | 2015-02-24 | Genia Technologies, Inc. | System for detecting electrical properties of a molecular complex |
US8962366B2 (en) | 2013-01-28 | 2015-02-24 | Life Technologies Corporation | Self-aligned well structures for low-noise chemical sensors |
US8963216B2 (en) | 2013-03-13 | 2015-02-24 | Life Technologies Corporation | Chemical sensor with sidewall spacer sensor surface |
US8986629B2 (en) | 2012-02-27 | 2015-03-24 | Genia Technologies, Inc. | Sensor circuit for controlling, detecting, and measuring a molecular complex |
US9041420B2 (en) | 2010-02-08 | 2015-05-26 | Genia Technologies, Inc. | Systems and methods for characterizing a molecule |
US9051612B2 (en) | 2006-09-28 | 2015-06-09 | Illumina, Inc. | Compositions and methods for nucleotide sequencing |
US9110478B2 (en) | 2011-01-27 | 2015-08-18 | Genia Technologies, Inc. | Temperature regulation of measurement arrays |
US9116117B2 (en) | 2013-03-15 | 2015-08-25 | Life Technologies Corporation | Chemical sensor with sidewall sensor surface |
US9115163B2 (en) | 2007-10-19 | 2015-08-25 | The Trustees Of Columbia University In The City Of New York | DNA sequence with non-fluorescent nucleotide reversible terminators and cleavable label modified nucleotide terminators |
US9128044B2 (en) | 2013-03-15 | 2015-09-08 | Life Technologies Corporation | Chemical sensors with consistent sensor surface areas |
US9164070B2 (en) | 2010-06-30 | 2015-10-20 | Life Technologies Corporation | Column adc |
US9169510B2 (en) | 2005-06-21 | 2015-10-27 | The Trustees Of Columbia University In The City Of New York | Pyrosequencing methods and related compositions |
US9175342B2 (en) | 2007-10-19 | 2015-11-03 | The Trustees Of Columbia University In The City Of New York | Synthesis of cleavable fluorescent nucleotides as reversible terminators for DNA sequencing by synthesis |
US9194000B2 (en) | 2008-06-25 | 2015-11-24 | Life Technologies Corporation | Methods and apparatus for measuring analytes using large scale FET arrays |
US9255292B2 (en) | 2005-10-31 | 2016-02-09 | The Trustees Of Columbia University In The City Of New York | Synthesis of four-color 3′-O-allyl modified photocleavable fluorescent nucleotides and related methods |
US9270264B2 (en) | 2012-05-29 | 2016-02-23 | Life Technologies Corporation | System for reducing noise in a chemical sensor array |
US9322062B2 (en) | 2013-10-23 | 2016-04-26 | Genia Technologies, Inc. | Process for biosensor well formation |
US9388193B2 (en) | 2014-05-22 | 2016-07-12 | National Health Research Institutes | Dipicolylamine derivatives and their pharmaceutical uses |
US9404920B2 (en) | 2006-12-14 | 2016-08-02 | Life Technologies Corporation | Methods and apparatus for detecting molecular interactions using FET arrays |
US9494554B2 (en) | 2012-06-15 | 2016-11-15 | Genia Technologies, Inc. | Chip set-up and high-accuracy nucleic acid sequencing |
US9515676B2 (en) | 2012-01-31 | 2016-12-06 | Life Technologies Corporation | Methods and computer program products for compression of sequencing data |
US9551697B2 (en) | 2013-10-17 | 2017-01-24 | Genia Technologies, Inc. | Non-faradaic, capacitively coupled measurement in a nanopore cell array |
US9605309B2 (en) | 2012-11-09 | 2017-03-28 | Genia Technologies, Inc. | Nucleic acid sequencing using tags |
US9605307B2 (en) | 2010-02-08 | 2017-03-28 | Genia Technologies, Inc. | Systems and methods for forming a nanopore in a lipid bilayer |
US9618475B2 (en) | 2010-09-15 | 2017-04-11 | Life Technologies Corporation | Methods and apparatus for measuring analytes |
US9671363B2 (en) | 2013-03-15 | 2017-06-06 | Life Technologies Corporation | Chemical sensor with consistent sensor surface areas |
US9678055B2 (en) | 2010-02-08 | 2017-06-13 | Genia Technologies, Inc. | Methods for forming a nanopore in a lipid bilayer |
US9708358B2 (en) | 2000-10-06 | 2017-07-18 | The Trustees Of Columbia University In The City Of New York | Massive parallel method for decoding DNA and RNA |
US9759711B2 (en) | 2013-02-05 | 2017-09-12 | Genia Technologies, Inc. | Nanopore arrays |
US9823217B2 (en) | 2013-03-15 | 2017-11-21 | Life Technologies Corporation | Chemical device with thin conductive element |
US9835585B2 (en) | 2013-03-15 | 2017-12-05 | Life Technologies Corporation | Chemical sensor with protruded sensor surface |
US9841398B2 (en) | 2013-01-08 | 2017-12-12 | Life Technologies Corporation | Methods for manufacturing well structures for low-noise chemical sensors |
US9852919B2 (en) | 2013-01-04 | 2017-12-26 | Life Technologies Corporation | Methods and systems for point of use removal of sacrificial material |
US9864846B2 (en) | 2012-01-31 | 2018-01-09 | Life Technologies Corporation | Methods and computer program products for compression of sequencing data |
US9890426B2 (en) | 2015-03-09 | 2018-02-13 | The Trustees Of Columbia University In The City Of New York | Pore-forming protein conjugate compositions and methods |
US9960253B2 (en) | 2010-07-03 | 2018-05-01 | Life Technologies Corporation | Chemically sensitive sensor with lightly doped drains |
US9970984B2 (en) | 2011-12-01 | 2018-05-15 | Life Technologies Corporation | Method and apparatus for identifying defects in a chemical sensor array |
US10077472B2 (en) | 2014-12-18 | 2018-09-18 | Life Technologies Corporation | High data rate integrated circuit with power management |
US10100357B2 (en) | 2013-05-09 | 2018-10-16 | Life Technologies Corporation | Windowed sequencing |
WO2019027326A2 (en) | 2017-08-04 | 2019-02-07 | Universiteit Leiden | Screening method |
US10246479B2 (en) | 2012-04-09 | 2019-04-02 | The Trustees Of Columbia University In The City Of New York | Method of preparation of nanopore and uses thereof |
US10379079B2 (en) | 2014-12-18 | 2019-08-13 | Life Technologies Corporation | Methods and apparatus for measuring analytes using large scale FET arrays |
US10421995B2 (en) | 2013-10-23 | 2019-09-24 | Genia Technologies, Inc. | High speed molecular sensing with nanopores |
US10458942B2 (en) | 2013-06-10 | 2019-10-29 | Life Technologies Corporation | Chemical sensor array having multiple sensors per well |
US10605767B2 (en) | 2014-12-18 | 2020-03-31 | Life Technologies Corporation | High data rate integrated circuit with transmitter configuration |
US10648026B2 (en) | 2013-03-15 | 2020-05-12 | The Trustees Of Columbia University In The City Of New York | Raman cluster tagged molecules for biological imaging |
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US11231451B2 (en) | 2010-06-30 | 2022-01-25 | Life Technologies Corporation | Methods and apparatus for testing ISFET arrays |
WO2022019837A1 (en) * | 2020-07-21 | 2022-01-27 | Agency For Science, Technology And Research | A pyrosequencing method |
US11307166B2 (en) | 2010-07-01 | 2022-04-19 | Life Technologies Corporation | Column ADC |
US11339430B2 (en) | 2007-07-10 | 2022-05-24 | Life Technologies Corporation | Methods and apparatus for measuring analytes using large scale FET arrays |
Families Citing this family (13)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2012083249A2 (en) | 2010-12-17 | 2012-06-21 | The Trustees Of Columbia University In The City Of New York | Dna sequencing by synthesis using modified nucleotides and nanopore detection |
WO2012162429A2 (en) | 2011-05-23 | 2012-11-29 | The Trustees Of Columbia University In The City Of New York | Dna sequencing by synthesis using raman and infrared spectroscopy detection |
US8597882B2 (en) * | 2012-02-03 | 2013-12-03 | Pyrobett Pte. Ltd. | Method and apparatus for conducting an assay |
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EP3596099B1 (en) | 2017-03-06 | 2024-07-24 | Singular Genomics Systems, Inc. | Nucleic acid sequencing-by-synthesis (sbs) methods that combine sbs cycle steps |
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WO2019178302A1 (en) * | 2018-03-13 | 2019-09-19 | Innovasion Labs, Inc. | Methods for single molecule sequencing |
US10738072B1 (en) | 2018-10-25 | 2020-08-11 | Singular Genomics Systems, Inc. | Nucleotide analogues |
CN113453692A (en) | 2019-01-08 | 2021-09-28 | 奇异基因组学系统公司 | Nucleotide cleavable linkers and uses thereof |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6210891B1 (en) * | 1996-09-27 | 2001-04-03 | Pyrosequencing Ab | Method of sequencing DNA |
WO2004018497A2 (en) * | 2002-08-23 | 2004-03-04 | Solexa Limited | Modified nucleotides for polynucleotide sequencing |
Family Cites Families (106)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4711955A (en) | 1981-04-17 | 1987-12-08 | Yale University | Modified nucleotides and methods of preparing and using same |
US5118605A (en) | 1984-10-16 | 1992-06-02 | Chiron Corporation | Polynucleotide determination with selectable cleavage sites |
US4824775A (en) | 1985-01-03 | 1989-04-25 | Molecular Diagnostics, Inc. | Cells labeled with multiple Fluorophores bound to a nucleic acid carrier |
US4772691A (en) | 1985-06-05 | 1988-09-20 | The Medical College Of Wisconsin, Inc. | Chemically cleavable nucleotides |
DE3529478A1 (en) | 1985-08-16 | 1987-02-19 | Boehringer Mannheim Gmbh | 7-DESAZA-2'DESOXYGUANOSINE NUCLEOTIDES, METHOD FOR THE PRODUCTION THEREOF AND THEIR USE FOR NUCLEIC ACID SEQUENCING |
US5151507A (en) | 1986-07-02 | 1992-09-29 | E. I. Du Pont De Nemours And Company | Alkynylamino-nucleotides |
US5047519A (en) | 1986-07-02 | 1991-09-10 | E. I. Du Pont De Nemours And Company | Alkynylamino-nucleotides |
US5242796A (en) | 1986-07-02 | 1993-09-07 | E. I. Du Pont De Nemours And Company | Method, system and reagents for DNA sequencing |
DE68928853T2 (en) | 1988-05-20 | 1999-08-05 | Cetus Corp., Emeryville, Calif. | FASTENING OF SEQUENCE-SPECIFIC SAMPLES |
US5174962A (en) | 1988-06-20 | 1992-12-29 | Genomyx, Inc. | Apparatus for determining DNA sequences by mass spectrometry |
US5547839A (en) | 1989-06-07 | 1996-08-20 | Affymax Technologies N.V. | Sequencing of surface immobilized polymers utilizing microflourescence detection |
US5302509A (en) | 1989-08-14 | 1994-04-12 | Beckman Instruments, Inc. | Method for sequencing polynucleotides |
EP0450060A1 (en) | 1989-10-26 | 1991-10-09 | Sri International | Dna sequencing |
GB9208733D0 (en) | 1992-04-22 | 1992-06-10 | Medical Res Council | Dna sequencing method |
US5383858B1 (en) | 1992-08-17 | 1996-10-29 | Medrad Inc | Front-loading medical injector and syringe for use therewith |
US6074823A (en) | 1993-03-19 | 2000-06-13 | Sequenom, Inc. | DNA sequencing by mass spectrometry via exonuclease degradation |
GB9315847D0 (en) | 1993-07-30 | 1993-09-15 | Isis Innovation | Tag reagent and assay method |
CA2170264A1 (en) | 1993-09-10 | 1995-03-16 | Michael W. Konrad | Optical detection of position of oligonucleotides on large dna molecules |
DE69426731T2 (en) | 1993-11-17 | 2001-06-28 | Amersham Pharmacia Biotech Uk Ltd., Little Chalfont | METHOD FOR MASS SPECTROSCOPIC SEQUENCE ANALYSIS OF A NUCLEIC ACID BY PRIMER EXTENSION |
US6028190A (en) | 1994-02-01 | 2000-02-22 | The Regents Of The University Of California | Probes labeled with energy transfer coupled dyes |
US5654419A (en) | 1994-02-01 | 1997-08-05 | The Regents Of The University Of California | Fluorescent labels and their use in separations |
US5869255A (en) | 1994-02-01 | 1999-02-09 | The Regents Of The University Of California | Probes labeled with energy transfer couples dyes exemplified with DNA fragment analysis |
US5645419A (en) * | 1994-03-29 | 1997-07-08 | Tokyo Electron Kabushiki Kaisha | Heat treatment method and device |
US5552278A (en) | 1994-04-04 | 1996-09-03 | Spectragen, Inc. | DNA sequencing by stepwise ligation and cleavage |
US6589736B1 (en) | 1994-11-22 | 2003-07-08 | The Trustees Of Boston University | Photocleavable agents and conjugates for the detection and isolation of biomolecules |
US20020168642A1 (en) | 1994-06-06 | 2002-11-14 | Andrzej Drukier | Sequencing duplex DNA by mass spectroscopy |
US6232465B1 (en) | 1994-09-02 | 2001-05-15 | Andrew C. Hiatt | Compositions for enzyme catalyzed template-independent creation of phosphodiester bonds using protected nucleotides |
US5872244A (en) | 1994-09-02 | 1999-02-16 | Andrew C. Hiatt | 3' protected nucleotides for enzyme catalyzed template-independent creation of phosphodiester bonds |
US6214987B1 (en) | 1994-09-02 | 2001-04-10 | Andrew C. Hiatt | Compositions for enzyme catalyzed template-independent formation of phosphodiester bonds using protected nucleotides |
US5763594A (en) | 1994-09-02 | 1998-06-09 | Andrew C. Hiatt | 3' protected nucleotides for enzyme catalyzed template-independent creation of phosphodiester bonds |
US5808045A (en) | 1994-09-02 | 1998-09-15 | Andrew C. Hiatt | Compositions for enzyme catalyzed template-independent creation of phosphodiester bonds using protected nucleotides |
DE4438918A1 (en) | 1994-11-04 | 1996-05-09 | Hoechst Ag | Modified oligonucleotides, their preparation and their use |
AU5171696A (en) | 1995-02-27 | 1996-09-18 | Ely Michael Rabani | Device, compounds, algorithms, and methods of molecular characterization and manipulation with molecular parallelism |
EP0745686A1 (en) | 1995-06-01 | 1996-12-04 | Roche Diagnostics GmbH | The use of DNA polymerase 3'-intrinsic editing activity |
US5728528A (en) | 1995-09-20 | 1998-03-17 | The Regents Of The University Of California | Universal spacer/energy transfer dyes |
US5945283A (en) | 1995-12-18 | 1999-08-31 | Washington University | Methods and kits for nucleic acid analysis using fluorescence resonance energy transfer |
US6312893B1 (en) | 1996-01-23 | 2001-11-06 | Qiagen Genomics, Inc. | Methods and compositions for determining the sequence of nucleic acid molecules |
US6613508B1 (en) | 1996-01-23 | 2003-09-02 | Qiagen Genomics, Inc. | Methods and compositions for analyzing nucleic acid molecules utilizing sizing techniques |
EP0992511B1 (en) | 1996-01-23 | 2009-03-11 | Operon Biotechnologies, Inc. | Methods and compositions for determining the sequence of nucleic acid molecules |
US5843203A (en) * | 1996-03-22 | 1998-12-01 | Grantek, Inc. | Agricultural carrier |
US6361940B1 (en) | 1996-09-24 | 2002-03-26 | Qiagen Genomics, Inc. | Compositions and methods for enhancing hybridization and priming specificity |
US5853992A (en) | 1996-10-04 | 1998-12-29 | The Regents Of The University Of California | Cyanine dyes with high-absorbance cross section as donor chromophores in energy transfer labels |
US5885775A (en) | 1996-10-04 | 1999-03-23 | Perseptive Biosystems, Inc. | Methods for determining sequences information in polynucleotides using mass spectrometry |
ATE319855T1 (en) | 1996-12-10 | 2006-03-15 | Sequenom Inc | SEPARABLE, NON-VOLATILE MOLECULES FOR MASS MARKING |
US6046005A (en) | 1997-01-15 | 2000-04-04 | Incyte Pharmaceuticals, Inc. | Nucleic acid sequencing with solid phase capturable terminators comprising a cleavable linking group |
US5804386A (en) | 1997-01-15 | 1998-09-08 | Incyte Pharmaceuticals, Inc. | Sets of labeled energy transfer fluorescent primers and their use in multi component analysis |
US5876936A (en) | 1997-01-15 | 1999-03-02 | Incyte Pharmaceuticals, Inc. | Nucleic acid sequencing with solid phase capturable terminators |
WO1998033939A1 (en) | 1997-01-31 | 1998-08-06 | Hitachi, Ltd. | Method for determining nucleic acid base sequence and apparatus therefor |
US6197557B1 (en) | 1997-03-05 | 2001-03-06 | The Regents Of The University Of Michigan | Compositions and methods for analysis of nucleic acids |
US5834203A (en) | 1997-08-25 | 1998-11-10 | Applied Spectral Imaging | Method for classification of pixels into groups according to their spectra using a plurality of wide band filters and hardwire therefore |
WO1999049082A2 (en) | 1998-03-23 | 1999-09-30 | Invitrogen Corporation | Modified nucleotides and methods useful for nucleic acid sequencing |
WO1999057321A1 (en) | 1998-05-01 | 1999-11-11 | Arizona Board Of Regents | Method of determining the nucleotide sequence of oligonucleotides and dna molecules |
US5948648A (en) | 1998-05-29 | 1999-09-07 | Khan; Shaheer H. | Nucleotide compounds including a rigid linker |
US6218530B1 (en) | 1998-06-02 | 2001-04-17 | Ambergen Inc. | Compounds and methods for detecting biomolecules |
US6218118B1 (en) | 1998-07-09 | 2001-04-17 | Agilent Technologies, Inc. | Method and mixture reagents for analyzing the nucleotide sequence of nucleic acids by mass spectrometry |
US6787308B2 (en) | 1998-07-30 | 2004-09-07 | Solexa Ltd. | Arrayed biomolecules and their use in sequencing |
WO2000036151A1 (en) | 1998-12-14 | 2000-06-22 | Li-Cor, Inc. | A heterogeneous assay for pyrophosphate detection |
US20030054360A1 (en) | 1999-01-19 | 2003-03-20 | Larry Gold | Method and apparatus for the automated generation of nucleic acid ligands |
EP1159453B1 (en) | 1999-03-10 | 2008-05-28 | ASM Scientific, Inc. | A method for direct nucleic acid sequencing |
US6316230B1 (en) | 1999-08-13 | 2001-11-13 | Applera Corporation | Polymerase extension at 3′ terminus of PNA-DNA chimera |
WO2001012776A2 (en) | 1999-08-16 | 2001-02-22 | Human Genome Sciences, Inc. | 18 human secreted proteins |
US6664399B1 (en) | 1999-09-02 | 2003-12-16 | E. I. Du Pont De Nemours & Company | Triazole linked carbohydrates |
AU7537200A (en) | 1999-09-29 | 2001-04-30 | Solexa Ltd. | Polynucleotide sequencing |
EP1221052B1 (en) | 1999-10-08 | 2010-03-17 | Robert C. Leif | Conjugated polymer tag complexes |
GB0013276D0 (en) | 2000-06-01 | 2000-07-26 | Amersham Pharm Biotech Uk Ltd | Nucleotide analogues |
ATE384731T1 (en) | 2000-08-03 | 2008-02-15 | Hoffmann La Roche | NUCLEIC ACID BINDING COMPOUNDS WITH PYRAZOLO 3, 4-D PYRIMIDINE ANALOGUES OF PURINE-2,6-DIAMINE AND THEIR USE |
US20060057565A1 (en) | 2000-09-11 | 2006-03-16 | Jingyue Ju | Combinatorial fluorescence energy transfer tags and uses thereof |
WO2002022883A1 (en) | 2000-09-11 | 2002-03-21 | The Trustees Of Columbia University In The City Of New York | Combinatorial fluorescence energy transfer tags and uses thereof |
US6627748B1 (en) | 2000-09-11 | 2003-09-30 | The Trustees Of Columbia University In The City Of New York | Combinatorial fluorescence energy transfer tags and their applications for multiplex genetic analyses |
EP1337541B1 (en) | 2000-10-06 | 2007-03-07 | The Trustees of Columbia University in the City of New York | Massive parallel method for decoding DNA and RNA |
US7211414B2 (en) * | 2000-12-01 | 2007-05-01 | Visigen Biotechnologies, Inc. | Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity |
US20030027140A1 (en) | 2001-03-30 | 2003-02-06 | Jingyue Ju | High-fidelity DNA sequencing using solid phase capturable dideoxynucleotides and mass spectrometry |
US6573677B2 (en) * | 2001-06-18 | 2003-06-03 | Motorola, Inc. | Method of compensating for abrupt load changes in an anti-pinch window control system |
US6613523B2 (en) | 2001-06-29 | 2003-09-02 | Agilent Technologies, Inc. | Method of DNA sequencing using cleavable tags |
US7057031B2 (en) | 2001-07-13 | 2006-06-06 | Ambergen, Inc. | Nucleotide compositions comprising photocleavable markers and methods of preparation thereof |
US6902904B2 (en) | 2001-08-27 | 2005-06-07 | Pharmanetics Incorporated | Coagulation assay reagents containing lanthanides |
US7057026B2 (en) | 2001-12-04 | 2006-06-06 | Solexa Limited | Labelled nucleotides |
EP1572902B1 (en) | 2002-02-01 | 2014-06-11 | Life Technologies Corporation | HIGH POTENCY siRNAS FOR REDUCING THE EXPRESSION OF TARGET GENES |
US7074597B2 (en) | 2002-07-12 | 2006-07-11 | The Trustees Of Columbia University In The City Of New York | Multiplex genotyping using solid phase capturable dideoxynucleotides and mass spectrometry |
EP3438116B1 (en) | 2002-08-23 | 2021-02-17 | Illumina Cambridge Limited | Labelled nucleotides |
US20050032081A1 (en) | 2002-12-13 | 2005-02-10 | Jingyue Ju | Biomolecular coupling methods using 1,3-dipolar cycloaddition chemistry |
US20060252938A1 (en) | 2003-04-28 | 2006-11-09 | Basf Aktiengesellschaft | Process for the separation of palladium catalyst from crude reaction mixtures of aryl acetic acids obtained by carbonylation |
GB0321306D0 (en) | 2003-09-11 | 2003-10-15 | Solexa Ltd | Modified polymerases for improved incorporation of nucleotide analogues |
US7622026B2 (en) * | 2004-03-02 | 2009-11-24 | Panasonic Corporation | Biosensor |
EP2436778A3 (en) | 2004-03-03 | 2012-07-11 | The Trustees of Columbia University in the City of New York | Photocleavable fluorescent nucleotides for DNA sequencing on chip constructed by site-specific coupling chemistry |
US20050239134A1 (en) | 2004-04-21 | 2005-10-27 | Board Of Regents, The University Of Texas System | Combinatorial selection of phosphorothioate single-stranded DNA aptamers for TGF-beta-1 protein |
WO2006073436A2 (en) | 2004-04-29 | 2006-07-13 | The Trustees Of Columbia University In The City Of New York | Mass tag pcr for multiplex diagnostics |
US20060105461A1 (en) | 2004-10-22 | 2006-05-18 | May Tom-Moy | Nanopore analysis system |
US9169510B2 (en) | 2005-06-21 | 2015-10-27 | The Trustees Of Columbia University In The City Of New York | Pyrosequencing methods and related compositions |
WO2007053719A2 (en) | 2005-10-31 | 2007-05-10 | The Trustees Of Columbia University In The City Of New York | Chemically cleavable 3'-o-allyl-dntp-allyl-fluorophore fluorescent nucleotide analogues and related methods |
WO2007053702A2 (en) | 2005-10-31 | 2007-05-10 | The Trustees Of Columbia University In The City Of New York | Synthesis of four color 3'-o-allyl modified photocleavable fluorescent nucleotides and related methods |
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US8889348B2 (en) | 2006-06-07 | 2014-11-18 | The Trustees Of Columbia University In The City Of New York | DNA sequencing by nanopore using modified nucleotides |
US8399188B2 (en) | 2006-09-28 | 2013-03-19 | Illumina, Inc. | Compositions and methods for nucleotide sequencing |
DE112007002932B4 (en) | 2006-12-01 | 2015-08-06 | The Trustees Of Columbia University In The City Of New York | Four-color DNA sequencing by synthesis using cleavable, reversible, fluorescent nucleotide terminators |
WO2009051807A1 (en) | 2007-10-19 | 2009-04-23 | The Trustees Of Columbia University In The City Of New York | Design and synthesis of cleavable fluorescent nucleotides as reversible terminators for dna sequencing by synthesis |
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WO2012083249A2 (en) | 2010-12-17 | 2012-06-21 | The Trustees Of Columbia University In The City Of New York | Dna sequencing by synthesis using modified nucleotides and nanopore detection |
WO2012162429A2 (en) | 2011-05-23 | 2012-11-29 | The Trustees Of Columbia University In The City Of New York | Dna sequencing by synthesis using raman and infrared spectroscopy detection |
WO2013154999A2 (en) | 2012-04-09 | 2013-10-17 | The Trustees Of Columbia University In The City Of New York | Method of preparation of nanopore and uses thereof |
EP2864502B1 (en) | 2012-06-20 | 2019-10-23 | The Trustees of Columbia University in the City of New York | Nucleic acid sequencing by nanopore detection of tag molecules |
US10648026B2 (en) | 2013-03-15 | 2020-05-12 | The Trustees Of Columbia University In The City Of New York | Raman cluster tagged molecules for biological imaging |
CN105102627B (en) | 2013-03-15 | 2018-10-19 | 纽约哥伦比亚大学理事会 | Method for detecting a variety of predetermined compounds in sample |
US9159610B2 (en) | 2013-10-23 | 2015-10-13 | Globalfoundires, Inc. | Hybrid manganese and manganese nitride barriers for back-end-of-line metallization and methods for fabricating the same |
US10718011B2 (en) | 2014-02-12 | 2020-07-21 | The Trustees Of Columbia University In The City Of New York | Single molecule electronic multiplex SNP assay and PCR analysis |
US10240195B2 (en) | 2014-03-24 | 2019-03-26 | The Trustees Of Columbia University In The City Of New York | Chemical methods for producing tagged nucleotides |
-
2006
- 2006-06-20 US US11/922,385 patent/US9169510B2/en not_active Expired - Fee Related
- 2006-06-20 WO PCT/US2006/024157 patent/WO2007002204A2/en active Application Filing
-
2015
- 2015-09-10 US US14/850,705 patent/US9909177B2/en not_active Expired - Fee Related
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6210891B1 (en) * | 1996-09-27 | 2001-04-03 | Pyrosequencing Ab | Method of sequencing DNA |
WO2004018497A2 (en) * | 2002-08-23 | 2004-03-04 | Solexa Limited | Modified nucleotides for polynucleotide sequencing |
Non-Patent Citations (1)
Title |
---|
TETRAHEDRON LETTERS vol. 45, 2004, pages 8721 - 8724 * |
Cited By (174)
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US9051612B2 (en) | 2006-09-28 | 2015-06-09 | Illumina, Inc. | Compositions and methods for nucleotide sequencing |
US9469873B2 (en) | 2006-09-28 | 2016-10-18 | Illumina, Inc. | Compositions and methods for nucleotide sequencing |
US7883869B2 (en) | 2006-12-01 | 2011-02-08 | The Trustees Of Columbia University In The City Of New York | Four-color DNA sequencing by synthesis using cleavable fluorescent nucleotide reversible terminators |
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US8890216B2 (en) | 2006-12-14 | 2014-11-18 | Life Technologies Corporation | Methods and apparatus for measuring analytes using large scale FET arrays |
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US10144961B2 (en) | 2007-10-19 | 2018-12-04 | The Trustees Of Columbia University In The City Of New York | Synthesis of cleavable fluorescent nucleotides as reversible terminators for DNA sequencing by synthesis |
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US9377437B2 (en) | 2010-02-08 | 2016-06-28 | Genia Technologies, Inc. | Systems and methods for characterizing a molecule |
US10343350B2 (en) | 2010-02-08 | 2019-07-09 | Genia Technologies, Inc. | Systems and methods for forming a nanopore in a lipid bilayer |
US11027502B2 (en) | 2010-02-08 | 2021-06-08 | Roche Sequencing Solutions, Inc. | Systems and methods for forming a nanopore in a lipid bilayer |
US9041420B2 (en) | 2010-02-08 | 2015-05-26 | Genia Technologies, Inc. | Systems and methods for characterizing a molecule |
US9678055B2 (en) | 2010-02-08 | 2017-06-13 | Genia Technologies, Inc. | Methods for forming a nanopore in a lipid bilayer |
US10371692B2 (en) | 2010-02-08 | 2019-08-06 | Genia Technologies, Inc. | Systems for forming a nanopore in a lipid bilayer |
US9605307B2 (en) | 2010-02-08 | 2017-03-28 | Genia Technologies, Inc. | Systems and methods for forming a nanopore in a lipid bilayer |
US11231451B2 (en) | 2010-06-30 | 2022-01-25 | Life Technologies Corporation | Methods and apparatus for testing ISFET arrays |
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US12050195B2 (en) | 2010-09-15 | 2024-07-30 | Life Technologies Corporation | Methods and apparatus for measuring analytes using chemfet arrays |
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US9958414B2 (en) | 2010-09-15 | 2018-05-01 | Life Technologies Corporation | Apparatus for measuring analytes including chemical sensor array |
US9618475B2 (en) | 2010-09-15 | 2017-04-11 | Life Technologies Corporation | Methods and apparatus for measuring analytes |
US8912005B1 (en) | 2010-09-24 | 2014-12-16 | Life Technologies Corporation | Method and system for delta double sampling |
US9110015B2 (en) | 2010-09-24 | 2015-08-18 | Life Technologies Corporation | Method and system for delta double sampling |
US8845880B2 (en) | 2010-12-22 | 2014-09-30 | Genia Technologies, Inc. | Nanopore-based single DNA molecule characterization, identification and isolation using speed bumps |
US9617593B2 (en) | 2010-12-22 | 2017-04-11 | Genia Technologies, Inc. | Nanopore-based single DNA molecule characterization, identification and isolation using speed bumps |
US10400278B2 (en) | 2010-12-22 | 2019-09-03 | Genia Technologies, Inc. | Nanopore-based single DNA molecule characterization, identification and isolation using speed bumps |
US9121059B2 (en) | 2010-12-22 | 2015-09-01 | Genia Technologies, Inc. | Nanopore-based single molecule characterization |
US10920271B2 (en) | 2010-12-22 | 2021-02-16 | Roche Sequencing Solutions, Inc. | Nanopore-based single DNA molecule characterization, identification and isolation using speed bumps |
US10156541B2 (en) | 2011-01-24 | 2018-12-18 | Genia Technologies, Inc. | System for detecting electrical properties of a molecular complex |
US8962242B2 (en) | 2011-01-24 | 2015-02-24 | Genia Technologies, Inc. | System for detecting electrical properties of a molecular complex |
US9581563B2 (en) | 2011-01-24 | 2017-02-28 | Genia Technologies, Inc. | System for communicating information from an array of sensors |
US10010852B2 (en) | 2011-01-27 | 2018-07-03 | Genia Technologies, Inc. | Temperature regulation of measurement arrays |
US9110478B2 (en) | 2011-01-27 | 2015-08-18 | Genia Technologies, Inc. | Temperature regulation of measurement arrays |
US10365321B2 (en) | 2011-12-01 | 2019-07-30 | Life Technologies Corporation | Method and apparatus for identifying defects in a chemical sensor array |
US9970984B2 (en) | 2011-12-01 | 2018-05-15 | Life Technologies Corporation | Method and apparatus for identifying defects in a chemical sensor array |
US10598723B2 (en) | 2011-12-01 | 2020-03-24 | Life Technologies Corporation | Method and apparatus for identifying defects in a chemical sensor array |
WO2013096897A1 (en) | 2011-12-21 | 2013-06-27 | Life Technologies Corporation | Method and apparatus for calibration of a sensor array |
WO2013096906A1 (en) | 2011-12-22 | 2013-06-27 | Life Technologies Corporation | Data compression of waveforms associated with a chemical event occuring on a sensor array |
US9864846B2 (en) | 2012-01-31 | 2018-01-09 | Life Technologies Corporation | Methods and computer program products for compression of sequencing data |
US9515676B2 (en) | 2012-01-31 | 2016-12-06 | Life Technologies Corporation | Methods and computer program products for compression of sequencing data |
US11275052B2 (en) | 2012-02-27 | 2022-03-15 | Roche Sequencing Solutions, Inc. | Sensor circuit for controlling, detecting, and measuring a molecular complex |
US8986629B2 (en) | 2012-02-27 | 2015-03-24 | Genia Technologies, Inc. | Sensor circuit for controlling, detecting, and measuring a molecular complex |
US11795191B2 (en) | 2012-04-09 | 2023-10-24 | The Trustees Of Columbia University In The City Of New York | Method of preparation of nanopore and uses thereof |
US10246479B2 (en) | 2012-04-09 | 2019-04-02 | The Trustees Of Columbia University In The City Of New York | Method of preparation of nanopore and uses thereof |
US9270264B2 (en) | 2012-05-29 | 2016-02-23 | Life Technologies Corporation | System for reducing noise in a chemical sensor array |
US9985624B2 (en) | 2012-05-29 | 2018-05-29 | Life Technologies Corporation | System for reducing noise in a chemical sensor array |
US10404249B2 (en) | 2012-05-29 | 2019-09-03 | Life Technologies Corporation | System for reducing noise in a chemical sensor array |
US9494554B2 (en) | 2012-06-15 | 2016-11-15 | Genia Technologies, Inc. | Chip set-up and high-accuracy nucleic acid sequencing |
US9605309B2 (en) | 2012-11-09 | 2017-03-28 | Genia Technologies, Inc. | Nucleic acid sequencing using tags |
US10822650B2 (en) | 2012-11-09 | 2020-11-03 | Roche Sequencing Solutions, Inc. | Nucleic acid sequencing using tags |
US9852919B2 (en) | 2013-01-04 | 2017-12-26 | Life Technologies Corporation | Methods and systems for point of use removal of sacrificial material |
US9841398B2 (en) | 2013-01-08 | 2017-12-12 | Life Technologies Corporation | Methods for manufacturing well structures for low-noise chemical sensors |
US10436742B2 (en) | 2013-01-08 | 2019-10-08 | Life Technologies Corporation | Methods for manufacturing well structures for low-noise chemical sensors |
US8962366B2 (en) | 2013-01-28 | 2015-02-24 | Life Technologies Corporation | Self-aligned well structures for low-noise chemical sensors |
US10012637B2 (en) | 2013-02-05 | 2018-07-03 | Genia Technologies, Inc. | Nanopore arrays |
US10809244B2 (en) | 2013-02-05 | 2020-10-20 | Roche Sequencing Solutions, Inc. | Nanopore arrays |
US9759711B2 (en) | 2013-02-05 | 2017-09-12 | Genia Technologies, Inc. | Nanopore arrays |
US8963216B2 (en) | 2013-03-13 | 2015-02-24 | Life Technologies Corporation | Chemical sensor with sidewall spacer sensor surface |
US8841217B1 (en) | 2013-03-13 | 2014-09-23 | Life Technologies Corporation | Chemical sensor with protruded sensor surface |
US9995708B2 (en) | 2013-03-13 | 2018-06-12 | Life Technologies Corporation | Chemical sensor with sidewall spacer sensor surface |
US9823217B2 (en) | 2013-03-15 | 2017-11-21 | Life Technologies Corporation | Chemical device with thin conductive element |
US10648026B2 (en) | 2013-03-15 | 2020-05-12 | The Trustees Of Columbia University In The City Of New York | Raman cluster tagged molecules for biological imaging |
US9671363B2 (en) | 2013-03-15 | 2017-06-06 | Life Technologies Corporation | Chemical sensor with consistent sensor surface areas |
US10422767B2 (en) | 2013-03-15 | 2019-09-24 | Life Technologies Corporation | Chemical sensor with consistent sensor surface areas |
US9116117B2 (en) | 2013-03-15 | 2015-08-25 | Life Technologies Corporation | Chemical sensor with sidewall sensor surface |
US9835585B2 (en) | 2013-03-15 | 2017-12-05 | Life Technologies Corporation | Chemical sensor with protruded sensor surface |
US9128044B2 (en) | 2013-03-15 | 2015-09-08 | Life Technologies Corporation | Chemical sensors with consistent sensor surface areas |
US10655175B2 (en) | 2013-05-09 | 2020-05-19 | Life Technologies Corporation | Windowed sequencing |
US11028438B2 (en) | 2013-05-09 | 2021-06-08 | Life Technologies Corporation | Windowed sequencing |
US10100357B2 (en) | 2013-05-09 | 2018-10-16 | Life Technologies Corporation | Windowed sequencing |
US10458942B2 (en) | 2013-06-10 | 2019-10-29 | Life Technologies Corporation | Chemical sensor array having multiple sensors per well |
US11774401B2 (en) | 2013-06-10 | 2023-10-03 | Life Technologies Corporation | Chemical sensor array having multiple sensors per well |
US11499938B2 (en) | 2013-06-10 | 2022-11-15 | Life Technologies Corporation | Chemical sensor array having multiple sensors per well |
US10816504B2 (en) | 2013-06-10 | 2020-10-27 | Life Technologies Corporation | Chemical sensor array having multiple sensors per well |
US10393700B2 (en) | 2013-10-17 | 2019-08-27 | Roche Sequencing Solutions, Inc. | Non-faradaic, capacitively coupled measurement in a nanopore cell array |
US9551697B2 (en) | 2013-10-17 | 2017-01-24 | Genia Technologies, Inc. | Non-faradaic, capacitively coupled measurement in a nanopore cell array |
US11021745B2 (en) | 2013-10-23 | 2021-06-01 | Roche Sequencing Solutions, Inc. | Methods for forming lipid bilayers on biochips |
US10421995B2 (en) | 2013-10-23 | 2019-09-24 | Genia Technologies, Inc. | High speed molecular sensing with nanopores |
US9567630B2 (en) | 2013-10-23 | 2017-02-14 | Genia Technologies, Inc. | Methods for forming lipid bilayers on biochips |
US9322062B2 (en) | 2013-10-23 | 2016-04-26 | Genia Technologies, Inc. | Process for biosensor well formation |
US9388193B2 (en) | 2014-05-22 | 2016-07-12 | National Health Research Institutes | Dipicolylamine derivatives and their pharmaceutical uses |
US10379079B2 (en) | 2014-12-18 | 2019-08-13 | Life Technologies Corporation | Methods and apparatus for measuring analytes using large scale FET arrays |
US10767224B2 (en) | 2014-12-18 | 2020-09-08 | Life Technologies Corporation | High data rate integrated circuit with power management |
US10077472B2 (en) | 2014-12-18 | 2018-09-18 | Life Technologies Corporation | High data rate integrated circuit with power management |
US11536688B2 (en) | 2014-12-18 | 2022-12-27 | Life Technologies Corporation | High data rate integrated circuit with transmitter configuration |
US10605767B2 (en) | 2014-12-18 | 2020-03-31 | Life Technologies Corporation | High data rate integrated circuit with transmitter configuration |
US9890426B2 (en) | 2015-03-09 | 2018-02-13 | The Trustees Of Columbia University In The City Of New York | Pore-forming protein conjugate compositions and methods |
WO2019027326A2 (en) | 2017-08-04 | 2019-02-07 | Universiteit Leiden | Screening method |
WO2022019837A1 (en) * | 2020-07-21 | 2022-01-27 | Agency For Science, Technology And Research | A pyrosequencing method |
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US20160312279A1 (en) | 2016-10-27 |
US9909177B2 (en) | 2018-03-06 |
US9169510B2 (en) | 2015-10-27 |
US20090325154A1 (en) | 2009-12-31 |
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